AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90723


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90723.t1 MTR_1g044660 100.000 239 0 0 1 239 1 239 3.63e-177 486
MS.gene90723.t1 MTR_1g023000 99.163 239 2 0 1 239 1 239 2.37e-176 484
MS.gene90723.t1 MTR_6g052140 94.538 238 8 1 1 238 1 233 6.51e-166 457
MS.gene90723.t1 MTR_6g052220 94.538 238 8 1 1 238 1 233 6.51e-166 457
MS.gene90723.t1 MTR_1g111450 86.383 235 31 1 1 234 1 235 5.09e-150 417
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90723.t1 AT5G35530 88.841 233 26 0 1 233 1 233 2.03e-153 426
MS.gene90723.t1 AT2G31610 88.987 227 25 0 1 227 1 227 4.40e-149 416
MS.gene90723.t1 AT3G53870 92.166 217 17 0 1 217 1 217 1.47e-148 414

Find 66 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGGGTGTTCTTGGTATTA+AGG 0.259759 1.4:+1593738 MS.gene90723:intron
TTAGGACCTTGCTTTCCTTT+AGG 0.279269 1.4:-1593784 None:intergenic
GCTATTCCGGTGTTGAGGTT+AGG 0.315077 1.4:+1593113 MS.gene90723:CDS
CTTGCTGAAGATGGCTATTC+CGG 0.326131 1.4:+1593100 MS.gene90723:CDS
TCCCCTGTTGTTAACCTTCT+CGG 0.326817 1.4:-1593375 None:intergenic
CGGTGTGTTGAGGTTTGTTA+TGG 0.332791 1.4:+1593472 MS.gene90723:CDS
AAACCTCAACACACCGTAAC+AGG 0.355716 1.4:-1593465 None:intergenic
CTCATCTGGCCAACCTGTTA+AGG 0.357556 1.4:+1593586 MS.gene90723:CDS
ATGTACTCTTCCTCCTCCTT+TGG 0.368666 1.4:-1593841 None:intergenic
GTAGCCATCCTTGAACTTCA+TGG 0.370346 1.4:-1593558 None:intergenic
CTATTCCGGTGTTGAGGTTA+GGG 0.371348 1.4:+1593114 MS.gene90723:CDS
GGATGTGAGGTTATTGTTAG+TGG 0.386156 1.4:+1593509 MS.gene90723:CDS
GAGTCAATGTAATCCTTAAC+AGG 0.402767 1.4:-1593599 None:intergenic
CTCCTCCTTTGGAGTGTGAA+TGG 0.408543 1.4:-1593830 None:intergenic
ATCCTTGAACTTCATGGACT+TGG 0.424680 1.4:-1593552 None:intergenic
GTGTGAATGGTAACAATATC+AGG 0.428011 1.4:-1593817 None:intergenic
AGGTGAAGATTATGCTTGAC+TGG 0.432560 1.4:+1593758 MS.gene90723:CDS
ACCAGAGAGCTTGCTGAAGA+TGG 0.438488 1.4:+1593091 MS.gene90723:CDS
ACTCGTACCCAAGCTGTTCT+CGG 0.438888 1.4:+1593172 MS.gene90723:CDS
TCTCTTCGTTACAAGCTTCT+CGG 0.453319 1.4:+1593422 MS.gene90723:CDS
ATGCCGAGAAGGTTAACAAC+AGG 0.456910 1.4:+1593372 MS.gene90723:CDS
CAAACAACAGGGACTGGGAC+AGG 0.457728 1.4:-1593901 None:intergenic
AGGAGGAGGAAGAGTACATC+AGG 0.461009 1.4:+1593845 MS.gene90723:CDS
GATGGCTACATGATCTCATC+TGG 0.466529 1.4:+1593572 MS.gene90723:CDS
TTGAGGTTTGTTATGGAAAG+TGG 0.475116 1.4:+1593479 MS.gene90723:CDS
GATTAGGTGAGAAGGGAAGG+AGG 0.484267 1.4:+1593285 MS.gene90723:intron
TATAGTTCAACACTGTTCTC+AGG 0.488325 1.4:-1593350 None:intergenic
GCCATCTTCAGCAAGCTCTC+TGG 0.491889 1.4:-1593092 None:intergenic
AGCCAAGTCCATGAAGTTCA+AGG 0.492737 1.4:+1593550 MS.gene90723:CDS
AATTAATGATTAGGTGAGAA+GGG 0.493314 1.4:+1593278 MS.gene90723:intron
TGTTATGGAAAGTGGTGCTA+AGG 0.499768 1.4:+1593487 MS.gene90723:CDS
TAGACATGTTCTTCTCAGAC+AGG 0.500852 1.4:+1593628 MS.gene90723:CDS
TTGTTGCAGTTCGTCGCTGA+CGG 0.501712 1.4:+1593037 MS.gene90723:intron
AATGATTAGGTGAGAAGGGA+AGG 0.513264 1.4:+1593282 MS.gene90723:intron
TGACCTCAGTAGTCCAGAAA+AGG 0.517945 1.4:+1593318 MS.gene90723:CDS
CTACGAACCGAGAACAGCTT+GGG 0.520711 1.4:-1593179 None:intergenic
GATGATGATCTCAGTGCGCA+TGG 0.527229 1.4:-1593143 None:intergenic
TCATTTGCAACAACAGCAGC+AGG 0.528096 1.4:-1593868 None:intergenic
TTTCTTCGCTGAATTGAATG+AGG 0.530015 1.4:+1593063 MS.gene90723:CDS
AATGGTAACAATATCAGGTA+GGG 0.532486 1.4:-1593812 None:intergenic
ACTACGAACCGAGAACAGCT+TGG 0.552773 1.4:-1593180 None:intergenic
GAGCCTGTTACGGTGTGTTG+AGG 0.556363 1.4:+1593462 MS.gene90723:CDS
ATGCTTGACTGGGATCCTAA+AGG 0.556783 1.4:+1593769 MS.gene90723:CDS
TGTTGTTGCAAATGATATTG+AGG 0.562213 1.4:+1593876 MS.gene90723:CDS
TTTCTCAAACAACAGGGACT+GGG 0.568525 1.4:-1593906 None:intergenic
GTTATGGAAAGTGGTGCTAA+GGG 0.582081 1.4:+1593488 MS.gene90723:CDS
GAATGGTAACAATATCAGGT+AGG 0.585408 1.4:-1593813 None:intergenic
AGATGGCTATTCCGGTGTTG+AGG 0.587442 1.4:+1593108 MS.gene90723:CDS
GAATTAATGATTAGGTGAGA+AGG 0.589593 1.4:+1593277 MS.gene90723:intron
GCTGTACGCAGAGCCTGTTA+CGG 0.590235 1.4:+1593452 MS.gene90723:CDS
CAATGTAATCCTTAACAGGT+TGG 0.596885 1.4:-1593595 None:intergenic
TGGGATCCTAAAGGAAAGCA+AGG 0.601952 1.4:+1593778 MS.gene90723:CDS
GGTGAAGATTATGCTTGACT+GGG 0.603451 1.4:+1593759 MS.gene90723:CDS
AAACAACAGGGACTGGGACA+GGG 0.607283 1.4:-1593900 None:intergenic
TGTTGAACTATATGCCGAGA+AGG 0.615665 1.4:+1593361 MS.gene90723:CDS
TACCATTCACACTCCAAAGG+AGG 0.618529 1.4:+1593828 MS.gene90723:CDS
CATTCACACTCCAAAGGAGG+AGG 0.623248 1.4:+1593831 MS.gene90723:CDS
ATGGTAACAATATCAGGTAG+GGG 0.623679 1.4:-1593811 None:intergenic
TGTTACCATTCACACTCCAA+AGG 0.627045 1.4:+1593825 MS.gene90723:CDS
AAGTCCATGAAGTTCAAGGA+TGG 0.640546 1.4:+1593554 MS.gene90723:CDS
ATGATGATCTCAGTGCGCAT+GGG 0.667262 1.4:-1593142 None:intergenic
AAGTGGTGCTAAGGGATGTG+AGG 0.670962 1.4:+1593496 MS.gene90723:CDS
CTTCGTTACAAGCTTCTCGG+AGG 0.671532 1.4:+1593425 MS.gene90723:CDS
TGCCGAGAAGGTTAACAACA+GGG 0.674234 1.4:+1593373 MS.gene90723:CDS
AGTAACCCTAACCTCAACAC+CGG 0.681090 1.4:-1593119 None:intergenic
GCCGAGAAGGTTAACAACAG+GGG 0.682645 1.4:+1593374 MS.gene90723:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTATATGAATTTAAA+TGG + chr1.4:1593239-1593258 MS.gene90723:intron 10.0%
!! TGATTTATGAATTAATGATT+AGG + chr1.4:1593269-1593288 MS.gene90723:intron 15.0%
!!! ATTATTACCTTTATTTTGTA+GGG + chr1.4:1593721-1593740 MS.gene90723:intron 15.0%
!!! GATTATTACCTTTATTTTGT+AGG + chr1.4:1593720-1593739 MS.gene90723:intron 20.0%
! AATTAATGATTAGGTGAGAA+GGG + chr1.4:1593278-1593297 MS.gene90723:intron 25.0%
AAACCAACAAGGTTGTTATA+GGG - chr1.4:1593666-1593685 None:intergenic 30.0%
AAGAACACCCTACAAAATAA+AGG - chr1.4:1593731-1593750 None:intergenic 30.0%
AATAACAAACCAAACCAACA+AGG - chr1.4:1593677-1593696 None:intergenic 30.0%
AATGGTAACAATATCAGGTA+GGG - chr1.4:1593815-1593834 None:intergenic 30.0%
CACTACTAATAGAGATGAAT+TGG - chr1.4:1592976-1592995 None:intergenic 30.0%
GAATTAATGATTAGGTGAGA+AGG + chr1.4:1593277-1593296 MS.gene90723:intron 30.0%
TGTTGTTGCAAATGATATTG+AGG + chr1.4:1593876-1593895 MS.gene90723:CDS 30.0%
!!! TTTATTTTGTAGGGTGTTCT+TGG + chr1.4:1593730-1593749 MS.gene90723:intron 30.0%
ATGGTAACAATATCAGGTAG+GGG - chr1.4:1593814-1593833 None:intergenic 35.0%
CAAACCAACAAGGTTGTTAT+AGG - chr1.4:1593667-1593686 None:intergenic 35.0%
CAATGTAATCCTTAACAGGT+TGG - chr1.4:1593598-1593617 None:intergenic 35.0%
GAATGGTAACAATATCAGGT+AGG - chr1.4:1593816-1593835 None:intergenic 35.0%
GAGTCAATGTAATCCTTAAC+AGG - chr1.4:1593602-1593621 None:intergenic 35.0%
GTGTGAATGGTAACAATATC+AGG - chr1.4:1593820-1593839 None:intergenic 35.0%
TATAGTTCAACACTGTTCTC+AGG - chr1.4:1593353-1593372 None:intergenic 35.0%
TTACCCTATAACAACCTTGT+TGG + chr1.4:1593660-1593679 MS.gene90723:intron 35.0%
TTTCTTCGCTGAATTGAATG+AGG + chr1.4:1593063-1593082 MS.gene90723:CDS 35.0%
! CAGGAAACTTAAACCTTTTC+TGG - chr1.4:1593334-1593353 None:intergenic 35.0%
! CTATAACAACCTTGTTGGTT+TGG + chr1.4:1593665-1593684 MS.gene90723:intron 35.0%
!! TTGAGGTTTGTTATGGAAAG+TGG + chr1.4:1593479-1593498 MS.gene90723:CDS 35.0%
!!! CTTTCGCTTTTTGCTCATTT+GGG - chr1.4:1592925-1592944 None:intergenic 35.0%
AAGTCCATGAAGTTCAAGGA+TGG + chr1.4:1593554-1593573 MS.gene90723:CDS 40.0%
AATGATTAGGTGAGAAGGGA+AGG + chr1.4:1593282-1593301 MS.gene90723:intron 40.0%
AGGTGAAGATTATGCTTGAC+TGG + chr1.4:1593758-1593777 MS.gene90723:CDS 40.0%
ATCCTTGAACTTCATGGACT+TGG - chr1.4:1593555-1593574 None:intergenic 40.0%
CCAAATGAGCAAAAAGCGAA+AGG + chr1.4:1592923-1592942 MS.gene90723:CDS 40.0%
GGATGTGAGGTTATTGTTAG+TGG + chr1.4:1593509-1593528 MS.gene90723:CDS 40.0%
GGTGAAGATTATGCTTGACT+GGG + chr1.4:1593759-1593778 MS.gene90723:CDS 40.0%
TAGACATGTTCTTCTCAGAC+AGG + chr1.4:1593628-1593647 MS.gene90723:CDS 40.0%
TCTCTTCGTTACAAGCTTCT+CGG + chr1.4:1593422-1593441 MS.gene90723:CDS 40.0%
TGTTACCATTCACACTCCAA+AGG + chr1.4:1593825-1593844 MS.gene90723:CDS 40.0%
TGTTGAACTATATGCCGAGA+AGG + chr1.4:1593361-1593380 MS.gene90723:CDS 40.0%
TTAGGACCTTGCTTTCCTTT+AGG - chr1.4:1593787-1593806 None:intergenic 40.0%
! AAACCTTTTCTGGACTACTG+AGG - chr1.4:1593324-1593343 None:intergenic 40.0%
! ATATCAGGTAGGGGAGTTTT+AGG - chr1.4:1593805-1593824 None:intergenic 40.0%
! GTAGGGTGTTCTTGGTATTA+AGG + chr1.4:1593738-1593757 MS.gene90723:intron 40.0%
! GTTATGGAAAGTGGTGCTAA+GGG + chr1.4:1593488-1593507 MS.gene90723:CDS 40.0%
! TGTTATGGAAAGTGGTGCTA+AGG + chr1.4:1593487-1593506 MS.gene90723:CDS 40.0%
!!! CCTTTCGCTTTTTGCTCATT+TGG - chr1.4:1592926-1592945 None:intergenic 40.0%
AAACCTCAACACACCGTAAC+AGG - chr1.4:1593468-1593487 None:intergenic 45.0%
AGCCAAGTCCATGAAGTTCA+AGG + chr1.4:1593550-1593569 MS.gene90723:CDS 45.0%
AGTAACCCTAACCTCAACAC+CGG - chr1.4:1593122-1593141 None:intergenic 45.0%
ATGATGATCTCAGTGCGCAT+GGG - chr1.4:1593145-1593164 None:intergenic 45.0%
ATGCCGAGAAGGTTAACAAC+AGG + chr1.4:1593372-1593391 MS.gene90723:CDS 45.0%
ATGCTTGACTGGGATCCTAA+AGG + chr1.4:1593769-1593788 MS.gene90723:CDS 45.0%
ATGTACTCTTCCTCCTCCTT+TGG - chr1.4:1593844-1593863 None:intergenic 45.0%
CTTGCTGAAGATGGCTATTC+CGG + chr1.4:1593100-1593119 MS.gene90723:CDS 45.0%
GATGGCTACATGATCTCATC+TGG + chr1.4:1593572-1593591 MS.gene90723:CDS 45.0%
GTAGCCATCCTTGAACTTCA+TGG - chr1.4:1593561-1593580 None:intergenic 45.0%
TACCATTCACACTCCAAAGG+AGG + chr1.4:1593828-1593847 MS.gene90723:CDS 45.0%
TCATTTGCAACAACAGCAGC+AGG - chr1.4:1593871-1593890 None:intergenic 45.0%
TCCCCTGTTGTTAACCTTCT+CGG - chr1.4:1593378-1593397 None:intergenic 45.0%
TGACCTCAGTAGTCCAGAAA+AGG + chr1.4:1593318-1593337 MS.gene90723:CDS 45.0%
TGCCGAGAAGGTTAACAACA+GGG + chr1.4:1593373-1593392 MS.gene90723:CDS 45.0%
TGGGATCCTAAAGGAAAGCA+AGG + chr1.4:1593778-1593797 MS.gene90723:CDS 45.0%
! CTATTCCGGTGTTGAGGTTA+GGG + chr1.4:1593114-1593133 MS.gene90723:CDS 45.0%
!! CGGTGTGTTGAGGTTTGTTA+TGG + chr1.4:1593472-1593491 MS.gene90723:CDS 45.0%
AAACAACAGGGACTGGGACA+GGG - chr1.4:1593903-1593922 None:intergenic 50.0%
ACTACGAACCGAGAACAGCT+TGG - chr1.4:1593183-1593202 None:intergenic 50.0%
ACTCGTACCCAAGCTGTTCT+CGG + chr1.4:1593172-1593191 MS.gene90723:CDS 50.0%
CATTCACACTCCAAAGGAGG+AGG + chr1.4:1593831-1593850 MS.gene90723:CDS 50.0%
CTACGAACCGAGAACAGCTT+GGG - chr1.4:1593182-1593201 None:intergenic 50.0%
CTCATCTGGCCAACCTGTTA+AGG + chr1.4:1593586-1593605 MS.gene90723:CDS 50.0%
CTTCGTTACAAGCTTCTCGG+AGG + chr1.4:1593425-1593444 MS.gene90723:CDS 50.0%
GATGATGATCTCAGTGCGCA+TGG - chr1.4:1593146-1593165 None:intergenic 50.0%
GATTAGGTGAGAAGGGAAGG+AGG + chr1.4:1593285-1593304 MS.gene90723:intron 50.0%
GCCGAGAAGGTTAACAACAG+GGG + chr1.4:1593374-1593393 MS.gene90723:CDS 50.0%
TTGTTGCAGTTCGTCGCTGA+CGG + chr1.4:1593037-1593056 MS.gene90723:intron 50.0%
! AAGTGGTGCTAAGGGATGTG+AGG + chr1.4:1593496-1593515 MS.gene90723:CDS 50.0%
! AGATGGCTATTCCGGTGTTG+AGG + chr1.4:1593108-1593127 MS.gene90723:CDS 50.0%
! AGGAGGAGGAAGAGTACATC+AGG + chr1.4:1593845-1593864 MS.gene90723:CDS 50.0%
! GCTATTCCGGTGTTGAGGTT+AGG + chr1.4:1593113-1593132 MS.gene90723:CDS 50.0%
!! ACCAGAGAGCTTGCTGAAGA+TGG + chr1.4:1593091-1593110 MS.gene90723:CDS 50.0%
!! CTCCTCCTTTGGAGTGTGAA+TGG - chr1.4:1593833-1593852 None:intergenic 50.0%
CAAACAACAGGGACTGGGAC+AGG - chr1.4:1593904-1593923 None:intergenic 55.0%
GAGCCTGTTACGGTGTGTTG+AGG + chr1.4:1593462-1593481 MS.gene90723:CDS 55.0%
GCCATCTTCAGCAAGCTCTC+TGG - chr1.4:1593095-1593114 None:intergenic 55.0%
GCTGTACGCAGAGCCTGTTA+CGG + chr1.4:1593452-1593471 MS.gene90723:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 1592915 1593924 1592915 ID=MS.gene90723
chr1.4 mRNA 1592915 1593924 1592915 ID=MS.gene90723.t1;Parent=MS.gene90723
chr1.4 exon 1592915 1592944 1592915 ID=MS.gene90723.t1.exon1;Parent=MS.gene90723.t1
chr1.4 CDS 1592915 1592944 1592915 ID=cds.MS.gene90723.t1;Parent=MS.gene90723.t1
chr1.4 exon 1593046 1593193 1593046 ID=MS.gene90723.t1.exon2;Parent=MS.gene90723.t1
chr1.4 CDS 1593046 1593193 1593046 ID=cds.MS.gene90723.t1;Parent=MS.gene90723.t1
chr1.4 exon 1593291 1593649 1593291 ID=MS.gene90723.t1.exon3;Parent=MS.gene90723.t1
chr1.4 CDS 1593291 1593649 1593291 ID=cds.MS.gene90723.t1;Parent=MS.gene90723.t1
chr1.4 exon 1593742 1593924 1593742 ID=MS.gene90723.t1.exon4;Parent=MS.gene90723.t1
chr1.4 CDS 1593742 1593924 1593742 ID=cds.MS.gene90723.t1;Parent=MS.gene90723.t1
Gene Sequence

>MS.gene90723

ATGGCTACCCAAATGAGCAAAAAGCGAAAGGTTCGTTCTCAGATTCGTTAATCGTAATCCAATTCATCTCTATTAGTAGTGATTTCTTAATAATTCTTAATTTGACAATTTGATTTGTGTTGTTGTTGCAGTTCGTCGCTGACGGAGTTTTCTTCGCTGAATTGAATGAGGTTTTAACCAGAGAGCTTGCTGAAGATGGCTATTCCGGTGTTGAGGTTAGGGTTACTCCCATGCGCACTGAGATCATCATCAGAGCTACTCGTACCCAAGCTGTTCTCGGTTCGTAGTCATTAATTTATTACGAATCTGATTTTATTTATTTATTTGTTTTATATGAATTTAAATGGTTGAATCTGATTTATGAATTAATGATTAGGTGAGAAGGGAAGGAGGATCAGAGAACTGACCTCAGTAGTCCAGAAAAGGTTTAAGTTTCCTGAGAACAGTGTTGAACTATATGCCGAGAAGGTTAACAACAGGGGACTTTGTGCAATTGCACAAGCTGAGTCTCTTCGTTACAAGCTTCTCGGAGGTCTTGCTGTACGCAGAGCCTGTTACGGTGTGTTGAGGTTTGTTATGGAAAGTGGTGCTAAGGGATGTGAGGTTATTGTTAGTGGAAAATTGAGAGCACAAAGAGCCAAGTCCATGAAGTTCAAGGATGGCTACATGATCTCATCTGGCCAACCTGTTAAGGATTACATTGACTCTGCTGTTAGACATGTTCTTCTCAGACAGGTTAGTTAGTTTACCCTATAACAACCTTGTTGGTTTGGTTTGTTATTGATTTATTTATGTTGCTAATGATGATTATTACCTTTATTTTGTAGGGTGTTCTTGGTATTAAGGTGAAGATTATGCTTGACTGGGATCCTAAAGGAAAGCAAGGTCCTAAAACTCCCCTACCTGATATTGTTACCATTCACACTCCAAAGGAGGAGGAAGAGTACATCAGGCCTGCTGCTGTTGTTGCAAATGATATTGAGGTCCCTGTCCCAGTCCCTGTTGTTTGA

Protein sequence

>MS.gene90723.t1

MATQMSKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQAVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMISSGQPVKDYIDSAVRHVLLRQGVLGIKVKIMLDWDPKGKQGPKTPLPDIVTIHTPKEEEEYIRPAAVVANDIEVPVPVPVV