AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27003


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27003.t1 MTR_1g044660 100.000 239 0 0 1 239 1 239 3.63e-177 486
MS.gene27003.t1 MTR_1g023000 99.163 239 2 0 1 239 1 239 2.37e-176 484
MS.gene27003.t1 MTR_6g052140 94.538 238 8 1 1 238 1 233 6.51e-166 457
MS.gene27003.t1 MTR_6g052220 94.538 238 8 1 1 238 1 233 6.51e-166 457
MS.gene27003.t1 MTR_1g111450 86.383 235 31 1 1 234 1 235 5.09e-150 417
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27003.t1 AT5G35530 88.841 233 26 0 1 233 1 233 2.03e-153 426
MS.gene27003.t1 AT2G31610 88.987 227 25 0 1 227 1 227 4.40e-149 416
MS.gene27003.t1 AT3G53870 92.166 217 17 0 1 217 1 217 1.47e-148 414

Find 66 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGGGTGTTCTTGGTATTA+AGG 0.259759 1.3:+2304899 MS.gene27003:intron
TTAGGACCTTGCTTTCCTTT+AGG 0.279269 1.3:-2304945 None:intergenic
GCTATTCCGGTGTTGAGGTT+AGG 0.315077 1.3:+2304278 MS.gene27003:CDS
TCCCCTGTTGTTGACCTTCT+CGG 0.322481 1.3:-2304536 None:intergenic
CTTGCTGAAGATGGCTATTC+CGG 0.326131 1.3:+2304265 MS.gene27003:CDS
CGGTGTGTTGAGGTTTGTTA+TGG 0.332791 1.3:+2304633 MS.gene27003:CDS
AAACCTCAACACACCGTAAC+AGG 0.355716 1.3:-2304626 None:intergenic
CTCATCTGGCCAACCTGTTA+AGG 0.357556 1.3:+2304747 MS.gene27003:CDS
ATAGCCATCCTTGAACTTCA+TGG 0.360301 1.3:-2304719 None:intergenic
ATGTACTCTTCCTCCTCCTT+TGG 0.368666 1.3:-2305002 None:intergenic
CTATTCCGGTGTTGAGGTTA+GGG 0.371348 1.3:+2304279 MS.gene27003:CDS
GGATGTGAGGTTATTGTTAG+TGG 0.386156 1.3:+2304670 MS.gene27003:CDS
GAGTCAATGTAATCCTTAAC+AGG 0.402767 1.3:-2304760 None:intergenic
CTCCTCCTTTGGAGTGTGAA+TGG 0.408543 1.3:-2304991 None:intergenic
ATCCTTGAACTTCATGGACT+TGG 0.424680 1.3:-2304713 None:intergenic
GTGTGAATGGTAACAATATC+AGG 0.428011 1.3:-2304978 None:intergenic
AGGTGAAGATTATGCTTGAC+TGG 0.432560 1.3:+2304919 MS.gene27003:CDS
ACCAGAGAGCTTGCTGAAGA+TGG 0.438488 1.3:+2304256 MS.gene27003:CDS
ACTCGTACCCAAGCTGTTCT+CGG 0.438888 1.3:+2304337 MS.gene27003:CDS
ATGCCGAGAAGGTCAACAAC+AGG 0.451722 1.3:+2304533 MS.gene27003:CDS
GATGGCTATATGATCTCATC+TGG 0.452022 1.3:+2304733 MS.gene27003:CDS
TCTCTTCGTTACAAGCTTCT+CGG 0.453319 1.3:+2304583 MS.gene27003:CDS
TATGATTAGGTGAGAAGGGT+AGG 0.454324 1.3:+2304443 MS.gene27003:intron
CAAACAACAGGGACTGGGAC+AGG 0.457728 1.3:-2305062 None:intergenic
AGGAGGAGGAAGAGTACATC+AGG 0.461009 1.3:+2305006 MS.gene27003:CDS
TTGAGGTTTGTTATGGAAAG+TGG 0.475116 1.3:+2304640 MS.gene27003:CDS
TATAGTTCAACACTGTTCTC+AGG 0.488325 1.3:-2304511 None:intergenic
AATTTATGATTAGGTGAGAA+GGG 0.490999 1.3:+2304439 MS.gene27003:intron
GCCATCTTCAGCAAGCTCTC+TGG 0.491889 1.3:-2304257 None:intergenic
AGCCAAGTCCATGAAGTTCA+AGG 0.492737 1.3:+2304711 MS.gene27003:CDS
TGTTATGGAAAGTGGTGCTA+AGG 0.499768 1.3:+2304648 MS.gene27003:CDS
TAGACATGTTCTTCTCAGAC+AGG 0.500852 1.3:+2304789 MS.gene27003:CDS
TTGTTGCAGTTCGTCGCTGA+CGG 0.501712 1.3:+2304202 MS.gene27003:intron
TGACCTCAGTAGTCCAGAAA+AGG 0.517945 1.3:+2304479 MS.gene27003:CDS
CTACGAACCGAGAACAGCTT+GGG 0.520711 1.3:-2304344 None:intergenic
GATGATGATCTCAGTGCGCA+TGG 0.527229 1.3:-2304308 None:intergenic
TCATTTGCAACAACAGCAGC+AGG 0.528096 1.3:-2305029 None:intergenic
TTTCTTCGCTGAATTGAATG+AGG 0.530015 1.3:+2304228 MS.gene27003:CDS
AATGGTAACAATATCAGGTA+GGG 0.532486 1.3:-2304973 None:intergenic
GATTAGGTGAGAAGGGTAGG+AGG 0.538783 1.3:+2304446 MS.gene27003:intron
ACTACGAACCGAGAACAGCT+TGG 0.552773 1.3:-2304345 None:intergenic
GCTGTTCGCAGAGCCTGTTA+CGG 0.553152 1.3:+2304613 MS.gene27003:CDS
GAGCCTGTTACGGTGTGTTG+AGG 0.556363 1.3:+2304623 MS.gene27003:CDS
ATGCTTGACTGGGATCCTAA+AGG 0.556783 1.3:+2304930 MS.gene27003:CDS
TGTTGTTGCAAATGATATTG+AGG 0.562213 1.3:+2305037 MS.gene27003:CDS
GAATTTATGATTAGGTGAGA+AGG 0.566300 1.3:+2304438 MS.gene27003:intron
TTTCTCAAACAACAGGGACT+GGG 0.568525 1.3:-2305067 None:intergenic
GTTATGGAAAGTGGTGCTAA+GGG 0.582081 1.3:+2304649 MS.gene27003:CDS
GAATGGTAACAATATCAGGT+AGG 0.585408 1.3:-2304974 None:intergenic
AGATGGCTATTCCGGTGTTG+AGG 0.587442 1.3:+2304273 MS.gene27003:CDS
CAATGTAATCCTTAACAGGT+TGG 0.596885 1.3:-2304756 None:intergenic
TGGGATCCTAAAGGAAAGCA+AGG 0.601952 1.3:+2304939 MS.gene27003:CDS
GGTGAAGATTATGCTTGACT+GGG 0.603451 1.3:+2304920 MS.gene27003:CDS
AAACAACAGGGACTGGGACA+GGG 0.607283 1.3:-2305061 None:intergenic
TGCCGAGAAGGTCAACAACA+GGG 0.610201 1.3:+2304534 MS.gene27003:CDS
TACCATTCACACTCCAAAGG+AGG 0.618529 1.3:+2304989 MS.gene27003:CDS
CATTCACACTCCAAAGGAGG+AGG 0.623248 1.3:+2304992 MS.gene27003:CDS
ATGGTAACAATATCAGGTAG+GGG 0.623679 1.3:-2304972 None:intergenic
TGTTACCATTCACACTCCAA+AGG 0.627045 1.3:+2304986 MS.gene27003:CDS
TGTTGAACTATATGCCGAGA+AGG 0.629146 1.3:+2304522 MS.gene27003:CDS
AAGTCCATGAAGTTCAAGGA+TGG 0.640546 1.3:+2304715 MS.gene27003:CDS
ATGATGATCTCAGTGCGCAT+GGG 0.667262 1.3:-2304307 None:intergenic
AAGTGGTGCTAAGGGATGTG+AGG 0.670962 1.3:+2304657 MS.gene27003:CDS
CTTCGTTACAAGCTTCTCGG+AGG 0.671532 1.3:+2304586 MS.gene27003:CDS
GCCGAGAAGGTCAACAACAG+GGG 0.676600 1.3:+2304535 MS.gene27003:CDS
AGTAACCCTAACCTCAACAC+CGG 0.681090 1.3:-2304284 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTATATGAATTTAAA+TGG + chr1.3:2304400-2304419 MS.gene27003:intron 10.0%
!! TGATTTATGAATTTATGATT+AGG + chr1.3:2304430-2304449 MS.gene27003:intron 15.0%
!!! ATTATTACCTTTATTTTGTA+GGG + chr1.3:2304882-2304901 MS.gene27003:intron 15.0%
!!! GATTATTACCTTTATTTTGT+AGG + chr1.3:2304881-2304900 MS.gene27003:intron 20.0%
! AATTTATGATTAGGTGAGAA+GGG + chr1.3:2304439-2304458 MS.gene27003:intron 25.0%
AAACCAACAAGGTTGTTATA+GGG - chr1.3:2304827-2304846 None:intergenic 30.0%
AAGAACACCCTACAAAATAA+AGG - chr1.3:2304892-2304911 None:intergenic 30.0%
AATAACAAACCAAACCAACA+AGG - chr1.3:2304838-2304857 None:intergenic 30.0%
AATGGTAACAATATCAGGTA+GGG - chr1.3:2304976-2304995 None:intergenic 30.0%
CACTACTAATAGAGATGAAT+TGG - chr1.3:2304141-2304160 None:intergenic 30.0%
GAATTTATGATTAGGTGAGA+AGG + chr1.3:2304438-2304457 MS.gene27003:intron 30.0%
TGTTGTTGCAAATGATATTG+AGG + chr1.3:2305037-2305056 MS.gene27003:CDS 30.0%
!!! TTTATTTTGTAGGGTGTTCT+TGG + chr1.3:2304891-2304910 MS.gene27003:intron 30.0%
ATGGTAACAATATCAGGTAG+GGG - chr1.3:2304975-2304994 None:intergenic 35.0%
CAAACCAACAAGGTTGTTAT+AGG - chr1.3:2304828-2304847 None:intergenic 35.0%
CAATGTAATCCTTAACAGGT+TGG - chr1.3:2304759-2304778 None:intergenic 35.0%
GAATGGTAACAATATCAGGT+AGG - chr1.3:2304977-2304996 None:intergenic 35.0%
GAGTCAATGTAATCCTTAAC+AGG - chr1.3:2304763-2304782 None:intergenic 35.0%
GTGTGAATGGTAACAATATC+AGG - chr1.3:2304981-2305000 None:intergenic 35.0%
TATAGTTCAACACTGTTCTC+AGG - chr1.3:2304514-2304533 None:intergenic 35.0%
TTACCCTATAACAACCTTGT+TGG + chr1.3:2304821-2304840 MS.gene27003:intron 35.0%
TTTCTTCGCTGAATTGAATG+AGG + chr1.3:2304228-2304247 MS.gene27003:CDS 35.0%
! CAGGAAACTTAAACCTTTTC+TGG - chr1.3:2304495-2304514 None:intergenic 35.0%
! CTATAACAACCTTGTTGGTT+TGG + chr1.3:2304826-2304845 MS.gene27003:intron 35.0%
!! TTGAGGTTTGTTATGGAAAG+TGG + chr1.3:2304640-2304659 MS.gene27003:CDS 35.0%
!!! CTTTCGCTTTTTGCTCATTT+GGG - chr1.3:2304090-2304109 None:intergenic 35.0%
AAGTCCATGAAGTTCAAGGA+TGG + chr1.3:2304715-2304734 MS.gene27003:CDS 40.0%
AGGTGAAGATTATGCTTGAC+TGG + chr1.3:2304919-2304938 MS.gene27003:CDS 40.0%
ATAGCCATCCTTGAACTTCA+TGG - chr1.3:2304722-2304741 None:intergenic 40.0%
ATCCTTGAACTTCATGGACT+TGG - chr1.3:2304716-2304735 None:intergenic 40.0%
CCAAATGAGCAAAAAGCGAA+AGG + chr1.3:2304088-2304107 MS.gene27003:CDS 40.0%
GATGGCTATATGATCTCATC+TGG + chr1.3:2304733-2304752 MS.gene27003:CDS 40.0%
GGATGTGAGGTTATTGTTAG+TGG + chr1.3:2304670-2304689 MS.gene27003:CDS 40.0%
GGTGAAGATTATGCTTGACT+GGG + chr1.3:2304920-2304939 MS.gene27003:CDS 40.0%
TAGACATGTTCTTCTCAGAC+AGG + chr1.3:2304789-2304808 MS.gene27003:CDS 40.0%
TATGATTAGGTGAGAAGGGT+AGG + chr1.3:2304443-2304462 MS.gene27003:intron 40.0%
TCTCTTCGTTACAAGCTTCT+CGG + chr1.3:2304583-2304602 MS.gene27003:CDS 40.0%
TGTTACCATTCACACTCCAA+AGG + chr1.3:2304986-2305005 MS.gene27003:CDS 40.0%
TGTTGAACTATATGCCGAGA+AGG + chr1.3:2304522-2304541 MS.gene27003:CDS 40.0%
TTAGGACCTTGCTTTCCTTT+AGG - chr1.3:2304948-2304967 None:intergenic 40.0%
! AAACCTTTTCTGGACTACTG+AGG - chr1.3:2304485-2304504 None:intergenic 40.0%
! ATATCAGGTAGGGGAGTTTT+AGG - chr1.3:2304966-2304985 None:intergenic 40.0%
! GTAGGGTGTTCTTGGTATTA+AGG + chr1.3:2304899-2304918 MS.gene27003:intron 40.0%
! GTTATGGAAAGTGGTGCTAA+GGG + chr1.3:2304649-2304668 MS.gene27003:CDS 40.0%
! TGTTATGGAAAGTGGTGCTA+AGG + chr1.3:2304648-2304667 MS.gene27003:CDS 40.0%
!!! CCTTTCGCTTTTTGCTCATT+TGG - chr1.3:2304091-2304110 None:intergenic 40.0%
AAACCTCAACACACCGTAAC+AGG - chr1.3:2304629-2304648 None:intergenic 45.0%
AGCCAAGTCCATGAAGTTCA+AGG + chr1.3:2304711-2304730 MS.gene27003:CDS 45.0%
AGTAACCCTAACCTCAACAC+CGG - chr1.3:2304287-2304306 None:intergenic 45.0%
ATGATGATCTCAGTGCGCAT+GGG - chr1.3:2304310-2304329 None:intergenic 45.0%
ATGCTTGACTGGGATCCTAA+AGG + chr1.3:2304930-2304949 MS.gene27003:CDS 45.0%
ATGTACTCTTCCTCCTCCTT+TGG - chr1.3:2305005-2305024 None:intergenic 45.0%
CTTGCTGAAGATGGCTATTC+CGG + chr1.3:2304265-2304284 MS.gene27003:CDS 45.0%
TACCATTCACACTCCAAAGG+AGG + chr1.3:2304989-2305008 MS.gene27003:CDS 45.0%
TCATTTGCAACAACAGCAGC+AGG - chr1.3:2305032-2305051 None:intergenic 45.0%
TGACCTCAGTAGTCCAGAAA+AGG + chr1.3:2304479-2304498 MS.gene27003:CDS 45.0%
TGGGATCCTAAAGGAAAGCA+AGG + chr1.3:2304939-2304958 MS.gene27003:CDS 45.0%
! CTATTCCGGTGTTGAGGTTA+GGG + chr1.3:2304279-2304298 MS.gene27003:CDS 45.0%
!! CGGTGTGTTGAGGTTTGTTA+TGG + chr1.3:2304633-2304652 MS.gene27003:CDS 45.0%
AAACAACAGGGACTGGGACA+GGG - chr1.3:2305064-2305083 None:intergenic 50.0%
ACTACGAACCGAGAACAGCT+TGG - chr1.3:2304348-2304367 None:intergenic 50.0%
ACTCGTACCCAAGCTGTTCT+CGG + chr1.3:2304337-2304356 MS.gene27003:CDS 50.0%
ATGCCGAGAAGGTCAACAAC+AGG + chr1.3:2304533-2304552 MS.gene27003:CDS 50.0%
CATTCACACTCCAAAGGAGG+AGG + chr1.3:2304992-2305011 MS.gene27003:CDS 50.0%
CTACGAACCGAGAACAGCTT+GGG - chr1.3:2304347-2304366 None:intergenic 50.0%
CTCATCTGGCCAACCTGTTA+AGG + chr1.3:2304747-2304766 MS.gene27003:CDS 50.0%
CTTCGTTACAAGCTTCTCGG+AGG + chr1.3:2304586-2304605 MS.gene27003:CDS 50.0%
GATGATGATCTCAGTGCGCA+TGG - chr1.3:2304311-2304330 None:intergenic 50.0%
GATTAGGTGAGAAGGGTAGG+AGG + chr1.3:2304446-2304465 MS.gene27003:intron 50.0%
TCCCCTGTTGTTGACCTTCT+CGG - chr1.3:2304539-2304558 None:intergenic 50.0%
TGCCGAGAAGGTCAACAACA+GGG + chr1.3:2304534-2304553 MS.gene27003:CDS 50.0%
TTGTTGCAGTTCGTCGCTGA+CGG + chr1.3:2304202-2304221 MS.gene27003:intron 50.0%
! AAGTGGTGCTAAGGGATGTG+AGG + chr1.3:2304657-2304676 MS.gene27003:CDS 50.0%
! AGATGGCTATTCCGGTGTTG+AGG + chr1.3:2304273-2304292 MS.gene27003:CDS 50.0%
! AGGAGGAGGAAGAGTACATC+AGG + chr1.3:2305006-2305025 MS.gene27003:CDS 50.0%
! GCTATTCCGGTGTTGAGGTT+AGG + chr1.3:2304278-2304297 MS.gene27003:CDS 50.0%
!! ACCAGAGAGCTTGCTGAAGA+TGG + chr1.3:2304256-2304275 MS.gene27003:CDS 50.0%
!! CTCCTCCTTTGGAGTGTGAA+TGG - chr1.3:2304994-2305013 None:intergenic 50.0%
CAAACAACAGGGACTGGGAC+AGG - chr1.3:2305065-2305084 None:intergenic 55.0%
GAGCCTGTTACGGTGTGTTG+AGG + chr1.3:2304623-2304642 MS.gene27003:CDS 55.0%
GCCATCTTCAGCAAGCTCTC+TGG - chr1.3:2304260-2304279 None:intergenic 55.0%
GCCGAGAAGGTCAACAACAG+GGG + chr1.3:2304535-2304554 MS.gene27003:CDS 55.0%
GCTGTTCGCAGAGCCTGTTA+CGG + chr1.3:2304613-2304632 MS.gene27003:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 2304080 2305085 2304080 ID=MS.gene27003
chr1.3 mRNA 2304080 2305085 2304080 ID=MS.gene27003.t1;Parent=MS.gene27003
chr1.3 exon 2304080 2304109 2304080 ID=MS.gene27003.t1.exon1;Parent=MS.gene27003.t1
chr1.3 CDS 2304080 2304109 2304080 ID=cds.MS.gene27003.t1;Parent=MS.gene27003.t1
chr1.3 exon 2304211 2304358 2304211 ID=MS.gene27003.t1.exon2;Parent=MS.gene27003.t1
chr1.3 CDS 2304211 2304358 2304211 ID=cds.MS.gene27003.t1;Parent=MS.gene27003.t1
chr1.3 exon 2304452 2304810 2304452 ID=MS.gene27003.t1.exon3;Parent=MS.gene27003.t1
chr1.3 CDS 2304452 2304810 2304452 ID=cds.MS.gene27003.t1;Parent=MS.gene27003.t1
chr1.3 exon 2304903 2305085 2304903 ID=MS.gene27003.t1.exon4;Parent=MS.gene27003.t1
chr1.3 CDS 2304903 2305085 2304903 ID=cds.MS.gene27003.t1;Parent=MS.gene27003.t1
Gene Sequence

>MS.gene27003

ATGGCTACCCAAATGAGCAAAAAGCGAAAGGTTTGTTTTCAGATTCGTTAATCTTAATCCAATTCATCTCTATTAGTAGTGATTTCTTAATTCTTCTTAATTTAACAATTTGATTTGTGTTGTTGTTGCAGTTCGTCGCTGACGGAGTTTTCTTCGCTGAATTGAATGAGGTTTTAACCAGAGAGCTTGCTGAAGATGGCTATTCCGGTGTTGAGGTTAGGGTTACTCCCATGCGCACTGAGATCATCATCAGAGCTACTCGTACCCAAGCTGTTCTCGGTTCGTAGTCATTAATTTACAAATCTGATTTTATTTGTTTTTGTTTTTATATGAATTTAAATGGTTGAATCTGATTTATGAATTTATGATTAGGTGAGAAGGGTAGGAGGATCAGAGAACTGACCTCAGTAGTCCAGAAAAGGTTTAAGTTTCCTGAGAACAGTGTTGAACTATATGCCGAGAAGGTCAACAACAGGGGACTTTGTGCAATTGCACAAGCTGAGTCTCTTCGTTACAAGCTTCTCGGAGGTCTTGCTGTTCGCAGAGCCTGTTACGGTGTGTTGAGGTTTGTTATGGAAAGTGGTGCTAAGGGATGTGAGGTTATTGTTAGTGGAAAATTGAGAGCACAAAGAGCCAAGTCCATGAAGTTCAAGGATGGCTATATGATCTCATCTGGCCAACCTGTTAAGGATTACATTGACTCTGCTGTTAGACATGTTCTTCTCAGACAGGTTAGTTAGTTTACCCTATAACAACCTTGTTGGTTTGGTTTGTTATTGATTTACTTATGTTGCTAATGATGATTATTACCTTTATTTTGTAGGGTGTTCTTGGTATTAAGGTGAAGATTATGCTTGACTGGGATCCTAAAGGAAAGCAAGGTCCTAAAACTCCCCTACCTGATATTGTTACCATTCACACTCCAAAGGAGGAGGAAGAGTACATCAGGCCTGCTGCTGTTGTTGCAAATGATATTGAGGTCCCTGTCCCAGTCCCTGTTGTTTGA

Protein sequence

>MS.gene27003.t1

MATQMSKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQAVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMISSGQPVKDYIDSAVRHVLLRQGVLGIKVKIMLDWDPKGKQGPKTPLPDIVTIHTPKEEEEYIRPAAVVANDIEVPVPVPVV