Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026677.t1 | RHN77875.1 | 96.3 | 573 | 16 | 3 | 1 | 570 | 34 | 604 | 1.70E-303 | 1051.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026677.t1 | Q8L7M4 | 61.6 | 573 | 209 | 4 | 1 | 569 | 1 | 566 | 2.4e-197 | 689.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026677.t1 | A0A072VFB2 | 96.3 | 573 | 16 | 3 | 1 | 570 | 1 | 571 | 1.2e-303 | 1051.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene026677 | MS.gene061619 | PPI |
| MS.gene27003 | MS.gene026677 | PPI |
| MS.gene026677 | MS.gene24232 | PPI |
| MS.gene026677 | MS.gene90863 | PPI |
| MS.gene36891 | MS.gene026677 | PPI |
| MS.gene026677 | MS.gene063717 | PPI |
| MS.gene026677 | MS.gene83189 | PPI |
| MS.gene073885 | MS.gene026677 | PPI |
| MS.gene63685 | MS.gene026677 | PPI |
| MS.gene026677 | MS.gene75146 | PPI |
| MS.gene037409 | MS.gene026677 | PPI |
| MS.gene026677 | MS.gene27265 | PPI |
| MS.gene026677 | MS.gene90723 | PPI |
| MS.gene026677 | MS.gene053439 | PPI |
| MS.gene018285 | MS.gene026677 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026677.t1 | MTR_1g029410 | 96.335 | 573 | 16 | 3 | 1 | 570 | 1 | 571 | 0.0 | 1114 |
| MS.gene026677.t1 | MTR_1g029410 | 97.059 | 374 | 8 | 2 | 1 | 371 | 38 | 411 | 0.0 | 738 |
| MS.gene026677.t1 | MTR_1g023955 | 45.912 | 159 | 84 | 2 | 215 | 371 | 142 | 300 | 4.30e-33 | 133 |
| MS.gene026677.t1 | MTR_2g100280 | 31.737 | 167 | 91 | 6 | 216 | 363 | 139 | 301 | 3.91e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026677.t1 | AT5G25800 | 61.498 | 574 | 210 | 4 | 1 | 570 | 1 | 567 | 0.0 | 729 |
| MS.gene026677.t1 | AT5G25800 | 61.111 | 576 | 211 | 5 | 1 | 570 | 1 | 569 | 0.0 | 723 |
| MS.gene026677.t1 | AT3G50100 | 47.712 | 153 | 78 | 2 | 215 | 365 | 43 | 195 | 3.08e-35 | 135 |
| MS.gene026677.t1 | AT3G50100 | 47.712 | 153 | 78 | 2 | 215 | 365 | 140 | 292 | 3.11e-34 | 134 |
| MS.gene026677.t1 | AT5G05540 | 40.426 | 188 | 103 | 4 | 193 | 371 | 118 | 305 | 1.26e-33 | 131 |
| MS.gene026677.t1 | AT5G05540 | 40.426 | 188 | 103 | 4 | 193 | 371 | 118 | 305 | 7.27e-33 | 132 |
| MS.gene026677.t1 | AT5G67240 | 39.521 | 167 | 99 | 2 | 215 | 379 | 145 | 311 | 1.58e-29 | 122 |
| MS.gene026677.t1 | AT5G67240 | 39.521 | 167 | 99 | 2 | 215 | 379 | 145 | 311 | 2.76e-29 | 123 |
| MS.gene026677.t1 | AT5G67240 | 39.521 | 167 | 99 | 2 | 215 | 379 | 145 | 311 | 4.10e-29 | 122 |
| MS.gene026677.t1 | AT5G67240 | 39.521 | 167 | 99 | 2 | 215 | 379 | 145 | 311 | 4.92e-29 | 122 |
| MS.gene026677.t1 | AT3G50090 | 37.931 | 145 | 75 | 3 | 214 | 356 | 74 | 205 | 2.44e-21 | 95.5 |
| MS.gene026677.t1 | AT3G15080 | 35.196 | 179 | 107 | 6 | 189 | 363 | 57 | 230 | 1.14e-15 | 78.2 |
Find 106 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATTTCTATGACGTTTA+TGG | 0.145380 | 1.2:+15295738 | MS.gene026677:CDS |
| GGAAAGCTTGGGAGAGTTTC+AGG | 0.157341 | 1.2:+15304658 | MS.gene026677:CDS |
| TATTCTTGTTGGGCATTCTT+TGG | 0.227921 | 1.2:+15301451 | MS.gene026677:CDS |
| CATGCAAGCAGAAAAGAAAA+AGG | 0.256388 | 1.2:+15298522 | MS.gene026677:CDS |
| GTTAAGGTATAGTATGTAAC+AGG | 0.298427 | 1.2:-15298949 | None:intergenic |
| AAATTACCTGGCTTATTAAC+AGG | 0.303767 | 1.2:-15299000 | None:intergenic |
| GACAAGTTTGACAGACATTC+AGG | 0.313900 | 1.2:+15301278 | MS.gene026677:CDS |
| GAGGAGGGTGAAAGTAATTT+TGG | 0.314080 | 1.2:+15304620 | MS.gene026677:CDS |
| GACATCTATTACCTCCTTGA+TGG | 0.319922 | 1.2:-15303482 | None:intergenic |
| AACGTCATAGAAATTGTTAT+TGG | 0.327718 | 1.2:-15295733 | None:intergenic |
| CTTGCCAGACTCGCCCAAAA+TGG | 0.331525 | 1.2:-15303359 | None:intergenic |
| CTTCTTCTTCAACGGTAGAT+AGG | 0.340720 | 1.2:-15295698 | None:intergenic |
| TTCTACTCTTCCTGCTCCTC+CGG | 0.342578 | 1.2:+15299109 | MS.gene026677:CDS |
| CTCCAGATCTCTTTCTGTTA+AGG | 0.350840 | 1.2:-15298965 | None:intergenic |
| TACAAAGAAACTATTCTTGT+TGG | 0.365789 | 1.2:+15301440 | MS.gene026677:CDS |
| ATAACTACTTTGAGAATAAG+AGG | 0.370258 | 1.2:-15298272 | None:intergenic |
| TTACTAAAAGATTTCTTTCA+AGG | 0.373245 | 1.2:+15301579 | MS.gene026677:CDS |
| AACTGCTAACATCTCAATAT+AGG | 0.374866 | 1.2:-15299138 | None:intergenic |
| TACTGGCAGTCTATCATTTA+CGG | 0.376132 | 1.2:+15298903 | MS.gene026677:CDS |
| TCTACTCTTCCTGCTCCTCC+GGG | 0.379705 | 1.2:+15299110 | MS.gene026677:CDS |
| CTGAGAGCATGGACTGATCT+TGG | 0.385607 | 1.2:-15298377 | None:intergenic |
| TGAGTCCTCTAATGCAATTC+CGG | 0.390906 | 1.2:+15303429 | MS.gene026677:CDS |
| ACAAAGAAACTATTCTTGTT+GGG | 0.410854 | 1.2:+15301441 | MS.gene026677:CDS |
| TGCTAGGGCTACCATGGAAT+TGG | 0.412824 | 1.2:+15301643 | MS.gene026677:CDS |
| CTTGAAACAGGACCTGGCTT+TGG | 0.421282 | 1.2:+15303301 | MS.gene026677:intron |
| GAAGAAGTCCGTGGTGGTAC+TGG | 0.424346 | 1.2:+15298886 | MS.gene026677:intron |
| AATTACGAAGAAATCTACTT+TGG | 0.438651 | 1.2:+15298559 | MS.gene026677:CDS |
| CTTGTATTGTAATCAGTGAT+AGG | 0.439169 | 1.2:-15301011 | None:intergenic |
| TAGGGAGACCCCGGAGGAGC+AGG | 0.447084 | 1.2:-15299119 | None:intergenic |
| GGACTAGTAACTTGGGTGCT+TGG | 0.448818 | 1.2:+15297198 | MS.gene026677:CDS |
| TGTCACCGGAATTGCATTAG+AGG | 0.451726 | 1.2:-15303434 | None:intergenic |
| TTCAAGGGAGATACAACAAT+CGG | 0.454495 | 1.2:+15301595 | MS.gene026677:CDS |
| ACTGCTAACATCTCAATATA+GGG | 0.455752 | 1.2:-15299137 | None:intergenic |
| TTCTTCTTCAACGGTAGATA+GGG | 0.456154 | 1.2:-15295697 | None:intergenic |
| TTGATGTGGTGCAGATTTAG+TGG | 0.456703 | 1.2:+15301225 | MS.gene026677:intron |
| GCACTATTGAAGATCAGAAA+TGG | 0.459282 | 1.2:+15301665 | MS.gene026677:CDS |
| GTGAAGGATTTATGCCATCA+TGG | 0.460569 | 1.2:+15297220 | MS.gene026677:CDS |
| ATAACTGCTGAGATGTTAGA+TGG | 0.462058 | 1.2:+15301249 | MS.gene026677:CDS |
| CAAGATCAGTCCATGCTCTC+AGG | 0.464847 | 1.2:+15298378 | MS.gene026677:CDS |
| GATGGAGCGGCGCCAAAGCC+AGG | 0.465245 | 1.2:-15303313 | None:intergenic |
| TACCTTAACAGAAAGAGATC+TGG | 0.482351 | 1.2:+15298963 | MS.gene026677:CDS |
| TAATCAGTGATAGGATTTGA+AGG | 0.488048 | 1.2:-15301002 | None:intergenic |
| GTTTCAGTTGAAGAATGAAA+AGG | 0.488517 | 1.2:+15303873 | MS.gene026677:intron |
| ACATTCAAGGACTAGTAACT+TGG | 0.493983 | 1.2:+15297190 | MS.gene026677:CDS |
| TTCAGCAGATATGTTCGACT+TGG | 0.497666 | 1.2:-15304037 | None:intergenic |
| TATGCTCTATATACCAGGCC+TGG | 0.502117 | 1.2:+15298292 | MS.gene026677:CDS |
| ATGACAGCGTCGAAGATGCT+AGG | 0.505861 | 1.2:+15301627 | MS.gene026677:CDS |
| GAAGCACTTTGGACAGTGAC+AGG | 0.506786 | 1.2:-15298328 | None:intergenic |
| ACTATCATCATCGCATTAGT+TGG | 0.507966 | 1.2:-15304117 | None:intergenic |
| GTCAGAAGAGTTCTTCGAAA+GGG | 0.512584 | 1.2:+15304007 | MS.gene026677:CDS |
| CTATCACTGATTACAATACA+AGG | 0.517380 | 1.2:+15301012 | MS.gene026677:CDS |
| TACTAAAAGATTTCTTTCAA+GGG | 0.517855 | 1.2:+15301580 | MS.gene026677:CDS |
| TACAAAGCTGCATCCAGGCC+TGG | 0.518340 | 1.2:-15298305 | None:intergenic |
| GAAGGAAATTGTTGCTCGCA+TGG | 0.522734 | 1.2:+15304062 | MS.gene026677:CDS |
| TGAAGGATTTATGCCATCAT+GGG | 0.537047 | 1.2:+15297221 | MS.gene026677:CDS |
| ACCATCTTCTTCTTCTTCAA+CGG | 0.538723 | 1.2:-15295706 | None:intergenic |
| TGAGCTAACAAGAGTTACTC+TGG | 0.540844 | 1.2:+15300139 | MS.gene026677:CDS |
| GTAACTTGGGTGCTTGGTGA+AGG | 0.541564 | 1.2:+15297204 | MS.gene026677:CDS |
| ACATGTTTACAGGACATTCA+AGG | 0.541807 | 1.2:+15297177 | MS.gene026677:intron |
| ACAGGTACAAAGCTGCATCC+AGG | 0.548395 | 1.2:-15298310 | None:intergenic |
| ATGATAGTTTGCTCTGGTCA+TGG | 0.548778 | 1.2:+15304132 | MS.gene026677:CDS |
| TTTCTACAGACCTGAGAGCA+TGG | 0.550494 | 1.2:-15298388 | None:intergenic |
| CGAAGATGCTAGGGCTACCA+TGG | 0.551544 | 1.2:+15301637 | MS.gene026677:CDS |
| GTTAGCAGTTGACTGTGAAA+TGG | 0.551942 | 1.2:+15299151 | MS.gene026677:CDS |
| GAACATATCTGCTGAACTGA+AGG | 0.555371 | 1.2:+15304044 | MS.gene026677:CDS |
| GAGTATCCTGTTAATAAGCC+AGG | 0.557188 | 1.2:+15298994 | MS.gene026677:CDS |
| CTTCAATAGTGCCAATTCCA+TGG | 0.569103 | 1.2:-15301654 | None:intergenic |
| GAGACACGGCAGTTGTTCGC+AGG | 0.573411 | 1.2:+15304154 | MS.gene026677:CDS |
| AGGAAAATGCTTACAGAGGA+GGG | 0.577646 | 1.2:+15304605 | MS.gene026677:CDS |
| CTTCCCATAAAAGTGCTCTA+CGG | 0.586029 | 1.2:+15301552 | MS.gene026677:CDS |
| GTATAGTATGTAACAGGGAA+AGG | 0.588034 | 1.2:-15298943 | None:intergenic |
| GTTACTCTGGTGGATGTCAA+AGG | 0.588203 | 1.2:+15300152 | MS.gene026677:CDS |
| TGCATAGTGCATAACAGGCG+AGG | 0.592654 | 1.2:+15300112 | MS.gene026677:intron |
| TCAAGGGAGATACAACAATC+GGG | 0.595869 | 1.2:+15301596 | MS.gene026677:CDS |
| GAGGAAAATGCTTACAGAGG+AGG | 0.596767 | 1.2:+15304604 | MS.gene026677:CDS |
| TCAGCAGATATGTTCGACTT+GGG | 0.597195 | 1.2:-15304036 | None:intergenic |
| ACCGTTGAAGAAGAAGAAGA+TGG | 0.598458 | 1.2:+15295705 | MS.gene026677:CDS |
| ATCTCAATATAGGGAGACCC+CGG | 0.600647 | 1.2:-15299128 | None:intergenic |
| GCATAGTGCATAACAGGCGA+GGG | 0.600986 | 1.2:+15300113 | MS.gene026677:intron |
| TCTGGTGGATGTCAAAGGAC+AGG | 0.603981 | 1.2:+15300157 | MS.gene026677:CDS |
| GAGATACAACAATCGGGCGA+TGG | 0.607723 | 1.2:+15301602 | MS.gene026677:CDS |
| TGACAGCGTCGAAGATGCTA+GGG | 0.608604 | 1.2:+15301628 | MS.gene026677:CDS |
| AAGCTTAAATACAAAACTTG+CGG | 0.610067 | 1.2:+15303963 | MS.gene026677:CDS |
| CATTCAAGGACTAGTAACTT+GGG | 0.611379 | 1.2:+15297191 | MS.gene026677:CDS |
| GCGATGATGATAGTTTGCTC+TGG | 0.611534 | 1.2:+15304126 | MS.gene026677:CDS |
| CTTAACAGAAAGAGATCTGG+AGG | 0.616477 | 1.2:+15298966 | MS.gene026677:CDS |
| GCTTCTTCATCAGATGTCAC+CGG | 0.616532 | 1.2:-15303448 | None:intergenic |
| TAACTGCTGAGATGTTAGAT+GGG | 0.617285 | 1.2:+15301250 | MS.gene026677:CDS |
| ATTGAAGATATCACACGACT+TGG | 0.623770 | 1.2:+15301490 | MS.gene026677:CDS |
| GTAGTTATGCTCTATATACC+AGG | 0.626200 | 1.2:+15298287 | MS.gene026677:CDS |
| TCTACTTTGGCATCTCAACA+AGG | 0.633303 | 1.2:+15298572 | MS.gene026677:CDS |
| CAGACTCGCCCAAAATGGAA+AGG | 0.634124 | 1.2:-15303354 | None:intergenic |
| TTGCTCTGGTCATGGAGACA+CGG | 0.637845 | 1.2:+15304140 | MS.gene026677:CDS |
| TGTCAGAAGAGTTCTTCGAA+AGG | 0.639622 | 1.2:+15304006 | MS.gene026677:CDS |
| CTACTCTTCCTGCTCCTCCG+GGG | 0.661696 | 1.2:+15299111 | MS.gene026677:CDS |
| GCTAACAAGAGTTACTCTGG+TGG | 0.687125 | 1.2:+15300142 | MS.gene026677:CDS |
| ATACTTGAAGAAACAAGCTG+AGG | 0.692573 | 1.2:+15303933 | MS.gene026677:CDS |
| AACTGCTGAGATGTTAGATG+GGG | 0.702600 | 1.2:+15301251 | MS.gene026677:CDS |
| CATAGTGCATAACAGGCGAG+GGG | 0.706156 | 1.2:+15300114 | MS.gene026677:intron |
| AATTGCATTAGAGGACTCAG+AGG | 0.712900 | 1.2:-15303425 | None:intergenic |
| CTTTGTGCAGAAGAAGTCCG+TGG | 0.717437 | 1.2:+15298877 | MS.gene026677:intron |
| GTTGAGGAAAATGCTTACAG+AGG | 0.721879 | 1.2:+15304601 | MS.gene026677:CDS |
| TTAAGGTATAGTATGTAACA+GGG | 0.726120 | 1.2:-15298948 | None:intergenic |
| TCAATATAGGGAGACCCCGG+AGG | 0.728696 | 1.2:-15299125 | None:intergenic |
| ATAGACTGCCAGTACCACCA+CGG | 0.744815 | 1.2:-15298894 | None:intergenic |
| TGTGCAGAAGAAGTCCGTGG+TGG | 0.766533 | 1.2:+15298880 | MS.gene026677:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 15295669 | 15304724 | 15295669 | ID=MS.gene026677 |
| chr1.2 | mRNA | 15295669 | 15304724 | 15295669 | ID=MS.gene026677.t1;Parent=MS.gene026677 |
| chr1.2 | exon | 15295669 | 15295767 | 15295669 | ID=MS.gene026677.t1.exon1;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15295669 | 15295767 | 15295669 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15297059 | 15297112 | 15297059 | ID=MS.gene026677.t1.exon2;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15297059 | 15297112 | 15297059 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15297189 | 15297254 | 15297189 | ID=MS.gene026677.t1.exon3;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15297189 | 15297254 | 15297189 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15298266 | 15298399 | 15298266 | ID=MS.gene026677.t1.exon4;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15298266 | 15298399 | 15298266 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15298481 | 15298593 | 15298481 | ID=MS.gene026677.t1.exon5;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15298481 | 15298593 | 15298481 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15298887 | 15299015 | 15298887 | ID=MS.gene026677.t1.exon6;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15298887 | 15299015 | 15298887 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15299102 | 15299172 | 15299102 | ID=MS.gene026677.t1.exon7;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15299102 | 15299172 | 15299102 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15300119 | 15300178 | 15300119 | ID=MS.gene026677.t1.exon8;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15300119 | 15300178 | 15300119 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15300978 | 15301033 | 15300978 | ID=MS.gene026677.t1.exon9;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15300978 | 15301033 | 15300978 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15301239 | 15301299 | 15301239 | ID=MS.gene026677.t1.exon10;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15301239 | 15301299 | 15301239 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15301419 | 15301686 | 15301419 | ID=MS.gene026677.t1.exon11;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15301419 | 15301686 | 15301419 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15303311 | 15303492 | 15303311 | ID=MS.gene026677.t1.exon12;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15303311 | 15303492 | 15303311 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15303880 | 15304175 | 15303880 | ID=MS.gene026677.t1.exon13;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15303880 | 15304175 | 15303880 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| chr1.2 | exon | 15304601 | 15304724 | 15304601 | ID=MS.gene026677.t1.exon14;Parent=MS.gene026677.t1 |
| chr1.2 | CDS | 15304601 | 15304724 | 15304601 | ID=cds.MS.gene026677.t1;Parent=MS.gene026677.t1 |
| Gene Sequence |
| Protein sequence |
