AlfalfaGEDB | MS.gene023274

M. sativa cultivar XinJiangDaYe / MS.gene023274

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023274.t1	ACJ84115.1	90.3	144	10	1	1	140	1	144	1.70E-63	251.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023274.t1	P49215	78.5	144	27	3	1	140	1	144	3.1e-53	209.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023274.t1	B7FH86	90.3	144	10	1	1	140	1	144	1.2e-63	251.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene065715	MS.gene023274	PPI
MS.gene028177	MS.gene023274	PPI
MS.gene039073	MS.gene023274	PPI
MS.gene54136	MS.gene023274	PPI
MS.gene08344	MS.gene023274	PPI
MS.gene34957	MS.gene023274	PPI
MS.gene034834	MS.gene023274	PPI
MS.gene023274	MS.gene040536	PPI
MS.gene023275	MS.gene023274	PPI
MS.gene056917	MS.gene023274	PPI
MS.gene034832	MS.gene023274	PPI
MS.gene08343	MS.gene023274	PPI
MS.gene34958	MS.gene023274	PPI
MS.gene028176	MS.gene023274	PPI
MS.gene039072	MS.gene023274	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023274.t1	MTR_1g058250	92.241	116	9	0	1	116	1	116	2.37e-74	218

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023274.t1	AT5G04800	74.638	138	33	1	1	138	1	136	4.34e-69	205
MS.gene023274.t1	AT5G04800	74.638	138	33	1	1	138	1	136	4.34e-69	205
MS.gene023274.t1	AT5G04800	74.638	138	33	1	1	138	1	136	4.34e-69	205
MS.gene023274.t1	AT5G04800	74.638	138	33	1	1	138	1	136	4.34e-69	205
MS.gene023274.t1	AT2G04390	73.759	141	36	1	1	140	1	141	1.78e-68	203
MS.gene023274.t1	AT3G10610	72.857	140	37	1	1	139	1	140	2.37e-68	203
MS.gene023274.t1	AT2G05220	72.143	140	39	0	1	140	1	140	1.84e-67	201
MS.gene023274.t1	AT2G05220	72.143	140	39	0	1	140	1	140	1.84e-67	201

Find 36 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGGATTTCTTCACGGTTT+TGG	0.259892	4.3:-17455732	None:intergenic
CGTCTTCGTAACAAGATTGC+TGG	0.356034	4.3:+17455848	MS.gene023274:CDS
CTTGGCATGTCGAAGATTCC+TGG	0.368757	4.3:+17456040	MS.gene023274:CDS
TTCTTCAAGGATCTTCTTGT+TGG	0.377402	4.3:-17455804	None:intergenic
CCATATGGGATCTGCTGAAC+AGG	0.380708	4.3:-17456085	None:intergenic
GAAGATTCCTGGTGTTGTTC+AGG	0.386481	4.3:+17456051	MS.gene023274:CDS
TTCCTGAAGTTTCTGCTATT+AGG	0.398820	4.3:+17455969	MS.gene023274:CDS
GTCCTAATAGCAGAAACTTC+AGG	0.409939	4.3:-17455971	None:intergenic
CTAGACATGCTTGCTGCTCT+TGG	0.411310	4.3:+17456022	MS.gene023274:CDS
GCACCAGCACCACGACCATA+TGG	0.428749	4.3:-17456100	None:intergenic
ATCCCATATGGTCGTGGTGC+TGG	0.431174	4.3:+17456097	MS.gene023274:CDS
TATGGTCGTGGTGCTGGTGC+TGG	0.432732	4.3:+17456103	MS.gene023274:CDS
TTCAATGAAATTCCACGAAC+AGG	0.443778	4.3:-17455908	None:intergenic
TTGTTACGAAGACGCTTTGA+AGG	0.464587	4.3:-17455839	None:intergenic
CAAGAGCAGCAAGCATGTCT+AGG	0.475176	4.3:-17456021	None:intergenic
GGATCAAAGCGACTTCTTCA+AGG	0.477079	4.3:-17455817	None:intergenic
ATTCGAAAGGGACCTGTTCG+TGG	0.480777	4.3:+17455896	MS.gene023274:CDS
TGATCATATTGAGGTTGATA+AGG	0.481558	4.3:+17455994	MS.gene023274:CDS
CCTGTTCAGCAGATCCCATA+TGG	0.519231	4.3:+17456085	MS.gene023274:CDS
TTTGGTTCGGACTCGTCCCA+TGG	0.523667	4.3:-17455714	None:intergenic
TTGACGAGAGGATTTCTTCA+CGG	0.524605	4.3:-17455738	None:intergenic
GGAGGAGCGTGATCGTCGTA+TGG	0.525336	4.3:+17455940	MS.gene023274:CDS
TATTAGGACTGATCATATTG+AGG	0.542204	4.3:+17455985	MS.gene023274:CDS
GTGGTGCTGGTGCTGGAAGG+AGG	0.550588	4.3:+17456110	MS.gene023274:CDS
CACCAGCACCACGACCATAT+GGG	0.556729	4.3:-17456099	None:intergenic
CGATCAACCTGAACAACACC+AGG	0.558124	4.3:-17456058	None:intergenic
AAGAGCAGCAAGCATGTCTA+GGG	0.560169	4.3:-17456020	None:intergenic
CTCATGCAGCGTATTCGAAA+GGG	0.564195	4.3:+17455884	MS.gene023274:CDS
GTCGTGGTGCTGGTGCTGGA+AGG	0.566651	4.3:+17456107	MS.gene023274:CDS
TTCATTGAAGCTGCAAGAGG+AGG	0.567846	4.3:+17455922	MS.gene023274:CDS
CAGCAGATCCCATATGGTCG+TGG	0.574554	4.3:+17456091	MS.gene023274:CDS
TCTCATGCAGCGTATTCGAA+AGG	0.588377	4.3:+17455883	MS.gene023274:CDS
TGTTACGAAGACGCTTTGAA+GGG	0.597110	4.3:-17455838	None:intergenic
ACGCTCGATGATTTGACGAG+AGG	0.611995	4.3:-17455750	None:intergenic
TAACCACCCTAAAATGACAC+TGG	0.617615	4.3:+17455772	MS.gene023274:CDS
AATTTCATTGAAGCTGCAAG+AGG	0.634258	4.3:+17455919	MS.gene023274:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TATTAGGACTGATCATATTG+AGG	+	chr4.3:17455985-17456004	MS.gene023274:CDS	30.0%
!!	TGATCATATTGAGGTTGATA+AGG	+	chr4.3:17455994-17456013	MS.gene023274:CDS	30.0%
!!!	GAAAATCCAGTGTCATTTTA+GGG	-	chr4.3:17455781-17455800	None:intergenic	30.0%
!!!	TGAAAATCCAGTGTCATTTT+AGG	-	chr4.3:17455782-17455801	None:intergenic	30.0%
	AATTTCATTGAAGCTGCAAG+AGG	+	chr4.3:17455919-17455938	MS.gene023274:CDS	35.0%
	TTCAATGAAATTCCACGAAC+AGG	-	chr4.3:17455911-17455930	None:intergenic	35.0%
	TTCCTGAAGTTTCTGCTATT+AGG	+	chr4.3:17455969-17455988	MS.gene023274:CDS	35.0%
	TTCTTCAAGGATCTTCTTGT+TGG	-	chr4.3:17455807-17455826	None:intergenic	35.0%
!!!	ATTTCTTCACGGTTTTGGTT+CGG	-	chr4.3:17455730-17455749	None:intergenic	35.0%
	GTCCTAATAGCAGAAACTTC+AGG	-	chr4.3:17455974-17455993	None:intergenic	40.0%
	TAACCACCCTAAAATGACAC+TGG	+	chr4.3:17455772-17455791	MS.gene023274:CDS	40.0%
	TTGACGAGAGGATTTCTTCA+CGG	-	chr4.3:17455741-17455760	None:intergenic	40.0%
	TTGTTACGAAGACGCTTTGA+AGG	-	chr4.3:17455842-17455861	None:intergenic	40.0%
!	AGAGGATTTCTTCACGGTTT+TGG	-	chr4.3:17455735-17455754	None:intergenic	40.0%
!	TGTTACGAAGACGCTTTGAA+GGG	-	chr4.3:17455841-17455860	None:intergenic	40.0%
!!!	AATCCAGTGTCATTTTAGGG+TGG	-	chr4.3:17455778-17455797	None:intergenic	40.0%
	AAGAGCAGCAAGCATGTCTA+GGG	-	chr4.3:17456023-17456042	None:intergenic	45.0%
	CGTCTTCGTAACAAGATTGC+TGG	+	chr4.3:17455848-17455867	MS.gene023274:CDS	45.0%
	CTCATGCAGCGTATTCGAAA+GGG	+	chr4.3:17455884-17455903	MS.gene023274:CDS	45.0%
	GGATCAAAGCGACTTCTTCA+AGG	-	chr4.3:17455820-17455839	None:intergenic	45.0%
	TCTCATGCAGCGTATTCGAA+AGG	+	chr4.3:17455883-17455902	MS.gene023274:CDS	45.0%
	TTCATTGAAGCTGCAAGAGG+AGG	+	chr4.3:17455922-17455941	MS.gene023274:CDS	45.0%
!!	GAAGATTCCTGGTGTTGTTC+AGG	+	chr4.3:17456051-17456070	MS.gene023274:CDS	45.0%
	ACGCTCGATGATTTGACGAG+AGG	-	chr4.3:17455753-17455772	None:intergenic	50.0%
	ATTCGAAAGGGACCTGTTCG+TGG	+	chr4.3:17455896-17455915	MS.gene023274:CDS	50.0%
	CAAGAGCAGCAAGCATGTCT+AGG	-	chr4.3:17456024-17456043	None:intergenic	50.0%
	CCATATGGGATCTGCTGAAC+AGG	-	chr4.3:17456088-17456107	None:intergenic	50.0%
	CCTGTTCAGCAGATCCCATA+TGG	+	chr4.3:17456085-17456104	MS.gene023274:CDS	50.0%
	CGATCAACCTGAACAACACC+AGG	-	chr4.3:17456061-17456080	None:intergenic	50.0%
	CTTGGCATGTCGAAGATTCC+TGG	+	chr4.3:17456040-17456059	MS.gene023274:CDS	50.0%
!	CTAGACATGCTTGCTGCTCT+TGG	+	chr4.3:17456022-17456041	MS.gene023274:CDS	50.0%
	ATCCCATATGGTCGTGGTGC+TGG	+	chr4.3:17456097-17456116	MS.gene023274:CDS	55.0%
	CACCAGCACCACGACCATAT+GGG	-	chr4.3:17456102-17456121	None:intergenic	55.0%
	CAGCAGATCCCATATGGTCG+TGG	+	chr4.3:17456091-17456110	MS.gene023274:CDS	55.0%
	GCACCAGCACCACGACCATA+TGG	-	chr4.3:17456103-17456122	None:intergenic	60.0%
!	GGAGGAGCGTGATCGTCGTA+TGG	+	chr4.3:17455940-17455959	MS.gene023274:CDS	60.0%
!!	TATGGTCGTGGTGCTGGTGC+TGG	+	chr4.3:17456103-17456122	MS.gene023274:CDS	60.0%
!!	GTCGTGGTGCTGGTGCTGGA+AGG	+	chr4.3:17456107-17456126	MS.gene023274:CDS	65.0%
!!	GTGGTGCTGGTGCTGGAAGG+AGG	+	chr4.3:17456110-17456129	MS.gene023274:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	17455716	17456138	17455716	ID=MS.gene023274
chr4.3	mRNA	17455716	17456138	17455716	ID=MS.gene023274.t1;Parent=MS.gene023274
chr4.3	exon	17455716	17456138	17455716	ID=MS.gene023274.t1.exon1;Parent=MS.gene023274.t1
chr4.3	CDS	17455716	17456138	17455716	ID=cds.MS.gene023274.t1;Parent=MS.gene023274.t1

Gene Sequence

>MS.gene023274

ATGGGACGAGTCCGAACCAAAACCGTGAAGAAATCCTCTCGTCAAATCATCGAGCGTAACCACCCTAAAATGACACTGGATTTTCACACCAACAAGAAGATCCTTGAAGAAGTCGCTTTGATCCCTTCAAAGCGTCTTCGTAACAAGATTGCTGGTTTCTCTACGCATCTCATGCAGCGTATTCGAAAGGGACCTGTTCGTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGATCGTCGTATGGATTTCGTTCCTGAAGTTTCTGCTATTAGGACTGATCATATTGAGGTTGATAAGGAAACCCTAGACATGCTTGCTGCTCTTGGCATGTCGAAGATTCCTGGTGTTGTTCAGGTTGATCGTGTTCCTGTTCAGCAGATCCCATATGGTCGTGGTGCTGGTGCTGGAAGGAGGTTTTAA

Protein sequence

>MS.gene023274.t1

MGRVRTKTVKKSSRQIIERNHPKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMQRIRKGPVRGISLKLQEEERDRRMDFVPEVSAIRTDHIEVDKETLDMLAALGMSKIPGVVQVDRVPVQQIPYGRGAGAGRRF