AlfalfaGEDB | MS.gene02252

M. sativa cultivar XinJiangDaYe / MS.gene02252

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02252.t1	RHN71757.1	86.4	162	22	0	1	162	274	435	3.30E-82	314.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02252.t1	P09042	59.5	163	62	3	1	162	9	168	1.8e-52	206.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02252.t1	G7IFD1	86.4	162	22	0	1	162	1	162	2.4e-82	314.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene02249	MS.gene02252	PPI
MS.gene01275	MS.gene02252	PPI
MS.gene02252	MS.gene57900	PPI
MS.gene01687	MS.gene02252	PPI
MS.gene02252	MS.gene01270	PPI
MS.gene035687	MS.gene02252	PPI
MS.gene02252	MS.gene002296	PPI
MS.gene01690	MS.gene02252	PPI
MS.gene02252	MS.gene002297	PPI
MS.gene01684	MS.gene02252	PPI
MS.gene02252	MS.gene02256	PPI
MS.gene028545	MS.gene02252	PPI
MS.gene02252	MS.gene002295	PPI
MS.gene02252	MS.gene002294	PPI
MS.gene02252	MS.gene01274	PPI
MS.gene02254	MS.gene02252	PPI
MS.gene02252	MS.gene002292	PPI
MS.gene02252	MS.gene01277	PPI
MS.gene02255	MS.gene02252	PPI
MS.gene02252	MS.gene01686	PPI
MS.gene00249	MS.gene02252	PPI
MS.gene01272	MS.gene02252	PPI
MS.gene02256	MS.gene02252	PPI
MS.gene02252	MS.gene01685	PPI
MS.gene01688	MS.gene02252	PPI
MS.gene01356	MS.gene02252	PPI
MS.gene01273	MS.gene02252	PPI
MS.gene018353	MS.gene02252	PPI
MS.gene02252	MS.gene02258	PPI
MS.gene002299	MS.gene02252	PPI
MS.gene01686	MS.gene02252	PPI
MS.gene02252	MS.gene038936	PPI
MS.gene012420	MS.gene02252	PPI
MS.gene002295	MS.gene02252	PPI
MS.gene02252	MS.gene002293	PPI
MS.gene035685	MS.gene02252	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02252.t1	MTR_2g010600	86.420	162	22	0	1	162	1	162	2.48e-108	306
MS.gene02252.t1	MTR_4g050762	84.568	162	25	0	1	162	1	162	5.87e-100	285
MS.gene02252.t1	MTR_2g012370	84.568	162	25	0	1	162	1	162	5.87e-100	285
MS.gene02252.t1	MTR_2g010590	84.568	162	22	2	1	162	1	159	2.95e-99	283
MS.gene02252.t1	MTR_2g010650	67.456	169	44	7	1	162	1	165	8.70e-77	226
MS.gene02252.t1	MTR_2g010630	70.779	154	38	4	14	162	14	165	2.87e-76	224
MS.gene02252.t1	MTR_2g010610	70.370	162	24	3	1	162	1	138	1.89e-72	214
MS.gene02252.t1	MTR_2g010700	60.000	170	58	5	1	162	1	168	2.96e-68	204
MS.gene02252.t1	MTR_2g435490	54.938	162	71	1	1	162	5	164	1.09e-66	201
MS.gene02252.t1	MTR_2g010640	59.036	166	61	5	1	162	1	163	1.06e-64	195
MS.gene02252.t1	MTR_2g010670	58.683	167	61	5	1	162	1	164	4.36e-60	184
MS.gene02252.t1	MTR_2g010690	55.090	167	67	5	1	162	1	164	5.61e-57	176
MS.gene02252.t1	MTR_2g010580	44.311	167	84	4	1	162	2	164	7.91e-51	160
MS.gene02252.t1	MTR_4g092540	46.012	163	83	4	1	162	1	159	1.61e-48	154
MS.gene02252.t1	MTR_4g128750	51.111	135	63	2	29	162	41	173	9.76e-45	145
MS.gene02252.t1	MTR_4g128770	45.652	138	71	3	28	162	70	206	3.01e-41	137
MS.gene02252.t1	MTR_1g067290	38.686	137	79	4	28	161	73	207	2.11e-32	115
MS.gene02252.t1	MTR_8g078770	37.681	138	82	4	28	162	36	172	1.43e-26	99.0
MS.gene02252.t1	MTR_5g018770	33.813	139	88	3	28	162	47	185	1.06e-24	94.4
MS.gene02252.t1	MTR_4g092515	50.000	108	47	3	1	103	1	106	2.89e-24	92.0
MS.gene02252.t1	MTR_5g018755	30.851	94	60	3	74	162	10	103	5.68e-13	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02252.t1	AT4G33720	59.627	161	62	2	2	162	6	163	3.23e-67	202
MS.gene02252.t1	AT2G14580	60.976	164	57	4	1	162	3	161	2.36e-65	197
MS.gene02252.t1	AT5G26130	57.962	157	65	1	6	162	11	166	8.24e-64	193
MS.gene02252.t1	AT2G14610	59.236	157	59	3	6	162	10	161	1.30e-61	187
MS.gene02252.t1	AT4G33710	61.702	141	53	1	22	162	27	166	1.87e-59	182
MS.gene02252.t1	AT3G19690	53.459	159	67	4	6	162	8	161	3.46e-57	176
MS.gene02252.t1	AT4G25790	53.676	136	60	2	28	162	77	210	1.63e-50	161
MS.gene02252.t1	AT1G50060	51.807	166	71	5	1	162	1	161	7.81e-49	155
MS.gene02252.t1	AT5G57625	52.593	135	61	2	29	162	75	207	9.75e-49	156
MS.gene02252.t1	AT2G19990	49.635	137	67	1	26	162	42	176	2.09e-48	154
MS.gene02252.t1	AT3G09590	48.905	137	66	2	28	162	52	186	3.61e-45	147
MS.gene02252.t1	AT4G25780	50.725	138	64	3	28	162	54	190	4.79e-45	146
MS.gene02252.t1	AT4G30320	45.695	151	78	3	13	162	14	161	1.30e-44	144
MS.gene02252.t1	AT1G50050	53.614	166	69	5	1	162	1	162	1.78e-44	144
MS.gene02252.t1	AT4G33730	52.174	138	61	4	26	162	39	172	2.40e-44	144
MS.gene02252.t1	AT5G02730	45.985	137	70	2	28	162	59	193	1.74e-40	135
MS.gene02252.t1	AT1G50050	52.564	156	69	4	6	160	9	160	2.44e-39	133
MS.gene02252.t1	AT1G01310	45.588	136	70	3	28	162	87	219	9.36e-39	132
MS.gene02252.t1	AT4G31470	46.324	136	69	3	28	162	53	185	2.35e-38	129
MS.gene02252.t1	AT5G66590	45.000	140	68	3	29	162	49	185	4.86e-37	126
MS.gene02252.t1	AT4G07820	43.662	142	75	4	21	162	24	160	5.52e-36	122
MS.gene02252.t1	AT2G19970	38.971	136	70	6	26	152	35	166	3.80e-24	92.8
MS.gene02252.t1	AT2G19980	34.706	170	90	8	3	162	7	165	4.67e-23	89.7

Find 41 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATAGTCTGTTGGTGAGTTT+TGG	0.180317	2.1:+72942409	None:intergenic
CCGGTGGTGGTGGCCGTTAC+GGG	0.193193	2.1:-72942263	MS.gene02252:CDS
TTAGGCTTATTTCTCATTAT+TGG	0.210544	2.1:-72942447	MS.gene02252:CDS
TCCGGTGGTGGTGGCCGTTA+CGG	0.303334	2.1:-72942264	MS.gene02252:CDS
TATTAATCATGCACTTAGTA+TGG	0.322170	2.1:+72941994	None:intergenic
TTGGTGTTGCAAATGTTGTT+TGG	0.348141	2.1:-72942356	MS.gene02252:CDS
GCCATTATACTCAGGTGGTT+TGG	0.358578	2.1:-72942122	MS.gene02252:CDS
GTTACTATTATAGTTATAAT+CGG	0.390895	2.1:+72942170	None:intergenic
TCCAAACCACCTGAGTATAA+TGG	0.398812	2.1:+72942121	None:intergenic
TGGTGTTGCAAATGTTGTTT+GGG	0.411343	2.1:-72942355	MS.gene02252:CDS
CTGACGTCTTGCTTCGTTGT+GGG	0.413555	2.1:+72942380	None:intergenic
CCCGTAACGGCCACCACCAC+CGG	0.429882	2.1:+72942263	None:intergenic
TGGAGAAACACAAAGCGTAT+TGG	0.455942	2.1:-72942102	MS.gene02252:CDS
GCACAGATGCAGTGAGGTTG+TGG	0.462999	2.1:-72942206	MS.gene02252:CDS
ATTATTATCACATTTCACTT+TGG	0.463790	2.1:+72942074	None:intergenic
TTGATCCACTCCGGTGGTGG+TGG	0.492178	2.1:-72942273	MS.gene02252:CDS
GTGAAATGTGATAATAATAG+AGG	0.492513	2.1:-72942069	MS.gene02252:CDS
GAGAATCTAGCGTGGAGCTC+CGG	0.500119	2.1:-72942240	MS.gene02252:CDS
ATCTGTGCCACTCATGTCGC+CGG	0.501762	2.1:+72942221	None:intergenic
CCTGACGTCTTGCTTCGTTG+TGG	0.534137	2.1:+72942379	None:intergenic
CGATTGATCCACTCCGGTGG+TGG	0.538844	2.1:-72942276	MS.gene02252:CDS
CACAGATGCAGTGAGGTTGT+GGG	0.544377	2.1:-72942205	MS.gene02252:CDS
CGGTGGTGGTGGCCGTTACG+GGG	0.566376	2.1:-72942262	MS.gene02252:CDS
CCACAACGAAGCAAGACGTC+AGG	0.568340	2.1:-72942379	MS.gene02252:CDS
TGGGCGTTCACATAGTCTGT+TGG	0.581596	2.1:+72942399	None:intergenic
TGGAGCTCCGGCGACATGAG+TGG	0.603470	2.1:-72942228	MS.gene02252:CDS
AGACTATGCTAATCAACGCA+AGG	0.607234	2.1:-72942307	MS.gene02252:CDS
GATCCACCAGGCAACTATGT+TGG	0.607501	2.1:-72942024	MS.gene02252:CDS
AACGGCCACCACCACCGGAG+TGG	0.614459	2.1:+72942268	None:intergenic
TGACCAACATAGTTGCCTGG+TGG	0.614666	2.1:+72942021	None:intergenic
GACTATGCTAATCAACGCAA+GGG	0.626800	2.1:-72942306	MS.gene02252:CDS
GTGTGGCCATTATACTCAGG+TGG	0.627875	2.1:-72942127	MS.gene02252:CDS
GATTGTCGATTGATCCACTC+CGG	0.648727	2.1:-72942282	MS.gene02252:CDS
AGTGTGTGGCCATTATACTC+AGG	0.651003	2.1:-72942130	MS.gene02252:CDS
AACGAAGCAAGACGTCAGGT+TGG	0.655351	2.1:-72942375	MS.gene02252:CDS
ACGCTAGATTCTCCCCGTAA+CGG	0.657514	2.1:+72942250	None:intergenic
ACTTGCAATTACGATCCACC+AGG	0.660677	2.1:-72942036	MS.gene02252:CDS
GTTACGGGGAGAATCTAGCG+TGG	0.716019	2.1:-72942248	MS.gene02252:CDS
TGTCGATTGATCCACTCCGG+TGG	0.719544	2.1:-72942279	MS.gene02252:CDS
TGTGCTGCTAATAAAGTGTG+TGG	0.730769	2.1:-72942144	MS.gene02252:CDS
TGAGTGGCACAGATGCAGTG+AGG	0.786885	2.1:-72942212	MS.gene02252:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTACTATTATAGTTATAAT+CGG	+	chr2.1:72942315-72942334	None:intergenic	15.0%
!!	ATTATTATCACATTTCACTT+TGG	+	chr2.1:72942411-72942430	None:intergenic	20.0%
!	GTGAAATGTGATAATAATAG+AGG	-	chr2.1:72942413-72942432	MS.gene02252:CDS	25.0%
!!	TTAGGCTTATTTCTCATTAT+TGG	-	chr2.1:72942035-72942054	MS.gene02252:CDS	25.0%
!	TTTTCTCTAATGTGTGTCTT+AGG	-	chr2.1:72942017-72942036	MS.gene02252:CDS	30.0%
!!	ATAGTCTGTTGGTGAGTTTT+GGG	+	chr2.1:72942075-72942094	None:intergenic	35.0%
!!	TGGTGTTGCAAATGTTGTTT+GGG	-	chr2.1:72942127-72942146	MS.gene02252:CDS	35.0%
!!	TTGGTGTTGCAAATGTTGTT+TGG	-	chr2.1:72942126-72942145	MS.gene02252:CDS	35.0%
	AGACTATGCTAATCAACGCA+AGG	-	chr2.1:72942175-72942194	MS.gene02252:CDS	40.0%
	GACTATGCTAATCAACGCAA+GGG	-	chr2.1:72942176-72942195	MS.gene02252:CDS	40.0%
	TCCAAACCACCTGAGTATAA+TGG	+	chr2.1:72942364-72942383	None:intergenic	40.0%
	TGGAGAAACACAAAGCGTAT+TGG	-	chr2.1:72942380-72942399	MS.gene02252:CDS	40.0%
!	TGTGCTGCTAATAAAGTGTG+TGG	-	chr2.1:72942338-72942357	MS.gene02252:CDS	40.0%
!	TTTTGACCAACATAGTTGCC+TGG	+	chr2.1:72942467-72942486	None:intergenic	40.0%
!!	CATAGTCTGTTGGTGAGTTT+TGG	+	chr2.1:72942076-72942095	None:intergenic	40.0%
	ACTTGCAATTACGATCCACC+AGG	-	chr2.1:72942446-72942465	MS.gene02252:CDS	45.0%
	AGTGTGTGGCCATTATACTC+AGG	-	chr2.1:72942352-72942371	MS.gene02252:CDS	45.0%
	GCCATTATACTCAGGTGGTT+TGG	-	chr2.1:72942360-72942379	MS.gene02252:CDS	45.0%
!	GATTGTCGATTGATCCACTC+CGG	-	chr2.1:72942200-72942219	MS.gene02252:CDS	45.0%
	AACGAAGCAAGACGTCAGGT+TGG	-	chr2.1:72942107-72942126	MS.gene02252:CDS	50.0%
	ACGCTAGATTCTCCCCGTAA+CGG	+	chr2.1:72942235-72942254	None:intergenic	50.0%
	CACAGATGCAGTGAGGTTGT+GGG	-	chr2.1:72942277-72942296	MS.gene02252:CDS	50.0%
	CTGACGTCTTGCTTCGTTGT+GGG	+	chr2.1:72942105-72942124	None:intergenic	50.0%
	GATCCACCAGGCAACTATGT+TGG	-	chr2.1:72942458-72942477	MS.gene02252:CDS	50.0%
	GTGTGGCCATTATACTCAGG+TGG	-	chr2.1:72942355-72942374	MS.gene02252:CDS	50.0%
	TGACCAACATAGTTGCCTGG+TGG	+	chr2.1:72942464-72942483	None:intergenic	50.0%
	TGGGCGTTCACATAGTCTGT+TGG	+	chr2.1:72942086-72942105	None:intergenic	50.0%
	CCACAACGAAGCAAGACGTC+AGG	-	chr2.1:72942103-72942122	MS.gene02252:CDS	55.0%
	CCTGACGTCTTGCTTCGTTG+TGG	+	chr2.1:72942106-72942125	None:intergenic	55.0%
	GAGAATCTAGCGTGGAGCTC+CGG	-	chr2.1:72942242-72942261	MS.gene02252:CDS	55.0%
	GCACAGATGCAGTGAGGTTG+TGG	-	chr2.1:72942276-72942295	MS.gene02252:CDS	55.0%
	GTTACGGGGAGAATCTAGCG+TGG	-	chr2.1:72942234-72942253	MS.gene02252:CDS	55.0%
	TGAGTGGCACAGATGCAGTG+AGG	-	chr2.1:72942270-72942289	MS.gene02252:CDS	55.0%
!	ATCTGTGCCACTCATGTCGC+CGG	+	chr2.1:72942264-72942283	None:intergenic	55.0%
!	TGTCGATTGATCCACTCCGG+TGG	-	chr2.1:72942203-72942222	MS.gene02252:CDS	55.0%
	CGATTGATCCACTCCGGTGG+TGG	-	chr2.1:72942206-72942225	MS.gene02252:CDS	60.0%
!	TTGATCCACTCCGGTGGTGG+TGG	-	chr2.1:72942209-72942228	MS.gene02252:CDS	60.0%
	TGGAGCTCCGGCGACATGAG+TGG	-	chr2.1:72942254-72942273	MS.gene02252:CDS	65.0%
!!	TCCGGTGGTGGTGGCCGTTA+CGG	-	chr2.1:72942218-72942237	MS.gene02252:CDS	65.0%
	AACGGCCACCACCACCGGAG+TGG	+	chr2.1:72942217-72942236	None:intergenic	70.0%
	CCCGTAACGGCCACCACCAC+CGG	+	chr2.1:72942222-72942241	None:intergenic	70.0%
!!	CCGGTGGTGGTGGCCGTTAC+GGG	-	chr2.1:72942219-72942238	MS.gene02252:CDS	70.0%
!!	CGGTGGTGGTGGCCGTTACG+GGG	-	chr2.1:72942220-72942239	MS.gene02252:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	72942008	72942496	72942008	ID=MS.gene02252
chr2.1	mRNA	72942008	72942496	72942008	ID=MS.gene02252.t1;Parent=MS.gene02252
chr2.1	exon	72942008	72942496	72942008	ID=MS.gene02252.t1.exon1;Parent=MS.gene02252.t1
chr2.1	CDS	72942008	72942496	72942008	ID=cds.MS.gene02252.t1;Parent=MS.gene02252.t1

Gene Sequence

>MS.gene02252

ATGAGTTCATTTTCTCTAATGTGTGTCTTAGGCTTATTTCTCATTATTGGTCACGTTGCACATGCCCAAAACTCACCAACAGACTATGTGAACGCCCACAACGAAGCAAGACGTCAGGTTGGTGTTGCAAATGTTGTTTGGGACAATAACGTTGCTGCTTTTGCACAAGACTATGCTAATCAACGCAAGGGTGATTGTCGATTGATCCACTCCGGTGGTGGTGGCCGTTACGGGGAGAATCTAGCGTGGAGCTCCGGCGACATGAGTGGCACAGATGCAGTGAGGTTGTGGGTGAATGAGAGAGCCGATTATAACTATAATAGTAACACATGTGCTGCTAATAAAGTGTGTGGCCATTATACTCAGGTGGTTTGGAGAAACACAAAGCGTATTGGATGTGCCAAAGTGAAATGTGATAATAATAGAGGAACATTCATTACTTGCAATTACGATCCACCAGGCAACTATGTTGGTCAAAAGCCATACTAA

Protein sequence

>MS.gene02252.t1

MSSFSLMCVLGLFLIIGHVAHAQNSPTDYVNAHNEARRQVGVANVVWDNNVAAFAQDYANQRKGDCRLIHSGGGGRYGENLAWSSGDMSGTDAVRLWVNERADYNYNSNTCAANKVCGHYTQVVWRNTKRIGCAKVKCDNNRGTFITCNYDPPGNYVGQKPY