AlfalfaGEDB | MS.gene01686

M. sativa cultivar XinJiangDaYe / MS.gene01686

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01686.t1	RHN71757.1	90.2	163	15	1	1	163	274	435	4.80E-81	310.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01686.t1	Q9ZNS4	64.2	165	51	4	1	163	3	161	9.5e-54	211.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01686.t1	G7IFD1	90.2	163	15	1	1	163	1	162	3.4e-81	310.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene00249	MS.gene01686	PPI
MS.gene01686	MS.gene01274	PPI
MS.gene02256	MS.gene01686	PPI
MS.gene01686	MS.gene002296	PPI
MS.gene02254	MS.gene01686	PPI
MS.gene01273	MS.gene01686	PPI
MS.gene018353	MS.gene01686	PPI
MS.gene01686	MS.gene01277	PPI
MS.gene002299	MS.gene01686	PPI
MS.gene01686	MS.gene02256	PPI
MS.gene01686	MS.gene002295	PPI
MS.gene002295	MS.gene01686	PPI
MS.gene01686	MS.gene002294	PPI
MS.gene01272	MS.gene01686	PPI
MS.gene01688	MS.gene01686	PPI
MS.gene01356	MS.gene01686	PPI
MS.gene01686	MS.gene02258	PPI
MS.gene01686	MS.gene002292	PPI
MS.gene02249	MS.gene01686	PPI
MS.gene012420	MS.gene01686	PPI
MS.gene01686	MS.gene01685	PPI
MS.gene01687	MS.gene01686	PPI
MS.gene02252	MS.gene01686	PPI
MS.gene035687	MS.gene01686	PPI
MS.gene01690	MS.gene01686	PPI
MS.gene035685	MS.gene01686	PPI
MS.gene01686	MS.gene038936	PPI
MS.gene01275	MS.gene01686	PPI
MS.gene01686	MS.gene57900	PPI
MS.gene01686	MS.gene02252	PPI
MS.gene028545	MS.gene01686	PPI
MS.gene01686	MS.gene002293	PPI
MS.gene01686	MS.gene01270	PPI
MS.gene01686	MS.gene002297	PPI
MS.gene01684	MS.gene01686	PPI
MS.gene02255	MS.gene01686	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01686.t1	MTR_2g010600	90.184	163	15	1	1	163	1	162	1.88e-108	306
MS.gene01686.t1	MTR_4g050762	84.049	163	25	1	1	163	1	162	1.50e-94	271
MS.gene01686.t1	MTR_2g012370	84.049	163	25	1	1	163	1	162	1.50e-94	271
MS.gene01686.t1	MTR_2g010590	74.847	163	37	2	1	163	1	159	7.01e-89	256
MS.gene01686.t1	MTR_2g010650	67.647	170	43	7	1	163	1	165	2.28e-73	217
MS.gene01686.t1	MTR_2g010610	72.393	163	20	3	1	163	1	138	3.40e-72	213
MS.gene01686.t1	MTR_2g010630	69.872	156	39	5	13	163	13	165	9.33e-72	213
MS.gene01686.t1	MTR_2g010700	59.412	170	60	5	1	163	1	168	1.79e-65	197
MS.gene01686.t1	MTR_2g435490	54.717	159	68	2	6	163	9	164	4.96e-63	192
MS.gene01686.t1	MTR_2g010640	60.479	167	58	6	1	163	1	163	7.38e-61	186
MS.gene01686.t1	MTR_2g010670	57.738	168	62	6	1	163	1	164	1.16e-55	172
MS.gene01686.t1	MTR_2g010690	54.762	168	67	6	1	163	1	164	4.43e-53	166
MS.gene01686.t1	MTR_2g010580	50.331	151	70	3	13	163	19	164	5.72e-52	163
MS.gene01686.t1	MTR_4g092540	45.122	164	84	4	1	163	1	159	8.92e-45	145
MS.gene01686.t1	MTR_4g128750	50.735	136	63	2	29	163	41	173	2.17e-44	144
MS.gene01686.t1	MTR_4g128770	44.203	138	74	3	28	163	70	206	1.37e-38	130
MS.gene01686.t1	MTR_1g067290	42.029	138	74	4	28	162	73	207	6.19e-33	116
MS.gene01686.t1	MTR_8g078770	38.129	139	81	4	28	163	36	172	8.12e-26	97.1
MS.gene01686.t1	MTR_5g018770	31.844	179	103	4	4	163	7	185	1.14e-24	94.4
MS.gene01686.t1	MTR_4g092515	55.789	95	36	4	1	94	7	96	7.27e-23	88.2
MS.gene01686.t1	MTR_5g018755	38.462	91	51	4	78	163	13	103	1.13e-13	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01686.t1	AT2G14580	64.242	165	51	4	1	163	3	161	5.09e-70	209
MS.gene01686.t1	AT4G33720	59.259	162	62	2	2	163	6	163	5.18e-66	199
MS.gene01686.t1	AT2G14610	58.788	165	60	4	1	163	3	161	4.65e-64	194
MS.gene01686.t1	AT5G26130	58.228	158	64	2	6	163	11	166	1.35e-59	182
MS.gene01686.t1	AT3G19690	53.333	165	71	3	1	163	1	161	4.94e-59	181
MS.gene01686.t1	AT4G33710	59.859	142	55	2	22	163	27	166	1.95e-55	172
MS.gene01686.t1	AT2G19990	54.962	131	56	1	33	163	49	176	4.18e-50	159
MS.gene01686.t1	AT1G50060	52.410	166	71	5	1	163	1	161	7.84e-50	157
MS.gene01686.t1	AT4G25790	49.640	139	66	2	26	163	75	210	9.41e-48	154
MS.gene01686.t1	AT3G09590	48.551	138	66	2	28	163	52	186	4.63e-46	149
MS.gene01686.t1	AT5G57625	49.265	136	65	2	29	163	75	207	7.36e-46	149
MS.gene01686.t1	AT1G50050	53.012	166	71	5	1	163	1	162	9.81e-46	147
MS.gene01686.t1	AT4G30320	45.033	151	78	3	14	163	15	161	6.19e-44	142
MS.gene01686.t1	AT4G25780	50.000	138	66	3	28	163	54	190	3.49e-43	142
MS.gene01686.t1	AT4G33730	48.428	159	68	5	14	163	19	172	3.82e-43	141
MS.gene01686.t1	AT1G50050	51.829	164	72	5	1	161	1	160	1.66e-42	141
MS.gene01686.t1	AT5G02730	47.482	139	68	2	27	163	58	193	2.13e-42	140
MS.gene01686.t1	AT1G01310	46.715	137	68	3	28	163	87	219	1.14e-39	134
MS.gene01686.t1	AT4G31470	44.604	139	72	3	26	163	51	185	2.28e-38	129
MS.gene01686.t1	AT4G07820	46.479	142	70	4	22	163	25	160	1.08e-37	127
MS.gene01686.t1	AT5G66590	42.336	137	77	1	29	163	49	185	5.00e-34	118
MS.gene01686.t1	AT2G19970	43.262	141	71	5	30	163	39	177	1.27e-29	107
MS.gene01686.t1	AT2G19980	37.427	171	85	8	3	163	7	165	6.46e-26	97.1

Find 38 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTGGCGGTGATAAACATTA+TGG	0.283064	2.4:-72712676	MS.gene01686:CDS
GCTGGGCTTATCCCTCATTA+TGG	0.334186	2.4:-72712863	MS.gene01686:CDS
CATAGTCTGCTTGTGAGTCT+TGG	0.335837	2.4:+72712824	None:intergenic
TATTAAGCATGCACTTAGTA+TGG	0.353177	2.4:+72712406	None:intergenic
GCCATTATACTCAGGTGGTT+TGG	0.358578	2.4:-72712534	MS.gene01686:CDS
TCCAAACCACCTGAGTATAA+TGG	0.398812	2.4:+72712533	None:intergenic
ATAGTCTGCTTGTGAGTCTT+GGG	0.401577	2.4:+72712825	None:intergenic
TTGGTGTTGCAAATATTGTT+TGG	0.407510	2.4:-72712771	MS.gene01686:CDS
GTTACTATTATAATCATAGT+TGG	0.429625	2.4:+72712582	None:intergenic
TGGTGTTGCAAATATTGTTT+GGG	0.451345	2.4:-72712770	MS.gene01686:CDS
TTGACGTCTTGCATCGTTGT+GGG	0.459049	2.4:+72712795	None:intergenic
GTGGCGGTGATAAACATTAT+GGG	0.460708	2.4:-72712675	MS.gene01686:CDS
ATTATTATCACATCTCACTT+TGG	0.463790	2.4:+72712486	None:intergenic
CGTTATTATAGTTGCCTGGT+GGG	0.465282	2.4:+72712434	None:intergenic
GAGAATCTAGCGTGGAGCTC+CGG	0.490150	2.4:-72712652	MS.gene01686:CDS
GTGAGATGTGATAATAATAG+AGG	0.514467	2.4:-72712481	MS.gene01686:CDS
ATCTGAGCCACTCATGTCGC+CGG	0.523273	2.4:+72712633	None:intergenic
CTTGACGTCTTGCATCGTTG+TGG	0.543312	2.4:+72712794	None:intergenic
GCTCAGATGCAGTGAAGTTG+TGG	0.543630	2.4:-72712618	MS.gene01686:CDS
CTCAGATGCAGTGAAGTTGT+GGG	0.551141	2.4:-72712617	MS.gene01686:CDS
TGGAGCTCCGGCGACATGAG+TGG	0.557633	2.4:-72712640	MS.gene01686:CDS
GTGTAACATGAACCATAATG+AGG	0.566235	2.4:+72712851	None:intergenic
ATAATGTTTATCACCGCCAC+CGG	0.571825	2.4:+72712678	None:intergenic
TCGTTATTATAGTTGCCTGG+TGG	0.573306	2.4:+72712433	None:intergenic
AAACTATGCTAATCAACGCA+AGG	0.596763	2.4:-72712722	MS.gene01686:CDS
TGGAGAAACTCAGTACGTGT+TGG	0.598633	2.4:-72712514	MS.gene01686:CDS
ACATGCAATTACGACCCACC+AGG	0.608369	2.4:-72712448	MS.gene01686:CDS
AACTATGCTAATCAACGCAA+GGG	0.616329	2.4:-72712721	MS.gene01686:CDS
TGTAACATGAACCATAATGA+GGG	0.618907	2.4:+72712852	None:intergenic
AACGATGCAAGACGTCAAGT+TGG	0.620216	2.4:-72712790	MS.gene01686:CDS
AAACTGATGCACTCCGGTGG+CGG	0.623369	2.4:-72712691	MS.gene01686:CDS
GTGTGGCCATTATACTCAGG+TGG	0.627875	2.4:-72712539	MS.gene01686:CDS
TGGCGGTGATAAACATTATG+GGG	0.629775	2.4:-72712674	MS.gene01686:CDS
AGTGTGTGGCCATTATACTC+AGG	0.651003	2.4:-72712542	MS.gene01686:CDS
GATTGTAAACTGATGCACTC+CGG	0.660976	2.4:-72712697	MS.gene01686:CDS
TGTAAACTGATGCACTCCGG+TGG	0.705347	2.4:-72712694	MS.gene01686:CDS
ATTATGGGGAGAATCTAGCG+TGG	0.720756	2.4:-72712660	MS.gene01686:CDS
TGTGCTGCTAATAAAGTGTG+TGG	0.730769	2.4:-72712556	MS.gene01686:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTACTATTATAATCATAGT+TGG	+	chr2.4:72712730-72712749	None:intergenic	20.0%
!	ATTATTATCACATCTCACTT+TGG	+	chr2.4:72712826-72712845	None:intergenic	25.0%
	GTGAGATGTGATAATAATAG+AGG	-	chr2.4:72712828-72712847	MS.gene01686:CDS	30.0%
	TGTAACATGAACCATAATGA+GGG	+	chr2.4:72712460-72712479	None:intergenic	30.0%
!	TTTTCGTTATTATAGTTGCC+TGG	+	chr2.4:72712882-72712901	None:intergenic	30.0%
!!	TGGTGTTGCAAATATTGTTT+GGG	-	chr2.4:72712539-72712558	MS.gene01686:CDS	30.0%
!!	TTGGTGTTGCAAATATTGTT+TGG	-	chr2.4:72712538-72712557	MS.gene01686:CDS	30.0%
	AAACTATGCTAATCAACGCA+AGG	-	chr2.4:72712587-72712606	MS.gene01686:CDS	35.0%
	AACTATGCTAATCAACGCAA+GGG	-	chr2.4:72712588-72712607	MS.gene01686:CDS	35.0%
	GTGTAACATGAACCATAATG+AGG	+	chr2.4:72712461-72712480	None:intergenic	35.0%
!	ATTTTCTCTATTGTGTGTGC+TGG	-	chr2.4:72712428-72712447	MS.gene01686:CDS	35.0%
!	TTTTCTCTATTGTGTGTGCT+GGG	-	chr2.4:72712429-72712448	MS.gene01686:CDS	35.0%
	ATAATGTTTATCACCGCCAC+CGG	+	chr2.4:72712634-72712653	None:intergenic	40.0%
	ATAGTCTGCTTGTGAGTCTT+GGG	+	chr2.4:72712487-72712506	None:intergenic	40.0%
	CGTTATTATAGTTGCCTGGT+GGG	+	chr2.4:72712878-72712897	None:intergenic	40.0%
	GATTGTAAACTGATGCACTC+CGG	-	chr2.4:72712612-72712631	MS.gene01686:CDS	40.0%
	GTGGCGGTGATAAACATTAT+GGG	-	chr2.4:72712634-72712653	MS.gene01686:CDS	40.0%
	TCCAAACCACCTGAGTATAA+TGG	+	chr2.4:72712779-72712798	None:intergenic	40.0%
	TCGTTATTATAGTTGCCTGG+TGG	+	chr2.4:72712879-72712898	None:intergenic	40.0%
	TGGCGGTGATAAACATTATG+GGG	-	chr2.4:72712635-72712654	MS.gene01686:CDS	40.0%
!	TGTGCTGCTAATAAAGTGTG+TGG	-	chr2.4:72712753-72712772	MS.gene01686:CDS	40.0%
	AACGATGCAAGACGTCAAGT+TGG	-	chr2.4:72712519-72712538	MS.gene01686:CDS	45.0%
	AGTGTGTGGCCATTATACTC+AGG	-	chr2.4:72712767-72712786	MS.gene01686:CDS	45.0%
	ATTATGGGGAGAATCTAGCG+TGG	-	chr2.4:72712649-72712668	MS.gene01686:CDS	45.0%
	CATAGTCTGCTTGTGAGTCT+TGG	+	chr2.4:72712488-72712507	None:intergenic	45.0%
	CTCAGATGCAGTGAAGTTGT+GGG	-	chr2.4:72712692-72712711	MS.gene01686:CDS	45.0%
	GCCATTATACTCAGGTGGTT+TGG	-	chr2.4:72712775-72712794	MS.gene01686:CDS	45.0%
	TGGAGAAACTCAGTACGTGT+TGG	-	chr2.4:72712795-72712814	MS.gene01686:CDS	45.0%
	TTGACGTCTTGCATCGTTGT+GGG	+	chr2.4:72712517-72712536	None:intergenic	45.0%
!	GGTGGCGGTGATAAACATTA+TGG	-	chr2.4:72712633-72712652	MS.gene01686:CDS	45.0%
	ACATGCAATTACGACCCACC+AGG	-	chr2.4:72712861-72712880	MS.gene01686:CDS	50.0%
	CTTGACGTCTTGCATCGTTG+TGG	+	chr2.4:72712518-72712537	None:intergenic	50.0%
	GCTCAGATGCAGTGAAGTTG+TGG	-	chr2.4:72712691-72712710	MS.gene01686:CDS	50.0%
	GCTGGGCTTATCCCTCATTA+TGG	-	chr2.4:72712446-72712465	MS.gene01686:CDS	50.0%
	GTGTGGCCATTATACTCAGG+TGG	-	chr2.4:72712770-72712789	MS.gene01686:CDS	50.0%
	TGTAAACTGATGCACTCCGG+TGG	-	chr2.4:72712615-72712634	MS.gene01686:CDS	50.0%
	AAACTGATGCACTCCGGTGG+CGG	-	chr2.4:72712618-72712637	MS.gene01686:CDS	55.0%
	ATCTGAGCCACTCATGTCGC+CGG	+	chr2.4:72712679-72712698	None:intergenic	55.0%
	GAGAATCTAGCGTGGAGCTC+CGG	-	chr2.4:72712657-72712676	MS.gene01686:CDS	55.0%
	TGGAGCTCCGGCGACATGAG+TGG	-	chr2.4:72712669-72712688	MS.gene01686:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	72712420	72712911	72712420	ID=MS.gene01686
chr2.4	mRNA	72712420	72712911	72712420	ID=MS.gene01686.t1;Parent=MS.gene01686
chr2.4	exon	72712420	72712911	72712420	ID=MS.gene01686.t1.exon1;Parent=MS.gene01686.t1
chr2.4	CDS	72712420	72712911	72712420	ID=cds.MS.gene01686.t1;Parent=MS.gene01686.t1

Gene Sequence

>MS.gene01686

ATGAGTTCATTTTCTCTATTGTGTGTGCTGGGCTTATCCCTCATTATGGTTCATGTTACACATGCCCAAGACTCACAAGCAGACTATGTGAACGCCCACAACGATGCAAGACGTCAAGTTGGTGTTGCAAATATTGTTTGGGACAATACTGTTGCTGCTTTTGCACAAAACTATGCTAATCAACGCAAGGGTGATTGTAAACTGATGCACTCCGGTGGCGGTGATAAACATTATGGGGAGAATCTAGCGTGGAGCTCCGGCGACATGAGTGGCTCAGATGCAGTGAAGTTGTGGGTGAACGAGAAAGCCAACTATGATTATAATAGTAACACATGTGCTGCTAATAAAGTGTGTGGCCATTATACTCAGGTGGTTTGGAGAAACTCAGTACGTGTTGGATGTGCCAAAGTGAGATGTGATAATAATAGAGGAACATTCATTACATGCAATTACGACCCACCAGGCAACTATAATAACGAAAAGCCATACTAA

Protein sequence

>MS.gene01686.t1

MSSFSLLCVLGLSLIMVHVTHAQDSQADYVNAHNDARRQVGVANIVWDNTVAAFAQNYANQRKGDCKLMHSGGGDKHYGENLAWSSGDMSGSDAVKLWVNEKANYDYNSNTCAANKVCGHYTQVVWRNSVRVGCAKVRCDNNRGTFITCNYDPPGNYNNEKPY