AlfalfaGEDB | MS.gene002294

M. sativa cultivar XinJiangDaYe / MS.gene002294

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002294.t1	RHN71757.1	86.1	151	21	0	1	151	280	430	7.30E-76	293.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002294.t1	Q9ZNS4	68.1	135	38	3	16	150	26	155	2.2e-49	196.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002294.t1	G7IFD1	86.1	151	21	0	1	151	7	157	5.3e-76	293.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene02255	MS.gene002294	PPI
MS.gene002294	MS.gene002293	PPI
MS.gene002294	MS.gene01270	PPI
MS.gene00249	MS.gene002294	PPI
MS.gene01272	MS.gene002294	PPI
MS.gene002294	MS.gene002297	PPI
MS.gene02256	MS.gene002294	PPI
MS.gene01688	MS.gene002294	PPI
MS.gene01356	MS.gene002294	PPI
MS.gene02254	MS.gene002294	PPI
MS.gene01273	MS.gene002294	PPI
MS.gene018353	MS.gene002294	PPI
MS.gene002292	MS.gene002294	PPI
MS.gene002299	MS.gene002294	PPI
MS.gene002294	MS.gene01274	PPI
MS.gene01686	MS.gene002294	PPI
MS.gene002295	MS.gene002294	PPI
MS.gene57900	MS.gene002294	PPI
MS.gene002294	MS.gene002296	PPI
MS.gene02252	MS.gene002294	PPI
MS.gene035685	MS.gene002294	PPI
MS.gene01685	MS.gene002294	PPI
MS.gene02249	MS.gene002294	PPI
MS.gene01275	MS.gene002294	PPI
MS.gene012420	MS.gene002294	PPI
MS.gene002294	MS.gene02258	PPI
MS.gene01687	MS.gene002294	PPI
MS.gene01277	MS.gene002294	PPI
MS.gene035687	MS.gene002294	PPI
MS.gene01690	MS.gene002294	PPI
MS.gene01684	MS.gene002294	PPI
MS.gene028545	MS.gene002294	PPI
MS.gene002294	MS.gene038936	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002294.t1	MTR_2g010600	86.093	151	21	0	1	151	7	157	2.11e-99	283
MS.gene002294.t1	MTR_4g050762	86.014	143	20	0	9	151	15	157	3.33e-93	268
MS.gene002294.t1	MTR_2g012370	86.014	143	20	0	9	151	15	157	3.33e-93	268
MS.gene002294.t1	MTR_2g010590	82.119	151	24	2	1	151	7	154	8.00e-88	253
MS.gene002294.t1	MTR_2g010630	71.812	149	35	4	8	151	14	160	4.79e-73	216
MS.gene002294.t1	MTR_2g010650	68.153	157	40	6	1	151	8	160	2.01e-71	212
MS.gene002294.t1	MTR_2g010610	69.718	142	20	2	9	150	15	133	1.42e-67	201
MS.gene002294.t1	MTR_2g435490	56.849	146	61	1	6	151	16	159	7.91e-63	191
MS.gene002294.t1	MTR_2g010700	59.873	157	53	5	1	149	7	161	1.68e-61	187
MS.gene002294.t1	MTR_2g010640	59.477	153	55	5	1	149	7	156	4.46e-59	181
MS.gene002294.t1	MTR_2g010670	58.553	152	55	5	3	149	9	157	1.88e-53	166
MS.gene002294.t1	MTR_2g010690	54.605	152	61	5	3	149	9	157	1.72e-50	159
MS.gene002294.t1	MTR_2g010580	48.026	152	70	4	3	149	10	157	2.42e-49	156
MS.gene002294.t1	MTR_4g092540	47.368	152	75	4	1	151	7	154	6.13e-46	147
MS.gene002294.t1	MTR_4g128750	51.163	129	60	2	23	150	41	167	1.40e-41	137
MS.gene002294.t1	MTR_4g128770	45.113	133	69	3	22	151	70	201	1.25e-37	127
MS.gene002294.t1	MTR_1g067290	38.346	133	77	4	22	151	73	203	2.07e-30	109
MS.gene002294.t1	MTR_4g092515	52.941	102	41	3	1	97	7	106	9.62e-26	95.5
MS.gene002294.t1	MTR_8g078770	37.594	133	79	4	22	151	36	167	9.85e-24	91.3
MS.gene002294.t1	MTR_5g018770	33.824	136	82	5	22	151	47	180	9.13e-22	86.7
MS.gene002294.t1	MTR_5g018755	32.143	84	54	2	68	148	10	93	4.84e-12	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002294.t1	AT4G33720	60.265	151	57	2	1	151	11	158	6.93e-64	193
MS.gene002294.t1	AT5G26130	59.333	150	60	1	1	150	12	160	4.19e-62	188
MS.gene002294.t1	AT2G14580	68.148	135	38	3	16	150	26	155	6.35e-61	185
MS.gene002294.t1	AT2G14610	63.043	138	46	3	14	151	24	156	8.40e-58	177
MS.gene002294.t1	AT4G33710	62.687	134	49	1	16	149	27	159	1.39e-56	174
MS.gene002294.t1	AT3G19690	53.595	153	64	4	1	151	9	156	1.43e-53	167
MS.gene002294.t1	AT1G50060	55.479	146	59	4	7	151	16	156	9.27e-47	149
MS.gene002294.t1	AT4G25790	52.672	131	59	2	22	151	77	205	9.77e-47	151
MS.gene002294.t1	AT2G19990	49.242	132	65	1	20	151	42	171	2.13e-45	146
MS.gene002294.t1	AT5G57625	51.538	130	60	2	23	151	75	202	4.40e-45	147
MS.gene002294.t1	AT3G09590	47.222	144	71	3	9	150	40	180	1.32e-42	140
MS.gene002294.t1	AT1G50050	56.000	150	57	4	7	149	8	155	3.36e-41	135
MS.gene002294.t1	AT4G30320	44.521	146	77	3	7	151	14	156	4.47e-41	135
MS.gene002294.t1	AT4G25780	48.872	133	64	3	22	151	54	185	6.95e-41	135
MS.gene002294.t1	AT4G33730	51.128	133	60	4	20	151	39	167	8.56e-41	134
MS.gene002294.t1	AT1G50050	56.164	146	55	4	7	145	8	151	2.23e-39	132
MS.gene002294.t1	AT5G02730	46.565	131	66	2	22	150	59	187	8.55e-38	128
MS.gene002294.t1	AT4G07820	46.970	132	66	3	15	146	24	151	6.43e-37	124
MS.gene002294.t1	AT1G01310	45.385	130	67	3	22	150	87	213	6.43e-36	124
MS.gene002294.t1	AT4G31470	45.802	131	67	3	22	151	53	180	1.74e-35	121
MS.gene002294.t1	AT5G66590	43.704	135	67	3	23	151	49	180	2.52e-33	116
MS.gene002294.t1	AT2G19970	39.706	136	69	6	20	146	35	166	3.46e-25	95.1
MS.gene002294.t1	AT2G19980	38.168	131	66	5	20	146	35	154	1.78e-22	87.8

Find 40 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATAGTCTGTTGGTGAGTTT+TGG	0.180317	2.3:+72837443	None:intergenic
TTAGGCTTATTTCTCATTAT+TGG	0.192429	2.3:-72837481	MS.gene002294:CDS
CCGGTGGTGGTGGCCGTTAC+GGG	0.193193	2.3:-72837297	MS.gene002294:CDS
TCCGGTGGTGGTGGCCGTTA+CGG	0.303334	2.3:-72837298	MS.gene002294:CDS
TTGGTGTTGCAAATGTTGTT+TGG	0.348141	2.3:-72837390	MS.gene002294:CDS
GCCATTATACTCAGGTGGTT+TGG	0.358578	2.3:-72837156	MS.gene002294:CDS
GTTACTATTATAATTATAAT+CGG	0.388230	2.3:+72837204	None:intergenic
TCCAAACCACCTGAGTATAA+TGG	0.398812	2.3:+72837155	None:intergenic
TGGTGTTGCAAATGTTGTTT+GGG	0.411343	2.3:-72837389	MS.gene002294:CDS
CTGACGTCTTGCTTCGTTGT+GGG	0.413555	2.3:+72837414	None:intergenic
CCCGTAACGGCCACCACCAC+CGG	0.429882	2.3:+72837297	None:intergenic
TTGATCCACTCCGGTGGTGG+TGG	0.451842	2.3:-72837307	MS.gene002294:CDS
TGGAGAAACACAAAGCGTAT+TGG	0.455942	2.3:-72837136	MS.gene002294:CDS
GCACAGATGCAGTGAGGTTG+TGG	0.462999	2.3:-72837240	MS.gene002294:CDS
ATTATTATCACATTTCACTT+TGG	0.463790	2.3:+72837108	None:intergenic
GTGAAATGTGATAATAATAG+AGG	0.492513	2.3:-72837103	MS.gene002294:CDS
GAGAATCTAGCGTGGAGCTC+CGG	0.500119	2.3:-72837274	MS.gene002294:CDS
ATCTGTGCCACTCATGTCGC+CGG	0.501762	2.3:+72837255	None:intergenic
CCTGACGTCTTGCTTCGTTG+TGG	0.534137	2.3:+72837413	None:intergenic
CACAGATGCAGTGAGGTTGT+GGG	0.544377	2.3:-72837239	MS.gene002294:CDS
CAATTGATCCACTCCGGTGG+TGG	0.548354	2.3:-72837310	MS.gene002294:CDS
CGGTGGTGGTGGCCGTTACG+GGG	0.566376	2.3:-72837296	MS.gene002294:CDS
CCACAACGAAGCAAGACGTC+AGG	0.568340	2.3:-72837413	MS.gene002294:CDS
TGGGCGTTCACATAGTCTGT+TGG	0.581596	2.3:+72837433	None:intergenic
AAACTATGCTAATCAACGCA+AGG	0.596763	2.3:-72837341	MS.gene002294:CDS
TGGAGCTCCGGCGACATGAG+TGG	0.603470	2.3:-72837262	MS.gene002294:CDS
GATCCACCAGGCAACTATGT+TGG	0.607501	2.3:-72837058	None:intergenic
AACGGCCACCACCACCGGAG+TGG	0.614459	2.3:+72837302	None:intergenic
TGACCAACATAGTTGCCTGG+TGG	0.614666	2.3:+72837055	None:intergenic
AACTATGCTAATCAACGCAA+GGG	0.616329	2.3:-72837340	MS.gene002294:CDS
GTGTGGCCATTATACTCAGG+TGG	0.627875	2.3:-72837161	MS.gene002294:CDS
GATTGTCAATTGATCCACTC+CGG	0.634101	2.3:-72837316	MS.gene002294:CDS
AGTGTGTGGCCATTATACTC+AGG	0.651003	2.3:-72837164	MS.gene002294:CDS
AACGAAGCAAGACGTCAGGT+TGG	0.655351	2.3:-72837409	MS.gene002294:CDS
ACGCTAGATTCTCCCCGTAA+CGG	0.657514	2.3:+72837284	None:intergenic
ACTTGCAATTACGATCCACC+AGG	0.660677	2.3:-72837070	MS.gene002294:CDS
TGTCAATTGATCCACTCCGG+TGG	0.707723	2.3:-72837313	MS.gene002294:CDS
GTTACGGGGAGAATCTAGCG+TGG	0.716019	2.3:-72837282	MS.gene002294:CDS
TGTGCTGCTAATAAAGTGTG+TGG	0.730769	2.3:-72837178	MS.gene002294:CDS
TGAGTGGCACAGATGCAGTG+AGG	0.786885	2.3:-72837246	MS.gene002294:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTACTATTATAATTATAAT+CGG	+	chr2.3:72837348-72837367	None:intergenic	10.0%
!!	ATTATTATCACATTTCACTT+TGG	+	chr2.3:72837444-72837463	None:intergenic	20.0%
!	GTGAAATGTGATAATAATAG+AGG	-	chr2.3:72837446-72837465	MS.gene002294:CDS	25.0%
!!	TTAGGCTTATTTCTCATTAT+TGG	-	chr2.3:72837068-72837087	MS.gene002294:CDS	25.0%
	AAACTATGCTAATCAACGCA+AGG	-	chr2.3:72837208-72837227	MS.gene002294:CDS	35.0%
	AACTATGCTAATCAACGCAA+GGG	-	chr2.3:72837209-72837228	MS.gene002294:CDS	35.0%
!!	ATAGTCTGTTGGTGAGTTTT+GGG	+	chr2.3:72837108-72837127	None:intergenic	35.0%
!!	TGGTGTTGCAAATGTTGTTT+GGG	-	chr2.3:72837160-72837179	MS.gene002294:CDS	35.0%
!!	TTGGTGTTGCAAATGTTGTT+TGG	-	chr2.3:72837159-72837178	MS.gene002294:CDS	35.0%
	GATTGTCAATTGATCCACTC+CGG	-	chr2.3:72837233-72837252	MS.gene002294:CDS	40.0%
	TCCAAACCACCTGAGTATAA+TGG	+	chr2.3:72837397-72837416	None:intergenic	40.0%
	TGGAGAAACACAAAGCGTAT+TGG	-	chr2.3:72837413-72837432	MS.gene002294:CDS	40.0%
!	TGTGCTGCTAATAAAGTGTG+TGG	-	chr2.3:72837371-72837390	MS.gene002294:CDS	40.0%
!!	CATAGTCTGTTGGTGAGTTT+TGG	+	chr2.3:72837109-72837128	None:intergenic	40.0%
	ACTTGCAATTACGATCCACC+AGG	-	chr2.3:72837479-72837498	MS.gene002294:CDS	45.0%
	AGTGTGTGGCCATTATACTC+AGG	-	chr2.3:72837385-72837404	MS.gene002294:CDS	45.0%
	GCCATTATACTCAGGTGGTT+TGG	-	chr2.3:72837393-72837412	MS.gene002294:CDS	45.0%
	AACGAAGCAAGACGTCAGGT+TGG	-	chr2.3:72837140-72837159	MS.gene002294:CDS	50.0%
	ACGCTAGATTCTCCCCGTAA+CGG	+	chr2.3:72837268-72837287	None:intergenic	50.0%
	CACAGATGCAGTGAGGTTGT+GGG	-	chr2.3:72837310-72837329	MS.gene002294:CDS	50.0%
	CTGACGTCTTGCTTCGTTGT+GGG	+	chr2.3:72837138-72837157	None:intergenic	50.0%
	GTGTGGCCATTATACTCAGG+TGG	-	chr2.3:72837388-72837407	MS.gene002294:CDS	50.0%
	TGGGCGTTCACATAGTCTGT+TGG	+	chr2.3:72837119-72837138	None:intergenic	50.0%
	TGTCAATTGATCCACTCCGG+TGG	-	chr2.3:72837236-72837255	MS.gene002294:CDS	50.0%
	CAATTGATCCACTCCGGTGG+TGG	-	chr2.3:72837239-72837258	MS.gene002294:CDS	55.0%
	CCACAACGAAGCAAGACGTC+AGG	-	chr2.3:72837136-72837155	MS.gene002294:CDS	55.0%
	CCTGACGTCTTGCTTCGTTG+TGG	+	chr2.3:72837139-72837158	None:intergenic	55.0%
	GAGAATCTAGCGTGGAGCTC+CGG	-	chr2.3:72837275-72837294	MS.gene002294:CDS	55.0%
	GCACAGATGCAGTGAGGTTG+TGG	-	chr2.3:72837309-72837328	MS.gene002294:CDS	55.0%
	GTTACGGGGAGAATCTAGCG+TGG	-	chr2.3:72837267-72837286	MS.gene002294:CDS	55.0%
	TGAGTGGCACAGATGCAGTG+AGG	-	chr2.3:72837303-72837322	MS.gene002294:CDS	55.0%
!	ATCTGTGCCACTCATGTCGC+CGG	+	chr2.3:72837297-72837316	None:intergenic	55.0%
!	TTGATCCACTCCGGTGGTGG+TGG	-	chr2.3:72837242-72837261	MS.gene002294:CDS	60.0%
	TGGAGCTCCGGCGACATGAG+TGG	-	chr2.3:72837287-72837306	MS.gene002294:CDS	65.0%
!!	TCCGGTGGTGGTGGCCGTTA+CGG	-	chr2.3:72837251-72837270	MS.gene002294:CDS	65.0%
	AACGGCCACCACCACCGGAG+TGG	+	chr2.3:72837250-72837269	None:intergenic	70.0%
	CCCGTAACGGCCACCACCAC+CGG	+	chr2.3:72837255-72837274	None:intergenic	70.0%
!!	CCGGTGGTGGTGGCCGTTAC+GGG	-	chr2.3:72837252-72837271	MS.gene002294:CDS	70.0%
!!	CGGTGGTGGTGGCCGTTACG+GGG	-	chr2.3:72837253-72837272	MS.gene002294:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	72837059	72837512	72837059	ID=MS.gene002294
chr2.3	mRNA	72837059	72837512	72837059	ID=MS.gene002294.t1;Parent=MS.gene002294
chr2.3	exon	72837059	72837512	72837059	ID=MS.gene002294.t1.exon1;Parent=MS.gene002294.t1
chr2.3	CDS	72837059	72837512	72837059	ID=cds.MS.gene002294.t1;Parent=MS.gene002294.t1

Gene Sequence

>MS.gene002294

ATGTGTGTCTTAGGCTTATTTCTCATTATTGGTCGCGTTGCACATGCCCAAAACTCACCAACAGACTATGTGAACGCCCACAACGAAGCAAGACGTCAGGTTGGTGTTGCAAATGTTGTTTGGGACAATACTGTTGCTGCTTTTGCACAAAACTATGCTAATCAACGCAAGGGTGATTGTCAATTGATCCACTCCGGTGGTGGTGGCCGTTACGGGGAGAATCTAGCGTGGAGCTCCGGCGACATGAGTGGCACAGATGCAGTGAGGTTGTGGGTGAATGAGAGAGCCGATTATAATTATAATAGTAACACATGTGCTGCTAATAAAGTGTGTGGCCATTATACTCAGGTGGTTTGGAGAAACACAAAGCGTATTGGATGTGCCAAAGTGAAATGTGATAATAATAGAGGAACATTCATTACTTGCAATTACGATCCACCAGGCAACTATGTTG

Protein sequence

>MS.gene002294.t1

MCVLGLFLIIGRVAHAQNSPTDYVNAHNEARRQVGVANVVWDNTVAAFAQNYANQRKGDCQLIHSGGGGRYGENLAWSSGDMSGTDAVRLWVNERADYNYNSNTCAANKVCGHYTQVVWRNTKRIGCAKVKCDNNRGTFITCNYDPPGNYV