AlfalfaGEDB | MS.gene002299

M. sativa cultivar XinJiangDaYe / MS.gene002299

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002299.t1	XP_003593363.1	95.2	146	7	0	3	148	20	165	8.20E-80	306.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002299.t1	Q9ZNS4	62.7	142	46	4	8	148	26	161	1.2e-47	190.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002299.t1	G7IFD6	95.2	146	7	0	3	148	20	165	5.9e-80	306.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene01273	MS.gene002299	PPI
MS.gene002299	MS.gene01356	PPI
MS.gene002299	MS.gene01272	PPI
MS.gene002299	MS.gene01277	PPI
MS.gene002299	MS.gene01688	PPI
MS.gene002299	MS.gene02256	PPI
MS.gene002299	MS.gene01690	PPI
MS.gene002299	MS.gene02255	PPI
MS.gene002299	MS.gene01686	PPI
MS.gene002299	MS.gene01685	PPI
MS.gene002299	MS.gene002295	PPI
MS.gene002299	MS.gene035687	PPI
MS.gene01688	MS.gene002299	PPI
MS.gene002299	MS.gene002294	PPI
MS.gene002299	MS.gene01687	PPI
MS.gene002299	MS.gene01275	PPI
MS.gene002299	MS.gene01273	PPI
MS.gene002299	MS.gene02258	PPI
MS.gene002299	MS.gene002292	PPI
MS.gene01687	MS.gene002299	PPI
MS.gene002299	MS.gene012420	PPI
MS.gene002299	MS.gene02254	PPI
MS.gene002299	MS.gene035685	PPI
MS.gene002299	MS.gene01684	PPI
MS.gene002299	MS.gene038936	PPI
MS.gene028545	MS.gene002299	PPI
MS.gene002299	MS.gene57900	PPI
MS.gene002299	MS.gene00249	PPI
MS.gene002299	MS.gene02252	PPI
MS.gene002299	MS.gene002293	PPI
MS.gene002299	MS.gene01270	PPI
MS.gene002299	MS.gene02249	PPI
MS.gene002299	MS.gene002296	PPI
MS.gene002299	MS.gene002297	PPI
MS.gene002299	MS.gene018353	PPI
MS.gene002299	MS.gene01274	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002299.t1	MTR_2g010650	95.205	146	7	0	3	148	20	165	1.46e-104	295
MS.gene002299.t1	MTR_2g010630	87.333	150	17	2	1	148	16	165	3.80e-94	269
MS.gene002299.t1	MTR_2g010700	70.779	154	38	4	1	148	16	168	1.47e-73	217
MS.gene002299.t1	MTR_2g010600	71.141	149	35	5	4	148	18	162	1.67e-71	212
MS.gene002299.t1	MTR_2g010640	67.785	149	46	2	1	148	16	163	1.97e-71	212
MS.gene002299.t1	MTR_2g010670	69.128	149	45	1	1	148	16	164	2.95e-71	211
MS.gene002299.t1	MTR_4g050762	69.799	149	37	5	4	148	18	162	4.73e-69	206
MS.gene002299.t1	MTR_2g012370	69.799	149	37	5	4	148	18	162	4.73e-69	206
MS.gene002299.t1	MTR_2g010590	65.986	147	43	4	4	148	18	159	6.90e-69	205
MS.gene002299.t1	MTR_2g010690	65.772	149	50	1	1	148	16	164	8.68e-69	205
MS.gene002299.t1	MTR_2g010610	61.224	147	29	5	4	148	18	138	6.15e-56	172
MS.gene002299.t1	MTR_2g435490	54.362	149	60	5	3	148	21	164	3.65e-53	166
MS.gene002299.t1	MTR_2g010580	53.103	145	62	3	5	148	25	164	3.29e-52	163
MS.gene002299.t1	MTR_4g092540	50.000	150	68	4	1	148	15	159	7.26e-49	154
MS.gene002299.t1	MTR_4g128750	54.348	138	54	6	15	148	41	173	7.29e-39	129
MS.gene002299.t1	MTR_4g128770	47.518	141	64	6	14	148	70	206	9.80e-39	130
MS.gene002299.t1	MTR_1g067290	37.857	140	76	6	14	147	73	207	4.25e-26	98.2
MS.gene002299.t1	MTR_8g078770	34.752	141	82	5	14	148	36	172	7.55e-22	86.3
MS.gene002299.t1	MTR_5g018770	32.168	143	85	5	14	148	47	185	6.34e-20	81.6
MS.gene002299.t1	MTR_4g092515	52.564	78	30	4	6	81	24	96	4.13e-17	73.2
MS.gene002299.t1	MTR_5g018755	34.653	101	59	5	55	148	3	103	6.35e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002299.t1	AT2G14580	62.676	142	46	4	8	148	26	161	1.47e-58	179
MS.gene002299.t1	AT4G33720	59.441	143	51	4	8	148	26	163	8.28e-57	175
MS.gene002299.t1	AT2G14610	56.944	144	55	4	6	148	24	161	2.18e-55	171
MS.gene002299.t1	AT3G19690	57.931	145	52	4	8	148	22	161	3.46e-55	171
MS.gene002299.t1	AT5G26130	57.639	144	54	4	8	148	27	166	3.04e-52	163
MS.gene002299.t1	AT4G33710	56.944	144	55	4	8	148	27	166	5.92e-52	162
MS.gene002299.t1	AT1G50060	57.534	146	54	5	6	148	21	161	6.70e-50	157
MS.gene002299.t1	AT4G33730	52.027	148	64	3	3	148	30	172	2.54e-47	151
MS.gene002299.t1	AT1G50050	52.414	145	62	4	7	148	22	162	1.55e-41	136
MS.gene002299.t1	AT4G25790	48.201	139	63	6	14	148	77	210	5.05e-41	136
MS.gene002299.t1	AT2G19990	48.571	140	64	5	12	148	42	176	4.86e-40	132
MS.gene002299.t1	AT5G57625	48.551	138	62	6	15	148	75	207	5.89e-40	133
MS.gene002299.t1	AT4G25780	48.227	141	63	5	14	148	54	190	3.43e-38	128
MS.gene002299.t1	AT4G30320	46.043	139	71	4	13	148	24	161	2.36e-37	125
MS.gene002299.t1	AT1G50050	52.593	135	57	4	7	138	22	152	4.81e-37	127
MS.gene002299.t1	AT3G09590	46.429	140	65	6	14	148	52	186	2.39e-36	124
MS.gene002299.t1	AT4G07820	43.357	143	74	4	7	148	24	160	3.05e-36	122
MS.gene002299.t1	AT4G31470	41.304	138	73	5	14	148	53	185	7.56e-35	120
MS.gene002299.t1	AT5G02730	46.809	141	65	6	13	148	58	193	2.69e-34	119
MS.gene002299.t1	AT1G01310	44.203	138	69	5	14	148	87	219	1.47e-31	113
MS.gene002299.t1	AT5G66590	37.589	141	77	5	15	148	49	185	5.48e-27	99.8
MS.gene002299.t1	AT2G19980	39.583	144	67	6	12	148	35	165	1.10e-22	88.2
MS.gene002299.t1	AT2G19970	37.584	149	75	7	12	148	35	177	1.19e-21	85.9

Find 37 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTTGTCCCAAACAATATT+TGG	0.198843	2.3:+72736955	None:intergenic
TTAAAATTCCAAATATTGTT+TGG	0.293275	2.3:-72736963	MS.gene002299:CDS
GATTGTCAATTGGTCCATTC+TGG	0.325775	2.3:-72736892	MS.gene002299:CDS
TCCATTATACTCAGGTGGTT+TGG	0.346423	2.3:-72736738	MS.gene002299:CDS
TAAAATTCCAAATATTGTTT+GGG	0.350860	2.3:-72736962	MS.gene002299:CDS
CTGCCGATCTTGCTTGGTTG+TGG	0.358764	2.3:+72736998	None:intergenic
GATCCACCAGGCAACTATAT+TGG	0.377482	2.3:-72736643	MS.gene002299:CDS
TTACTACTATAATCGTAGTT+TGG	0.383101	2.3:+72736784	None:intergenic
TCCAAACCACCTGAGTATAA+TGG	0.384825	2.3:+72736737	None:intergenic
TGCCGATCTTGCTTGGTTGT+GGG	0.426868	2.3:+72736999	None:intergenic
GCCAAAGTGAAATGTGATAA+TGG	0.434324	2.3:-72736691	MS.gene002299:CDS
TATCAACTGCCGATCTTGCT+TGG	0.450687	2.3:+72736992	None:intergenic
TGGAGAAACACACAACGTAT+TGG	0.459583	2.3:-72736718	MS.gene002299:CDS
ACCATTATCACATTTCACTT+TGG	0.465618	2.3:+72736690	None:intergenic
ACTCATGTCGCCGCTGCTCT+CGG	0.470748	2.3:+72736843	None:intergenic
TCAACACAAGGATTGTCAAT+TGG	0.491000	2.3:-72736902	MS.gene002299:CDS
CATTCTGGTGGTGACCGTTA+CGG	0.493043	2.3:-72736877	MS.gene002299:CDS
GCATAGAAGCAGTGAAGTTG+TGG	0.504180	2.3:-72736819	MS.gene002299:CDS
CTTTCGCCAATATAGTTGCC+TGG	0.506506	2.3:+72736637	None:intergenic
TGCCCACAACCAAGCAAGAT+CGG	0.521741	2.3:-72737001	MS.gene002299:CDS
TGGGCATTCACGTAGTCTGC+TGG	0.533658	2.3:+72737018	None:intergenic
CAAATATGAATCACTTAGTA+CGG	0.537712	2.3:+72736613	None:intergenic
GAGAATATCGCCGAGAGCAG+CGG	0.550660	2.3:-72736853	MS.gene002299:CDS
CGTAACGGTCACCACCAGAA+TGG	0.554171	2.3:+72736878	None:intergenic
TATAGTAGTAACTCATGTGT+TGG	0.568160	2.3:-72736772	MS.gene002299:CDS
CATAGAAGCAGTGAAGTTGT+GGG	0.603029	2.3:-72736818	MS.gene002299:CDS
TGTCAATTGGTCCATTCTGG+TGG	0.605326	2.3:-72736889	MS.gene002299:CDS
GAGAGCAGCGGCGACATGAG+TGG	0.607916	2.3:-72736841	MS.gene002299:CDS
CGGCGATATTCTCTCCGTAA+CGG	0.622517	2.3:+72736863	None:intergenic
ATGCCTCCATTATACTCAGG+TGG	0.625424	2.3:-72736743	MS.gene002299:CDS
TGAATGCCTCCATTATACTC+AGG	0.648037	2.3:-72736746	MS.gene002299:CDS
ACTTGCAATTACGATCCACC+AGG	0.648548	2.3:-72736655	MS.gene002299:CDS
TCGCCAATATAGTTGCCTGG+TGG	0.651539	2.3:+72736640	None:intergenic
AGTAGTAACTCATGTGTTGG+TGG	0.657995	2.3:-72736769	MS.gene002299:CDS
AAACCACCTGAGTATAATGG+AGG	0.693168	2.3:+72736740	None:intergenic
AAATTATGCTAATCAACACA+AGG	0.695188	2.3:-72736914	MS.gene002299:CDS
AAAGTGAAATGTGATAATGG+TGG	0.767769	2.3:-72736688	MS.gene002299:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATTCCAAATATTGTTT+GGG	-	chr2.3:72736716-72736735	MS.gene002299:CDS	15.0%
!!	TTAAAATTCCAAATATTGTT+TGG	-	chr2.3:72736715-72736734	MS.gene002299:CDS	15.0%
!	AAATTATGCTAATCAACACA+AGG	-	chr2.3:72736764-72736783	MS.gene002299:CDS	25.0%
!	TTACTACTATAATCGTAGTT+TGG	+	chr2.3:72736897-72736916	None:intergenic	25.0%
	ACCATTATCACATTTCACTT+TGG	+	chr2.3:72736991-72737010	None:intergenic	30.0%
	TATAGTAGTAACTCATGTGT+TGG	-	chr2.3:72736906-72736925	MS.gene002299:CDS	30.0%
	TTGTTGTCCCAAACAATATT+TGG	+	chr2.3:72736726-72736745	None:intergenic	30.0%
!!	AAAGTGAAATGTGATAATGG+TGG	-	chr2.3:72736990-72737009	MS.gene002299:CDS	30.0%
	GCCAAAGTGAAATGTGATAA+TGG	-	chr2.3:72736987-72737006	MS.gene002299:CDS	35.0%
	TCAACACAAGGATTGTCAAT+TGG	-	chr2.3:72736776-72736795	MS.gene002299:CDS	35.0%
	AAACCACCTGAGTATAATGG+AGG	+	chr2.3:72736941-72736960	None:intergenic	40.0%
	AGTAGTAACTCATGTGTTGG+TGG	-	chr2.3:72736909-72736928	MS.gene002299:CDS	40.0%
	CATAGAAGCAGTGAAGTTGT+GGG	-	chr2.3:72736860-72736879	MS.gene002299:CDS	40.0%
	GATTGTCAATTGGTCCATTC+TGG	-	chr2.3:72736786-72736805	MS.gene002299:CDS	40.0%
	TCCAAACCACCTGAGTATAA+TGG	+	chr2.3:72736944-72736963	None:intergenic	40.0%
	TCCATTATACTCAGGTGGTT+TGG	-	chr2.3:72736940-72736959	MS.gene002299:CDS	40.0%
	TGAATGCCTCCATTATACTC+AGG	-	chr2.3:72736932-72736951	MS.gene002299:CDS	40.0%
	TGGAGAAACACACAACGTAT+TGG	-	chr2.3:72736960-72736979	MS.gene002299:CDS	40.0%
	ACTTGCAATTACGATCCACC+AGG	-	chr2.3:72737023-72737042	MS.gene002299:CDS	45.0%
	ATGCCTCCATTATACTCAGG+TGG	-	chr2.3:72736935-72736954	MS.gene002299:CDS	45.0%
	CTTTCGCCAATATAGTTGCC+TGG	+	chr2.3:72737044-72737063	None:intergenic	45.0%
	GATCCACCAGGCAACTATAT+TGG	-	chr2.3:72737035-72737054	MS.gene002299:CDS	45.0%
	GCATAGAAGCAGTGAAGTTG+TGG	-	chr2.3:72736859-72736878	MS.gene002299:CDS	45.0%
	TATCAACTGCCGATCTTGCT+TGG	+	chr2.3:72736689-72736708	None:intergenic	45.0%
	TGTCAATTGGTCCATTCTGG+TGG	-	chr2.3:72736789-72736808	MS.gene002299:CDS	45.0%
	CGGCGATATTCTCTCCGTAA+CGG	+	chr2.3:72736818-72736837	None:intergenic	50.0%
	TCGCCAATATAGTTGCCTGG+TGG	+	chr2.3:72737041-72737060	None:intergenic	50.0%
	TGCCCACAACCAAGCAAGAT+CGG	-	chr2.3:72736677-72736696	MS.gene002299:CDS	50.0%
	TGCCGATCTTGCTTGGTTGT+GGG	+	chr2.3:72736682-72736701	None:intergenic	50.0%
!	CATTCTGGTGGTGACCGTTA+CGG	-	chr2.3:72736801-72736820	MS.gene002299:CDS	50.0%
	CGTAACGGTCACCACCAGAA+TGG	+	chr2.3:72736803-72736822	None:intergenic	55.0%
	CTGCCGATCTTGCTTGGTTG+TGG	+	chr2.3:72736683-72736702	None:intergenic	55.0%
	GAGAATATCGCCGAGAGCAG+CGG	-	chr2.3:72736825-72736844	MS.gene002299:CDS	55.0%
	TGGGCATTCACGTAGTCTGC+TGG	+	chr2.3:72736663-72736682	None:intergenic	55.0%
	ACTCATGTCGCCGCTGCTCT+CGG	+	chr2.3:72736838-72736857	None:intergenic	60.0%
	GAGAGCAGCGGCGACATGAG+TGG	-	chr2.3:72736837-72736856	MS.gene002299:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	72736627	72737073	72736627	ID=MS.gene002299
chr2.3	mRNA	72736627	72737073	72736627	ID=MS.gene002299.t1;Parent=MS.gene002299
chr2.3	exon	72736627	72737073	72736627	ID=MS.gene002299.t1.exon1;Parent=MS.gene002299.t1
chr2.3	CDS	72736627	72737073	72736627	ID=cds.MS.gene002299.t1;Parent=MS.gene002299.t1

Gene Sequence

>MS.gene002299

ATGGATAGTCACGTTGCACATGCACAAGACTCACCAGCAGACTACGTGAATGCCCACAACCAAGCAAGATCGGCAGTTGATACAAACATTAAAATTCCAAATATTGTTTGGGACAACAACGTCGCTGCTTTTGCACAAAATTATGCTAATCAACACAAGGATTGTCAATTGGTCCATTCTGGTGGTGACCGTTACGGAGAGAATATCGCCGAGAGCAGCGGCGACATGAGTGGCATAGAAGCAGTGAAGTTGTGGGTTGATGAAAAACCAAACTACGATTATAGTAGTAACTCATGTGTTGGTGGTGAATGCCTCCATTATACTCAGGTGGTTTGGAGAAACACACAACGTATTGGATGTGCCAAAGTGAAATGTGATAATGGTGGCACATTTGTTACTTGCAATTACGATCCACCAGGCAACTATATTGGCGAAAGACCGTACTAA

Protein sequence

>MS.gene002299.t1

MDSHVAHAQDSPADYVNAHNQARSAVDTNIKIPNIVWDNNVAAFAQNYANQHKDCQLVHSGGDRYGENIAESSGDMSGIEAVKLWVDEKPNYDYSSNSCVGGECLHYTQVVWRNTQRIGCAKVKCDNGGTFVTCNYDPPGNYIGERPY