AlfalfaGEDB | MS.gene08344

M. sativa cultivar XinJiangDaYe / MS.gene08344

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08344.t1	ACJ84115.1	89.6	144	11	1	1	140	1	144	3.30E-62	247.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08344.t1	P49215	78.5	144	27	3	1	140	1	144	4.0e-53	208.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08344.t1	B7FH86	89.6	144	11	1	1	140	1	144	2.3e-62	247.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene08344	MS.gene056917	PPI
MS.gene08344	MS.gene039073	PPI
MS.gene08344	MS.gene34957	PPI
MS.gene08344	MS.gene028176	PPI
MS.gene034834	MS.gene08344	PPI
MS.gene028177	MS.gene08344	PPI
MS.gene08344	MS.gene040536	PPI
MS.gene08344	MS.gene54136	PPI
MS.gene08344	MS.gene08343	PPI
MS.gene08344	MS.gene023274	PPI
MS.gene034832	MS.gene08344	PPI
MS.gene08343	MS.gene08344	PPI
MS.gene34958	MS.gene08344	PPI
MS.gene08344	MS.gene065715	PPI
MS.gene08344	MS.gene039072	PPI
MS.gene065715	MS.gene08344	PPI
MS.gene08344	MS.gene023275	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08344.t1	MTR_1g058250	93.103	116	8	0	1	116	1	116	3.76e-75	220

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08344.t1	AT5G04800	73.239	142	35	2	1	140	1	141	1.26e-69	206
MS.gene08344.t1	AT5G04800	73.239	142	35	2	1	140	1	141	1.26e-69	206
MS.gene08344.t1	AT5G04800	73.239	142	35	2	1	140	1	141	1.26e-69	206
MS.gene08344.t1	AT5G04800	73.239	142	35	2	1	140	1	141	1.26e-69	206
MS.gene08344.t1	AT3G10610	73.571	140	36	1	1	139	1	140	5.11e-69	204
MS.gene08344.t1	AT2G04390	73.759	141	36	1	1	140	1	141	1.10e-68	204
MS.gene08344.t1	AT2G05220	72.857	140	38	0	1	140	1	140	4.15e-68	202
MS.gene08344.t1	AT2G05220	72.857	140	38	0	1	140	1	140	4.15e-68	202

Find 35 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGGATTTCTTCACGGTTT+TGG	0.259892	4.1:-16950067	None:intergenic
TTCCTGATGTTTCTGCTATT+AGG	0.341443	4.1:+16950304	MS.gene08344:CDS
GAAGATTCCTGGTGTTGTTC+AGG	0.347999	4.1:+16950386	MS.gene08344:CDS
CGTCTTCGTAACAAGATTGC+TGG	0.356034	4.1:+16950183	MS.gene08344:CDS
CAAGAGCAGCAAGCATATCT+AGG	0.361317	4.1:-16950356	None:intergenic
CTTGGCATGTCGAAGATTCC+TGG	0.368757	4.1:+16950375	MS.gene08344:CDS
TTCTTCAAGGATCTTCTTGT+TGG	0.377402	4.1:-16950139	None:intergenic
CTAGATATGCTTGCTGCTCT+TGG	0.395976	4.1:+16950357	MS.gene08344:CDS
GCACCAGCACCACGACCATA+TGG	0.428749	4.1:-16950435	None:intergenic
ATCCCATATGGTCGTGGTGC+TGG	0.431174	4.1:+16950432	MS.gene08344:CDS
TATGGTCGTGGTGCTGGTGC+TGG	0.432732	4.1:+16950438	MS.gene08344:CDS
TTCAATGAAATTCCACGAAC+AGG	0.443778	4.1:-16950243	None:intergenic
TTGTTACGAAGACGCTTTGA+AGG	0.464587	4.1:-16950174	None:intergenic
GGATCAAAGCGACTTCTTCA+AGG	0.477079	4.1:-16950152	None:intergenic
CTTGTTCAGCAGATCCCATA+TGG	0.480117	4.1:+16950420	MS.gene08344:CDS
ATTCGAAAGGGACCTGTTCG+TGG	0.480777	4.1:+16950231	MS.gene08344:CDS
TGATCATATTGAGGTTGATA+AGG	0.481558	4.1:+16950329	MS.gene08344:CDS
GTCCTAATAGCAGAAACATC+AGG	0.505221	4.1:-16950306	None:intergenic
TTTGGTTCGGACTCGTCCCA+TGG	0.523667	4.1:-16950049	None:intergenic
TTGACGAGAGGATTTCTTCA+CGG	0.524605	4.1:-16950073	None:intergenic
GGAGGAGCGTGATCGTCGTA+TGG	0.525336	4.1:+16950275	MS.gene08344:CDS
TATTAGGACTGATCATATTG+AGG	0.542204	4.1:+16950320	MS.gene08344:CDS
GTGGTGCTGGTGCTGGAAGG+AGG	0.550588	4.1:+16950445	MS.gene08344:CDS
CACCAGCACCACGACCATAT+GGG	0.556729	4.1:-16950434	None:intergenic
CTCATGCAGCGTATTCGAAA+GGG	0.564195	4.1:+16950219	MS.gene08344:CDS
GTCGTGGTGCTGGTGCTGGA+AGG	0.566651	4.1:+16950442	MS.gene08344:CDS
TTCATTGAAGCTGCAAGAGG+AGG	0.567846	4.1:+16950257	MS.gene08344:CDS
CGATTAACCTGAACAACACC+AGG	0.574528	4.1:-16950393	None:intergenic
CAGCAGATCCCATATGGTCG+TGG	0.574554	4.1:+16950426	MS.gene08344:CDS
AAGAGCAGCAAGCATATCTA+GGG	0.580550	4.1:-16950355	None:intergenic
TCTCATGCAGCGTATTCGAA+AGG	0.588377	4.1:+16950218	MS.gene08344:CDS
TGTTACGAAGACGCTTTGAA+GGG	0.597110	4.1:-16950173	None:intergenic
ACGCTCGATGATTTGACGAG+AGG	0.611995	4.1:-16950085	None:intergenic
TAACCACCCTAAAATGACAC+TGG	0.617615	4.1:+16950107	MS.gene08344:CDS
AATTTCATTGAAGCTGCAAG+AGG	0.634258	4.1:+16950254	MS.gene08344:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TATTAGGACTGATCATATTG+AGG	+	chr4.1:16950320-16950339	MS.gene08344:CDS	30.0%
!!	TGATCATATTGAGGTTGATA+AGG	+	chr4.1:16950329-16950348	MS.gene08344:CDS	30.0%
!!!	GAAAATCCAGTGTCATTTTA+GGG	-	chr4.1:16950116-16950135	None:intergenic	30.0%
!!!	TGAAAATCCAGTGTCATTTT+AGG	-	chr4.1:16950117-16950136	None:intergenic	30.0%
	AATTTCATTGAAGCTGCAAG+AGG	+	chr4.1:16950254-16950273	MS.gene08344:CDS	35.0%
	TTCAATGAAATTCCACGAAC+AGG	-	chr4.1:16950246-16950265	None:intergenic	35.0%
	TTCTTCAAGGATCTTCTTGT+TGG	-	chr4.1:16950142-16950161	None:intergenic	35.0%
!	TTCCTGATGTTTCTGCTATT+AGG	+	chr4.1:16950304-16950323	MS.gene08344:CDS	35.0%
!!!	ATTTCTTCACGGTTTTGGTT+CGG	-	chr4.1:16950065-16950084	None:intergenic	35.0%
	AAGAGCAGCAAGCATATCTA+GGG	-	chr4.1:16950358-16950377	None:intergenic	40.0%
	GTCCTAATAGCAGAAACATC+AGG	-	chr4.1:16950309-16950328	None:intergenic	40.0%
	TAACCACCCTAAAATGACAC+TGG	+	chr4.1:16950107-16950126	MS.gene08344:CDS	40.0%
	TTGACGAGAGGATTTCTTCA+CGG	-	chr4.1:16950076-16950095	None:intergenic	40.0%
	TTGTTACGAAGACGCTTTGA+AGG	-	chr4.1:16950177-16950196	None:intergenic	40.0%
!	AGAGGATTTCTTCACGGTTT+TGG	-	chr4.1:16950070-16950089	None:intergenic	40.0%
!	TGTTACGAAGACGCTTTGAA+GGG	-	chr4.1:16950176-16950195	None:intergenic	40.0%
!!!	AATCCAGTGTCATTTTAGGG+TGG	-	chr4.1:16950113-16950132	None:intergenic	40.0%
	CAAGAGCAGCAAGCATATCT+AGG	-	chr4.1:16950359-16950378	None:intergenic	45.0%
	CGATTAACCTGAACAACACC+AGG	-	chr4.1:16950396-16950415	None:intergenic	45.0%
	CGTCTTCGTAACAAGATTGC+TGG	+	chr4.1:16950183-16950202	MS.gene08344:CDS	45.0%
	CTCATGCAGCGTATTCGAAA+GGG	+	chr4.1:16950219-16950238	MS.gene08344:CDS	45.0%
	CTTGTTCAGCAGATCCCATA+TGG	+	chr4.1:16950420-16950439	MS.gene08344:CDS	45.0%
	GGATCAAAGCGACTTCTTCA+AGG	-	chr4.1:16950155-16950174	None:intergenic	45.0%
	TCTCATGCAGCGTATTCGAA+AGG	+	chr4.1:16950218-16950237	MS.gene08344:CDS	45.0%
	TTCATTGAAGCTGCAAGAGG+AGG	+	chr4.1:16950257-16950276	MS.gene08344:CDS	45.0%
!	CTAGATATGCTTGCTGCTCT+TGG	+	chr4.1:16950357-16950376	MS.gene08344:CDS	45.0%
!!	GAAGATTCCTGGTGTTGTTC+AGG	+	chr4.1:16950386-16950405	MS.gene08344:CDS	45.0%
	ACGCTCGATGATTTGACGAG+AGG	-	chr4.1:16950088-16950107	None:intergenic	50.0%
	ATTCGAAAGGGACCTGTTCG+TGG	+	chr4.1:16950231-16950250	MS.gene08344:CDS	50.0%
	CTTGGCATGTCGAAGATTCC+TGG	+	chr4.1:16950375-16950394	MS.gene08344:CDS	50.0%
	ATCCCATATGGTCGTGGTGC+TGG	+	chr4.1:16950432-16950451	MS.gene08344:CDS	55.0%
	CACCAGCACCACGACCATAT+GGG	-	chr4.1:16950437-16950456	None:intergenic	55.0%
	CAGCAGATCCCATATGGTCG+TGG	+	chr4.1:16950426-16950445	MS.gene08344:CDS	55.0%
	GCACCAGCACCACGACCATA+TGG	-	chr4.1:16950438-16950457	None:intergenic	60.0%
!	GGAGGAGCGTGATCGTCGTA+TGG	+	chr4.1:16950275-16950294	MS.gene08344:CDS	60.0%
!!	TATGGTCGTGGTGCTGGTGC+TGG	+	chr4.1:16950438-16950457	MS.gene08344:CDS	60.0%
!!	GTCGTGGTGCTGGTGCTGGA+AGG	+	chr4.1:16950442-16950461	MS.gene08344:CDS	65.0%
!!	GTGGTGCTGGTGCTGGAAGG+AGG	+	chr4.1:16950445-16950464	MS.gene08344:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	16950051	16950473	16950051	ID=MS.gene08344
chr4.1	mRNA	16950051	16950473	16950051	ID=MS.gene08344.t1;Parent=MS.gene08344
chr4.1	exon	16950051	16950473	16950051	ID=MS.gene08344.t1.exon1;Parent=MS.gene08344.t1
chr4.1	CDS	16950051	16950473	16950051	ID=cds.MS.gene08344.t1;Parent=MS.gene08344.t1

Gene Sequence

>MS.gene08344

ATGGGACGAGTCCGAACCAAAACCGTGAAGAAATCCTCTCGTCAAATCATCGAGCGTAACCACCCTAAAATGACACTGGATTTTCACACCAACAAGAAGATCCTTGAAGAAGTCGCTTTGATCCCTTCAAAGCGTCTTCGTAACAAGATTGCTGGTTTCTCTACGCATCTCATGCAGCGTATTCGAAAGGGACCTGTTCGTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGATCGTCGTATGGATTTCGTTCCTGATGTTTCTGCTATTAGGACTGATCATATTGAGGTTGATAAGGAAACCCTAGATATGCTTGCTGCTCTTGGCATGTCGAAGATTCCTGGTGTTGTTCAGGTTAATCGTGTTCTTGTTCAGCAGATCCCATATGGTCGTGGTGCTGGTGCTGGAAGGAGGTTTTAA

Protein sequence

>MS.gene08344.t1

MGRVRTKTVKKSSRQIIERNHPKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMQRIRKGPVRGISLKLQEEERDRRMDFVPDVSAIRTDHIEVDKETLDMLAALGMSKIPGVVQVNRVLVQQIPYGRGAGAGRRF