AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27265


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27265.t1 MTR_1g044660 100.000 239 0 0 1 239 1 239 3.63e-177 486
MS.gene27265.t1 MTR_1g023000 99.163 239 2 0 1 239 1 239 2.37e-176 484
MS.gene27265.t1 MTR_6g052140 94.538 238 8 1 1 238 1 233 6.51e-166 457
MS.gene27265.t1 MTR_6g052220 94.538 238 8 1 1 238 1 233 6.51e-166 457
MS.gene27265.t1 MTR_1g111450 86.383 235 31 1 1 234 1 235 5.09e-150 417
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27265.t1 AT5G35530 88.841 233 26 0 1 233 1 233 2.03e-153 426
MS.gene27265.t1 AT2G31610 88.987 227 25 0 1 227 1 227 4.40e-149 416
MS.gene27265.t1 AT3G53870 92.166 217 17 0 1 217 1 217 1.47e-148 414

Find 66 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGGGTGTTCTTGGTATTA+AGG 0.259759 1.1:+1572278 MS.gene27265:intron
TTAGGACCTTGCTTTCCTTT+AGG 0.279269 1.1:-1572324 None:intergenic
GCTATTCCGGTGTTGAGGTT+AGG 0.315077 1.1:+1571653 MS.gene27265:CDS
CTTGCTGAAGATGGCTATTC+CGG 0.326131 1.1:+1571640 MS.gene27265:CDS
TCCCCTGTTGTTAACCTTCT+CGG 0.326817 1.1:-1571915 None:intergenic
CGGTGTGTTGAGGTTTGTTA+TGG 0.332791 1.1:+1572012 MS.gene27265:CDS
ACTCGTACCCAAGCTGTTCT+TGG 0.341791 1.1:+1571712 MS.gene27265:CDS
AAACCTCAACACACCGTAAC+AGG 0.355716 1.1:-1572005 None:intergenic
CTCATCTGGCCAACCTGTTA+AGG 0.357556 1.1:+1572126 MS.gene27265:CDS
ATAGCCATCCTTGAACTTCA+TGG 0.360301 1.1:-1572098 None:intergenic
ATGTACTCTTCCTCCTCCTT+TGG 0.368666 1.1:-1572381 None:intergenic
CTATTCCGGTGTTGAGGTTA+GGG 0.371348 1.1:+1571654 MS.gene27265:CDS
GGATGTGAGGTTATTGTTAG+TGG 0.386156 1.1:+1572049 MS.gene27265:CDS
GAGTCAATGTAATCCTTAAC+AGG 0.402767 1.1:-1572139 None:intergenic
CTCCTCCTTTGGAGTGTGAA+TGG 0.408543 1.1:-1572370 None:intergenic
ATCCTTGAACTTCATGGACT+TGG 0.424680 1.1:-1572092 None:intergenic
GTGTGAATGGTAACAATATC+AGG 0.428011 1.1:-1572357 None:intergenic
AGGTGAAGATTATGCTTGAC+TGG 0.432560 1.1:+1572298 MS.gene27265:CDS
ACCAGAGAGCTTGCTGAAGA+TGG 0.438488 1.1:+1571631 MS.gene27265:CDS
GATGGCTATATGATCTCATC+TGG 0.452022 1.1:+1572112 MS.gene27265:CDS
TCTCTTCGTTACAAGCTTCT+CGG 0.453319 1.1:+1571962 MS.gene27265:CDS
ATGCCGAGAAGGTTAACAAC+AGG 0.456910 1.1:+1571912 MS.gene27265:CDS
CAAACAACAGGGACTGGGAC+AGG 0.457728 1.1:-1572441 None:intergenic
AGGAGGAGGAAGAGTACATC+AGG 0.461009 1.1:+1572385 MS.gene27265:CDS
TTGAGGTTTGTTATGGAAAG+TGG 0.475116 1.1:+1572019 MS.gene27265:CDS
GATTAGGTGAGAAGGGAAGG+AGG 0.484267 1.1:+1571825 MS.gene27265:intron
TATAGTTCAACACTGTTCTC+AGG 0.488325 1.1:-1571890 None:intergenic
GCCATCTTCAGCAAGCTCTC+TGG 0.491889 1.1:-1571632 None:intergenic
AGCCAAGTCCATGAAGTTCA+AGG 0.492737 1.1:+1572090 MS.gene27265:CDS
AATTAATGATTAGGTGAGAA+GGG 0.493314 1.1:+1571818 MS.gene27265:intron
TGTTATGGAAAGTGGTGCTA+AGG 0.499768 1.1:+1572027 MS.gene27265:CDS
TAGACATGTTCTTCTCAGAC+AGG 0.500852 1.1:+1572168 MS.gene27265:CDS
TTGTTGCAGTTCGTCGCTGA+CGG 0.501712 1.1:+1571577 MS.gene27265:intron
AATGATTAGGTGAGAAGGGA+AGG 0.513264 1.1:+1571822 MS.gene27265:intron
TGACCTCAGTAGTCCAGAAA+AGG 0.517945 1.1:+1571858 MS.gene27265:CDS
CTACGAACCAAGAACAGCTT+GGG 0.525454 1.1:-1571719 None:intergenic
GATGATGATCTCAGTGCGCA+TGG 0.527229 1.1:-1571683 None:intergenic
TCATTTGCAACAACAGCAGC+AGG 0.528096 1.1:-1572408 None:intergenic
TTTCTTCGCTGAATTGAATG+AGG 0.530015 1.1:+1571603 MS.gene27265:CDS
AATGGTAACAATATCAGGTA+GGG 0.532486 1.1:-1572352 None:intergenic
GCTGTTCGCAGAGCCTGTTA+CGG 0.553152 1.1:+1571992 MS.gene27265:CDS
GAGCCTGTTACGGTGTGTTG+AGG 0.556363 1.1:+1572002 MS.gene27265:CDS
ATGCTTGACTGGGATCCTAA+AGG 0.556783 1.1:+1572309 MS.gene27265:CDS
TGTTGTTGCAAATGATATTG+AGG 0.562213 1.1:+1572416 MS.gene27265:CDS
ACTACGAACCAAGAACAGCT+TGG 0.565206 1.1:-1571720 None:intergenic
TTTCTCAAACAACAGGGACT+GGG 0.568525 1.1:-1572446 None:intergenic
GTTATGGAAAGTGGTGCTAA+GGG 0.582081 1.1:+1572028 MS.gene27265:CDS
GAATGGTAACAATATCAGGT+AGG 0.585408 1.1:-1572353 None:intergenic
AGATGGCTATTCCGGTGTTG+AGG 0.587442 1.1:+1571648 MS.gene27265:CDS
GAATTAATGATTAGGTGAGA+AGG 0.589593 1.1:+1571817 MS.gene27265:intron
CAATGTAATCCTTAACAGGT+TGG 0.596885 1.1:-1572135 None:intergenic
TGGGATCCTAAAGGAAAGCA+AGG 0.601952 1.1:+1572318 MS.gene27265:CDS
GGTGAAGATTATGCTTGACT+GGG 0.603451 1.1:+1572299 MS.gene27265:CDS
AAACAACAGGGACTGGGACA+GGG 0.607283 1.1:-1572440 None:intergenic
TGTTGAACTATATGCCGAGA+AGG 0.615665 1.1:+1571901 MS.gene27265:CDS
TACCATTCACACTCCAAAGG+AGG 0.618529 1.1:+1572368 MS.gene27265:CDS
CATTCACACTCCAAAGGAGG+AGG 0.623248 1.1:+1572371 MS.gene27265:CDS
ATGGTAACAATATCAGGTAG+GGG 0.623679 1.1:-1572351 None:intergenic
TGTTACCATTCACACTCCAA+AGG 0.627045 1.1:+1572365 MS.gene27265:CDS
AAGTCCATGAAGTTCAAGGA+TGG 0.640546 1.1:+1572094 MS.gene27265:CDS
ATGATGATCTCAGTGCGCAT+GGG 0.667262 1.1:-1571682 None:intergenic
AAGTGGTGCTAAGGGATGTG+AGG 0.670962 1.1:+1572036 MS.gene27265:CDS
CTTCGTTACAAGCTTCTCGG+AGG 0.671532 1.1:+1571965 MS.gene27265:CDS
TGCCGAGAAGGTTAACAACA+GGG 0.674234 1.1:+1571913 MS.gene27265:CDS
AGTAACCCTAACCTCAACAC+CGG 0.681090 1.1:-1571659 None:intergenic
GCCGAGAAGGTTAACAACAG+GGG 0.682645 1.1:+1571914 MS.gene27265:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTATATGAATTTAAA+TGG + chr1.1:1571779-1571798 MS.gene27265:intron 10.0%
!! TGATTTATGAATTAATGATT+AGG + chr1.1:1571809-1571828 MS.gene27265:intron 15.0%
!!! ATTATTACCTTTATTTTGTA+GGG + chr1.1:1572261-1572280 MS.gene27265:intron 15.0%
!!! GATTATTACCTTTATTTTGT+AGG + chr1.1:1572260-1572279 MS.gene27265:intron 20.0%
! AATTAATGATTAGGTGAGAA+GGG + chr1.1:1571818-1571837 MS.gene27265:intron 25.0%
AAACCAACAAGGTTGTTATA+GGG - chr1.1:1572206-1572225 None:intergenic 30.0%
AAGAACACCCTACAAAATAA+AGG - chr1.1:1572271-1572290 None:intergenic 30.0%
AATAACAAACCAAACCAACA+AGG - chr1.1:1572217-1572236 None:intergenic 30.0%
AATGGTAACAATATCAGGTA+GGG - chr1.1:1572355-1572374 None:intergenic 30.0%
CACTACTAATAGAGATGAAT+TGG - chr1.1:1571516-1571535 None:intergenic 30.0%
GAATTAATGATTAGGTGAGA+AGG + chr1.1:1571817-1571836 MS.gene27265:intron 30.0%
TGTTGTTGCAAATGATATTG+AGG + chr1.1:1572416-1572435 MS.gene27265:CDS 30.0%
!!! TTTATTTTGTAGGGTGTTCT+TGG + chr1.1:1572270-1572289 MS.gene27265:intron 30.0%
ATGGTAACAATATCAGGTAG+GGG - chr1.1:1572354-1572373 None:intergenic 35.0%
CAAACCAACAAGGTTGTTAT+AGG - chr1.1:1572207-1572226 None:intergenic 35.0%
CAATGTAATCCTTAACAGGT+TGG - chr1.1:1572138-1572157 None:intergenic 35.0%
GAATGGTAACAATATCAGGT+AGG - chr1.1:1572356-1572375 None:intergenic 35.0%
GAGTCAATGTAATCCTTAAC+AGG - chr1.1:1572142-1572161 None:intergenic 35.0%
GTGTGAATGGTAACAATATC+AGG - chr1.1:1572360-1572379 None:intergenic 35.0%
TATAGTTCAACACTGTTCTC+AGG - chr1.1:1571893-1571912 None:intergenic 35.0%
TTACCCTATAACAACCTTGT+TGG + chr1.1:1572200-1572219 MS.gene27265:intron 35.0%
TTTCTTCGCTGAATTGAATG+AGG + chr1.1:1571603-1571622 MS.gene27265:CDS 35.0%
! CAGGAAACTTAAACCTTTTC+TGG - chr1.1:1571874-1571893 None:intergenic 35.0%
! CTATAACAACCTTGTTGGTT+TGG + chr1.1:1572205-1572224 MS.gene27265:intron 35.0%
!! TTGAGGTTTGTTATGGAAAG+TGG + chr1.1:1572019-1572038 MS.gene27265:CDS 35.0%
!!! CTTTCGCTTTTTGCTCATTT+GGG - chr1.1:1571465-1571484 None:intergenic 35.0%
AAGTCCATGAAGTTCAAGGA+TGG + chr1.1:1572094-1572113 MS.gene27265:CDS 40.0%
AATGATTAGGTGAGAAGGGA+AGG + chr1.1:1571822-1571841 MS.gene27265:intron 40.0%
AGGTGAAGATTATGCTTGAC+TGG + chr1.1:1572298-1572317 MS.gene27265:CDS 40.0%
ATAGCCATCCTTGAACTTCA+TGG - chr1.1:1572101-1572120 None:intergenic 40.0%
ATCCTTGAACTTCATGGACT+TGG - chr1.1:1572095-1572114 None:intergenic 40.0%
CCAAATGAGCAAAAAGCGAA+AGG + chr1.1:1571463-1571482 MS.gene27265:CDS 40.0%
GATGGCTATATGATCTCATC+TGG + chr1.1:1572112-1572131 MS.gene27265:CDS 40.0%
GGATGTGAGGTTATTGTTAG+TGG + chr1.1:1572049-1572068 MS.gene27265:CDS 40.0%
GGTGAAGATTATGCTTGACT+GGG + chr1.1:1572299-1572318 MS.gene27265:CDS 40.0%
TAGACATGTTCTTCTCAGAC+AGG + chr1.1:1572168-1572187 MS.gene27265:CDS 40.0%
TCTCTTCGTTACAAGCTTCT+CGG + chr1.1:1571962-1571981 MS.gene27265:CDS 40.0%
TGTTACCATTCACACTCCAA+AGG + chr1.1:1572365-1572384 MS.gene27265:CDS 40.0%
TGTTGAACTATATGCCGAGA+AGG + chr1.1:1571901-1571920 MS.gene27265:CDS 40.0%
TTAGGACCTTGCTTTCCTTT+AGG - chr1.1:1572327-1572346 None:intergenic 40.0%
! AAACCTTTTCTGGACTACTG+AGG - chr1.1:1571864-1571883 None:intergenic 40.0%
! ATATCAGGTAGGGGAGTTTT+AGG - chr1.1:1572345-1572364 None:intergenic 40.0%
! GTAGGGTGTTCTTGGTATTA+AGG + chr1.1:1572278-1572297 MS.gene27265:intron 40.0%
! GTTATGGAAAGTGGTGCTAA+GGG + chr1.1:1572028-1572047 MS.gene27265:CDS 40.0%
! TGTTATGGAAAGTGGTGCTA+AGG + chr1.1:1572027-1572046 MS.gene27265:CDS 40.0%
!!! CCTTTCGCTTTTTGCTCATT+TGG - chr1.1:1571466-1571485 None:intergenic 40.0%
AAACCTCAACACACCGTAAC+AGG - chr1.1:1572008-1572027 None:intergenic 45.0%
ACTACGAACCAAGAACAGCT+TGG - chr1.1:1571723-1571742 None:intergenic 45.0%
AGCCAAGTCCATGAAGTTCA+AGG + chr1.1:1572090-1572109 MS.gene27265:CDS 45.0%
AGTAACCCTAACCTCAACAC+CGG - chr1.1:1571662-1571681 None:intergenic 45.0%
ATGATGATCTCAGTGCGCAT+GGG - chr1.1:1571685-1571704 None:intergenic 45.0%
ATGCCGAGAAGGTTAACAAC+AGG + chr1.1:1571912-1571931 MS.gene27265:CDS 45.0%
ATGCTTGACTGGGATCCTAA+AGG + chr1.1:1572309-1572328 MS.gene27265:CDS 45.0%
ATGTACTCTTCCTCCTCCTT+TGG - chr1.1:1572384-1572403 None:intergenic 45.0%
CTACGAACCAAGAACAGCTT+GGG - chr1.1:1571722-1571741 None:intergenic 45.0%
CTTGCTGAAGATGGCTATTC+CGG + chr1.1:1571640-1571659 MS.gene27265:CDS 45.0%
TACCATTCACACTCCAAAGG+AGG + chr1.1:1572368-1572387 MS.gene27265:CDS 45.0%
TCATTTGCAACAACAGCAGC+AGG - chr1.1:1572411-1572430 None:intergenic 45.0%
TCCCCTGTTGTTAACCTTCT+CGG - chr1.1:1571918-1571937 None:intergenic 45.0%
TGACCTCAGTAGTCCAGAAA+AGG + chr1.1:1571858-1571877 MS.gene27265:CDS 45.0%
TGCCGAGAAGGTTAACAACA+GGG + chr1.1:1571913-1571932 MS.gene27265:CDS 45.0%
TGGGATCCTAAAGGAAAGCA+AGG + chr1.1:1572318-1572337 MS.gene27265:CDS 45.0%
! CTATTCCGGTGTTGAGGTTA+GGG + chr1.1:1571654-1571673 MS.gene27265:CDS 45.0%
!! CGGTGTGTTGAGGTTTGTTA+TGG + chr1.1:1572012-1572031 MS.gene27265:CDS 45.0%
AAACAACAGGGACTGGGACA+GGG - chr1.1:1572443-1572462 None:intergenic 50.0%
ACTCGTACCCAAGCTGTTCT+TGG + chr1.1:1571712-1571731 MS.gene27265:CDS 50.0%
CATTCACACTCCAAAGGAGG+AGG + chr1.1:1572371-1572390 MS.gene27265:CDS 50.0%
CTCATCTGGCCAACCTGTTA+AGG + chr1.1:1572126-1572145 MS.gene27265:CDS 50.0%
CTTCGTTACAAGCTTCTCGG+AGG + chr1.1:1571965-1571984 MS.gene27265:CDS 50.0%
GATGATGATCTCAGTGCGCA+TGG - chr1.1:1571686-1571705 None:intergenic 50.0%
GATTAGGTGAGAAGGGAAGG+AGG + chr1.1:1571825-1571844 MS.gene27265:intron 50.0%
GCCGAGAAGGTTAACAACAG+GGG + chr1.1:1571914-1571933 MS.gene27265:CDS 50.0%
TTGTTGCAGTTCGTCGCTGA+CGG + chr1.1:1571577-1571596 MS.gene27265:intron 50.0%
! AAGTGGTGCTAAGGGATGTG+AGG + chr1.1:1572036-1572055 MS.gene27265:CDS 50.0%
! AGATGGCTATTCCGGTGTTG+AGG + chr1.1:1571648-1571667 MS.gene27265:CDS 50.0%
! AGGAGGAGGAAGAGTACATC+AGG + chr1.1:1572385-1572404 MS.gene27265:CDS 50.0%
! GCTATTCCGGTGTTGAGGTT+AGG + chr1.1:1571653-1571672 MS.gene27265:CDS 50.0%
!! ACCAGAGAGCTTGCTGAAGA+TGG + chr1.1:1571631-1571650 MS.gene27265:CDS 50.0%
!! CTCCTCCTTTGGAGTGTGAA+TGG - chr1.1:1572373-1572392 None:intergenic 50.0%
CAAACAACAGGGACTGGGAC+AGG - chr1.1:1572444-1572463 None:intergenic 55.0%
GAGCCTGTTACGGTGTGTTG+AGG + chr1.1:1572002-1572021 MS.gene27265:CDS 55.0%
GCCATCTTCAGCAAGCTCTC+TGG - chr1.1:1571635-1571654 None:intergenic 55.0%
GCTGTTCGCAGAGCCTGTTA+CGG + chr1.1:1571992-1572011 MS.gene27265:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 1571455 1572464 1571455 ID=MS.gene27265
chr1.1 mRNA 1571455 1572464 1571455 ID=MS.gene27265.t1;Parent=MS.gene27265
chr1.1 exon 1571455 1571484 1571455 ID=MS.gene27265.t1.exon1;Parent=MS.gene27265.t1
chr1.1 CDS 1571455 1571484 1571455 ID=cds.MS.gene27265.t1;Parent=MS.gene27265.t1
chr1.1 exon 1571586 1571733 1571586 ID=MS.gene27265.t1.exon2;Parent=MS.gene27265.t1
chr1.1 CDS 1571586 1571733 1571586 ID=cds.MS.gene27265.t1;Parent=MS.gene27265.t1
chr1.1 exon 1571831 1572189 1571831 ID=MS.gene27265.t1.exon3;Parent=MS.gene27265.t1
chr1.1 CDS 1571831 1572189 1571831 ID=cds.MS.gene27265.t1;Parent=MS.gene27265.t1
chr1.1 exon 1572282 1572464 1572282 ID=MS.gene27265.t1.exon4;Parent=MS.gene27265.t1
chr1.1 CDS 1572282 1572464 1572282 ID=cds.MS.gene27265.t1;Parent=MS.gene27265.t1
Gene Sequence

>MS.gene27265

ATGGCTACCCAAATGAGCAAAAAGCGAAAGGTTTGTTTTCAGATTCGTTAATCGTAATCCAATTCATCTCTATTAGTAGTGATTTCTTAATAATTCTTAATTTGACAATTTGATTTGTGTTGTTGTTGCAGTTCGTCGCTGACGGAGTTTTCTTCGCTGAATTGAATGAGGTTTTAACCAGAGAGCTTGCTGAAGATGGCTATTCCGGTGTTGAGGTTAGGGTTACTCCCATGCGCACTGAGATCATCATCAGAGCTACTCGTACCCAAGCTGTTCTTGGTTCGTAGTCATTAATTTACAAATTAATCTGATTTTATTTATTTTTGTTTTTATATGAATTTAAATGGTTGAATCTGATTTATGAATTAATGATTAGGTGAGAAGGGAAGGAGGATCAGAGAACTGACCTCAGTAGTCCAGAAAAGGTTTAAGTTTCCTGAGAACAGTGTTGAACTATATGCCGAGAAGGTTAACAACAGGGGACTTTGTGCAATTGCACAAGCTGAGTCTCTTCGTTACAAGCTTCTCGGAGGTCTTGCTGTTCGCAGAGCCTGTTACGGTGTGTTGAGGTTTGTTATGGAAAGTGGTGCTAAGGGATGTGAGGTTATTGTTAGTGGAAAATTGAGAGCACAAAGAGCCAAGTCCATGAAGTTCAAGGATGGCTATATGATCTCATCTGGCCAACCTGTTAAGGATTACATTGACTCTGCTGTTAGACATGTTCTTCTCAGACAGGTTAGTTAGTTTACCCTATAACAACCTTGTTGGTTTGGTTTGTTATTGATTTATTTATGTTGCTAATGATGATTATTACCTTTATTTTGTAGGGTGTTCTTGGTATTAAGGTGAAGATTATGCTTGACTGGGATCCTAAAGGAAAGCAAGGTCCTAAAACTCCCCTACCTGATATTGTTACCATTCACACTCCAAAGGAGGAGGAAGAGTACATCAGGCCTGCTGCTGTTGTTGCAAATGATATTGAGGTCCCTGTCCCAGTCCCTGTTGTTTGA

Protein sequence

>MS.gene27265.t1

MATQMSKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQAVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMISSGQPVKDYIDSAVRHVLLRQGVLGIKVKIMLDWDPKGKQGPKTPLPDIVTIHTPKEEEEYIRPAAVVANDIEVPVPVPVV