AlfalfaGEDB | MS.gene01690

M. sativa cultivar XinJiangDaYe / MS.gene01690

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01690.t1	XP_003593363.1	96.6	146	5	0	3	148	20	165	1.30E-80	308.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01690.t1	Q9ZNS4	63.4	142	45	4	8	148	26	161	5.4e-48	191.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01690.t1	G7IFD6	96.6	146	5	0	3	148	20	165	9.1e-81	308.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene02254	MS.gene01690	PPI
MS.gene01273	MS.gene01690	PPI
MS.gene018353	MS.gene01690	PPI
MS.gene002299	MS.gene01690	PPI
MS.gene01690	MS.gene038936	PPI
MS.gene01690	MS.gene02252	PPI
MS.gene01272	MS.gene01690	PPI
MS.gene01690	MS.gene002293	PPI
MS.gene01690	MS.gene01270	PPI
MS.gene01688	MS.gene01690	PPI
MS.gene01356	MS.gene01690	PPI
MS.gene02249	MS.gene01690	PPI
MS.gene01275	MS.gene01690	PPI
MS.gene01690	MS.gene57900	PPI
MS.gene012420	MS.gene01690	PPI
MS.gene01687	MS.gene01690	PPI
MS.gene01690	MS.gene01356	PPI
MS.gene01690	MS.gene002296	PPI
MS.gene01690	MS.gene002297	PPI
MS.gene01690	MS.gene01277	PPI
MS.gene01690	MS.gene02256	PPI
MS.gene035685	MS.gene01690	PPI
MS.gene01690	MS.gene01686	PPI
MS.gene01690	MS.gene002295	PPI
MS.gene01690	MS.gene002294	PPI
MS.gene01690	MS.gene01274	PPI
MS.gene028545	MS.gene01690	PPI
MS.gene01690	MS.gene02258	PPI
MS.gene01690	MS.gene002292	PPI
MS.gene01684	MS.gene01690	PPI
MS.gene02255	MS.gene01690	PPI
MS.gene01690	MS.gene01685	PPI
MS.gene01690	MS.gene035687	PPI
MS.gene00249	MS.gene01690	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01690.t1	MTR_2g010650	96.575	146	5	0	3	148	20	165	7.56e-106	299
MS.gene01690.t1	MTR_2g010630	88.667	150	15	2	1	148	16	165	1.88e-95	273
MS.gene01690.t1	MTR_2g010700	70.779	154	38	4	1	148	16	168	2.07e-74	219
MS.gene01690.t1	MTR_2g010600	72.483	149	33	5	4	148	18	162	9.57e-73	215
MS.gene01690.t1	MTR_2g010640	69.128	149	44	2	1	148	16	163	1.71e-72	214
MS.gene01690.t1	MTR_2g010670	69.128	149	45	1	1	148	16	164	4.95e-72	213
MS.gene01690.t1	MTR_4g050762	71.141	149	35	5	4	148	18	162	3.99e-70	209
MS.gene01690.t1	MTR_2g012370	71.141	149	35	5	4	148	18	162	3.99e-70	209
MS.gene01690.t1	MTR_2g010590	67.347	147	41	4	4	148	18	159	4.42e-70	208
MS.gene01690.t1	MTR_2g010690	65.772	149	50	1	1	148	16	164	1.49e-69	207
MS.gene01690.t1	MTR_2g010610	61.905	147	28	5	4	148	18	138	3.15e-56	172
MS.gene01690.t1	MTR_2g435490	55.034	149	59	5	3	148	21	164	1.57e-53	167
MS.gene01690.t1	MTR_2g010580	53.103	145	62	3	5	148	25	164	5.18e-53	165
MS.gene01690.t1	MTR_4g092540	49.333	150	69	4	1	148	15	159	4.45e-48	152
MS.gene01690.t1	MTR_4g128750	55.072	138	53	6	15	148	41	173	6.37e-40	132
MS.gene01690.t1	MTR_4g128770	48.227	141	63	6	14	148	70	206	1.06e-39	133
MS.gene01690.t1	MTR_1g067290	39.286	140	74	6	14	147	73	207	4.62e-27	100
MS.gene01690.t1	MTR_8g078770	34.752	141	82	5	14	148	36	172	3.88e-22	87.0
MS.gene01690.t1	MTR_5g018770	32.168	143	85	5	14	148	47	185	2.43e-20	82.8
MS.gene01690.t1	MTR_4g092515	53.846	78	29	4	6	81	24	96	4.72e-18	75.5
MS.gene01690.t1	MTR_5g018755	34.653	101	59	5	55	148	3	103	8.02e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01690.t1	AT2G14580	63.380	142	45	4	8	148	26	161	8.12e-59	180
MS.gene01690.t1	AT4G33720	60.839	143	49	4	8	148	26	163	5.03e-58	178
MS.gene01690.t1	AT3G19690	59.310	145	50	4	8	148	22	161	1.95e-56	174
MS.gene01690.t1	AT2G14610	58.333	144	53	4	6	148	24	161	4.62e-56	173
MS.gene01690.t1	AT5G26130	57.639	144	54	4	8	148	27	166	5.53e-53	165
MS.gene01690.t1	AT4G33710	56.944	144	55	4	8	148	27	166	9.87e-53	164
MS.gene01690.t1	AT1G50060	57.534	146	54	5	6	148	21	161	1.01e-50	159
MS.gene01690.t1	AT4G33730	51.351	148	65	3	3	148	30	172	1.75e-47	151
MS.gene01690.t1	AT1G50050	52.414	145	62	4	7	148	22	162	2.17e-42	138
MS.gene01690.t1	AT4G25790	49.640	139	61	6	14	148	77	210	3.65e-42	139
MS.gene01690.t1	AT5G57625	50.000	138	60	6	15	148	75	207	4.55e-41	136
MS.gene01690.t1	AT2G19990	48.571	140	64	5	12	148	42	176	8.34e-41	135
MS.gene01690.t1	AT4G25780	48.936	141	62	5	14	148	54	190	4.46e-39	130
MS.gene01690.t1	AT4G30320	46.763	139	70	4	13	148	24	161	2.32e-38	128
MS.gene01690.t1	AT1G50050	51.825	137	59	4	7	140	22	154	9.39e-38	128
MS.gene01690.t1	AT3G09590	46.429	140	65	6	14	148	52	186	5.64e-37	125
MS.gene01690.t1	AT4G07820	44.056	143	73	4	7	148	24	160	8.83e-37	124
MS.gene01690.t1	AT4G31470	42.754	138	71	5	14	148	53	185	8.27e-36	122
MS.gene01690.t1	AT5G02730	46.809	141	65	6	13	148	58	193	6.90e-35	120
MS.gene01690.t1	AT1G01310	44.928	138	68	5	14	148	87	219	2.87e-32	115
MS.gene01690.t1	AT5G66590	37.589	141	77	5	15	148	49	185	3.45e-27	100
MS.gene01690.t1	AT2G19980	39.583	144	67	6	12	148	35	165	3.84e-23	89.4
MS.gene01690.t1	AT2G19970	38.255	149	74	7	12	148	35	177	5.31e-22	87.0

Find 38 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTTGTCCCAAACAATATT+TGG	0.198843	2.4:+72697739	None:intergenic
TTAAAATTCCAAATATTGTT+TGG	0.293275	2.4:-72697747	MS.gene01690:CDS
GATTGTCAATTGGTCCATTC+TGG	0.325775	2.4:-72697676	MS.gene01690:CDS
TCCATTATACTCAGGTGGTT+TGG	0.346423	2.4:-72697522	MS.gene01690:CDS
TAAAATTCCAAATATTGTTT+GGG	0.350860	2.4:-72697746	MS.gene01690:CDS
CTGCCGATCTTGCTTGGTTG+TGG	0.358764	2.4:+72697782	None:intergenic
CAAATATGAATCACTTAGTA+TGG	0.381615	2.4:+72697397	None:intergenic
TTACTACTATAATCGTAGTT+TGG	0.383101	2.4:+72697568	None:intergenic
TCCAAACCACCTGAGTATAA+TGG	0.384825	2.4:+72697521	None:intergenic
CATTCTGGTGGTGACCGTTA+TGG	0.426730	2.4:-72697661	MS.gene01690:CDS
TGCCGATCTTGCTTGGTTGT+GGG	0.426868	2.4:+72697783	None:intergenic
GCCAAAGTGAAATGTGATAA+TGG	0.427399	2.4:-72697475	MS.gene01690:CDS
TATCAACTGCCGATCTTGCT+TGG	0.450687	2.4:+72697776	None:intergenic
TGGAGAAACACACAACGTAT+TGG	0.459583	2.4:-72697502	MS.gene01690:CDS
TCCATTATCACATTTCACTT+TGG	0.469667	2.4:+72697474	None:intergenic
ACTCATGTCGCCGCTGCTCT+CGG	0.470748	2.4:+72697627	None:intergenic
CTTTCGCCAATATAGTTACC+AGG	0.476669	2.4:+72697421	None:intergenic
TCAACGCAAGGATTGTCAAT+TGG	0.501362	2.4:-72697686	MS.gene01690:CDS
GCATAGAAGCAGTGAAGTTG+TGG	0.504180	2.4:-72697603	MS.gene01690:CDS
TGCCCACAACCAAGCAAGAT+CGG	0.521741	2.4:-72697785	MS.gene01690:CDS
TGGGCATTCACGTAGTCTGC+TGG	0.533658	2.4:+72697802	None:intergenic
GAGAATATCGCCGAGAGCAG+CGG	0.550660	2.4:-72697637	MS.gene01690:CDS
CATAACGGTCACCACCAGAA+TGG	0.563682	2.4:+72697662	None:intergenic
TATAGTAGTAACTCATGTGT+TGG	0.568160	2.4:-72697556	MS.gene01690:CDS
CGGCGATATTCTCTCCATAA+CGG	0.600779	2.4:+72697647	None:intergenic
CATAGAAGCAGTGAAGTTGT+GGG	0.603029	2.4:-72697602	MS.gene01690:CDS
TGTCAATTGGTCCATTCTGG+TGG	0.605326	2.4:-72697673	MS.gene01690:CDS
GAGAGCAGCGGCGACATGAG+TGG	0.607916	2.4:-72697625	MS.gene01690:CDS
AAATTATGCTAATCAACGCA+AGG	0.622269	2.4:-72697698	MS.gene01690:CDS
ATGCCTCCATTATACTCAGG+TGG	0.625424	2.4:-72697527	MS.gene01690:CDS
TTTCGCCAATATAGTTACCA+GGG	0.639472	2.4:+72697422	None:intergenic
TGAATGCCTCCATTATACTC+AGG	0.648037	2.4:-72697530	MS.gene01690:CDS
AGTAGTAACTCATGTGTTGG+TGG	0.657995	2.4:-72697553	MS.gene01690:CDS
TTCGCCAATATAGTTACCAG+GGG	0.660707	2.4:+72697423	None:intergenic
AAACCACCTGAGTATAATGG+AGG	0.693168	2.4:+72697524	None:intergenic
TCGCCAATATAGTTACCAGG+GGG	0.693793	2.4:+72697424	None:intergenic
CGCCAATATAGTTACCAGGG+GGG	0.703780	2.4:+72697425	None:intergenic
AAAGTGAAATGTGATAATGG+AGG	0.772799	2.4:-72697472	MS.gene01690:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATTCCAAATATTGTTT+GGG	-	chr2.4:72697500-72697519	MS.gene01690:CDS	15.0%
!!	TTAAAATTCCAAATATTGTT+TGG	-	chr2.4:72697499-72697518	MS.gene01690:CDS	15.0%
!	TTACTACTATAATCGTAGTT+TGG	+	chr2.4:72697681-72697700	None:intergenic	25.0%
	AAATTATGCTAATCAACGCA+AGG	-	chr2.4:72697548-72697567	MS.gene01690:CDS	30.0%
	TATAGTAGTAACTCATGTGT+TGG	-	chr2.4:72697690-72697709	MS.gene01690:CDS	30.0%
	TCCATTATCACATTTCACTT+TGG	+	chr2.4:72697775-72697794	None:intergenic	30.0%
	TTGTTGTCCCAAACAATATT+TGG	+	chr2.4:72697510-72697529	None:intergenic	30.0%
!!	AAAGTGAAATGTGATAATGG+AGG	-	chr2.4:72697774-72697793	MS.gene01690:CDS	30.0%
	GCCAAAGTGAAATGTGATAA+TGG	-	chr2.4:72697771-72697790	MS.gene01690:CDS	35.0%
	TTTCGCCAATATAGTTACCA+GGG	+	chr2.4:72697827-72697846	None:intergenic	35.0%
	AAACCACCTGAGTATAATGG+AGG	+	chr2.4:72697725-72697744	None:intergenic	40.0%
	AGTAGTAACTCATGTGTTGG+TGG	-	chr2.4:72697693-72697712	MS.gene01690:CDS	40.0%
	CATAGAAGCAGTGAAGTTGT+GGG	-	chr2.4:72697644-72697663	MS.gene01690:CDS	40.0%
	CTTTCGCCAATATAGTTACC+AGG	+	chr2.4:72697828-72697847	None:intergenic	40.0%
	GATTGTCAATTGGTCCATTC+TGG	-	chr2.4:72697570-72697589	MS.gene01690:CDS	40.0%
	TCAACGCAAGGATTGTCAAT+TGG	-	chr2.4:72697560-72697579	MS.gene01690:CDS	40.0%
	TCCAAACCACCTGAGTATAA+TGG	+	chr2.4:72697728-72697747	None:intergenic	40.0%
	TCCATTATACTCAGGTGGTT+TGG	-	chr2.4:72697724-72697743	MS.gene01690:CDS	40.0%
	TGAATGCCTCCATTATACTC+AGG	-	chr2.4:72697716-72697735	MS.gene01690:CDS	40.0%
	TGGAGAAACACACAACGTAT+TGG	-	chr2.4:72697744-72697763	MS.gene01690:CDS	40.0%
	TTCGCCAATATAGTTACCAG+GGG	+	chr2.4:72697826-72697845	None:intergenic	40.0%
	ATGCCTCCATTATACTCAGG+TGG	-	chr2.4:72697719-72697738	MS.gene01690:CDS	45.0%
	CGGCGATATTCTCTCCATAA+CGG	+	chr2.4:72697602-72697621	None:intergenic	45.0%
	GCATAGAAGCAGTGAAGTTG+TGG	-	chr2.4:72697643-72697662	MS.gene01690:CDS	45.0%
	TATCAACTGCCGATCTTGCT+TGG	+	chr2.4:72697473-72697492	None:intergenic	45.0%
	TCGCCAATATAGTTACCAGG+GGG	+	chr2.4:72697825-72697844	None:intergenic	45.0%
	TGTCAATTGGTCCATTCTGG+TGG	-	chr2.4:72697573-72697592	MS.gene01690:CDS	45.0%
	CATAACGGTCACCACCAGAA+TGG	+	chr2.4:72697587-72697606	None:intergenic	50.0%
	CGCCAATATAGTTACCAGGG+GGG	+	chr2.4:72697824-72697843	None:intergenic	50.0%
	GACCCCCCTGGTAACTATAT+TGG	-	chr2.4:72697819-72697838	MS.gene01690:CDS	50.0%
	TGCCCACAACCAAGCAAGAT+CGG	-	chr2.4:72697461-72697480	MS.gene01690:CDS	50.0%
	TGCCGATCTTGCTTGGTTGT+GGG	+	chr2.4:72697466-72697485	None:intergenic	50.0%
!	CATTCTGGTGGTGACCGTTA+TGG	-	chr2.4:72697585-72697604	MS.gene01690:CDS	50.0%
	ACTTGCAATTACGACCCCCC+TGG	-	chr2.4:72697807-72697826	MS.gene01690:CDS	55.0%
	CTGCCGATCTTGCTTGGTTG+TGG	+	chr2.4:72697467-72697486	None:intergenic	55.0%
	GAGAATATCGCCGAGAGCAG+CGG	-	chr2.4:72697609-72697628	MS.gene01690:CDS	55.0%
	TGGGCATTCACGTAGTCTGC+TGG	+	chr2.4:72697447-72697466	None:intergenic	55.0%
	ACTCATGTCGCCGCTGCTCT+CGG	+	chr2.4:72697622-72697641	None:intergenic	60.0%
	GAGAGCAGCGGCGACATGAG+TGG	-	chr2.4:72697621-72697640	MS.gene01690:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	72697411	72697857	72697411	ID=MS.gene01690
chr2.4	mRNA	72697411	72697857	72697411	ID=MS.gene01690.t1;Parent=MS.gene01690
chr2.4	exon	72697411	72697857	72697411	ID=MS.gene01690.t1.exon1;Parent=MS.gene01690.t1
chr2.4	CDS	72697411	72697857	72697411	ID=cds.MS.gene01690.t1;Parent=MS.gene01690.t1

Gene Sequence

>MS.gene01690

ATGGATAGTCACGTTGCACATGCACAAGACTCACCAGCAGACTACGTGAATGCCCACAACCAAGCAAGATCGGCAGTTGATACAAACATTAAAATTCCAAATATTGTTTGGGACAACAACGTCGCTGCTTTTGCACAAAATTATGCTAATCAACGCAAGGATTGTCAATTGGTCCATTCTGGTGGTGACCGTTATGGAGAGAATATCGCCGAGAGCAGCGGCGACATGAGTGGCATAGAAGCAGTGAAGTTGTGGGTTGATGAAAAACCAAACTACGATTATAGTAGTAACTCATGTGTTGGTGGTGAATGCCTCCATTATACTCAGGTGGTTTGGAGAAACACACAACGTATTGGATGTGCCAAAGTGAAATGTGATAATGGAGGCACATTCATTACTTGCAATTACGACCCCCCTGGTAACTATATTGGCGAAAGACCATACTAA

Protein sequence

>MS.gene01690.t1

MDSHVAHAQDSPADYVNAHNQARSAVDTNIKIPNIVWDNNVAAFAQNYANQRKDCQLVHSGGDRYGENIAESSGDMSGIEAVKLWVDEKPNYDYSSNSCVGGECLHYTQVVWRNTQRIGCAKVKCDNGGTFITCNYDPPGNYIGERPY