AlfalfaGEDB | MS.gene040536

M. sativa cultivar XinJiangDaYe / MS.gene040536

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040536.t1	XP_013467927.1	88.3	145	9	3	1	138	1	144	1.10E-57	232.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040536.t1	P49215	79.9	144	23	1	1	138	1	144	7.3e-55	214.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040536.t1	I3SPL5	88.3	145	9	3	1	138	1	144	7.7e-58	232.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene34957	MS.gene040536	PPI
MS.gene034834	MS.gene040536	PPI
MS.gene028177	MS.gene040536	PPI
MS.gene023275	MS.gene040536	PPI
MS.gene039073	MS.gene040536	PPI
MS.gene54136	MS.gene040536	PPI
MS.gene08344	MS.gene040536	PPI
MS.gene056917	MS.gene040536	PPI
MS.gene08343	MS.gene040536	PPI
MS.gene028176	MS.gene040536	PPI
MS.gene023274	MS.gene040536	PPI
MS.gene039072	MS.gene040536	PPI
MS.gene034832	MS.gene040536	PPI
MS.gene34958	MS.gene040536	PPI
MS.gene065715	MS.gene040536	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040536.t1	MTR_1g058250	96.552	116	4	0	1	116	1	116	2.47e-78	228

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040536.t1	AT5G04800	80.292	137	25	2	1	136	1	136	3.96e-74	218
MS.gene040536.t1	AT5G04800	80.292	137	25	2	1	136	1	136	3.96e-74	218
MS.gene040536.t1	AT5G04800	80.292	137	25	2	1	136	1	136	3.96e-74	218
MS.gene040536.t1	AT5G04800	80.292	137	25	2	1	136	1	136	3.96e-74	218
MS.gene040536.t1	AT2G05220	79.412	136	27	1	1	136	1	135	1.27e-73	216
MS.gene040536.t1	AT2G05220	79.412	136	27	1	1	136	1	135	1.27e-73	216
MS.gene040536.t1	AT2G04390	80.292	137	25	2	1	136	1	136	8.45e-73	214
MS.gene040536.t1	AT3G10610	79.699	133	26	1	1	133	1	132	1.38e-71	211

Find 40 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAACAACAACACCGGGAATC+TGG	0.255977	2.3:+27229415	None:intergenic
AGAGGATTTCTTCACGGTTT+TGG	0.259892	2.3:+27229734	None:intergenic
CTTCTTGTTCGTGTGGAAAT+CGG	0.265901	2.3:+27229674	None:intergenic
GATTCCCGGTGTTGTTGTTC+AGG	0.304413	2.3:-27229412	MS.gene040536:CDS
CTTCCAACACCACCAGTAAC+AGG	0.324593	2.3:+27229366	None:intergenic
CAGCAGCAGATCCCTGTTAC+TGG	0.358701	2.3:-27229378	MS.gene040536:CDS
TTGTTACGAAGACGCTTTGA+TGG	0.364180	2.3:+27229627	None:intergenic
ACAGGGATCTGCTGCTGAAC+AGG	0.364890	2.3:+27229384	None:intergenic
CTTGGTATGTCCCAGATTCC+CGG	0.382777	2.3:-27229426	MS.gene040536:CDS
CTTGACATGCTTGCTGCTCT+TGG	0.389228	2.3:-27229444	MS.gene040536:CDS
CTCAATATAGTCGGTCCTAA+TGG	0.392260	2.3:+27229482	None:intergenic
TTCTTGTTCGTGTGGAAATC+GGG	0.406221	2.3:+27229675	None:intergenic
TTCCTGATGTTTCTGCCATT+AGG	0.411319	2.3:-27229497	MS.gene040536:CDS
TCTCATGAAGCGTATTCAAA+AGG	0.441131	2.3:-27229583	MS.gene040536:CDS
TTCAATGAAATTCCACGAAC+AGG	0.443778	2.3:+27229558	None:intergenic
CGACTATATTGAGGTTGATA+AGG	0.451902	2.3:-27229472	MS.gene040536:CDS
AACAACAACACCGGGAATCT+GGG	0.464609	2.3:+27229416	None:intergenic
GTCCTAATGGCAGAAACATC+AGG	0.475552	2.3:+27229495	None:intergenic
ATCCCTGTTACTGGTGGTGT+TGG	0.478633	2.3:-27229369	MS.gene040536:CDS
CGACGATCTTCTTGTTCGTG+TGG	0.496948	2.3:+27229667	None:intergenic
TTTGGTTCGGACTCTTCCCA+TGG	0.508667	2.3:+27229752	None:intergenic
TTGACGAGAGGATTTCTTCA+CGG	0.510632	2.3:+27229728	None:intergenic
GGAGGAGCGTGAGCGTCGTA+TGG	0.521163	2.3:-27229526	MS.gene040536:CDS
ATTCAAAAGGGACCTGTTCG+TGG	0.521534	2.3:-27229570	MS.gene040536:CDS
CTCATGAAGCGTATTCAAAA+GGG	0.527634	2.3:-27229582	MS.gene040536:CDS
CGTCTTCGTAACAAGATCGC+TGG	0.539364	2.3:-27229618	MS.gene040536:CDS
CATTAGGACCGACTATATTG+AGG	0.545899	2.3:-27229481	MS.gene040536:CDS
CTGTTACTGGTGGTGTTGGA+AGG	0.556457	2.3:-27229365	MS.gene040536:CDS
CAAGAGCAGCAAGCATGTCA+AGG	0.564418	2.3:+27229445	None:intergenic
CTTATCAACCTCAATATAGT+CGG	0.565136	2.3:+27229473	None:intergenic
TTCATTGAAGCTGCAAGAGG+AGG	0.567846	2.3:-27229544	MS.gene040536:CDS
TTACTGGTGGTGTTGGAAGG+AGG	0.588210	2.3:-27229362	MS.gene040536:CDS
AATACGCTTCATGAGATGAG+TGG	0.589822	2.3:+27229590	None:intergenic
ATCAACCTGAACAACAACAC+CGG	0.603304	2.3:+27229407	None:intergenic
CAGCAGATCCCTGTTACTGG+TGG	0.604301	2.3:-27229375	MS.gene040536:CDS
AAGAGCAGCAAGCATGTCAA+GGG	0.613477	2.3:+27229446	None:intergenic
TCAACCTGAACAACAACACC+GGG	0.615929	2.3:+27229408	None:intergenic
TTCCAACACCACCAGTAACA+GGG	0.631505	2.3:+27229367	None:intergenic
AATTTCATTGAAGCTGCAAG+AGG	0.634258	2.3:-27229547	MS.gene040536:CDS
ACGCTCAATCACTTGACGAG+AGG	0.680753	2.3:+27229716	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTATCAACCTCAATATAGT+CGG	+	chr2.3:27229636-27229655	None:intergenic	30.0%
	AATTTCATTGAAGCTGCAAG+AGG	-	chr2.3:27229559-27229578	MS.gene040536:CDS	35.0%
	CTCATGAAGCGTATTCAAAA+GGG	-	chr2.3:27229524-27229543	MS.gene040536:CDS	35.0%
	TCTCATGAAGCGTATTCAAA+AGG	-	chr2.3:27229523-27229542	MS.gene040536:CDS	35.0%
	TTCAATGAAATTCCACGAAC+AGG	+	chr2.3:27229551-27229570	None:intergenic	35.0%
!!	CGACTATATTGAGGTTGATA+AGG	-	chr2.3:27229634-27229653	MS.gene040536:CDS	35.0%
!!!	ATTTCTTCACGGTTTTGGTT+CGG	+	chr2.3:27229370-27229389	None:intergenic	35.0%
	AATACGCTTCATGAGATGAG+TGG	+	chr2.3:27229519-27229538	None:intergenic	40.0%
	ATCAACCTGAACAACAACAC+CGG	+	chr2.3:27229702-27229721	None:intergenic	40.0%
	CATTAGGACCGACTATATTG+AGG	-	chr2.3:27229625-27229644	MS.gene040536:CDS	40.0%
	CTCAATATAGTCGGTCCTAA+TGG	+	chr2.3:27229627-27229646	None:intergenic	40.0%
	CTTCTTGTTCGTGTGGAAAT+CGG	+	chr2.3:27229435-27229454	None:intergenic	40.0%
	TTCTTGTTCGTGTGGAAATC+GGG	+	chr2.3:27229434-27229453	None:intergenic	40.0%
	TTGACGAGAGGATTTCTTCA+CGG	+	chr2.3:27229381-27229400	None:intergenic	40.0%
	TTGTTACGAAGACGCTTTGA+TGG	+	chr2.3:27229482-27229501	None:intergenic	40.0%
!	AGAGGATTTCTTCACGGTTT+TGG	+	chr2.3:27229375-27229394	None:intergenic	40.0%
!	TTCCTGATGTTTCTGCCATT+AGG	-	chr2.3:27229609-27229628	MS.gene040536:CDS	40.0%
	AACAACAACACCGGGAATCT+GGG	+	chr2.3:27229693-27229712	None:intergenic	45.0%
	AAGAGCAGCAAGCATGTCAA+GGG	+	chr2.3:27229663-27229682	None:intergenic	45.0%
	ATTCAAAAGGGACCTGTTCG+TGG	-	chr2.3:27229536-27229555	MS.gene040536:CDS	45.0%
	GTCCTAATGGCAGAAACATC+AGG	+	chr2.3:27229614-27229633	None:intergenic	45.0%
	TCAACCTGAACAACAACACC+GGG	+	chr2.3:27229701-27229720	None:intergenic	45.0%
	TTCATTGAAGCTGCAAGAGG+AGG	-	chr2.3:27229562-27229581	MS.gene040536:CDS	45.0%
	TTCCAACACCACCAGTAACA+GGG	+	chr2.3:27229742-27229761	None:intergenic	45.0%
	ACGCTCAATCACTTGACGAG+AGG	+	chr2.3:27229393-27229412	None:intergenic	50.0%
	CAAGAGCAGCAAGCATGTCA+AGG	+	chr2.3:27229664-27229683	None:intergenic	50.0%
	CGACGATCTTCTTGTTCGTG+TGG	+	chr2.3:27229442-27229461	None:intergenic	50.0%
	CGTCTTCGTAACAAGATCGC+TGG	-	chr2.3:27229488-27229507	MS.gene040536:CDS	50.0%
	CTTCCAACACCACCAGTAAC+AGG	+	chr2.3:27229743-27229762	None:intergenic	50.0%
	CTTGGTATGTCCCAGATTCC+CGG	-	chr2.3:27229680-27229699	MS.gene040536:CDS	50.0%
	GAACAACAACACCGGGAATC+TGG	+	chr2.3:27229694-27229713	None:intergenic	50.0%
!	ATCCCTGTTACTGGTGGTGT+TGG	-	chr2.3:27229737-27229756	MS.gene040536:CDS	50.0%
!	CTGTTACTGGTGGTGTTGGA+AGG	-	chr2.3:27229741-27229760	MS.gene040536:CDS	50.0%
!	CTTGACATGCTTGCTGCTCT+TGG	-	chr2.3:27229662-27229681	MS.gene040536:CDS	50.0%
!	TTACTGGTGGTGTTGGAAGG+AGG	-	chr2.3:27229744-27229763	MS.gene040536:CDS	50.0%
!!	GATTCCCGGTGTTGTTGTTC+AGG	-	chr2.3:27229694-27229713	MS.gene040536:CDS	50.0%
	ACAGGGATCTGCTGCTGAAC+AGG	+	chr2.3:27229725-27229744	None:intergenic	55.0%
	CAGCAGATCCCTGTTACTGG+TGG	-	chr2.3:27229731-27229750	MS.gene040536:CDS	55.0%
	CAGCAGCAGATCCCTGTTAC+TGG	-	chr2.3:27229728-27229747	MS.gene040536:CDS	55.0%
!	GGAGGAGCGTGAGCGTCGTA+TGG	-	chr2.3:27229580-27229599	MS.gene040536:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	27229356	27229772	27229356	ID=MS.gene040536
chr2.3	mRNA	27229356	27229772	27229356	ID=MS.gene040536.t1;Parent=MS.gene040536
chr2.3	exon	27229356	27229772	27229356	ID=MS.gene040536.t1.exon1;Parent=MS.gene040536.t1
chr2.3	CDS	27229356	27229772	27229356	ID=cds.MS.gene040536.t1;Parent=MS.gene040536.t1

Gene Sequence

>MS.gene040536

ATGGGAAGAGTCCGAACCAAAACCGTGAAGAAATCCTCTCGTCAAGTGATTGAGCGTTATTACTCGAAGATGACACCCGATTTCCACACGAACAAGAAGATCGTCGAAGAAGTTGCTTTGATACCATCAAAGCGTCTTCGTAACAAGATCGCTGGTTTCTCCACTCATCTCATGAAGCGTATTCAAAAGGGACCTGTTCGTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGAGCGTCGTATGGATTTCGTTCCTGATGTTTCTGCCATTAGGACCGACTATATTGAGGTTGATAAGGAAACCCTTGACATGCTTGCTGCTCTTGGTATGTCCCAGATTCCCGGTGTTGTTGTTCAGGTTGATCCTGTTCAGCAGCAGATCCCTGTTACTGGTGGTGTTGGAAGGAGGTTTTAA

Protein sequence

>MS.gene040536.t1

MGRVRTKTVKKSSRQVIERYYSKMTPDFHTNKKIVEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDYIEVDKETLDMLAALGMSQIPGVVVQVDPVQQQIPVTGGVGRRF