AlfalfaGEDB | MS.gene02255

M. sativa cultivar XinJiangDaYe / MS.gene02255

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02255.t1	RHN71757.1	85.8	162	20	2	1	159	274	435	2.50E-74	288.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02255.t1	Q9ZNS4	65.0	157	50	2	3	159	10	161	1.4e-54	213.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02255.t1	G7IFD1	85.8	162	20	2	1	159	1	162	1.8e-74	288.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene00249	MS.gene02255	PPI
MS.gene02255	MS.gene002295	PPI
MS.gene02255	MS.gene002294	PPI
MS.gene02255	MS.gene01275	PPI
MS.gene02254	MS.gene02255	PPI
MS.gene02255	MS.gene02258	PPI
MS.gene01273	MS.gene02255	PPI
MS.gene018353	MS.gene02255	PPI
MS.gene02255	MS.gene002292	PPI
MS.gene002299	MS.gene02255	PPI
MS.gene01272	MS.gene02255	PPI
MS.gene02255	MS.gene01685	PPI
MS.gene02255	MS.gene035687	PPI
MS.gene01688	MS.gene02255	PPI
MS.gene02255	MS.gene038936	PPI
MS.gene012420	MS.gene02255	PPI
MS.gene02255	MS.gene02252	PPI
MS.gene01687	MS.gene02255	PPI
MS.gene02255	MS.gene002293	PPI
MS.gene02255	MS.gene01270	PPI
MS.gene02255	MS.gene02249	PPI
MS.gene035685	MS.gene02255	PPI
MS.gene02255	MS.gene57900	PPI
MS.gene028545	MS.gene02255	PPI
MS.gene02255	MS.gene01274	PPI
MS.gene02255	MS.gene01356	PPI
MS.gene02255	MS.gene002296	PPI
MS.gene02255	MS.gene002297	PPI
MS.gene02255	MS.gene01277	PPI
MS.gene01684	MS.gene02255	PPI
MS.gene02255	MS.gene02256	PPI
MS.gene02255	MS.gene01690	PPI
MS.gene02255	MS.gene01686	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02255.t1	MTR_2g010600	85.802	162	20	2	1	159	1	162	1.15e-98	281
MS.gene02255.t1	MTR_2g010590	78.616	159	34	0	1	159	1	159	2.19e-96	275
MS.gene02255.t1	MTR_4g050762	84.568	162	22	2	1	159	1	162	3.73e-90	260
MS.gene02255.t1	MTR_2g012370	84.568	162	22	2	1	159	1	162	3.73e-90	260
MS.gene02255.t1	MTR_2g010610	81.250	160	7	3	1	159	1	138	1.42e-78	229
MS.gene02255.t1	MTR_2g010650	68.263	167	43	6	1	159	1	165	1.11e-76	226
MS.gene02255.t1	MTR_2g010630	70.588	153	38	3	13	159	14	165	2.09e-75	222
MS.gene02255.t1	MTR_2g010700	60.355	169	56	5	1	159	1	168	4.59e-65	196
MS.gene02255.t1	MTR_2g010640	61.212	165	56	5	1	159	1	163	1.45e-63	192
MS.gene02255.t1	MTR_2g435490	54.487	156	69	2	6	159	9	164	1.37e-60	186
MS.gene02255.t1	MTR_2g010670	59.036	166	59	5	1	159	1	164	1.24e-58	180
MS.gene02255.t1	MTR_2g010690	56.627	166	63	5	1	159	1	164	4.11e-57	176
MS.gene02255.t1	MTR_2g010580	47.170	159	83	1	1	159	7	164	4.25e-54	168
MS.gene02255.t1	MTR_4g092540	49.375	160	70	3	1	159	10	159	2.24e-52	164
MS.gene02255.t1	MTR_4g128750	51.128	133	63	2	29	159	41	173	1.48e-42	139
MS.gene02255.t1	MTR_4g128770	44.526	137	71	2	28	159	70	206	3.24e-40	134
MS.gene02255.t1	MTR_1g067290	42.963	135	73	3	28	158	73	207	3.76e-34	119
MS.gene02255.t1	MTR_8g078770	35.329	167	94	5	7	159	6	172	7.59e-27	99.8
MS.gene02255.t1	MTR_4g092515	51.961	102	46	2	1	101	7	106	1.78e-26	97.4
MS.gene02255.t1	MTR_5g018770	34.270	178	95	6	3	159	9	185	2.18e-25	96.3
MS.gene02255.t1	MTR_5g018755	35.789	95	55	4	71	159	9	103	4.37e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02255.t1	AT2G14580	64.968	157	50	2	3	159	10	161	2.26e-70	209
MS.gene02255.t1	AT4G33720	61.146	157	58	2	3	159	10	163	3.00e-67	202
MS.gene02255.t1	AT2G14610	60.390	154	59	1	6	159	10	161	7.86e-64	193
MS.gene02255.t1	AT3G19690	55.828	163	66	3	1	159	1	161	2.19e-62	189
MS.gene02255.t1	AT5G26130	56.024	166	66	2	1	159	1	166	3.18e-61	186
MS.gene02255.t1	AT4G33710	60.000	140	54	1	22	159	27	166	4.84e-58	178
MS.gene02255.t1	AT1G50060	50.318	157	74	2	4	159	8	161	1.55e-50	159
MS.gene02255.t1	AT4G33730	54.135	133	59	2	28	159	41	172	2.55e-47	151
MS.gene02255.t1	AT2G19990	53.906	128	58	1	33	159	49	176	3.08e-47	151
MS.gene02255.t1	AT4G25790	51.471	136	64	2	26	159	75	210	1.50e-46	151
MS.gene02255.t1	AT5G57625	51.880	133	62	2	29	159	75	207	1.22e-45	148
MS.gene02255.t1	AT1G50050	50.000	158	74	3	4	159	8	162	6.66e-45	145
MS.gene02255.t1	AT3G09590	47.407	135	68	2	28	159	52	186	1.92e-43	142
MS.gene02255.t1	AT4G30320	44.966	149	79	3	13	159	14	161	2.28e-43	141
MS.gene02255.t1	AT4G25780	48.905	137	65	3	28	159	54	190	1.14e-42	140
MS.gene02255.t1	AT1G50050	48.718	156	75	3	4	157	8	160	7.38e-42	139
MS.gene02255.t1	AT4G07820	46.763	139	72	2	21	159	24	160	5.43e-41	135
MS.gene02255.t1	AT1G01310	47.015	134	68	2	28	159	87	219	2.07e-40	136
MS.gene02255.t1	AT5G02730	44.853	136	72	2	27	159	58	193	9.05e-40	133
MS.gene02255.t1	AT4G31470	43.704	135	75	1	26	159	51	185	7.37e-38	128
MS.gene02255.t1	AT5G66590	42.336	137	73	4	29	159	49	185	3.23e-31	111
MS.gene02255.t1	AT2G19970	43.262	141	67	7	30	159	39	177	1.06e-27	102
MS.gene02255.t1	AT2G19980	36.905	168	84	9	4	159	8	165	4.94e-23	89.7

Find 40 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCACTCCGGTGGCCGTTAC+GGG	0.273758	2.1:-72922456	MS.gene02255:CDS
TTGGGCTTATTACTCATTAT+TGG	0.280398	2.1:-72922634	MS.gene02255:CDS
ATCCACTCCGGTGGCCGTTA+CGG	0.326170	2.1:-72922457	MS.gene02255:CDS
GCCATTATACTCAGGTTGTT+TGG	0.328715	2.1:-72922315	MS.gene02255:CDS
CATAGTCTGCTTGTGAGTCT+TGG	0.335837	2.1:+72922596	None:intergenic
TTGGTGTTCAAAATATTGTT+TGG	0.341386	2.1:-72922543	MS.gene02255:CDS
AATAGTAACACATGTGCTTC+TGG	0.347660	2.1:-72922349	MS.gene02255:CDS
TATTAAGCATGCACTTAGTA+TGG	0.353177	2.1:+72922190	None:intergenic
ATCTGAGCCACTCAAGTCTC+CGG	0.382702	2.1:+72922414	None:intergenic
TCCAAACAACCTGAGTATAA+TGG	0.390459	2.1:+72922314	None:intergenic
TGGTGTTCAAAATATTGTTT+GGG	0.400203	2.1:-72922542	MS.gene02255:CDS
ATAGTCTGCTTGTGAGTCTT+GGG	0.401577	2.1:+72922597	None:intergenic
TCTGAGCCACTCAAGTCTCC+GGG	0.459067	2.1:+72922415	None:intergenic
CGTTATTATAGTTGCCTGGT+GGG	0.465282	2.1:+72922218	None:intergenic
TCCATTGTCACATCTCACTT+TGG	0.491133	2.1:+72922267	None:intergenic
CCCCGTAACGGCCACCGGAG+TGG	0.509997	2.1:+72922455	None:intergenic
GAGAATCTCGCGGGTAGCCC+CGG	0.516219	2.1:-72922433	MS.gene02255:CDS
TGGAGAAACTCACAACGTCT+TGG	0.520363	2.1:-72922295	MS.gene02255:CDS
ATTCTCCCCGTAACGGCCAC+CGG	0.528380	2.1:+72922450	None:intergenic
GCTCAGATGCAGTGAAGTTG+TGG	0.543630	2.1:-72922399	MS.gene02255:CDS
CTCAGATGCAGTGAAGTTGT+GGG	0.551141	2.1:-72922398	MS.gene02255:CDS
TCGTTATTATAGTTGCCTGG+TGG	0.573306	2.1:+72922217	None:intergenic
ACTTGCAATTACGACCCACC+AGG	0.586857	2.1:-72922232	MS.gene02255:CDS
GGCCACCGGAGTGGATCAGT+TGG	0.592030	2.1:+72922464	None:intergenic
CCACTCCGGTGGCCGTTACG+GGG	0.597515	2.1:-72922455	MS.gene02255:CDS
CCGCGAGATTCTCCCCGTAA+CGG	0.609234	2.1:+72922443	None:intergenic
CCGTTACGGGGAGAATCTCG+CGG	0.614640	2.1:-72922443	MS.gene02255:CDS
TGCCAACTGATCCACTCCGG+TGG	0.630736	2.1:-72922466	MS.gene02255:CDS
CGTTACGGGGAGAATCTCGC+GGG	0.631764	2.1:-72922442	MS.gene02255:CDS
AACGAAGCAAGATCTGAGGT+TGG	0.637008	2.1:-72922562	MS.gene02255:CDS
AGACTATGCTAATCAACACA+AGG	0.643418	2.1:-72922494	MS.gene02255:CDS
AGTGTGTGGCCATTATACTC+AGG	0.651003	2.1:-72922323	MS.gene02255:CDS
GGTAGCCCCGGAGACTTGAG+TGG	0.652738	2.1:-72922421	MS.gene02255:CDS
GATTGCCAACTGATCCACTC+CGG	0.665515	2.1:-72922469	MS.gene02255:CDS
GACTATGCTAATCAACACAA+GGG	0.683970	2.1:-72922493	MS.gene02255:CDS
TGTGCTTCTGGTAAAGTGTG+TGG	0.690422	2.1:-72922337	MS.gene02255:CDS
TCACAACGAAGCAAGATCTG+AGG	0.699112	2.1:-72922566	MS.gene02255:CDS
GCCAAAGTGAGATGTGACAA+TGG	0.702990	2.1:-72922268	MS.gene02255:CDS
CTGAGCCACTCAAGTCTCCG+GGG	0.708120	2.1:+72922416	None:intergenic
AAAGTGAGATGTGACAATGG+AGG	0.801871	2.1:-72922265	MS.gene02255:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGGTGTTCAAAATATTGTTT+GGG	-	chr2.1:72922323-72922342	MS.gene02255:CDS	25.0%
!!	ATTTTCTCTATTGTGTTTCT+TGG	-	chr2.1:72922212-72922231	MS.gene02255:CDS	25.0%
!!	TTGGTGTTCAAAATATTGTT+TGG	-	chr2.1:72922322-72922341	MS.gene02255:CDS	25.0%
!!	TTTTCTCTATTGTGTTTCTT+GGG	-	chr2.1:72922213-72922232	MS.gene02255:CDS	25.0%
	TTGGGCTTATTACTCATTAT+TGG	-	chr2.1:72922231-72922250	MS.gene02255:CDS	30.0%
!	TTTTCGTTATTATAGTTGCC+TGG	+	chr2.1:72922654-72922673	None:intergenic	30.0%
	AATAGTAACACATGTGCTTC+TGG	-	chr2.1:72922516-72922535	MS.gene02255:CDS	35.0%
	AGACTATGCTAATCAACACA+AGG	-	chr2.1:72922371-72922390	MS.gene02255:CDS	35.0%
	GACTATGCTAATCAACACAA+GGG	-	chr2.1:72922372-72922391	MS.gene02255:CDS	35.0%
	TCCAAACAACCTGAGTATAA+TGG	+	chr2.1:72922554-72922573	None:intergenic	35.0%
	AAAGTGAGATGTGACAATGG+AGG	-	chr2.1:72922600-72922619	MS.gene02255:CDS	40.0%
	ATAGTCTGCTTGTGAGTCTT+GGG	+	chr2.1:72922271-72922290	None:intergenic	40.0%
	CGTTATTATAGTTGCCTGGT+GGG	+	chr2.1:72922650-72922669	None:intergenic	40.0%
	TCCATTGTCACATCTCACTT+TGG	+	chr2.1:72922601-72922620	None:intergenic	40.0%
	TCGTTATTATAGTTGCCTGG+TGG	+	chr2.1:72922651-72922670	None:intergenic	40.0%
!	GCCATTATACTCAGGTTGTT+TGG	-	chr2.1:72922550-72922569	MS.gene02255:CDS	40.0%
	AGTGTGTGGCCATTATACTC+AGG	-	chr2.1:72922542-72922561	MS.gene02255:CDS	45.0%
	CATAGTCTGCTTGTGAGTCT+TGG	+	chr2.1:72922272-72922291	None:intergenic	45.0%
	CTCAGATGCAGTGAAGTTGT+GGG	-	chr2.1:72922467-72922486	MS.gene02255:CDS	45.0%
	GCCAAAGTGAGATGTGACAA+TGG	-	chr2.1:72922597-72922616	MS.gene02255:CDS	45.0%
	TCACAACGAAGCAAGATCTG+AGG	-	chr2.1:72922299-72922318	MS.gene02255:CDS	45.0%
	TGGAGAAACTCACAACGTCT+TGG	-	chr2.1:72922570-72922589	MS.gene02255:CDS	45.0%
	TGTGCTTCTGGTAAAGTGTG+TGG	-	chr2.1:72922528-72922547	MS.gene02255:CDS	45.0%
!	AACGAAGCAAGATCTGAGGT+TGG	-	chr2.1:72922303-72922322	MS.gene02255:CDS	45.0%
	ACTTGCAATTACGACCCACC+AGG	-	chr2.1:72922633-72922652	MS.gene02255:CDS	50.0%
	ATCTGAGCCACTCAAGTCTC+CGG	+	chr2.1:72922454-72922473	None:intergenic	50.0%
	GATTGCCAACTGATCCACTC+CGG	-	chr2.1:72922396-72922415	MS.gene02255:CDS	50.0%
	GCTCAGATGCAGTGAAGTTG+TGG	-	chr2.1:72922466-72922485	MS.gene02255:CDS	50.0%
	TCTGAGCCACTCAAGTCTCC+GGG	+	chr2.1:72922453-72922472	None:intergenic	55.0%
	ATCCACTCCGGTGGCCGTTA+CGG	-	chr2.1:72922408-72922427	MS.gene02255:CDS	60.0%
	ATTCTCCCCGTAACGGCCAC+CGG	+	chr2.1:72922418-72922437	None:intergenic	60.0%
	CCGCGAGATTCTCCCCGTAA+CGG	+	chr2.1:72922425-72922444	None:intergenic	60.0%
	CCGTTACGGGGAGAATCTCG+CGG	-	chr2.1:72922422-72922441	MS.gene02255:CDS	60.0%
	CGTTACGGGGAGAATCTCGC+GGG	-	chr2.1:72922423-72922442	MS.gene02255:CDS	60.0%
	CTGAGCCACTCAAGTCTCCG+GGG	+	chr2.1:72922452-72922471	None:intergenic	60.0%
	TGCCAACTGATCCACTCCGG+TGG	-	chr2.1:72922399-72922418	MS.gene02255:CDS	60.0%
	GAGAATCTCGCGGGTAGCCC+CGG	-	chr2.1:72922432-72922451	MS.gene02255:CDS	65.0%
	GGCCACCGGAGTGGATCAGT+TGG	+	chr2.1:72922404-72922423	None:intergenic	65.0%
	GGTAGCCCCGGAGACTTGAG+TGG	-	chr2.1:72922444-72922463	MS.gene02255:CDS	65.0%
	TCCACTCCGGTGGCCGTTAC+GGG	-	chr2.1:72922409-72922428	MS.gene02255:CDS	65.0%
	CCACTCCGGTGGCCGTTACG+GGG	-	chr2.1:72922410-72922429	MS.gene02255:CDS	70.0%
	CCCCGTAACGGCCACCGGAG+TGG	+	chr2.1:72922413-72922432	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	72922204	72922683	72922204	ID=MS.gene02255
chr2.1	mRNA	72922204	72922683	72922204	ID=MS.gene02255.t1;Parent=MS.gene02255
chr2.1	exon	72922204	72922683	72922204	ID=MS.gene02255.t1.exon1;Parent=MS.gene02255.t1
chr2.1	CDS	72922204	72922683	72922204	ID=cds.MS.gene02255.t1;Parent=MS.gene02255.t1

Gene Sequence

>MS.gene02255

ATGAGTTTATTTTCTCTATTGTGTTTCTTGGGCTTATTACTCATTATTGGTCATGTTGCACATGCCCAAGACTCACAAGCAGACTATGTGAACGCTCACAACGAAGCAAGATCTGAGGTTGGTGTTCAAAATATTGTTTGGGACAATACTGTTGCTAGTTTTGCACAAGACTATGCTAATCAACACAAGGGTGATTGCCAACTGATCCACTCCGGTGGCCGTTACGGGGAGAATCTCGCGGGTAGCCCCGGAGACTTGAGTGGCTCAGATGCAGTGAAGTTGTGGGTGAATGAGAAAGTTGACTATGATTATAATAGTAACACATGTGCTTCTGGTAAAGTGTGTGGCCATTATACTCAGGTTGTTTGGAGAAACTCACAACGTCTTGGATGTGCCAAAGTGAGATGTGACAATGGAGGCACATTCATTACTTGCAATTACGACCCACCAGGCAACTATAATAACGAAAAGCCATACTAA

Protein sequence

>MS.gene02255.t1

MSLFSLLCFLGLLLIIGHVAHAQDSQADYVNAHNEARSEVGVQNIVWDNTVASFAQDYANQHKGDCQLIHSGGRYGENLAGSPGDLSGSDAVKLWVNEKVDYDYNSNTCASGKVCGHYTQVVWRNSQRLGCAKVRCDNGGTFITCNYDPPGNYNNEKPY