AlfalfaGEDB | MS.gene01685

M. sativa cultivar XinJiangDaYe / MS.gene01685

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01685.t1	RHN71757.1	86.5	156	21	0	1	156	280	435	8.60E-80	306.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01685.t1	Q9ZNS4	68.1	141	40	3	16	156	26	161	3.8e-52	205.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01685.t1	G7IFD1	86.5	156	21	0	1	156	7	162	6.2e-80	306.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene01685	MS.gene002293	PPI
MS.gene00249	MS.gene01685	PPI
MS.gene028545	MS.gene01685	PPI
MS.gene02254	MS.gene01685	PPI
MS.gene018353	MS.gene01685	PPI
MS.gene01685	MS.gene57900	PPI
MS.gene002299	MS.gene01685	PPI
MS.gene02255	MS.gene01685	PPI
MS.gene01685	MS.gene01270	PPI
MS.gene01685	MS.gene002296	PPI
MS.gene01685	MS.gene002297	PPI
MS.gene01272	MS.gene01685	PPI
MS.gene01685	MS.gene01277	PPI
MS.gene02256	MS.gene01685	PPI
MS.gene01688	MS.gene01685	PPI
MS.gene01356	MS.gene01685	PPI
MS.gene01273	MS.gene01685	PPI
MS.gene01685	MS.gene002294	PPI
MS.gene01685	MS.gene01274	PPI
MS.gene01686	MS.gene01685	PPI
MS.gene012420	MS.gene01685	PPI
MS.gene01685	MS.gene002292	PPI
MS.gene002295	MS.gene01685	PPI
MS.gene02252	MS.gene01685	PPI
MS.gene035685	MS.gene01685	PPI
MS.gene02249	MS.gene01685	PPI
MS.gene01275	MS.gene01685	PPI
MS.gene01685	MS.gene02258	PPI
MS.gene01687	MS.gene01685	PPI
MS.gene01685	MS.gene038936	PPI
MS.gene01277	MS.gene01685	PPI
MS.gene035687	MS.gene01685	PPI
MS.gene01690	MS.gene01685	PPI
MS.gene01684	MS.gene01685	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01685.t1	MTR_2g010600	86.538	156	21	0	1	156	7	162	1.83e-104	295
MS.gene01685.t1	MTR_4g050762	86.486	148	20	0	9	156	15	162	2.49e-98	281
MS.gene01685.t1	MTR_2g012370	86.486	148	20	0	9	156	15	162	2.49e-98	281
MS.gene01685.t1	MTR_2g010590	83.333	156	23	2	1	156	7	159	1.51e-93	268
MS.gene01685.t1	MTR_2g010630	72.078	154	36	4	8	156	14	165	2.10e-77	227
MS.gene01685.t1	MTR_2g010650	68.519	162	41	6	1	156	8	165	6.85e-76	223
MS.gene01685.t1	MTR_2g010610	70.270	148	20	3	9	156	15	138	8.25e-71	209
MS.gene01685.t1	MTR_2g435490	56.954	151	63	1	6	156	16	164	4.96e-66	199
MS.gene01685.t1	MTR_2g010700	60.366	164	55	5	1	156	7	168	1.72e-65	197
MS.gene01685.t1	MTR_2g010640	59.375	160	58	5	1	156	7	163	5.31e-62	188
MS.gene01685.t1	MTR_2g010670	59.119	159	57	5	3	156	9	164	3.83e-57	176
MS.gene01685.t1	MTR_2g010690	55.346	159	63	5	3	156	9	164	2.89e-54	169
MS.gene01685.t1	MTR_2g010580	47.799	159	74	4	3	156	10	164	2.91e-52	164
MS.gene01685.t1	MTR_4g092540	48.408	157	76	4	1	156	7	159	1.97e-49	156
MS.gene01685.t1	MTR_4g128750	51.111	135	63	2	23	156	41	173	1.11e-44	145
MS.gene01685.t1	MTR_4g128770	45.652	138	71	3	22	156	70	206	5.07e-41	136
MS.gene01685.t1	MTR_1g067290	37.956	137	80	4	22	155	73	207	2.38e-31	112
MS.gene01685.t1	MTR_8g078770	38.406	138	81	4	22	156	36	172	6.11e-27	99.8
MS.gene01685.t1	MTR_4g092515	52.941	102	41	3	1	97	7	106	1.36e-25	95.1
MS.gene01685.t1	MTR_5g018770	34.043	141	85	5	22	156	47	185	1.62e-24	94.0
MS.gene01685.t1	MTR_5g018755	30.851	94	60	3	68	156	10	103	5.10e-13	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01685.t1	AT4G33720	60.256	156	59	2	1	156	11	163	1.32e-66	200
MS.gene01685.t1	AT2G14580	68.085	141	40	3	16	156	26	161	5.21e-64	193
MS.gene01685.t1	AT5G26130	58.974	156	63	1	1	156	12	166	6.12e-64	193
MS.gene01685.t1	AT2G14610	62.937	143	48	3	14	156	24	161	1.66e-60	184
MS.gene01685.t1	AT4G33710	61.702	141	53	1	16	156	27	166	1.91e-59	182
MS.gene01685.t1	AT3G19690	53.797	158	66	4	1	156	9	161	5.54e-57	176
MS.gene01685.t1	AT4G25790	53.676	136	60	2	22	156	77	210	1.91e-50	160
MS.gene01685.t1	AT1G50060	55.629	151	61	4	7	156	16	161	1.60e-49	157
MS.gene01685.t1	AT2G19990	50.365	137	66	1	20	156	42	176	3.11e-49	156
MS.gene01685.t1	AT5G57625	52.593	135	61	2	23	156	75	207	8.60e-49	156
MS.gene01685.t1	AT4G25780	50.725	138	64	3	22	156	54	190	4.19e-45	146
MS.gene01685.t1	AT3G09590	48.905	137	66	2	22	156	52	186	4.24e-45	146
MS.gene01685.t1	AT4G30320	45.695	151	78	3	7	156	14	161	1.34e-44	144
MS.gene01685.t1	AT1G50050	56.954	151	60	4	7	156	16	162	2.72e-44	143
MS.gene01685.t1	AT4G33730	51.449	138	62	4	20	156	39	172	1.43e-43	142
MS.gene01685.t1	AT5G02730	45.985	137	70	2	22	156	59	193	2.21e-40	135
MS.gene01685.t1	AT1G50050	53.548	155	63	4	7	154	8	160	1.62e-39	133
MS.gene01685.t1	AT4G31470	46.324	136	69	3	22	156	53	185	1.23e-38	130
MS.gene01685.t1	AT1G01310	44.853	136	71	3	22	156	87	219	4.58e-38	130
MS.gene01685.t1	AT5G66590	45.714	140	67	3	23	156	49	185	1.26e-37	127
MS.gene01685.t1	AT4G07820	44.366	142	74	4	15	156	24	160	7.35e-37	124
MS.gene01685.t1	AT2G19970	39.706	136	69	6	20	146	35	166	6.54e-25	94.7
MS.gene01685.t1	AT2G19980	37.324	142	73	6	20	156	35	165	1.13e-22	88.6

Find 41 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATAGTCTGTTGGTGAGTTT+TGG	0.180317	2.4:+72721884	None:intergenic
CCGGTGGTGGTGGCCGTTAC+GGG	0.193193	2.4:-72721738	MS.gene01685:CDS
TTAGGCTTATTTCTCATTAT+TGG	0.210544	2.4:-72721922	MS.gene01685:CDS
TCCGGTGGTGGTGGCCGTTA+CGG	0.303334	2.4:-72721739	MS.gene01685:CDS
TATTAAACATGCACTTAGTA+TGG	0.322729	2.4:+72721469	None:intergenic
TTGGTGTTGCAAATGTTGTT+TGG	0.348141	2.4:-72721831	MS.gene01685:CDS
GCCATTATACTCAGGTGGTT+TGG	0.358578	2.4:-72721597	MS.gene01685:CDS
GTTACTATTATAGTTATAAT+CGG	0.390895	2.4:+72721645	None:intergenic
TCCAAACCACCTGAGTATAA+TGG	0.398812	2.4:+72721596	None:intergenic
TGGTGTTGCAAATGTTGTTT+GGG	0.411343	2.4:-72721830	MS.gene01685:CDS
CTGACGTCTTGCTTCGTTGT+GGG	0.413555	2.4:+72721855	None:intergenic
CCCGTAACGGCCACCACCAC+CGG	0.429882	2.4:+72721738	None:intergenic
TTGATCCACTCCGGTGGTGG+TGG	0.451842	2.4:-72721748	MS.gene01685:CDS
TGGAGAAACACAAAGCGTAT+TGG	0.455942	2.4:-72721577	MS.gene01685:CDS
GCACAGATGCAGTGAGGTTG+TGG	0.462999	2.4:-72721681	MS.gene01685:CDS
ATTATTATCACATTTCACTT+TGG	0.463790	2.4:+72721549	None:intergenic
GTGAAATGTGATAATAATAG+AGG	0.492513	2.4:-72721544	MS.gene01685:CDS
GAGAATCTAGCGTGGAGCTC+CGG	0.500119	2.4:-72721715	MS.gene01685:CDS
ATCTGTGCCACTCATGTCGC+CGG	0.501762	2.4:+72721696	None:intergenic
CCTGACGTCTTGCTTCGTTG+TGG	0.534137	2.4:+72721854	None:intergenic
CACAGATGCAGTGAGGTTGT+GGG	0.544377	2.4:-72721680	MS.gene01685:CDS
CAATTGATCCACTCCGGTGG+TGG	0.548354	2.4:-72721751	MS.gene01685:CDS
CGGTGGTGGTGGCCGTTACG+GGG	0.566376	2.4:-72721737	MS.gene01685:CDS
CCACAACGAAGCAAGACGTC+AGG	0.568340	2.4:-72721854	MS.gene01685:CDS
TGGGCGTTCACATAGTCTGT+TGG	0.581596	2.4:+72721874	None:intergenic
AAACTATGCTAATCAACGCA+AGG	0.596763	2.4:-72721782	MS.gene01685:CDS
TGGAGCTCCGGCGACATGAG+TGG	0.603470	2.4:-72721703	MS.gene01685:CDS
GATCCACCAGGCAACTATGT+TGG	0.607501	2.4:-72721499	MS.gene01685:CDS
AACGGCCACCACCACCGGAG+TGG	0.614459	2.4:+72721743	None:intergenic
TGACCAACATAGTTGCCTGG+TGG	0.614666	2.4:+72721496	None:intergenic
AACTATGCTAATCAACGCAA+GGG	0.616329	2.4:-72721781	MS.gene01685:CDS
GTGTGGCCATTATACTCAGG+TGG	0.627875	2.4:-72721602	MS.gene01685:CDS
GATTGTCAATTGATCCACTC+CGG	0.634101	2.4:-72721757	MS.gene01685:CDS
AGTGTGTGGCCATTATACTC+AGG	0.651003	2.4:-72721605	MS.gene01685:CDS
AACGAAGCAAGACGTCAGGT+TGG	0.655351	2.4:-72721850	MS.gene01685:CDS
ACGCTAGATTCTCCCCGTAA+CGG	0.657514	2.4:+72721725	None:intergenic
ACTTGCAATTACGATCCACC+AGG	0.660677	2.4:-72721511	MS.gene01685:CDS
TGTCAATTGATCCACTCCGG+TGG	0.707723	2.4:-72721754	MS.gene01685:CDS
GTTACGGGGAGAATCTAGCG+TGG	0.716019	2.4:-72721723	MS.gene01685:CDS
TGTGCTGCTAATAAAGTGTG+TGG	0.730769	2.4:-72721619	MS.gene01685:CDS
TGAGTGGCACAGATGCAGTG+AGG	0.786885	2.4:-72721687	MS.gene01685:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTACTATTATAGTTATAAT+CGG	+	chr2.4:72721772-72721791	None:intergenic	15.0%
!!	ATTATTATCACATTTCACTT+TGG	+	chr2.4:72721868-72721887	None:intergenic	20.0%
!	GTGAAATGTGATAATAATAG+AGG	-	chr2.4:72721870-72721889	MS.gene01685:CDS	25.0%
!!	TTAGGCTTATTTCTCATTAT+TGG	-	chr2.4:72721492-72721511	MS.gene01685:CDS	25.0%
	AAACTATGCTAATCAACGCA+AGG	-	chr2.4:72721632-72721651	MS.gene01685:CDS	35.0%
	AACTATGCTAATCAACGCAA+GGG	-	chr2.4:72721633-72721652	MS.gene01685:CDS	35.0%
!!	ATAGTCTGTTGGTGAGTTTT+GGG	+	chr2.4:72721532-72721551	None:intergenic	35.0%
!!	TGGTGTTGCAAATGTTGTTT+GGG	-	chr2.4:72721584-72721603	MS.gene01685:CDS	35.0%
!!	TTGGTGTTGCAAATGTTGTT+TGG	-	chr2.4:72721583-72721602	MS.gene01685:CDS	35.0%
	GATTGTCAATTGATCCACTC+CGG	-	chr2.4:72721657-72721676	MS.gene01685:CDS	40.0%
	TCCAAACCACCTGAGTATAA+TGG	+	chr2.4:72721821-72721840	None:intergenic	40.0%
	TGGAGAAACACAAAGCGTAT+TGG	-	chr2.4:72721837-72721856	MS.gene01685:CDS	40.0%
!	TGTGCTGCTAATAAAGTGTG+TGG	-	chr2.4:72721795-72721814	MS.gene01685:CDS	40.0%
!	TTTTGACCAACATAGTTGCC+TGG	+	chr2.4:72721924-72721943	None:intergenic	40.0%
!!	CATAGTCTGTTGGTGAGTTT+TGG	+	chr2.4:72721533-72721552	None:intergenic	40.0%
	ACTTGCAATTACGATCCACC+AGG	-	chr2.4:72721903-72721922	MS.gene01685:CDS	45.0%
	AGTGTGTGGCCATTATACTC+AGG	-	chr2.4:72721809-72721828	MS.gene01685:CDS	45.0%
	GCCATTATACTCAGGTGGTT+TGG	-	chr2.4:72721817-72721836	MS.gene01685:CDS	45.0%
	AACGAAGCAAGACGTCAGGT+TGG	-	chr2.4:72721564-72721583	MS.gene01685:CDS	50.0%
	ACGCTAGATTCTCCCCGTAA+CGG	+	chr2.4:72721692-72721711	None:intergenic	50.0%
	CACAGATGCAGTGAGGTTGT+GGG	-	chr2.4:72721734-72721753	MS.gene01685:CDS	50.0%
	CTGACGTCTTGCTTCGTTGT+GGG	+	chr2.4:72721562-72721581	None:intergenic	50.0%
	GATCCACCAGGCAACTATGT+TGG	-	chr2.4:72721915-72721934	MS.gene01685:CDS	50.0%
	GTGTGGCCATTATACTCAGG+TGG	-	chr2.4:72721812-72721831	MS.gene01685:CDS	50.0%
	TGACCAACATAGTTGCCTGG+TGG	+	chr2.4:72721921-72721940	None:intergenic	50.0%
	TGGGCGTTCACATAGTCTGT+TGG	+	chr2.4:72721543-72721562	None:intergenic	50.0%
	TGTCAATTGATCCACTCCGG+TGG	-	chr2.4:72721660-72721679	MS.gene01685:CDS	50.0%
	CAATTGATCCACTCCGGTGG+TGG	-	chr2.4:72721663-72721682	MS.gene01685:CDS	55.0%
	CCACAACGAAGCAAGACGTC+AGG	-	chr2.4:72721560-72721579	MS.gene01685:CDS	55.0%
	CCTGACGTCTTGCTTCGTTG+TGG	+	chr2.4:72721563-72721582	None:intergenic	55.0%
	GAGAATCTAGCGTGGAGCTC+CGG	-	chr2.4:72721699-72721718	MS.gene01685:CDS	55.0%
	GCACAGATGCAGTGAGGTTG+TGG	-	chr2.4:72721733-72721752	MS.gene01685:CDS	55.0%
	GTTACGGGGAGAATCTAGCG+TGG	-	chr2.4:72721691-72721710	MS.gene01685:CDS	55.0%
	TGAGTGGCACAGATGCAGTG+AGG	-	chr2.4:72721727-72721746	MS.gene01685:CDS	55.0%
!	ATCTGTGCCACTCATGTCGC+CGG	+	chr2.4:72721721-72721740	None:intergenic	55.0%
!	TTGATCCACTCCGGTGGTGG+TGG	-	chr2.4:72721666-72721685	MS.gene01685:CDS	60.0%
	TGGAGCTCCGGCGACATGAG+TGG	-	chr2.4:72721711-72721730	MS.gene01685:CDS	65.0%
!!	TCCGGTGGTGGTGGCCGTTA+CGG	-	chr2.4:72721675-72721694	MS.gene01685:CDS	65.0%
	AACGGCCACCACCACCGGAG+TGG	+	chr2.4:72721674-72721693	None:intergenic	70.0%
	CCCGTAACGGCCACCACCAC+CGG	+	chr2.4:72721679-72721698	None:intergenic	70.0%
!!	CCGGTGGTGGTGGCCGTTAC+GGG	-	chr2.4:72721676-72721695	MS.gene01685:CDS	70.0%
!!	CGGTGGTGGTGGCCGTTACG+GGG	-	chr2.4:72721677-72721696	MS.gene01685:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	72721483	72721953	72721483	ID=MS.gene01685
chr2.4	mRNA	72721483	72721953	72721483	ID=MS.gene01685.t1;Parent=MS.gene01685
chr2.4	exon	72721483	72721953	72721483	ID=MS.gene01685.t1.exon1;Parent=MS.gene01685.t1
chr2.4	CDS	72721483	72721953	72721483	ID=cds.MS.gene01685.t1;Parent=MS.gene01685.t1

Gene Sequence

>MS.gene01685

ATGTGTGTCTTAGGCTTATTTCTCATTATTGGTCACGTTGCACATGCCCAAAACTCACCAACAGACTATGTGAACGCCCACAACGAAGCAAGACGTCAGGTTGGTGTTGCAAATGTTGTTTGGGACAATACTGTTGCTGCTTTTGCACAAAACTATGCTAATCAACGCAAGGGTGATTGTCAATTGATCCACTCCGGTGGTGGTGGCCGTTACGGGGAGAATCTAGCGTGGAGCTCCGGCGACATGAGTGGCACAGATGCAGTGAGGTTGTGGGTGAATGAGAGAGCCGATTATAACTATAATAGTAACACATGTGCTGCTAATAAAGTGTGTGGCCATTATACTCAGGTGGTTTGGAGAAACACAAAGCGTATTGGATGTGCCAAAGTGAAATGTGATAATAATAGAGGAACATTCATTACTTGCAATTACGATCCACCAGGCAACTATGTTGGTCAAAAGCCATACTAA

Protein sequence

>MS.gene01685.t1

MCVLGLFLIIGHVAHAQNSPTDYVNAHNEARRQVGVANVVWDNTVAAFAQNYANQRKGDCQLIHSGGGGRYGENLAWSSGDMSGTDAVRLWVNERADYNYNSNTCAANKVCGHYTQVVWRNTKRIGCAKVKCDNNRGTFITCNYDPPGNYVGQKPY