AlfalfaGEDB | MS.gene034834

M. sativa cultivar XinJiangDaYe / MS.gene034834

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034834.t1	XP_013467927.1	97.9	145	2	1	1	145	1	144	1.10E-68	269.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034834.t1	P49215	83.1	148	18	3	1	145	1	144	9.6e-58	224.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034834.t1	I3SPL5	97.9	145	2	1	1	145	1	144	7.8e-69	269.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene034834	MS.gene040536	PPI
MS.gene034834	MS.gene54136	PPI
MS.gene028177	MS.gene034834	PPI
MS.gene034834	MS.gene34957	PPI
MS.gene034834	MS.gene08343	PPI
MS.gene034834	MS.gene08344	PPI
MS.gene034834	MS.gene023274	PPI
MS.gene034834	MS.gene065715	PPI
MS.gene034834	MS.gene023275	PPI
MS.gene034832	MS.gene034834	PPI
MS.gene34958	MS.gene034834	PPI
MS.gene034834	MS.gene056917	PPI
MS.gene034834	MS.gene039073	PPI
MS.gene034834	MS.gene034832	PPI
MS.gene034834	MS.gene028176	PPI
MS.gene034834	MS.gene039072	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034834.t1	MTR_1g058250	97.931	145	2	1	1	145	1	144	2.85e-100	284

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034834.t1	AT5G04800	80.420	143	21	2	1	143	1	136	4.35e-77	225
MS.gene034834.t1	AT5G04800	80.420	143	21	2	1	143	1	136	4.35e-77	225
MS.gene034834.t1	AT5G04800	80.420	143	21	2	1	143	1	136	4.35e-77	225
MS.gene034834.t1	AT5G04800	80.420	143	21	2	1	143	1	136	4.35e-77	225
MS.gene034834.t1	AT2G05220	79.021	143	22	2	1	143	1	135	2.81e-75	221
MS.gene034834.t1	AT2G05220	79.021	143	22	2	1	143	1	135	2.81e-75	221
MS.gene034834.t1	AT3G10610	80.576	139	20	2	1	135	1	136	8.41e-75	219
MS.gene034834.t1	AT2G04390	79.021	143	23	2	1	143	1	136	1.98e-74	218

Find 41 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGAGATTCCCGGGGTTGTTC+AGG	0.315547	1.4:+48538873	MS.gene034834:CDS
TTTGAATCCTCTTCATCAAA+TGG	0.339004	1.4:-48538702	None:intergenic
AGTAGTAACGTTCGATTACT+TGG	0.348773	1.4:-48538579	None:intergenic
TTGTTGCGAAGACGCTTTGA+TGG	0.348895	1.4:-48538661	None:intergenic
CCAAACGGAACCTGCTGAAC+AGG	0.358474	1.4:-48538907	None:intergenic
CTTGATATGCTTGCTGCTCT+TGG	0.359734	1.4:+48538844	MS.gene034834:CDS
CTTGGTATGTCCGAGATTCC+CGG	0.369339	1.4:+48538862	MS.gene034834:CDS
GGTGTTGTTGCTGCTGGTGG+TGG	0.407972	1.4:+48538940	MS.gene034834:CDS
TCTCCTTATCAACCTCAATA+TGG	0.414231	1.4:-48538819	None:intergenic
TTGGTATGTCCGAGATTCCC+GGG	0.419949	1.4:+48538863	MS.gene034834:CDS
TTGCTGCTGGTGGTGGAAGA+AGG	0.424565	1.4:+48538947	MS.gene034834:CDS
GAATCAATGCAACTTCTTCA+AGG	0.430500	1.4:-48538639	None:intergenic
TTGAATCCTCTTCATCAAAT+GGG	0.440060	1.4:-48538701	None:intergenic
TTTGATGAAGAGGATTCAAA+AGG	0.440304	1.4:+48538705	MS.gene034834:CDS
CCTGTTCAGCAGGTTCCGTT+TGG	0.441092	1.4:+48538907	MS.gene034834:CDS
CAGCAGGTTCCGTTTGGAAG+AGG	0.445053	1.4:+48538913	MS.gene034834:CDS
GGTGCTGGTGTTGTTGCTGC+TGG	0.451152	1.4:+48538934	MS.gene034834:CDS
TGAACCTGTTCCTGTTCAGC+AGG	0.453615	1.4:+48538897	MS.gene034834:CDS
GGAAGAGCGTGAGAGGAGGA+TGG	0.458231	1.4:+48538762	MS.gene034834:CDS
TTTGTTGGTGTGGAAATCTA+GGG	0.464737	1.4:-48538611	None:intergenic
GTTCCGTTTGGAAGAGGTGC+TGG	0.465201	1.4:+48538919	MS.gene034834:CDS
GGAACCTGCTGAACAGGAAC+AGG	0.466414	1.4:-48538901	None:intergenic
TGACCATATTGAGGTTGATA+AGG	0.472007	1.4:+48538816	MS.gene034834:CDS
CTGAACAACCCCGGGAATCT+CGG	0.484648	1.4:-48538872	None:intergenic
ATTCAAAAGGGACCGGTTCG+TGG	0.491412	1.4:+48538718	MS.gene034834:CDS
TTTCAACCCATTTGATGAAG+AGG	0.506012	1.4:+48538695	MS.gene034834:CDS
GCTGGTGTTGTTGCTGCTGG+TGG	0.527362	1.4:+48538937	MS.gene034834:CDS
CGTCTTCGCAACAAGATCGC+TGG	0.529414	1.4:+48538670	MS.gene034834:CDS
TTCAACGAGATTCCACGAAC+CGG	0.549035	1.4:-48538730	None:intergenic
TACAAGAGGAAGAGCGTGAG+AGG	0.560260	1.4:+48538755	MS.gene034834:CDS
ACACCAGCACCTCTTCCAAA+CGG	0.569681	1.4:-48538922	None:intergenic
TTGATGAAGAGGATTCAAAA+GGG	0.572781	1.4:+48538706	MS.gene034834:CDS
GAAGAGGATTCAAAAGGGAC+CGG	0.577199	1.4:+48538711	MS.gene034834:CDS
GTTCTGATAGCAGAAACATC+AGG	0.577897	1.4:-48538793	None:intergenic
TATCAGAACTGACCATATTG+AGG	0.583233	1.4:+48538807	MS.gene034834:CDS
AAGAGGAAGAGCGTGAGAGG+AGG	0.595347	1.4:+48538758	MS.gene034834:CDS
AGGTTCAGCCTGAACAACCC+CGG	0.604387	1.4:-48538881	None:intergenic
GGTTCAGCCTGAACAACCCC+GGG	0.607828	1.4:-48538880	None:intergenic
TGGTATGTCCGAGATTCCCG+GGG	0.623039	1.4:+48538864	MS.gene034834:CDS
ACGTTCGATTACTTGGCGTG+AGG	0.642792	1.4:-48538572	None:intergenic
AATCTCGTTGAAGCTACAAG+AGG	0.693243	1.4:+48538741	MS.gene034834:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTTCAAGGATTTTTTTGT+TGG	-	chr1.4:48538629-48538648	None:intergenic	25.0%
	TTGAATCCTCTTCATCAAAT+GGG	-	chr1.4:48538704-48538723	None:intergenic	30.0%
	TTGATGAAGAGGATTCAAAA+GGG	+	chr1.4:48538706-48538725	MS.gene034834:CDS	30.0%
	TTTGAATCCTCTTCATCAAA+TGG	-	chr1.4:48538705-48538724	None:intergenic	30.0%
	TTTGATGAAGAGGATTCAAA+AGG	+	chr1.4:48538705-48538724	MS.gene034834:CDS	30.0%
	AGTAGTAACGTTCGATTACT+TGG	-	chr1.4:48538582-48538601	None:intergenic	35.0%
	GAATCAATGCAACTTCTTCA+AGG	-	chr1.4:48538642-48538661	None:intergenic	35.0%
	TATCAGAACTGACCATATTG+AGG	+	chr1.4:48538807-48538826	MS.gene034834:CDS	35.0%
	TCTCCTTATCAACCTCAATA+TGG	-	chr1.4:48538822-48538841	None:intergenic	35.0%
	TTTCAACCCATTTGATGAAG+AGG	+	chr1.4:48538695-48538714	MS.gene034834:CDS	35.0%
	TTTGTTGGTGTGGAAATCTA+GGG	-	chr1.4:48538614-48538633	None:intergenic	35.0%
!	TTTTGTTGGTGTGGAAATCT+AGG	-	chr1.4:48538615-48538634	None:intergenic	35.0%
!!	TGACCATATTGAGGTTGATA+AGG	+	chr1.4:48538816-48538835	MS.gene034834:CDS	35.0%
!!!	CAAGGATTTTTTTGTTGGTG+TGG	-	chr1.4:48538624-48538643	None:intergenic	35.0%
!!!	TGAGGATTTTTTCACGGTTT+TGG	-	chr1.4:48538557-48538576	None:intergenic	35.0%
	AATCTCGTTGAAGCTACAAG+AGG	+	chr1.4:48538741-48538760	MS.gene034834:CDS	40.0%
	GTTCTGATAGCAGAAACATC+AGG	-	chr1.4:48538796-48538815	None:intergenic	40.0%
!!!	TTGGCGTGAGGATTTTTTCA+CGG	-	chr1.4:48538563-48538582	None:intergenic	40.0%
	GAAGAGGATTCAAAAGGGAC+CGG	+	chr1.4:48538711-48538730	MS.gene034834:CDS	45.0%
	TTCAACGAGATTCCACGAAC+CGG	-	chr1.4:48538733-48538752	None:intergenic	45.0%
	TTGTTGCGAAGACGCTTTGA+TGG	-	chr1.4:48538664-48538683	None:intergenic	45.0%
!	CTTGATATGCTTGCTGCTCT+TGG	+	chr1.4:48538844-48538863	MS.gene034834:CDS	45.0%
	ACACCAGCACCTCTTCCAAA+CGG	-	chr1.4:48538925-48538944	None:intergenic	50.0%
	ACGTTCGATTACTTGGCGTG+AGG	-	chr1.4:48538575-48538594	None:intergenic	50.0%
	CTTGGTATGTCCGAGATTCC+CGG	+	chr1.4:48538862-48538881	MS.gene034834:CDS	50.0%
	TGAACCTGTTCCTGTTCAGC+AGG	+	chr1.4:48538897-48538916	MS.gene034834:CDS	50.0%
	TTGGTATGTCCGAGATTCCC+GGG	+	chr1.4:48538863-48538882	MS.gene034834:CDS	50.0%
!	TACAAGAGGAAGAGCGTGAG+AGG	+	chr1.4:48538755-48538774	MS.gene034834:CDS	50.0%
!!	ATTCAAAAGGGACCGGTTCG+TGG	+	chr1.4:48538718-48538737	MS.gene034834:CDS	50.0%
	AGGTTCAGCCTGAACAACCC+CGG	-	chr1.4:48538884-48538903	None:intergenic	55.0%
	CAGCAGGTTCCGTTTGGAAG+AGG	+	chr1.4:48538913-48538932	MS.gene034834:CDS	55.0%
	CCAAACGGAACCTGCTGAAC+AGG	-	chr1.4:48538910-48538929	None:intergenic	55.0%
	CCTGTTCAGCAGGTTCCGTT+TGG	+	chr1.4:48538907-48538926	MS.gene034834:CDS	55.0%
	CGTCTTCGCAACAAGATCGC+TGG	+	chr1.4:48538670-48538689	MS.gene034834:CDS	55.0%
	CTGAACAACCCCGGGAATCT+CGG	-	chr1.4:48538875-48538894	None:intergenic	55.0%
	GGAACCTGCTGAACAGGAAC+AGG	-	chr1.4:48538904-48538923	None:intergenic	55.0%
	TGGTATGTCCGAGATTCCCG+GGG	+	chr1.4:48538864-48538883	MS.gene034834:CDS	55.0%
!	AAGAGGAAGAGCGTGAGAGG+AGG	+	chr1.4:48538758-48538777	MS.gene034834:CDS	55.0%
!	GTTCCGTTTGGAAGAGGTGC+TGG	+	chr1.4:48538919-48538938	MS.gene034834:CDS	55.0%
!!	TTGCTGCTGGTGGTGGAAGA+AGG	+	chr1.4:48538947-48538966	MS.gene034834:CDS	55.0%
	CGAGATTCCCGGGGTTGTTC+AGG	+	chr1.4:48538873-48538892	MS.gene034834:CDS	60.0%
	GGTTCAGCCTGAACAACCCC+GGG	-	chr1.4:48538883-48538902	None:intergenic	60.0%
!	GGAAGAGCGTGAGAGGAGGA+TGG	+	chr1.4:48538762-48538781	MS.gene034834:CDS	60.0%
!!	GCTGGTGTTGTTGCTGCTGG+TGG	+	chr1.4:48538937-48538956	MS.gene034834:CDS	60.0%
!!	GGTGCTGGTGTTGTTGCTGC+TGG	+	chr1.4:48538934-48538953	MS.gene034834:CDS	60.0%
!!	GGTGTTGTTGCTGCTGGTGG+TGG	+	chr1.4:48538940-48538959	MS.gene034834:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	48538538	48538975	48538538	ID=MS.gene034834
chr1.4	mRNA	48538538	48538975	48538538	ID=MS.gene034834.t1;Parent=MS.gene034834
chr1.4	exon	48538538	48538975	48538538	ID=MS.gene034834.t1.exon1;Parent=MS.gene034834.t1
chr1.4	CDS	48538538	48538975	48538538	ID=cds.MS.gene034834.t1;Parent=MS.gene034834.t1

Gene Sequence

>MS.gene034834

ATGGGTCGTGTCAGAACCAAAACCGTGAAAAAATCCTCACGCCAAGTAATCGAACGTTACTACTCAAAAATGACCCTAGATTTCCACACCAACAAAAAAATCCTTGAAGAAGTTGCATTGATTCCATCAAAGCGTCTTCGCAACAAGATCGCTGGATTTTCAACCCATTTGATGAAGAGGATTCAAAAGGGACCGGTTCGTGGAATCTCGTTGAAGCTACAAGAGGAAGAGCGTGAGAGGAGGATGGATTTCGTTCCTGATGTTTCTGCTATCAGAACTGACCATATTGAGGTTGATAAGGAGACTCTTGATATGCTTGCTGCTCTTGGTATGTCCGAGATTCCCGGGGTTGTTCAGGCTGAACCTGTTCCTGTTCAGCAGGTTCCGTTTGGAAGAGGTGCTGGTGTTGTTGCTGCTGGTGGTGGAAGAAGGTTTTGA

Protein sequence

>MS.gene034834.t1

MGRVRTKTVKKSSRQVIERYYSKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDHIEVDKETLDMLAALGMSEIPGVVQAEPVPVQQVPFGRGAGVVAAGGGRRF