AlfalfaGEDB | MS.gene002295

M. sativa cultivar XinJiangDaYe / MS.gene002295

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002295.t1	RHN71757.1	90.7	162	15	0	1	162	274	435	1.40E-85	325.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002295.t1	Q9ZNS4	61.6	164	56	4	1	162	3	161	6.1e-53	208.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002295.t1	G7IFD1	90.7	162	15	0	1	162	1	162	1.0e-85	325.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene02255	MS.gene002295	PPI
MS.gene002295	MS.gene57900	PPI
MS.gene00249	MS.gene002295	PPI
MS.gene02256	MS.gene002295	PPI
MS.gene002295	MS.gene002296	PPI
MS.gene02254	MS.gene002295	PPI
MS.gene01273	MS.gene002295	PPI
MS.gene002295	MS.gene002297	PPI
MS.gene018353	MS.gene002295	PPI
MS.gene002295	MS.gene01277	PPI
MS.gene002299	MS.gene002295	PPI
MS.gene002295	MS.gene02256	PPI
MS.gene01686	MS.gene002295	PPI
MS.gene002295	MS.gene01686	PPI
MS.gene002295	MS.gene002294	PPI
MS.gene01272	MS.gene002295	PPI
MS.gene002295	MS.gene01274	PPI
MS.gene02252	MS.gene002295	PPI
MS.gene01688	MS.gene002295	PPI
MS.gene01356	MS.gene002295	PPI
MS.gene002295	MS.gene02258	PPI
MS.gene002295	MS.gene002292	PPI
MS.gene02249	MS.gene002295	PPI
MS.gene01275	MS.gene002295	PPI
MS.gene012420	MS.gene002295	PPI
MS.gene002295	MS.gene01685	PPI
MS.gene01687	MS.gene002295	PPI
MS.gene035687	MS.gene002295	PPI
MS.gene01690	MS.gene002295	PPI
MS.gene035685	MS.gene002295	PPI
MS.gene002295	MS.gene038936	PPI
MS.gene002295	MS.gene02252	PPI
MS.gene028545	MS.gene002295	PPI
MS.gene002295	MS.gene002293	PPI
MS.gene002295	MS.gene01270	PPI
MS.gene01684	MS.gene002295	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002295.t1	MTR_2g010600	90.741	162	15	0	1	162	1	162	4.96e-113	317
MS.gene002295.t1	MTR_4g050762	85.802	162	23	0	1	162	1	162	1.68e-100	286
MS.gene002295.t1	MTR_2g012370	85.802	162	23	0	1	162	1	162	1.68e-100	286
MS.gene002295.t1	MTR_2g010590	82.716	162	25	2	1	162	1	159	2.15e-97	278
MS.gene002295.t1	MTR_2g010630	71.613	155	37	4	13	162	13	165	8.81e-78	228
MS.gene002295.t1	MTR_2g010650	68.639	169	42	7	1	162	1	165	2.72e-76	225
MS.gene002295.t1	MTR_2g010610	71.605	162	22	3	1	162	1	138	2.02e-73	216
MS.gene002295.t1	MTR_2g010700	61.765	170	55	5	1	162	1	168	1.96e-69	207
MS.gene002295.t1	MTR_2g435490	55.696	158	67	2	6	162	9	164	1.29e-65	198
MS.gene002295.t1	MTR_2g010640	60.241	166	59	5	1	162	1	163	6.62e-64	193
MS.gene002295.t1	MTR_2g010670	59.281	167	60	5	1	162	1	164	1.85e-59	182
MS.gene002295.t1	MTR_2g010690	55.689	167	66	5	1	162	1	164	2.20e-56	174
MS.gene002295.t1	MTR_2g010580	46.000	150	77	3	13	162	19	164	4.02e-48	153
MS.gene002295.t1	MTR_4g128750	51.852	135	62	2	29	162	41	173	3.12e-46	149
MS.gene002295.t1	MTR_4g092540	44.785	163	85	4	1	162	1	159	1.10e-45	147
MS.gene002295.t1	MTR_4g128770	47.101	138	69	3	28	162	70	206	1.01e-42	141
MS.gene002295.t1	MTR_1g067290	38.686	137	79	4	28	161	73	207	8.26e-33	116
MS.gene002295.t1	MTR_8g078770	36.957	138	83	4	28	162	36	172	1.78e-25	96.3
MS.gene002295.t1	MTR_5g018770	30.000	190	91	6	4	162	7	185	7.51e-24	92.4
MS.gene002295.t1	MTR_4g092515	51.923	104	45	4	1	103	7	106	1.86e-22	87.4
MS.gene002295.t1	MTR_5g018755	30.851	94	60	3	74	162	10	103	6.25e-13	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002295.t1	AT2G14580	61.585	164	56	4	1	162	3	161	3.64e-67	201
MS.gene002295.t1	AT4G33720	59.006	161	63	2	2	162	6	163	5.04e-66	199
MS.gene002295.t1	AT5G26130	58.599	157	64	1	6	162	11	166	1.61e-63	192
MS.gene002295.t1	AT2G14610	56.707	164	64	4	1	162	3	161	2.07e-61	187
MS.gene002295.t1	AT4G33710	63.121	141	51	1	22	162	27	166	3.20e-61	187
MS.gene002295.t1	AT3G19690	53.049	164	72	3	1	162	1	161	1.45e-60	185
MS.gene002295.t1	AT4G25790	52.899	138	62	2	26	162	75	210	1.34e-50	161
MS.gene002295.t1	AT1G50060	50.909	165	74	5	1	162	1	161	6.92e-50	158
MS.gene002295.t1	AT5G57625	52.593	135	61	2	29	162	75	207	1.38e-48	156
MS.gene002295.t1	AT4G30320	48.000	150	74	3	14	162	15	161	2.25e-47	151
MS.gene002295.t1	AT3G09590	50.365	137	64	2	28	162	52	186	3.94e-47	152
MS.gene002295.t1	AT2G19990	51.538	130	61	1	33	162	49	176	4.00e-47	151
MS.gene002295.t1	AT1G50050	53.333	165	71	5	1	162	1	162	1.42e-46	149
MS.gene002295.t1	AT4G25780	50.725	138	64	3	28	162	54	190	8.48e-46	148
MS.gene002295.t1	AT4G33730	49.367	158	67	5	14	162	19	172	2.77e-45	146
MS.gene002295.t1	AT5G02730	47.826	138	68	2	27	162	58	193	1.47e-42	140
MS.gene002295.t1	AT1G50050	50.920	163	74	5	1	160	1	160	3.08e-41	138
MS.gene002295.t1	AT1G01310	45.588	136	70	3	28	162	87	219	9.16e-39	132
MS.gene002295.t1	AT4G31470	44.928	138	72	3	26	162	51	185	5.79e-38	128
MS.gene002295.t1	AT5G66590	43.571	140	70	3	29	162	49	185	4.44e-36	123
MS.gene002295.t1	AT4G07820	43.662	142	75	4	21	162	24	160	9.29e-36	122
MS.gene002295.t1	AT2G19980	35.294	170	89	8	3	162	7	165	9.42e-24	91.7
MS.gene002295.t1	AT2G19970	39.394	132	67	6	30	152	39	166	1.67e-23	91.3

Find 45 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGGTGGTGGTGGCCGTTAC+GGG	0.193193	2.3:-72751109	MS.gene002295:CDS
TCCGGTGGTGGTGGCCGTTA+CGG	0.303334	2.3:-72751110	MS.gene002295:CDS
TATTAATCATGCACTTAGTA+TGG	0.314323	2.3:+72750840	None:intergenic
AATAGTAACACATGTGCTTC+TGG	0.333085	2.3:-72751002	MS.gene002295:CDS
GCTGGGCTTATCCCTCATTA+TGG	0.334186	2.3:-72751294	MS.gene002295:CDS
CATAGTCTGCTTGTGAGTCT+TGG	0.335837	2.3:+72751255	None:intergenic
GCCATTATACTCAGGTGGTT+TGG	0.358578	2.3:-72750968	MS.gene002295:CDS
GTTACTATTATAGTTATAAT+CGG	0.390895	2.3:+72751016	None:intergenic
TCCAAACCACCTGAGTATAA+TGG	0.398812	2.3:+72750967	None:intergenic
ATAGTCTGCTTGTGAGTCTT+GGG	0.401577	2.3:+72751256	None:intergenic
TTGGTGTTGCAAATATTGTT+TGG	0.405178	2.3:-72751202	MS.gene002295:CDS
CCCGTAACGGCCACCACCAC+CGG	0.429882	2.3:+72751109	None:intergenic
TGGAGAAACACAAAGCGTAT+TGG	0.455942	2.3:-72750948	MS.gene002295:CDS
TGGTGTTGCAAATATTGTTT+GGG	0.459680	2.3:-72751201	MS.gene002295:CDS
GCACAGATGCAGTGAGGTTG+TGG	0.462999	2.3:-72751052	MS.gene002295:CDS
ATTATTATCACATTTCACTT+TGG	0.463790	2.3:+72750920	None:intergenic
CCACAACGATGCAAGGCGTC+AGG	0.478298	2.3:-72751225	MS.gene002295:CDS
TTGATCCACTCCGGTGGTGG+TGG	0.492178	2.3:-72751119	MS.gene002295:CDS
GTGAAATGTGATAATAATAG+AGG	0.492513	2.3:-72750915	MS.gene002295:CDS
CTGACGCCTTGCATCGTTGT+GGG	0.498716	2.3:+72751226	None:intergenic
GAGAATCTAGCGTGGAGCTC+CGG	0.500119	2.3:-72751086	MS.gene002295:CDS
ATCTGTGCCACTCATGTCGC+CGG	0.501762	2.3:+72751067	None:intergenic
CGATTGATCCACTCCGGTGG+TGG	0.538844	2.3:-72751122	MS.gene002295:CDS
CACAGATGCAGTGAGGTTGT+GGG	0.544377	2.3:-72751051	MS.gene002295:CDS
CCTGACGCCTTGCATCGTTG+TGG	0.564453	2.3:+72751225	None:intergenic
GTGTAACATGAACCATAATG+AGG	0.566235	2.3:+72751282	None:intergenic
CGGTGGTGGTGGCCGTTACG+GGG	0.566376	2.3:-72751108	MS.gene002295:CDS
TGAACGCCCACAACGATGCA+AGG	0.600263	2.3:-72751232	MS.gene002295:CDS
TGGAGCTCCGGCGACATGAG+TGG	0.603470	2.3:-72751074	MS.gene002295:CDS
AGACTATGCTAATCAACGCA+AGG	0.607234	2.3:-72751153	MS.gene002295:CDS
GATCCACCAGGCAACTATGT+TGG	0.607501	2.3:-72750870	MS.gene002295:CDS
AACGGCCACCACCACCGGAG+TGG	0.614459	2.3:+72751114	None:intergenic
TGACCAACATAGTTGCCTGG+TGG	0.614666	2.3:+72750867	None:intergenic
TGTAACATGAACCATAATGA+GGG	0.618907	2.3:+72751283	None:intergenic
AACGATGCAAGGCGTCAGGT+TGG	0.623173	2.3:-72751221	MS.gene002295:CDS
GACTATGCTAATCAACGCAA+GGG	0.626800	2.3:-72751152	MS.gene002295:CDS
GTGTGGCCATTATACTCAGG+TGG	0.627875	2.3:-72750973	MS.gene002295:CDS
GATTGTCGATTGATCCACTC+CGG	0.648727	2.3:-72751128	MS.gene002295:CDS
ACGCTAGATTCTCCCCGTAA+CGG	0.657514	2.3:+72751096	None:intergenic
ACTTGCAATTACGATCCACC+AGG	0.660677	2.3:-72750882	MS.gene002295:CDS
AGTGTGTGGCCATTATACTC+AGG	0.664484	2.3:-72750976	MS.gene002295:CDS
TGTGCTTCTGGTAAAGTGTG+TGG	0.690422	2.3:-72750990	MS.gene002295:CDS
GTTACGGGGAGAATCTAGCG+TGG	0.716019	2.3:-72751094	MS.gene002295:CDS
TGTCGATTGATCCACTCCGG+TGG	0.719544	2.3:-72751125	MS.gene002295:CDS
TGAGTGGCACAGATGCAGTG+AGG	0.786885	2.3:-72751058	MS.gene002295:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTACTATTATAGTTATAAT+CGG	+	chr2.3:72751161-72751180	None:intergenic	15.0%
!!	ATTATTATCACATTTCACTT+TGG	+	chr2.3:72751257-72751276	None:intergenic	20.0%
!	GTGAAATGTGATAATAATAG+AGG	-	chr2.3:72751259-72751278	MS.gene002295:CDS	25.0%
	TGTAACATGAACCATAATGA+GGG	+	chr2.3:72750894-72750913	None:intergenic	30.0%
!!	TGGTGTTGCAAATATTGTTT+GGG	-	chr2.3:72750973-72750992	MS.gene002295:CDS	30.0%
!!	TTGGTGTTGCAAATATTGTT+TGG	-	chr2.3:72750972-72750991	MS.gene002295:CDS	30.0%
	AATAGTAACACATGTGCTTC+TGG	-	chr2.3:72751172-72751191	MS.gene002295:CDS	35.0%
	GTGTAACATGAACCATAATG+AGG	+	chr2.3:72750895-72750914	None:intergenic	35.0%
!	ATTTTCTCTATTGTGTGTGC+TGG	-	chr2.3:72750862-72750881	MS.gene002295:CDS	35.0%
!	TTTTCTCTATTGTGTGTGCT+GGG	-	chr2.3:72750863-72750882	MS.gene002295:CDS	35.0%
	AGACTATGCTAATCAACGCA+AGG	-	chr2.3:72751021-72751040	MS.gene002295:CDS	40.0%
	ATAGTCTGCTTGTGAGTCTT+GGG	+	chr2.3:72750921-72750940	None:intergenic	40.0%
	GACTATGCTAATCAACGCAA+GGG	-	chr2.3:72751022-72751041	MS.gene002295:CDS	40.0%
	TCCAAACCACCTGAGTATAA+TGG	+	chr2.3:72751210-72751229	None:intergenic	40.0%
	TGGAGAAACACAAAGCGTAT+TGG	-	chr2.3:72751226-72751245	MS.gene002295:CDS	40.0%
!	TTTTGACCAACATAGTTGCC+TGG	+	chr2.3:72751313-72751332	None:intergenic	40.0%
	ACTTGCAATTACGATCCACC+AGG	-	chr2.3:72751292-72751311	MS.gene002295:CDS	45.0%
	AGTGTGTGGCCATTATACTC+AGG	-	chr2.3:72751198-72751217	MS.gene002295:CDS	45.0%
	CATAGTCTGCTTGTGAGTCT+TGG	+	chr2.3:72750922-72750941	None:intergenic	45.0%
	GCCATTATACTCAGGTGGTT+TGG	-	chr2.3:72751206-72751225	MS.gene002295:CDS	45.0%
	TGTGCTTCTGGTAAAGTGTG+TGG	-	chr2.3:72751184-72751203	MS.gene002295:CDS	45.0%
!	GATTGTCGATTGATCCACTC+CGG	-	chr2.3:72751046-72751065	MS.gene002295:CDS	45.0%
	ACGCTAGATTCTCCCCGTAA+CGG	+	chr2.3:72751081-72751100	None:intergenic	50.0%
	CACAGATGCAGTGAGGTTGT+GGG	-	chr2.3:72751123-72751142	MS.gene002295:CDS	50.0%
	GATCCACCAGGCAACTATGT+TGG	-	chr2.3:72751304-72751323	MS.gene002295:CDS	50.0%
	GCTGGGCTTATCCCTCATTA+TGG	-	chr2.3:72750880-72750899	MS.gene002295:CDS	50.0%
	GTGTGGCCATTATACTCAGG+TGG	-	chr2.3:72751201-72751220	MS.gene002295:CDS	50.0%
	TGACCAACATAGTTGCCTGG+TGG	+	chr2.3:72751310-72751329	None:intergenic	50.0%
	CTGACGCCTTGCATCGTTGT+GGG	+	chr2.3:72750951-72750970	None:intergenic	55.0%
	GAGAATCTAGCGTGGAGCTC+CGG	-	chr2.3:72751088-72751107	MS.gene002295:CDS	55.0%
	GCACAGATGCAGTGAGGTTG+TGG	-	chr2.3:72751122-72751141	MS.gene002295:CDS	55.0%
	GTTACGGGGAGAATCTAGCG+TGG	-	chr2.3:72751080-72751099	MS.gene002295:CDS	55.0%
	TGAACGCCCACAACGATGCA+AGG	-	chr2.3:72750942-72750961	MS.gene002295:CDS	55.0%
	TGAGTGGCACAGATGCAGTG+AGG	-	chr2.3:72751116-72751135	MS.gene002295:CDS	55.0%
!	AACGATGCAAGGCGTCAGGT+TGG	-	chr2.3:72750953-72750972	MS.gene002295:CDS	55.0%
!	ATCTGTGCCACTCATGTCGC+CGG	+	chr2.3:72751110-72751129	None:intergenic	55.0%
!	TGTCGATTGATCCACTCCGG+TGG	-	chr2.3:72751049-72751068	MS.gene002295:CDS	55.0%
	CCTGACGCCTTGCATCGTTG+TGG	+	chr2.3:72750952-72750971	None:intergenic	60.0%
	CGATTGATCCACTCCGGTGG+TGG	-	chr2.3:72751052-72751071	MS.gene002295:CDS	60.0%
!	CCACAACGATGCAAGGCGTC+AGG	-	chr2.3:72750949-72750968	MS.gene002295:CDS	60.0%
!	TTGATCCACTCCGGTGGTGG+TGG	-	chr2.3:72751055-72751074	MS.gene002295:CDS	60.0%
	TGGAGCTCCGGCGACATGAG+TGG	-	chr2.3:72751100-72751119	MS.gene002295:CDS	65.0%
!!	TCCGGTGGTGGTGGCCGTTA+CGG	-	chr2.3:72751064-72751083	MS.gene002295:CDS	65.0%
	AACGGCCACCACCACCGGAG+TGG	+	chr2.3:72751063-72751082	None:intergenic	70.0%
	CCCGTAACGGCCACCACCAC+CGG	+	chr2.3:72751068-72751087	None:intergenic	70.0%
!!	CCGGTGGTGGTGGCCGTTAC+GGG	-	chr2.3:72751065-72751084	MS.gene002295:CDS	70.0%
!!	CGGTGGTGGTGGCCGTTACG+GGG	-	chr2.3:72751066-72751085	MS.gene002295:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	72750854	72751342	72750854	ID=MS.gene002295
chr2.3	mRNA	72750854	72751342	72750854	ID=MS.gene002295.t1;Parent=MS.gene002295
chr2.3	exon	72750854	72751342	72750854	ID=MS.gene002295.t1.exon1;Parent=MS.gene002295.t1
chr2.3	CDS	72750854	72751342	72750854	ID=cds.MS.gene002295.t1;Parent=MS.gene002295.t1

Gene Sequence

>MS.gene002295

ATGAGTTCATTTTCTCTATTGTGTGTGCTGGGCTTATCCCTCATTATGGTTCATGTTACACATGCCCAAGACTCACAAGCAGACTATGTGAACGCCCACAACGATGCAAGGCGTCAGGTTGGTGTTGCAAATATTGTTTGGGACAATAATGTTGCTGCTTTTGCACAAGACTATGCTAATCAACGCAAGGGTGATTGTCGATTGATCCACTCCGGTGGTGGTGGCCGTTACGGGGAGAATCTAGCGTGGAGCTCCGGCGACATGAGTGGCACAGATGCAGTGAGGTTGTGGGTGAATGAGAGAGCCGATTATAACTATAATAGTAACACATGTGCTTCTGGTAAAGTGTGTGGCCATTATACTCAGGTGGTTTGGAGAAACACAAAGCGTATTGGATGTGCCAAAGTGAAATGTGATAATAATAGAGGAACATTCATTACTTGCAATTACGATCCACCAGGCAACTATGTTGGTCAAAAGCCATACTAA

Protein sequence

>MS.gene002295.t1

MSSFSLLCVLGLSLIMVHVTHAQDSQADYVNAHNDARRQVGVANIVWDNNVAAFAQDYANQRKGDCRLIHSGGGGRYGENLAWSSGDMSGTDAVRLWVNERADYNYNSNTCASGKVCGHYTQVVWRNTKRIGCAKVKCDNNRGTFITCNYDPPGNYVGQKPY