AlfalfaGEDB | MS.gene01687

M. sativa cultivar XinJiangDaYe / MS.gene01687

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01687.t1	RHN71757.1	85.8	162	20	2	1	159	274	435	2.50E-74	288.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01687.t1	Q9ZNS4	65.0	157	50	2	3	159	10	161	1.4e-54	213.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01687.t1	G7IFD1	85.8	162	20	2	1	159	1	162	1.8e-74	288.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene01687	MS.gene01684	PPI
MS.gene01687	MS.gene038936	PPI
MS.gene01687	MS.gene012420	PPI
MS.gene01687	MS.gene00249	PPI
MS.gene01687	MS.gene035685	PPI
MS.gene01687	MS.gene02252	PPI
MS.gene01687	MS.gene002293	PPI
MS.gene01687	MS.gene01270	PPI
MS.gene01687	MS.gene02249	PPI
MS.gene002299	MS.gene01687	PPI
MS.gene01687	MS.gene57900	PPI
MS.gene01688	MS.gene01687	PPI
MS.gene01687	MS.gene002299	PPI
MS.gene01687	MS.gene002296	PPI
MS.gene01687	MS.gene01272	PPI
MS.gene01687	MS.gene002297	PPI
MS.gene01273	MS.gene01687	PPI
MS.gene01687	MS.gene01277	PPI
MS.gene01687	MS.gene01688	PPI
MS.gene01687	MS.gene02256	PPI
MS.gene01687	MS.gene01690	PPI
MS.gene01687	MS.gene02255	PPI
MS.gene01687	MS.gene01686	PPI
MS.gene01687	MS.gene018353	PPI
MS.gene01687	MS.gene002295	PPI
MS.gene01687	MS.gene002294	PPI
MS.gene01687	MS.gene01274	PPI
MS.gene01687	MS.gene01275	PPI
MS.gene01687	MS.gene01356	PPI
MS.gene01687	MS.gene002292	PPI
MS.gene01687	MS.gene02254	PPI
MS.gene01687	MS.gene01685	PPI
MS.gene01687	MS.gene035687	PPI
MS.gene028545	MS.gene01687	PPI
MS.gene01687	MS.gene01273	PPI
MS.gene01687	MS.gene02258	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01687.t1	MTR_2g010600	85.802	162	20	2	1	159	1	162	1.15e-98	281
MS.gene01687.t1	MTR_2g010590	78.616	159	34	0	1	159	1	159	2.19e-96	275
MS.gene01687.t1	MTR_4g050762	84.568	162	22	2	1	159	1	162	3.73e-90	260
MS.gene01687.t1	MTR_2g012370	84.568	162	22	2	1	159	1	162	3.73e-90	260
MS.gene01687.t1	MTR_2g010610	81.250	160	7	3	1	159	1	138	1.42e-78	229
MS.gene01687.t1	MTR_2g010650	68.263	167	43	6	1	159	1	165	1.11e-76	226
MS.gene01687.t1	MTR_2g010630	70.588	153	38	3	13	159	14	165	2.09e-75	222
MS.gene01687.t1	MTR_2g010700	60.355	169	56	5	1	159	1	168	4.59e-65	196
MS.gene01687.t1	MTR_2g010640	61.212	165	56	5	1	159	1	163	1.45e-63	192
MS.gene01687.t1	MTR_2g435490	54.487	156	69	2	6	159	9	164	1.37e-60	186
MS.gene01687.t1	MTR_2g010670	59.036	166	59	5	1	159	1	164	1.24e-58	180
MS.gene01687.t1	MTR_2g010690	56.627	166	63	5	1	159	1	164	4.11e-57	176
MS.gene01687.t1	MTR_2g010580	47.170	159	83	1	1	159	7	164	4.25e-54	168
MS.gene01687.t1	MTR_4g092540	49.375	160	70	3	1	159	10	159	2.24e-52	164
MS.gene01687.t1	MTR_4g128750	51.128	133	63	2	29	159	41	173	1.48e-42	139
MS.gene01687.t1	MTR_4g128770	44.526	137	71	2	28	159	70	206	3.24e-40	134
MS.gene01687.t1	MTR_1g067290	42.963	135	73	3	28	158	73	207	3.76e-34	119
MS.gene01687.t1	MTR_8g078770	35.329	167	94	5	7	159	6	172	7.59e-27	99.8
MS.gene01687.t1	MTR_4g092515	51.961	102	46	2	1	101	7	106	1.78e-26	97.4
MS.gene01687.t1	MTR_5g018770	34.270	178	95	6	3	159	9	185	2.18e-25	96.3
MS.gene01687.t1	MTR_5g018755	35.789	95	55	4	71	159	9	103	4.37e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01687.t1	AT2G14580	64.968	157	50	2	3	159	10	161	2.26e-70	209
MS.gene01687.t1	AT4G33720	61.146	157	58	2	3	159	10	163	3.00e-67	202
MS.gene01687.t1	AT2G14610	60.390	154	59	1	6	159	10	161	7.86e-64	193
MS.gene01687.t1	AT3G19690	55.828	163	66	3	1	159	1	161	2.19e-62	189
MS.gene01687.t1	AT5G26130	56.024	166	66	2	1	159	1	166	3.18e-61	186
MS.gene01687.t1	AT4G33710	60.000	140	54	1	22	159	27	166	4.84e-58	178
MS.gene01687.t1	AT1G50060	50.318	157	74	2	4	159	8	161	1.55e-50	159
MS.gene01687.t1	AT4G33730	54.135	133	59	2	28	159	41	172	2.55e-47	151
MS.gene01687.t1	AT2G19990	53.906	128	58	1	33	159	49	176	3.08e-47	151
MS.gene01687.t1	AT4G25790	51.471	136	64	2	26	159	75	210	1.50e-46	151
MS.gene01687.t1	AT5G57625	51.880	133	62	2	29	159	75	207	1.22e-45	148
MS.gene01687.t1	AT1G50050	50.000	158	74	3	4	159	8	162	6.66e-45	145
MS.gene01687.t1	AT3G09590	47.407	135	68	2	28	159	52	186	1.92e-43	142
MS.gene01687.t1	AT4G30320	44.966	149	79	3	13	159	14	161	2.28e-43	141
MS.gene01687.t1	AT4G25780	48.905	137	65	3	28	159	54	190	1.14e-42	140
MS.gene01687.t1	AT1G50050	48.718	156	75	3	4	157	8	160	7.38e-42	139
MS.gene01687.t1	AT4G07820	46.763	139	72	2	21	159	24	160	5.43e-41	135
MS.gene01687.t1	AT1G01310	47.015	134	68	2	28	159	87	219	2.07e-40	136
MS.gene01687.t1	AT5G02730	44.853	136	72	2	27	159	58	193	9.05e-40	133
MS.gene01687.t1	AT4G31470	43.704	135	75	1	26	159	51	185	7.37e-38	128
MS.gene01687.t1	AT5G66590	42.336	137	73	4	29	159	49	185	3.23e-31	111
MS.gene01687.t1	AT2G19970	43.262	141	67	7	30	159	39	177	1.06e-27	102
MS.gene01687.t1	AT2G19980	36.905	168	84	9	4	159	8	165	4.94e-23	89.7

Find 39 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCACTCCGGTGGCCGTTAC+GGG	0.273758	2.4:-72707806	MS.gene01687:CDS
TTGGGCTTATTACTCATTAT+TGG	0.280398	2.4:-72707984	MS.gene01687:CDS
ATCCACTCCGGTGGCCGTTA+CGG	0.326170	2.4:-72707807	MS.gene01687:CDS
CATAGTCTGCTTGTGAGTCT+TGG	0.335837	2.4:+72707946	None:intergenic
TTGGTGTTCAAAATATTGTT+TGG	0.341386	2.4:-72707893	MS.gene01687:CDS
AATAGTAACACATGTGCTTC+TGG	0.347660	2.4:-72707699	MS.gene01687:CDS
TATTAAGCAAGCACTTAGTA+TGG	0.369831	2.4:+72707540	None:intergenic
ATCTGAGCCACTCAAGTCTC+CGG	0.382702	2.4:+72707764	None:intergenic
TCCATACAACCTGAGTATAA+TGG	0.399517	2.4:+72707664	None:intergenic
TGGTGTTCAAAATATTGTTT+GGG	0.400203	2.4:-72707892	MS.gene01687:CDS
ATAGTCTGCTTGTGAGTCTT+GGG	0.401577	2.4:+72707947	None:intergenic
GCCATTATACTCAGGTTGTA+TGG	0.418188	2.4:-72707665	MS.gene01687:CDS
TCTGAGCCACTCAAGTCTCC+GGG	0.459067	2.4:+72707765	None:intergenic
CGTTATTATAGTTGCCTGGT+GGG	0.465282	2.4:+72707568	None:intergenic
GCCAAAGTGAGATGTGATAA+TGG	0.484296	2.4:-72707618	MS.gene01687:CDS
TCCATTATCACATCTCACTT+TGG	0.498337	2.4:+72707617	None:intergenic
CCCCGTAACGGCCACCGGAG+TGG	0.509997	2.4:+72707805	None:intergenic
GAGAATCTCGCGGGTAGCCC+CGG	0.516219	2.4:-72707783	MS.gene01687:CDS
TGGAGAAACTCACAACGTCT+TGG	0.524024	2.4:-72707645	MS.gene01687:CDS
ATTCTCCCCGTAACGGCCAC+CGG	0.528380	2.4:+72707800	None:intergenic
GCTCAGATGCAGTGAAGTTG+TGG	0.543630	2.4:-72707749	MS.gene01687:CDS
CTCAGATGCAGTGAAGTTGT+GGG	0.551141	2.4:-72707748	MS.gene01687:CDS
TCGTTATTATAGTTGCCTGG+TGG	0.573306	2.4:+72707567	None:intergenic
ACTTGCAATTACGACCCACC+AGG	0.586857	2.4:-72707582	MS.gene01687:CDS
CCACTCCGGTGGCCGTTACG+GGG	0.597515	2.4:-72707805	MS.gene01687:CDS
CCGCGAGATTCTCCCCGTAA+CGG	0.609234	2.4:+72707793	None:intergenic
CCGTTACGGGGAGAATCTCG+CGG	0.614640	2.4:-72707793	MS.gene01687:CDS
CGTTACGGGGAGAATCTCGC+GGG	0.631764	2.4:-72707792	MS.gene01687:CDS
AACGAAGCAAGATCTGAGGT+TGG	0.637008	2.4:-72707912	MS.gene01687:CDS
AGACTATGCTAATCAACACA+AGG	0.643418	2.4:-72707844	MS.gene01687:CDS
AGTGTGTGGCCATTATACTC+AGG	0.651003	2.4:-72707673	MS.gene01687:CDS
GGTAGCCCCGGAGACTTGAG+TGG	0.652738	2.4:-72707771	MS.gene01687:CDS
TGTCAACTGATCCACTCCGG+TGG	0.664496	2.4:-72707816	MS.gene01687:CDS
GATTGTCAACTGATCCACTC+CGG	0.665515	2.4:-72707819	MS.gene01687:CDS
GACTATGCTAATCAACACAA+GGG	0.683970	2.4:-72707843	MS.gene01687:CDS
TGTGCTTCTGGTAAAGTGTG+TGG	0.690422	2.4:-72707687	MS.gene01687:CDS
TCACAACGAAGCAAGATCTG+AGG	0.699112	2.4:-72707916	MS.gene01687:CDS
CTGAGCCACTCAAGTCTCCG+GGG	0.708120	2.4:+72707766	None:intergenic
AAAGTGAGATGTGATAATGG+AGG	0.800107	2.4:-72707615	MS.gene01687:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGGTGTTCAAAATATTGTTT+GGG	-	chr2.4:72707673-72707692	MS.gene01687:CDS	25.0%
!!	ATTTTCTCTATTGTGTTTCT+TGG	-	chr2.4:72707562-72707581	MS.gene01687:CDS	25.0%
!!	TTGGTGTTCAAAATATTGTT+TGG	-	chr2.4:72707672-72707691	MS.gene01687:CDS	25.0%
!!	TTTTCTCTATTGTGTTTCTT+GGG	-	chr2.4:72707563-72707582	MS.gene01687:CDS	25.0%
	TTGGGCTTATTACTCATTAT+TGG	-	chr2.4:72707581-72707600	MS.gene01687:CDS	30.0%
!	TTTTCGTTATTATAGTTGCC+TGG	+	chr2.4:72708004-72708023	None:intergenic	30.0%
	AATAGTAACACATGTGCTTC+TGG	-	chr2.4:72707866-72707885	MS.gene01687:CDS	35.0%
	AGACTATGCTAATCAACACA+AGG	-	chr2.4:72707721-72707740	MS.gene01687:CDS	35.0%
	GACTATGCTAATCAACACAA+GGG	-	chr2.4:72707722-72707741	MS.gene01687:CDS	35.0%
	TCCATACAACCTGAGTATAA+TGG	+	chr2.4:72707904-72707923	None:intergenic	35.0%
	TCCATTATCACATCTCACTT+TGG	+	chr2.4:72707951-72707970	None:intergenic	35.0%
!!	AAAGTGAGATGTGATAATGG+AGG	-	chr2.4:72707950-72707969	MS.gene01687:CDS	35.0%
	ATAGTCTGCTTGTGAGTCTT+GGG	+	chr2.4:72707621-72707640	None:intergenic	40.0%
	CGTTATTATAGTTGCCTGGT+GGG	+	chr2.4:72708000-72708019	None:intergenic	40.0%
	GCCAAAGTGAGATGTGATAA+TGG	-	chr2.4:72707947-72707966	MS.gene01687:CDS	40.0%
	TCGTTATTATAGTTGCCTGG+TGG	+	chr2.4:72708001-72708020	None:intergenic	40.0%
!	GCCATTATACTCAGGTTGTA+TGG	-	chr2.4:72707900-72707919	MS.gene01687:CDS	40.0%
	AGTGTGTGGCCATTATACTC+AGG	-	chr2.4:72707892-72707911	MS.gene01687:CDS	45.0%
	CATAGTCTGCTTGTGAGTCT+TGG	+	chr2.4:72707622-72707641	None:intergenic	45.0%
	CTCAGATGCAGTGAAGTTGT+GGG	-	chr2.4:72707817-72707836	MS.gene01687:CDS	45.0%
	GATTGTCAACTGATCCACTC+CGG	-	chr2.4:72707746-72707765	MS.gene01687:CDS	45.0%
	TCACAACGAAGCAAGATCTG+AGG	-	chr2.4:72707649-72707668	MS.gene01687:CDS	45.0%
	TGGAGAAACTCACAACGTCT+TGG	-	chr2.4:72707920-72707939	MS.gene01687:CDS	45.0%
	TGTGCTTCTGGTAAAGTGTG+TGG	-	chr2.4:72707878-72707897	MS.gene01687:CDS	45.0%
!	AACGAAGCAAGATCTGAGGT+TGG	-	chr2.4:72707653-72707672	MS.gene01687:CDS	45.0%
	ACTTGCAATTACGACCCACC+AGG	-	chr2.4:72707983-72708002	MS.gene01687:CDS	50.0%
	ATCTGAGCCACTCAAGTCTC+CGG	+	chr2.4:72707804-72707823	None:intergenic	50.0%
	GCTCAGATGCAGTGAAGTTG+TGG	-	chr2.4:72707816-72707835	MS.gene01687:CDS	50.0%
	TCTGAGCCACTCAAGTCTCC+GGG	+	chr2.4:72707803-72707822	None:intergenic	55.0%
	TGTCAACTGATCCACTCCGG+TGG	-	chr2.4:72707749-72707768	MS.gene01687:CDS	55.0%
	ATCCACTCCGGTGGCCGTTA+CGG	-	chr2.4:72707758-72707777	MS.gene01687:CDS	60.0%
	ATTCTCCCCGTAACGGCCAC+CGG	+	chr2.4:72707768-72707787	None:intergenic	60.0%
	CCGCGAGATTCTCCCCGTAA+CGG	+	chr2.4:72707775-72707794	None:intergenic	60.0%
	CCGTTACGGGGAGAATCTCG+CGG	-	chr2.4:72707772-72707791	MS.gene01687:CDS	60.0%
	CGTTACGGGGAGAATCTCGC+GGG	-	chr2.4:72707773-72707792	MS.gene01687:CDS	60.0%
	CTGAGCCACTCAAGTCTCCG+GGG	+	chr2.4:72707802-72707821	None:intergenic	60.0%
	GAGAATCTCGCGGGTAGCCC+CGG	-	chr2.4:72707782-72707801	MS.gene01687:CDS	65.0%
	GGTAGCCCCGGAGACTTGAG+TGG	-	chr2.4:72707794-72707813	MS.gene01687:CDS	65.0%
	TCCACTCCGGTGGCCGTTAC+GGG	-	chr2.4:72707759-72707778	MS.gene01687:CDS	65.0%
	CCACTCCGGTGGCCGTTACG+GGG	-	chr2.4:72707760-72707779	MS.gene01687:CDS	70.0%
	CCCCGTAACGGCCACCGGAG+TGG	+	chr2.4:72707763-72707782	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	72707554	72708033	72707554	ID=MS.gene01687
chr2.4	mRNA	72707554	72708033	72707554	ID=MS.gene01687.t1;Parent=MS.gene01687
chr2.4	exon	72707554	72708033	72707554	ID=MS.gene01687.t1.exon1;Parent=MS.gene01687.t1
chr2.4	CDS	72707554	72708033	72707554	ID=cds.MS.gene01687.t1;Parent=MS.gene01687.t1

Gene Sequence

>MS.gene01687

ATGAGTTTATTTTCTCTATTGTGTTTCTTGGGCTTATTACTCATTATTGGTCATGTTGCACATGCCCAAGACTCACAAGCAGACTATGTGAACGCTCACAACGAAGCAAGATCTGAGGTTGGTGTTCAAAATATTGTTTGGGACAATACTGTTGCTAGTTTTGCACAAGACTATGCTAATCAACACAAGGGTGATTGTCAACTGATCCACTCCGGTGGCCGTTACGGGGAGAATCTCGCGGGTAGCCCCGGAGACTTGAGTGGCTCAGATGCAGTGAAGTTGTGGGTGAATGAGAAAGTTGACTATGATTATAATAGTAACACATGTGCTTCTGGTAAAGTGTGTGGCCATTATACTCAGGTTGTATGGAGAAACTCACAACGTCTTGGATGTGCCAAAGTGAGATGTGATAATGGAGGCACATTCATTACTTGCAATTACGACCCACCAGGCAACTATAATAACGAAAAGCCATACTAA

Protein sequence

>MS.gene01687.t1

MSLFSLLCFLGLLLIIGHVAHAQDSQADYVNAHNEARSEVGVQNIVWDNTVASFAQDYANQHKGDCQLIHSGGRYGENLAGSPGDLSGSDAVKLWVNEKVDYDYNSNTCASGKVCGHYTQVVWRNSQRLGCAKVRCDNGGTFITCNYDPPGNYNNEKPY