AlfalfaGEDB | MS.gene08343

M. sativa cultivar XinJiangDaYe / MS.gene08343

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08343.t1	XP_013467927.1	97.2	145	3	1	1	145	1	144	1.80E-68	268.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08343.t1	P49215	82.4	148	19	3	1	145	1	144	7.4e-58	224.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08343.t1	I3SPL5	97.2	145	3	1	1	145	1	144	1.3e-68	268.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056075	MS.gene08343	0.829369	5.35E-55	-1.69E-46
MS.gene056917	MS.gene08343	0.82225	2.62E-53	-1.69E-46
MS.gene056998	MS.gene08343	0.801967	7.01E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene08343	MS.gene023275	PPI
MS.gene034834	MS.gene08343	PPI
MS.gene028177	MS.gene08343	PPI
MS.gene08343	MS.gene056917	PPI
MS.gene08343	MS.gene039073	PPI
MS.gene08343	MS.gene028176	PPI
MS.gene08343	MS.gene039072	PPI
MS.gene08344	MS.gene08343	PPI
MS.gene08343	MS.gene040536	PPI
MS.gene08343	MS.gene54136	PPI
MS.gene08343	MS.gene34957	PPI
MS.gene034832	MS.gene08343	PPI
MS.gene08343	MS.gene08344	PPI
MS.gene08343	MS.gene023274	PPI
MS.gene065715	MS.gene08343	PPI
MS.gene08343	MS.gene065715	PPI
MS.gene34958	MS.gene08343	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08343.t1	MTR_1g058250	97.241	145	3	1	1	145	1	144	6.01e-100	283

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08343.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene08343.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene08343.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene08343.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene08343.t1	AT2G05220	75.839	149	23	2	1	145	1	140	2.28e-75	221
MS.gene08343.t1	AT2G05220	75.839	149	23	2	1	145	1	140	2.28e-75	221
MS.gene08343.t1	AT2G04390	78.912	147	23	3	1	145	1	141	2.81e-75	221
MS.gene08343.t1	AT3G10610	80.576	139	20	2	1	135	1	136	1.32e-74	219

Find 44 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATGATTTCTTCACGGTTT+TGG	0.256241	4.1:-16941420	None:intergenic
CGAGATTCCCGGTGTTGTTC+AGG	0.275016	4.1:+16941739	MS.gene08343:CDS
GGTGCTGCTGGTGGTGGTGC+TGG	0.304630	4.1:+16941806	MS.gene08343:CDS
CGTCTTCGCAACAAGATTGC+TGG	0.339095	4.1:+16941536	MS.gene08343:CDS
TTCCTGATGTTTCTGCTATT+AGG	0.341443	4.1:+16941657	MS.gene08343:CDS
TTCTTCAAGGATCTTCTTGT+TGG	0.343538	4.1:-16941492	None:intergenic
TTGTTGCGAAGACGCTTTGA+TGG	0.348895	4.1:-16941527	None:intergenic
CTGCTGGTGGTGGTGCTGGA+AGG	0.354715	4.1:+16941810	MS.gene08343:CDS
ATCCCATTCGGTCGTGGTGC+TGG	0.359143	4.1:+16941785	MS.gene08343:CDS
CCGAATGGGATCTGCTGAAC+AGG	0.361093	4.1:-16941773	None:intergenic
GGGATCTGCTGAACAGGAAC+AGG	0.372363	4.1:-16941767	None:intergenic
GGTCGTGGTGCTGGTGCTGC+TGG	0.373909	4.1:+16941794	MS.gene08343:CDS
CTTGGTATGTCCGAGATTCC+CGG	0.385581	4.1:+16941728	MS.gene08343:CDS
CCTTCTGAATACGTTTCATA+AGG	0.423532	4.1:-16941571	None:intergenic
CTAGACATGCTTGCTGCTCT+TGG	0.432500	4.1:+16941710	MS.gene08343:CDS
TTCAATGAAATTCCACGAAC+AGG	0.443778	4.1:-16941596	None:intergenic
GGTGCTGGTGCTGCTGGTGG+TGG	0.444507	4.1:+16941800	MS.gene08343:CDS
CTGAACAACACCGGGAATCT+CGG	0.461504	4.1:-16941738	None:intergenic
GAATCAAAGCAACTTCTTCA+AGG	0.471503	4.1:-16941505	None:intergenic
CCTGTTCAGCAGATCCCATT+CGG	0.473555	4.1:+16941773	MS.gene08343:CDS
CAAGAGCAGCAAGCATGTCT+AGG	0.477650	4.1:-16941709	None:intergenic
TGATCATATTGAGGTTGATA+AGG	0.481558	4.1:+16941682	MS.gene08343:CDS
CAAGGATCTTCTTGTTGGTG+TGG	0.483634	4.1:-16941487	None:intergenic
TTGACGAGATGATTTCTTCA+CGG	0.484691	4.1:-16941426	None:intergenic
CTGGTGGTGGTGCTGGAAGG+AGG	0.484753	4.1:+16941813	MS.gene08343:CDS
CACCAGCACCACGACCGAAT+GGG	0.505188	4.1:-16941787	None:intergenic
GTCCTAATAGCAGAAACATC+AGG	0.505221	4.1:-16941659	None:intergenic
GGAGGAGCGTGAGCGTCGTA+TGG	0.521163	4.1:+16941628	MS.gene08343:CDS
TTTGGTTCGGACTCGACCCA+TGG	0.522820	4.1:-16941402	None:intergenic
ATTCAGAAGGGACCTGTTCG+TGG	0.526647	4.1:+16941584	MS.gene08343:CDS
CGTGGTGCTGGTGCTGCTGG+TGG	0.530900	4.1:+16941797	MS.gene08343:CDS
CAGCAGATCCCATTCGGTCG+TGG	0.540344	4.1:+16941779	MS.gene08343:CDS
GTGGAAATCGAGTGTCATCT+TGG	0.542107	4.1:-16941468	None:intergenic
TATTAGGACTGATCATATTG+AGG	0.542204	4.1:+16941673	MS.gene08343:CDS
GCACCAGCACCACGACCGAA+TGG	0.558262	4.1:-16941788	None:intergenic
AAGAGCAGCAAGCATGTCTA+GGG	0.560169	4.1:-16941708	None:intergenic
TTCATTGAAGCTGCAAGAGG+AGG	0.567846	4.1:+16941610	MS.gene08343:CDS
CCTTATGAAACGTATTCAGA+AGG	0.588661	4.1:+16941571	MS.gene08343:CDS
AGGGTCAACCTGAACAACAC+CGG	0.593915	4.1:-16941747	None:intergenic
GGGTCAACCTGAACAACACC+GGG	0.608103	4.1:-16941746	None:intergenic
AATACGTTTCATAAGGTGAG+TGG	0.609703	4.1:-16941564	None:intergenic
AATTTCATTGAAGCTGCAAG+AGG	0.634258	4.1:+16941607	MS.gene08343:CDS
CTTATGAAACGTATTCAGAA+GGG	0.635445	4.1:+16941572	MS.gene08343:CDS
GGATCTGCTGAACAGGAACA+GGG	0.673645	4.1:-16941766	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTATGAAACGTATTCAGAA+GGG	+	chr4.1:16941572-16941591	MS.gene08343:CDS	30.0%
	TATTAGGACTGATCATATTG+AGG	+	chr4.1:16941673-16941692	MS.gene08343:CDS	30.0%
!!	TGATCATATTGAGGTTGATA+AGG	+	chr4.1:16941682-16941701	MS.gene08343:CDS	30.0%
	AATACGTTTCATAAGGTGAG+TGG	-	chr4.1:16941567-16941586	None:intergenic	35.0%
	AATTTCATTGAAGCTGCAAG+AGG	+	chr4.1:16941607-16941626	MS.gene08343:CDS	35.0%
	CCTTATGAAACGTATTCAGA+AGG	+	chr4.1:16941571-16941590	MS.gene08343:CDS	35.0%
	CCTTCTGAATACGTTTCATA+AGG	-	chr4.1:16941574-16941593	None:intergenic	35.0%
	GAATCAAAGCAACTTCTTCA+AGG	-	chr4.1:16941508-16941527	None:intergenic	35.0%
	TTCAATGAAATTCCACGAAC+AGG	-	chr4.1:16941599-16941618	None:intergenic	35.0%
	TTCTTCAAGGATCTTCTTGT+TGG	-	chr4.1:16941495-16941514	None:intergenic	35.0%
	TTGACGAGATGATTTCTTCA+CGG	-	chr4.1:16941429-16941448	None:intergenic	35.0%
!	AGATGATTTCTTCACGGTTT+TGG	-	chr4.1:16941423-16941442	None:intergenic	35.0%
!	TTCCTGATGTTTCTGCTATT+AGG	+	chr4.1:16941657-16941676	MS.gene08343:CDS	35.0%
!!!	ATTTCTTCACGGTTTTGGTT+CGG	-	chr4.1:16941418-16941437	None:intergenic	35.0%
	GTCCTAATAGCAGAAACATC+AGG	-	chr4.1:16941662-16941681	None:intergenic	40.0%
	AAGAGCAGCAAGCATGTCTA+GGG	-	chr4.1:16941711-16941730	None:intergenic	45.0%
	CAAGGATCTTCTTGTTGGTG+TGG	-	chr4.1:16941490-16941509	None:intergenic	45.0%
	GTGGAAATCGAGTGTCATCT+TGG	-	chr4.1:16941471-16941490	None:intergenic	45.0%
	TTCATTGAAGCTGCAAGAGG+AGG	+	chr4.1:16941610-16941629	MS.gene08343:CDS	45.0%
	TTGTTGCGAAGACGCTTTGA+TGG	-	chr4.1:16941530-16941549	None:intergenic	45.0%
	AGGGTCAACCTGAACAACAC+CGG	-	chr4.1:16941750-16941769	None:intergenic	50.0%
	ATTCAGAAGGGACCTGTTCG+TGG	+	chr4.1:16941584-16941603	MS.gene08343:CDS	50.0%
	CAAGAGCAGCAAGCATGTCT+AGG	-	chr4.1:16941712-16941731	None:intergenic	50.0%
	CCTGTTCAGCAGATCCCATT+CGG	+	chr4.1:16941773-16941792	MS.gene08343:CDS	50.0%
	CGTCTTCGCAACAAGATTGC+TGG	+	chr4.1:16941536-16941555	MS.gene08343:CDS	50.0%
	CTGAACAACACCGGGAATCT+CGG	-	chr4.1:16941741-16941760	None:intergenic	50.0%
	CTTGGTATGTCCGAGATTCC+CGG	+	chr4.1:16941728-16941747	MS.gene08343:CDS	50.0%
	GGATCTGCTGAACAGGAACA+GGG	-	chr4.1:16941769-16941788	None:intergenic	50.0%
!	CTAGACATGCTTGCTGCTCT+TGG	+	chr4.1:16941710-16941729	MS.gene08343:CDS	50.0%
	CCGAATGGGATCTGCTGAAC+AGG	-	chr4.1:16941776-16941795	None:intergenic	55.0%
	GGGATCTGCTGAACAGGAAC+AGG	-	chr4.1:16941770-16941789	None:intergenic	55.0%
	GGGTCAACCTGAACAACACC+GGG	-	chr4.1:16941749-16941768	None:intergenic	55.0%
!!	CGAGATTCCCGGTGTTGTTC+AGG	+	chr4.1:16941739-16941758	MS.gene08343:CDS	55.0%
	ATCCCATTCGGTCGTGGTGC+TGG	+	chr4.1:16941785-16941804	MS.gene08343:CDS	60.0%
	CACCAGCACCACGACCGAAT+GGG	-	chr4.1:16941790-16941809	None:intergenic	60.0%
	CAGCAGATCCCATTCGGTCG+TGG	+	chr4.1:16941779-16941798	MS.gene08343:CDS	60.0%
	GCACCAGCACCACGACCGAA+TGG	-	chr4.1:16941791-16941810	None:intergenic	65.0%
!	GGAGGAGCGTGAGCGTCGTA+TGG	+	chr4.1:16941628-16941647	MS.gene08343:CDS	65.0%
!!	CTGCTGGTGGTGGTGCTGGA+AGG	+	chr4.1:16941810-16941829	MS.gene08343:CDS	65.0%
!!	CTGGTGGTGGTGCTGGAAGG+AGG	+	chr4.1:16941813-16941832	MS.gene08343:CDS	65.0%
!!	CGTGGTGCTGGTGCTGCTGG+TGG	+	chr4.1:16941797-16941816	MS.gene08343:CDS	70.0%
!!	GGTCGTGGTGCTGGTGCTGC+TGG	+	chr4.1:16941794-16941813	MS.gene08343:CDS	70.0%
!!	GGTGCTGCTGGTGGTGGTGC+TGG	+	chr4.1:16941806-16941825	MS.gene08343:CDS	70.0%
!!	GGTGCTGGTGCTGCTGGTGG+TGG	+	chr4.1:16941800-16941819	MS.gene08343:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	16941404	16941841	16941404	ID=MS.gene08343
chr4.1	mRNA	16941404	16941841	16941404	ID=MS.gene08343.t1;Parent=MS.gene08343
chr4.1	exon	16941404	16941841	16941404	ID=MS.gene08343.t1.exon1;Parent=MS.gene08343.t1
chr4.1	CDS	16941404	16941841	16941404	ID=cds.MS.gene08343.t1;Parent=MS.gene08343.t1

Gene Sequence

>MS.gene08343

ATGGGTCGAGTCCGAACCAAAACCGTGAAGAAATCATCTCGTCAAGTCATTGAGCGTTATTACTCCAAGATGACACTCGATTTCCACACCAACAAGAAGATCCTTGAAGAAGTTGCTTTGATTCCATCAAAGCGTCTTCGCAACAAGATTGCTGGTTTTTCCACTCACCTTATGAAACGTATTCAGAAGGGACCTGTTCGTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGAGCGTCGTATGGACTTCGTTCCTGATGTTTCTGCTATTAGGACTGATCATATTGAGGTTGATAAGGAAACCCTAGACATGCTTGCTGCTCTTGGTATGTCCGAGATTCCCGGTGTTGTTCAGGTTGACCCTGTTCCTGTTCAGCAGATCCCATTCGGTCGTGGTGCTGGTGCTGCTGGTGGTGGTGCTGGAAGGAGGTTTTAA

Protein sequence

>MS.gene08343.t1

MGRVRTKTVKKSSRQVIERYYSKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDHIEVDKETLDMLAALGMSEIPGVVQVDPVPVQQIPFGRGAGAAGGGAGRRF