AlfalfaGEDB | MS.gene34958

M. sativa cultivar XinJiangDaYe / MS.gene34958

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34958.t1	ACJ84115.1	88.9	144	12	1	1	140	1	144	1.20E-61	245.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34958.t1	P49215	77.8	144	28	3	1	140	1	144	1.5e-52	206.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34958.t1	B7FH86	88.9	144	12	1	1	140	1	144	8.9e-62	245.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene34958	MS.gene023275	PPI
MS.gene34958	MS.gene028177	PPI
MS.gene34958	MS.gene039072	PPI
MS.gene34958	MS.gene056917	PPI
MS.gene34958	MS.gene039073	PPI
MS.gene028177	MS.gene34958	PPI
MS.gene34958	MS.gene34957	PPI
MS.gene34958	MS.gene034832	PPI
MS.gene34958	MS.gene028176	PPI
MS.gene34958	MS.gene08344	PPI
MS.gene34958	MS.gene040536	PPI
MS.gene34958	MS.gene54136	PPI
MS.gene34958	MS.gene034834	PPI
MS.gene34958	MS.gene08343	PPI
MS.gene34958	MS.gene023274	PPI
MS.gene34958	MS.gene065715	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34958.t1	MTR_1g058250	92.241	116	9	0	1	116	1	116	4.94e-74	217

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34958.t1	AT5G04800	72.535	142	36	2	1	140	1	141	1.62e-68	203
MS.gene34958.t1	AT5G04800	72.535	142	36	2	1	140	1	141	1.62e-68	203
MS.gene34958.t1	AT5G04800	72.535	142	36	2	1	140	1	141	1.62e-68	203
MS.gene34958.t1	AT5G04800	72.535	142	36	2	1	140	1	141	1.62e-68	203
MS.gene34958.t1	AT3G10610	72.857	140	37	1	1	139	1	140	7.33e-68	202
MS.gene34958.t1	AT2G04390	73.050	141	37	1	1	140	1	141	1.16e-67	201
MS.gene34958.t1	AT2G05220	72.143	140	39	0	1	140	1	140	4.38e-67	199
MS.gene34958.t1	AT2G05220	72.143	140	39	0	1	140	1	140	4.38e-67	199

Find 35 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGGATTTCTTCACGGTTT+TGG	0.259892	4.4:-17787025	None:intergenic
ATTCGAAAGGGACCTGTTCT+TGG	0.302463	4.4:+17787189	MS.gene34958:CDS
TTCCTGATGTTTCTGCTATT+AGG	0.341443	4.4:+17787262	MS.gene34958:CDS
GAAGATTCCTGGTGTTGTTC+AGG	0.347999	4.4:+17787344	MS.gene34958:CDS
CGTCTTCGTAACAAGATTGC+TGG	0.356034	4.4:+17787141	MS.gene34958:CDS
CAAGAGCAGCAAGCATATCT+AGG	0.361317	4.4:-17787314	None:intergenic
CTTGGCATGTCGAAGATTCC+TGG	0.368757	4.4:+17787333	MS.gene34958:CDS
TTCTTCAAGGATCTTCTTGT+TGG	0.377402	4.4:-17787097	None:intergenic
TTCAATGAAATTCCAAGAAC+AGG	0.386161	4.4:-17787201	None:intergenic
CTAGATATGCTTGCTGCTCT+TGG	0.395976	4.4:+17787315	MS.gene34958:CDS
GCACCAGCACCACGACCATA+TGG	0.428749	4.4:-17787393	None:intergenic
ATCCCATATGGTCGTGGTGC+TGG	0.431174	4.4:+17787390	MS.gene34958:CDS
TATGGTCGTGGTGCTGGTGC+TGG	0.432732	4.4:+17787396	MS.gene34958:CDS
TTGTTACGAAGACGCTTTGA+AGG	0.464587	4.4:-17787132	None:intergenic
GGATCAAAGCGACTTCTTCA+AGG	0.477079	4.4:-17787110	None:intergenic
CTTGTTCAGCAGATCCCATA+TGG	0.480117	4.4:+17787378	MS.gene34958:CDS
TGATCATATTGAGGTTGATA+AGG	0.481558	4.4:+17787287	MS.gene34958:CDS
GTCCTAATAGCAGAAACATC+AGG	0.505221	4.4:-17787264	None:intergenic
TTTGGTTCGGACTCGTCCCA+TGG	0.523667	4.4:-17787007	None:intergenic
TTGACGAGAGGATTTCTTCA+CGG	0.524605	4.4:-17787031	None:intergenic
GGAGGAGCGTGATCGTCGTA+TGG	0.525336	4.4:+17787233	MS.gene34958:CDS
TATTAGGACTGATCATATTG+AGG	0.542204	4.4:+17787278	MS.gene34958:CDS
GTGGTGCTGGTGCTGGAAGG+AGG	0.550588	4.4:+17787403	MS.gene34958:CDS
CACCAGCACCACGACCATAT+GGG	0.556729	4.4:-17787392	None:intergenic
CTCATGCAGCGTATTCGAAA+GGG	0.564195	4.4:+17787177	MS.gene34958:CDS
GTCGTGGTGCTGGTGCTGGA+AGG	0.566651	4.4:+17787400	MS.gene34958:CDS
TTCATTGAAGCTGCAAGAGG+AGG	0.567846	4.4:+17787215	MS.gene34958:CDS
CGATTAACCTGAACAACACC+AGG	0.574528	4.4:-17787351	None:intergenic
CAGCAGATCCCATATGGTCG+TGG	0.574554	4.4:+17787384	MS.gene34958:CDS
AAGAGCAGCAAGCATATCTA+GGG	0.580550	4.4:-17787313	None:intergenic
TCTCATGCAGCGTATTCGAA+AGG	0.588377	4.4:+17787176	MS.gene34958:CDS
TGTTACGAAGACGCTTTGAA+GGG	0.597110	4.4:-17787131	None:intergenic
ACGCTCGATGATTTGACGAG+AGG	0.611995	4.4:-17787043	None:intergenic
AATTTCATTGAAGCTGCAAG+AGG	0.615547	4.4:+17787212	MS.gene34958:CDS
TAACCACCCTAAAATGACAC+TGG	0.617615	4.4:+17787065	MS.gene34958:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TATTAGGACTGATCATATTG+AGG	+	chr4.4:17787278-17787297	MS.gene34958:CDS	30.0%
	TTCAATGAAATTCCAAGAAC+AGG	-	chr4.4:17787204-17787223	None:intergenic	30.0%
!!	TGATCATATTGAGGTTGATA+AGG	+	chr4.4:17787287-17787306	MS.gene34958:CDS	30.0%
!!!	GAAAATCCAGTGTCATTTTA+GGG	-	chr4.4:17787074-17787093	None:intergenic	30.0%
!!!	TGAAAATCCAGTGTCATTTT+AGG	-	chr4.4:17787075-17787094	None:intergenic	30.0%
	AATTTCATTGAAGCTGCAAG+AGG	+	chr4.4:17787212-17787231	MS.gene34958:CDS	35.0%
	TTCTTCAAGGATCTTCTTGT+TGG	-	chr4.4:17787100-17787119	None:intergenic	35.0%
!	TTCCTGATGTTTCTGCTATT+AGG	+	chr4.4:17787262-17787281	MS.gene34958:CDS	35.0%
!!!	ATTTCTTCACGGTTTTGGTT+CGG	-	chr4.4:17787023-17787042	None:intergenic	35.0%
	AAGAGCAGCAAGCATATCTA+GGG	-	chr4.4:17787316-17787335	None:intergenic	40.0%
	GTCCTAATAGCAGAAACATC+AGG	-	chr4.4:17787267-17787286	None:intergenic	40.0%
	TAACCACCCTAAAATGACAC+TGG	+	chr4.4:17787065-17787084	MS.gene34958:CDS	40.0%
	TTGACGAGAGGATTTCTTCA+CGG	-	chr4.4:17787034-17787053	None:intergenic	40.0%
	TTGTTACGAAGACGCTTTGA+AGG	-	chr4.4:17787135-17787154	None:intergenic	40.0%
!	AGAGGATTTCTTCACGGTTT+TGG	-	chr4.4:17787028-17787047	None:intergenic	40.0%
!	TGTTACGAAGACGCTTTGAA+GGG	-	chr4.4:17787134-17787153	None:intergenic	40.0%
!!!	AATCCAGTGTCATTTTAGGG+TGG	-	chr4.4:17787071-17787090	None:intergenic	40.0%
	ATTCGAAAGGGACCTGTTCT+TGG	+	chr4.4:17787189-17787208	MS.gene34958:CDS	45.0%
	CAAGAGCAGCAAGCATATCT+AGG	-	chr4.4:17787317-17787336	None:intergenic	45.0%
	CGATTAACCTGAACAACACC+AGG	-	chr4.4:17787354-17787373	None:intergenic	45.0%
	CGTCTTCGTAACAAGATTGC+TGG	+	chr4.4:17787141-17787160	MS.gene34958:CDS	45.0%
	CTCATGCAGCGTATTCGAAA+GGG	+	chr4.4:17787177-17787196	MS.gene34958:CDS	45.0%
	CTTGTTCAGCAGATCCCATA+TGG	+	chr4.4:17787378-17787397	MS.gene34958:CDS	45.0%
	GGATCAAAGCGACTTCTTCA+AGG	-	chr4.4:17787113-17787132	None:intergenic	45.0%
	TCTCATGCAGCGTATTCGAA+AGG	+	chr4.4:17787176-17787195	MS.gene34958:CDS	45.0%
	TTCATTGAAGCTGCAAGAGG+AGG	+	chr4.4:17787215-17787234	MS.gene34958:CDS	45.0%
!	CTAGATATGCTTGCTGCTCT+TGG	+	chr4.4:17787315-17787334	MS.gene34958:CDS	45.0%
!!	GAAGATTCCTGGTGTTGTTC+AGG	+	chr4.4:17787344-17787363	MS.gene34958:CDS	45.0%
	ACGCTCGATGATTTGACGAG+AGG	-	chr4.4:17787046-17787065	None:intergenic	50.0%
	CTTGGCATGTCGAAGATTCC+TGG	+	chr4.4:17787333-17787352	MS.gene34958:CDS	50.0%
	ATCCCATATGGTCGTGGTGC+TGG	+	chr4.4:17787390-17787409	MS.gene34958:CDS	55.0%
	CACCAGCACCACGACCATAT+GGG	-	chr4.4:17787395-17787414	None:intergenic	55.0%
	CAGCAGATCCCATATGGTCG+TGG	+	chr4.4:17787384-17787403	MS.gene34958:CDS	55.0%
	GCACCAGCACCACGACCATA+TGG	-	chr4.4:17787396-17787415	None:intergenic	60.0%
!	GGAGGAGCGTGATCGTCGTA+TGG	+	chr4.4:17787233-17787252	MS.gene34958:CDS	60.0%
!!	TATGGTCGTGGTGCTGGTGC+TGG	+	chr4.4:17787396-17787415	MS.gene34958:CDS	60.0%
!!	GTCGTGGTGCTGGTGCTGGA+AGG	+	chr4.4:17787400-17787419	MS.gene34958:CDS	65.0%
!!	GTGGTGCTGGTGCTGGAAGG+AGG	+	chr4.4:17787403-17787422	MS.gene34958:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	17787009	17787431	17787009	ID=MS.gene34958
chr4.4	mRNA	17787009	17787431	17787009	ID=MS.gene34958.t1;Parent=MS.gene34958
chr4.4	exon	17787009	17787431	17787009	ID=MS.gene34958.t1.exon1;Parent=MS.gene34958.t1
chr4.4	CDS	17787009	17787431	17787009	ID=cds.MS.gene34958.t1;Parent=MS.gene34958.t1

Gene Sequence

>MS.gene34958

ATGGGACGAGTCCGAACCAAAACCGTGAAGAAATCCTCTCGTCAAATCATCGAGCGTAACCACCCTAAAATGACACTGGATTTTCACACCAACAAGAAGATCCTTGAAGAAGTCGCTTTGATCCCTTCAAAGCGTCTTCGTAACAAGATTGCTGGTTTCTCTACGCATCTCATGCAGCGTATTCGAAAGGGACCTGTTCTTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGATCGTCGTATGGATTTCGTTCCTGATGTTTCTGCTATTAGGACTGATCATATTGAGGTTGATAAGGAAACCCTAGATATGCTTGCTGCTCTTGGCATGTCGAAGATTCCTGGTGTTGTTCAGGTTAATCGTGTTCTTGTTCAGCAGATCCCATATGGTCGTGGTGCTGGTGCTGGAAGGAGGTTTTAA

Protein sequence

>MS.gene34958.t1

MGRVRTKTVKKSSRQIIERNHPKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMQRIRKGPVLGISLKLQEEERDRRMDFVPDVSAIRTDHIEVDKETLDMLAALGMSKIPGVVQVNRVLVQQIPYGRGAGAGRRF