AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028748

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028748.t1 MTR_2g093640 92.909 550 38 1 1 550 1 549 0.0 985
MS.gene028748.t1 MTR_2g093160 83.818 550 88 1 1 550 1 549 0.0 929
MS.gene028748.t1 MTR_2g093180 81.488 551 100 2 1 550 1 550 0.0 872
MS.gene028748.t1 MTR_4g107530 34.542 579 344 9 2 549 8 582 1.73e-110 342
MS.gene028748.t1 MTR_5g038060 34.281 563 343 8 9 548 19 577 8.86e-106 330
MS.gene028748.t1 MTR_4g107580 34.542 579 339 11 1 548 4 573 2.09e-105 329
MS.gene028748.t1 MTR_4g107510 34.983 586 326 12 8 550 13 586 2.90e-105 328
MS.gene028748.t1 MTR_8g469480 34.588 558 322 11 24 550 41 586 4.40e-101 318
MS.gene028748.t1 MTR_5g055000 33.454 553 339 10 20 550 17 562 5.52e-98 309
MS.gene028748.t1 MTR_2g101590 34.912 570 328 14 5 545 10 565 2.42e-97 309
MS.gene028748.t1 MTR_2g101650 35.336 566 327 13 13 550 14 568 3.34e-97 307
MS.gene028748.t1 MTR_3g112460 33.034 557 347 9 1 542 1 546 1.06e-96 305
MS.gene028748.t1 MTR_2g101610 34.622 569 329 14 6 545 12 566 3.88e-96 306
MS.gene028748.t1 MTR_7g088825 32.136 557 350 10 14 548 15 565 9.82e-96 304
MS.gene028748.t1 MTR_4g011280 33.925 563 335 13 9 545 18 569 1.38e-95 303
MS.gene028748.t1 MTR_4g107610 32.176 547 347 7 17 543 27 569 1.78e-95 303
MS.gene028748.t1 MTR_3g112470 33.214 557 337 10 2 542 9 546 3.96e-95 301
MS.gene028748.t1 MTR_1g026740 35.035 568 327 15 5 545 1 553 5.75e-95 301
MS.gene028748.t1 MTR_2g101640 33.972 574 338 14 1 545 1 562 2.18e-92 296
MS.gene028748.t1 MTR_1g057460 34.409 558 327 13 6 545 28 564 1.43e-91 292
MS.gene028748.t1 MTR_3g462710 31.729 561 339 12 17 549 20 564 6.01e-91 290
MS.gene028748.t1 MTR_7g088790 32.451 567 355 10 4 548 12 572 6.05e-91 291
MS.gene028748.t1 MTR_7g067010 31.162 568 358 9 2 550 10 563 3.33e-90 288
MS.gene028748.t1 MTR_7g088820 31.239 557 357 9 13 548 17 568 4.11e-90 289
MS.gene028748.t1 MTR_2g101560 33.803 568 335 12 6 545 12 566 2.85e-89 288
MS.gene028748.t1 MTR_1g026750 34.875 562 327 13 10 545 6 554 4.60e-89 285
MS.gene028748.t1 MTR_8g469470 32.136 557 346 11 20 549 31 582 8.51e-89 285
MS.gene028748.t1 MTR_5g038190 30.254 552 356 11 20 548 23 568 1.29e-88 284
MS.gene028748.t1 MTR_5g012270 32.072 555 341 10 21 549 29 573 1.58e-88 284
MS.gene028748.t1 MTR_8g103233 32.909 550 333 12 20 545 16 553 6.24e-87 280
MS.gene028748.t1 MTR_3g069500 32.228 543 340 10 21 550 35 562 7.83e-87 280
MS.gene028748.t1 MTR_3g112450 31.746 567 351 13 1 546 1 552 2.44e-85 276
MS.gene028748.t1 MTR_4g019580 32.014 556 343 10 18 550 28 571 8.30e-85 275
MS.gene028748.t1 MTR_7g065080 31.111 540 348 8 23 550 33 560 1.29e-84 274
MS.gene028748.t1 MTR_7g098220 31.424 576 347 12 2 550 16 570 1.31e-84 275
MS.gene028748.t1 MTR_7g098160 29.455 550 355 9 21 548 33 571 2.20e-84 274
MS.gene028748.t1 MTR_7g098090 30.090 555 350 10 21 548 33 576 2.28e-84 274
MS.gene028748.t1 MTR_4g101380 31.153 581 353 12 2 549 8 574 2.44e-84 273
MS.gene028748.t1 MTR_5g012290 32.270 564 345 13 10 549 21 571 4.56e-84 273
MS.gene028748.t1 MTR_7g098180 30.396 556 347 12 21 550 31 572 6.42e-84 273
MS.gene028748.t1 MTR_4g015080 29.893 562 348 9 17 550 30 573 6.65e-84 272
MS.gene028748.t1 MTR_4g114340 32.182 550 341 10 17 546 20 557 2.00e-83 271
MS.gene028748.t1 MTR_7g098200 30.055 549 351 9 23 549 31 568 2.86e-83 271
MS.gene028748.t1 MTR_7g098200 30.419 549 339 11 23 549 31 558 3.44e-83 271
MS.gene028748.t1 MTR_7g098040 28.909 550 358 11 21 548 34 572 3.72e-83 271
MS.gene028748.t1 MTR_1g057460 33.658 514 303 12 49 545 1 493 5.44e-83 268
MS.gene028748.t1 MTR_1g026770 32.743 565 341 13 5 545 1 550 8.14e-83 269
MS.gene028748.t1 MTR_1g009200 32.790 552 330 12 21 545 27 564 1.00e-82 269
MS.gene028748.t1 MTR_3g014790 31.004 558 328 9 22 549 18 548 2.33e-82 268
MS.gene028748.t1 MTR_1g042310 30.192 573 354 11 2 548 12 564 2.35e-82 268
MS.gene028748.t1 MTR_7g064780 31.993 572 351 13 8 549 6 569 2.85e-82 268
MS.gene028748.t1 MTR_7g098220 31.784 538 324 11 40 550 5 526 4.11e-82 266
MS.gene028748.t1 MTR_3g069440 30.909 550 348 8 21 550 33 570 8.25e-82 267
MS.gene028748.t1 MTR_1g075440 29.565 575 355 10 2 548 14 566 2.17e-81 266
MS.gene028748.t1 MTR_4g033325 34.470 557 304 15 21 550 31 553 4.87e-81 264
MS.gene028748.t1 MTR_7g098230 29.754 568 361 12 8 549 10 565 1.33e-80 264
MS.gene028748.t1 MTR_1g042310 30.467 535 331 10 40 548 8 527 2.39e-80 262
MS.gene028748.t1 MTR_1g042310 30.467 535 331 10 40 548 1 520 2.59e-80 262
MS.gene028748.t1 MTR_7g005930 29.137 556 371 8 6 546 20 567 9.32e-80 261
MS.gene028748.t1 MTR_8g038880 30.769 572 345 11 7 550 4 552 1.52e-79 260
MS.gene028748.t1 MTR_3g072300 30.420 572 355 11 2 549 10 562 3.06e-79 260
MS.gene028748.t1 MTR_7g088820 30.709 508 326 9 62 548 4 506 3.31e-79 258
MS.gene028748.t1 MTR_7g065080 31.612 484 307 8 79 550 2 473 1.02e-78 256
MS.gene028748.t1 MTR_7g064800 31.387 548 351 8 15 540 20 564 1.25e-77 256
MS.gene028748.t1 MTR_0236s0050 32.554 513 310 11 49 545 1 493 2.34e-77 253
MS.gene028748.t1 MTR_4g098800 30.784 536 340 9 39 549 54 583 5.40e-77 254
MS.gene028748.t1 MTR_0294s0020 31.818 572 330 12 1 549 16 550 6.75e-77 253
MS.gene028748.t1 MTR_4g098800 30.784 536 340 9 39 549 119 648 2.52e-76 254
MS.gene028748.t1 MTR_4g033315 31.818 572 332 14 1 549 16 552 3.32e-76 251
MS.gene028748.t1 MTR_4g033310 31.818 572 332 14 1 549 16 552 3.32e-76 251
MS.gene028748.t1 MTR_3g462710 32.409 469 273 12 109 549 3 455 6.43e-76 248
MS.gene028748.t1 MTR_5g014150 30.341 557 354 10 18 546 36 586 8.76e-76 251
MS.gene028748.t1 MTR_1g041875 28.625 531 339 9 21 529 29 541 2.71e-75 249
MS.gene028748.t1 MTR_1g041835 28.796 573 361 10 2 548 13 564 4.55e-75 249
MS.gene028748.t1 MTR_7g013270 29.358 545 360 8 19 546 88 624 5.18e-75 250
MS.gene028748.t1 MTR_1g041815 29.319 573 358 10 2 548 13 564 5.74e-75 249
MS.gene028748.t1 MTR_1g116930 30.127 551 358 11 17 550 19 559 9.53e-75 248
MS.gene028748.t1 MTR_4g033330 33.208 533 303 12 21 530 31 533 4.65e-74 245
MS.gene028748.t1 MTR_4g033395 33.075 517 289 12 1 497 17 496 1.31e-73 243
MS.gene028748.t1 MTR_4g033375 32.558 516 293 11 1 497 17 496 5.76e-73 241
MS.gene028748.t1 MTR_3g069420 30.631 555 339 10 23 550 36 571 3.48e-72 241
MS.gene028748.t1 MTR_7g088820 31.004 458 290 9 112 548 6 458 1.32e-71 237
MS.gene028748.t1 MTR_1g041885 27.668 506 342 8 40 531 1 496 1.63e-71 238
MS.gene028748.t1 MTR_3g005010 28.986 552 330 12 23 550 32 545 2.13e-70 236
MS.gene028748.t1 MTR_7g098200 30.238 463 290 9 109 549 3 454 7.16e-70 233
MS.gene028748.t1 MTR_5g085850 29.412 544 359 11 17 548 26 556 1.03e-69 235
MS.gene028748.t1 MTR_7g010820 31.215 535 334 9 39 547 44 570 2.37e-69 234
MS.gene028748.t1 MTR_2g005130 28.440 545 360 7 17 548 36 563 4.75e-69 233
MS.gene028748.t1 MTR_4g033325 34.615 442 240 13 136 550 10 429 1.39e-68 228
MS.gene028748.t1 MTR_4g033325 34.615 442 240 13 136 550 36 455 3.02e-68 228
MS.gene028748.t1 MTR_2g017750 29.128 539 351 11 39 548 52 588 3.05e-68 231
MS.gene028748.t1 MTR_7g098150 30.233 430 280 6 21 441 33 451 4.26e-68 228
MS.gene028748.t1 MTR_3g100980 27.419 558 358 13 20 550 15 552 2.80e-67 228
MS.gene028748.t1 MTR_5g038380 27.385 566 354 15 21 548 28 574 5.48e-67 228
MS.gene028748.t1 MTR_7g111260 28.051 549 357 13 20 549 18 547 6.05e-65 222
MS.gene028748.t1 MTR_4g033385 32.812 448 252 12 129 549 28 453 7.19e-65 219
MS.gene028748.t1 MTR_8g469310 27.500 560 360 16 21 548 26 571 5.06e-64 219
MS.gene028748.t1 MTR_4g123537 28.169 568 370 14 2 549 22 571 7.57e-62 214
MS.gene028748.t1 MTR_3g100980 25.812 585 361 13 20 550 15 580 2.72e-61 213
MS.gene028748.t1 MTR_2g093650 58.511 188 35 2 339 526 34 178 2.75e-61 201
MS.gene028748.t1 MTR_4g107670 26.538 569 356 15 22 549 25 572 1.02e-60 211
MS.gene028748.t1 MTR_1g085090 26.064 564 373 14 10 549 4 547 1.66e-60 211
MS.gene028748.t1 MTR_8g087780 26.562 576 388 10 1 548 6 574 2.59e-59 207
MS.gene028748.t1 MTR_8g091265 72.519 131 31 2 290 415 3 133 2.22e-58 191
MS.gene028748.t1 MTR_7g006540 26.596 564 378 12 14 548 56 612 1.09e-57 204
MS.gene028748.t1 MTR_8g087810 27.383 577 383 11 1 548 6 575 1.90e-57 202
MS.gene028748.t1 MTR_6g008690 26.087 552 334 14 34 546 19 535 5.78e-50 181
MS.gene028748.t1 MTR_4g024930 25.688 545 359 12 21 546 36 553 1.78e-49 180
MS.gene028748.t1 MTR_7g051830 24.731 558 363 13 2 545 11 525 2.16e-49 179
MS.gene028748.t1 MTR_7g098210 26.705 528 276 12 44 549 5 443 1.12e-48 175
MS.gene028748.t1 MTR_7g052600 25.045 559 360 16 2 545 11 525 1.50e-48 176
MS.gene028748.t1 MTR_7g051880 24.014 558 367 14 2 545 11 525 6.84e-47 172
MS.gene028748.t1 MTR_1g009170 29.000 400 213 11 149 528 9 357 1.36e-37 143
MS.gene028748.t1 MTR_4g033310 28.076 317 194 8 249 549 190 488 4.42e-32 129
MS.gene028748.t1 MTR_4g033310 34.536 194 105 5 1 189 16 192 1.28e-17 86.3
MS.gene028748.t1 MTR_8g091275 59.722 72 28 1 197 267 1 72 9.31e-25 98.6
MS.gene028748.t1 MTR_8g091480 60.870 69 21 2 221 289 27 89 1.68e-16 83.6
MS.gene028748.t1 MTR_3g065350 32.692 156 95 5 331 480 1 152 2.12e-12 65.9
MS.gene028748.t1 MTR_7g064160 31.915 94 63 1 15 107 23 116 8.84e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028748.t1 AT3G45660 49.551 557 261 4 1 550 1 544 0.0 559
MS.gene028748.t1 AT3G45660 49.551 557 261 4 1 550 1 544 0.0 559
MS.gene028748.t1 AT3G45660 49.551 557 261 4 1 550 22 565 0.0 558
MS.gene028748.t1 AT3G45650 48.925 558 264 5 1 550 1 545 0.0 550
MS.gene028748.t1 AT3G45680 49.270 548 273 3 3 550 2 544 0.0 542
MS.gene028748.t1 AT3G45710 47.723 549 277 3 2 550 8 546 0.0 540
MS.gene028748.t1 AT3G45650 46.747 584 264 6 1 550 1 571 0.0 536
MS.gene028748.t1 AT3G45700 46.875 544 278 4 7 550 2 534 0.0 521
MS.gene028748.t1 AT3G45720 45.818 550 290 4 1 550 1 542 9.77e-175 505
MS.gene028748.t1 AT3G45690 45.055 546 242 7 11 550 8 501 1.74e-161 470
MS.gene028748.t1 AT1G69870 34.909 550 332 10 20 548 50 594 2.85e-109 339
MS.gene028748.t1 AT1G18880 35.602 573 320 10 11 550 12 568 2.56e-107 333
MS.gene028748.t1 AT3G47960 35.856 555 316 11 24 548 64 608 2.64e-103 324
MS.gene028748.t1 AT5G62680 34.155 568 334 11 13 548 33 592 1.32e-99 314
MS.gene028748.t1 AT1G27080 32.781 543 344 8 20 548 13 548 6.86e-98 308
MS.gene028748.t1 AT3G16180 33.569 566 339 13 8 545 9 565 1.15e-94 301
MS.gene028748.t1 AT2G26690 33.394 554 337 10 10 546 18 556 1.06e-93 298
MS.gene028748.t1 AT1G68570 33.624 574 353 10 1 550 1 570 1.49e-93 298
MS.gene028748.t1 AT1G52190 32.570 568 346 13 8 545 6 566 3.05e-93 297
MS.gene028748.t1 AT1G62200 33.761 545 324 11 20 541 51 581 3.10e-92 294
MS.gene028748.t1 AT1G27080 32.749 513 324 8 50 548 1 506 1.01e-91 291
MS.gene028748.t1 AT3G54140 30.490 551 357 9 17 548 20 563 1.07e-90 290
MS.gene028748.t1 AT3G54140 30.490 551 357 9 17 548 20 563 1.07e-90 290
MS.gene028748.t1 AT2G26690 33.588 524 321 9 40 546 10 523 7.10e-90 286
MS.gene028748.t1 AT5G01180 31.284 553 353 9 17 550 21 565 1.14e-89 287
MS.gene028748.t1 AT5G01180 31.284 553 353 9 17 550 21 565 1.14e-89 287
MS.gene028748.t1 AT5G01180 31.284 553 353 9 17 550 21 565 1.14e-89 287
MS.gene028748.t1 AT1G62200 32.923 568 327 12 20 541 51 610 2.02e-89 288
MS.gene028748.t1 AT2G26690 33.654 520 318 9 44 546 2 511 2.03e-89 285
MS.gene028748.t1 AT1G62200 32.923 568 327 12 20 541 65 624 2.83e-89 288
MS.gene028748.t1 AT1G68570 34.270 534 325 9 39 550 11 540 4.27e-89 285
MS.gene028748.t1 AT3G54450 29.307 563 347 8 15 550 6 544 5.35e-89 285
MS.gene028748.t1 AT1G69860 31.383 564 358 11 1 549 1 550 2.68e-83 270
MS.gene028748.t1 AT5G28470 30.474 548 357 11 20 549 16 557 1.65e-82 268
MS.gene028748.t1 AT5G46040 31.115 556 327 12 9 529 11 545 3.27e-82 268
MS.gene028748.t1 AT5G28470 30.220 546 359 10 20 549 16 555 6.08e-82 266
MS.gene028748.t1 AT3G53960 29.965 564 368 11 5 550 21 575 6.46e-80 262
MS.gene028748.t1 AT2G02020 30.380 553 315 10 20 550 41 545 7.52e-80 260
MS.gene028748.t1 AT2G40460 31.720 558 327 13 21 549 24 556 1.02e-79 261
MS.gene028748.t1 AT2G02040 29.891 552 356 10 20 549 40 582 1.23e-78 258
MS.gene028748.t1 AT2G37900 30.700 557 355 12 13 550 24 568 1.72e-77 255
MS.gene028748.t1 AT3G21670 30.642 545 346 13 21 548 29 558 1.22e-75 251
MS.gene028748.t1 AT5G46050 28.807 545 349 12 2 524 13 540 1.27e-72 243
MS.gene028748.t1 AT1G72125 30.961 562 326 14 18 550 30 558 1.83e-72 241
MS.gene028748.t1 AT5G19640 28.598 535 360 9 22 546 60 582 2.48e-72 242
MS.gene028748.t1 AT1G27040 28.596 570 370 11 1 547 1 556 2.97e-72 241
MS.gene028748.t1 AT1G27040 28.596 570 370 11 1 547 5 560 3.57e-72 241
MS.gene028748.t1 AT1G32450 30.263 532 343 11 39 546 55 582 1.06e-71 241
MS.gene028748.t1 AT2G37900 30.346 491 317 9 73 550 11 489 1.55e-71 237
MS.gene028748.t1 AT1G27040 29.333 525 330 11 47 547 21 528 3.21e-71 238
MS.gene028748.t1 AT1G12110 30.018 563 358 10 10 549 21 570 1.37e-70 237
MS.gene028748.t1 AT4G21680 28.623 552 358 10 23 547 30 572 1.97e-69 234
MS.gene028748.t1 AT1G33440 29.282 543 359 8 21 548 38 570 4.35e-69 233
MS.gene028748.t1 AT1G69850 29.930 568 349 16 21 550 25 581 3.18e-67 228
MS.gene028748.t1 AT1G22540 31.569 548 320 12 23 545 32 549 3.55e-67 227
MS.gene028748.t1 AT1G72140 28.136 558 330 11 23 549 36 553 5.54e-67 227
MS.gene028748.t1 AT1G22550 29.295 553 343 10 23 550 32 561 7.98e-66 224
MS.gene028748.t1 AT1G59740 28.440 545 361 8 17 542 37 571 2.70e-65 223
MS.gene028748.t1 AT1G72130 29.520 542 329 9 23 547 27 532 5.94e-65 221
MS.gene028748.t1 AT1G22570 29.887 532 320 12 23 526 32 538 1.23e-64 221
MS.gene028748.t1 AT1G72120 30.144 554 331 10 23 550 31 554 8.65e-64 218
MS.gene028748.t1 AT2G02020 29.508 549 304 14 20 550 41 524 1.58e-62 214
MS.gene028748.t1 AT5G46050 29.680 438 276 9 108 524 2 428 1.58e-62 213
MS.gene028748.t1 AT1G22570 29.254 523 317 12 32 526 4 501 2.66e-61 211
MS.gene028748.t1 AT3G25260 28.770 504 317 8 48 545 48 515 1.82e-60 208
MS.gene028748.t1 AT5G14940 26.889 569 348 11 16 546 1 539 1.30e-55 196
MS.gene028748.t1 AT5G62730 26.439 556 343 13 27 549 5 527 2.04e-55 195
MS.gene028748.t1 AT1G72130 29.861 432 261 8 133 547 8 414 2.60e-55 192
MS.gene028748.t1 AT1G72130 29.861 432 261 8 133 547 8 414 2.60e-55 192
MS.gene028748.t1 AT5G62730 26.418 564 347 14 21 549 56 586 2.69e-55 196
MS.gene028748.t1 AT5G11570 27.140 549 309 13 13 545 3 476 9.06e-55 192
MS.gene028748.t1 AT5G13400 25.487 565 385 10 14 550 57 613 1.51e-54 195
MS.gene028748.t1 AT3G25280 26.145 524 337 12 34 545 36 521 9.89e-48 174
MS.gene028748.t1 AT3G01350 25.532 564 336 12 34 546 19 549 5.72e-46 170
MS.gene028748.t1 AT2G38100 25.617 527 326 13 49 549 2 488 3.23e-35 139
MS.gene028748.t1 AT2G38100 25.617 527 326 13 49 549 18 504 5.97e-35 138
MS.gene028748.t1 AT2G38100 25.617 527 326 13 49 549 24 510 6.77e-35 138

Find 112 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTACATCGAAGATAATT+TGG 0.115376 2.3:+8228474 MS.gene028748:CDS
GCTGCATGGATGAGGCTTTA+AGG 0.172746 2.3:-8228236 None:intergenic
GCCACAGGGAAGTGCGATTT+TGG 0.172832 2.3:+8228588 MS.gene028748:CDS
ATCGTTGCTGATTCTTTCTT+TGG 0.187254 2.3:+8227784 MS.gene028748:CDS
GCAGCGGTTTCAATGCTTAT+AGG 0.248050 2.3:+8229691 MS.gene028748:CDS
TTCACTTTCTATGTTGCTTC+AGG 0.254353 2.3:+8228433 MS.gene028748:CDS
CCAGGTTGGCATAAGGTTAC+TGG 0.278137 2.3:+8229424 MS.gene028748:CDS
GATAATTTGGGTTGGGCTAT+AGG 0.307130 2.3:+8228487 MS.gene028748:CDS
GTCAAGAAAAGTGAGGAAAA+TGG 0.307219 2.3:-8229389 None:intergenic
AAGTCCATTTGCATTACTAT+TGG 0.312103 2.3:-8229271 None:intergenic
GATTACTACGGTAGTTCTTC+TGG 0.315848 2.3:+8228676 MS.gene028748:CDS
TTGGGTTGGGCTATAGGATT+TGG 0.320970 2.3:+8228493 MS.gene028748:CDS
CAATTAGTGTTGGTTGGTAT+TGG 0.327167 2.3:+8229592 MS.gene028748:CDS
TGTGGCTGCCTTTGTTGGTT+TGG 0.335104 2.3:+8228525 MS.gene028748:CDS
TTATTAATATCAATTGGATT+TGG 0.338772 2.3:+8228325 MS.gene028748:CDS
ATCTTCGATGTAAATAATTC+CGG 0.340345 2.3:-8228467 None:intergenic
ATAAGTTTCCCCTTCTTCAT+TGG 0.341967 2.3:+8227474 MS.gene028748:CDS
CAGCGGTTTCAATGCTTATA+GGG 0.345560 2.3:+8229692 MS.gene028748:CDS
CCTAAGCTGCTCCAACAAAT+AGG 0.348313 2.3:+8229454 MS.gene028748:CDS
AGCATATTATTAATATCAAT+TGG 0.361099 2.3:+8228319 MS.gene028748:CDS
CAATACCGGGAAAAGGATTA+AGG 0.369577 2.3:+8228731 MS.gene028748:CDS
GCATTACTATTGGAATTGAT+AGG 0.375721 2.3:-8229261 None:intergenic
CTTTAATTGACCAAGTTCAT+AGG 0.376672 2.3:+8229737 MS.gene028748:CDS
ATTTACATCGAAGATAATTT+GGG 0.382217 2.3:+8228475 MS.gene028748:CDS
ATGTTTCAATGTCTGTGTTA+TGG 0.382577 2.3:+8229560 MS.gene028748:CDS
TTAGTGATGGATCGTTCATT+AGG 0.387822 2.3:+8229310 MS.gene028748:CDS
TGTGCTGTGGCTGCCTTTGT+TGG 0.388715 2.3:+8228520 MS.gene028748:CDS
GCAACGATTGCGGCCACTAC+TGG 0.405757 2.3:-8227769 None:intergenic
CATCGAAGATAATTTGGGTT+GGG 0.406002 2.3:+8228480 MS.gene028748:CDS
CACAATTCTATTATTCTTGA+CGG 0.406311 2.3:+8228201 MS.gene028748:CDS
TACTGGATGTTAGTCGTACT+CGG 0.406589 2.3:+8229811 MS.gene028748:CDS
TTCCCGTGATTTATGTCATC+AGG 0.412476 2.3:-8229775 None:intergenic
TAATTGGAATATTACCACTA+TGG 0.412509 2.3:+8229224 MS.gene028748:CDS
GCTTCAGGAGCTGCATTAAC+CGG 0.417423 2.3:+8228448 MS.gene028748:CDS
CATGTCCTACTCCTATTTGT+TGG 0.420471 2.3:-8229465 None:intergenic
CCTATTTGTTGGAGCAGCTT+AGG 0.424589 2.3:-8229454 None:intergenic
TGTTGGTGGTTTGCATGTGA+TGG 0.432040 2.3:-8228269 None:intergenic
AGTGCTGCCTCCGCCAATAC+CGG 0.445352 2.3:+8228717 MS.gene028748:CDS
TTAATATCAATTGGATTTGG+TGG 0.448126 2.3:+8228328 MS.gene028748:CDS
AAGTTCATAGGAGTACTAAT+TGG 0.451212 2.3:+8229749 MS.gene028748:CDS
TCAAGAAAAGTGAGGAAAAT+GGG 0.451760 2.3:-8229388 None:intergenic
CAAATTTATAATGTTGTCGT+TGG 0.452697 2.3:+8227730 MS.gene028748:CDS
TAATAGAATGTAACTTGTCC+TGG 0.454723 2.3:-8229631 None:intergenic
AATTCCAATAGTAATGCAAA+TGG 0.460508 2.3:+8229267 MS.gene028748:CDS
ACTTGCAAGCAATGGAATAG+TGG 0.463177 2.3:+8227651 MS.gene028748:CDS
GTGCGCAACAATGGATGGTC+AGG 0.463595 2.3:+8227389 None:intergenic
GGCCACTACTGGAAATATAC+TGG 0.468506 2.3:-8227758 None:intergenic
GGACATGTGCTGAATGTTCT+TGG 0.468739 2.3:+8229481 MS.gene028748:CDS
CTTGACAGGATGCTATTGCC+AGG 0.474278 2.3:+8229406 MS.gene028748:CDS
CCAGTAACCTTATGCCAACC+TGG 0.475202 2.3:-8229424 None:intergenic
TTGTTGCATCTGCTCGTAAG+TGG 0.481137 2.3:+8228626 MS.gene028748:CDS
TTTCCACAATTAGTGTTGGT+TGG 0.482453 2.3:+8229586 MS.gene028748:CDS
AGAGAGAGACCAATGAAGAA+GGG 0.482917 2.3:-8227483 None:intergenic
GCTATTGCCAGGTTGGCATA+AGG 0.485413 2.3:+8229417 MS.gene028748:CDS
AAGAGAGAGACCAATGAAGA+AGG 0.489487 2.3:-8227484 None:intergenic
ACTGTGTATTGGATTCTTGT+TGG 0.495208 2.3:-8228286 None:intergenic
ATGGAAGAAAGCGCGGCGGT+TGG 0.496987 2.3:+8227451 MS.gene028748:CDS
CCTCCGCCAATACCGGGAAA+AGG 0.506457 2.3:+8228724 MS.gene028748:CDS
TTATGTATAAAACTGTGTAT+TGG 0.509435 2.3:-8228297 None:intergenic
GTGTATTGGATTCTTGTTGG+TGG 0.509836 2.3:-8228283 None:intergenic
ACATCGAAGATAATTTGGGT+TGG 0.520616 2.3:+8228479 MS.gene028748:CDS
CGTTTCACAGCAGCATCACT+AGG 0.522507 2.3:+8228355 MS.gene028748:CDS
GTGCTGCCTCCGCCAATACC+GGG 0.523813 2.3:+8228718 MS.gene028748:CDS
TATACTTCAAGCTTTAGTGA+TGG 0.526822 2.3:+8229297 MS.gene028748:CDS
GCAGATGAGAGCTTGAGTAA+TGG 0.528220 2.3:+8227432 MS.gene028748:CDS
ACAGGATGCTATTGCCAGGT+TGG 0.530502 2.3:+8229410 MS.gene028748:CDS
AATTAGTACTCCTATGAACT+TGG 0.530646 2.3:-8229747 None:intergenic
GTTGTTTCCACAATTAGTGT+TGG 0.534629 2.3:+8229582 MS.gene028748:CDS
ATACCAACCAACACTAATTG+TGG 0.537554 2.3:-8229589 None:intergenic
TTAACTATAGGTATGATAGC+GGG 0.538516 2.3:+8227622 MS.gene028748:intron
CTTGCAAGCAATGGAATAGT+GGG 0.541989 2.3:+8227652 MS.gene028748:CDS
TTATCGGGCTGAAAAGCCAC+AGG 0.543586 2.3:+8228573 MS.gene028748:CDS
GAGAGAGACCAATGAAGAAG+GGG 0.547308 2.3:-8227482 None:intergenic
GGAAGATCGACAATGTGTAC+TGG 0.551643 2.3:+8229794 MS.gene028748:CDS
CACCAGTATATTTCCAGTAG+TGG 0.555389 2.3:+8227756 MS.gene028748:CDS
ACTGGATGTTAGTCGTACTC+GGG 0.556684 2.3:+8229812 MS.gene028748:CDS
GAACCATTGCTGCATGGATG+AGG 0.561384 2.3:-8228244 None:intergenic
ATAACAGAAATAGAACCAGC+TGG 0.562257 2.3:-8229349 None:intergenic
GGGATGTCACTTGCAAGCAA+TGG 0.563554 2.3:+8227643 MS.gene028748:CDS
GCGAAGTGCGCAACAATGGA+TGG 0.567732 2.3:+8227384 None:intergenic
CTACCTGATGACATAAATCA+CGG 0.570213 2.3:+8229772 MS.gene028748:CDS
ATTAACTATAGGTATGATAG+CGG 0.570505 2.3:+8227621 MS.gene028748:intron
ACCGTAGTAATCCTCTACTC+TGG 0.578927 2.3:-8228665 None:intergenic
GCACAATTAAGAAATCATCA+TGG 0.579981 2.3:+8229161 MS.gene028748:CDS
TAAAATACAAGAAGAAGCAA+AGG 0.580215 2.3:-8227821 None:intergenic
CTGGATGTTAGTCGTACTCG+GGG 0.588713 2.3:+8229813 MS.gene028748:CDS
CTGCTCCAACAAATAGGAGT+AGG 0.598863 2.3:+8229460 MS.gene028748:CDS
TGTGCTGAATGTTCTTGGCA+TGG 0.599136 2.3:+8229486 MS.gene028748:CDS
ACTATGCACAATTCAACAAG+TGG 0.600830 2.3:+8229186 MS.gene028748:CDS
AAGCCTCATCCATGCAGCAA+TGG 0.601337 2.3:+8228241 MS.gene028748:CDS
GCTGAATGTTCTTGGCATGG+TGG 0.607060 2.3:+8229489 MS.gene028748:CDS
GAAAGAATCAGCAACGATTG+CGG 0.609321 2.3:-8227779 None:intergenic
TAACTATAGGTATGATAGCG+GGG 0.610759 2.3:+8227623 MS.gene028748:intron
TAAGCTCTCTTCCAGAGTAG+AGG 0.612591 2.3:+8228654 MS.gene028748:CDS
TTTGACAAACCAGAACATCA+AGG 0.615786 2.3:+8228388 MS.gene028748:CDS
TCCAAAATCGCACTTCCCTG+TGG 0.616079 2.3:-8228589 None:intergenic
AATAGACTTGAAGTCCATAG+TGG 0.622304 2.3:-8229238 None:intergenic
TAACAGAAATAGAACCAGCT+GGG 0.623287 2.3:-8229348 None:intergenic
GATGCAGATCTACACTCAGA+TGG 0.624333 2.3:+8229139 MS.gene028748:CDS
AGGATTTGGTATCTGTGCTG+TGG 0.626074 2.3:+8228507 MS.gene028748:CDS
TACCTGATGACATAAATCAC+GGG 0.627724 2.3:+8229773 MS.gene028748:CDS
TCCAGAGTAGAGGATTACTA+CGG 0.636666 2.3:+8228664 MS.gene028748:CDS
GATGTGCTCTTTAGTGATTG+TGG 0.636964 2.3:-8229664 None:intergenic
ATGGATGAACCATTGCTGCA+TGG 0.645148 2.3:-8228250 None:intergenic
CATCAGTTATTAGAGCTGCA+CGG 0.649496 2.3:-8229120 None:intergenic
TTGCATCTGCTCGTAAGTGG+AGG 0.661442 2.3:+8228629 MS.gene028748:CDS
TTGAGTAATGGAAGAAAGCG+CGG 0.673268 2.3:+8227444 MS.gene028748:CDS
AAAGAGCACATCAACTGCAG+CGG 0.686439 2.3:+8229675 MS.gene028748:CDS
GCATCCTGTCAAGAAAAGTG+AGG 0.686991 2.3:-8229396 None:intergenic
TATCGGGCTGAAAAGCCACA+GGG 0.693905 2.3:+8228574 MS.gene028748:CDS
TGGATGTTAGTCGTACTCGG+GGG 0.758348 2.3:+8229814 MS.gene028748:CDS
AGTAATGGAAGAAAGCGCGG+CGG 0.807785 2.3:+8227447 MS.gene028748:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATATTTACATCTAATA+TGG + chr2.3:8227937-8227956 MS.gene028748:intron 10.0%
!! AGCATATTATTAATATCAAT+TGG + chr2.3:8228319-8228338 MS.gene028748:CDS 15.0%
!! GAAGATTTCAAAAAAATAAT+TGG + chr2.3:8229208-8229227 MS.gene028748:CDS 15.0%
!! TTATTAATATCAATTGGATT+TGG + chr2.3:8228325-8228344 MS.gene028748:CDS 15.0%
!! ATATTGTAAAATAGGACATT+TGG - chr2.3:8228103-8228122 None:intergenic 20.0%
!! ATTTACATCGAAGATAATTT+GGG + chr2.3:8228475-8228494 MS.gene028748:CDS 20.0%
!! TATTTACATCGAAGATAATT+TGG + chr2.3:8228474-8228493 MS.gene028748:CDS 20.0%
!! TGATGAGTTAAAAAATGAAA+GGG - chr2.3:8227513-8227532 None:intergenic 20.0%
!! TTATGTATAAAACTGTGTAT+TGG - chr2.3:8228300-8228319 None:intergenic 20.0%
!! TTGATGAGTTAAAAAATGAA+AGG - chr2.3:8227514-8227533 None:intergenic 20.0%
!!! TTATACTGTTGATTAACTAT+AGG + chr2.3:8227610-8227629 MS.gene028748:intron 20.0%
!!! TTTGTTTACTTTTTTCAAGT+TGG + chr2.3:8228045-8228064 MS.gene028748:intron 20.0%
! AAAAATATTCCTTGATGTTC+TGG - chr2.3:8228400-8228419 None:intergenic 25.0%
! AACTTTACAAGCATATTCTT+TGG - chr2.3:8229053-8229072 None:intergenic 25.0%
! AATAAGATTATCTGCATTCA+AGG - chr2.3:8228812-8228831 None:intergenic 25.0%
! AATTCCAATAGTAATGCAAA+TGG + chr2.3:8229267-8229286 MS.gene028748:CDS 25.0%
! AGCATATATGCAACATAATT+TGG - chr2.3:8227872-8227891 None:intergenic 25.0%
! ATCTTCGATGTAAATAATTC+CGG - chr2.3:8228470-8228489 None:intergenic 25.0%
! ATTAACTATAGGTATGATAG+CGG + chr2.3:8227621-8227640 MS.gene028748:intron 25.0%
! ATTCTCATAGTTCAAATGTA+GGG + chr2.3:8228775-8228794 MS.gene028748:intron 25.0%
! CAAATTTATAATGTTGTCGT+TGG + chr2.3:8227730-8227749 MS.gene028748:CDS 25.0%
! CACAATTCTATTATTCTTGA+CGG + chr2.3:8228201-8228220 MS.gene028748:CDS 25.0%
! CTAAAAAAAGAACGTAATAG+AGG - chr2.3:8227996-8228015 None:intergenic 25.0%
! TAAAATACAAGAAGAAGCAA+AGG - chr2.3:8227824-8227843 None:intergenic 25.0%
! TAATTGGAATATTACCACTA+TGG + chr2.3:8229224-8229243 MS.gene028748:CDS 25.0%
! TATTCTCATAGTTCAAATGT+AGG + chr2.3:8228774-8228793 MS.gene028748:intron 25.0%
! TGCACATCATATTGTAAAAT+AGG - chr2.3:8228111-8228130 None:intergenic 25.0%
! TTAATATCAATTGGATTTGG+TGG + chr2.3:8228328-8228347 MS.gene028748:CDS 25.0%
!! TTCTTCTTGTATTTTATTGC+TGG + chr2.3:8227828-8227847 MS.gene028748:CDS 25.0%
!!! ATCAAGGAATATTTTTCAAC+TGG + chr2.3:8228404-8228423 MS.gene028748:CDS 25.0%
!!! TTACGTTCTTTTTTTAGAAG+TGG + chr2.3:8227999-8228018 MS.gene028748:intron 25.0%
AAAAACAACCAAACCAACAA+AGG - chr2.3:8228536-8228555 None:intergenic 30.0%
AAATATGTGATGACTGATGA+TGG - chr2.3:8227973-8227992 None:intergenic 30.0%
AAATGATAGCTGACAGTTTA+GGG + chr2.3:8228863-8228882 MS.gene028748:intron 30.0%
AAGTCCATTTGCATTACTAT+TGG - chr2.3:8229274-8229293 None:intergenic 30.0%
AATAGACATCCTAACAATGT+TGG - chr2.3:8228891-8228910 None:intergenic 30.0%
AATTAGTACTCCTATGAACT+TGG - chr2.3:8229750-8229769 None:intergenic 30.0%
ACCAAACATACACTTATTTG+TGG + chr2.3:8228139-8228158 MS.gene028748:intron 30.0%
ACCACAAATAAGTGTATGTT+TGG - chr2.3:8228143-8228162 None:intergenic 30.0%
ATGTTTCAATGTCTGTGTTA+TGG + chr2.3:8229560-8229579 MS.gene028748:CDS 30.0%
CTTTAATTGACCAAGTTCAT+AGG + chr2.3:8229737-8229756 MS.gene028748:CDS 30.0%
GCACAATTAAGAAATCATCA+TGG + chr2.3:8229161-8229180 MS.gene028748:CDS 30.0%
GCATTACTATTGGAATTGAT+AGG - chr2.3:8229264-8229283 None:intergenic 30.0%
TCAAGAAAAGTGAGGAAAAT+GGG - chr2.3:8229391-8229410 None:intergenic 30.0%
TTAACTATAGGTATGATAGC+GGG + chr2.3:8227622-8227641 MS.gene028748:intron 30.0%
TTCTCATAGTTCAAATGTAG+GGG + chr2.3:8228776-8228795 MS.gene028748:intron 30.0%
TTGAACTATGAGAATAAGTG+TGG - chr2.3:8228770-8228789 None:intergenic 30.0%
! TAATAGAATGTAACTTGTCC+TGG - chr2.3:8229634-8229653 None:intergenic 30.0%
! TATACTTCAAGCTTTAGTGA+TGG + chr2.3:8229297-8229316 MS.gene028748:CDS 30.0%
!! AAGTTCATAGGAGTACTAAT+TGG + chr2.3:8229749-8229768 MS.gene028748:CDS 30.0%
!!! TGTTGGGTTATCGATTTTAT+CGG + chr2.3:8228557-8228576 MS.gene028748:CDS 30.0%
AATAGACTTGAAGTCCATAG+TGG - chr2.3:8229241-8229260 None:intergenic 35.0%
AATGATAGCTGACAGTTTAG+GGG + chr2.3:8228864-8228883 MS.gene028748:intron 35.0%
ACATCGAAGATAATTTGGGT+TGG + chr2.3:8228479-8228498 MS.gene028748:CDS 35.0%
ACTATGCACAATTCAACAAG+TGG + chr2.3:8229186-8229205 MS.gene028748:CDS 35.0%
ACTGTGTATTGGATTCTTGT+TGG - chr2.3:8228289-8228308 None:intergenic 35.0%
ATAACAGAAATAGAACCAGC+TGG - chr2.3:8229352-8229371 None:intergenic 35.0%
ATAAGTTTCCCCTTCTTCAT+TGG + chr2.3:8227474-8227493 MS.gene028748:CDS 35.0%
ATACCAACCAACACTAATTG+TGG - chr2.3:8229592-8229611 None:intergenic 35.0%
CAAATGATAGCTGACAGTTT+AGG + chr2.3:8228862-8228881 MS.gene028748:intron 35.0%
CTACCTGATGACATAAATCA+CGG + chr2.3:8229772-8229791 MS.gene028748:CDS 35.0%
GTCAAGAAAAGTGAGGAAAA+TGG - chr2.3:8229392-8229411 None:intergenic 35.0%
TAACAGAAATAGAACCAGCT+GGG - chr2.3:8229351-8229370 None:intergenic 35.0%
TACCTGATGACATAAATCAC+GGG + chr2.3:8229773-8229792 MS.gene028748:CDS 35.0%
TTTGACAAACCAGAACATCA+AGG + chr2.3:8228388-8228407 MS.gene028748:CDS 35.0%
! CATCGAAGATAATTTGGGTT+GGG + chr2.3:8228480-8228499 MS.gene028748:CDS 35.0%
! GTTGTTTCCACAATTAGTGT+TGG + chr2.3:8229582-8229601 MS.gene028748:CDS 35.0%
! TAACTATAGGTATGATAGCG+GGG + chr2.3:8227623-8227642 MS.gene028748:intron 35.0%
! TAGTACCTTAATCCTTTTCC+CGG - chr2.3:8228739-8228758 None:intergenic 35.0%
! TTCACTTTCTATGTTGCTTC+AGG + chr2.3:8228433-8228452 MS.gene028748:CDS 35.0%
!! AACCAGCTGGGATTTTAAAA+TGG - chr2.3:8229339-8229358 None:intergenic 35.0%
!! ATCGTTGCTGATTCTTTCTT+TGG + chr2.3:8227784-8227803 MS.gene028748:CDS 35.0%
!! CAATTAGTGTTGGTTGGTAT+TGG + chr2.3:8229592-8229611 MS.gene028748:CDS 35.0%
!! TTAGTGATGGATCGTTCATT+AGG + chr2.3:8229310-8229329 MS.gene028748:CDS 35.0%
!! TTTCCACAATTAGTGTTGGT+TGG + chr2.3:8229586-8229605 MS.gene028748:CDS 35.0%
!! TTTTCCTCACTTTTCTTGAC+AGG + chr2.3:8229392-8229411 MS.gene028748:CDS 35.0%
!!! ACCAGCTGGGATTTTAAAAT+GGG - chr2.3:8229338-8229357 None:intergenic 35.0%
!!! GGTTTGGTTGTTTTTGTGTT+GGG + chr2.3:8228541-8228560 MS.gene028748:CDS 35.0%
!!! GTTGGGTTATCGATTTTATC+GGG + chr2.3:8228558-8228577 MS.gene028748:CDS 35.0%
!!! TGGTTTGGTTGTTTTTGTGT+TGG + chr2.3:8228540-8228559 MS.gene028748:CDS 35.0%
AAGAGAGAGACCAATGAAGA+AGG - chr2.3:8227487-8227506 None:intergenic 40.0%
ACGGTTGAAAAACCTGCATT+TGG - chr2.3:8229104-8229123 None:intergenic 40.0%
ACTTGCAAGCAATGGAATAG+TGG + chr2.3:8227651-8227670 MS.gene028748:CDS 40.0%
AGAGAGAGACCAATGAAGAA+GGG - chr2.3:8227486-8227505 None:intergenic 40.0%
CAATACCGGGAAAAGGATTA+AGG + chr2.3:8228731-8228750 MS.gene028748:CDS 40.0%
CACCAGTATATTTCCAGTAG+TGG + chr2.3:8227756-8227775 MS.gene028748:CDS 40.0%
CAGCGGTTTCAATGCTTATA+GGG + chr2.3:8229692-8229711 MS.gene028748:CDS 40.0%
CATCAGTTATTAGAGCTGCA+CGG - chr2.3:8229123-8229142 None:intergenic 40.0%
CATGTCCTACTCCTATTTGT+TGG - chr2.3:8229468-8229487 None:intergenic 40.0%
CTTGCAAGCAATGGAATAGT+GGG + chr2.3:8227652-8227671 MS.gene028748:CDS 40.0%
GAAAGAATCAGCAACGATTG+CGG - chr2.3:8227782-8227801 None:intergenic 40.0%
GATGTGCTCTTTAGTGATTG+TGG - chr2.3:8229667-8229686 None:intergenic 40.0%
GATTACTACGGTAGTTCTTC+TGG + chr2.3:8228676-8228695 MS.gene028748:CDS 40.0%
GCTGATCTATATCCAAATGC+AGG + chr2.3:8229089-8229108 MS.gene028748:intron 40.0%
GTGTATTGGATTCTTGTTGG+TGG - chr2.3:8228286-8228305 None:intergenic 40.0%
TACTGGATGTTAGTCGTACT+CGG + chr2.3:8229811-8229830 MS.gene028748:CDS 40.0%
TCCAGAGTAGAGGATTACTA+CGG + chr2.3:8228664-8228683 MS.gene028748:CDS 40.0%
TTCCCGTGATTTATGTCATC+AGG - chr2.3:8229778-8229797 None:intergenic 40.0%
TTTAGGGGTCCAACATTGTT+AGG + chr2.3:8228879-8228898 MS.gene028748:intron 40.0%
! CTTAATCCTTTTCCCGGTAT+TGG - chr2.3:8228733-8228752 None:intergenic 40.0%
! GGAGAAGCTTTTCATTTTCC+AGG + chr2.3:8229613-8229632 MS.gene028748:CDS 40.0%
! TTGAGTAATGGAAGAAAGCG+CGG + chr2.3:8227444-8227463 MS.gene028748:CDS 40.0%
!! GATAATTTGGGTTGGGCTAT+AGG + chr2.3:8228487-8228506 MS.gene028748:CDS 40.0%
!! GGAAGTGCGATTTTGGATTT+GGG + chr2.3:8228595-8228614 MS.gene028748:CDS 40.0%
!!! TCCCATTTTAAAATCCCAGC+TGG + chr2.3:8229334-8229353 MS.gene028748:CDS 40.0%
AAATAGGACATTTGGCACGC+TGG - chr2.3:8228095-8228114 None:intergenic 45.0%
AATAGGACATTTGGCACGCT+GGG - chr2.3:8228094-8228113 None:intergenic 45.0%
ACCGTAGTAATCCTCTACTC+TGG - chr2.3:8228668-8228687 None:intergenic 45.0%
ACTGGATGTTAGTCGTACTC+GGG + chr2.3:8229812-8229831 MS.gene028748:CDS 45.0%
ACTTTCACCTTTGCACTTGC+AGG + chr2.3:8228177-8228196 MS.gene028748:intron 45.0%
ATGGATGAACCATTGCTGCA+TGG - chr2.3:8228253-8228272 None:intergenic 45.0%
CCTAAGCTGCTCCAACAAAT+AGG + chr2.3:8229454-8229473 MS.gene028748:CDS 45.0%
CTGCTCCAACAAATAGGAGT+AGG + chr2.3:8229460-8229479 MS.gene028748:CDS 45.0%
CTTTCACCTTTGCACTTGCA+GGG + chr2.3:8228178-8228197 MS.gene028748:intron 45.0%
GAGAGAGACCAATGAAGAAG+GGG - chr2.3:8227485-8227504 None:intergenic 45.0%
GATGCAGATCTACACTCAGA+TGG + chr2.3:8229139-8229158 MS.gene028748:CDS 45.0%
GCAGATGAGAGCTTGAGTAA+TGG + chr2.3:8227432-8227451 MS.gene028748:CDS 45.0%
GCAGCGGTTTCAATGCTTAT+AGG + chr2.3:8229691-8229710 MS.gene028748:CDS 45.0%
GCATCCTGTCAAGAAAAGTG+AGG - chr2.3:8229399-8229418 None:intergenic 45.0%
GGAAGATCGACAATGTGTAC+TGG + chr2.3:8229794-8229813 MS.gene028748:CDS 45.0%
GGACATGTGCTGAATGTTCT+TGG + chr2.3:8229481-8229500 MS.gene028748:CDS 45.0%
GGCCACTACTGGAAATATAC+TGG - chr2.3:8227761-8227780 None:intergenic 45.0%
TAAGCTCTCTTCCAGAGTAG+AGG + chr2.3:8228654-8228673 MS.gene028748:CDS 45.0%
TGTGCTGAATGTTCTTGGCA+TGG + chr2.3:8229486-8229505 MS.gene028748:CDS 45.0%
TTGTTGCATCTGCTCGTAAG+TGG + chr2.3:8228626-8228645 MS.gene028748:CDS 45.0%
! AAAGAGCACATCAACTGCAG+CGG + chr2.3:8229675-8229694 MS.gene028748:CDS 45.0%
! AGGATTTGGTATCTGTGCTG+TGG + chr2.3:8228507-8228526 MS.gene028748:CDS 45.0%
! CCTATTTGTTGGAGCAGCTT+AGG - chr2.3:8229457-8229476 None:intergenic 45.0%
! GCTCTCATCTGCTTCTTTTG+AGG - chr2.3:8227424-8227443 None:intergenic 45.0%
!! AATCCTTTTCCCGGTATTGG+CGG - chr2.3:8228730-8228749 None:intergenic 45.0%
!! GGGAAGTGCGATTTTGGATT+TGG + chr2.3:8228594-8228613 MS.gene028748:CDS 45.0%
!! TGTTGGTGGTTTGCATGTGA+TGG - chr2.3:8228272-8228291 None:intergenic 45.0%
!! TTGGGTTGGGCTATAGGATT+TGG + chr2.3:8228493-8228512 MS.gene028748:CDS 45.0%
AAGCCTCATCCATGCAGCAA+TGG + chr2.3:8228241-8228260 MS.gene028748:CDS 50.0%
ACAGGATGCTATTGCCAGGT+TGG + chr2.3:8229410-8229429 MS.gene028748:CDS 50.0%
ATTGTGCCCTGCAAGTGCAA+AGG - chr2.3:8228187-8228206 None:intergenic 50.0%
CCAGTAACCTTATGCCAACC+TGG - chr2.3:8229427-8229446 None:intergenic 50.0%
CGTTTCACAGCAGCATCACT+AGG + chr2.3:8228355-8228374 MS.gene028748:CDS 50.0%
CTGGATGTTAGTCGTACTCG+GGG + chr2.3:8229813-8229832 MS.gene028748:CDS 50.0%
CTTGACAGGATGCTATTGCC+AGG + chr2.3:8229406-8229425 MS.gene028748:CDS 50.0%
GAACCATTGCTGCATGGATG+AGG - chr2.3:8228247-8228266 None:intergenic 50.0%
GCTGAATGTTCTTGGCATGG+TGG + chr2.3:8229489-8229508 MS.gene028748:CDS 50.0%
GCTGCATGGATGAGGCTTTA+AGG - chr2.3:8228239-8228258 None:intergenic 50.0%
GCTTCAGGAGCTGCATTAAC+CGG + chr2.3:8228448-8228467 MS.gene028748:CDS 50.0%
TATCGGGCTGAAAAGCCACA+GGG + chr2.3:8228574-8228593 MS.gene028748:CDS 50.0%
TCCAAAATCGCACTTCCCTG+TGG - chr2.3:8228592-8228611 None:intergenic 50.0%
TGGATGTTAGTCGTACTCGG+GGG + chr2.3:8229814-8229833 MS.gene028748:CDS 50.0%
TTATCGGGCTGAAAAGCCAC+AGG + chr2.3:8228573-8228592 MS.gene028748:CDS 50.0%
TTGCATCTGCTCGTAAGTGG+AGG + chr2.3:8228629-8228648 MS.gene028748:CDS 50.0%
! AGTAATGGAAGAAAGCGCGG+CGG + chr2.3:8227447-8227466 MS.gene028748:CDS 50.0%
! CCAGGTTGGCATAAGGTTAC+TGG + chr2.3:8229424-8229443 MS.gene028748:CDS 50.0%
! GCTATTGCCAGGTTGGCATA+AGG + chr2.3:8229417-8229436 MS.gene028748:CDS 50.0%
! GGGATGTCACTTGCAAGCAA+TGG + chr2.3:8227643-8227662 MS.gene028748:CDS 50.0%
! TGTGGCTGCCTTTGTTGGTT+TGG + chr2.3:8228525-8228544 MS.gene028748:CDS 50.0%
TGTGCTGTGGCTGCCTTTGT+TGG + chr2.3:8228520-8228539 MS.gene028748:CDS 55.0%
!! GCCACAGGGAAGTGCGATTT+TGG + chr2.3:8228588-8228607 MS.gene028748:CDS 55.0%
AGTGCTGCCTCCGCCAATAC+CGG + chr2.3:8228717-8228736 MS.gene028748:CDS 60.0%
CCTCCGCCAATACCGGGAAA+AGG + chr2.3:8228724-8228743 MS.gene028748:CDS 60.0%
GCAACGATTGCGGCCACTAC+TGG - chr2.3:8227772-8227791 None:intergenic 60.0%
! ATGGAAGAAAGCGCGGCGGT+TGG + chr2.3:8227451-8227470 MS.gene028748:CDS 60.0%
!!! CCTTTTCCCGGTATTGGCGG+AGG - chr2.3:8228727-8228746 None:intergenic 60.0%
GTGCTGCCTCCGCCAATACC+GGG + chr2.3:8228718-8228737 MS.gene028748:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 8227399 8229894 8227399 ID=MS.gene028748
chr2.3 mRNA 8227399 8229894 8227399 ID=MS.gene028748.t1;Parent=MS.gene028748
chr2.3 exon 8227399 8227495 8227399 ID=MS.gene028748.t1.exon1;Parent=MS.gene028748.t1
chr2.3 CDS 8227399 8227495 8227399 ID=cds.MS.gene028748.t1;Parent=MS.gene028748.t1
chr2.3 exon 8227632 8227849 8227632 ID=MS.gene028748.t1.exon2;Parent=MS.gene028748.t1
chr2.3 CDS 8227632 8227849 8227632 ID=cds.MS.gene028748.t1;Parent=MS.gene028748.t1
chr2.3 exon 8228199 8228752 8228199 ID=MS.gene028748.t1.exon3;Parent=MS.gene028748.t1
chr2.3 CDS 8228199 8228752 8228199 ID=cds.MS.gene028748.t1;Parent=MS.gene028748.t1
chr2.3 exon 8229111 8229894 8229111 ID=MS.gene028748.t1.exon4;Parent=MS.gene028748.t1
chr2.3 CDS 8229111 8229894 8229111 ID=cds.MS.gene028748.t1;Parent=MS.gene028748.t1
Gene Sequence

>MS.gene028748

ATGGATGGTCAGGTAGCAGCATCCTCAAAAGAAGCAGATGAGAGCTTGAGTAATGGAAGAAAGCGCGGCGGTTGGATAAGTTTCCCCTTCTTCATTGGTCTCTCTCTTTTTCCCTTTCATTTTTTAACTCATCAACATTATTAATTCACTCACTTTTACACATCAAAATCATTTTGTTTGAGTCTTATTAGATAAATGTTTATATTTTATTTTATACTGTTGATTAACTATAGGTATGATAGCGGGGATGTCACTTGCAAGCAATGGAATAGTGGGAAACTTGATTGTATATCTTACAAGAGAATTCAATATAAAGAGCATCACTGCAACTCAAATTTATAATGTTGTCGTTGGAAGCACCAGTATATTTCCAGTAGTGGCCGCAATCGTTGCTGATTCTTTCTTTGGTTCTTTCTCTGTTGCCTTTGCTTCTTCTTGTATTTTATTGCTGGTAAACTTTAACAATCAATCCAAATTATGTTGCATATATGCTAGCATTTTGTTTTGTCACGATAGACTAATAGCTAGTGTGACTAAGATAAATATTTACATCTAATATGGTTAGTCTATTCCATCATCAGTCATCACATATTTCCTCTATTACGTTCTTTTTTTAGAAGTGGTTTTTGAGTAAGTCATTAGAACGTTTGTTTACTTTTTTCAAGTTGGATATTGCATGTTCGAATTTGTGTCCCAGCGTGCCAAATGTCCTATTTTACAATATGATGTGCATTTTCATAACCAAACATACACTTATTTGTGGTAATATCTCTTTTAAACTTTCACCTTTGCACTTGCAGGGCACAATTCTATTATTCTTGACGGCAACTATCAATTCCTTAAAGCCTCATCCATGCAGCAATGGTTCATCCATCACATGCAAACCACCAACAAGAATCCAATACACAGTTTTATACATAAGCATATTATTAATATCAATTGGATTTGGTGGTTCACGTTTCACAGCAGCATCACTAGGTGCAAATCAGTTTGACAAACCAGAACATCAAGGAATATTTTTCAACTGGTACTTCTTCACTTTCTATGTTGCTTCAGGAGCTGCATTAACCGGAATTATTTACATCGAAGATAATTTGGGTTGGGCTATAGGATTTGGTATCTGTGCTGTGGCTGCCTTTGTTGGTTTGGTTGTTTTTGTGTTGGGTTATCGATTTTATCGGGCTGAAAAGCCACAGGGAAGTGCGATTTTGGATTTGGGACGTGTTTTTGTTGCATCTGCTCGTAAGTGGAGGTGTAAGCTCTCTTCCAGAGTAGAGGATTACTACGGTAGTTCTTCTGGTCATGATGTTTTGATTCAAGTGCTGCCTCCGCCAATACCGGGAAAAGGATTAAGGTACTAAGTATTAACCACACTTATTCTCATAGTTCAAATGTAGGGGAACGTAGTAACCTTGAATGCAGATAATCTTATTCTCATATAAAAACAACTTATAGCTTATATACAAATGATAGCTGACAGTTTAGGGGTCCAACATTGTTAGGATGTCTATTCTGTTATGTATTGCTTCTGCACTCTCGTTTATGCTTAAAATGAAACTTATAGCTTATATAAAAACAATTTAATTTGATTTTATCTTATGTTATAATTATAACAATAACTTATAAATAAACGTCTCATTAAGCTATTTATCCAAAGAATATGCTTGTAAAGTTTTGCTATATATCATGTGCTGATCTATATCCAAATGCAGGTTTTTCAACCGTGCAGCTCTAATAACTGATGCAGATCTACACTCAGATGGCACAATTAAGAAATCATCATGGAAACTATGCACAATTCAACAAGTGGAAGATTTCAAAAAAATAATTGGAATATTACCACTATGGACTTCAAGTCTATTCCTATCAATTCCAATAGTAATGCAAATGGACTTCATTATACTTCAAGCTTTAGTGATGGATCGTTCATTAGGATCCCATTTTAAAATCCCAGCTGGTTCTATTTCTGTTATAGTCTTAATATCAGCACCCATTTTCCTCACTTTTCTTGACAGGATGCTATTGCCAGGTTGGCATAAGGTTACTGGAAAATCGCCTAAGCTGCTCCAACAAATAGGAGTAGGACATGTGCTGAATGTTCTTGGCATGGTGGTCTCTGCTCTTGTTGAATCGAAGAGACTCAAATTAGCACGTGTGCATGATGTTTCAATGTCTGTGTTATGGTTGTTTCCACAATTAGTGTTGGTTGGTATTGGAGAAGCTTTTCATTTTCCAGGACAAGTTACATTCTATTATCAACAATTTCCACAATCACTAAAGAGCACATCAACTGCAGCGGTTTCAATGCTTATAGGGATTGCTTTTTACTTGAGCAATGCTTTAATTGACCAAGTTCATAGGAGTACTAATTGGCTACCTGATGACATAAATCACGGGAAGATCGACAATGTGTACTGGATGTTAGTCGTACTCGGGGGTATCAACTTTGTGTATTACTTATTTTGTTCTTATGCTTACAAGTATGAAAATGTTTAA
Protein sequence

>MS.gene028748.t1

MDGQVAASSKEADESLSNGRKRGGWISFPFFIGMIAGMSLASNGIVGNLIVYLTREFNIKSITATQIYNVVVGSTSIFPVVAAIVADSFFGSFSVAFASSCILLLGTILLFLTATINSLKPHPCSNGSSITCKPPTRIQYTVLYISILLISIGFGGSRFTAASLGANQFDKPEHQGIFFNWYFFTFYVASGAALTGIIYIEDNLGWAIGFGICAVAAFVGLVVFVLGYRFYRAEKPQGSAILDLGRVFVASARKWRCKLSSRVEDYYGSSSGHDVLIQVLPPPIPGKGLRFFNRAALITDADLHSDGTIKKSSWKLCTIQQVEDFKKIIGILPLWTSSLFLSIPIVMQMDFIILQALVMDRSLGSHFKIPAGSISVIVLISAPIFLTFLDRMLLPGWHKVTGKSPKLLQQIGVGHVLNVLGMVVSALVESKRLKLARVHDVSMSVLWLFPQLVLVGIGEAFHFPGQVTFYYQQFPQSLKSTSTAAVSMLIGIAFYLSNALIDQVHRSTNWLPDDINHGKIDNVYWMLVVLGGINFVYYLFCSYAYKYENV