AlfalfaGEDB | MS.gene028176

M. sativa cultivar XinJiangDaYe / MS.gene028176

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028176.t1	XP_013467927.1	97.2	145	3	1	1	145	1	144	1.80E-68	268.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028176.t1	P49215	82.4	148	19	3	1	145	1	144	7.4e-58	224.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028176.t1	I3SPL5	97.2	145	3	1	1	145	1	144	1.3e-68	268.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene039073	MS.gene028176	PPI
MS.gene028176	MS.gene023275	PPI
MS.gene08344	MS.gene028176	PPI
MS.gene028176	MS.gene056917	PPI
MS.gene056917	MS.gene028176	PPI
MS.gene034832	MS.gene028176	PPI
MS.gene08343	MS.gene028176	PPI
MS.gene028176	MS.gene039072	PPI
MS.gene028176	MS.gene040536	PPI
MS.gene028176	MS.gene54136	PPI
MS.gene028176	MS.gene34957	PPI
MS.gene065715	MS.gene028176	PPI
MS.gene34958	MS.gene028176	PPI
MS.gene028176	MS.gene023274	PPI
MS.gene034834	MS.gene028176	PPI
MS.gene028177	MS.gene028176	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028176.t1	MTR_1g058250	97.241	145	3	1	1	145	1	144	6.01e-100	283

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028176.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene028176.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene028176.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene028176.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene028176.t1	AT2G05220	75.839	149	23	2	1	145	1	140	2.28e-75	221
MS.gene028176.t1	AT2G05220	75.839	149	23	2	1	145	1	140	2.28e-75	221
MS.gene028176.t1	AT2G04390	78.912	147	23	3	1	145	1	141	2.81e-75	221
MS.gene028176.t1	AT3G10610	80.576	139	20	2	1	135	1	136	1.32e-74	219

Find 44 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATGATTTCTTCACGGTTT+TGG	0.256241	4.2:-15992664	None:intergenic
CGAGATTCCCGGTGTTGTTC+AGG	0.275016	4.2:+15992983	MS.gene028176:CDS
GGTGCTGCTGGTGGTGGTGC+TGG	0.304630	4.2:+15993050	MS.gene028176:CDS
CGTCTTCGCAACAAGATTGC+TGG	0.339095	4.2:+15992780	MS.gene028176:CDS
TTCCTGATGTTTCTGCTATT+AGG	0.341443	4.2:+15992901	MS.gene028176:CDS
TTCTTCAAGGATCTTCTTGT+TGG	0.343538	4.2:-15992736	None:intergenic
TTGTTGCGAAGACGCTTTGA+TGG	0.348895	4.2:-15992771	None:intergenic
CTGCTGGTGGTGGTGCTGGA+AGG	0.354715	4.2:+15993054	MS.gene028176:CDS
GGGATCTGCTGAACAGGAAC+AGG	0.359204	4.2:-15993011	None:intergenic
CCAAATGGGATCTGCTGAAC+AGG	0.361093	4.2:-15993017	None:intergenic
CCTGTTCAGCAGATCCCATT+TGG	0.377310	4.2:+15993017	MS.gene028176:CDS
CTTGGTATGTCCGAGATTCC+CGG	0.385581	4.2:+15992972	MS.gene028176:CDS
GGTCGTGGTGCTGGTGCTGC+TGG	0.397665	4.2:+15993038	MS.gene028176:CDS
ATCCCATTTGGTCGTGGTGC+TGG	0.404490	4.2:+15993029	MS.gene028176:CDS
CCTTCTGAATACGTTTCATA+AGG	0.423532	4.2:-15992815	None:intergenic
CTAGACATGCTTGCTGCTCT+TGG	0.432500	4.2:+15992954	MS.gene028176:CDS
GCACCAGCACCACGACCAAA+TGG	0.436244	4.2:-15993032	None:intergenic
TTCAATGAAATTCCACGAAC+AGG	0.443778	4.2:-15992840	None:intergenic
GGTGCTGGTGCTGCTGGTGG+TGG	0.444507	4.2:+15993044	MS.gene028176:CDS
CTGAACAACACCGGGAATCT+CGG	0.461504	4.2:-15992982	None:intergenic
GAATCAAAGCAACTTCTTCA+AGG	0.471503	4.2:-15992749	None:intergenic
CAAGAGCAGCAAGCATGTCT+AGG	0.477650	4.2:-15992953	None:intergenic
TGATCATATTGAGGTTGATA+AGG	0.481558	4.2:+15992926	MS.gene028176:CDS
CAAGGATCTTCTTGTTGGTG+TGG	0.483634	4.2:-15992731	None:intergenic
TTGACGAGATGATTTCTTCA+CGG	0.484691	4.2:-15992670	None:intergenic
CTGGTGGTGGTGCTGGAAGG+AGG	0.484753	4.2:+15993057	MS.gene028176:CDS
GTCCTAATAGCAGAAACATC+AGG	0.505221	4.2:-15992903	None:intergenic
GGAGGAGCGTGAGCGTCGTA+TGG	0.521163	4.2:+15992872	MS.gene028176:CDS
TTTGGTTCGGACTCGTCCCA+TGG	0.523667	4.2:-15992646	None:intergenic
CGTGGTGCTGGTGCTGCTGG+TGG	0.525996	4.2:+15993041	MS.gene028176:CDS
ATTCAGAAGGGACCTGTTCG+TGG	0.526647	4.2:+15992828	MS.gene028176:CDS
CACCAGCACCACGACCAAAT+GGG	0.535186	4.2:-15993031	None:intergenic
CAGCAGATCCCATTTGGTCG+TGG	0.539254	4.2:+15993023	MS.gene028176:CDS
GTGGAAATCGAGTGTCATCT+TGG	0.542107	4.2:-15992712	None:intergenic
TATTAGGACTGATCATATTG+AGG	0.542204	4.2:+15992917	MS.gene028176:CDS
AAGAGCAGCAAGCATGTCTA+GGG	0.560169	4.2:-15992952	None:intergenic
TTCATTGAAGCTGCAAGAGG+AGG	0.567846	4.2:+15992854	MS.gene028176:CDS
CCTTATGAAACGTATTCAGA+AGG	0.588661	4.2:+15992815	MS.gene028176:CDS
AGGGTCAACCTGAACAACAC+CGG	0.593915	4.2:-15992991	None:intergenic
GGGTCAACCTGAACAACACC+GGG	0.608103	4.2:-15992990	None:intergenic
AATACGTTTCATAAGGTGAG+TGG	0.609703	4.2:-15992808	None:intergenic
AATTTCATTGAAGCTGCAAG+AGG	0.634258	4.2:+15992851	MS.gene028176:CDS
CTTATGAAACGTATTCAGAA+GGG	0.635445	4.2:+15992816	MS.gene028176:CDS
GGATCTGCTGAACAGGAACA+GGG	0.673645	4.2:-15993010	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTATGAAACGTATTCAGAA+GGG	+	chr4.2:15992816-15992835	MS.gene028176:CDS	30.0%
	TATTAGGACTGATCATATTG+AGG	+	chr4.2:15992917-15992936	MS.gene028176:CDS	30.0%
!!	TGATCATATTGAGGTTGATA+AGG	+	chr4.2:15992926-15992945	MS.gene028176:CDS	30.0%
	AATACGTTTCATAAGGTGAG+TGG	-	chr4.2:15992811-15992830	None:intergenic	35.0%
	AATTTCATTGAAGCTGCAAG+AGG	+	chr4.2:15992851-15992870	MS.gene028176:CDS	35.0%
	CCTTATGAAACGTATTCAGA+AGG	+	chr4.2:15992815-15992834	MS.gene028176:CDS	35.0%
	CCTTCTGAATACGTTTCATA+AGG	-	chr4.2:15992818-15992837	None:intergenic	35.0%
	GAATCAAAGCAACTTCTTCA+AGG	-	chr4.2:15992752-15992771	None:intergenic	35.0%
	TTCAATGAAATTCCACGAAC+AGG	-	chr4.2:15992843-15992862	None:intergenic	35.0%
	TTCTTCAAGGATCTTCTTGT+TGG	-	chr4.2:15992739-15992758	None:intergenic	35.0%
	TTGACGAGATGATTTCTTCA+CGG	-	chr4.2:15992673-15992692	None:intergenic	35.0%
!	AGATGATTTCTTCACGGTTT+TGG	-	chr4.2:15992667-15992686	None:intergenic	35.0%
!	TTCCTGATGTTTCTGCTATT+AGG	+	chr4.2:15992901-15992920	MS.gene028176:CDS	35.0%
!!!	ATTTCTTCACGGTTTTGGTT+CGG	-	chr4.2:15992662-15992681	None:intergenic	35.0%
	GTCCTAATAGCAGAAACATC+AGG	-	chr4.2:15992906-15992925	None:intergenic	40.0%
	AAGAGCAGCAAGCATGTCTA+GGG	-	chr4.2:15992955-15992974	None:intergenic	45.0%
	CAAGGATCTTCTTGTTGGTG+TGG	-	chr4.2:15992734-15992753	None:intergenic	45.0%
	GTGGAAATCGAGTGTCATCT+TGG	-	chr4.2:15992715-15992734	None:intergenic	45.0%
	TTCATTGAAGCTGCAAGAGG+AGG	+	chr4.2:15992854-15992873	MS.gene028176:CDS	45.0%
	TTGTTGCGAAGACGCTTTGA+TGG	-	chr4.2:15992774-15992793	None:intergenic	45.0%
	AGGGTCAACCTGAACAACAC+CGG	-	chr4.2:15992994-15993013	None:intergenic	50.0%
	ATTCAGAAGGGACCTGTTCG+TGG	+	chr4.2:15992828-15992847	MS.gene028176:CDS	50.0%
	CAAGAGCAGCAAGCATGTCT+AGG	-	chr4.2:15992956-15992975	None:intergenic	50.0%
	CCAAATGGGATCTGCTGAAC+AGG	-	chr4.2:15993020-15993039	None:intergenic	50.0%
	CCTGTTCAGCAGATCCCATT+TGG	+	chr4.2:15993017-15993036	MS.gene028176:CDS	50.0%
	CGTCTTCGCAACAAGATTGC+TGG	+	chr4.2:15992780-15992799	MS.gene028176:CDS	50.0%
	CTGAACAACACCGGGAATCT+CGG	-	chr4.2:15992985-15993004	None:intergenic	50.0%
	CTTGGTATGTCCGAGATTCC+CGG	+	chr4.2:15992972-15992991	MS.gene028176:CDS	50.0%
	GGATCTGCTGAACAGGAACA+GGG	-	chr4.2:15993013-15993032	None:intergenic	50.0%
!	CTAGACATGCTTGCTGCTCT+TGG	+	chr4.2:15992954-15992973	MS.gene028176:CDS	50.0%
	ATCCCATTTGGTCGTGGTGC+TGG	+	chr4.2:15993029-15993048	MS.gene028176:CDS	55.0%
	CACCAGCACCACGACCAAAT+GGG	-	chr4.2:15993034-15993053	None:intergenic	55.0%
	CAGCAGATCCCATTTGGTCG+TGG	+	chr4.2:15993023-15993042	MS.gene028176:CDS	55.0%
	GGGATCTGCTGAACAGGAAC+AGG	-	chr4.2:15993014-15993033	None:intergenic	55.0%
	GGGTCAACCTGAACAACACC+GGG	-	chr4.2:15992993-15993012	None:intergenic	55.0%
!!	CGAGATTCCCGGTGTTGTTC+AGG	+	chr4.2:15992983-15993002	MS.gene028176:CDS	55.0%
	GCACCAGCACCACGACCAAA+TGG	-	chr4.2:15993035-15993054	None:intergenic	60.0%
!	GGAGGAGCGTGAGCGTCGTA+TGG	+	chr4.2:15992872-15992891	MS.gene028176:CDS	65.0%
!!	CTGCTGGTGGTGGTGCTGGA+AGG	+	chr4.2:15993054-15993073	MS.gene028176:CDS	65.0%
!!	CTGGTGGTGGTGCTGGAAGG+AGG	+	chr4.2:15993057-15993076	MS.gene028176:CDS	65.0%
!!	CGTGGTGCTGGTGCTGCTGG+TGG	+	chr4.2:15993041-15993060	MS.gene028176:CDS	70.0%
!!	GGTCGTGGTGCTGGTGCTGC+TGG	+	chr4.2:15993038-15993057	MS.gene028176:CDS	70.0%
!!	GGTGCTGCTGGTGGTGGTGC+TGG	+	chr4.2:15993050-15993069	MS.gene028176:CDS	70.0%
!!	GGTGCTGGTGCTGCTGGTGG+TGG	+	chr4.2:15993044-15993063	MS.gene028176:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	15992648	15993085	15992648	ID=MS.gene028176
chr4.2	mRNA	15992648	15993085	15992648	ID=MS.gene028176.t1;Parent=MS.gene028176
chr4.2	exon	15992648	15993085	15992648	ID=MS.gene028176.t1.exon1;Parent=MS.gene028176.t1
chr4.2	CDS	15992648	15993085	15992648	ID=cds.MS.gene028176.t1;Parent=MS.gene028176.t1

Gene Sequence

>MS.gene028176

ATGGGACGAGTCCGAACCAAAACCGTGAAGAAATCATCTCGTCAAGTCATTGAGCGTTATTACTCCAAGATGACACTCGATTTCCACACCAACAAGAAGATCCTTGAAGAAGTTGCTTTGATTCCATCAAAGCGTCTTCGCAACAAGATTGCTGGTTTTTCCACTCACCTTATGAAACGTATTCAGAAGGGACCTGTTCGTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGAGCGTCGTATGGACTTCGTTCCTGATGTTTCTGCTATTAGGACTGATCATATTGAGGTTGATAAGGAAACCCTAGACATGCTTGCTGCTCTTGGTATGTCCGAGATTCCCGGTGTTGTTCAGGTTGACCCTGTTCCTGTTCAGCAGATCCCATTTGGTCGTGGTGCTGGTGCTGCTGGTGGTGGTGCTGGAAGGAGGTTTTAA

Protein sequence

>MS.gene028176.t1

MGRVRTKTVKKSSRQVIERYYSKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDHIEVDKETLDMLAALGMSEIPGVVQVDPVPVQQIPFGRGAGAAGGGAGRRF