Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63685.t1 | XP_003590123.1 | 98.3 | 229 | 4 | 0 | 1 | 229 | 11 | 239 | 3.40E-117 | 431 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63685.t1 | Q9FJA6 | 87.4 | 223 | 28 | 0 | 1 | 223 | 11 | 233 | 1.3e-104 | 380.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63685.t1 | B7FH64 | 98.3 | 229 | 4 | 0 | 1 | 229 | 11 | 239 | 2.4e-117 | 431.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene63685 | MS.gene20081 | PPI |
| MS.gene63685 | MS.gene93153 | PPI |
| MS.gene63685 | MS.gene98228 | PPI |
| MS.gene63685 | MS.gene24232 | PPI |
| MS.gene63685 | MS.gene27003 | PPI |
| MS.gene63685 | MS.gene08126 | PPI |
| MS.gene63685 | MS.gene90723 | PPI |
| MS.gene63685 | MS.gene36891 | PPI |
| MS.gene63685 | MS.gene053439 | PPI |
| MS.gene63685 | MS.gene31853 | PPI |
| MS.gene63685 | MS.gene98229 | PPI |
| MS.gene63685 | MS.gene055646 | PPI |
| MS.gene63685 | MS.gene063717 | PPI |
| MS.gene63685 | MS.gene33653 | PPI |
| MS.gene41081 | MS.gene63685 | PPI |
| MS.gene63685 | MS.gene073885 | PPI |
| MS.gene63685 | MS.gene96480 | PPI |
| MS.gene034801 | MS.gene63685 | PPI |
| MS.gene63685 | MS.gene026677 | PPI |
| MS.gene055644 | MS.gene63685 | PPI |
| MS.gene63685 | MS.gene27265 | PPI |
| MS.gene63685 | MS.gene41083 | PPI |
| MS.gene63685 | MS.gene061619 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63685.t1 | MTR_1g044660 | 98.253 | 229 | 4 | 0 | 1 | 229 | 11 | 239 | 1.25e-164 | 454 |
| MS.gene63685.t1 | MTR_1g023000 | 97.380 | 229 | 6 | 0 | 1 | 229 | 11 | 239 | 5.92e-164 | 452 |
| MS.gene63685.t1 | MTR_6g052140 | 92.544 | 228 | 12 | 1 | 1 | 228 | 11 | 233 | 1.85e-153 | 426 |
| MS.gene63685.t1 | MTR_6g052220 | 92.544 | 228 | 12 | 1 | 1 | 228 | 11 | 233 | 1.85e-153 | 426 |
| MS.gene63685.t1 | MTR_1g111450 | 84.444 | 225 | 34 | 1 | 2 | 225 | 12 | 236 | 1.92e-139 | 390 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63685.t1 | AT5G35530 | 87.444 | 223 | 28 | 0 | 1 | 223 | 11 | 233 | 3.84e-143 | 400 |
| MS.gene63685.t1 | AT3G53870 | 91.304 | 207 | 18 | 0 | 1 | 207 | 11 | 217 | 5.23e-139 | 389 |
| MS.gene63685.t1 | AT2G31610 | 87.558 | 217 | 27 | 0 | 1 | 217 | 11 | 227 | 5.66e-139 | 389 |
Find 0 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGATTTATGAATTTATGATT+AGG | + | 57522:3947-3966 | MS.gene63685:intron | 15.0% |
| !! | AAATCAATGAAATTTAAGGA+CGG | + | 57522:4232-4251 | MS.gene63685:CDS | 20.0% |
| !! | AGCTAAATCAATGAAATTTA+AGG | + | 57522:4228-4247 | MS.gene63685:CDS | 20.0% |
| !!! | ATTATGTCATTTATGTTGTA+GGG | + | 57522:4399-4418 | MS.gene63685:intron | 20.0% |
| ! | AATCAATGAAATTTAAGGAC+GGG | + | 57522:4233-4252 | MS.gene63685:CDS | 25.0% |
| ! | TATTGTCAGTGTAAAATTGA+GGG | + | 57522:4198-4217 | MS.gene63685:CDS | 25.0% |
| ! | TTATTGTCAGTGTAAAATTG+AGG | + | 57522:4197-4216 | MS.gene63685:CDS | 25.0% |
| !! | GATTATGTCATTTATGTTGT+AGG | + | 57522:4398-4417 | MS.gene63685:intron | 25.0% |
| AATGGTAACAATATCAGGTA+GGG | - | 57522:4493-4512 | None:intergenic | 30.0% | |
| TATGATTAGGTGAAAAGAGT+AGG | + | 57522:3960-3979 | MS.gene63685:intron | 30.0% | |
| TTATGATCCAACAACCTTAT+TGG | + | 57522:4334-4353 | MS.gene63685:intron | 30.0% | |
| ! | CAGGAAACTTAAATCTTTTC+TGG | - | 57522:4012-4031 | MS.gene63685:intergenic | 30.0% |
| !! | TTAAGGTTTGTTATGGAAAG+TGG | + | 57522:4157-4176 | MS.gene63685:CDS | 30.0% |
| !! | TTCGTTCATATGGTTTGTTA+TGG | + | 57522:4360-4379 | MS.gene63685:intron | 30.0% |
| AGGTAAAGATTATGCTTGAC+TGG | + | 57522:4436-4455 | MS.gene63685:CDS | 35.0% | |
| ATATGAACGAACGACCAATA+AGG | - | 57522:4351-4370 | MS.gene63685:intergenic | 35.0% | |
| ATGGTAACAATATCAGGTAG+GGG | - | 57522:4492-4511 | MS.gene63685:intergenic | 35.0% | |
| GAATGGTAACAATATCAGGT+AGG | - | 57522:4494-4513 | MS.gene63685:intergenic | 35.0% | |
| GAGTCAATGTAATCCTTAAC+AGG | - | 57522:4280-4299 | MS.gene63685:intergenic | 35.0% | |
| GGTAAAGATTATGCTTGACT+GGG | + | 57522:4437-4456 | MS.gene63685:CDS | 35.0% | |
| GTGTGAATGGTAACAATATC+AGG | - | 57522:4498-4517 | MS.gene63685:intergenic | 35.0% | |
| TAGACATGTTCTTCTTAGAC+AGG | + | 57522:4306-4325 | MS.gene63685:CDS | 35.0% | |
| TCTCTTCTTTACAAGCTTCT+CGG | + | 57522:4100-4119 | MS.gene63685:CDS | 35.0% | |
| TGTTGTTGCAAATGATATCG+AGG | + | 57522:4554-4573 | MS.gene63685:CDS | 35.0% | |
| TTATTGGTCGTTCGTTCATA+TGG | + | 57522:4350-4369 | MS.gene63685:intron | 35.0% | |
| TTTCTTCGCTGAATTGAATG+AGG | + | 57522:3759-3778 | MS.gene63685:CDS | 35.0% | |
| ! | AAATCTTTTCTGGACTACTG+AGG | - | 57522:4002-4021 | MS.gene63685:intergenic | 35.0% |
| ! | TTTATGTTGTAGGGTGTTCT+TGG | + | 57522:4408-4427 | MS.gene63685:intron | 35.0% |
| AAACCTTAAGACACCGTAAC+AGG | - | 57522:4146-4165 | None:intergenic | 40.0% | |
| ATGATGATTTCAGTGCGCAT+GGG | - | 57522:3841-3860 | MS.gene63685:intergenic | 40.0% | |
| ATGCCGAGAAAGTTAACAAC+AGG | + | 57522:4050-4069 | MS.gene63685:CDS | 40.0% | |
| CTTGCTGAAGATGGATATTC+CGG | + | 57522:3796-3815 | MS.gene63685:CDS | 40.0% | |
| GATTAGGTGAAAAGAGTAGG+AGG | + | 57522:3963-3982 | MS.gene63685:intron | 40.0% | |
| GTAATCCTTAACAGGTTGTC+CGG | - | 57522:4272-4291 | MS.gene63685:intergenic | 40.0% | |
| TACAGTTCAACACTGTTCTC+AGG | - | 57522:4031-4050 | MS.gene63685:intergenic | 40.0% | |
| TCCCCTGTTGTTAACTTTCT+CGG | - | 57522:4056-4075 | MS.gene63685:intergenic | 40.0% | |
| TGCCGAGAAAGTTAACAACA+GGG | + | 57522:4051-4070 | MS.gene63685:CDS | 40.0% | |
| TGTTACCATTCACACTCCAA+AGG | + | 57522:4503-4522 | MS.gene63685:CDS | 40.0% | |
| TTAGGACCTTGCTTTCCTTT+AGG | - | 57522:4465-4484 | MS.gene63685:intergenic | 40.0% | |
| ! | ATATCAGGTAGGGGAGTTTT+AGG | - | 57522:4483-4502 | MS.gene63685:intergenic | 40.0% |
| ! | ATATTCCGGTGTTGAGGTTA+GGG | + | 57522:3810-3829 | MS.gene63685:CDS | 40.0% |
| ! | GTAGGGTGTTCTTGGTATTA+AGG | + | 57522:4416-4435 | MS.gene63685:intron | 40.0% |
| ! | GTTATGGAAAGTGGTGCTAA+GGG | + | 57522:4166-4185 | MS.gene63685:CDS | 40.0% |
| ! | TGTTATGGAAAGTGGTGCTA+AGG | + | 57522:4165-4184 | MS.gene63685:CDS | 40.0% |
| !! | CGGTGTCTTAAGGTTTGTTA+TGG | + | 57522:4150-4169 | MS.gene63685:CDS | 40.0% |
| AAACAACAGGGACTAGGACA+GGG | - | 57522:4581-4600 | None:intergenic | 45.0% | |
| AAGAGACTCAGCTTGTGCAA+TGG | - | 57522:4086-4105 | None:intergenic | 45.0% | |
| AGTAACCCTAACCTCAACAC+CGG | - | 57522:3818-3837 | MS.gene63685:intergenic | 45.0% | |
| ATGCTTGACTGGGATCCTAA+AGG | + | 57522:4447-4466 | MS.gene63685:CDS | 45.0% | |
| ATGTACTCTTCCTCCTCCTT+TGG | - | 57522:4522-4541 | MS.gene63685:intergenic | 45.0% | |
| CGAACGACCAATAAGGTTGT+TGG | - | 57522:4344-4363 | MS.gene63685:intergenic | 45.0% | |
| CTTCTTTACAAGCTTCTCGG+TGG | + | 57522:4103-4122 | MS.gene63685:CDS | 45.0% | |
| GATGATGATTTCAGTGCGCA+TGG | - | 57522:3842-3861 | MS.gene63685:intergenic | 45.0% | |
| GCCGAGAAAGTTAACAACAG+GGG | + | 57522:4052-4071 | MS.gene63685:CDS | 45.0% | |
| TACCATTCACACTCCAAAGG+AGG | + | 57522:4506-4525 | MS.gene63685:CDS | 45.0% | |
| TCATTTGCAACAACAGCAGC+AGG | - | 57522:4549-4568 | MS.gene63685:intergenic | 45.0% | |
| TGGGATCCTAAAGGAAAGCA+AGG | + | 57522:4456-4475 | MS.gene63685:CDS | 45.0% | |
| ! | AGATGGATATTCCGGTGTTG+AGG | + | 57522:3804-3823 | MS.gene63685:CDS | 45.0% |
| ! | GATATTCCGGTGTTGAGGTT+AGG | + | 57522:3809-3828 | MS.gene63685:CDS | 45.0% |
| !!! | TATTTTTAATTTGATTTAAA+TGG | + | 57522:3917-3936 | MS.gene63685:intron | 5.0% |
| ACCACTAACCGAGAACAGCT+TGG | - | 57522:3879-3898 | None:intergenic | 50.0% | |
| ACTCGTACCCAAGCTGTTCT+CGG | + | 57522:3868-3887 | MS.gene63685:CDS | 50.0% | |
| CAAACAACAGGGACTAGGAC+AGG | - | 57522:4582-4601 | MS.gene63685:intergenic | 50.0% | |
| CATTCACACTCCAAAGGAGG+AGG | + | 57522:4509-4528 | MS.gene63685:CDS | 50.0% | |
| CCACTAACCGAGAACAGCTT+GGG | - | 57522:3878-3897 | MS.gene63685:intergenic | 50.0% | |
| GACGGGTATATGATCTCGTC+CGG | + | 57522:4250-4269 | MS.gene63685:CDS | 50.0% | |
| GAGCCTGTTACGGTGTCTTA+AGG | + | 57522:4140-4159 | MS.gene63685:CDS | 50.0% | |
| TCCATCTTCAGCAAGCTCTC+TGG | - | 57522:3791-3810 | MS.gene63685:intergenic | 50.0% | |
| ! | AGGAGGAGGAAGAGTACATC+AGG | + | 57522:4523-4542 | MS.gene63685:CDS | 50.0% |
| !! | ACCAGAGAGCTTGCTGAAGA+TGG | + | 57522:3787-3806 | MS.gene63685:CDS | 50.0% |
| !! | CTCCTCCTTTGGAGTGTGAA+TGG | - | 57522:4511-4530 | MS.gene63685:intergenic | 50.0% |
| CCCAAGCTGTTCTCGGTTAG+TGG | + | 57522:3875-3894 | MS.gene63685:intron | 55.0% | |
| CTCGTCCGGACAACCTGTTA+AGG | + | 57522:4264-4283 | MS.gene63685:CDS | 55.0% | |
| GCAGTTCGCAGAGCCTGTTA+CGG | + | 57522:4130-4149 | MS.gene63685:CDS | 55.0% | |
| ! | AAGTGGTGCTAAGGGCTGTG+AGG | + | 57522:4174-4193 | MS.gene63685:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57522 | gene | 3742 | 4602 | 3742 | ID=MS.gene63685 |
| 57522 | mRNA | 3742 | 4602 | 3742 | ID=MS.gene63685.t1;Parent=MS.gene63685 |
| 57522 | exon | 3742 | 3889 | 3742 | ID=MS.gene63685.t1.exon1;Parent=MS.gene63685.t1 |
| 57522 | CDS | 3742 | 3889 | 3742 | ID=cds.MS.gene63685.t1;Parent=MS.gene63685.t1 |
| 57522 | exon | 3969 | 4327 | 3969 | ID=MS.gene63685.t1.exon2;Parent=MS.gene63685.t1 |
| 57522 | CDS | 3969 | 4327 | 3969 | ID=cds.MS.gene63685.t1;Parent=MS.gene63685.t1 |
| 57522 | exon | 4420 | 4602 | 4420 | ID=MS.gene63685.t1.exon3;Parent=MS.gene63685.t1 |
| 57522 | CDS | 4420 | 4602 | 4420 | ID=cds.MS.gene63685.t1;Parent=MS.gene63685.t1 |
| Gene Sequence |
| Protein sequence |
