Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065715.t1 | XP_013467927.1 | 98.4 | 127 | 2 | 0 | 1 | 127 | 1 | 127 | 1.70E-60 | 241.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065715.t1 | P49215 | 90.6 | 127 | 10 | 1 | 1 | 125 | 1 | 127 | 1.5e-55 | 216.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065715.t1 | I3SPL5 | 98.4 | 127 | 2 | 0 | 1 | 127 | 1 | 127 | 1.3e-60 | 241.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050290 | MS.gene065715 | 0.831733 | 1.41E-55 | -1.69E-46 |
| MS.gene050838 | MS.gene065715 | 0.819345 | 1.22E-52 | -1.69E-46 |
| MS.gene051330 | MS.gene065715 | 0.812787 | 3.56E-51 | -1.69E-46 |
| MS.gene051746 | MS.gene065715 | 0.837074 | 6.44E-57 | -1.69E-46 |
| MS.gene051748 | MS.gene065715 | 0.815809 | 7.64E-52 | -1.69E-46 |
| MS.gene051850 | MS.gene065715 | 0.859399 | 4.25E-63 | -1.69E-46 |
| MS.gene051903 | MS.gene065715 | 0.866171 | 3.46E-65 | -1.69E-46 |
| MS.gene052947 | MS.gene065715 | 0.866993 | 1.90E-65 | -1.69E-46 |
| MS.gene053952 | MS.gene065715 | 0.822338 | 2.50E-53 | -1.69E-46 |
| MS.gene054937 | MS.gene065715 | 0.879566 | 1.12E-69 | -1.69E-46 |
| MS.gene056075 | MS.gene065715 | 0.919749 | 3.05E-87 | -1.69E-46 |
| MS.gene056917 | MS.gene065715 | 0.892761 | 1.18E-74 | -1.69E-46 |
| MS.gene057092 | MS.gene065715 | 0.816061 | 6.71E-52 | -1.69E-46 |
| MS.gene057094 | MS.gene065715 | 0.839031 | 2.02E-57 | -1.69E-46 |
| MS.gene057705 | MS.gene065715 | 0.801598 | 8.35E-49 | -1.69E-46 |
| MS.gene058354 | MS.gene065715 | 0.871384 | 7.12E-67 | -1.69E-46 |
| MS.gene059227 | MS.gene065715 | 0.809589 | 1.76E-50 | -1.69E-46 |
| MS.gene059726 | MS.gene065715 | 0.808051 | 3.74E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene065715 | 0.871967 | 4.56E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene065715 | MS.gene023274 | PPI |
| MS.gene065715 | MS.gene023275 | PPI |
| MS.gene034834 | MS.gene065715 | PPI |
| MS.gene028177 | MS.gene065715 | PPI |
| MS.gene065715 | MS.gene039073 | PPI |
| MS.gene065715 | MS.gene028176 | PPI |
| MS.gene065715 | MS.gene039072 | PPI |
| MS.gene08344 | MS.gene065715 | PPI |
| MS.gene065715 | MS.gene54136 | PPI |
| MS.gene065715 | MS.gene056917 | PPI |
| MS.gene065715 | MS.gene34957 | PPI |
| MS.gene034832 | MS.gene065715 | PPI |
| MS.gene065715 | MS.gene08343 | PPI |
| MS.gene08343 | MS.gene065715 | PPI |
| MS.gene34958 | MS.gene065715 | PPI |
| MS.gene065715 | MS.gene08344 | PPI |
| MS.gene065715 | MS.gene040536 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065715.t1 | MTR_1g058250 | 98.425 | 127 | 2 | 0 | 1 | 127 | 1 | 127 | 7.48e-89 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065715.t1 | AT5G04800 | 81.955 | 133 | 20 | 1 | 1 | 129 | 1 | 133 | 1.10e-76 | 224 |
| MS.gene065715.t1 | AT5G04800 | 81.955 | 133 | 20 | 1 | 1 | 129 | 1 | 133 | 1.10e-76 | 224 |
| MS.gene065715.t1 | AT5G04800 | 81.955 | 133 | 20 | 1 | 1 | 129 | 1 | 133 | 1.10e-76 | 224 |
| MS.gene065715.t1 | AT5G04800 | 81.955 | 133 | 20 | 1 | 1 | 129 | 1 | 133 | 1.10e-76 | 224 |
| MS.gene065715.t1 | AT2G05220 | 81.203 | 133 | 21 | 1 | 1 | 129 | 1 | 133 | 1.64e-75 | 221 |
| MS.gene065715.t1 | AT2G05220 | 81.203 | 133 | 21 | 1 | 1 | 129 | 1 | 133 | 1.64e-75 | 221 |
| MS.gene065715.t1 | AT3G10610 | 86.777 | 121 | 16 | 0 | 1 | 121 | 1 | 121 | 1.79e-74 | 218 |
| MS.gene065715.t1 | AT2G04390 | 80.451 | 133 | 22 | 1 | 1 | 129 | 1 | 133 | 4.59e-74 | 217 |
Find 33 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGATTCCCGGGGTTGTTC+AGG | 0.315547 | 1.2:-45739548 | MS.gene065715:CDS |
| TTTGAATCCTCTTCATCAAA+TGG | 0.339004 | 1.2:+45739719 | None:intergenic |
| AGTAGTAACGTTCGATTACT+TGG | 0.348773 | 1.2:+45739842 | None:intergenic |
| TTGTTGCGAAGACGCTTTGA+TGG | 0.348895 | 1.2:+45739760 | None:intergenic |
| CCAAACGGAACCTGCTGAAC+AGG | 0.358474 | 1.2:+45739514 | None:intergenic |
| CTTGATATGCTTGCTGCTCT+TGG | 0.359734 | 1.2:-45739577 | MS.gene065715:CDS |
| CTTGGTATGTCCGAGATTCC+CGG | 0.369339 | 1.2:-45739559 | MS.gene065715:CDS |
| TCTCCTTATCAACCTCAATA+TGG | 0.414231 | 1.2:+45739602 | None:intergenic |
| TTGGTATGTCCGAGATTCCC+GGG | 0.419949 | 1.2:-45739558 | MS.gene065715:CDS |
| GAATCAATGCAACTTCTTCA+AGG | 0.430500 | 1.2:+45739782 | None:intergenic |
| TTGAATCCTCTTCATCAAAT+GGG | 0.440060 | 1.2:+45739720 | None:intergenic |
| TTTGATGAAGAGGATTCAAA+AGG | 0.440304 | 1.2:-45739716 | MS.gene065715:CDS |
| TGAACCTGTTCCTGTTCAGC+AGG | 0.453615 | 1.2:-45739524 | MS.gene065715:intron |
| GGAAGAGCGTGAGAGGAGGA+TGG | 0.458231 | 1.2:-45739659 | MS.gene065715:CDS |
| TTTGTTGGTGTGGAAATCTA+GGG | 0.464737 | 1.2:+45739810 | None:intergenic |
| GGAACCTGCTGAACAGGAAC+AGG | 0.466414 | 1.2:+45739520 | None:intergenic |
| TGACCATATTGAGGTTGATA+AGG | 0.472007 | 1.2:-45739605 | MS.gene065715:CDS |
| CTGAACAACCCCGGGAATCT+CGG | 0.484648 | 1.2:+45739549 | None:intergenic |
| ATTCAAAAGGGACCGGTTCG+TGG | 0.491412 | 1.2:-45739703 | MS.gene065715:CDS |
| TTTCAACCCATTTGATGAAG+AGG | 0.506012 | 1.2:-45739726 | MS.gene065715:CDS |
| CGTCTTCGCAACAAGATCGC+TGG | 0.529414 | 1.2:-45739751 | MS.gene065715:CDS |
| TTCAACGAGATTCCACGAAC+CGG | 0.549035 | 1.2:+45739691 | None:intergenic |
| TACAAGAGGAAGAGCGTGAG+AGG | 0.560260 | 1.2:-45739666 | MS.gene065715:CDS |
| TTGATGAAGAGGATTCAAAA+GGG | 0.572781 | 1.2:-45739715 | MS.gene065715:CDS |
| GAAGAGGATTCAAAAGGGAC+CGG | 0.577199 | 1.2:-45739710 | MS.gene065715:CDS |
| GTTCTGATAGCAGAAACATC+AGG | 0.577897 | 1.2:+45739628 | None:intergenic |
| TATCAGAACTGACCATATTG+AGG | 0.583233 | 1.2:-45739614 | MS.gene065715:CDS |
| AAGAGGAAGAGCGTGAGAGG+AGG | 0.595347 | 1.2:-45739663 | MS.gene065715:CDS |
| AGGTTCAGCCTGAACAACCC+CGG | 0.604387 | 1.2:+45739540 | None:intergenic |
| GGTTCAGCCTGAACAACCCC+GGG | 0.607828 | 1.2:+45739541 | None:intergenic |
| TGGTATGTCCGAGATTCCCG+GGG | 0.623039 | 1.2:-45739557 | MS.gene065715:CDS |
| ACGTTCGATTACTTGGCGTG+AGG | 0.642792 | 1.2:+45739849 | None:intergenic |
| AATCTCGTTGAAGCTACAAG+AGG | 0.693243 | 1.2:-45739680 | MS.gene065715:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTTGTTAAGTTGTTGTTAT+GGG | - | chr1.2:45739835-45739854 | MS.gene065715:CDS | 25.0% |
| !!! | AACTTGTTAAGTTGTTGTTA+TGG | - | chr1.2:45739834-45739853 | MS.gene065715:CDS | 25.0% |
| !!! | TTCTTCAAGGATTTTTTTGT+TGG | + | chr1.2:45739418-45739437 | None:intergenic | 25.0% |
| TTGAATCCTCTTCATCAAAT+GGG | + | chr1.2:45739493-45739512 | None:intergenic | 30.0% | |
| TTGATGAAGAGGATTCAAAA+GGG | - | chr1.2:45739495-45739514 | MS.gene065715:intron | 30.0% | |
| TTTGAATCCTCTTCATCAAA+TGG | + | chr1.2:45739494-45739513 | None:intergenic | 30.0% | |
| TTTGATGAAGAGGATTCAAA+AGG | - | chr1.2:45739494-45739513 | MS.gene065715:intron | 30.0% | |
| AGTAGTAACGTTCGATTACT+TGG | + | chr1.2:45739371-45739390 | None:intergenic | 35.0% | |
| GAATCAATGCAACTTCTTCA+AGG | + | chr1.2:45739431-45739450 | None:intergenic | 35.0% | |
| TATCAGAACTGACCATATTG+AGG | - | chr1.2:45739596-45739615 | MS.gene065715:CDS | 35.0% | |
| TCTCCTTATCAACCTCAATA+TGG | + | chr1.2:45739611-45739630 | None:intergenic | 35.0% | |
| TTTCAACCCATTTGATGAAG+AGG | - | chr1.2:45739484-45739503 | MS.gene065715:intron | 35.0% | |
| TTTGTTGGTGTGGAAATCTA+GGG | + | chr1.2:45739403-45739422 | None:intergenic | 35.0% | |
| ! | TTTTGTTGGTGTGGAAATCT+AGG | + | chr1.2:45739404-45739423 | None:intergenic | 35.0% |
| !! | TGACCATATTGAGGTTGATA+AGG | - | chr1.2:45739605-45739624 | MS.gene065715:CDS | 35.0% |
| !!! | CAAGGATTTTTTTGTTGGTG+TGG | + | chr1.2:45739413-45739432 | None:intergenic | 35.0% |
| !!! | TGAGGATTTTTTCACGGTTT+TGG | + | chr1.2:45739346-45739365 | None:intergenic | 35.0% |
| AATCTCGTTGAAGCTACAAG+AGG | - | chr1.2:45739530-45739549 | MS.gene065715:CDS | 40.0% | |
| GTTCTGATAGCAGAAACATC+AGG | + | chr1.2:45739585-45739604 | None:intergenic | 40.0% | |
| !!! | TTGGCGTGAGGATTTTTTCA+CGG | + | chr1.2:45739352-45739371 | None:intergenic | 40.0% |
| GAAGAGGATTCAAAAGGGAC+CGG | - | chr1.2:45739500-45739519 | MS.gene065715:intron | 45.0% | |
| TTCAACGAGATTCCACGAAC+CGG | + | chr1.2:45739522-45739541 | None:intergenic | 45.0% | |
| TTGTTGCGAAGACGCTTTGA+TGG | + | chr1.2:45739453-45739472 | None:intergenic | 45.0% | |
| ! | CTTGATATGCTTGCTGCTCT+TGG | - | chr1.2:45739633-45739652 | MS.gene065715:CDS | 45.0% |
| ACACCAGCACCTCTTCCAAA+CGG | + | chr1.2:45739714-45739733 | None:intergenic | 50.0% | |
| ACGTTCGATTACTTGGCGTG+AGG | + | chr1.2:45739364-45739383 | None:intergenic | 50.0% | |
| CTTGGTATGTCCGAGATTCC+CGG | - | chr1.2:45739651-45739670 | MS.gene065715:CDS | 50.0% | |
| TGAACCTGTTCCTGTTCAGC+AGG | - | chr1.2:45739686-45739705 | MS.gene065715:CDS | 50.0% | |
| TTGGTATGTCCGAGATTCCC+GGG | - | chr1.2:45739652-45739671 | MS.gene065715:CDS | 50.0% | |
| ! | TACAAGAGGAAGAGCGTGAG+AGG | - | chr1.2:45739544-45739563 | MS.gene065715:CDS | 50.0% |
| !! | ATTCAAAAGGGACCGGTTCG+TGG | - | chr1.2:45739507-45739526 | MS.gene065715:intron | 50.0% |
| AGGTTCAGCCTGAACAACCC+CGG | + | chr1.2:45739673-45739692 | None:intergenic | 55.0% | |
| CAGCAGGTTCCGTTTGGAAG+AGG | - | chr1.2:45739702-45739721 | MS.gene065715:CDS | 55.0% | |
| CCAAACGGAACCTGCTGAAC+AGG | + | chr1.2:45739699-45739718 | None:intergenic | 55.0% | |
| CCTGTTCAGCAGGTTCCGTT+TGG | - | chr1.2:45739696-45739715 | MS.gene065715:CDS | 55.0% | |
| CGTCTTCGCAACAAGATCGC+TGG | - | chr1.2:45739459-45739478 | MS.gene065715:intron | 55.0% | |
| CTGAACAACCCCGGGAATCT+CGG | + | chr1.2:45739664-45739683 | None:intergenic | 55.0% | |
| GGAACCTGCTGAACAGGAAC+AGG | + | chr1.2:45739693-45739712 | None:intergenic | 55.0% | |
| TGGTATGTCCGAGATTCCCG+GGG | - | chr1.2:45739653-45739672 | MS.gene065715:CDS | 55.0% | |
| ! | AAGAGGAAGAGCGTGAGAGG+AGG | - | chr1.2:45739547-45739566 | MS.gene065715:CDS | 55.0% |
| ! | GTTCCGTTTGGAAGAGGTGC+TGG | - | chr1.2:45739708-45739727 | MS.gene065715:CDS | 55.0% |
| !! | TTGCTGCTGGTGGTGGAAGA+AGG | - | chr1.2:45739736-45739755 | MS.gene065715:CDS | 55.0% |
| CGAGATTCCCGGGGTTGTTC+AGG | - | chr1.2:45739662-45739681 | MS.gene065715:CDS | 60.0% | |
| GGTTCAGCCTGAACAACCCC+GGG | + | chr1.2:45739672-45739691 | None:intergenic | 60.0% | |
| ! | GGAAGAGCGTGAGAGGAGGA+TGG | - | chr1.2:45739551-45739570 | MS.gene065715:CDS | 60.0% |
| !! | GCTGGTGTTGTTGCTGCTGG+TGG | - | chr1.2:45739726-45739745 | MS.gene065715:CDS | 60.0% |
| !! | GGTGCTGGTGTTGTTGCTGC+TGG | - | chr1.2:45739723-45739742 | MS.gene065715:CDS | 60.0% |
| !! | GGTGTTGTTGCTGCTGGTGG+TGG | - | chr1.2:45739729-45739748 | MS.gene065715:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 45739327 | 45739905 | 45739327 | ID=MS.gene065715 |
| chr1.2 | mRNA | 45739327 | 45739905 | 45739327 | ID=MS.gene065715.t1;Parent=MS.gene065715 |
| chr1.2 | exon | 45739525 | 45739905 | 45739525 | ID=MS.gene065715.t1.exon1;Parent=MS.gene065715.t1 |
| chr1.2 | CDS | 45739525 | 45739905 | 45739525 | ID=cds.MS.gene065715.t1;Parent=MS.gene065715.t1 |
| chr1.2 | exon | 45739327 | 45739359 | 45739327 | ID=MS.gene065715.t1.exon2;Parent=MS.gene065715.t1 |
| chr1.2 | CDS | 45739327 | 45739359 | 45739327 | ID=cds.MS.gene065715.t1;Parent=MS.gene065715.t1 |
| Gene Sequence |
| Protein sequence |
