AlfalfaGEDB | MS.gene02258

M. sativa cultivar XinJiangDaYe / MS.gene02258

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02258.t1	XP_003593363.1	95.2	146	7	0	3	148	20	165	8.20E-80	306.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02258.t1	Q9ZNS4	62.0	142	47	4	8	148	26	161	3.5e-47	189.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02258.t1	G7IFD6	95.2	146	7	0	3	148	20	165	5.9e-80	306.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene01277	MS.gene02258	PPI
MS.gene01684	MS.gene02258	PPI
MS.gene02255	MS.gene02258	PPI
MS.gene00249	MS.gene02258	PPI
MS.gene02256	MS.gene02258	PPI
MS.gene002297	MS.gene02258	PPI
MS.gene02254	MS.gene02258	PPI
MS.gene01273	MS.gene02258	PPI
MS.gene018353	MS.gene02258	PPI
MS.gene002299	MS.gene02258	PPI
MS.gene01686	MS.gene02258	PPI
MS.gene02258	MS.gene038936	PPI
MS.gene002295	MS.gene02258	PPI
MS.gene57900	MS.gene02258	PPI
MS.gene01272	MS.gene02258	PPI
MS.gene038936	MS.gene02258	PPI
MS.gene01688	MS.gene02258	PPI
MS.gene01356	MS.gene02258	PPI
MS.gene02258	MS.gene002293	PPI
MS.gene002292	MS.gene02258	PPI
MS.gene02258	MS.gene002296	PPI
MS.gene02249	MS.gene02258	PPI
MS.gene02258	MS.gene002297	PPI
MS.gene002294	MS.gene02258	PPI
MS.gene012420	MS.gene02258	PPI
MS.gene02252	MS.gene02258	PPI
MS.gene02258	MS.gene01274	PPI
MS.gene035687	MS.gene02258	PPI
MS.gene01690	MS.gene02258	PPI
MS.gene035685	MS.gene02258	PPI
MS.gene01685	MS.gene02258	PPI
MS.gene01275	MS.gene02258	PPI
MS.gene028545	MS.gene02258	PPI
MS.gene01270	MS.gene02258	PPI
MS.gene01687	MS.gene02258	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02258.t1	MTR_2g010650	95.205	146	7	0	3	148	20	165	6.78e-105	296
MS.gene02258.t1	MTR_2g010630	87.333	150	17	2	1	148	16	165	1.97e-94	270
MS.gene02258.t1	MTR_2g010700	72.078	154	36	4	1	148	16	168	1.82e-75	222
MS.gene02258.t1	MTR_2g010640	69.128	149	44	2	1	148	16	163	4.49e-73	216
MS.gene02258.t1	MTR_2g010670	69.799	149	44	1	1	148	16	164	4.24e-72	213
MS.gene02258.t1	MTR_2g010600	71.141	149	35	5	4	148	18	162	9.87e-72	213
MS.gene02258.t1	MTR_2g010690	66.443	149	49	1	1	148	16	164	1.62e-69	207
MS.gene02258.t1	MTR_4g050762	69.799	149	37	5	4	148	18	162	3.19e-69	207
MS.gene02258.t1	MTR_2g012370	69.799	149	37	5	4	148	18	162	3.19e-69	207
MS.gene02258.t1	MTR_2g010590	65.986	147	43	4	4	148	18	159	4.04e-69	206
MS.gene02258.t1	MTR_2g010610	60.544	147	30	5	4	148	18	138	3.07e-55	170
MS.gene02258.t1	MTR_2g435490	54.362	149	60	5	3	148	21	164	5.71e-53	166
MS.gene02258.t1	MTR_2g010580	53.425	146	62	3	4	148	24	164	6.17e-53	165
MS.gene02258.t1	MTR_4g092540	48.667	150	70	4	1	148	15	159	4.65e-47	150
MS.gene02258.t1	MTR_4g128750	55.072	138	53	6	15	148	41	173	8.64e-40	132
MS.gene02258.t1	MTR_4g128770	48.227	141	63	6	14	148	70	206	2.03e-39	132
MS.gene02258.t1	MTR_1g067290	38.571	140	75	6	14	147	73	207	1.67e-26	99.4
MS.gene02258.t1	MTR_8g078770	35.461	141	81	5	14	148	36	172	1.23e-22	88.2
MS.gene02258.t1	MTR_5g018770	32.867	143	84	5	14	148	47	185	5.22e-21	84.3
MS.gene02258.t1	MTR_4g092515	53.846	78	29	4	6	81	24	96	3.81e-18	75.9
MS.gene02258.t1	MTR_5g018755	35.644	101	58	5	55	148	3	103	1.26e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02258.t1	AT2G14580	61.972	142	47	4	8	148	26	161	7.08e-58	177
MS.gene02258.t1	AT4G33720	59.441	143	51	4	8	148	26	163	7.42e-57	175
MS.gene02258.t1	AT2G14610	58.333	144	53	4	6	148	24	161	1.26e-56	174
MS.gene02258.t1	AT3G19690	57.931	145	52	4	8	148	22	161	2.16e-55	171
MS.gene02258.t1	AT5G26130	58.333	144	53	4	8	148	27	166	4.49e-53	166
MS.gene02258.t1	AT4G33710	56.944	144	55	4	8	148	27	166	6.75e-52	162
MS.gene02258.t1	AT1G50060	58.219	146	53	5	6	148	21	161	8.88e-51	159
MS.gene02258.t1	AT4G33730	52.027	148	64	3	3	148	30	172	1.69e-47	151
MS.gene02258.t1	AT4G25790	48.921	139	62	6	14	148	77	210	4.49e-42	139
MS.gene02258.t1	AT1G50050	52.414	145	62	4	7	148	22	162	8.87e-42	137
MS.gene02258.t1	AT2G19990	49.286	140	63	5	12	148	42	176	1.21e-40	134
MS.gene02258.t1	AT5G57625	49.275	138	61	6	15	148	75	207	1.53e-40	135
MS.gene02258.t1	AT4G25780	48.936	141	62	5	14	148	54	190	6.45e-39	130
MS.gene02258.t1	AT4G07820	45.070	142	71	4	8	148	25	160	1.12e-37	126
MS.gene02258.t1	AT4G30320	46.043	139	71	4	13	148	24	161	2.38e-37	125
MS.gene02258.t1	AT3G09590	47.143	140	64	6	14	148	52	186	3.46e-37	125
MS.gene02258.t1	AT1G50050	52.593	135	57	4	7	138	22	152	3.67e-37	127
MS.gene02258.t1	AT4G31470	42.754	138	71	5	14	148	53	185	2.65e-36	123
MS.gene02258.t1	AT5G02730	47.518	141	64	6	13	148	58	193	6.19e-35	120
MS.gene02258.t1	AT1G01310	44.928	138	68	5	14	148	87	219	3.71e-32	114
MS.gene02258.t1	AT5G66590	36.879	141	78	5	15	148	49	185	5.32e-26	97.4
MS.gene02258.t1	AT2G19980	40.278	144	66	6	12	148	35	165	1.49e-23	90.5
MS.gene02258.t1	AT2G19970	37.584	149	75	7	12	148	35	177	6.30e-22	86.7

Find 37 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTTGTCCCAAACAATATT+TGG	0.198843	2.1:+72914861	None:intergenic
TTAAAATTCCAAATATTGTT+TGG	0.293275	2.1:-72914869	MS.gene02258:CDS
GATTGTCAATTGGTCCATTC+TGG	0.325775	2.1:-72914798	MS.gene02258:CDS
TCCATTATACTCAGGTGGTT+TGG	0.346423	2.1:-72914644	MS.gene02258:CDS
TAAAATTCCAAATATTGTTT+GGG	0.350860	2.1:-72914868	MS.gene02258:CDS
CTGCCGATCTTGCTTGGTTG+TGG	0.358764	2.1:+72914904	None:intergenic
GATCCACCAGGCAACTATAT+TGG	0.377482	2.1:-72914549	MS.gene02258:CDS
CAAATATGAATCACTTAGTA+TGG	0.381615	2.1:+72914519	None:intergenic
TTACTACTATAATCGTAGTT+TGG	0.383101	2.1:+72914690	None:intergenic
TCCAAACCACCTGAGTATAA+TGG	0.384825	2.1:+72914643	None:intergenic
TGCCGATCTTGCTTGGTTGT+GGG	0.426868	2.1:+72914905	None:intergenic
GCCAAAGTGAAATGTGATAA+TGG	0.434324	2.1:-72914597	MS.gene02258:CDS
TATCAACTGCCGATCTTGCT+TGG	0.450687	2.1:+72914898	None:intergenic
ACCATTATCACATTTCACTT+TGG	0.465618	2.1:+72914596	None:intergenic
CATTCTGGTGGTGACCGTTA+CGG	0.493043	2.1:-72914783	MS.gene02258:CDS
TCAACGCAAGGATTGTCAAT+TGG	0.501362	2.1:-72914808	MS.gene02258:CDS
CTTTCGCCAATATAGTTGCC+TGG	0.506506	2.1:+72914543	None:intergenic
TGCCCACAACCAAGCAAGAT+CGG	0.521741	2.1:-72914907	MS.gene02258:CDS
TGGGCATTCACGTAGTCTGC+TGG	0.533658	2.1:+72914924	None:intergenic
GAGAATATCGCGGAGAGCAG+CGG	0.550660	2.1:-72914759	MS.gene02258:CDS
CGTAACGGTCACCACCAGAA+TGG	0.554171	2.1:+72914784	None:intergenic
GTATAGAAGCAGTGAAGTTG+TGG	0.558270	2.1:-72914725	MS.gene02258:CDS
TATAGTAGTAACTCATGTGT+TGG	0.568160	2.1:-72914678	MS.gene02258:CDS
TGTCAATTGGTCCATTCTGG+TGG	0.605326	2.1:-72914795	MS.gene02258:CDS
TATAGAAGCAGTGAAGTTGT+GGG	0.617285	2.1:-72914724	MS.gene02258:CDS
AAATTATGCTAATCAACGCA+AGG	0.622269	2.1:-72914820	MS.gene02258:CDS
CCGCGATATTCTCTCCGTAA+CGG	0.622517	2.1:+72914769	None:intergenic
ATGCCTCCATTATACTCAGG+TGG	0.625424	2.1:-72914649	MS.gene02258:CDS
GAGAGCAGCGGCGACATGAG+TGG	0.630427	2.1:-72914747	MS.gene02258:CDS
AGAAGCAGTGAAGTTGTGGG+TGG	0.644949	2.1:-72914721	MS.gene02258:CDS
TGAATGCCTCCATTATACTC+AGG	0.648037	2.1:-72914652	MS.gene02258:CDS
ACTTGCAATTACGATCCACC+AGG	0.648548	2.1:-72914561	MS.gene02258:CDS
TCGCCAATATAGTTGCCTGG+TGG	0.651539	2.1:+72914546	None:intergenic
AGTAGTAACTCATGTGTTGG+TGG	0.657995	2.1:-72914675	MS.gene02258:CDS
AAACCACCTGAGTATAATGG+AGG	0.693168	2.1:+72914646	None:intergenic
CCGTTACGGAGAGAATATCG+CGG	0.753101	2.1:-72914769	MS.gene02258:CDS
AAAGTGAAATGTGATAATGG+TGG	0.767769	2.1:-72914594	MS.gene02258:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATTCCAAATATTGTTT+GGG	-	chr2.1:72914622-72914641	MS.gene02258:CDS	15.0%
!!	TTAAAATTCCAAATATTGTT+TGG	-	chr2.1:72914621-72914640	MS.gene02258:CDS	15.0%
!	TTACTACTATAATCGTAGTT+TGG	+	chr2.1:72914803-72914822	None:intergenic	25.0%
	AAATTATGCTAATCAACGCA+AGG	-	chr2.1:72914670-72914689	MS.gene02258:CDS	30.0%
	ACCATTATCACATTTCACTT+TGG	+	chr2.1:72914897-72914916	None:intergenic	30.0%
	TATAGTAGTAACTCATGTGT+TGG	-	chr2.1:72914812-72914831	MS.gene02258:CDS	30.0%
	TTGTTGTCCCAAACAATATT+TGG	+	chr2.1:72914632-72914651	None:intergenic	30.0%
!!	AAAGTGAAATGTGATAATGG+TGG	-	chr2.1:72914896-72914915	MS.gene02258:CDS	30.0%
	GCCAAAGTGAAATGTGATAA+TGG	-	chr2.1:72914893-72914912	MS.gene02258:CDS	35.0%
	TATAGAAGCAGTGAAGTTGT+GGG	-	chr2.1:72914766-72914785	MS.gene02258:CDS	35.0%
	TGGAGAAAAACACAACGTAT+TGG	-	chr2.1:72914866-72914885	MS.gene02258:CDS	35.0%
	AAACCACCTGAGTATAATGG+AGG	+	chr2.1:72914847-72914866	None:intergenic	40.0%
	AGTAGTAACTCATGTGTTGG+TGG	-	chr2.1:72914815-72914834	MS.gene02258:CDS	40.0%
	GATTGTCAATTGGTCCATTC+TGG	-	chr2.1:72914692-72914711	MS.gene02258:CDS	40.0%
	GTATAGAAGCAGTGAAGTTG+TGG	-	chr2.1:72914765-72914784	MS.gene02258:CDS	40.0%
	TCAACGCAAGGATTGTCAAT+TGG	-	chr2.1:72914682-72914701	MS.gene02258:CDS	40.0%
	TCCAAACCACCTGAGTATAA+TGG	+	chr2.1:72914850-72914869	None:intergenic	40.0%
	TCCATTATACTCAGGTGGTT+TGG	-	chr2.1:72914846-72914865	MS.gene02258:CDS	40.0%
	TGAATGCCTCCATTATACTC+AGG	-	chr2.1:72914838-72914857	MS.gene02258:CDS	40.0%
	ACTTGCAATTACGATCCACC+AGG	-	chr2.1:72914929-72914948	MS.gene02258:CDS	45.0%
	ATGCCTCCATTATACTCAGG+TGG	-	chr2.1:72914841-72914860	MS.gene02258:CDS	45.0%
	CTTTCGCCAATATAGTTGCC+TGG	+	chr2.1:72914950-72914969	None:intergenic	45.0%
	GATCCACCAGGCAACTATAT+TGG	-	chr2.1:72914941-72914960	MS.gene02258:CDS	45.0%
	TATCAACTGCCGATCTTGCT+TGG	+	chr2.1:72914595-72914614	None:intergenic	45.0%
	TGTCAATTGGTCCATTCTGG+TGG	-	chr2.1:72914695-72914714	MS.gene02258:CDS	45.0%
	AGAAGCAGTGAAGTTGTGGG+TGG	-	chr2.1:72914769-72914788	MS.gene02258:CDS	50.0%
	CCGCGATATTCTCTCCGTAA+CGG	+	chr2.1:72914724-72914743	None:intergenic	50.0%
	CCGTTACGGAGAGAATATCG+CGG	-	chr2.1:72914721-72914740	MS.gene02258:CDS	50.0%
	TCGCCAATATAGTTGCCTGG+TGG	+	chr2.1:72914947-72914966	None:intergenic	50.0%
	TGCCCACAACCAAGCAAGAT+CGG	-	chr2.1:72914583-72914602	MS.gene02258:CDS	50.0%
	TGCCGATCTTGCTTGGTTGT+GGG	+	chr2.1:72914588-72914607	None:intergenic	50.0%
!	CATTCTGGTGGTGACCGTTA+CGG	-	chr2.1:72914707-72914726	MS.gene02258:CDS	50.0%
	CGTAACGGTCACCACCAGAA+TGG	+	chr2.1:72914709-72914728	None:intergenic	55.0%
	CTGCCGATCTTGCTTGGTTG+TGG	+	chr2.1:72914589-72914608	None:intergenic	55.0%
	GAGAATATCGCGGAGAGCAG+CGG	-	chr2.1:72914731-72914750	MS.gene02258:CDS	55.0%
	TGGGCATTCACGTAGTCTGC+TGG	+	chr2.1:72914569-72914588	None:intergenic	55.0%
	GAGAGCAGCGGCGACATGAG+TGG	-	chr2.1:72914743-72914762	MS.gene02258:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	72914533	72914979	72914533	ID=MS.gene02258
chr2.1	mRNA	72914533	72914979	72914533	ID=MS.gene02258.t1;Parent=MS.gene02258
chr2.1	exon	72914533	72914979	72914533	ID=MS.gene02258.t1.exon1;Parent=MS.gene02258.t1
chr2.1	CDS	72914533	72914979	72914533	ID=cds.MS.gene02258.t1;Parent=MS.gene02258.t1

Gene Sequence

>MS.gene02258

ATGGATAGTCACGTTGCACATGCACAAGACTCACCAGCAGACTACGTGAATGCCCACAACCAAGCAAGATCGGCAGTTGATACAAACATTAAAATTCCAAATATTGTTTGGGACAACAACGTCGCTGCTTTTGCACAAAATTATGCTAATCAACGCAAGGATTGTCAATTGGTCCATTCTGGTGGTGACCGTTACGGAGAGAATATCGCGGAGAGCAGCGGCGACATGAGTGGTATAGAAGCAGTGAAGTTGTGGGTGGATGAAAAACCAAACTACGATTATAGTAGTAACTCATGTGTTGGTGGTGAATGCCTCCATTATACTCAGGTGGTTTGGAGAAAAACACAACGTATTGGATGTGCCAAAGTGAAATGTGATAATGGTGGCACATTTGTTACTTGCAATTACGATCCACCAGGCAACTATATTGGCGAAAGACCATACTAA

Protein sequence

>MS.gene02258.t1

MDSHVAHAQDSPADYVNAHNQARSAVDTNIKIPNIVWDNNVAAFAQNYANQRKDCQLVHSGGDRYGENIAESSGDMSGIEAVKLWVDEKPNYDYSSNSCVGGECLHYTQVVWRKTQRIGCAKVKCDNGGTFVTCNYDPPGNYIGERPY