AlfalfaGEDB | MS.gene54136

M. sativa cultivar XinJiangDaYe / MS.gene54136

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54136.t1	XP_013467927.1	88.3	145	9	3	1	138	1	144	1.10E-57	232.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54136.t1	P49215	79.9	144	23	1	1	138	1	144	7.3e-55	214.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54136.t1	I3SPL5	88.3	145	9	3	1	138	1	144	7.7e-58	232.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene34957	MS.gene54136	PPI
MS.gene034834	MS.gene54136	PPI
MS.gene028177	MS.gene54136	PPI
MS.gene023275	MS.gene54136	PPI
MS.gene54136	MS.gene040536	PPI
MS.gene039073	MS.gene54136	PPI
MS.gene08344	MS.gene54136	PPI
MS.gene54136	MS.gene023274	PPI
MS.gene056917	MS.gene54136	PPI
MS.gene034832	MS.gene54136	PPI
MS.gene08343	MS.gene54136	PPI
MS.gene028176	MS.gene54136	PPI
MS.gene039072	MS.gene54136	PPI
MS.gene065715	MS.gene54136	PPI
MS.gene34958	MS.gene54136	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54136.t1	MTR_1g058250	96.552	116	4	0	1	116	1	116	2.47e-78	228

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54136.t1	AT5G04800	80.292	137	25	2	1	136	1	136	3.96e-74	218
MS.gene54136.t1	AT5G04800	80.292	137	25	2	1	136	1	136	3.96e-74	218
MS.gene54136.t1	AT5G04800	80.292	137	25	2	1	136	1	136	3.96e-74	218
MS.gene54136.t1	AT5G04800	80.292	137	25	2	1	136	1	136	3.96e-74	218
MS.gene54136.t1	AT2G05220	79.412	136	27	1	1	136	1	135	1.27e-73	216
MS.gene54136.t1	AT2G05220	79.412	136	27	1	1	136	1	135	1.27e-73	216
MS.gene54136.t1	AT2G04390	80.292	137	25	2	1	136	1	136	8.45e-73	214
MS.gene54136.t1	AT3G10610	79.699	133	26	1	1	133	1	132	1.38e-71	211

Find 40 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAACAACAACACCGGGAATC+TGG	0.255977	2.2:+23406029	None:intergenic
AGAGGATTTCTTCACGGTTT+TGG	0.259892	2.2:+23406348	None:intergenic
CTTCTTGTTCGTGTGGAAAT+CGG	0.265901	2.2:+23406288	None:intergenic
GATTCCCGGTGTTGTTGTTC+AGG	0.304413	2.2:-23406026	MS.gene54136:CDS
CTTCCAACACCACCAGTAAC+AGG	0.324593	2.2:+23405980	None:intergenic
CAGCAGCAGATCCCTGTTAC+TGG	0.358701	2.2:-23405992	MS.gene54136:CDS
TTGTTACGAAGACGCTTTGA+TGG	0.364180	2.2:+23406241	None:intergenic
ACAGGGATCTGCTGCTGAAC+AGG	0.364890	2.2:+23405998	None:intergenic
CTTGGTATGTCCCAGATTCC+CGG	0.382777	2.2:-23406040	MS.gene54136:CDS
CTTGACATGCTTGCTGCTCT+TGG	0.389228	2.2:-23406058	MS.gene54136:CDS
CTCAATATAGTCGGTCCTAA+TGG	0.392260	2.2:+23406096	None:intergenic
TTCTTGTTCGTGTGGAAATC+GGG	0.406221	2.2:+23406289	None:intergenic
TTCCTGATGTTTCTGCCATT+AGG	0.411319	2.2:-23406111	MS.gene54136:CDS
TCTCATGAAGCGTATTCAAA+AGG	0.441131	2.2:-23406197	MS.gene54136:CDS
TTCAATGAAATTCCACGAAC+AGG	0.443778	2.2:+23406172	None:intergenic
CGACTATATTGAGGTTGATA+AGG	0.451902	2.2:-23406086	MS.gene54136:CDS
AACAACAACACCGGGAATCT+GGG	0.464609	2.2:+23406030	None:intergenic
GTCCTAATGGCAGAAACATC+AGG	0.475552	2.2:+23406109	None:intergenic
ATCCCTGTTACTGGTGGTGT+TGG	0.478633	2.2:-23405983	MS.gene54136:CDS
CGACGATCTTCTTGTTCGTG+TGG	0.496948	2.2:+23406281	None:intergenic
TTTGGTTCGGACTCTTCCCA+TGG	0.508667	2.2:+23406366	None:intergenic
TTGACGAGAGGATTTCTTCA+CGG	0.510632	2.2:+23406342	None:intergenic
GGAGGAGCGTGAGCGTCGTA+TGG	0.521163	2.2:-23406140	MS.gene54136:CDS
ATTCAAAAGGGACCTGTTCG+TGG	0.521534	2.2:-23406184	MS.gene54136:CDS
CTCATGAAGCGTATTCAAAA+GGG	0.527634	2.2:-23406196	MS.gene54136:CDS
CGTCTTCGTAACAAGATCGC+TGG	0.539364	2.2:-23406232	MS.gene54136:CDS
CATTAGGACCGACTATATTG+AGG	0.545899	2.2:-23406095	MS.gene54136:CDS
CTGTTACTGGTGGTGTTGGA+AGG	0.556457	2.2:-23405979	MS.gene54136:CDS
CAAGAGCAGCAAGCATGTCA+AGG	0.564418	2.2:+23406059	None:intergenic
CTTATCAACCTCAATATAGT+CGG	0.565136	2.2:+23406087	None:intergenic
TTCATTGAAGCTGCAAGAGG+AGG	0.567846	2.2:-23406158	MS.gene54136:CDS
TTACTGGTGGTGTTGGAAGG+AGG	0.588210	2.2:-23405976	MS.gene54136:CDS
AATACGCTTCATGAGATGAG+TGG	0.589822	2.2:+23406204	None:intergenic
ATCAACCTGAACAACAACAC+CGG	0.603304	2.2:+23406021	None:intergenic
CAGCAGATCCCTGTTACTGG+TGG	0.604301	2.2:-23405989	MS.gene54136:CDS
AAGAGCAGCAAGCATGTCAA+GGG	0.613477	2.2:+23406060	None:intergenic
TCAACCTGAACAACAACACC+GGG	0.615929	2.2:+23406022	None:intergenic
TTCCAACACCACCAGTAACA+GGG	0.631505	2.2:+23405981	None:intergenic
AATTTCATTGAAGCTGCAAG+AGG	0.634258	2.2:-23406161	MS.gene54136:CDS
ACGCTCAATCACTTGACGAG+AGG	0.680753	2.2:+23406330	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTATCAACCTCAATATAGT+CGG	+	chr2.2:23406250-23406269	None:intergenic	30.0%
	AATTTCATTGAAGCTGCAAG+AGG	-	chr2.2:23406173-23406192	MS.gene54136:CDS	35.0%
	CTCATGAAGCGTATTCAAAA+GGG	-	chr2.2:23406138-23406157	MS.gene54136:CDS	35.0%
	TCTCATGAAGCGTATTCAAA+AGG	-	chr2.2:23406137-23406156	MS.gene54136:CDS	35.0%
	TTCAATGAAATTCCACGAAC+AGG	+	chr2.2:23406165-23406184	None:intergenic	35.0%
!!	CGACTATATTGAGGTTGATA+AGG	-	chr2.2:23406248-23406267	MS.gene54136:CDS	35.0%
!!!	ATTTCTTCACGGTTTTGGTT+CGG	+	chr2.2:23405984-23406003	None:intergenic	35.0%
	AATACGCTTCATGAGATGAG+TGG	+	chr2.2:23406133-23406152	None:intergenic	40.0%
	ATCAACCTGAACAACAACAC+CGG	+	chr2.2:23406316-23406335	None:intergenic	40.0%
	CATTAGGACCGACTATATTG+AGG	-	chr2.2:23406239-23406258	MS.gene54136:CDS	40.0%
	CTCAATATAGTCGGTCCTAA+TGG	+	chr2.2:23406241-23406260	None:intergenic	40.0%
	CTTCTTGTTCGTGTGGAAAT+CGG	+	chr2.2:23406049-23406068	None:intergenic	40.0%
	TTCTTGTTCGTGTGGAAATC+GGG	+	chr2.2:23406048-23406067	None:intergenic	40.0%
	TTGACGAGAGGATTTCTTCA+CGG	+	chr2.2:23405995-23406014	None:intergenic	40.0%
	TTGTTACGAAGACGCTTTGA+TGG	+	chr2.2:23406096-23406115	None:intergenic	40.0%
!	AGAGGATTTCTTCACGGTTT+TGG	+	chr2.2:23405989-23406008	None:intergenic	40.0%
!	TTCCTGATGTTTCTGCCATT+AGG	-	chr2.2:23406223-23406242	MS.gene54136:CDS	40.0%
	AACAACAACACCGGGAATCT+GGG	+	chr2.2:23406307-23406326	None:intergenic	45.0%
	AAGAGCAGCAAGCATGTCAA+GGG	+	chr2.2:23406277-23406296	None:intergenic	45.0%
	ATTCAAAAGGGACCTGTTCG+TGG	-	chr2.2:23406150-23406169	MS.gene54136:CDS	45.0%
	GTCCTAATGGCAGAAACATC+AGG	+	chr2.2:23406228-23406247	None:intergenic	45.0%
	TCAACCTGAACAACAACACC+GGG	+	chr2.2:23406315-23406334	None:intergenic	45.0%
	TTCATTGAAGCTGCAAGAGG+AGG	-	chr2.2:23406176-23406195	MS.gene54136:CDS	45.0%
	TTCCAACACCACCAGTAACA+GGG	+	chr2.2:23406356-23406375	None:intergenic	45.0%
	ACGCTCAATCACTTGACGAG+AGG	+	chr2.2:23406007-23406026	None:intergenic	50.0%
	CAAGAGCAGCAAGCATGTCA+AGG	+	chr2.2:23406278-23406297	None:intergenic	50.0%
	CGACGATCTTCTTGTTCGTG+TGG	+	chr2.2:23406056-23406075	None:intergenic	50.0%
	CGTCTTCGTAACAAGATCGC+TGG	-	chr2.2:23406102-23406121	MS.gene54136:CDS	50.0%
	CTTCCAACACCACCAGTAAC+AGG	+	chr2.2:23406357-23406376	None:intergenic	50.0%
	CTTGGTATGTCCCAGATTCC+CGG	-	chr2.2:23406294-23406313	MS.gene54136:CDS	50.0%
	GAACAACAACACCGGGAATC+TGG	+	chr2.2:23406308-23406327	None:intergenic	50.0%
!	ATCCCTGTTACTGGTGGTGT+TGG	-	chr2.2:23406351-23406370	MS.gene54136:CDS	50.0%
!	CTGTTACTGGTGGTGTTGGA+AGG	-	chr2.2:23406355-23406374	MS.gene54136:CDS	50.0%
!	CTTGACATGCTTGCTGCTCT+TGG	-	chr2.2:23406276-23406295	MS.gene54136:CDS	50.0%
!	TTACTGGTGGTGTTGGAAGG+AGG	-	chr2.2:23406358-23406377	MS.gene54136:CDS	50.0%
!!	GATTCCCGGTGTTGTTGTTC+AGG	-	chr2.2:23406308-23406327	MS.gene54136:CDS	50.0%
	ACAGGGATCTGCTGCTGAAC+AGG	+	chr2.2:23406339-23406358	None:intergenic	55.0%
	CAGCAGATCCCTGTTACTGG+TGG	-	chr2.2:23406345-23406364	MS.gene54136:CDS	55.0%
	CAGCAGCAGATCCCTGTTAC+TGG	-	chr2.2:23406342-23406361	MS.gene54136:CDS	55.0%
!	GGAGGAGCGTGAGCGTCGTA+TGG	-	chr2.2:23406194-23406213	MS.gene54136:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	23405970	23406386	23405970	ID=MS.gene54136
chr2.2	mRNA	23405970	23406386	23405970	ID=MS.gene54136.t1;Parent=MS.gene54136
chr2.2	exon	23405970	23406386	23405970	ID=MS.gene54136.t1.exon1;Parent=MS.gene54136.t1
chr2.2	CDS	23405970	23406386	23405970	ID=cds.MS.gene54136.t1;Parent=MS.gene54136.t1

Gene Sequence

>MS.gene54136

ATGGGAAGAGTCCGAACCAAAACCGTGAAGAAATCCTCTCGTCAAGTGATTGAGCGTTATTACTCGAAGATGACACCCGATTTCCACACGAACAAGAAGATCGTCGAAGAAGTTGCTTTGATACCATCAAAGCGTCTTCGTAACAAGATCGCTGGTTTCTCCACTCATCTCATGAAGCGTATTCAAAAGGGACCTGTTCGTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGAGCGTCGTATGGATTTCGTTCCTGATGTTTCTGCCATTAGGACCGACTATATTGAGGTTGATAAGGAAACCCTTGACATGCTTGCTGCTCTTGGTATGTCCCAGATTCCCGGTGTTGTTGTTCAGGTTGATCCTGTTCAGCAGCAGATCCCTGTTACTGGTGGTGTTGGAAGGAGGTTTTAA

Protein sequence

>MS.gene54136.t1

MGRVRTKTVKKSSRQVIERYYSKMTPDFHTNKKIVEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDYIEVDKETLDMLAALGMSQIPGVVVQVDPVQQQIPVTGGVGRRF