AlfalfaGEDB | MS.gene002297

M. sativa cultivar XinJiangDaYe / MS.gene002297

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002297.t1	XP_003593361.1	98	150	3	0	1	150	16	165	1.10E-84	322.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002297.t1	P07053	58.7	150	54	4	3	150	25	168	8.4e-49	194.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002297.t1	G7IFD4	98.0	150	3	0	1	150	16	165	8.0e-85	322.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene002297	MS.gene002296	PPI
MS.gene01688	MS.gene002297	PPI
MS.gene01356	MS.gene002297	PPI
MS.gene01273	MS.gene002297	PPI
MS.gene002292	MS.gene002297	PPI
MS.gene002294	MS.gene002297	PPI
MS.gene012420	MS.gene002297	PPI
MS.gene002297	MS.gene01274	PPI
MS.gene002295	MS.gene002297	PPI
MS.gene02252	MS.gene002297	PPI
MS.gene002297	MS.gene02258	PPI
MS.gene038936	MS.gene002297	PPI
MS.gene035685	MS.gene002297	PPI
MS.gene01685	MS.gene002297	PPI
MS.gene02249	MS.gene002297	PPI
MS.gene01275	MS.gene002297	PPI
MS.gene028545	MS.gene002297	PPI
MS.gene01270	MS.gene002297	PPI
MS.gene01687	MS.gene002297	PPI
MS.gene01277	MS.gene002297	PPI
MS.gene035687	MS.gene002297	PPI
MS.gene002297	MS.gene038936	PPI
MS.gene01690	MS.gene002297	PPI
MS.gene01684	MS.gene002297	PPI
MS.gene02258	MS.gene002297	PPI
MS.gene00249	MS.gene002297	PPI
MS.gene002297	MS.gene002293	PPI
MS.gene02254	MS.gene002297	PPI
MS.gene018353	MS.gene002297	PPI
MS.gene002299	MS.gene002297	PPI
MS.gene01686	MS.gene002297	PPI
MS.gene02255	MS.gene002297	PPI
MS.gene57900	MS.gene002297	PPI
MS.gene01272	MS.gene002297	PPI
MS.gene02256	MS.gene002297	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002297.t1	MTR_2g010630	98.000	150	3	0	1	150	16	165	8.40e-111	311
MS.gene002297.t1	MTR_2g010650	89.189	148	14	2	3	150	20	165	4.26e-95	272
MS.gene002297.t1	MTR_2g010700	72.078	154	38	2	1	150	16	168	7.80e-80	233
MS.gene002297.t1	MTR_2g010600	74.497	149	32	3	4	150	18	162	1.10e-79	233
MS.gene002297.t1	MTR_4g050762	73.154	149	34	3	4	150	18	162	4.36e-77	227
MS.gene002297.t1	MTR_2g012370	73.154	149	34	3	4	150	18	162	4.36e-77	227
MS.gene002297.t1	MTR_2g010640	69.333	150	44	2	1	150	16	163	3.78e-73	216
MS.gene002297.t1	MTR_2g010670	69.333	150	45	1	1	150	16	164	5.14e-73	216
MS.gene002297.t1	MTR_2g010590	68.243	148	40	3	4	150	18	159	1.66e-72	214
MS.gene002297.t1	MTR_2g010690	66.667	150	49	1	1	150	16	164	4.39e-71	211
MS.gene002297.t1	MTR_2g010610	63.514	148	26	4	4	150	18	138	2.10e-59	181
MS.gene002297.t1	MTR_2g435490	56.667	150	57	4	3	150	21	164	3.36e-57	176
MS.gene002297.t1	MTR_2g010580	51.351	148	64	4	4	150	24	164	1.79e-50	159
MS.gene002297.t1	MTR_4g092540	48.026	152	70	5	1	150	15	159	3.58e-46	148
MS.gene002297.t1	MTR_4g128750	55.396	139	53	5	15	150	41	173	1.87e-44	144
MS.gene002297.t1	MTR_4g128770	48.936	141	64	5	14	150	70	206	2.52e-44	144
MS.gene002297.t1	MTR_1g067290	39.007	141	75	6	14	149	73	207	6.72e-28	103
MS.gene002297.t1	MTR_8g078770	34.507	142	83	5	14	150	36	172	1.23e-23	90.9
MS.gene002297.t1	MTR_5g018770	31.944	144	86	5	14	150	47	185	4.30e-21	84.7
MS.gene002297.t1	MTR_4g092515	53.165	79	29	4	6	82	24	96	1.58e-17	74.3
MS.gene002297.t1	MTR_5g018755	35.484	93	53	3	62	150	14	103	2.23e-13	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002297.t1	AT4G33720	62.500	144	47	3	8	150	26	163	8.65e-61	185
MS.gene002297.t1	AT5G26130	59.722	144	53	2	8	150	27	166	9.57e-58	177
MS.gene002297.t1	AT2G14580	62.500	144	45	5	8	150	26	161	7.61e-57	175
MS.gene002297.t1	AT4G33710	58.333	144	55	2	8	150	27	166	7.90e-57	175
MS.gene002297.t1	AT3G19690	58.219	146	52	4	8	150	22	161	3.59e-56	173
MS.gene002297.t1	AT2G14610	57.534	146	53	5	6	150	24	161	2.67e-54	168
MS.gene002297.t1	AT1G50060	55.102	147	58	4	6	150	21	161	4.22e-51	160
MS.gene002297.t1	AT4G33730	51.333	150	64	4	3	150	30	172	6.07e-48	153
MS.gene002297.t1	AT4G25780	50.355	141	62	4	14	150	54	190	2.01e-45	147
MS.gene002297.t1	AT4G25790	49.286	140	62	5	14	150	77	210	1.26e-43	143
MS.gene002297.t1	AT1G50050	53.425	146	61	4	7	150	22	162	4.71e-43	140
MS.gene002297.t1	AT5G57625	49.640	139	61	5	15	150	75	207	1.69e-42	140
MS.gene002297.t1	AT4G30320	48.936	141	66	5	13	150	24	161	6.59e-41	134
MS.gene002297.t1	AT2G19990	46.809	141	67	4	12	150	42	176	1.88e-40	134
MS.gene002297.t1	AT3G09590	47.518	141	64	5	14	150	52	186	5.09e-40	133
MS.gene002297.t1	AT1G50050	52.899	138	58	4	7	142	22	154	9.87e-39	131
MS.gene002297.t1	AT4G07820	43.750	144	74	3	7	150	24	160	8.94e-38	126
MS.gene002297.t1	AT1G01310	46.043	139	67	4	14	150	87	219	2.81e-37	127
MS.gene002297.t1	AT5G02730	46.479	142	66	5	13	150	58	193	6.89e-37	125
MS.gene002297.t1	AT4G31470	42.857	140	70	6	14	150	53	185	9.34e-35	119
MS.gene002297.t1	AT5G66590	40.141	142	74	5	15	150	49	185	9.68e-31	109
MS.gene002297.t1	AT2G19980	41.781	146	63	7	12	150	35	165	7.91e-26	96.3
MS.gene002297.t1	AT2G19970	37.086	151	75	7	12	150	35	177	1.76e-21	85.5

Find 42 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTTATCCCAAACAATATT+TGG	0.174562	2.3:+72743749	None:intergenic
TTAAAATTCCAAATATTGTT+TGG	0.286243	2.3:-72743757	MS.gene002297:CDS
TAAAATTCCAAATATTGTTT+GGG	0.343829	2.3:-72743756	MS.gene002297:CDS
TTGGTCCACTCCGGTGGTGG+TGG	0.369000	2.3:-72743677	MS.gene002297:CDS
ATTACTACTATAATCATAGT+AGG	0.395772	2.3:+72743574	None:intergenic
GCCACTATACTCAGGTGGTT+TGG	0.400142	2.3:-72743526	MS.gene002297:CDS
CTTTCGCCAATATAGTTGCC+TGG	0.409431	2.3:+72743425	None:intergenic
TCCGGTGGTGGTGGGCGTTA+CGG	0.427305	2.3:-72743668	MS.gene002297:CDS
AGTAGTAATTCATGCGCTAA+TGG	0.432705	2.3:-72743560	MS.gene002297:CDS
GCCAAAGTGAAATGTGATAA+TGG	0.434324	2.3:-72743479	MS.gene002297:CDS
TTTCGCCAATATAGTTGCCT+GGG	0.464440	2.3:+72743426	None:intergenic
ACCATTATCACATTTCACTT+TGG	0.465618	2.3:+72743478	None:intergenic
GAGAATATCGCGGAGAGCAC+TGG	0.467165	2.3:-72743644	MS.gene002297:CDS
CGCCCACAACAATGCAAGAT+CGG	0.477733	2.3:-72743795	MS.gene002297:CDS
TGCCGATCTTGCATTGTTGT+GGG	0.478465	2.3:+72743793	None:intergenic
TCCGTAACGCCCACCACCAC+CGG	0.482574	2.3:+72743667	None:intergenic
TCAACGCAAGGATTGTCAAT+TGG	0.501362	2.3:-72743696	MS.gene002297:CDS
TGGTCCACTCCGGTGGTGGT+GGG	0.501538	2.3:-72743676	MS.gene002297:CDS
CTGCCGATCTTGCATTGTTG+TGG	0.503037	2.3:+72743792	None:intergenic
TGGGCGTTCACGTAGTCTGC+TGG	0.508423	2.3:+72743812	None:intergenic
TCCAAACCACCTGAGTATAG+TGG	0.511835	2.3:+72743525	None:intergenic
GCGTAGAAGCGGTGAAGTTG+TGG	0.525292	2.3:-72743610	MS.gene002297:CDS
TGGAGAAACACACAACGGAT+TGG	0.526081	2.3:-72743506	MS.gene002297:CDS
CGTAGAAGCGGTGAAGTTGT+GGG	0.531099	2.3:-72743609	MS.gene002297:CDS
CAATTGGTCCACTCCGGTGG+TGG	0.548354	2.3:-72743680	MS.gene002297:CDS
TTACTACTATAATCATAGTA+GGG	0.560092	2.3:+72743575	None:intergenic
CGCCAATATAGTTGCCTGGG+GGG	0.569732	2.3:+72743429	None:intergenic
TTCGCCAATATAGTTGCCTG+GGG	0.581612	2.3:+72743427	None:intergenic
CGACATGAGTGGCGTAGAAG+CGG	0.591511	2.3:-72743621	MS.gene002297:CDS
GAGAGCACTGGCGACATGAG+TGG	0.598119	2.3:-72743632	MS.gene002297:CDS
TGTCAATTGGTCCACTCCGG+TGG	0.599606	2.3:-72743683	MS.gene002297:CDS
AACGCCCACCACCACCGGAG+TGG	0.599839	2.3:+72743672	None:intergenic
AAACTATGCTAATCAACGCA+AGG	0.625421	2.3:-72743708	MS.gene002297:CDS
GATTGTCAATTGGTCCACTC+CGG	0.637187	2.3:-72743686	MS.gene002297:CDS
TCGCCAATATAGTTGCCTGG+GGG	0.640565	2.3:+72743428	None:intergenic
GTGTGGCCACTATACTCAGG+TGG	0.688606	2.3:-72743531	MS.gene002297:CDS
AGAAGCGGTGAAGTTGTGGG+TGG	0.698947	2.3:-72743606	MS.gene002297:CDS
TGCGCTAATGGTGAAATGTG+TGG	0.704274	2.3:-72743548	MS.gene002297:CDS
AATGTGTGGCCACTATACTC+AGG	0.735120	2.3:-72743534	MS.gene002297:CDS
TGGTTTGGAGAAACACACAA+CGG	0.760577	2.3:-72743511	MS.gene002297:CDS
AAAGTGAAATGTGATAATGG+TGG	0.767769	2.3:-72743476	MS.gene002297:CDS
GCGTTACGGAGAGAATATCG+CGG	0.795262	2.3:-72743654	MS.gene002297:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATTCCAAATATTGTTT+GGG	-	chr2.3:72743504-72743523	MS.gene002297:CDS	15.0%
!!	TTAAAATTCCAAATATTGTT+TGG	-	chr2.3:72743503-72743522	MS.gene002297:CDS	15.0%
!!	ATTACTACTATAATCATAGT+AGG	+	chr2.3:72743689-72743708	None:intergenic	20.0%
!!	TTACTACTATAATCATAGTA+GGG	+	chr2.3:72743688-72743707	None:intergenic	20.0%
!	TTGTTATCCCAAACAATATT+TGG	+	chr2.3:72743514-72743533	None:intergenic	25.0%
	ACCATTATCACATTTCACTT+TGG	+	chr2.3:72743785-72743804	None:intergenic	30.0%
!!	AAAGTGAAATGTGATAATGG+TGG	-	chr2.3:72743784-72743803	MS.gene002297:CDS	30.0%
	AAACTATGCTAATCAACGCA+AGG	-	chr2.3:72743552-72743571	MS.gene002297:CDS	35.0%
	AGTAGTAATTCATGCGCTAA+TGG	-	chr2.3:72743700-72743719	MS.gene002297:CDS	35.0%
	GCCAAAGTGAAATGTGATAA+TGG	-	chr2.3:72743781-72743800	MS.gene002297:CDS	35.0%
	TCAACGCAAGGATTGTCAAT+TGG	-	chr2.3:72743564-72743583	MS.gene002297:CDS	40.0%
	TTTCGCCAATATAGTTGCCT+GGG	+	chr2.3:72743837-72743856	None:intergenic	40.0%
!	TGGTTTGGAGAAACACACAA+CGG	-	chr2.3:72743749-72743768	MS.gene002297:CDS	40.0%
	AATGTGTGGCCACTATACTC+AGG	-	chr2.3:72743726-72743745	MS.gene002297:CDS	45.0%
	CTTTCGCCAATATAGTTGCC+TGG	+	chr2.3:72743838-72743857	None:intergenic	45.0%
	GATTGTCAATTGGTCCACTC+CGG	-	chr2.3:72743574-72743593	MS.gene002297:CDS	45.0%
	TCCAAACCACCTGAGTATAG+TGG	+	chr2.3:72743738-72743757	None:intergenic	45.0%
	TGCCGATCTTGCATTGTTGT+GGG	+	chr2.3:72743470-72743489	None:intergenic	45.0%
	TGCGCTAATGGTGAAATGTG+TGG	-	chr2.3:72743712-72743731	MS.gene002297:CDS	45.0%
	TGGAGAAACACACAACGGAT+TGG	-	chr2.3:72743754-72743773	MS.gene002297:CDS	45.0%
	TTCGCCAATATAGTTGCCTG+GGG	+	chr2.3:72743836-72743855	None:intergenic	45.0%
	CGCCCACAACAATGCAAGAT+CGG	-	chr2.3:72743465-72743484	MS.gene002297:CDS	50.0%
	CGTAGAAGCGGTGAAGTTGT+GGG	-	chr2.3:72743651-72743670	MS.gene002297:CDS	50.0%
	CTGCCGATCTTGCATTGTTG+TGG	+	chr2.3:72743471-72743490	None:intergenic	50.0%
	GCCACTATACTCAGGTGGTT+TGG	-	chr2.3:72743734-72743753	MS.gene002297:CDS	50.0%
	GCGTTACGGAGAGAATATCG+CGG	-	chr2.3:72743606-72743625	MS.gene002297:CDS	50.0%
	TCGCCAATATAGTTGCCTGG+GGG	+	chr2.3:72743835-72743854	None:intergenic	50.0%
	ACTTGCAATTACGACCCCCC+AGG	-	chr2.3:72743817-72743836	MS.gene002297:CDS	55.0%
	AGAAGCGGTGAAGTTGTGGG+TGG	-	chr2.3:72743654-72743673	MS.gene002297:CDS	55.0%
	CGACATGAGTGGCGTAGAAG+CGG	-	chr2.3:72743639-72743658	MS.gene002297:CDS	55.0%
	CGCCAATATAGTTGCCTGGG+GGG	+	chr2.3:72743834-72743853	None:intergenic	55.0%
	GACCCCCCAGGCAACTATAT+TGG	-	chr2.3:72743829-72743848	MS.gene002297:CDS	55.0%
	GCGTAGAAGCGGTGAAGTTG+TGG	-	chr2.3:72743650-72743669	MS.gene002297:CDS	55.0%
	GTGTGGCCACTATACTCAGG+TGG	-	chr2.3:72743729-72743748	MS.gene002297:CDS	55.0%
	TGTCAATTGGTCCACTCCGG+TGG	-	chr2.3:72743577-72743596	MS.gene002297:CDS	55.0%
!	GAGAATATCGCGGAGAGCAC+TGG	-	chr2.3:72743616-72743635	MS.gene002297:CDS	55.0%
	CAATTGGTCCACTCCGGTGG+TGG	-	chr2.3:72743580-72743599	MS.gene002297:CDS	60.0%
	TGGGCGTTCACGTAGTCTGC+TGG	+	chr2.3:72743451-72743470	None:intergenic	60.0%
!!	GAGAGCACTGGCGACATGAG+TGG	-	chr2.3:72743628-72743647	MS.gene002297:CDS	60.0%
	TCCGTAACGCCCACCACCAC+CGG	+	chr2.3:72743596-72743615	None:intergenic	65.0%
!	TGGTCCACTCCGGTGGTGGT+GGG	-	chr2.3:72743584-72743603	MS.gene002297:CDS	65.0%
!	TTGGTCCACTCCGGTGGTGG+TGG	-	chr2.3:72743583-72743602	MS.gene002297:CDS	65.0%
!!	TCCGGTGGTGGTGGGCGTTA+CGG	-	chr2.3:72743592-72743611	MS.gene002297:CDS	65.0%
	AACGCCCACCACCACCGGAG+TGG	+	chr2.3:72743591-72743610	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	72743415	72743867	72743415	ID=MS.gene002297
chr2.3	mRNA	72743415	72743867	72743415	ID=MS.gene002297.t1;Parent=MS.gene002297
chr2.3	exon	72743415	72743867	72743415	ID=MS.gene002297.t1.exon1;Parent=MS.gene002297.t1
chr2.3	CDS	72743415	72743867	72743415	ID=cds.MS.gene002297.t1;Parent=MS.gene002297.t1

Gene Sequence

>MS.gene002297

ATGGGTAGTCACGTTGCACATGCACAAGACTCACCAGCAGACTACGTGAACGCCCACAACAATGCAAGATCGGCAGTTCATACAAACGTTAAAATTCCAAATATTGTTTGGGATAACAAAGTTGCTGCTTTTGCACAAAACTATGCTAATCAACGCAAGGATTGTCAATTGGTCCACTCCGGTGGTGGTGGGCGTTACGGAGAGAATATCGCGGAGAGCACTGGCGACATGAGTGGCGTAGAAGCGGTGAAGTTGTGGGTGGATGAAAAACCCTACTATGATTATAGTAGTAATTCATGCGCTAATGGTGAAATGTGTGGCCACTATACTCAGGTGGTTTGGAGAAACACACAACGGATTGGATGTGCCAAAGTGAAATGTGATAATGGTGGCACATTCATTACTTGCAATTACGACCCCCCAGGCAACTATATTGGCGAAAGACCTTACTAA

Protein sequence

>MS.gene002297.t1

MGSHVAHAQDSPADYVNAHNNARSAVHTNVKIPNIVWDNKVAAFAQNYANQRKDCQLVHSGGGGRYGENIAESTGDMSGVEAVKLWVDEKPYYDYSSNSCANGEMCGHYTQVVWRNTQRIGCAKVKCDNGGTFITCNYDPPGNYIGERPY