AlfalfaGEDB | MS.gene02249

M. sativa cultivar XinJiangDaYe / MS.gene02249

KEGG: K13449
GO: GO:0005575 GO:0005576 GO:0110165
PFAM: PF00188.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02249.t1	AES63607.2	94.9	157	8	0	6	162	8	164	1.50E-87	332

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02249.t1	P11670	58.1	155	63	1	8	162	7	159	5.2e-52	205.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02249.t1	G7IFC9	94.9	157	8	0	6	162	8	164	1.1e-87	332.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene02249	MS.gene02252	PPI
MS.gene01687	MS.gene02249	PPI
MS.gene02249	MS.gene01274	PPI
MS.gene02249	MS.gene01356	PPI
MS.gene02249	MS.gene002296	PPI
MS.gene02249	MS.gene002297	PPI
MS.gene02249	MS.gene01277	PPI
MS.gene02249	MS.gene02256	PPI
MS.gene02249	MS.gene01690	PPI
MS.gene02249	MS.gene01686	PPI
MS.gene02249	MS.gene002295	PPI
MS.gene02249	MS.gene002294	PPI
MS.gene02249	MS.gene01275	PPI
MS.gene02249	MS.gene02258	PPI
MS.gene02255	MS.gene02249	PPI
MS.gene02249	MS.gene01685	PPI
MS.gene02254	MS.gene02249	PPI
MS.gene01273	MS.gene02249	PPI
MS.gene002299	MS.gene02249	PPI
MS.gene01272	MS.gene02249	PPI
MS.gene01688	MS.gene02249	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02249.t1	MTR_2g010580	94.904	157	8	0	6	162	8	164	7.64e-113	317
MS.gene02249.t1	MTR_4g092540	56.688	157	62	3	7	162	8	159	1.27e-62	190
MS.gene02249.t1	MTR_2g010590	50.000	156	72	2	8	162	9	159	9.04e-57	175
MS.gene02249.t1	MTR_2g435490	51.220	164	78	2	1	162	1	164	2.42e-56	175
MS.gene02249.t1	MTR_2g010650	50.000	162	75	3	6	162	5	165	1.90e-54	169
MS.gene02249.t1	MTR_2g010630	53.061	147	61	4	23	162	20	165	2.68e-51	161
MS.gene02249.t1	MTR_2g010600	49.333	150	72	3	17	162	13	162	5.91e-51	160
MS.gene02249.t1	MTR_4g050762	50.694	144	67	3	23	162	19	162	4.48e-50	159
MS.gene02249.t1	MTR_2g012370	50.694	144	67	3	23	162	19	162	4.48e-50	159
MS.gene02249.t1	MTR_2g010690	43.827	162	80	4	7	162	8	164	1.69e-45	147
MS.gene02249.t1	MTR_2g010670	43.210	162	83	4	9	162	4	164	2.60e-44	144
MS.gene02249.t1	MTR_2g010640	43.750	160	80	4	8	162	9	163	1.90e-43	141
MS.gene02249.t1	MTR_2g010700	44.000	150	73	5	23	162	20	168	4.43e-41	135
MS.gene02249.t1	MTR_2g010610	46.479	142	52	4	23	162	19	138	7.62e-40	131
MS.gene02249.t1	MTR_4g128750	47.518	141	71	3	25	162	33	173	4.73e-37	125
MS.gene02249.t1	MTR_4g128770	44.526	137	70	3	32	162	70	206	7.61e-33	115
MS.gene02249.t1	MTR_1g067290	39.259	135	77	3	32	161	73	207	1.46e-27	102
MS.gene02249.t1	MTR_8g078770	37.349	166	88	7	8	162	12	172	3.42e-27	100
MS.gene02249.t1	MTR_5g018770	33.708	178	91	7	11	162	9	185	2.63e-24	93.6
MS.gene02249.t1	MTR_4g092515	49.367	79	39	1	8	86	9	86	5.96e-22	85.9
MS.gene02249.t1	MTR_5g018755	37.079	89	50	4	80	162	15	103	9.91e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02249.t1	AT4G33720	54.037	161	70	2	3	162	6	163	3.46e-59	181
MS.gene02249.t1	AT2G14610	53.289	152	70	1	11	162	11	161	8.66e-57	175
MS.gene02249.t1	AT2G14580	51.299	154	72	2	11	162	9	161	1.16e-56	175
MS.gene02249.t1	AT2G19990	49.697	165	74	3	7	162	12	176	3.09e-56	174
MS.gene02249.t1	AT3G19690	51.049	143	65	2	24	162	20	161	9.29e-51	160
MS.gene02249.t1	AT1G50060	49.390	164	75	3	1	162	4	161	1.17e-50	160
MS.gene02249.t1	AT5G26130	48.485	165	80	3	1	162	4	166	2.23e-50	159
MS.gene02249.t1	AT4G33730	49.351	154	69	2	18	162	19	172	3.47e-47	151
MS.gene02249.t1	AT4G33710	51.064	141	66	2	25	162	26	166	1.89e-46	149
MS.gene02249.t1	AT4G25790	48.148	135	67	3	31	162	76	210	7.66e-40	134
MS.gene02249.t1	AT4G30320	42.073	164	90	3	1	162	1	161	4.74e-39	130
MS.gene02249.t1	AT4G07820	46.763	139	71	3	25	162	24	160	4.97e-39	130
MS.gene02249.t1	AT1G50050	44.848	165	82	4	1	162	4	162	1.19e-38	129
MS.gene02249.t1	AT1G01310	48.872	133	66	2	32	162	87	219	1.31e-38	132
MS.gene02249.t1	AT5G57625	48.120	133	66	3	33	162	75	207	4.99e-38	129
MS.gene02249.t1	AT4G31470	44.776	134	72	2	31	162	52	185	2.81e-36	124
MS.gene02249.t1	AT2G19970	43.750	144	69	5	30	162	35	177	2.98e-36	124
MS.gene02249.t1	AT3G09590	40.411	146	83	3	21	162	41	186	4.64e-35	121
MS.gene02249.t1	AT1G50050	42.331	163	85	4	1	160	4	160	1.08e-34	121
MS.gene02249.t1	AT5G02730	42.466	146	80	3	21	162	48	193	2.71e-34	119
MS.gene02249.t1	AT4G25780	41.772	158	82	5	11	162	37	190	1.45e-33	117
MS.gene02249.t1	AT5G66590	42.553	141	74	5	29	162	45	185	6.63e-32	113
MS.gene02249.t1	AT2G19980	40.120	167	74	7	13	162	8	165	1.37e-31	111

Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCATTATACTCAAATTATT+TGG	0.165449	2.1:-72977154	MS.gene02249:CDS
TGGTGTTGGTCCACTTTATT+GGG	0.256190	2.1:-72977378	MS.gene02249:CDS
TTGGTGTTGGTCCACTTTAT+TGG	0.259570	2.1:-72977379	MS.gene02249:CDS
CGTTCTCCTTCAACATTTCC+AGG	0.274248	2.1:+72977053	None:intergenic
AACCCAACCATTCTTACATT+TGG	0.275936	2.1:+72977100	None:intergenic
TTAGAGTGATAATCATAATT+AGG	0.290359	2.1:+72977200	None:intergenic
GTACGGATTCTGTGAAGTTT+TGG	0.310490	2.1:-72977235	MS.gene02249:CDS
TTGTGAGCTTGAGCACTCTA+TGG	0.317138	2.1:-72977303	MS.gene02249:CDS
GAGCATAGGCTTCAAGGTTA+TGG	0.354779	2.1:+72977354	None:intergenic
GGTTGAGCAAATGAAGAAAA+TGG	0.360878	2.1:+72977481	None:intergenic
GGGAAATATTAATGCAACTA+AGG	0.364039	2.1:+72977459	None:intergenic
GAGCACTCTATGGGTCCTTA+TGG	0.400158	2.1:-72977293	MS.gene02249:CDS
TGGCGTGATTCAGTTCATCT+TGG	0.443876	2.1:-72977134	MS.gene02249:CDS
GAGAACCTTGCTGAGGGTTA+TGG	0.444830	2.1:-72977269	MS.gene02249:CDS
TATATAAAAGAAAATCAATA+TGG	0.450206	2.1:+72977029	None:intergenic
AGTCCAAATGTAAGAATGGT+TGG	0.453283	2.1:-72977103	MS.gene02249:CDS
GTCCAAATGTAAGAATGGTT+GGG	0.466932	2.1:-72977102	MS.gene02249:CDS
TCACCACCTGGAAATGTTGA+AGG	0.498349	2.1:-72977059	MS.gene02249:CDS
GTACCTCAAGGTAATCTTGA+GGG	0.504079	2.1:+72977423	None:intergenic
GGAAATATTAATGCAACTAA+GGG	0.514988	2.1:+72977460	None:intergenic
TCAGCAAGGTTCTCACCATA+AGG	0.519070	2.1:+72977278	None:intergenic
AGGTTATGGTCCCAATAAAG+TGG	0.549917	2.1:+72977368	None:intergenic
TGTGAGCTTGAGCACTCTAT+GGG	0.560190	2.1:-72977302	MS.gene02249:CDS
TTATGGTGAGAACCTTGCTG+AGG	0.564497	2.1:-72977276	MS.gene02249:CDS
ATGGCTAAGATCATATTATG+TGG	0.564871	2.1:+72977500	None:intergenic
GCTCGAGATGAGGTTGGTGT+TGG	0.566361	2.1:-72977392	MS.gene02249:CDS
GCTAAGTCCAAATGTAAGAA+TGG	0.566456	2.1:-72977107	MS.gene02249:CDS
ATTTGTAGCTATTCACCACC+TGG	0.588741	2.1:-72977071	MS.gene02249:CDS
GAGGGTTATGGTGAAGTGAA+TGG	0.598130	2.1:-72977257	MS.gene02249:CDS
TTATGGTGAAGTGAATGGTA+CGG	0.605852	2.1:-72977252	MS.gene02249:CDS
GAGCTTGATTATGTACCTCA+AGG	0.611826	2.1:+72977411	None:intergenic
TATGGTGAGAACCTTGCTGA+GGG	0.618070	2.1:-72977275	MS.gene02249:CDS
AATCAAGCTCGAGATGAGGT+TGG	0.619159	2.1:-72977398	MS.gene02249:CDS
TGTACCTCAAGGTAATCTTG+AGG	0.625790	2.1:+72977422	None:intergenic
TACCTCAAGGTAATCTTGAG+GGG	0.626699	2.1:+72977424	None:intergenic
TCTTGTGTTAATGATGAGTG+TGG	0.652987	2.1:-72977176	MS.gene02249:CDS
ACATAATCAAGCTCGAGATG+AGG	0.662626	2.1:-72977402	MS.gene02249:CDS
CTTCACCATAACCCTCAGCA+AGG	0.671746	2.1:+72977264	None:intergenic
TCTCCTTCAACATTTCCAGG+TGG	0.683307	2.1:+72977056	None:intergenic
CTTGTGTTAATGATGAGTGT+GGG	0.697366	2.1:-72977175	MS.gene02249:CDS
CTCCCCTCAAGATTACCTTG+AGG	0.725233	2.1:-72977426	MS.gene02249:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAGAGTGATAATCATAATT+AGG	+	chr2.1:72977355-72977374	None:intergenic	20.0%
!	GGAAATATTAATGCAACTAA+GGG	+	chr2.1:72977095-72977114	None:intergenic	25.0%
!	GGCATTATACTCAAATTATT+TGG	-	chr2.1:72977398-72977417	MS.gene02249:CDS	25.0%
	ATGGCTAAGATCATATTATG+TGG	+	chr2.1:72977055-72977074	None:intergenic	30.0%
	GGGAAATATTAATGCAACTA+AGG	+	chr2.1:72977096-72977115	None:intergenic	30.0%
	AACCCAACCATTCTTACATT+TGG	+	chr2.1:72977455-72977474	None:intergenic	35.0%
	AGTCCAAATGTAAGAATGGT+TGG	-	chr2.1:72977449-72977468	MS.gene02249:CDS	35.0%
	CTTGTGTTAATGATGAGTGT+GGG	-	chr2.1:72977377-72977396	MS.gene02249:CDS	35.0%
	GCTAAGTCCAAATGTAAGAA+TGG	-	chr2.1:72977445-72977464	MS.gene02249:CDS	35.0%
	GGTTGAGCAAATGAAGAAAA+TGG	+	chr2.1:72977074-72977093	None:intergenic	35.0%
	GTCCAAATGTAAGAATGGTT+GGG	-	chr2.1:72977450-72977469	MS.gene02249:CDS	35.0%
	TCTTGTGTTAATGATGAGTG+TGG	-	chr2.1:72977376-72977395	MS.gene02249:CDS	35.0%
	TTATGGTGAAGTGAATGGTA+CGG	-	chr2.1:72977300-72977319	MS.gene02249:CDS	35.0%
!!!	GTTTGCATAGTTTTGAGCAT+AGG	+	chr2.1:72977215-72977234	None:intergenic	35.0%
	ACATAATCAAGCTCGAGATG+AGG	-	chr2.1:72977150-72977169	MS.gene02249:CDS	40.0%
	AGGTTATGGTCCCAATAAAG+TGG	+	chr2.1:72977187-72977206	None:intergenic	40.0%
	ATTTGTAGCTATTCACCACC+TGG	-	chr2.1:72977481-72977500	MS.gene02249:CDS	40.0%
	GAGCTTGATTATGTACCTCA+AGG	+	chr2.1:72977144-72977163	None:intergenic	40.0%
	GTACCTCAAGGTAATCTTGA+GGG	+	chr2.1:72977132-72977151	None:intergenic	40.0%
	TACCTCAAGGTAATCTTGAG+GGG	+	chr2.1:72977131-72977150	None:intergenic	40.0%
	TGTACCTCAAGGTAATCTTG+AGG	+	chr2.1:72977133-72977152	None:intergenic	40.0%
!	GTACGGATTCTGTGAAGTTT+TGG	-	chr2.1:72977317-72977336	MS.gene02249:CDS	40.0%
!!	TGGTGTTGGTCCACTTTATT+GGG	-	chr2.1:72977174-72977193	MS.gene02249:CDS	40.0%
!!	TTGGTGTTGGTCCACTTTAT+TGG	-	chr2.1:72977173-72977192	MS.gene02249:CDS	40.0%
!!!	AGTTTTGAGCATAGGCTTCA+AGG	+	chr2.1:72977207-72977226	None:intergenic	40.0%
	AATCAAGCTCGAGATGAGGT+TGG	-	chr2.1:72977154-72977173	MS.gene02249:CDS	45.0%
	CGTTCTCCTTCAACATTTCC+AGG	+	chr2.1:72977502-72977521	None:intergenic	45.0%
	GAGCATAGGCTTCAAGGTTA+TGG	+	chr2.1:72977201-72977220	None:intergenic	45.0%
	GAGGGTTATGGTGAAGTGAA+TGG	-	chr2.1:72977295-72977314	MS.gene02249:CDS	45.0%
	TATGGTGAGAACCTTGCTGA+GGG	-	chr2.1:72977277-72977296	MS.gene02249:CDS	45.0%
	TCACCACCTGGAAATGTTGA+AGG	-	chr2.1:72977493-72977512	MS.gene02249:CDS	45.0%
	TCAGCAAGGTTCTCACCATA+AGG	+	chr2.1:72977277-72977296	None:intergenic	45.0%
	TCTCCTTCAACATTTCCAGG+TGG	+	chr2.1:72977499-72977518	None:intergenic	45.0%
	TGGCGTGATTCAGTTCATCT+TGG	-	chr2.1:72977418-72977437	MS.gene02249:CDS	45.0%
	TGTGAGCTTGAGCACTCTAT+GGG	-	chr2.1:72977250-72977269	MS.gene02249:CDS	45.0%
	TTATGGTGAGAACCTTGCTG+AGG	-	chr2.1:72977276-72977295	MS.gene02249:CDS	45.0%
	TTGTGAGCTTGAGCACTCTA+TGG	-	chr2.1:72977249-72977268	MS.gene02249:CDS	45.0%
!!	TTGAGGGGAGTTTTGTGCTT+GGG	+	chr2.1:72977116-72977135	None:intergenic	45.0%
	CTCCCCTCAAGATTACCTTG+AGG	-	chr2.1:72977126-72977145	MS.gene02249:CDS	50.0%
	CTTCACCATAACCCTCAGCA+AGG	+	chr2.1:72977291-72977310	None:intergenic	50.0%
	GAGAACCTTGCTGAGGGTTA+TGG	-	chr2.1:72977283-72977302	MS.gene02249:CDS	50.0%
	GAGCACTCTATGGGTCCTTA+TGG	-	chr2.1:72977259-72977278	MS.gene02249:CDS	50.0%
!!	CTTGAGGGGAGTTTTGTGCT+TGG	+	chr2.1:72977117-72977136	None:intergenic	50.0%
!!	GCTCGAGATGAGGTTGGTGT+TGG	-	chr2.1:72977160-72977179	MS.gene02249:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	72977043	72977531	72977043	ID=MS.gene02249
chr2.1	mRNA	72977043	72977531	72977043	ID=MS.gene02249.t1;Parent=MS.gene02249
chr2.1	exon	72977043	72977531	72977043	ID=MS.gene02249.t1.exon1;Parent=MS.gene02249.t1
chr2.1	CDS	72977043	72977531	72977043	ID=cds.MS.gene02249.t1;Parent=MS.gene02249.t1

Gene Sequence

>MS.gene02249

ATGAGGTCTCCACATAATATGATCTTAGCCATTTTCTTCATTTGCTCAACCCTTAGTTGCATTAATATTTCCCAAGCACAAAACTCCCCTCAAGATTACCTTGAGGTACATAATCAAGCTCGAGATGAGGTTGGTGTTGGTCCACTTTATTGGGACCATAACCTTGAAGCCTATGCTCAAAACTATGCAAACAAGAGAATTAAAGATTGTGAGCTTGAGCACTCTATGGGTCCTTATGGTGAGAACCTTGCTGAGGGTTATGGTGAAGTGAATGGTACGGATTCTGTGAAGTTTTGGCTAAGTGAAAAGCCTAATTATGATTATCACTCTAACTCTTGTGTTAATGATGAGTGTGGGCATTATACTCAAATTATTTGGCGTGATTCAGTTCATCTTGGTTGTGCTAAGTCCAAATGTAAGAATGGTTGGGTTTTTGTCATTTGTAGCTATTCACCACCTGGAAATGTTGAAGGAGAACGACCATATTGA

Protein sequence

>MS.gene02249.t1

MRSPHNMILAIFFICSTLSCINISQAQNSPQDYLEVHNQARDEVGVGPLYWDHNLEAYAQNYANKRIKDCELEHSMGPYGENLAEGYGEVNGTDSVKFWLSEKPNYDYHSNSCVNDECGHYTQIIWRDSVHLGCAKSKCKNGWVFVICSYSPPGNVEGERPY