AlfalfaGEDB | MS.gene01275

M. sativa cultivar XinJiangDaYe / MS.gene01275

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01275.t1	XP_003593361.1	92.7	151	10	1	1	151	16	165	4.90E-80	307

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01275.t1	Q9ZNS4	61.8	144	47	4	8	151	26	161	8.5e-49	194.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01275.t1	G7IFD4	92.7	151	10	1	1	151	16	165	3.5e-80	307.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene01275	MS.gene02252	PPI
MS.gene01275	MS.gene002293	PPI
MS.gene01684	MS.gene01275	PPI
MS.gene02255	MS.gene01275	PPI
MS.gene00249	MS.gene01275	PPI
MS.gene01275	MS.gene57900	PPI
MS.gene02254	MS.gene01275	PPI
MS.gene01273	MS.gene01275	PPI
MS.gene018353	MS.gene01275	PPI
MS.gene002299	MS.gene01275	PPI
MS.gene01275	MS.gene01270	PPI
MS.gene01275	MS.gene002296	PPI
MS.gene01275	MS.gene002297	PPI
MS.gene01275	MS.gene01690	PPI
MS.gene01272	MS.gene01275	PPI
MS.gene01688	MS.gene01275	PPI
MS.gene01275	MS.gene002295	PPI
MS.gene01275	MS.gene002294	PPI
MS.gene01275	MS.gene01274	PPI
MS.gene02249	MS.gene01275	PPI
MS.gene01275	MS.gene01356	PPI
MS.gene01275	MS.gene002292	PPI
MS.gene012420	MS.gene01275	PPI
MS.gene01275	MS.gene01277	PPI
MS.gene01275	MS.gene02256	PPI
MS.gene01687	MS.gene01275	PPI
MS.gene01275	MS.gene01686	PPI
MS.gene01275	MS.gene01685	PPI
MS.gene01275	MS.gene035687	PPI
MS.gene035685	MS.gene01275	PPI
MS.gene01275	MS.gene02258	PPI
MS.gene028545	MS.gene01275	PPI
MS.gene01275	MS.gene038936	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01275.t1	MTR_2g010630	92.715	151	10	1	1	151	16	165	2.30e-104	295
MS.gene01275.t1	MTR_2g010650	89.933	149	12	3	3	151	20	165	8.11e-95	271
MS.gene01275.t1	MTR_2g010600	75.168	149	32	2	4	151	18	162	2.68e-82	239
MS.gene01275.t1	MTR_4g050762	73.826	149	34	2	4	151	18	162	1.40e-79	233
MS.gene01275.t1	MTR_2g012370	73.826	149	34	2	4	151	18	162	1.40e-79	233
MS.gene01275.t1	MTR_2g010700	70.323	155	40	3	1	151	16	168	1.16e-75	223
MS.gene01275.t1	MTR_2g010590	67.568	148	42	2	4	151	18	159	2.46e-74	219
MS.gene01275.t1	MTR_2g010670	68.212	151	46	2	1	151	16	164	4.70e-70	208
MS.gene01275.t1	MTR_2g010640	67.550	151	46	3	1	151	16	163	1.52e-69	207
MS.gene01275.t1	MTR_2g010690	65.563	151	50	2	1	151	16	164	6.08e-68	203
MS.gene01275.t1	MTR_2g010610	62.416	149	27	4	4	151	18	138	2.44e-59	180
MS.gene01275.t1	MTR_2g435490	55.333	150	60	3	3	151	21	164	1.70e-57	177
MS.gene01275.t1	MTR_2g010580	51.020	147	65	3	5	151	25	164	2.09e-51	161
MS.gene01275.t1	MTR_4g092540	47.368	152	72	4	1	151	15	159	2.43e-46	148
MS.gene01275.t1	MTR_4g128750	56.115	139	53	4	15	151	41	173	8.58e-46	147
MS.gene01275.t1	MTR_4g128770	48.936	141	65	4	14	151	70	206	2.03e-45	147
MS.gene01275.t1	MTR_1g067290	38.298	141	77	5	14	150	73	207	1.19e-28	105
MS.gene01275.t1	MTR_8g078770	35.915	142	82	6	14	151	36	172	2.78e-22	87.4
MS.gene01275.t1	MTR_5g018770	33.333	144	85	6	14	151	47	185	5.26e-19	79.3
MS.gene01275.t1	MTR_4g092515	50.633	79	32	3	6	83	24	96	1.35e-18	77.0
MS.gene01275.t1	MTR_5g018755	37.500	88	44	3	68	151	23	103	5.44e-13	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01275.t1	AT4G33720	61.806	144	49	2	8	151	26	163	4.56e-63	191
MS.gene01275.t1	AT5G26130	59.722	144	54	1	8	151	27	166	2.08e-60	184
MS.gene01275.t1	AT2G14580	61.806	144	47	4	8	151	26	161	5.92e-60	183
MS.gene01275.t1	AT2G14610	59.589	146	51	4	6	151	24	161	7.09e-59	180
MS.gene01275.t1	AT4G33710	59.028	144	55	1	8	151	27	166	7.45e-59	180
MS.gene01275.t1	AT3G19690	58.219	146	53	3	8	151	22	161	3.48e-57	176
MS.gene01275.t1	AT1G50060	55.102	147	59	3	6	151	21	161	7.80e-52	162
MS.gene01275.t1	AT4G33730	52.000	150	64	4	3	151	30	172	1.65e-47	152
MS.gene01275.t1	AT4G25780	51.064	141	62	3	14	151	54	190	1.91e-47	152
MS.gene01275.t1	AT4G25790	50.000	140	62	4	14	151	77	210	5.21e-46	149
MS.gene01275.t1	AT5G57625	51.079	139	60	4	15	151	75	207	3.31e-45	147
MS.gene01275.t1	AT4G30320	49.645	141	66	4	13	151	24	161	1.01e-43	142
MS.gene01275.t1	AT1G50050	51.370	146	65	3	7	151	22	162	1.63e-43	141
MS.gene01275.t1	AT2G19990	48.227	141	66	3	12	151	42	176	5.40e-43	140
MS.gene01275.t1	AT3G09590	48.227	141	64	4	14	151	52	186	1.14e-42	140
MS.gene01275.t1	AT5G02730	47.887	142	65	4	13	151	58	193	6.09e-40	133
MS.gene01275.t1	AT1G01310	46.043	139	68	3	14	151	87	219	4.09e-39	132
MS.gene01275.t1	AT1G50050	50.725	138	62	3	7	143	22	154	4.96e-39	132
MS.gene01275.t1	AT4G07820	43.750	144	73	4	8	151	25	160	5.06e-37	124
MS.gene01275.t1	AT4G31470	42.446	139	73	3	14	151	53	185	3.54e-36	123
MS.gene01275.t1	AT5G66590	40.278	144	72	5	15	151	49	185	2.20e-29	106
MS.gene01275.t1	AT2G19980	41.096	146	65	6	12	151	35	165	7.10e-26	96.7
MS.gene01275.t1	AT2G19970	39.073	151	73	8	12	151	35	177	2.93e-23	90.1

Find 39 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTTGTCCCAAACAATATT+TGG	0.192023	2.2:+70879776	None:intergenic
TTAAAATTCCAAATATTGTT+TGG	0.293275	2.2:-70879784	MS.gene01275:CDS
TAAAATTCCAAATATTGTTT+GGG	0.350860	2.2:-70879783	MS.gene01275:CDS
GCCACTATACTCAGGTTGTT+TGG	0.351947	2.2:-70879550	MS.gene01275:CDS
TTACTACTATAATCATAGTT+TGG	0.369699	2.2:+70879599	None:intergenic
TTGATCCACTCCGGTGGTGG+TGG	0.379754	2.2:-70879701	MS.gene01275:CDS
CGTAGTCTGCTGGAGAGTCT+TGG	0.417390	2.2:+70879849	None:intergenic
TCCGGTGGTGGTGGGCGTTA+CGG	0.427305	2.2:-70879692	MS.gene01275:CDS
GCCAAAGTGAAATGTGATAA+TGG	0.427399	2.2:-70879503	MS.gene01275:CDS
GTAGTCTGCTGGAGAGTCTT+GGG	0.435345	2.2:+70879850	None:intergenic
AGTAGTAACTCATGTACTAA+TGG	0.438033	2.2:-70879584	MS.gene01275:CDS
TGGAGAAACACAAAGCGTAT+TGG	0.455942	2.2:-70879530	MS.gene01275:CDS
TCCATTATCACATTTCACTT+TGG	0.469667	2.2:+70879502	None:intergenic
CTTTCGCCAATATAGTTGCC+AGG	0.474936	2.2:+70879449	None:intergenic
TGGGCATTCACGTAGTCTGC+TGG	0.475357	2.2:+70879839	None:intergenic
TCCGTAACGCCCACCACCAC+CGG	0.485057	2.2:+70879691	None:intergenic
TGCCGATCTTGCATTGTTGT+GGG	0.491310	2.2:+70879820	None:intergenic
CTGCCGATCTTGCATTGTTG+TGG	0.503037	2.2:+70879819	None:intergenic
TCCAAACAACCTGAGTATAG+TGG	0.509539	2.2:+70879549	None:intergenic
TGCCCACAACAATGCAAGAT+CGG	0.519302	2.2:-70879822	MS.gene01275:CDS
TGATCCACTCCGGTGGTGGT+GGG	0.526774	2.2:-70879700	MS.gene01275:CDS
CAATTGATCCACTCCGGTGG+TGG	0.548354	2.2:-70879704	MS.gene01275:CDS
TTTCGCCAATATAGTTGCCA+GGG	0.556325	2.2:+70879450	None:intergenic
GCATCGAAGCAGTGAAGTTG+TGG	0.565988	2.2:-70879634	MS.gene01275:CDS
AAGCTATGCTAATCAACGCA+AGG	0.587995	2.2:-70879735	MS.gene01275:CDS
CATCGAAGCAGTGAAGTTGT+GGG	0.588926	2.2:-70879633	MS.gene01275:CDS
CGCCAATATAGTTGCCAGGG+GGG	0.599696	2.2:+70879453	None:intergenic
AACGCCCACCACCACCGGAG+TGG	0.599839	2.2:+70879696	None:intergenic
GAGAGCAGCGGCGACATGAG+TGG	0.600653	2.2:-70879656	MS.gene01275:CDS
GAAAATATCGCAGAGAGCAG+CGG	0.617856	2.2:-70879668	MS.gene01275:CDS
TTCGCCAATATAGTTGCCAG+GGG	0.619900	2.2:+70879451	None:intergenic
AGCTATGCTAATCAACGCAA+GGG	0.628360	2.2:-70879734	MS.gene01275:CDS
GATTGTCAATTGATCCACTC+CGG	0.634101	2.2:-70879710	MS.gene01275:CDS
CGAAGCAGTGAAGTTGTGGG+TGG	0.656466	2.2:-70879630	MS.gene01275:CDS
TCGCCAATATAGTTGCCAGG+GGG	0.658113	2.2:+70879452	None:intergenic
TGTCAATTGATCCACTCCGG+TGG	0.707723	2.2:-70879707	MS.gene01275:CDS
TGTACTAATGGTGAAATGTG+TGG	0.716008	2.2:-70879572	MS.gene01275:CDS
AATGTGTGGCCACTATACTC+AGG	0.735120	2.2:-70879558	MS.gene01275:CDS
AAAGTGAAATGTGATAATGG+AGG	0.772799	2.2:-70879500	MS.gene01275:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATTCCAAATATTGTTT+GGG	-	chr2.2:70879528-70879547	MS.gene01275:CDS	15.0%
!!	TTAAAATTCCAAATATTGTT+TGG	-	chr2.2:70879527-70879546	MS.gene01275:CDS	15.0%
!!	TTACTACTATAATCATAGTT+TGG	+	chr2.2:70879715-70879734	None:intergenic	20.0%
	AGTAGTAACTCATGTACTAA+TGG	-	chr2.2:70879727-70879746	MS.gene01275:CDS	30.0%
	TCCATTATCACATTTCACTT+TGG	+	chr2.2:70879812-70879831	None:intergenic	30.0%
	TTGTTGTCCCAAACAATATT+TGG	+	chr2.2:70879538-70879557	None:intergenic	30.0%
!!	AAAGTGAAATGTGATAATGG+AGG	-	chr2.2:70879811-70879830	MS.gene01275:CDS	30.0%
	GCCAAAGTGAAATGTGATAA+TGG	-	chr2.2:70879808-70879827	MS.gene01275:CDS	35.0%
	TGTACTAATGGTGAAATGTG+TGG	-	chr2.2:70879739-70879758	MS.gene01275:CDS	35.0%
	AAGCTATGCTAATCAACGCA+AGG	-	chr2.2:70879576-70879595	MS.gene01275:CDS	40.0%
	AGCTATGCTAATCAACGCAA+GGG	-	chr2.2:70879577-70879596	MS.gene01275:CDS	40.0%
	GATTGTCAATTGATCCACTC+CGG	-	chr2.2:70879601-70879620	MS.gene01275:CDS	40.0%
	TCCAAACAACCTGAGTATAG+TGG	+	chr2.2:70879765-70879784	None:intergenic	40.0%
	TGGAGAAACACAAAGCGTAT+TGG	-	chr2.2:70879781-70879800	MS.gene01275:CDS	40.0%
	TTTCGCCAATATAGTTGCCA+GGG	+	chr2.2:70879864-70879883	None:intergenic	40.0%
	AATGTGTGGCCACTATACTC+AGG	-	chr2.2:70879753-70879772	MS.gene01275:CDS	45.0%
	CATCGAAGCAGTGAAGTTGT+GGG	-	chr2.2:70879678-70879697	MS.gene01275:CDS	45.0%
	CTTTCGCCAATATAGTTGCC+AGG	+	chr2.2:70879865-70879884	None:intergenic	45.0%
	GAAAATATCGCAGAGAGCAG+CGG	-	chr2.2:70879643-70879662	MS.gene01275:CDS	45.0%
	TGCCCACAACAATGCAAGAT+CGG	-	chr2.2:70879489-70879508	MS.gene01275:CDS	45.0%
	TGCCGATCTTGCATTGTTGT+GGG	+	chr2.2:70879494-70879513	None:intergenic	45.0%
	TTCGCCAATATAGTTGCCAG+GGG	+	chr2.2:70879863-70879882	None:intergenic	45.0%
!	GCCACTATACTCAGGTTGTT+TGG	-	chr2.2:70879761-70879780	MS.gene01275:CDS	45.0%
	CTGCCGATCTTGCATTGTTG+TGG	+	chr2.2:70879495-70879514	None:intergenic	50.0%
	GCATCGAAGCAGTGAAGTTG+TGG	-	chr2.2:70879677-70879696	MS.gene01275:CDS	50.0%
	GTAGTCTGCTGGAGAGTCTT+GGG	+	chr2.2:70879464-70879483	None:intergenic	50.0%
	TCGCCAATATAGTTGCCAGG+GGG	+	chr2.2:70879862-70879881	None:intergenic	50.0%
	TGTCAATTGATCCACTCCGG+TGG	-	chr2.2:70879604-70879623	MS.gene01275:CDS	50.0%
	ACTTGCAATTACGACCCCCC+TGG	-	chr2.2:70879844-70879863	MS.gene01275:CDS	55.0%
	CAATTGATCCACTCCGGTGG+TGG	-	chr2.2:70879607-70879626	MS.gene01275:CDS	55.0%
	CGAAGCAGTGAAGTTGTGGG+TGG	-	chr2.2:70879681-70879700	MS.gene01275:CDS	55.0%
	CGCCAATATAGTTGCCAGGG+GGG	+	chr2.2:70879861-70879880	None:intergenic	55.0%
	CGTAGTCTGCTGGAGAGTCT+TGG	+	chr2.2:70879465-70879484	None:intergenic	55.0%
	GACCCCCCTGGCAACTATAT+TGG	-	chr2.2:70879856-70879875	MS.gene01275:CDS	55.0%
	TGGGCATTCACGTAGTCTGC+TGG	+	chr2.2:70879475-70879494	None:intergenic	55.0%
!	TGATCCACTCCGGTGGTGGT+GGG	-	chr2.2:70879611-70879630	MS.gene01275:CDS	60.0%
!	TTGATCCACTCCGGTGGTGG+TGG	-	chr2.2:70879610-70879629	MS.gene01275:CDS	60.0%
	GAGAGCAGCGGCGACATGAG+TGG	-	chr2.2:70879655-70879674	MS.gene01275:CDS	65.0%
	TCCGTAACGCCCACCACCAC+CGG	+	chr2.2:70879623-70879642	None:intergenic	65.0%
!!	TCCGGTGGTGGTGGGCGTTA+CGG	-	chr2.2:70879619-70879638	MS.gene01275:CDS	65.0%
	AACGCCCACCACCACCGGAG+TGG	+	chr2.2:70879618-70879637	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	70879439	70879894	70879439	ID=MS.gene01275
chr2.2	mRNA	70879439	70879894	70879439	ID=MS.gene01275.t1;Parent=MS.gene01275
chr2.2	exon	70879439	70879894	70879439	ID=MS.gene01275.t1.exon1;Parent=MS.gene01275.t1
chr2.2	CDS	70879439	70879894	70879439	ID=cds.MS.gene01275.t1;Parent=MS.gene01275.t1

Gene Sequence

>MS.gene01275

ATGGGTAGTCACGTTGCACATGCCCAAGACTCTCCAGCAGACTACGTGAATGCCCACAACAATGCAAGATCGGCAGTTCATACAAACGTTAAAATTCCAAATATTGTTTGGGACAACAAAGTTGCTGCTTTTGCACAAAGCTATGCTAATCAACGCAAGGGTGATTGTCAATTGATCCACTCCGGTGGTGGTGGGCGTTACGGAGAAAATATCGCAGAGAGCAGCGGCGACATGAGTGGCATCGAAGCAGTGAAGTTGTGGGTGGATGAAAAACCAAACTATGATTATAGTAGTAACTCATGTACTAATGGTGAAATGTGTGGCCACTATACTCAGGTTGTTTGGAGAAACACAAAGCGTATTGGATGTGCCAAAGTGAAATGTGATAATGGAGGCACATTCATTACTTGCAATTACGACCCCCCTGGCAACTATATTGGCGAAAGACCTTACTAA

Protein sequence

>MS.gene01275.t1

MGSHVAHAQDSPADYVNAHNNARSAVHTNVKIPNIVWDNKVAAFAQSYANQRKGDCQLIHSGGGGRYGENIAESSGDMSGIEAVKLWVDEKPNYDYSSNSCTNGEMCGHYTQVVWRNTKRIGCAKVKCDNGGTFITCNYDPPGNYIGERPY