AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24232


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24232.t1 MTR_1g044660 100.000 239 0 0 1 239 1 239 3.63e-177 486
MS.gene24232.t1 MTR_1g023000 99.163 239 2 0 1 239 1 239 2.37e-176 484
MS.gene24232.t1 MTR_6g052140 94.538 238 8 1 1 238 1 233 6.51e-166 457
MS.gene24232.t1 MTR_6g052220 94.538 238 8 1 1 238 1 233 6.51e-166 457
MS.gene24232.t1 MTR_1g111450 86.383 235 31 1 1 234 1 235 5.09e-150 417
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24232.t1 AT5G35530 88.841 233 26 0 1 233 1 233 2.03e-153 426
MS.gene24232.t1 AT2G31610 88.987 227 25 0 1 227 1 227 4.40e-149 416
MS.gene24232.t1 AT3G53870 92.166 217 17 0 1 217 1 217 1.47e-148 414

Find 67 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGGGTGTTCTTGGTATTA+AGG 0.259759 1.2:+1855827 MS.gene24232:intron
TTAGGACCTTGCTTTCCTTT+AGG 0.279269 1.2:-1855873 None:intergenic
GCTATTCCGGTGTTGAGGTT+AGG 0.315077 1.2:+1855214 MS.gene24232:CDS
CTTGCTGAAGATGGCTATTC+CGG 0.326131 1.2:+1855201 MS.gene24232:CDS
TCCCCTGTTGTTAACCTTCT+CGG 0.326817 1.2:-1855464 None:intergenic
CGGTGTGTTGAGGTTTGTTA+TGG 0.332791 1.2:+1855561 MS.gene24232:CDS
ACTCGTACCCAAGCTGTTCT+TGG 0.341791 1.2:+1855273 MS.gene24232:CDS
GATGTACTCTTCCTCCTCCT+TGG 0.351127 1.2:-1855931 None:intergenic
AAACCTCAACACACCGTAAC+AGG 0.355716 1.2:-1855554 None:intergenic
CTCATCTGGCCAACCTGTTA+AGG 0.357556 1.2:+1855675 MS.gene24232:CDS
ATAGCCATCCTTGAACTTCA+TGG 0.360301 1.2:-1855647 None:intergenic
CTATTCCGGTGTTGAGGTTA+GGG 0.371348 1.2:+1855215 MS.gene24232:CDS
GGATGTGAGGTTATTGTTAG+TGG 0.386156 1.2:+1855598 MS.gene24232:CDS
GAGTCAATGTAATCCTTAAC+AGG 0.402767 1.2:-1855688 None:intergenic
ATCCTTGAACTTCATGGACT+TGG 0.424680 1.2:-1855641 None:intergenic
ATGTACTCTTCCTCCTCCTT+GGG 0.427577 1.2:-1855930 None:intergenic
GTGTGAATGGTAACAATATC+AGG 0.428011 1.2:-1855906 None:intergenic
AGGTGAAGATTATGCTTGAC+TGG 0.432560 1.2:+1855847 MS.gene24232:CDS
CTCCTCCTTGGGAGTGTGAA+TGG 0.434959 1.2:-1855919 None:intergenic
ACCAGAGAGCTTGCTGAAGA+TGG 0.438488 1.2:+1855192 MS.gene24232:CDS
GATGGCTATATGATCTCATC+TGG 0.452022 1.2:+1855661 MS.gene24232:CDS
TCTCTTCGTTACAAGCTTCT+CGG 0.453319 1.2:+1855511 MS.gene24232:CDS
ATGCCGAGAAGGTTAACAAC+AGG 0.456910 1.2:+1855461 MS.gene24232:CDS
CAAACAACAGGGACTGGGAC+AGG 0.457728 1.2:-1855990 None:intergenic
AGGAGGAGGAAGAGTACATC+AGG 0.462947 1.2:+1855934 MS.gene24232:CDS
TTGAGGTTTGTTATGGAAAG+TGG 0.475116 1.2:+1855568 MS.gene24232:CDS
AATGATTAGGTGAGAAGGGA+AGG 0.478164 1.2:+1855371 MS.gene24232:intron
GATTAGGTGAGAAGGGAAGG+AGG 0.484267 1.2:+1855374 MS.gene24232:intron
TATAGTTCAACACTGTTCTC+AGG 0.488325 1.2:-1855439 None:intergenic
GCCATCTTCAGCAAGCTCTC+TGG 0.491889 1.2:-1855193 None:intergenic
AGCCAAGTCCATGAAGTTCA+AGG 0.492737 1.2:+1855639 MS.gene24232:CDS
TGTTATGGAAAGTGGTGCTA+AGG 0.499768 1.2:+1855576 MS.gene24232:CDS
TAGACATGTTCTTCTCAGAC+AGG 0.500852 1.2:+1855717 MS.gene24232:CDS
TTGTTGCAGTTCGTCGCTGA+CGG 0.501712 1.2:+1855138 MS.gene24232:intron
TGACCTCAGTAGTCCAGAAA+AGG 0.517945 1.2:+1855407 MS.gene24232:CDS
CTACGAACCAAGAACAGCTT+GGG 0.525454 1.2:-1855280 None:intergenic
GATGATGATCTCAGTGCGCA+TGG 0.527229 1.2:-1855244 None:intergenic
TCATTTGCAACAACAGCAGC+AGG 0.528096 1.2:-1855957 None:intergenic
GATTAATGATTAGGTGAGAA+GGG 0.529224 1.2:+1855367 MS.gene24232:intron
TTTCTTCGCTGAATTGAATG+AGG 0.530015 1.2:+1855164 MS.gene24232:CDS
AATGGTAACAATATCAGGTA+GGG 0.532486 1.2:-1855901 None:intergenic
GCTGTTCGCAGAGCCTGTTA+CGG 0.553152 1.2:+1855541 MS.gene24232:CDS
GAGCCTGTTACGGTGTGTTG+AGG 0.556363 1.2:+1855551 MS.gene24232:CDS
TGATTAATGATTAGGTGAGA+AGG 0.556668 1.2:+1855366 MS.gene24232:intron
ATGCTTGACTGGGATCCTAA+AGG 0.556783 1.2:+1855858 MS.gene24232:CDS
TGTTGTTGCAAATGATATTG+AGG 0.562213 1.2:+1855965 MS.gene24232:CDS
ACTACGAACCAAGAACAGCT+TGG 0.565206 1.2:-1855281 None:intergenic
TTTCTCAAACAACAGGGACT+GGG 0.568525 1.2:-1855995 None:intergenic
GTTATGGAAAGTGGTGCTAA+GGG 0.582081 1.2:+1855577 MS.gene24232:CDS
GAATGGTAACAATATCAGGT+AGG 0.585408 1.2:-1855902 None:intergenic
AGATGGCTATTCCGGTGTTG+AGG 0.587442 1.2:+1855209 MS.gene24232:CDS
CATTCACACTCCCAAGGAGG+AGG 0.589806 1.2:+1855920 MS.gene24232:CDS
CAATGTAATCCTTAACAGGT+TGG 0.596885 1.2:-1855684 None:intergenic
TGGGATCCTAAAGGAAAGCA+AGG 0.601952 1.2:+1855867 MS.gene24232:CDS
GGTGAAGATTATGCTTGACT+GGG 0.603451 1.2:+1855848 MS.gene24232:CDS
AAACAACAGGGACTGGGACA+GGG 0.607283 1.2:-1855989 None:intergenic
TGTTGAACTATATGCCGAGA+AGG 0.615665 1.2:+1855450 MS.gene24232:CDS
ATGGTAACAATATCAGGTAG+GGG 0.623679 1.2:-1855900 None:intergenic
TGTTACCATTCACACTCCCA+AGG 0.629341 1.2:+1855914 MS.gene24232:CDS
AAGTCCATGAAGTTCAAGGA+TGG 0.640546 1.2:+1855643 MS.gene24232:CDS
ATGATGATCTCAGTGCGCAT+GGG 0.667262 1.2:-1855243 None:intergenic
AAGTGGTGCTAAGGGATGTG+AGG 0.670962 1.2:+1855585 MS.gene24232:CDS
CTTCGTTACAAGCTTCTCGG+AGG 0.671532 1.2:+1855514 MS.gene24232:CDS
TGCCGAGAAGGTTAACAACA+GGG 0.674234 1.2:+1855462 MS.gene24232:CDS
AGTAACCCTAACCTCAACAC+CGG 0.681090 1.2:-1855220 None:intergenic
GCCGAGAAGGTTAACAACAG+GGG 0.682645 1.2:+1855463 MS.gene24232:CDS
TACCATTCACACTCCCAAGG+AGG 0.690748 1.2:+1855917 MS.gene24232:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTATATGAATTTAAA+TGG + chr1.2:1855336-1855355 MS.gene24232:intron 10.0%
!!! ATTATTACCTTTATTTTGTA+GGG + chr1.2:1855810-1855829 MS.gene24232:intron 15.0%
!!! GATTATTACCTTTATTTTGT+AGG + chr1.2:1855809-1855828 MS.gene24232:intron 20.0%
! GTTGAATCTGATTAATGATT+AGG + chr1.2:1855358-1855377 MS.gene24232:intron 25.0%
AAACCAACAAGGTTGTTATA+GGG - chr1.2:1855755-1855774 None:intergenic 30.0%
AAGAACACCCTACAAAATAA+AGG - chr1.2:1855820-1855839 None:intergenic 30.0%
AATAACAAACCAAACCAACA+AGG - chr1.2:1855766-1855785 None:intergenic 30.0%
AATGGTAACAATATCAGGTA+GGG - chr1.2:1855904-1855923 None:intergenic 30.0%
CACTACTAATAGAGATGAAT+TGG - chr1.2:1855077-1855096 None:intergenic 30.0%
GATTAATGATTAGGTGAGAA+GGG + chr1.2:1855367-1855386 MS.gene24232:intron 30.0%
TGATTAATGATTAGGTGAGA+AGG + chr1.2:1855366-1855385 MS.gene24232:intron 30.0%
TGTTGTTGCAAATGATATTG+AGG + chr1.2:1855965-1855984 MS.gene24232:CDS 30.0%
!!! TTTATTTTGTAGGGTGTTCT+TGG + chr1.2:1855819-1855838 MS.gene24232:intron 30.0%
ATGGTAACAATATCAGGTAG+GGG - chr1.2:1855903-1855922 None:intergenic 35.0%
CAAACCAACAAGGTTGTTAT+AGG - chr1.2:1855756-1855775 None:intergenic 35.0%
CAATGTAATCCTTAACAGGT+TGG - chr1.2:1855687-1855706 None:intergenic 35.0%
GAATGGTAACAATATCAGGT+AGG - chr1.2:1855905-1855924 None:intergenic 35.0%
GAGTCAATGTAATCCTTAAC+AGG - chr1.2:1855691-1855710 None:intergenic 35.0%
GTGTGAATGGTAACAATATC+AGG - chr1.2:1855909-1855928 None:intergenic 35.0%
TATAGTTCAACACTGTTCTC+AGG - chr1.2:1855442-1855461 None:intergenic 35.0%
TTACCCTATAACAACCTTGT+TGG + chr1.2:1855749-1855768 MS.gene24232:intron 35.0%
TTTCTTCGCTGAATTGAATG+AGG + chr1.2:1855164-1855183 MS.gene24232:CDS 35.0%
! CAGGAAACTTAAACCTTTTC+TGG - chr1.2:1855423-1855442 None:intergenic 35.0%
! CTATAACAACCTTGTTGGTT+TGG + chr1.2:1855754-1855773 MS.gene24232:intron 35.0%
!! TTGAGGTTTGTTATGGAAAG+TGG + chr1.2:1855568-1855587 MS.gene24232:CDS 35.0%
!!! CTTTCGCTTTTTGCTCATTT+GGG - chr1.2:1855026-1855045 None:intergenic 35.0%
AAGTCCATGAAGTTCAAGGA+TGG + chr1.2:1855643-1855662 MS.gene24232:CDS 40.0%
AATGATTAGGTGAGAAGGGA+AGG + chr1.2:1855371-1855390 MS.gene24232:intron 40.0%
AGGTGAAGATTATGCTTGAC+TGG + chr1.2:1855847-1855866 MS.gene24232:CDS 40.0%
ATAGCCATCCTTGAACTTCA+TGG - chr1.2:1855650-1855669 None:intergenic 40.0%
ATCCTTGAACTTCATGGACT+TGG - chr1.2:1855644-1855663 None:intergenic 40.0%
CCAAATGAGCAAAAAGCGAA+AGG + chr1.2:1855024-1855043 MS.gene24232:CDS 40.0%
GATGGCTATATGATCTCATC+TGG + chr1.2:1855661-1855680 MS.gene24232:CDS 40.0%
GGATGTGAGGTTATTGTTAG+TGG + chr1.2:1855598-1855617 MS.gene24232:CDS 40.0%
GGTGAAGATTATGCTTGACT+GGG + chr1.2:1855848-1855867 MS.gene24232:CDS 40.0%
TAGACATGTTCTTCTCAGAC+AGG + chr1.2:1855717-1855736 MS.gene24232:CDS 40.0%
TCTCTTCGTTACAAGCTTCT+CGG + chr1.2:1855511-1855530 MS.gene24232:CDS 40.0%
TGTTGAACTATATGCCGAGA+AGG + chr1.2:1855450-1855469 MS.gene24232:CDS 40.0%
TTAGGACCTTGCTTTCCTTT+AGG - chr1.2:1855876-1855895 None:intergenic 40.0%
! AAACCTTTTCTGGACTACTG+AGG - chr1.2:1855413-1855432 None:intergenic 40.0%
! ATATCAGGTAGGGGAGTTTT+AGG - chr1.2:1855894-1855913 None:intergenic 40.0%
! GTAGGGTGTTCTTGGTATTA+AGG + chr1.2:1855827-1855846 MS.gene24232:intron 40.0%
! GTTATGGAAAGTGGTGCTAA+GGG + chr1.2:1855577-1855596 MS.gene24232:CDS 40.0%
! TGTTATGGAAAGTGGTGCTA+AGG + chr1.2:1855576-1855595 MS.gene24232:CDS 40.0%
!!! CCTTTCGCTTTTTGCTCATT+TGG - chr1.2:1855027-1855046 None:intergenic 40.0%
AAACCTCAACACACCGTAAC+AGG - chr1.2:1855557-1855576 None:intergenic 45.0%
ACTACGAACCAAGAACAGCT+TGG - chr1.2:1855284-1855303 None:intergenic 45.0%
AGCCAAGTCCATGAAGTTCA+AGG + chr1.2:1855639-1855658 MS.gene24232:CDS 45.0%
AGTAACCCTAACCTCAACAC+CGG - chr1.2:1855223-1855242 None:intergenic 45.0%
ATGATGATCTCAGTGCGCAT+GGG - chr1.2:1855246-1855265 None:intergenic 45.0%
ATGCCGAGAAGGTTAACAAC+AGG + chr1.2:1855461-1855480 MS.gene24232:CDS 45.0%
ATGCTTGACTGGGATCCTAA+AGG + chr1.2:1855858-1855877 MS.gene24232:CDS 45.0%
ATGTACTCTTCCTCCTCCTT+GGG - chr1.2:1855933-1855952 None:intergenic 45.0%
CTACGAACCAAGAACAGCTT+GGG - chr1.2:1855283-1855302 None:intergenic 45.0%
CTTGCTGAAGATGGCTATTC+CGG + chr1.2:1855201-1855220 MS.gene24232:CDS 45.0%
TCATTTGCAACAACAGCAGC+AGG - chr1.2:1855960-1855979 None:intergenic 45.0%
TCCCCTGTTGTTAACCTTCT+CGG - chr1.2:1855467-1855486 None:intergenic 45.0%
TGACCTCAGTAGTCCAGAAA+AGG + chr1.2:1855407-1855426 MS.gene24232:CDS 45.0%
TGCCGAGAAGGTTAACAACA+GGG + chr1.2:1855462-1855481 MS.gene24232:CDS 45.0%
TGGGATCCTAAAGGAAAGCA+AGG + chr1.2:1855867-1855886 MS.gene24232:CDS 45.0%
TGTTACCATTCACACTCCCA+AGG + chr1.2:1855914-1855933 MS.gene24232:CDS 45.0%
! CTATTCCGGTGTTGAGGTTA+GGG + chr1.2:1855215-1855234 MS.gene24232:CDS 45.0%
!! CGGTGTGTTGAGGTTTGTTA+TGG + chr1.2:1855561-1855580 MS.gene24232:CDS 45.0%
AAACAACAGGGACTGGGACA+GGG - chr1.2:1855992-1856011 None:intergenic 50.0%
ACTCGTACCCAAGCTGTTCT+TGG + chr1.2:1855273-1855292 MS.gene24232:CDS 50.0%
CTCATCTGGCCAACCTGTTA+AGG + chr1.2:1855675-1855694 MS.gene24232:CDS 50.0%
CTTCGTTACAAGCTTCTCGG+AGG + chr1.2:1855514-1855533 MS.gene24232:CDS 50.0%
GATGATGATCTCAGTGCGCA+TGG - chr1.2:1855247-1855266 None:intergenic 50.0%
GATGTACTCTTCCTCCTCCT+TGG - chr1.2:1855934-1855953 None:intergenic 50.0%
GATTAGGTGAGAAGGGAAGG+AGG + chr1.2:1855374-1855393 MS.gene24232:intron 50.0%
GCCGAGAAGGTTAACAACAG+GGG + chr1.2:1855463-1855482 MS.gene24232:CDS 50.0%
TACCATTCACACTCCCAAGG+AGG + chr1.2:1855917-1855936 MS.gene24232:CDS 50.0%
TTGTTGCAGTTCGTCGCTGA+CGG + chr1.2:1855138-1855157 MS.gene24232:intron 50.0%
! AAGTGGTGCTAAGGGATGTG+AGG + chr1.2:1855585-1855604 MS.gene24232:CDS 50.0%
! AGATGGCTATTCCGGTGTTG+AGG + chr1.2:1855209-1855228 MS.gene24232:CDS 50.0%
! AGGAGGAGGAAGAGTACATC+AGG + chr1.2:1855934-1855953 MS.gene24232:CDS 50.0%
! GCTATTCCGGTGTTGAGGTT+AGG + chr1.2:1855214-1855233 MS.gene24232:CDS 50.0%
!! ACCAGAGAGCTTGCTGAAGA+TGG + chr1.2:1855192-1855211 MS.gene24232:CDS 50.0%
CAAACAACAGGGACTGGGAC+AGG - chr1.2:1855993-1856012 None:intergenic 55.0%
CATTCACACTCCCAAGGAGG+AGG + chr1.2:1855920-1855939 MS.gene24232:CDS 55.0%
GAGCCTGTTACGGTGTGTTG+AGG + chr1.2:1855551-1855570 MS.gene24232:CDS 55.0%
GCCATCTTCAGCAAGCTCTC+TGG - chr1.2:1855196-1855215 None:intergenic 55.0%
GCTGTTCGCAGAGCCTGTTA+CGG + chr1.2:1855541-1855560 MS.gene24232:CDS 55.0%
! CTCCTCCTTGGGAGTGTGAA+TGG - chr1.2:1855922-1855941 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 1855016 1856013 1855016 ID=MS.gene24232
chr1.2 mRNA 1855016 1856013 1855016 ID=MS.gene24232.t1;Parent=MS.gene24232
chr1.2 exon 1855016 1855045 1855016 ID=MS.gene24232.t1.exon1;Parent=MS.gene24232.t1
chr1.2 CDS 1855016 1855045 1855016 ID=cds.MS.gene24232.t1;Parent=MS.gene24232.t1
chr1.2 exon 1855147 1855294 1855147 ID=MS.gene24232.t1.exon2;Parent=MS.gene24232.t1
chr1.2 CDS 1855147 1855294 1855147 ID=cds.MS.gene24232.t1;Parent=MS.gene24232.t1
chr1.2 exon 1855380 1855738 1855380 ID=MS.gene24232.t1.exon3;Parent=MS.gene24232.t1
chr1.2 CDS 1855380 1855738 1855380 ID=cds.MS.gene24232.t1;Parent=MS.gene24232.t1
chr1.2 exon 1855831 1856013 1855831 ID=MS.gene24232.t1.exon4;Parent=MS.gene24232.t1
chr1.2 CDS 1855831 1856013 1855831 ID=cds.MS.gene24232.t1;Parent=MS.gene24232.t1
Gene Sequence

>MS.gene24232

ATGGCTACCCAAATGAGCAAAAAGCGAAAGGTTTGTTTTAAGATTCGTTAATCTTAATCCAATTCATCTCTATTAGTAGTGATTTCTTAATAATTCTTAATTTGACAATTTGATTTGTGTTGTTGTTGCAGTTCGTCGCTGACGGAGTTTTCTTCGCTGAATTGAATGAGGTTTTAACCAGAGAGCTTGCTGAAGATGGCTATTCCGGTGTTGAGGTTAGGGTTACTCCCATGCGCACTGAGATCATCATCAGAGCTACTCGTACCCAAGCTGTTCTTGGTTCGTAGTCATTAATTTACAAATCTGATTTTATTTATTTTTGTTTTTATATGAATTTAAATGGTTGAATCTGATTAATGATTAGGTGAGAAGGGAAGGAGGATCAGAGAACTGACCTCAGTAGTCCAGAAAAGGTTTAAGTTTCCTGAGAACAGTGTTGAACTATATGCCGAGAAGGTTAACAACAGGGGACTTTGTGCAATTGCACAAGCTGAGTCTCTTCGTTACAAGCTTCTCGGAGGTCTTGCTGTTCGCAGAGCCTGTTACGGTGTGTTGAGGTTTGTTATGGAAAGTGGTGCTAAGGGATGTGAGGTTATTGTTAGTGGAAAATTGAGAGCACAAAGAGCCAAGTCCATGAAGTTCAAGGATGGCTATATGATCTCATCTGGCCAACCTGTTAAGGATTACATTGACTCTGCTGTTAGACATGTTCTTCTCAGACAGGTTAGTTAGTTTACCCTATAACAACCTTGTTGGTTTGGTTTGTTATTGATTTATTTATGTTGCTAATGATGATTATTACCTTTATTTTGTAGGGTGTTCTTGGTATTAAGGTGAAGATTATGCTTGACTGGGATCCTAAAGGAAAGCAAGGTCCTAAAACTCCCCTACCTGATATTGTTACCATTCACACTCCCAAGGAGGAGGAAGAGTACATCAGGCCTGCTGCTGTTGTTGCAAATGATATTGAGGTCCCTGTCCCAGTCCCTGTTGTTTGA

Protein sequence

>MS.gene24232.t1

MATQMSKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQAVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMISSGQPVKDYIDSAVRHVLLRQGVLGIKVKIMLDWDPKGKQGPKTPLPDIVTIHTPKEEEEYIRPAAVVANDIEVPVPVPVV