AlfalfaGEDB | MS.gene039073

M. sativa cultivar XinJiangDaYe / MS.gene039073

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039073.t1	XP_013467927.1	88.3	145	8	3	1	137	1	144	8.10E-58	233

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039073.t1	P49215	79.9	144	22	1	1	137	1	144	1.9e-55	216.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039073.t1	I3SPL5	88.3	145	8	3	1	137	1	144	5.9e-58	233.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene039073	MS.gene34957	PPI
MS.gene08344	MS.gene039073	PPI
MS.gene039073	MS.gene028176	PPI
MS.gene039073	MS.gene040536	PPI
MS.gene039073	MS.gene54136	PPI
MS.gene034832	MS.gene039073	PPI
MS.gene08343	MS.gene039073	PPI
MS.gene039073	MS.gene023274	PPI
MS.gene065715	MS.gene039073	PPI
MS.gene34958	MS.gene039073	PPI
MS.gene039073	MS.gene039072	PPI
MS.gene034834	MS.gene039073	PPI
MS.gene028177	MS.gene039073	PPI
MS.gene039073	MS.gene023275	PPI
MS.gene039073	MS.gene056917	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039073.t1	MTR_1g058250	96.552	116	4	0	1	116	1	116	3.18e-78	228

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene039073.t1	AT5G04800	79.851	134	26	1	1	134	1	133	7.04e-73	214
MS.gene039073.t1	AT5G04800	79.851	134	26	1	1	134	1	133	7.04e-73	214
MS.gene039073.t1	AT5G04800	79.851	134	26	1	1	134	1	133	7.04e-73	214
MS.gene039073.t1	AT5G04800	79.851	134	26	1	1	134	1	133	7.04e-73	214
MS.gene039073.t1	AT2G04390	81.481	135	21	2	1	132	1	134	1.20e-72	214
MS.gene039073.t1	AT2G05220	78.676	136	28	1	1	136	1	135	2.96e-72	213
MS.gene039073.t1	AT2G05220	78.676	136	28	1	1	136	1	135	2.96e-72	213
MS.gene039073.t1	AT3G10610	86.325	117	16	0	1	117	1	117	1.67e-70	208

Find 39 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAACAACAACACCGGGAATC+TGG	0.255977	2.4:+26670752	None:intergenic
CTTCTTGTTCGTGTGGAAAT+CGG	0.265901	2.4:+26671011	None:intergenic
GTTCAGCAGATCCCTGTTAC+TGG	0.300071	2.4:-26670718	MS.gene039073:CDS
GATTCCCGGTGTTGTTGTTC+AGG	0.304413	2.4:-26670749	MS.gene039073:CDS
CTTCCAACACCACCAGTAAC+AGG	0.324593	2.4:+26670706	None:intergenic
TTGTTACGAAGACGCTTTGA+TGG	0.364180	2.4:+26670964	None:intergenic
CTTGGTATGTCCCAGATTCC+CGG	0.382777	2.4:-26670763	MS.gene039073:CDS
CTTGACATGCTTGCTGCTCT+TGG	0.389228	2.4:-26670781	MS.gene039073:CDS
CTCAATATAGTCGGTCCTAA+TGG	0.392260	2.4:+26670819	None:intergenic
TTCTTGTTCGTGTGGAAATC+GGG	0.406221	2.4:+26671012	None:intergenic
GTAACAGGGATCTGCTGAAC+AGG	0.408312	2.4:+26670721	None:intergenic
TTCCTGATGTTTCTGCCATT+AGG	0.411319	2.4:-26670834	MS.gene039073:CDS
TCTCATGAAGCGTATTCAAA+AGG	0.441131	2.4:-26670920	MS.gene039073:CDS
TTCAATGAAATTCCACGAAC+AGG	0.443778	2.4:+26670895	None:intergenic
CGACTATATTGAGGTTGATA+AGG	0.451902	2.4:-26670809	MS.gene039073:CDS
AACAACAACACCGGGAATCT+GGG	0.464609	2.4:+26670753	None:intergenic
GTCCTAATGGCAGAAACATC+AGG	0.475552	2.4:+26670832	None:intergenic
ATCCCTGTTACTGGTGGTGT+TGG	0.478633	2.4:-26670709	MS.gene039073:CDS
CGACGATCTTCTTGTTCGTG+TGG	0.496948	2.4:+26671004	None:intergenic
TTGACGAGAGGATTTCTTCA+CGG	0.510632	2.4:+26671065	None:intergenic
TTTCGTTCGGACTCGTCCCA+TGG	0.514391	2.4:+26671089	None:intergenic
GGAGGAGCGTGAGCGTCGTA+TGG	0.521163	2.4:-26670863	MS.gene039073:CDS
ATTCAAAAGGGACCTGTTCG+TGG	0.521534	2.4:-26670907	MS.gene039073:CDS
CTCATGAAGCGTATTCAAAA+GGG	0.527634	2.4:-26670919	MS.gene039073:CDS
CGTCTTCGTAACAAGATCGC+TGG	0.539364	2.4:-26670955	MS.gene039073:CDS
CATTAGGACCGACTATATTG+AGG	0.545899	2.4:-26670818	MS.gene039073:CDS
CTGTTACTGGTGGTGTTGGA+AGG	0.556457	2.4:-26670705	MS.gene039073:CDS
CAAGAGCAGCAAGCATGTCA+AGG	0.564418	2.4:+26670782	None:intergenic
CTTATCAACCTCAATATAGT+CGG	0.565136	2.4:+26670810	None:intergenic
TTCATTGAAGCTGCAAGAGG+AGG	0.567846	2.4:-26670881	MS.gene039073:CDS
TTACTGGTGGTGTTGGAAGG+AGG	0.588210	2.4:-26670702	MS.gene039073:CDS
AATACGCTTCATGAGATGAG+TGG	0.589822	2.4:+26670927	None:intergenic
ATCAACCTGAACAACAACAC+CGG	0.603304	2.4:+26670744	None:intergenic
AAGAGCAGCAAGCATGTCAA+GGG	0.613477	2.4:+26670783	None:intergenic
TCAACCTGAACAACAACACC+GGG	0.615929	2.4:+26670745	None:intergenic
TTCCAACACCACCAGTAACA+GGG	0.631505	2.4:+26670707	None:intergenic
AATTTCATTGAAGCTGCAAG+AGG	0.634258	2.4:-26670884	MS.gene039073:CDS
CAGCAGATCCCTGTTACTGG+TGG	0.650302	2.4:-26670715	MS.gene039073:CDS
ACGCTCAATCACTTGACGAG+AGG	0.680753	2.4:+26671053	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTATCAACCTCAATATAGT+CGG	+	chr2.4:26670976-26670995	None:intergenic	30.0%
	AATTTCATTGAAGCTGCAAG+AGG	-	chr2.4:26670899-26670918	MS.gene039073:CDS	35.0%
	CTCATGAAGCGTATTCAAAA+GGG	-	chr2.4:26670864-26670883	MS.gene039073:CDS	35.0%
	TCTCATGAAGCGTATTCAAA+AGG	-	chr2.4:26670863-26670882	MS.gene039073:CDS	35.0%
	TTCAATGAAATTCCACGAAC+AGG	+	chr2.4:26670891-26670910	None:intergenic	35.0%
!	ATTTCTTCACGGTTTTCGTT+CGG	+	chr2.4:26670710-26670729	None:intergenic	35.0%
!!	CGACTATATTGAGGTTGATA+AGG	-	chr2.4:26670974-26670993	MS.gene039073:CDS	35.0%
	AATACGCTTCATGAGATGAG+TGG	+	chr2.4:26670859-26670878	None:intergenic	40.0%
	ATCAACCTGAACAACAACAC+CGG	+	chr2.4:26671042-26671061	None:intergenic	40.0%
	CATTAGGACCGACTATATTG+AGG	-	chr2.4:26670965-26670984	MS.gene039073:CDS	40.0%
	CTCAATATAGTCGGTCCTAA+TGG	+	chr2.4:26670967-26670986	None:intergenic	40.0%
	CTTCTTGTTCGTGTGGAAAT+CGG	+	chr2.4:26670775-26670794	None:intergenic	40.0%
	TTCTTGTTCGTGTGGAAATC+GGG	+	chr2.4:26670774-26670793	None:intergenic	40.0%
	TTGACGAGAGGATTTCTTCA+CGG	+	chr2.4:26670721-26670740	None:intergenic	40.0%
	TTGTTACGAAGACGCTTTGA+TGG	+	chr2.4:26670822-26670841	None:intergenic	40.0%
!	TTCCTGATGTTTCTGCCATT+AGG	-	chr2.4:26670949-26670968	MS.gene039073:CDS	40.0%
	AACAACAACACCGGGAATCT+GGG	+	chr2.4:26671033-26671052	None:intergenic	45.0%
	AAGAGCAGCAAGCATGTCAA+GGG	+	chr2.4:26671003-26671022	None:intergenic	45.0%
	ATTCAAAAGGGACCTGTTCG+TGG	-	chr2.4:26670876-26670895	MS.gene039073:CDS	45.0%
	GTCCTAATGGCAGAAACATC+AGG	+	chr2.4:26670954-26670973	None:intergenic	45.0%
	TCAACCTGAACAACAACACC+GGG	+	chr2.4:26671041-26671060	None:intergenic	45.0%
	TTCATTGAAGCTGCAAGAGG+AGG	-	chr2.4:26670902-26670921	MS.gene039073:CDS	45.0%
	TTCCAACACCACCAGTAACA+GGG	+	chr2.4:26671079-26671098	None:intergenic	45.0%
	ACGCTCAATCACTTGACGAG+AGG	+	chr2.4:26670733-26670752	None:intergenic	50.0%
	CAAGAGCAGCAAGCATGTCA+AGG	+	chr2.4:26671004-26671023	None:intergenic	50.0%
	CGACGATCTTCTTGTTCGTG+TGG	+	chr2.4:26670782-26670801	None:intergenic	50.0%
	CGTCTTCGTAACAAGATCGC+TGG	-	chr2.4:26670828-26670847	MS.gene039073:CDS	50.0%
	CTTCCAACACCACCAGTAAC+AGG	+	chr2.4:26671080-26671099	None:intergenic	50.0%
	CTTGGTATGTCCCAGATTCC+CGG	-	chr2.4:26671020-26671039	MS.gene039073:CDS	50.0%
	GAACAACAACACCGGGAATC+TGG	+	chr2.4:26671034-26671053	None:intergenic	50.0%
	GTAACAGGGATCTGCTGAAC+AGG	+	chr2.4:26671065-26671084	None:intergenic	50.0%
	GTTCAGCAGATCCCTGTTAC+TGG	-	chr2.4:26671065-26671084	MS.gene039073:CDS	50.0%
!	ATCCCTGTTACTGGTGGTGT+TGG	-	chr2.4:26671074-26671093	MS.gene039073:CDS	50.0%
!	CTGTTACTGGTGGTGTTGGA+AGG	-	chr2.4:26671078-26671097	MS.gene039073:CDS	50.0%
!	CTTGACATGCTTGCTGCTCT+TGG	-	chr2.4:26671002-26671021	MS.gene039073:CDS	50.0%
!	TTACTGGTGGTGTTGGAAGG+AGG	-	chr2.4:26671081-26671100	MS.gene039073:CDS	50.0%
!!	GATTCCCGGTGTTGTTGTTC+AGG	-	chr2.4:26671034-26671053	MS.gene039073:CDS	50.0%
	CAGCAGATCCCTGTTACTGG+TGG	-	chr2.4:26671068-26671087	MS.gene039073:CDS	55.0%
!	GGAGGAGCGTGAGCGTCGTA+TGG	-	chr2.4:26670920-26670939	MS.gene039073:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	26670696	26671109	26670696	ID=MS.gene039073
chr2.4	mRNA	26670696	26671109	26670696	ID=MS.gene039073.t1;Parent=MS.gene039073
chr2.4	exon	26670696	26671109	26670696	ID=MS.gene039073.t1.exon1;Parent=MS.gene039073.t1
chr2.4	CDS	26670696	26671109	26670696	ID=cds.MS.gene039073.t1;Parent=MS.gene039073.t1

Gene Sequence

>MS.gene039073

ATGGGACGAGTCCGAACGAAAACCGTGAAGAAATCCTCTCGTCAAGTGATTGAGCGTTATTACTCGAAGATGACACCCGATTTCCACACGAACAAGAAGATCGTCGAAGAAGTTGCTTTGATACCATCAAAGCGTCTTCGTAACAAGATCGCTGGTTTCTCCACTCATCTCATGAAGCGTATTCAAAAGGGACCTGTTCGTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGAGCGTCGTATGGATTTCGTTCCTGATGTTTCTGCCATTAGGACCGACTATATTGAGGTTGATAAGGAAACCCTTGACATGCTTGCTGCTCTTGGTATGTCCCAGATTCCCGGTGTTGTTGTTCAGGTTGATCCTGTTCAGCAGATCCCTGTTACTGGTGGTGTTGGAAGGAGGTTTTAA

Protein sequence

>MS.gene039073.t1

MGRVRTKTVKKSSRQVIERYYSKMTPDFHTNKKIVEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDYIEVDKETLDMLAALGMSQIPGVVVQVDPVQQIPVTGGVGRRF