AlfalfaGEDB | MS.gene023275

M. sativa cultivar XinJiangDaYe / MS.gene023275

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023275.t1	XP_013467927.1	97.2	145	3	1	1	145	1	144	1.40E-68	268.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023275.t1	P49215	82.4	148	19	3	1	145	1	144	3.3e-58	225.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023275.t1	I3SPL5	97.2	145	3	1	1	145	1	144	1.0e-68	268.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene023275	MS.gene039072	PPI
MS.gene056917	MS.gene023275	PPI
MS.gene023275	MS.gene040536	PPI
MS.gene034832	MS.gene023275	PPI
MS.gene08343	MS.gene023275	PPI
MS.gene023275	MS.gene54136	PPI
MS.gene34958	MS.gene023275	PPI
MS.gene028176	MS.gene023275	PPI
MS.gene023275	MS.gene34957	PPI
MS.gene065715	MS.gene023275	PPI
MS.gene028177	MS.gene023275	PPI
MS.gene023275	MS.gene023274	PPI
MS.gene034834	MS.gene023275	PPI
MS.gene039073	MS.gene023275	PPI
MS.gene08344	MS.gene023275	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023275.t1	MTR_1g058250	97.241	145	3	1	1	145	1	144	6.07e-100	283

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023275.t1	AT5G04800	77.931	145	28	1	1	145	1	141	2.66e-76	223
MS.gene023275.t1	AT5G04800	77.931	145	28	1	1	145	1	141	2.66e-76	223
MS.gene023275.t1	AT5G04800	77.931	145	28	1	1	145	1	141	2.66e-76	223
MS.gene023275.t1	AT5G04800	77.931	145	28	1	1	145	1	141	2.66e-76	223
MS.gene023275.t1	AT2G04390	78.231	147	24	3	1	145	1	141	1.17e-74	219
MS.gene023275.t1	AT2G05220	75.168	149	24	2	1	145	1	140	1.18e-74	219
MS.gene023275.t1	AT2G05220	75.168	149	24	2	1	145	1	140	1.18e-74	219
MS.gene023275.t1	AT3G10610	80.576	139	20	2	1	135	1	136	1.28e-74	219

Find 44 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATGATTTCTTCACGGTTT+TGG	0.256241	4.3:-17448322	None:intergenic
CGAGATTCCCGGTGTTGTTC+AGG	0.275016	4.3:+17448641	MS.gene023275:CDS
CGTCTTCGCAACAAGATTGC+TGG	0.339095	4.3:+17448438	MS.gene023275:CDS
TTCCTGATGTTTCTGCTATT+AGG	0.341443	4.3:+17448559	MS.gene023275:CDS
TTCTTCAAGGATCTTCTTGT+TGG	0.343538	4.3:-17448394	None:intergenic
TTGTTGCGAAGACGCTTTGA+TGG	0.348895	4.3:-17448429	None:intergenic
ATCCCATTCGGTCGTGGTGC+TGG	0.359143	4.3:+17448687	MS.gene023275:CDS
CCGAATGGGATCTGCTGAAC+AGG	0.361093	4.3:-17448675	None:intergenic
GGTGCTGCTGGTGGTGGTGT+TGG	0.368534	4.3:+17448708	MS.gene023275:CDS
GGGATCTGCTGAACAGGAAC+AGG	0.372363	4.3:-17448669	None:intergenic
GGTCGTGGTGCTGGTGCTGC+TGG	0.373909	4.3:+17448696	MS.gene023275:CDS
CTGCTGGTGGTGGTGTTGGA+AGG	0.382557	4.3:+17448712	MS.gene023275:CDS
CTTGGTATGTCCGAGATTCC+CGG	0.385581	4.3:+17448630	MS.gene023275:CDS
CCTTCTGAATACGTTTCATA+AGG	0.423532	4.3:-17448473	None:intergenic
CTAGACATGCTTGCTGCTCT+TGG	0.432500	4.3:+17448612	MS.gene023275:CDS
TTCAATGAAATTCCACGAAC+AGG	0.443778	4.3:-17448498	None:intergenic
GGTGCTGGTGCTGCTGGTGG+TGG	0.444507	4.3:+17448702	MS.gene023275:CDS
CTGAACAACACCGGGAATCT+CGG	0.461504	4.3:-17448640	None:intergenic
GAATCAAAGCAACTTCTTCA+AGG	0.471503	4.3:-17448407	None:intergenic
CCTGTTCAGCAGATCCCATT+CGG	0.473555	4.3:+17448675	MS.gene023275:CDS
CAAGAGCAGCAAGCATGTCT+AGG	0.477650	4.3:-17448611	None:intergenic
TGATCATATTGAGGTTGATA+AGG	0.481558	4.3:+17448584	MS.gene023275:CDS
CAAGGATCTTCTTGTTGGTG+TGG	0.483634	4.3:-17448389	None:intergenic
TTGACGAGATGATTTCTTCA+CGG	0.484691	4.3:-17448328	None:intergenic
CACCAGCACCACGACCGAAT+GGG	0.505188	4.3:-17448689	None:intergenic
GTCCTAATAGCAGAAACATC+AGG	0.505221	4.3:-17448561	None:intergenic
CTGGTGGTGGTGTTGGAAGG+AGG	0.512573	4.3:+17448715	MS.gene023275:CDS
GGAGGAGCGTGAGCGTCGTA+TGG	0.521163	4.3:+17448530	MS.gene023275:CDS
TTTGGTTCGGACTCGACCCA+TGG	0.522820	4.3:-17448304	None:intergenic
ATTCAGAAGGGACCTGTTCG+TGG	0.526647	4.3:+17448486	MS.gene023275:CDS
CGTGGTGCTGGTGCTGCTGG+TGG	0.530900	4.3:+17448699	MS.gene023275:CDS
CAGCAGATCCCATTCGGTCG+TGG	0.540344	4.3:+17448681	MS.gene023275:CDS
GTGGAAATCGAGTGTCATCT+TGG	0.542107	4.3:-17448370	None:intergenic
TATTAGGACTGATCATATTG+AGG	0.542204	4.3:+17448575	MS.gene023275:CDS
GCACCAGCACCACGACCGAA+TGG	0.558262	4.3:-17448690	None:intergenic
AAGAGCAGCAAGCATGTCTA+GGG	0.560169	4.3:-17448610	None:intergenic
TTCATTGAAGCTGCAAGAGG+AGG	0.567846	4.3:+17448512	MS.gene023275:CDS
CCTTATGAAACGTATTCAGA+AGG	0.588661	4.3:+17448473	MS.gene023275:CDS
AGGGTCAACCTGAACAACAC+CGG	0.593915	4.3:-17448649	None:intergenic
GGGTCAACCTGAACAACACC+GGG	0.608103	4.3:-17448648	None:intergenic
AATACGTTTCATAAGGTGAG+TGG	0.609703	4.3:-17448466	None:intergenic
AATTTCATTGAAGCTGCAAG+AGG	0.634258	4.3:+17448509	MS.gene023275:CDS
CTTATGAAACGTATTCAGAA+GGG	0.635445	4.3:+17448474	MS.gene023275:CDS
GGATCTGCTGAACAGGAACA+GGG	0.673645	4.3:-17448668	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTATGAAACGTATTCAGAA+GGG	+	chr4.3:17448474-17448493	MS.gene023275:CDS	30.0%
	TATTAGGACTGATCATATTG+AGG	+	chr4.3:17448575-17448594	MS.gene023275:CDS	30.0%
!!	TGATCATATTGAGGTTGATA+AGG	+	chr4.3:17448584-17448603	MS.gene023275:CDS	30.0%
	AATACGTTTCATAAGGTGAG+TGG	-	chr4.3:17448469-17448488	None:intergenic	35.0%
	AATTTCATTGAAGCTGCAAG+AGG	+	chr4.3:17448509-17448528	MS.gene023275:CDS	35.0%
	CCTTATGAAACGTATTCAGA+AGG	+	chr4.3:17448473-17448492	MS.gene023275:CDS	35.0%
	CCTTCTGAATACGTTTCATA+AGG	-	chr4.3:17448476-17448495	None:intergenic	35.0%
	GAATCAAAGCAACTTCTTCA+AGG	-	chr4.3:17448410-17448429	None:intergenic	35.0%
	TTCAATGAAATTCCACGAAC+AGG	-	chr4.3:17448501-17448520	None:intergenic	35.0%
	TTCTTCAAGGATCTTCTTGT+TGG	-	chr4.3:17448397-17448416	None:intergenic	35.0%
	TTGACGAGATGATTTCTTCA+CGG	-	chr4.3:17448331-17448350	None:intergenic	35.0%
!	AGATGATTTCTTCACGGTTT+TGG	-	chr4.3:17448325-17448344	None:intergenic	35.0%
!	TTCCTGATGTTTCTGCTATT+AGG	+	chr4.3:17448559-17448578	MS.gene023275:CDS	35.0%
!!!	ATTTCTTCACGGTTTTGGTT+CGG	-	chr4.3:17448320-17448339	None:intergenic	35.0%
	GTCCTAATAGCAGAAACATC+AGG	-	chr4.3:17448564-17448583	None:intergenic	40.0%
	AAGAGCAGCAAGCATGTCTA+GGG	-	chr4.3:17448613-17448632	None:intergenic	45.0%
	CAAGGATCTTCTTGTTGGTG+TGG	-	chr4.3:17448392-17448411	None:intergenic	45.0%
	GTGGAAATCGAGTGTCATCT+TGG	-	chr4.3:17448373-17448392	None:intergenic	45.0%
	TTCATTGAAGCTGCAAGAGG+AGG	+	chr4.3:17448512-17448531	MS.gene023275:CDS	45.0%
	TTGTTGCGAAGACGCTTTGA+TGG	-	chr4.3:17448432-17448451	None:intergenic	45.0%
	AGGGTCAACCTGAACAACAC+CGG	-	chr4.3:17448652-17448671	None:intergenic	50.0%
	ATTCAGAAGGGACCTGTTCG+TGG	+	chr4.3:17448486-17448505	MS.gene023275:CDS	50.0%
	CAAGAGCAGCAAGCATGTCT+AGG	-	chr4.3:17448614-17448633	None:intergenic	50.0%
	CCTGTTCAGCAGATCCCATT+CGG	+	chr4.3:17448675-17448694	MS.gene023275:CDS	50.0%
	CGTCTTCGCAACAAGATTGC+TGG	+	chr4.3:17448438-17448457	MS.gene023275:CDS	50.0%
	CTGAACAACACCGGGAATCT+CGG	-	chr4.3:17448643-17448662	None:intergenic	50.0%
	CTTGGTATGTCCGAGATTCC+CGG	+	chr4.3:17448630-17448649	MS.gene023275:CDS	50.0%
	GGATCTGCTGAACAGGAACA+GGG	-	chr4.3:17448671-17448690	None:intergenic	50.0%
!	CTAGACATGCTTGCTGCTCT+TGG	+	chr4.3:17448612-17448631	MS.gene023275:CDS	50.0%
	CCGAATGGGATCTGCTGAAC+AGG	-	chr4.3:17448678-17448697	None:intergenic	55.0%
	GGGATCTGCTGAACAGGAAC+AGG	-	chr4.3:17448672-17448691	None:intergenic	55.0%
	GGGTCAACCTGAACAACACC+GGG	-	chr4.3:17448651-17448670	None:intergenic	55.0%
!!	CGAGATTCCCGGTGTTGTTC+AGG	+	chr4.3:17448641-17448660	MS.gene023275:CDS	55.0%
	ATCCCATTCGGTCGTGGTGC+TGG	+	chr4.3:17448687-17448706	MS.gene023275:CDS	60.0%
	CACCAGCACCACGACCGAAT+GGG	-	chr4.3:17448692-17448711	None:intergenic	60.0%
	CAGCAGATCCCATTCGGTCG+TGG	+	chr4.3:17448681-17448700	MS.gene023275:CDS	60.0%
!!	CTGCTGGTGGTGGTGTTGGA+AGG	+	chr4.3:17448712-17448731	MS.gene023275:CDS	60.0%
!!	CTGGTGGTGGTGTTGGAAGG+AGG	+	chr4.3:17448715-17448734	MS.gene023275:CDS	60.0%
	GCACCAGCACCACGACCGAA+TGG	-	chr4.3:17448693-17448712	None:intergenic	65.0%
!	GGAGGAGCGTGAGCGTCGTA+TGG	+	chr4.3:17448530-17448549	MS.gene023275:CDS	65.0%
!!	GGTGCTGCTGGTGGTGGTGT+TGG	+	chr4.3:17448708-17448727	MS.gene023275:CDS	65.0%
!!	CGTGGTGCTGGTGCTGCTGG+TGG	+	chr4.3:17448699-17448718	MS.gene023275:CDS	70.0%
!!	GGTCGTGGTGCTGGTGCTGC+TGG	+	chr4.3:17448696-17448715	MS.gene023275:CDS	70.0%
!!	GGTGCTGGTGCTGCTGGTGG+TGG	+	chr4.3:17448702-17448721	MS.gene023275:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	17448306	17448743	17448306	ID=MS.gene023275
chr4.3	mRNA	17448306	17448743	17448306	ID=MS.gene023275.t1;Parent=MS.gene023275
chr4.3	exon	17448306	17448743	17448306	ID=MS.gene023275.t1.exon1;Parent=MS.gene023275.t1
chr4.3	CDS	17448306	17448743	17448306	ID=cds.MS.gene023275.t1;Parent=MS.gene023275.t1

Gene Sequence

>MS.gene023275

ATGGGTCGAGTCCGAACCAAAACCGTGAAGAAATCATCTCGTCAAGTCATTGAGCGTTATTACTCCAAGATGACACTCGATTTCCACACCAACAAGAAGATCCTTGAAGAAGTTGCTTTGATTCCATCAAAGCGTCTTCGCAACAAGATTGCTGGTTTTTCCACTCACCTTATGAAACGTATTCAGAAGGGACCTGTTCGTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGAGCGTCGTATGGACTTCGTTCCTGATGTTTCTGCTATTAGGACTGATCATATTGAGGTTGATAAGGAAACCCTAGACATGCTTGCTGCTCTTGGTATGTCCGAGATTCCCGGTGTTGTTCAGGTTGACCCTGTTCCTGTTCAGCAGATCCCATTCGGTCGTGGTGCTGGTGCTGCTGGTGGTGGTGTTGGAAGGAGGTTTTAA

Protein sequence

>MS.gene023275.t1

MGRVRTKTVKKSSRQVIERYYSKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDHIEVDKETLDMLAALGMSEIPGVVQVDPVPVQQIPFGRGAGAAGGGVGRRF