AlfalfaGEDB | MS.gene34957

M. sativa cultivar XinJiangDaYe / MS.gene34957

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34957.t1	XP_013467927.1	97.2	145	3	1	1	145	1	144	1.80E-68	268.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34957.t1	P49215	82.4	148	19	3	1	145	1	144	7.4e-58	224.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34957.t1	I3SPL5	97.2	145	3	1	1	145	1	144	1.3e-68	268.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050912	MS.gene34957	0.82081	5.63E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene34957	MS.gene040536	PPI
MS.gene039073	MS.gene34957	PPI
MS.gene34957	MS.gene54136	PPI
MS.gene08344	MS.gene34957	PPI
MS.gene034834	MS.gene34957	PPI
MS.gene023275	MS.gene34957	PPI
MS.gene056917	MS.gene34957	PPI
MS.gene34957	MS.gene023274	PPI
MS.gene034832	MS.gene34957	PPI
MS.gene08343	MS.gene34957	PPI
MS.gene34958	MS.gene34957	PPI
MS.gene028176	MS.gene34957	PPI
MS.gene039072	MS.gene34957	PPI
MS.gene065715	MS.gene34957	PPI
MS.gene028177	MS.gene34957	PPI
MS.gene34957	MS.gene039072	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34957.t1	MTR_1g058250	97.241	145	3	1	1	145	1	144	6.01e-100	283

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34957.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene34957.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene34957.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene34957.t1	AT5G04800	78.621	145	27	1	1	145	1	141	5.25e-77	225
MS.gene34957.t1	AT2G05220	75.839	149	23	2	1	145	1	140	2.28e-75	221
MS.gene34957.t1	AT2G05220	75.839	149	23	2	1	145	1	140	2.28e-75	221
MS.gene34957.t1	AT2G04390	78.912	147	23	3	1	145	1	141	2.81e-75	221
MS.gene34957.t1	AT3G10610	80.576	139	20	2	1	135	1	136	1.32e-74	219

Find 44 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATGATTTCTTCACGGTTT+TGG	0.256241	4.4:-17778378	None:intergenic
CGAGATTCCCGGTGTTGTTC+AGG	0.275016	4.4:+17778697	MS.gene34957:CDS
GGTGCTGCTGGTGGTGGTGC+TGG	0.304630	4.4:+17778764	MS.gene34957:CDS
CGTCTTCGCAACAAGATTGC+TGG	0.339095	4.4:+17778494	MS.gene34957:CDS
TTCCTGATGTTTCTGCTATT+AGG	0.341443	4.4:+17778615	MS.gene34957:CDS
TTCTTCAAGGATCTTCTTGT+TGG	0.343538	4.4:-17778450	None:intergenic
TTGTTGCGAAGACGCTTTGA+TGG	0.348895	4.4:-17778485	None:intergenic
CTGCTGGTGGTGGTGCTGGA+AGG	0.354715	4.4:+17778768	MS.gene34957:CDS
GGGATCTGCTGAACAGGAAC+AGG	0.359204	4.4:-17778725	None:intergenic
CCAAATGGGATCTGCTGAAC+AGG	0.361093	4.4:-17778731	None:intergenic
CCTGTTCAGCAGATCCCATT+TGG	0.377310	4.4:+17778731	MS.gene34957:CDS
CTTGGTATGTCCGAGATTCC+CGG	0.385581	4.4:+17778686	MS.gene34957:CDS
GGTCGTGGTGCTGGTGCTGC+TGG	0.397665	4.4:+17778752	MS.gene34957:CDS
ATCCCATTTGGTCGTGGTGC+TGG	0.404490	4.4:+17778743	MS.gene34957:CDS
CCTTCTGAATACGTTTCATA+AGG	0.423532	4.4:-17778529	None:intergenic
CTAGACATGCTTGCTGCTCT+TGG	0.432500	4.4:+17778668	MS.gene34957:CDS
GCACCAGCACCACGACCAAA+TGG	0.436244	4.4:-17778746	None:intergenic
TTCAATGAAATTCCACGAAC+AGG	0.443778	4.4:-17778554	None:intergenic
GGTGCTGGTGCTGCTGGTGG+TGG	0.444507	4.4:+17778758	MS.gene34957:CDS
CTGAACAACACCGGGAATCT+CGG	0.461504	4.4:-17778696	None:intergenic
GAATCAAAGCAACTTCTTCA+AGG	0.471503	4.4:-17778463	None:intergenic
CAAGAGCAGCAAGCATGTCT+AGG	0.477650	4.4:-17778667	None:intergenic
TGATCATATTGAGGTTGATA+AGG	0.481558	4.4:+17778640	MS.gene34957:CDS
CAAGGATCTTCTTGTTGGTG+TGG	0.483634	4.4:-17778445	None:intergenic
TTGACGAGATGATTTCTTCA+CGG	0.484691	4.4:-17778384	None:intergenic
CTGGTGGTGGTGCTGGAAGG+AGG	0.484753	4.4:+17778771	MS.gene34957:CDS
GTCCTAATAGCAGAAACATC+AGG	0.505221	4.4:-17778617	None:intergenic
GGAGGAGCGTGAGCGTCGTA+TGG	0.521163	4.4:+17778586	MS.gene34957:CDS
TTTGGTTCGGACTCGACCCA+TGG	0.522820	4.4:-17778360	None:intergenic
CGTGGTGCTGGTGCTGCTGG+TGG	0.525996	4.4:+17778755	MS.gene34957:CDS
ATTCAGAAGGGACCTGTTCG+TGG	0.526647	4.4:+17778542	MS.gene34957:CDS
CACCAGCACCACGACCAAAT+GGG	0.535186	4.4:-17778745	None:intergenic
CAGCAGATCCCATTTGGTCG+TGG	0.539254	4.4:+17778737	MS.gene34957:CDS
GTGGAAATCGAGTGTCATCT+TGG	0.542107	4.4:-17778426	None:intergenic
TATTAGGACTGATCATATTG+AGG	0.542204	4.4:+17778631	MS.gene34957:CDS
AAGAGCAGCAAGCATGTCTA+GGG	0.560169	4.4:-17778666	None:intergenic
TTCATTGAAGCTGCAAGAGG+AGG	0.567846	4.4:+17778568	MS.gene34957:CDS
CCTTATGAAACGTATTCAGA+AGG	0.588661	4.4:+17778529	MS.gene34957:CDS
AGGGTCAACCTGAACAACAC+CGG	0.593915	4.4:-17778705	None:intergenic
GGGTCAACCTGAACAACACC+GGG	0.608103	4.4:-17778704	None:intergenic
AATACGTTTCATAAGGTGAG+TGG	0.609703	4.4:-17778522	None:intergenic
AATTTCATTGAAGCTGCAAG+AGG	0.634258	4.4:+17778565	MS.gene34957:CDS
CTTATGAAACGTATTCAGAA+GGG	0.635445	4.4:+17778530	MS.gene34957:CDS
GGATCTGCTGAACAGGAACA+GGG	0.673645	4.4:-17778724	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTATGAAACGTATTCAGAA+GGG	+	chr4.4:17778530-17778549	MS.gene34957:CDS	30.0%
	TATTAGGACTGATCATATTG+AGG	+	chr4.4:17778631-17778650	MS.gene34957:CDS	30.0%
!!	TGATCATATTGAGGTTGATA+AGG	+	chr4.4:17778640-17778659	MS.gene34957:CDS	30.0%
	AATACGTTTCATAAGGTGAG+TGG	-	chr4.4:17778525-17778544	None:intergenic	35.0%
	AATTTCATTGAAGCTGCAAG+AGG	+	chr4.4:17778565-17778584	MS.gene34957:CDS	35.0%
	CCTTATGAAACGTATTCAGA+AGG	+	chr4.4:17778529-17778548	MS.gene34957:CDS	35.0%
	CCTTCTGAATACGTTTCATA+AGG	-	chr4.4:17778532-17778551	None:intergenic	35.0%
	GAATCAAAGCAACTTCTTCA+AGG	-	chr4.4:17778466-17778485	None:intergenic	35.0%
	TTCAATGAAATTCCACGAAC+AGG	-	chr4.4:17778557-17778576	None:intergenic	35.0%
	TTCTTCAAGGATCTTCTTGT+TGG	-	chr4.4:17778453-17778472	None:intergenic	35.0%
	TTGACGAGATGATTTCTTCA+CGG	-	chr4.4:17778387-17778406	None:intergenic	35.0%
!	AGATGATTTCTTCACGGTTT+TGG	-	chr4.4:17778381-17778400	None:intergenic	35.0%
!	TTCCTGATGTTTCTGCTATT+AGG	+	chr4.4:17778615-17778634	MS.gene34957:CDS	35.0%
!!!	ATTTCTTCACGGTTTTGGTT+CGG	-	chr4.4:17778376-17778395	None:intergenic	35.0%
	GTCCTAATAGCAGAAACATC+AGG	-	chr4.4:17778620-17778639	None:intergenic	40.0%
	AAGAGCAGCAAGCATGTCTA+GGG	-	chr4.4:17778669-17778688	None:intergenic	45.0%
	CAAGGATCTTCTTGTTGGTG+TGG	-	chr4.4:17778448-17778467	None:intergenic	45.0%
	GTGGAAATCGAGTGTCATCT+TGG	-	chr4.4:17778429-17778448	None:intergenic	45.0%
	TTCATTGAAGCTGCAAGAGG+AGG	+	chr4.4:17778568-17778587	MS.gene34957:CDS	45.0%
	TTGTTGCGAAGACGCTTTGA+TGG	-	chr4.4:17778488-17778507	None:intergenic	45.0%
	AGGGTCAACCTGAACAACAC+CGG	-	chr4.4:17778708-17778727	None:intergenic	50.0%
	ATTCAGAAGGGACCTGTTCG+TGG	+	chr4.4:17778542-17778561	MS.gene34957:CDS	50.0%
	CAAGAGCAGCAAGCATGTCT+AGG	-	chr4.4:17778670-17778689	None:intergenic	50.0%
	CCAAATGGGATCTGCTGAAC+AGG	-	chr4.4:17778734-17778753	None:intergenic	50.0%
	CCTGTTCAGCAGATCCCATT+TGG	+	chr4.4:17778731-17778750	MS.gene34957:CDS	50.0%
	CGTCTTCGCAACAAGATTGC+TGG	+	chr4.4:17778494-17778513	MS.gene34957:CDS	50.0%
	CTGAACAACACCGGGAATCT+CGG	-	chr4.4:17778699-17778718	None:intergenic	50.0%
	CTTGGTATGTCCGAGATTCC+CGG	+	chr4.4:17778686-17778705	MS.gene34957:CDS	50.0%
	GGATCTGCTGAACAGGAACA+GGG	-	chr4.4:17778727-17778746	None:intergenic	50.0%
!	CTAGACATGCTTGCTGCTCT+TGG	+	chr4.4:17778668-17778687	MS.gene34957:CDS	50.0%
	ATCCCATTTGGTCGTGGTGC+TGG	+	chr4.4:17778743-17778762	MS.gene34957:CDS	55.0%
	CACCAGCACCACGACCAAAT+GGG	-	chr4.4:17778748-17778767	None:intergenic	55.0%
	CAGCAGATCCCATTTGGTCG+TGG	+	chr4.4:17778737-17778756	MS.gene34957:CDS	55.0%
	GGGATCTGCTGAACAGGAAC+AGG	-	chr4.4:17778728-17778747	None:intergenic	55.0%
	GGGTCAACCTGAACAACACC+GGG	-	chr4.4:17778707-17778726	None:intergenic	55.0%
!!	CGAGATTCCCGGTGTTGTTC+AGG	+	chr4.4:17778697-17778716	MS.gene34957:CDS	55.0%
	GCACCAGCACCACGACCAAA+TGG	-	chr4.4:17778749-17778768	None:intergenic	60.0%
!	GGAGGAGCGTGAGCGTCGTA+TGG	+	chr4.4:17778586-17778605	MS.gene34957:CDS	65.0%
!!	CTGCTGGTGGTGGTGCTGGA+AGG	+	chr4.4:17778768-17778787	MS.gene34957:CDS	65.0%
!!	CTGGTGGTGGTGCTGGAAGG+AGG	+	chr4.4:17778771-17778790	MS.gene34957:CDS	65.0%
!!	CGTGGTGCTGGTGCTGCTGG+TGG	+	chr4.4:17778755-17778774	MS.gene34957:CDS	70.0%
!!	GGTCGTGGTGCTGGTGCTGC+TGG	+	chr4.4:17778752-17778771	MS.gene34957:CDS	70.0%
!!	GGTGCTGCTGGTGGTGGTGC+TGG	+	chr4.4:17778764-17778783	MS.gene34957:CDS	70.0%
!!	GGTGCTGGTGCTGCTGGTGG+TGG	+	chr4.4:17778758-17778777	MS.gene34957:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	17778362	17778799	17778362	ID=MS.gene34957
chr4.4	mRNA	17778362	17778799	17778362	ID=MS.gene34957.t1;Parent=MS.gene34957
chr4.4	exon	17778362	17778799	17778362	ID=MS.gene34957.t1.exon1;Parent=MS.gene34957.t1
chr4.4	CDS	17778362	17778799	17778362	ID=cds.MS.gene34957.t1;Parent=MS.gene34957.t1

Gene Sequence

>MS.gene34957

ATGGGTCGAGTCCGAACCAAAACCGTGAAGAAATCATCTCGTCAAGTCATTGAGCGTTATTACTCCAAGATGACACTCGATTTCCACACCAACAAGAAGATCCTTGAAGAAGTTGCTTTGATTCCATCAAAGCGTCTTCGCAACAAGATTGCTGGTTTTTCCACTCACCTTATGAAACGTATTCAGAAGGGACCTGTTCGTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGAGCGTCGTATGGACTTCGTTCCTGATGTTTCTGCTATTAGGACTGATCATATTGAGGTTGATAAGGAAACCCTAGACATGCTTGCTGCTCTTGGTATGTCCGAGATTCCCGGTGTTGTTCAGGTTGACCCTGTTCCTGTTCAGCAGATCCCATTTGGTCGTGGTGCTGGTGCTGCTGGTGGTGGTGCTGGAAGGAGGTTTTAA

Protein sequence

>MS.gene34957.t1

MGRVRTKTVKKSSRQVIERYYSKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDHIEVDKETLDMLAALGMSEIPGVVQVDPVPVQQIPFGRGAGAAGGGAGRRF