AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053439

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053439.t1 MTR_1g044660 100.000 239 0 0 1 239 1 239 3.63e-177 486
MS.gene053439.t1 MTR_1g023000 99.163 239 2 0 1 239 1 239 2.37e-176 484
MS.gene053439.t1 MTR_6g052140 94.538 238 8 1 1 238 1 233 6.51e-166 457
MS.gene053439.t1 MTR_6g052220 94.538 238 8 1 1 238 1 233 6.51e-166 457
MS.gene053439.t1 MTR_1g111450 86.383 235 31 1 1 234 1 235 5.09e-150 417
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053439.t1 AT5G35530 88.841 233 26 0 1 233 1 233 2.03e-153 426
MS.gene053439.t1 AT2G31610 88.987 227 25 0 1 227 1 227 4.40e-149 416
MS.gene053439.t1 AT3G53870 92.166 217 17 0 1 217 1 217 1.47e-148 414

Find 75 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTTGAACTTCATGGATT+TGG 0.206773 1.4:-29053694 None:intergenic
ATATCCATCTTTGAACTTCA+TGG 0.225934 1.4:-29053700 None:intergenic
TTAGGGCCCTGCTTTCCTTT+AGG 0.246468 1.4:-29053951 None:intergenic
GTTATTCTGGTGTTGAAGTT+AGG 0.246560 1.4:+29051845 MS.gene053439:CDS
GATGGATATATGATTTCTTC+TGG 0.289581 1.4:+29053714 MS.gene053439:CDS
CTTGCTGAAGATGGTTATTC+TGG 0.300476 1.4:+29051832 MS.gene053439:CDS
ACTTCATGGATTTGGCTCTC+TGG 0.314330 1.4:-29053686 None:intergenic
GTGTGAATGGTGACAATATC+AGG 0.336609 1.4:-29053984 None:intergenic
CTTCATGGATTTGGCTCTCT+GGG 0.348818 1.4:-29053685 None:intergenic
CTCTTCCTTTGGAGTGTGAA+TGG 0.357277 1.4:-29053997 None:intergenic
ATGTACTCCTCCTCTTCCTT+TGG 0.368386 1.4:-29054008 None:intergenic
CAAACAACGGGGACTGGGAC+AGG 0.374639 1.4:-29054068 None:intergenic
ATGATAAGCTAACCTGTCTA+AGG 0.386367 1.4:-29053782 None:intergenic
TTTGTGTTGTAGGGTGTTCT+CGG 0.388366 1.4:+29053897 MS.gene053439:intron
GGGACTTTGCGCCATTGCTC+AGG 0.406058 1.4:+29052432 MS.gene053439:CDS
TTATTCTGGTGTTGAAGTTA+GGG 0.418683 1.4:+29051846 MS.gene053439:CDS
ACGCTGAGAAGGTCAACAAC+AGG 0.438336 1.4:+29052410 MS.gene053439:CDS
CATCAGGCCTGCTGCTGTTG+TGG 0.461102 1.4:+29054028 MS.gene053439:CDS
ACAAGAGAGCTTGCTGAAGA+TGG 0.463095 1.4:+29051823 MS.gene053439:CDS
TTGTGATGTATAGGTGAAAA+GGG 0.465106 1.4:+29052316 MS.gene053439:intron
TGCGCACTGAGATCATCATC+AGG 0.467489 1.4:+29051878 MS.gene053439:CDS
AGGAAGAGGAGGAGTACATC+AGG 0.468833 1.4:+29054012 MS.gene053439:CDS
ATTTCTTTCAGGGCCTGCTA+TGG 0.473754 1.4:+29053507 MS.gene053439:intron
AGGTTAAGATCATGCTCGAC+TGG 0.485245 1.4:+29053925 MS.gene053439:CDS
TTTGTGATGTATAGGTGAAA+AGG 0.489151 1.4:+29052315 MS.gene053439:intron
ACTCGTACCCAAGCTGTTCT+CGG 0.490767 1.4:+29051904 MS.gene053439:CDS
TCGGAGGTCTTGCTGTTCGC+AGG 0.494676 1.4:+29052479 MS.gene053439:CDS
CTGGGATCCTAAAGGAAAGC+AGG 0.500197 1.4:+29053944 MS.gene053439:CDS
TAAAGCTCAACACTGTTCTC+AGG 0.503525 1.4:-29052388 None:intergenic
GACAGGGACCTCAATATCAT+TGG 0.510522 1.4:-29054051 None:intergenic
GAGACATGTGCTCCTTAGAC+AGG 0.512478 1.4:+29053770 MS.gene053439:CDS
GTAGGGTGTTCTCGGTATCA+AGG 0.512546 1.4:+29053905 MS.gene053439:intron
TCATTGGCCACAACAGCAGC+AGG 0.512718 1.4:-29054035 None:intergenic
TGATGATCTCAGTGCGCATA+GGG 0.514210 1.4:-29051873 None:intergenic
AAACCTCAACACACCATAGC+AGG 0.520448 1.4:-29053520 None:intergenic
TTCTTCTGGACAACCAGTCA+AGG 0.524528 1.4:+29053728 MS.gene053439:CDS
TATTGTCAGTGGAAAATTGA+GGG 0.525395 1.4:+29053662 MS.gene053439:CDS
TGAGTTACCGAGAACAGCTT+GGG 0.525457 1.4:-29051911 None:intergenic
CACGTGCAGGTTATTGTCAG+TGG 0.527776 1.4:+29053651 MS.gene053439:intron
AAATCCATGAAGTTCAAAGA+TGG 0.529947 1.4:+29053696 MS.gene053439:CDS
TGACCTCAGTTGTGCAGAAG+AGG 0.532735 1.4:+29052356 MS.gene053439:CDS
ATGAGTTACCGAGAACAGCT+TGG 0.534831 1.4:-29051912 None:intergenic
TTGAGGTTTGTCATGGAAAG+TGG 0.550123 1.4:+29053534 MS.gene053439:CDS
CGCTGAGAAGGTCAACAACA+GGG 0.550500 1.4:+29052411 MS.gene053439:CDS
GAGTCAATGTAGTCCTTGAC+TGG 0.552164 1.4:-29053741 None:intergenic
TTGTTGCAGTTCGTCGCTGA+CGG 0.560423 1.4:+29051769 MS.gene053439:intron
ATGATGATCTCAGTGCGCAT+AGG 0.560609 1.4:-29051874 None:intergenic
AAAACGAACCTCACATCCCT+TGG 0.567343 1.4:-29053559 None:intergenic
TGTCATGGAAAGTGGTGCCA+AGG 0.570166 1.4:+29053542 MS.gene053439:CDS
GGGCCTGCTATGGTGTGTTG+AGG 0.572929 1.4:+29053517 MS.gene053439:intron
AAACAACGGGGACTGGGACA+GGG 0.577925 1.4:-29054067 None:intergenic
AAGAGACTCAGCCTGAGCAA+TGG 0.580088 1.4:-29052443 None:intergenic
CATTCACACTCCAAAGGAAG+AGG 0.584530 1.4:+29053998 MS.gene053439:CDS
TGGTGTGTTGAGGTTTGTCA+TGG 0.586163 1.4:+29053527 MS.gene053439:CDS
TTATTGTCAGTGGAAAATTG+AGG 0.586639 1.4:+29053661 MS.gene053439:CDS
TGTTGAGCTTTACGCTGAGA+AGG 0.587026 1.4:+29052399 MS.gene053439:CDS
GGTTAAGATCATGCTCGACT+GGG 0.595146 1.4:+29053926 MS.gene053439:CDS
GATGTATAGGTGAAAAGGGA+AGG 0.595961 1.4:+29052320 MS.gene053439:intron
TCTCTTCGCTACAAGCTGCT+CGG 0.596248 1.4:+29052460 MS.gene053439:CDS
TGTTGTGGCCAATGATATTG+AGG 0.598745 1.4:+29054043 MS.gene053439:CDS
AATGGTGACAATATCAGGGA+GGG 0.600476 1.4:-29053979 None:intergenic
ATGCTCGACTGGGATCCTAA+AGG 0.600547 1.4:+29053936 MS.gene053439:CDS
TGGGATCCTAAAGGAAAGCA+GGG 0.616276 1.4:+29053945 MS.gene053439:CDS
TCACACTCCAAAGGAAGAGG+AGG 0.628998 1.4:+29054001 MS.gene053439:CDS
GTCATGGAAAGTGGTGCCAA+GGG 0.643243 1.4:+29053543 MS.gene053439:CDS
TGTGAATGGTGACAATATCA+GGG 0.645355 1.4:-29053983 None:intergenic
ATGGTGACAATATCAGGGAG+GGG 0.658335 1.4:-29053978 None:intergenic
TGTCACCATTCACACTCCAA+AGG 0.659904 1.4:+29053992 MS.gene053439:CDS
CTTCGCTACAAGCTGCTCGG+AGG 0.669048 1.4:+29052463 MS.gene053439:CDS
GCGCACTGAGATCATCATCA+GGG 0.670424 1.4:+29051879 MS.gene053439:CDS
GAACCTCTTCTGCACAACTG+AGG 0.678277 1.4:-29052359 None:intergenic
GCTGAGAAGGTCAACAACAG+GGG 0.681641 1.4:+29052412 MS.gene053439:CDS
AAGTGGTGCCAAGGGATGTG+AGG 0.685239 1.4:+29053551 MS.gene053439:CDS
GAATGGTGACAATATCAGGG+AGG 0.719800 1.4:-29053980 None:intergenic
GATGATCTCAGTGCGCATAG+GGG 0.725882 1.4:-29051872 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTATGTTTGTTTTAATT+TGG + chr1.4:29052244-29052263 MS.gene053439:intron 10.0%
!!! TATTATGTTTGTTTTAATTT+GGG + chr1.4:29052245-29052264 MS.gene053439:intron 10.0%
!! ATATATGAAATTGTTATGTA+TGG + chr1.4:29052124-29052143 MS.gene053439:intron 15.0%
!! TATTCAAGAGAATAAAAAAT+GGG - chr1.4:29053450-29053469 None:intergenic 15.0%
!!! ACTAATTTCTTAATTTGATT+TGG + chr1.4:29051740-29051759 MS.gene053439:intron 15.0%
!!! GTTTATTTTTGTTTCATTTT+TGG + chr1.4:29051980-29051999 MS.gene053439:intron 15.0%
!! AATTAGTCAACAAATAACAT+TGG - chr1.4:29053226-29053245 None:intergenic 20.0%
!! ATTATCTCATACATATTGAA+AGG - chr1.4:29052192-29052211 None:intergenic 20.0%
!! CAACAATTTCATAAATCATA+AGG - chr1.4:29052011-29052030 None:intergenic 20.0%
!! GTATTCAAGAGAATAAAAAA+TGG - chr1.4:29053451-29053470 None:intergenic 20.0%
!! TAGCTAAACATAAACAAATA+TGG - chr1.4:29052633-29052652 None:intergenic 20.0%
!! TAGTATTAATAGAGATGAAT+TGG - chr1.4:29051724-29051743 None:intergenic 20.0%
!! TATTTGTTTATGTTTAGCTA+CGG + chr1.4:29052633-29052652 MS.gene053439:intron 20.0%
!! TATTTGTTTATGTTTAGCTA+TGG + chr1.4:29052633-29052652 MS.gene053439:intron 20.0%
!!! AACATTTTAAGAACCTAAAA+TGG - chr1.4:29052726-29052745 None:intergenic 20.0%
!!! ACATTTTAAGAACCTAAAAT+GGG - chr1.4:29052725-29052744 None:intergenic 20.0%
!!! ACATTTTGTTTTTCAGAATA+AGG - chr1.4:29053107-29053126 None:intergenic 20.0%
!!! AGAAAATTACTCTAAAGAAA+CGG - chr1.4:29053058-29053077 None:intergenic 20.0%
!!! TTTTGTTCTTGTTTTGTTTT+GGG + chr1.4:29052090-29052109 MS.gene053439:intron 20.0%
!!! TTTTTGAGATTGTATGTTTT+GGG + chr1.4:29053334-29053353 MS.gene053439:intron 20.0%
!!! TTTTTGTTCTTGTTTTGTTT+TGG + chr1.4:29052089-29052108 MS.gene053439:intron 20.0%
!!! TTTTTTGAGATTGTATGTTT+TGG + chr1.4:29053333-29053352 MS.gene053439:intron 20.0%
! AATATATCGAGTACAAAAAG+AGG - chr1.4:29053375-29053394 None:intergenic 25.0%
! AATCATTATCATGCATCATA+AGG - chr1.4:29052069-29052088 None:intergenic 25.0%
! ATAGTATTAGGAGAAACAAT+TGG + chr1.4:29052575-29052594 MS.gene053439:intron 25.0%
! ATATATCGAGTACAAAAAGA+GGG - chr1.4:29053374-29053393 None:intergenic 25.0%
! GAAATTGTTATGTATGGATA+TGG + chr1.4:29052130-29052149 MS.gene053439:intron 25.0%
! GAATCTCATAGTGATATATA+TGG + chr1.4:29052541-29052560 MS.gene053439:intron 25.0%
! GTTCGAAGTTAAATAGTATT+AGG + chr1.4:29052563-29052582 MS.gene053439:intron 25.0%
! TAACATATGCTCATGTTAAA+TGG + chr1.4:29053272-29053291 MS.gene053439:intron 25.0%
! TAGGAAAAAGAAAGCAAATA+AGG - chr1.4:29053815-29053834 None:intergenic 25.0%
! TGTTAAAGCTAATAGAACTT+CGG - chr1.4:29053257-29053276 None:intergenic 25.0%
!! TATTTCAGTTTTCCTTTTAC+TGG + chr1.4:29053391-29053410 MS.gene053439:intron 25.0%
!!! AATTTTTAATTACCACCCAT+TGG - chr1.4:29053426-29053445 None:intergenic 25.0%
!!! TTTGTAGACTCTTTTTAAAG+AGG + chr1.4:29052869-29052888 MS.gene053439:intron 25.0%
!!! TTTGTGTTTTTGTGATGTAT+AGG + chr1.4:29052307-29052326 MS.gene053439:intron 25.0%
AAAGAGGACTAAATCATGTT+GGG + chr1.4:29052885-29052904 MS.gene053439:intron 30.0%
AAATCCATGAAGTTCAAAGA+TGG + chr1.4:29053696-29053715 MS.gene053439:CDS 30.0%
AATCTGCAACTATTGTAACA+TGG + chr1.4:29053302-29053321 MS.gene053439:intron 30.0%
ACATAGCAAACATAAAACAC+AGG - chr1.4:29053171-29053190 None:intergenic 30.0%
ATATCCATCTTTGAACTTCA+TGG - chr1.4:29053703-29053722 None:intergenic 30.0%
ATCTTTGAACTTCATGGATT+TGG - chr1.4:29053697-29053716 None:intergenic 30.0%
CAAATAACATTGGAACAAAG+AGG - chr1.4:29053216-29053235 None:intergenic 30.0%
CAGAAAACAGTATAACAAAC+AGG - chr1.4:29053604-29053623 None:intergenic 30.0%
GATGGATATATGATTTCTTC+TGG + chr1.4:29053714-29053733 MS.gene053439:CDS 30.0%
TAAAGAGGACTAAATCATGT+TGG + chr1.4:29052884-29052903 MS.gene053439:intron 30.0%
TATTGTCAGTGGAAAATTGA+GGG + chr1.4:29053662-29053681 MS.gene053439:CDS 30.0%
TTATTGTCAGTGGAAAATTG+AGG + chr1.4:29053661-29053680 MS.gene053439:CDS 30.0%
TTGTGATGTATAGGTGAAAA+GGG + chr1.4:29052316-29052335 MS.gene053439:intron 30.0%
TTGTTTATGTTTAGCTATGG+TGG + chr1.4:29052695-29052714 MS.gene053439:intron 30.0%
TTTGTGATGTATAGGTGAAA+AGG + chr1.4:29052315-29052334 MS.gene053439:intron 30.0%
! AAAAAGAGGGATTTTAGATC+TGG - chr1.4:29053361-29053380 None:intergenic 30.0%
! GTCTCTAACTATTTCTTTCA+GGG + chr1.4:29053497-29053516 MS.gene053439:intron 30.0%
! TGTCTCTAACTATTTCTTTC+AGG + chr1.4:29053496-29053515 MS.gene053439:intron 30.0%
! TTATTCTGGTGTTGAAGTTA+GGG + chr1.4:29051846-29051865 MS.gene053439:CDS 30.0%
! TTTCATGTTGGTTGTTCATT+TGG + chr1.4:29053840-29053859 MS.gene053439:intron 30.0%
! TTTCCTACAACTTTTCATGT+TGG + chr1.4:29053828-29053847 MS.gene053439:intron 30.0%
!! GTTGTTCATTTGGTTTGATA+TGG + chr1.4:29053850-29053869 MS.gene053439:intron 30.0%
!! TGTTTTTCAGAATAAGGAGA+TGG - chr1.4:29053101-29053120 None:intergenic 30.0%
ATGATAAGCTAACCTGTCTA+AGG - chr1.4:29053785-29053804 None:intergenic 35.0%
CAACCAACATGAAAAGTTGT+AGG - chr1.4:29053834-29053853 None:intergenic 35.0%
GTTGAGCTGTTATAACATGT+TGG + chr1.4:29053130-29053149 MS.gene053439:intron 35.0%
TACTGGTGTGTATTTCCAAT+GGG + chr1.4:29053408-29053427 MS.gene053439:intron 35.0%
TGGAAATACACACCAGTAAA+AGG - chr1.4:29053406-29053425 None:intergenic 35.0%
TGTGAATGGTGACAATATCA+GGG - chr1.4:29053986-29054005 None:intergenic 35.0%
TTACTGGTGTGTATTTCCAA+TGG + chr1.4:29053407-29053426 MS.gene053439:intron 35.0%
TTGTTTATGTTTAGCTACGG+TGG + chr1.4:29052636-29052655 MS.gene053439:intron 35.0%
! ATCATGTTGGGCATTTTTCT+TGG + chr1.4:29052897-29052916 MS.gene053439:intron 35.0%
! GTTATTCTGGTGTTGAAGTT+AGG + chr1.4:29051845-29051864 MS.gene053439:CDS 35.0%
! TGGGACTCATAAAGCATTTA+AGG - chr1.4:29052964-29052983 None:intergenic 35.0%
!! TGTAATTGTTTTTCACGTGC+AGG + chr1.4:29053638-29053657 MS.gene053439:intron 35.0%
!!! CTTTCGCTTTTTGCTCATTT+GGG - chr1.4:29051673-29051692 None:intergenic 35.0%
AAAGAAACGGTAAAGTCACC+TGG - chr1.4:29053045-29053064 None:intergenic 40.0%
AATGGTGACAATATCAGGGA+GGG - chr1.4:29053982-29054001 None:intergenic 40.0%
AGAACACCCTACAACACAAA+GGG - chr1.4:29053897-29053916 None:intergenic 40.0%
CCAAATGAGCAAAAAGCGAA+AGG + chr1.4:29051671-29051690 MS.gene053439:CDS 40.0%
CTTGCTGAAGATGGTTATTC+TGG + chr1.4:29051832-29051851 MS.gene053439:CDS 40.0%
CTTTATGAGTCCCAACTTAG+GGG + chr1.4:29052970-29052989 MS.gene053439:intron 40.0%
GATGTATAGGTGAAAAGGGA+AGG + chr1.4:29052320-29052339 MS.gene053439:intron 40.0%
GCTTTATGAGTCCCAACTTA+GGG + chr1.4:29052969-29052988 MS.gene053439:intron 40.0%
GTGTGAATGGTGACAATATC+AGG - chr1.4:29053987-29054006 None:intergenic 40.0%
TAAAGCTCAACACTGTTCTC+AGG - chr1.4:29052391-29052410 None:intergenic 40.0%
TCAAAGCTGTCTCTGATGAT+TGG + chr1.4:29052802-29052821 MS.gene053439:intron 40.0%
TGCTTTATGAGTCCCAACTT+AGG + chr1.4:29052968-29052987 MS.gene053439:intron 40.0%
TGTTGTGGCCAATGATATTG+AGG + chr1.4:29054043-29054062 MS.gene053439:CDS 40.0%
TTGAGGTTTGTCATGGAAAG+TGG + chr1.4:29053534-29053553 MS.gene053439:CDS 40.0%
TTTCAATGCACCCCTAAGTT+GGG - chr1.4:29052983-29053002 None:intergenic 40.0%
! TTTGTGTTGTAGGGTGTTCT+CGG + chr1.4:29053897-29053916 MS.gene053439:intron 40.0%
! TTTTCAATGCACCCCTAAGT+TGG - chr1.4:29052984-29053003 None:intergenic 40.0%
!! TATGGTGGAAAGCCCATTTT+AGG + chr1.4:29052710-29052729 MS.gene053439:intron 40.0%
!!! CCTTTCGCTTTTTGCTCATT+TGG - chr1.4:29051674-29051693 None:intergenic 40.0%
AAAACGAACCTCACATCCCT+TGG - chr1.4:29053562-29053581 None:intergenic 45.0%
AAACCTCAACACACCATAGC+AGG - chr1.4:29053523-29053542 None:intergenic 45.0%
AGGTTAAGATCATGCTCGAC+TGG + chr1.4:29053925-29053944 MS.gene053439:CDS 45.0%
ATGAGTTACCGAGAACAGCT+TGG - chr1.4:29051915-29051934 None:intergenic 45.0%
ATGATGATCTCAGTGCGCAT+AGG - chr1.4:29051877-29051896 None:intergenic 45.0%
ATGGTGACAATATCAGGGAG+GGG - chr1.4:29053981-29054000 None:intergenic 45.0%
ATGTACTCCTCCTCTTCCTT+TGG - chr1.4:29054011-29054030 None:intergenic 45.0%
ATTTCTTTCAGGGCCTGCTA+TGG + chr1.4:29053507-29053526 MS.gene053439:intron 45.0%
CATTCACACTCCAAAGGAAG+AGG + chr1.4:29053998-29054017 MS.gene053439:CDS 45.0%
GAATGGTGACAATATCAGGG+AGG - chr1.4:29053983-29054002 None:intergenic 45.0%
GACAGGGACCTCAATATCAT+TGG - chr1.4:29054054-29054073 None:intergenic 45.0%
GAGAACACCCTACAACACAA+AGG - chr1.4:29053898-29053917 None:intergenic 45.0%
GAGTCAATGTAGTCCTTGAC+TGG - chr1.4:29053744-29053763 None:intergenic 45.0%
GGTTAAGATCATGCTCGACT+GGG + chr1.4:29053926-29053945 MS.gene053439:CDS 45.0%
GTTGTGCCCTTTGTGTTGTA+GGG + chr1.4:29053888-29053907 MS.gene053439:intron 45.0%
TGAGTTACCGAGAACAGCTT+GGG - chr1.4:29051914-29051933 None:intergenic 45.0%
TGATGATCTCAGTGCGCATA+GGG - chr1.4:29051876-29051895 None:intergenic 45.0%
TGGGATCCTAAAGGAAAGCA+GGG + chr1.4:29053945-29053964 MS.gene053439:CDS 45.0%
TGGTGTGTATTTCCAATGGG+TGG + chr1.4:29053411-29053430 MS.gene053439:intron 45.0%
TGGTGTGTTGAGGTTTGTCA+TGG + chr1.4:29053527-29053546 MS.gene053439:CDS 45.0%
TGTCACCATTCACACTCCAA+AGG + chr1.4:29053992-29054011 MS.gene053439:CDS 45.0%
TGTTGAGCTTTACGCTGAGA+AGG + chr1.4:29052399-29052418 MS.gene053439:CDS 45.0%
TGTTGTGCCCTTTGTGTTGT+AGG + chr1.4:29053887-29053906 MS.gene053439:intron 45.0%
TTCTTCTGGACAACCAGTCA+AGG + chr1.4:29053728-29053747 MS.gene053439:CDS 45.0%
! ATATCAGGGAGGGGAGTTTT+AGG - chr1.4:29053972-29053991 None:intergenic 45.0%
! TATCAGGGAGGGGAGTTTTA+GGG - chr1.4:29053971-29053990 None:intergenic 45.0%
!! ACAAGAGAGCTTGCTGAAGA+TGG + chr1.4:29051823-29051842 MS.gene053439:CDS 45.0%
!! ACTTCATGGATTTGGCTCTC+TGG - chr1.4:29053689-29053708 None:intergenic 45.0%
!! CTCTTCCTTTGGAGTGTGAA+TGG - chr1.4:29054000-29054019 None:intergenic 45.0%
!! CTTCATGGATTTGGCTCTCT+GGG - chr1.4:29053688-29053707 None:intergenic 45.0%
AAACGGTAAAGTCACCTGGC+TGG - chr1.4:29053041-29053060 None:intergenic 50.0%
AAGAGACTCAGCCTGAGCAA+TGG - chr1.4:29052446-29052465 None:intergenic 50.0%
ACGCTGAGAAGGTCAACAAC+AGG + chr1.4:29052410-29052429 MS.gene053439:CDS 50.0%
ACTCGTACCCAAGCTGTTCT+CGG + chr1.4:29051904-29051923 MS.gene053439:CDS 50.0%
ATGCTCGACTGGGATCCTAA+AGG + chr1.4:29053936-29053955 MS.gene053439:CDS 50.0%
CACGTGCAGGTTATTGTCAG+TGG + chr1.4:29053651-29053670 MS.gene053439:intron 50.0%
CCTGAGATTGAATACCAGCC+AGG + chr1.4:29053024-29053043 MS.gene053439:intron 50.0%
CCTGGCTGGTATTCAATCTC+AGG - chr1.4:29053027-29053046 None:intergenic 50.0%
CGCTGAGAAGGTCAACAACA+GGG + chr1.4:29052411-29052430 MS.gene053439:CDS 50.0%
CTGGGATCCTAAAGGAAAGC+AGG + chr1.4:29053944-29053963 MS.gene053439:CDS 50.0%
GAACCTCTTCTGCACAACTG+AGG - chr1.4:29052362-29052381 None:intergenic 50.0%
GAGACATGTGCTCCTTAGAC+AGG + chr1.4:29053770-29053789 MS.gene053439:CDS 50.0%
GATGATCTCAGTGCGCATAG+GGG - chr1.4:29051875-29051894 None:intergenic 50.0%
GCGCACTGAGATCATCATCA+GGG + chr1.4:29051879-29051898 MS.gene053439:CDS 50.0%
GCTGAGAAGGTCAACAACAG+GGG + chr1.4:29052412-29052431 MS.gene053439:CDS 50.0%
TCACACTCCAAAGGAAGAGG+AGG + chr1.4:29054001-29054020 MS.gene053439:CDS 50.0%
TCTCTTCGCTACAAGCTGCT+CGG + chr1.4:29052460-29052479 MS.gene053439:CDS 50.0%
TGACCTCAGTTGTGCAGAAG+AGG + chr1.4:29052356-29052375 MS.gene053439:CDS 50.0%
TGCGCACTGAGATCATCATC+AGG + chr1.4:29051878-29051897 MS.gene053439:CDS 50.0%
TTAGGGCCCTGCTTTCCTTT+AGG - chr1.4:29053954-29053973 None:intergenic 50.0%
TTGTTGCAGTTCGTCGCTGA+CGG + chr1.4:29051769-29051788 MS.gene053439:intron 50.0%
! AGGAAGAGGAGGAGTACATC+AGG + chr1.4:29054012-29054031 MS.gene053439:CDS 50.0%
! GTAGGGTGTTCTCGGTATCA+AGG + chr1.4:29053905-29053924 MS.gene053439:intron 50.0%
! GTCATGGAAAGTGGTGCCAA+GGG + chr1.4:29053543-29053562 MS.gene053439:CDS 50.0%
! TGTCATGGAAAGTGGTGCCA+AGG + chr1.4:29053542-29053561 MS.gene053439:CDS 50.0%
AAACAACGGGGACTGGGACA+GGG - chr1.4:29054070-29054089 None:intergenic 55.0%
TCATTGGCCACAACAGCAGC+AGG - chr1.4:29054038-29054057 None:intergenic 55.0%
! AAGTGGTGCCAAGGGATGTG+AGG + chr1.4:29053551-29053570 MS.gene053439:CDS 55.0%
CAAACAACGGGGACTGGGAC+AGG - chr1.4:29054071-29054090 None:intergenic 60.0%
CATCAGGCCTGCTGCTGTTG+TGG + chr1.4:29054028-29054047 MS.gene053439:CDS 60.0%
CTTCGCTACAAGCTGCTCGG+AGG + chr1.4:29052463-29052482 MS.gene053439:CDS 60.0%
GGGACTTTGCGCCATTGCTC+AGG + chr1.4:29052432-29052451 MS.gene053439:CDS 60.0%
GGGCCTGCTATGGTGTGTTG+AGG + chr1.4:29053517-29053536 MS.gene053439:intron 60.0%
!! TCGGAGGTCTTGCTGTTCGC+AGG + chr1.4:29052479-29052498 MS.gene053439:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 29051663 29054091 29051663 ID=MS.gene053439
chr1.4 mRNA 29051663 29054091 29051663 ID=MS.gene053439.t1;Parent=MS.gene053439
chr1.4 exon 29051663 29051692 29051663 ID=MS.gene053439.t1.exon1;Parent=MS.gene053439.t1
chr1.4 CDS 29051663 29051692 29051663 ID=cds.MS.gene053439.t1;Parent=MS.gene053439.t1
chr1.4 exon 29051778 29051925 29051778 ID=MS.gene053439.t1.exon2;Parent=MS.gene053439.t1
chr1.4 CDS 29051778 29051925 29051778 ID=cds.MS.gene053439.t1;Parent=MS.gene053439.t1
chr1.4 exon 29052329 29052500 29052329 ID=MS.gene053439.t1.exon3;Parent=MS.gene053439.t1
chr1.4 CDS 29052329 29052500 29052329 ID=cds.MS.gene053439.t1;Parent=MS.gene053439.t1
chr1.4 exon 29053518 29053572 29053518 ID=MS.gene053439.t1.exon4;Parent=MS.gene053439.t1
chr1.4 CDS 29053518 29053572 29053518 ID=cds.MS.gene053439.t1;Parent=MS.gene053439.t1
chr1.4 exon 29053660 29053791 29053660 ID=MS.gene053439.t1.exon5;Parent=MS.gene053439.t1
chr1.4 CDS 29053660 29053791 29053660 ID=cds.MS.gene053439.t1;Parent=MS.gene053439.t1
chr1.4 exon 29053909 29054091 29053909 ID=MS.gene053439.t1.exon6;Parent=MS.gene053439.t1
chr1.4 CDS 29053909 29054091 29053909 ID=cds.MS.gene053439.t1;Parent=MS.gene053439.t1
Gene Sequence

>MS.gene053439

ATGGCTACCCAAATGAGCAAAAAGCGAAAGGTTAGTTCTCAGATTCGTCAATCTTAATCCAATTCATCTCTATTAATACTAATTTCTTAATTTGATTTGGTTTGTGTTGTTGCAGTTCGTCGCTGACGGAGTTTTCTTCGCTGAATTGAATGAAGTTTTGACAAGAGAGCTTGCTGAAGATGGTTATTCTGGTGTTGAAGTTAGGGTTACCCCTATGCGCACTGAGATCATCATCAGGGCTACTCGTACCCAAGCTGTTCTCGGTAACTCATTTCATCAATCTTGTTGTTTCAATCTCATTTTATGATTATGATTTTGTTTATTTTTGTTTCATTTTTGGTTTTTCCTTATGATTTATGAAATTGTTGTATATCGATACATGATTTCAATTTCATTTGTTTTTCCTTATGATGCATGATAATGATTTTTTTGTTCTTGTTTTGTTTTGGGTTTTGTATATGATATATGAAATTGTTATGTATGGATATGGTTATGAATATGTGATTTTAGTTTTATTTGTATTTTTCCTTTCAATATGTATGAGATAATGATTTTCTTACTTACTTTTTTTTTCTAGTGATATATTATGTTTGTTTTAATTTGGGTTTTAATCATGTGATAATGATTTATGATTTGTGTTTTATTTTGTGTTTTTGTGATGTATAGGTGAAAAGGGAAGGAGAATTAGAGAGCTGACCTCAGTTGTGCAGAAGAGGTTCAAGTTTCCTGAGAACAGTGTTGAGCTTTACGCTGAGAAGGTCAACAACAGGGGACTTTGCGCCATTGCTCAGGCTGAGTCTCTTCGCTACAAGCTGCTCGGAGGTCTTGCTGTTCGCAGGTATATATACATAACATGTTGCAATTGCTTTTGATGAAATGAATCTCATAGTGATATATATGGTTCGAAGTTAAATAGTATTAGGAGAAACAATTGGAACTGATTATTTTCTGTAATTATGAATTTGAACCATATTTGTTTATGTTTAGCTACGGTGGATGATTATTTTCTGTAATTATGAATTTGTACCATATTTGTTTATGTTTAGCTATGGTGGAAAGCCCATTTTAGGTTCTTAAAATGTTCAGCATTTTTTTCTTTGTAAAAAAGATCATGAATATATATATATATATTTCAACACTCAAAGCTGTCTCTGATGATTGGATTATTGTTTCTTGATTTTGTAATTTTAATAGAGTATTGAAGTATTTGTAGACTCTTTTTAAAGAGGACTAAATCATGTTGGGCATTTTTCTTGGCGACTAGAATGAATATTTAATCTTGATTTAAACTAGTCTAGCCTTAAATGCTTTATGAGTCCCAACTTAGGGGTGCATTGAAAAATTCTCTGTGACTACTTTGTCCTGAGATTGAATACCAGCCAGGTGACTTTACCGTTTCTTTAGAGTAATTTTCTCTTGCTCGATACATTACTCACCATCTCCTTATTCTGAAAAACAAAATGTGATGTTGAGCTGTTATAACATGTTGGCTCGTTACTATCTAACCTGTGTTTTATGTTTGCTATGTGCTATAAAACTGAAATCATTTTCCTCTTTGTTCCAATGTTATTTGTTGACTAATTGTATTACACCGAAGTTCTATTAGCTTTAACATATGCTCATGTTAAATGGTACACTTAATCTGCAACTATTGTAACATGGTGATTTACTTTTTTGAGATTGTATGTTTTGGGTCCAGATCTAAAATCCCTCTTTTTGTACTCGATATATTTCAGTTTTCCTTTTACTGGTGTGTATTTCCAATGGGTGGTAATTAAAAATTTCCCATTTTTTATTCTCTTGAATACAGTTTTGTGCTAACATAATGAACTGTGTCTCTAACTATTTCTTTCAGGGCCTGCTATGGTGTGTTGAGGTTTGTCATGGAAAGTGGTGCCAAGGGATGTGAGGTTCGTTTTACATAAACTACATGAAACTCCTGTTTGTTATACTGTTTTCTGTAATGAATTTTAGTTGTAATTGTTTTTCACGTGCAGGTTATTGTCAGTGGAAAATTGAGGGCCCAGAGAGCCAAATCCATGAAGTTCAAAGATGGATATATGATTTCTTCTGGACAACCAGTCAAGGACTACATTGACTCTGCTGTGAGACATGTGCTCCTTAGACAGGTTAGCTTATCATCTAAGAACCTTATTTGCTTTCTTTTTCCTACAACTTTTCATGTTGGTTGTTCATTTGGTTTGATATGGATTTACGTTGCTAATGTTGTGCCCTTTGTGTTGTAGGGTGTTCTCGGTATCAAGGTTAAGATCATGCTCGACTGGGATCCTAAAGGAAAGCAGGGCCCTAAAACTCCCCTCCCTGATATTGTCACCATTCACACTCCAAAGGAAGAGGAGGAGTACATCAGGCCTGCTGCTGTTGTGGCCAATGATATTGAGGTCCCTGTCCCAGTCCCCGTTGTTTGA
Protein sequence

>MS.gene053439.t1

MATQMSKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQAVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMISSGQPVKDYIDSAVRHVLLRQGVLGIKVKIMLDWDPKGKQGPKTPLPDIVTIHTPKEEEEYIRPAAVVANDIEVPVPVPVV