AlfalfaGEDB | MS.gene034832

M. sativa cultivar XinJiangDaYe / MS.gene034832

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034832.t1	XP_013467927.1	98.4	127	2	0	1	127	1	127	3.60E-60	240.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034832.t1	P49215	90.6	127	10	1	1	125	1	127	2.3e-55	216.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034832.t1	I3SPL5	98.4	127	2	0	1	127	1	127	2.6e-60	240.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene034832	MS.gene023275	PPI
MS.gene034832	MS.gene039073	PPI
MS.gene034832	MS.gene028176	PPI
MS.gene034832	MS.gene039072	PPI
MS.gene034832	MS.gene54136	PPI
MS.gene034832	MS.gene056917	PPI
MS.gene034832	MS.gene34957	PPI
MS.gene034832	MS.gene08343	PPI
MS.gene34958	MS.gene034832	PPI
MS.gene034832	MS.gene08344	PPI
MS.gene034832	MS.gene040536	PPI
MS.gene034832	MS.gene034834	PPI
MS.gene034832	MS.gene023274	PPI
MS.gene034832	MS.gene065715	PPI
MS.gene034834	MS.gene034832	PPI
MS.gene028177	MS.gene034832	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034832.t1	MTR_1g058250	98.425	127	2	0	1	127	1	127	9.07e-89	254

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034832.t1	AT5G04800	87.705	122	15	1	122	1	122	3.64e-76	222
MS.gene034832.t1	AT5G04800	87.705	122	15	1	122	1	122	3.64e-76	222
MS.gene034832.t1	AT5G04800	87.705	122	15	1	122	1	122	3.64e-76	222
MS.gene034832.t1	AT5G04800	87.705	122	15	1	122	1	122	3.64e-76	222
MS.gene034832.t1	AT2G05220	86.885	122	16	1	122	1	122	4.39e-75	219
MS.gene034832.t1	AT2G05220	86.885	122	16	1	122	1	122	4.39e-75	219
MS.gene034832.t1	AT3G10610	86.777	121	16	1	121	1	121	1.73e-74	218
MS.gene034832.t1	AT2G04390	86.066	122	17	1	122	1	122	7.61e-74	216

Find 33 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGAGATTCCCGGGGTTGTTC+AGG	0.315547	1.3:+43121074	MS.gene034832:CDS
TTTGAATCCTCTTCATCAAA+TGG	0.339004	1.3:-43120903	None:intergenic
AGTAGTAACGTTCGATTACT+TGG	0.348773	1.3:-43120780	None:intergenic
TTGTTGCGAAGACGCTTTGA+TGG	0.348895	1.3:-43120862	None:intergenic
CCAAACGGAACCTGCTGAAC+AGG	0.358474	1.3:-43121108	None:intergenic
CTTGATATGCTTGCTGCTCT+TGG	0.359734	1.3:+43121045	MS.gene034832:CDS
CTTGGTATGTCCGAGATTCC+CGG	0.369339	1.3:+43121063	MS.gene034832:CDS
TCTCCTTATCAACCTCAATA+TGG	0.414231	1.3:-43121020	None:intergenic
TTGGTATGTCCGAGATTCCC+GGG	0.419949	1.3:+43121064	MS.gene034832:CDS
GAATCAATGCAACTTCTTCA+AGG	0.430500	1.3:-43120840	None:intergenic
TTGAATCCTCTTCATCAAAT+GGG	0.440060	1.3:-43120902	None:intergenic
TTTGATGAAGAGGATTCAAA+AGG	0.440304	1.3:+43120906	MS.gene034832:CDS
TGAACCTGTTCCTGTTCAGC+AGG	0.453615	1.3:+43121098	MS.gene034832:CDS
GGAAGAGCGTGAGAGGAGGA+TGG	0.458231	1.3:+43120963	MS.gene034832:CDS
TTTGTTGGTGTGGAAATCTA+GGG	0.464737	1.3:-43120812	None:intergenic
GGAACCTGCTGAACAGGAAC+AGG	0.466414	1.3:-43121102	None:intergenic
TGACCATATTGAGGTTGATA+AGG	0.472007	1.3:+43121017	MS.gene034832:CDS
CTGAACAACCCCGGGAATCT+CGG	0.484648	1.3:-43121073	None:intergenic
ATTCAAAAGGGACCGGTTCG+TGG	0.491412	1.3:+43120919	MS.gene034832:CDS
TTTCAACCCATTTGATGAAG+AGG	0.506012	1.3:+43120896	MS.gene034832:CDS
CGTCTTCGCAACAAGATCGC+TGG	0.529414	1.3:+43120871	MS.gene034832:CDS
TTCAACGAGATTCCACGAAC+CGG	0.549035	1.3:-43120931	None:intergenic
TACAAGAGGAAGAGCGTGAG+AGG	0.560260	1.3:+43120956	MS.gene034832:CDS
TTGATGAAGAGGATTCAAAA+GGG	0.572781	1.3:+43120907	MS.gene034832:CDS
GAAGAGGATTCAAAAGGGAC+CGG	0.577199	1.3:+43120912	MS.gene034832:CDS
GTTCTGATAGCAGAAACATC+AGG	0.577897	1.3:-43120994	None:intergenic
TATCAGAACTGACCATATTG+AGG	0.583233	1.3:+43121008	MS.gene034832:CDS
AAGAGGAAGAGCGTGAGAGG+AGG	0.595347	1.3:+43120959	MS.gene034832:CDS
AGGTTCAGCCTGAACAACCC+CGG	0.604387	1.3:-43121082	None:intergenic
GGTTCAGCCTGAACAACCCC+GGG	0.607828	1.3:-43121081	None:intergenic
TGGTATGTCCGAGATTCCCG+GGG	0.623039	1.3:+43121065	MS.gene034832:CDS
ACGTTCGATTACTTGGCGTG+AGG	0.642792	1.3:-43120773	None:intergenic
AATCTCGTTGAAGCTACAAG+AGG	0.693243	1.3:+43120942	MS.gene034832:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTTCAAGGATTTTTTTGT+TGG	-	chr1.3:43120830-43120849	None:intergenic	25.0%
	TTGAATCCTCTTCATCAAAT+GGG	-	chr1.3:43120905-43120924	None:intergenic	30.0%
	TTGATGAAGAGGATTCAAAA+GGG	+	chr1.3:43120907-43120926	MS.gene034832:CDS	30.0%
	TTTGAATCCTCTTCATCAAA+TGG	-	chr1.3:43120906-43120925	None:intergenic	30.0%
	TTTGATGAAGAGGATTCAAA+AGG	+	chr1.3:43120906-43120925	MS.gene034832:CDS	30.0%
	AGTAGTAACGTTCGATTACT+TGG	-	chr1.3:43120783-43120802	None:intergenic	35.0%
	GAATCAATGCAACTTCTTCA+AGG	-	chr1.3:43120843-43120862	None:intergenic	35.0%
	TATCAGAACTGACCATATTG+AGG	+	chr1.3:43121008-43121027	MS.gene034832:CDS	35.0%
	TCTCCTTATCAACCTCAATA+TGG	-	chr1.3:43121023-43121042	None:intergenic	35.0%
	TTTCAACCCATTTGATGAAG+AGG	+	chr1.3:43120896-43120915	MS.gene034832:CDS	35.0%
	TTTGTTGGTGTGGAAATCTA+GGG	-	chr1.3:43120815-43120834	None:intergenic	35.0%
!	TTTTGTTGGTGTGGAAATCT+AGG	-	chr1.3:43120816-43120835	None:intergenic	35.0%
!!	TGACCATATTGAGGTTGATA+AGG	+	chr1.3:43121017-43121036	MS.gene034832:CDS	35.0%
!!!	CAAGGATTTTTTTGTTGGTG+TGG	-	chr1.3:43120825-43120844	None:intergenic	35.0%
!!!	TGAGGATTTTTTCACGGTTT+TGG	-	chr1.3:43120758-43120777	None:intergenic	35.0%
	AATCTCGTTGAAGCTACAAG+AGG	+	chr1.3:43120942-43120961	MS.gene034832:CDS	40.0%
	GTTCTGATAGCAGAAACATC+AGG	-	chr1.3:43120997-43121016	None:intergenic	40.0%
!!!	TTGGCGTGAGGATTTTTTCA+CGG	-	chr1.3:43120764-43120783	None:intergenic	40.0%
	GAAGAGGATTCAAAAGGGAC+CGG	+	chr1.3:43120912-43120931	MS.gene034832:CDS	45.0%
	TTCAACGAGATTCCACGAAC+CGG	-	chr1.3:43120934-43120953	None:intergenic	45.0%
	TTGTTGCGAAGACGCTTTGA+TGG	-	chr1.3:43120865-43120884	None:intergenic	45.0%
!	CTTGATATGCTTGCTGCTCT+TGG	+	chr1.3:43121045-43121064	MS.gene034832:CDS	45.0%
	ACGTTCGATTACTTGGCGTG+AGG	-	chr1.3:43120776-43120795	None:intergenic	50.0%
	CTTGGTATGTCCGAGATTCC+CGG	+	chr1.3:43121063-43121082	MS.gene034832:CDS	50.0%
	TTGGTATGTCCGAGATTCCC+GGG	+	chr1.3:43121064-43121083	MS.gene034832:CDS	50.0%
!	TACAAGAGGAAGAGCGTGAG+AGG	+	chr1.3:43120956-43120975	MS.gene034832:CDS	50.0%
!!	ATTCAAAAGGGACCGGTTCG+TGG	+	chr1.3:43120919-43120938	MS.gene034832:CDS	50.0%
	AGGTTCAGCCTGAACAACCC+CGG	-	chr1.3:43121085-43121104	None:intergenic	55.0%
	CGTCTTCGCAACAAGATCGC+TGG	+	chr1.3:43120871-43120890	MS.gene034832:CDS	55.0%
	CTGAACAACCCCGGGAATCT+CGG	-	chr1.3:43121076-43121095	None:intergenic	55.0%
	TGGTATGTCCGAGATTCCCG+GGG	+	chr1.3:43121065-43121084	MS.gene034832:CDS	55.0%
!	AAGAGGAAGAGCGTGAGAGG+AGG	+	chr1.3:43120959-43120978	MS.gene034832:CDS	55.0%
	CGAGATTCCCGGGGTTGTTC+AGG	+	chr1.3:43121074-43121093	MS.gene034832:CDS	60.0%
	GGTTCAGCCTGAACAACCCC+GGG	-	chr1.3:43121084-43121103	None:intergenic	60.0%
!	GGAAGAGCGTGAGAGGAGGA+TGG	+	chr1.3:43120963-43120982	MS.gene034832:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	43120739	43121119	43120739	ID=MS.gene034832
chr1.3	mRNA	43120739	43121119	43120739	ID=MS.gene034832.t1;Parent=MS.gene034832
chr1.3	exon	43120739	43121119	43120739	ID=MS.gene034832.t1.exon1;Parent=MS.gene034832.t1
chr1.3	CDS	43120739	43121119	43120739	ID=cds.MS.gene034832.t1;Parent=MS.gene034832.t1

Gene Sequence

>MS.gene034832

ATGGGTCGTGTCAGAACCAAAACCGTGAAAAAATCCTCACGCCAAGTAATCGAACGTTACTACTCAAAAATGACCCTAGATTTCCACACCAACAAAAAAATCCTTGAAGAAGTTGCATTGATTCCATCAAAGCGTCTTCGCAACAAGATCGCTGGATTTTCAACCCATTTGATGAAGAGGATTCAAAAGGGACCGGTTCGTGGAATCTCGTTGAAGCTACAAGAGGAAGAGCGTGAGAGGAGGATGGATTTCGTTCCTGATGTTTCTGCTATCAGAACTGACCATATTGAGGTTGATAAGGAGACTCTTGATATGCTTGCTGCTCTTGGTATGTCCGAGATTCCCGGGGTTGTTCAGGCTGAACCTGTTCCTGTTCAGCAG

Protein sequence

>MS.gene034832.t1

MGRVRTKTVKKSSRQVIERYYSKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDHIEVDKETLDMLAALGMSEIPGVVQAEPVPVQQ