AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31325

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31325.t1 MTR_2g093640 92.364 550 41 1 1 550 1 549 0.0 978
MS.gene31325.t1 MTR_2g093160 83.818 550 88 1 1 550 1 549 0.0 926
MS.gene31325.t1 MTR_2g093180 80.944 551 103 2 1 550 1 550 0.0 866
MS.gene31325.t1 MTR_4g107530 34.655 580 344 10 2 550 8 583 4.99e-111 343
MS.gene31325.t1 MTR_4g107580 34.370 579 340 11 1 548 4 573 5.61e-106 330
MS.gene31325.t1 MTR_5g038060 34.458 563 342 8 9 548 19 577 1.39e-105 329
MS.gene31325.t1 MTR_4g107510 34.812 586 327 12 8 550 13 586 1.79e-105 329
MS.gene31325.t1 MTR_8g469480 34.946 558 320 12 24 550 41 586 4.40e-102 320
MS.gene31325.t1 MTR_2g101650 35.866 566 324 14 13 550 14 568 7.60e-99 311
MS.gene31325.t1 MTR_2g101590 34.912 570 328 14 5 545 10 565 1.54e-96 307
MS.gene31325.t1 MTR_4g011280 34.281 563 333 15 9 545 18 569 2.87e-96 305
MS.gene31325.t1 MTR_5g055000 33.092 553 341 10 20 550 17 562 3.27e-96 304
MS.gene31325.t1 MTR_3g112460 32.855 557 348 9 1 542 1 546 2.23e-95 302
MS.gene31325.t1 MTR_2g101610 34.622 569 329 14 6 545 12 566 2.82e-95 303
MS.gene31325.t1 MTR_4g107610 32.299 548 345 8 17 543 27 569 3.66e-95 302
MS.gene31325.t1 MTR_7g088825 32.021 559 348 11 14 548 15 565 7.02e-95 301
MS.gene31325.t1 MTR_1g026740 35.159 566 329 15 5 545 1 553 2.45e-94 299
MS.gene31325.t1 MTR_2g101640 34.380 573 337 13 1 545 1 562 5.50e-94 300
MS.gene31325.t1 MTR_3g112470 33.034 557 338 10 2 542 9 546 1.93e-93 296
MS.gene31325.t1 MTR_3g462710 32.028 562 338 12 17 550 20 565 3.81e-93 296
MS.gene31325.t1 MTR_1g057460 34.588 558 326 13 6 545 28 564 1.58e-92 295
MS.gene31325.t1 MTR_7g067010 31.514 568 356 9 2 550 10 563 1.51e-91 292
MS.gene31325.t1 MTR_8g469470 32.671 554 347 9 20 549 31 582 3.22e-91 291
MS.gene31325.t1 MTR_5g038190 30.797 552 353 11 20 548 23 568 7.60e-90 288
MS.gene31325.t1 MTR_7g088790 31.922 567 358 10 4 548 12 572 4.01e-89 286
MS.gene31325.t1 MTR_2g101560 33.627 568 336 12 6 545 12 566 6.04e-89 287
MS.gene31325.t1 MTR_5g012270 32.252 555 340 11 21 549 29 573 1.13e-88 285
MS.gene31325.t1 MTR_1g026750 35.231 562 325 14 10 545 6 554 1.46e-88 284
MS.gene31325.t1 MTR_8g103233 33.091 550 332 12 20 545 16 553 2.71e-88 283
MS.gene31325.t1 MTR_7g088820 30.880 557 359 9 13 548 17 568 3.52e-88 284
MS.gene31325.t1 MTR_3g069500 32.228 543 340 9 21 550 35 562 1.04e-87 282
MS.gene31325.t1 MTR_7g098090 30.450 555 348 10 21 548 33 576 5.66e-86 278
MS.gene31325.t1 MTR_7g098160 29.818 550 353 9 21 548 33 571 6.07e-86 278
MS.gene31325.t1 MTR_4g015080 29.696 559 353 8 17 550 30 573 2.88e-85 276
MS.gene31325.t1 MTR_4g019580 31.835 556 344 10 18 550 28 571 5.61e-85 275
MS.gene31325.t1 MTR_7g098200 30.419 549 349 9 23 549 31 568 7.92e-85 275
MS.gene31325.t1 MTR_7g098040 28.909 550 358 10 21 548 34 572 1.20e-84 275
MS.gene31325.t1 MTR_7g065080 30.926 540 349 8 23 550 33 560 1.60e-84 274
MS.gene31325.t1 MTR_7g098200 30.055 549 341 10 23 549 31 558 4.07e-84 273
MS.gene31325.t1 MTR_3g112450 31.524 571 347 12 1 546 1 552 5.23e-84 272
MS.gene31325.t1 MTR_3g014790 31.362 558 326 9 22 549 18 548 7.12e-84 271
MS.gene31325.t1 MTR_1g009200 32.971 552 329 12 21 545 27 564 9.65e-84 272
MS.gene31325.t1 MTR_4g101380 31.153 581 353 12 2 549 8 574 9.82e-84 272
MS.gene31325.t1 MTR_1g057460 33.852 514 302 12 49 545 1 493 9.86e-84 270
MS.gene31325.t1 MTR_4g114340 32.364 550 340 11 17 546 20 557 1.08e-83 271
MS.gene31325.t1 MTR_5g012290 32.270 564 345 13 10 549 21 571 1.11e-83 272
MS.gene31325.t1 MTR_7g098180 30.576 556 346 12 21 550 31 572 1.12e-83 272
MS.gene31325.t1 MTR_3g069440 31.273 550 346 8 21 550 33 570 1.97e-83 271
MS.gene31325.t1 MTR_7g098220 31.250 576 348 12 2 550 16 570 2.80e-83 271
MS.gene31325.t1 MTR_1g026770 32.920 565 340 14 5 545 1 550 6.32e-83 269
MS.gene31325.t1 MTR_7g064780 31.810 569 356 11 8 549 6 569 1.44e-81 266
MS.gene31325.t1 MTR_1g075440 29.739 575 354 10 2 548 14 566 1.80e-81 266
MS.gene31325.t1 MTR_1g042310 29.877 569 361 11 2 548 12 564 2.41e-81 266
MS.gene31325.t1 MTR_7g098230 30.075 532 339 9 40 549 45 565 3.24e-81 265
MS.gene31325.t1 MTR_8g038880 31.119 572 343 11 7 550 4 552 8.40e-81 264
MS.gene31325.t1 MTR_7g098220 31.599 538 325 11 40 550 5 526 1.19e-80 263
MS.gene31325.t1 MTR_7g005930 29.137 556 371 8 6 546 20 567 3.15e-80 263
MS.gene31325.t1 MTR_4g033325 34.291 557 305 15 21 550 31 553 5.34e-80 261
MS.gene31325.t1 MTR_3g072300 30.420 572 355 12 2 549 10 562 1.08e-79 261
MS.gene31325.t1 MTR_1g042310 30.132 531 338 10 40 548 1 520 2.72e-79 259
MS.gene31325.t1 MTR_1g042310 30.132 531 338 10 40 548 8 527 3.95e-79 259
MS.gene31325.t1 MTR_0236s0050 32.749 513 309 11 49 545 1 493 1.19e-78 256
MS.gene31325.t1 MTR_7g065080 31.405 484 308 8 79 550 2 473 2.14e-78 255
MS.gene31325.t1 MTR_3g462710 32.836 469 271 12 109 549 3 455 2.32e-78 254
MS.gene31325.t1 MTR_5g014150 31.239 557 349 11 18 546 36 586 8.36e-78 257
MS.gene31325.t1 MTR_4g098800 31.157 536 338 10 39 549 54 583 1.14e-77 256
MS.gene31325.t1 MTR_7g088820 30.315 508 328 9 62 548 4 506 2.53e-77 253
MS.gene31325.t1 MTR_7g064800 31.376 545 355 6 15 540 20 564 3.14e-77 255
MS.gene31325.t1 MTR_4g098800 31.157 536 338 10 39 549 119 648 5.21e-77 256
MS.gene31325.t1 MTR_0294s0020 32.246 552 319 11 21 549 31 550 8.51e-77 253
MS.gene31325.t1 MTR_4g033315 31.643 572 333 14 1 549 16 552 3.11e-75 249
MS.gene31325.t1 MTR_4g033310 31.643 572 333 14 1 549 16 552 3.11e-75 249
MS.gene31325.t1 MTR_7g013270 29.358 545 360 8 19 546 88 624 4.38e-75 251
MS.gene31325.t1 MTR_1g116930 29.872 549 362 10 17 550 19 559 7.49e-75 248
MS.gene31325.t1 MTR_1g041875 28.489 523 350 8 21 529 29 541 1.36e-74 248
MS.gene31325.t1 MTR_4g033395 33.075 517 289 12 1 497 17 496 3.94e-74 244
MS.gene31325.t1 MTR_3g069420 30.991 555 337 10 23 550 36 571 4.61e-74 246
MS.gene31325.t1 MTR_1g041835 28.520 554 354 10 21 548 27 564 1.89e-73 244
MS.gene31325.t1 MTR_1g041815 28.970 573 360 11 2 548 13 564 3.43e-73 244
MS.gene31325.t1 MTR_4g033330 33.021 533 304 12 21 530 31 533 5.26e-73 242
MS.gene31325.t1 MTR_4g033375 32.558 516 293 11 1 497 17 496 1.83e-72 240
MS.gene31325.t1 MTR_7g098200 30.670 463 288 9 109 549 3 454 2.53e-71 236
MS.gene31325.t1 MTR_5g085850 29.412 544 359 11 17 548 26 556 5.17e-71 238
MS.gene31325.t1 MTR_7g010820 31.169 539 329 9 39 547 44 570 5.17e-71 238
MS.gene31325.t1 MTR_1g041885 27.470 506 343 8 40 531 1 496 8.55e-71 236
MS.gene31325.t1 MTR_2g005130 28.624 545 359 7 17 548 36 563 1.13e-69 235
MS.gene31325.t1 MTR_7g088820 30.519 462 295 9 108 548 2 458 1.28e-69 232
MS.gene31325.t1 MTR_3g005010 28.804 552 331 12 23 550 32 545 8.19e-69 231
MS.gene31325.t1 MTR_2g017750 29.314 539 350 11 39 548 52 588 1.69e-68 232
MS.gene31325.t1 MTR_7g098150 30.465 430 279 6 21 441 33 451 2.47e-68 229
MS.gene31325.t1 MTR_4g033325 34.389 442 241 13 136 550 10 429 1.57e-67 225
MS.gene31325.t1 MTR_4g033325 33.710 442 244 12 136 550 36 455 3.92e-67 224
MS.gene31325.t1 MTR_7g111260 28.233 549 356 14 20 549 18 547 4.40e-66 225
MS.gene31325.t1 MTR_5g038380 27.482 564 356 13 21 548 28 574 5.26e-66 225
MS.gene31325.t1 MTR_3g100980 27.240 558 359 13 20 550 15 552 7.02e-66 224
MS.gene31325.t1 MTR_4g033385 32.812 448 252 12 129 549 28 453 4.04e-65 219
MS.gene31325.t1 MTR_8g469310 27.143 560 362 16 21 548 26 571 1.65e-62 215
MS.gene31325.t1 MTR_1g085090 26.064 564 373 14 10 549 4 547 2.82e-62 215
MS.gene31325.t1 MTR_4g123537 28.345 568 369 14 2 549 22 571 7.49e-62 214
MS.gene31325.t1 MTR_2g093650 59.043 188 34 2 339 526 34 178 9.14e-62 202
MS.gene31325.t1 MTR_3g100980 25.641 585 362 13 20 550 15 580 6.70e-60 209
MS.gene31325.t1 MTR_4g107670 26.362 569 357 15 22 549 25 572 1.78e-59 207
MS.gene31325.t1 MTR_8g087780 26.389 576 389 10 1 548 6 574 2.84e-59 207
MS.gene31325.t1 MTR_8g091265 71.756 131 32 2 290 415 3 133 1.14e-57 189
MS.gene31325.t1 MTR_7g006540 26.596 564 378 12 14 548 56 612 2.28e-57 202
MS.gene31325.t1 MTR_8g087810 27.288 579 381 12 1 548 6 575 2.75e-57 201
MS.gene31325.t1 MTR_6g008690 25.688 545 345 11 34 546 19 535 2.13e-49 179
MS.gene31325.t1 MTR_4g024930 24.602 565 340 12 21 546 36 553 4.44e-49 179
MS.gene31325.t1 MTR_7g098210 26.538 520 287 10 44 549 5 443 6.78e-49 176
MS.gene31325.t1 MTR_7g051830 24.552 558 364 13 2 545 11 525 8.46e-49 177
MS.gene31325.t1 MTR_7g052600 24.687 559 362 15 2 545 11 525 5.87e-48 175
MS.gene31325.t1 MTR_7g051880 23.835 558 368 14 2 545 11 525 1.51e-46 171
MS.gene31325.t1 MTR_1g009170 29.250 400 212 11 149 528 9 357 1.11e-38 146
MS.gene31325.t1 MTR_4g033310 27.760 317 195 8 249 549 190 488 3.33e-31 127
MS.gene31325.t1 MTR_4g033310 34.536 194 105 5 1 189 16 192 1.35e-17 86.3
MS.gene31325.t1 MTR_8g091275 58.333 72 29 1 197 267 1 72 4.53e-24 96.7
MS.gene31325.t1 MTR_8g091480 59.420 69 22 2 221 289 27 89 6.69e-16 81.6
MS.gene31325.t1 MTR_3g065350 32.692 156 95 5 331 480 1 152 2.06e-12 65.9
MS.gene31325.t1 MTR_7g064160 31.915 94 63 1 15 107 23 116 7.64e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31325.t1 AT3G45660 49.551 557 261 4 1 550 1 544 0.0 558
MS.gene31325.t1 AT3G45660 49.551 557 261 4 1 550 1 544 0.0 558
MS.gene31325.t1 AT3G45660 49.551 557 261 4 1 550 22 565 0.0 558
MS.gene31325.t1 AT3G45650 49.462 558 261 6 1 550 1 545 0.0 554
MS.gene31325.t1 AT3G45680 49.270 548 273 3 3 550 2 544 0.0 541
MS.gene31325.t1 AT3G45710 47.905 549 276 3 2 550 8 546 0.0 540
MS.gene31325.t1 AT3G45650 47.260 584 261 7 1 550 1 571 0.0 539
MS.gene31325.t1 AT3G45700 46.875 544 278 4 7 550 2 534 1.84e-180 520
MS.gene31325.t1 AT3G45720 46.364 550 287 4 1 550 1 542 3.38e-177 511
MS.gene31325.t1 AT3G45690 45.185 540 250 6 11 550 8 501 1.93e-161 470
MS.gene31325.t1 AT1G69870 35.273 550 330 10 20 548 50 594 5.42e-110 341
MS.gene31325.t1 AT1G18880 35.428 573 321 10 11 550 12 568 1.89e-106 331
MS.gene31325.t1 AT3G47960 35.727 557 314 12 24 548 64 608 2.79e-103 324
MS.gene31325.t1 AT5G62680 33.798 574 328 12 13 548 33 592 3.22e-99 313
MS.gene31325.t1 AT1G27080 32.849 551 333 8 20 548 13 548 4.38e-98 309
MS.gene31325.t1 AT1G52190 33.099 568 343 13 8 545 6 566 2.32e-96 305
MS.gene31325.t1 AT3G16180 33.688 564 341 11 8 545 9 565 6.93e-96 304
MS.gene31325.t1 AT2G26690 33.574 554 336 11 10 546 18 556 6.09e-94 298
MS.gene31325.t1 AT3G54140 31.059 557 346 10 17 548 20 563 5.85e-93 295
MS.gene31325.t1 AT3G54140 31.059 557 346 10 17 548 20 563 5.85e-93 295
MS.gene31325.t1 AT1G62200 33.945 545 323 11 20 541 51 581 6.10e-93 296
MS.gene31325.t1 AT1G68570 33.391 575 353 11 1 550 1 570 1.23e-92 295
MS.gene31325.t1 AT1G27080 32.821 521 313 8 50 548 1 506 6.56e-92 291
MS.gene31325.t1 AT3G54450 29.663 563 345 8 15 550 6 544 1.12e-90 289
MS.gene31325.t1 AT2G26690 33.779 524 320 10 40 546 10 523 3.33e-90 288
MS.gene31325.t1 AT1G62200 33.099 568 326 12 20 541 51 610 3.64e-90 290
MS.gene31325.t1 AT1G62200 33.099 568 326 12 20 541 65 624 6.05e-90 290
MS.gene31325.t1 AT2G26690 33.846 520 317 10 44 546 2 511 1.05e-89 286
MS.gene31325.t1 AT1G68570 34.019 535 325 10 39 550 11 540 5.06e-88 283
MS.gene31325.t1 AT5G01180 30.741 553 356 9 17 550 21 565 1.65e-87 281
MS.gene31325.t1 AT5G01180 30.741 553 356 9 17 550 21 565 1.65e-87 281
MS.gene31325.t1 AT5G01180 30.741 553 356 9 17 550 21 565 1.65e-87 281
MS.gene31325.t1 AT1G69860 31.560 564 357 11 1 549 1 550 1.43e-83 271
MS.gene31325.t1 AT5G46040 31.115 556 327 12 9 529 11 545 4.26e-82 268
MS.gene31325.t1 AT5G28470 30.292 548 358 11 20 549 16 557 1.96e-81 265
MS.gene31325.t1 AT3G53960 30.319 564 366 11 5 550 21 575 5.75e-81 265
MS.gene31325.t1 AT5G28470 30.037 546 360 10 20 549 16 555 1.05e-80 263
MS.gene31325.t1 AT2G02020 30.380 553 315 10 20 550 41 545 2.23e-80 262
MS.gene31325.t1 AT2G37900 30.838 561 349 12 13 550 24 568 2.99e-79 260
MS.gene31325.t1 AT2G02040 30.287 558 346 12 20 549 40 582 4.78e-79 259
MS.gene31325.t1 AT2G40460 31.541 558 328 13 21 549 24 556 4.71e-78 257
MS.gene31325.t1 AT3G21670 30.826 545 345 13 21 548 29 558 4.11e-77 254
MS.gene31325.t1 AT2G37900 30.707 495 310 10 73 550 11 489 1.92e-73 242
MS.gene31325.t1 AT5G19640 28.598 535 360 9 22 546 60 582 1.95e-72 243
MS.gene31325.t1 AT1G32450 30.639 532 341 12 39 546 55 582 3.15e-72 242
MS.gene31325.t1 AT1G72125 30.961 562 326 14 18 550 30 558 3.76e-72 241
MS.gene31325.t1 AT5G46050 28.440 545 351 12 2 524 13 540 6.96e-72 240
MS.gene31325.t1 AT1G27040 28.772 570 369 11 1 547 1 556 1.09e-71 239
MS.gene31325.t1 AT1G27040 28.772 570 369 11 1 547 5 560 1.39e-71 239
MS.gene31325.t1 AT1G12110 30.159 567 352 11 10 549 21 570 7.34e-71 238
MS.gene31325.t1 AT1G27040 29.524 525 329 11 47 547 21 528 1.18e-70 236
MS.gene31325.t1 AT4G21680 28.962 549 360 10 23 547 30 572 2.46e-70 236
MS.gene31325.t1 AT1G33440 29.358 545 356 7 21 548 38 570 1.13e-69 235
MS.gene31325.t1 AT1G72140 28.315 558 329 11 23 549 36 553 4.55e-68 229
MS.gene31325.t1 AT1G69850 29.225 568 353 14 21 550 25 581 6.76e-68 230
MS.gene31325.t1 AT1G59740 28.991 545 358 9 17 542 37 571 1.30e-66 227
MS.gene31325.t1 AT1G22540 31.079 547 324 11 23 545 32 549 2.26e-66 225
MS.gene31325.t1 AT1G22550 29.603 554 340 10 23 550 32 561 5.72e-66 224
MS.gene31325.t1 AT1G22570 30.075 532 319 12 23 526 32 538 6.35e-66 224
MS.gene31325.t1 AT2G02020 29.295 553 300 12 20 550 41 524 1.43e-65 222
MS.gene31325.t1 AT1G72120 30.325 554 330 11 23 550 31 554 9.21e-64 218
MS.gene31325.t1 AT1G72130 29.336 542 330 9 23 547 27 532 1.35e-63 217
MS.gene31325.t1 AT1G22570 29.637 523 315 13 32 526 4 501 1.30e-62 214
MS.gene31325.t1 AT5G46050 29.018 448 266 10 108 524 2 428 8.49e-62 211
MS.gene31325.t1 AT3G25260 28.571 504 318 7 48 545 48 515 2.49e-60 208
MS.gene31325.t1 AT5G13400 26.018 565 382 12 14 550 57 613 2.18e-56 200
MS.gene31325.t1 AT5G14940 26.186 569 352 12 16 546 1 539 1.74e-55 196
MS.gene31325.t1 AT5G62730 25.654 573 326 12 27 549 5 527 1.85e-55 195
MS.gene31325.t1 AT5G62730 26.165 558 356 14 21 549 56 586 4.12e-55 196
MS.gene31325.t1 AT5G11570 27.140 549 309 13 13 545 3 476 5.04e-55 193
MS.gene31325.t1 AT1G72130 29.630 432 262 8 133 547 8 414 7.68e-54 188
MS.gene31325.t1 AT1G72130 29.630 432 262 8 133 547 8 414 7.68e-54 188
MS.gene31325.t1 AT3G25280 26.145 524 337 12 34 545 36 521 6.48e-48 174
MS.gene31325.t1 AT3G01350 24.866 559 346 11 34 546 19 549 1.42e-45 169
MS.gene31325.t1 AT2G38100 25.427 527 327 13 49 549 2 488 3.06e-34 136
MS.gene31325.t1 AT2G38100 25.427 527 327 13 49 549 18 504 4.85e-34 135
MS.gene31325.t1 AT2G38100 25.427 527 327 13 49 549 24 510 6.90e-34 135

Find 113 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTACATCGAAGATAATT+TGG 0.115376 2.4:+8370727 MS.gene31325:CDS
GCTGCATGGATGAGGCTTTA+AGG 0.172746 2.4:-8370489 None:intergenic
GCCACATGGAAGTGCGATTT+TGG 0.172832 2.4:+8370841 MS.gene31325:CDS
ATCGTTGCTGATTCTTTCTT+TGG 0.187254 2.4:+8370037 MS.gene31325:CDS
GCAGCGGTTTCAATGCTTAT+AGG 0.248050 2.4:+8371942 MS.gene31325:CDS
TTCACTTTCTATGTTGCTTC+AGG 0.254353 2.4:+8370686 MS.gene31325:CDS
CCAGGTTGGCATAAGGTTAC+TGG 0.278137 2.4:+8371675 MS.gene31325:CDS
GATAATTTGGGTTGGGCTAT+AGG 0.307130 2.4:+8370740 MS.gene31325:CDS
GTCAAGAAAAGTGAGGAAAA+TGG 0.307219 2.4:-8371640 None:intergenic
AAGTCCATTTGCATTACTAT+TGG 0.312103 2.4:-8371522 None:intergenic
TTGGGTTGGGCTATAGGATT+TGG 0.320970 2.4:+8370746 MS.gene31325:CDS
CAATTAGTGTTGGTTGGTAT+TGG 0.327167 2.4:+8371843 MS.gene31325:CDS
TGTGGCTGCCTTTGTTGGTT+TGG 0.335104 2.4:+8370778 MS.gene31325:CDS
GTCCTAATATCAATTGGATT+TGG 0.339605 2.4:+8370578 MS.gene31325:CDS
ATCTTCGATGTAAATAATTC+CGG 0.340345 2.4:-8370720 None:intergenic
ATAAGTTTCCCCTTCTTCAT+TGG 0.341967 2.4:+8369727 MS.gene31325:CDS
CACCAAATCCAATTGATATT+AGG 0.345339 2.4:-8370580 None:intergenic
CAGCGGTTTCAATGCTTATA+GGG 0.345560 2.4:+8371943 MS.gene31325:CDS
CCTAAGCTGCTCCAACAAAT+AGG 0.348313 2.4:+8371705 MS.gene31325:CDS
CTTTAATTGACCAGGTTCAT+AGG 0.360441 2.4:+8371988 MS.gene31325:CDS
GCATTACTATTGGAATTGAT+AGG 0.375721 2.4:-8371512 None:intergenic
ATTTACATCGAAGATAATTT+GGG 0.382217 2.4:+8370728 MS.gene31325:CDS
ATGTTTCAATGTCTGTGTTA+TGG 0.382577 2.4:+8371811 MS.gene31325:CDS
TTAGTGATGGATCGTTCATT+AGG 0.387822 2.4:+8371561 MS.gene31325:CDS
TGTGCTGTGGCTGCCTTTGT+TGG 0.388715 2.4:+8370773 MS.gene31325:CDS
GCAACGATTGCGGCCACTAC+TGG 0.405757 2.4:-8370022 None:intergenic
CATCGAAGATAATTTGGGTT+GGG 0.406002 2.4:+8370733 MS.gene31325:CDS
CACAATTCTATTATTCTTGA+CGG 0.406311 2.4:+8370454 MS.gene31325:CDS
TACTGGATGTTAGTCGTACT+CGG 0.406589 2.4:+8372062 MS.gene31325:CDS
TTCCCGTGATTTATGTCATC+AGG 0.412476 2.4:-8372026 None:intergenic
TAATTGGAATATTACCACTA+TGG 0.412509 2.4:+8371475 MS.gene31325:CDS
AGCATAGTCCTAATATCAAT+TGG 0.412840 2.4:+8370572 MS.gene31325:CDS
GCTTCAGGAGCTGCATTAAC+CGG 0.417423 2.4:+8370701 MS.gene31325:CDS
CATGTCCTACTCCTATTTGT+TGG 0.420471 2.4:-8371716 None:intergenic
CCTATTTGTTGGAGCAGCTT+AGG 0.424589 2.4:-8371705 None:intergenic
TGTTGGTGGTTTGCATGTGA+TGG 0.432040 2.4:-8370522 None:intergenic
AGTGCTGCCTCCGCCAATAC+CGG 0.445352 2.4:+8370970 MS.gene31325:CDS
CTAATATCAATTGGATTTGG+TGG 0.450715 2.4:+8370581 MS.gene31325:CDS
TCAAGAAAAGTGAGGAAAAT+GGG 0.451760 2.4:-8371639 None:intergenic
CAAATTTATAATGTTGTCGT+TGG 0.452697 2.4:+8369983 MS.gene31325:CDS
TAATAGAATGTAACTTGTCC+TGG 0.454723 2.4:-8371882 None:intergenic
ACTGTGTATTGGATTCTTGT+TGG 0.456215 2.4:-8370539 None:intergenic
AATTCCAATAGTAATGCAAA+TGG 0.460508 2.4:+8371518 MS.gene31325:CDS
ACTTGCAAGCAATGGAATAG+TGG 0.463177 2.4:+8369904 MS.gene31325:CDS
GGCCACTACTGGAAATATAC+TGG 0.468506 2.4:-8370011 None:intergenic
GGACATGTGCTGAATGTTCT+TGG 0.468739 2.4:+8371732 MS.gene31325:CDS
CTTGACAGGATGCTATTGCC+AGG 0.474278 2.4:+8371657 MS.gene31325:CDS
CCAGTAACCTTATGCCAACC+TGG 0.475202 2.4:-8371675 None:intergenic
TTGTTGCATCTGCTCGTAAG+TGG 0.481137 2.4:+8370879 MS.gene31325:CDS
TTTCCACAATTAGTGTTGGT+TGG 0.482453 2.4:+8371837 MS.gene31325:CDS
AGAGAGAGACCAATGAAGAA+GGG 0.482917 2.4:-8369736 None:intergenic
GCTATTGCCAGGTTGGCATA+AGG 0.485413 2.4:+8371668 MS.gene31325:CDS
AAGAGAGAGACCAATGAAGA+AGG 0.489487 2.4:-8369737 None:intergenic
ATGGAAGAAAGCGCGGCGGT+TGG 0.496987 2.4:+8369704 MS.gene31325:CDS
CCTCCGCCAATACCGGGAAA+AGG 0.506457 2.4:+8370977 MS.gene31325:CDS
GTGTATTGGATTCTTGTTGG+TGG 0.509836 2.4:-8370536 None:intergenic
GTGTGCAACAATGGATGGTC+AGG 0.509946 2.4:+8369642 None:intergenic
TTATGTATAGAACTGTGTAT+TGG 0.516541 2.4:-8370550 None:intergenic
ACATCGAAGATAATTTGGGT+TGG 0.520616 2.4:+8370732 MS.gene31325:CDS
CGTTTCACAGCAGCATCACT+AGG 0.522507 2.4:+8370608 MS.gene31325:CDS
GTGCTGCCTCCGCCAATACC+GGG 0.523813 2.4:+8370971 MS.gene31325:CDS
TATACTTCAAGCTTTAGTGA+TGG 0.526822 2.4:+8371548 MS.gene31325:CDS
GCAGATGAGAGCTTGAGTAA+TGG 0.528220 2.4:+8369685 MS.gene31325:CDS
ACAGGATGCTATTGCCAGGT+TGG 0.530502 2.4:+8371661 MS.gene31325:CDS
GTTGTTTCCACAATTAGTGT+TGG 0.534629 2.4:+8371833 MS.gene31325:CDS
ATACCAACCAACACTAATTG+TGG 0.537554 2.4:-8371840 None:intergenic
TTAACTATAGGTATGATAGC+GGG 0.538516 2.4:+8369875 MS.gene31325:intron
CTTGCAAGCAATGGAATAGT+GGG 0.541989 2.4:+8369905 MS.gene31325:CDS
GAGAGAGACCAATGAAGAAG+GGG 0.547308 2.4:-8369735 None:intergenic
GGAAGATCGACAATGTGTAC+TGG 0.551643 2.4:+8372045 MS.gene31325:CDS
CACCAGTATATTTCCAGTAG+TGG 0.555389 2.4:+8370009 MS.gene31325:CDS
ACTGGATGTTAGTCGTACTC+GGG 0.556684 2.4:+8372063 MS.gene31325:CDS
GAACCATTGCTGCATGGATG+AGG 0.561384 2.4:-8370497 None:intergenic
ATAACAGAAATAGAACCAGC+TGG 0.562257 2.4:-8371600 None:intergenic
GGGATGTCACTTGCAAGCAA+TGG 0.563554 2.4:+8369896 MS.gene31325:CDS
GAGCAATGCTTTAATTGACC+AGG 0.568016 2.4:+8371980 MS.gene31325:CDS
CTACCTGATGACATAAATCA+CGG 0.570213 2.4:+8372023 MS.gene31325:CDS
ATTAACTATAGGTATGATAG+CGG 0.570505 2.4:+8369874 MS.gene31325:intron
ACCGTAGTAATCCTCTACTC+TGG 0.578927 2.4:-8370918 None:intergenic
TAAAATACAAGAAGAAGCAA+AGG 0.580215 2.4:-8370074 None:intergenic
CTGGATGTTAGTCGTACTCG+GGG 0.588713 2.4:+8372064 MS.gene31325:CDS
CAATACCGGGAAAAGGATTG+AGG 0.592959 2.4:+8370984 MS.gene31325:CDS
CTGCTCCAACAAATAGGAGT+AGG 0.598863 2.4:+8371711 MS.gene31325:CDS
TGTGCTGAATGTTCTTGGCA+TGG 0.599136 2.4:+8371737 MS.gene31325:CDS
AAGCCTCATCCATGCAGCAA+TGG 0.601337 2.4:+8370494 MS.gene31325:CDS
AATCAGTACTCCTATGAACC+TGG 0.602544 2.4:-8371998 None:intergenic
GCTGAATGTTCTTGGCATGG+TGG 0.607060 2.4:+8371740 MS.gene31325:CDS
ACTATGCACAATTCAACAAG+TGG 0.607651 2.4:+8371437 MS.gene31325:CDS
GAAAGAATCAGCAACGATTG+CGG 0.609321 2.4:-8370032 None:intergenic
TAACTATAGGTATGATAGCG+GGG 0.610759 2.4:+8369876 MS.gene31325:intron
TGAATTGTGCATAGTCTCCA+TGG 0.612472 2.4:-8371429 None:intergenic
TAAGCTCTCTTCCAGAGTAG+AGG 0.612591 2.4:+8370907 MS.gene31325:CDS
TTTGACAAACCAGAACATCA+AGG 0.615786 2.4:+8370641 MS.gene31325:CDS
AATAGACTTGAAGTCCATAG+TGG 0.622304 2.4:-8371489 None:intergenic
AGGTTCATAGGAGTACTGAT+TGG 0.622425 2.4:+8372000 MS.gene31325:CDS
TAACAGAAATAGAACCAGCT+GGG 0.623287 2.4:-8371599 None:intergenic
AGGATTTGGTATCTGTGCTG+TGG 0.626074 2.4:+8370760 MS.gene31325:CDS
TACCTGATGACATAAATCAC+GGG 0.627724 2.4:+8372024 MS.gene31325:CDS
TCCAGAGTAGAGGATTACTA+CGG 0.636666 2.4:+8370917 MS.gene31325:CDS
TCCAAAATCGCACTTCCATG+TGG 0.636823 2.4:-8370842 None:intergenic
GATGTGCTCTTTAGTGATTG+TGG 0.636964 2.4:-8371915 None:intergenic
GATGCAGATCTACACTCAGA+TGG 0.639318 2.4:+8371390 MS.gene31325:CDS
ATGGATGAACCATTGCTGCA+TGG 0.645148 2.4:-8370503 None:intergenic
GTGAAGTGTGCAACAATGGA+TGG 0.650336 2.4:+8369637 None:intergenic
CATCACTTATTAGAGCTGCA+CGG 0.652379 2.4:-8371371 None:intergenic
TATCGGGCTGAAAAGCCACA+TGG 0.653365 2.4:+8370827 MS.gene31325:CDS
TTGCATCTGCTCGTAAGTGG+AGG 0.661442 2.4:+8370882 MS.gene31325:CDS
TTGAGTAATGGAAGAAAGCG+CGG 0.673268 2.4:+8369697 MS.gene31325:CDS
AAAGAGCACATCAACTGCAG+CGG 0.686439 2.4:+8371926 MS.gene31325:CDS
GCATCCTGTCAAGAAAAGTG+AGG 0.686991 2.4:-8371647 None:intergenic
GCACAATTAAGAAATCACCA+TGG 0.695213 2.4:+8371412 MS.gene31325:CDS
TGGATGTTAGTCGTACTCGG+GGG 0.758348 2.4:+8372065 MS.gene31325:CDS
AGTAATGGAAGAAAGCGCGG+CGG 0.807785 2.4:+8369700 MS.gene31325:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATATTTACATCTAATA+TGG + chr2.4:8370190-8370209 MS.gene31325:intron 10.0%
!! GAAGATTTCAAAAAAATAAT+TGG + chr2.4:8371459-8371478 MS.gene31325:CDS 15.0%
!! AGCATATATGCAATATAATT+TGG - chr2.4:8370125-8370144 None:intergenic 20.0%
!! ATATTGTAAAATAGGACATT+TGG - chr2.4:8370356-8370375 None:intergenic 20.0%
!! ATTTACATCGAAGATAATTT+GGG + chr2.4:8370728-8370747 MS.gene31325:CDS 20.0%
!! TATTTACATCGAAGATAATT+TGG + chr2.4:8370727-8370746 MS.gene31325:CDS 20.0%
!! TGATGAGTTAAAAAATGAAA+GGG - chr2.4:8369766-8369785 None:intergenic 20.0%
!! TTGATGAGTTAAAAAATGAA+AGG - chr2.4:8369767-8369786 None:intergenic 20.0%
!!! TTATACTGTTGATTAACTAT+AGG + chr2.4:8369863-8369882 MS.gene31325:intron 20.0%
!!! TTTGTTTACTTTTTTCAAGT+TGG + chr2.4:8370298-8370317 MS.gene31325:intron 20.0%
! AAAAATATTCCTTGATGTTC+TGG - chr2.4:8370653-8370672 None:intergenic 25.0%
! AACTTTACAAGCATATTCTT+TGG - chr2.4:8371304-8371323 None:intergenic 25.0%
! AATAAGATTATCTGCATTCA+AGG - chr2.4:8371069-8371088 None:intergenic 25.0%
! AATTCCAATAGTAATGCAAA+TGG + chr2.4:8371518-8371537 MS.gene31325:CDS 25.0%
! ATCTTCGATGTAAATAATTC+CGG - chr2.4:8370723-8370742 None:intergenic 25.0%
! ATTAACTATAGGTATGATAG+CGG + chr2.4:8369874-8369893 MS.gene31325:intron 25.0%
! ATTCTCATAGTTCAAATGTA+GGG + chr2.4:8371028-8371047 MS.gene31325:intron 25.0%
! CAAATTTATAATGTTGTCGT+TGG + chr2.4:8369983-8370002 MS.gene31325:CDS 25.0%
! CACAATTCTATTATTCTTGA+CGG + chr2.4:8370454-8370473 MS.gene31325:CDS 25.0%
! CTAAAAAAAGAACGTAATAG+AGG - chr2.4:8370249-8370268 None:intergenic 25.0%
! TAAAATACAAGAAGAAGCAA+AGG - chr2.4:8370077-8370096 None:intergenic 25.0%
! TAATTGGAATATTACCACTA+TGG + chr2.4:8371475-8371494 MS.gene31325:CDS 25.0%
! TATTCTCATAGTTCAAATGT+AGG + chr2.4:8371027-8371046 MS.gene31325:intron 25.0%
! TGCACATCATATTGTAAAAT+AGG - chr2.4:8370364-8370383 None:intergenic 25.0%
! TTATGTATAGAACTGTGTAT+TGG - chr2.4:8370553-8370572 None:intergenic 25.0%
!! TTCTTCTTGTATTTTATTGC+TGG + chr2.4:8370081-8370100 MS.gene31325:CDS 25.0%
!!! ATCAAGGAATATTTTTCAAC+TGG + chr2.4:8370657-8370676 MS.gene31325:CDS 25.0%
!!! TTACGTTCTTTTTTTAGAAG+TGG + chr2.4:8370252-8370271 MS.gene31325:intron 25.0%
AAAAACAACCAAACCAACAA+AGG - chr2.4:8370789-8370808 None:intergenic 30.0%
AAATATGTGATGACTGATGA+TGG - chr2.4:8370226-8370245 None:intergenic 30.0%
AAATGATAGCTGACAGTTTA+GGG + chr2.4:8371120-8371139 MS.gene31325:intron 30.0%
AAGTCCATTTGCATTACTAT+TGG - chr2.4:8371525-8371544 None:intergenic 30.0%
AATAGACATCCTAACAATGT+TGG - chr2.4:8371148-8371167 None:intergenic 30.0%
ACCAAACATACACTTATTTG+TGG + chr2.4:8370392-8370411 MS.gene31325:intron 30.0%
ACCACAAATAAGTGTATGTT+TGG - chr2.4:8370396-8370415 None:intergenic 30.0%
AGCATAGTCCTAATATCAAT+TGG + chr2.4:8370572-8370591 MS.gene31325:CDS 30.0%
ATGTTTCAATGTCTGTGTTA+TGG + chr2.4:8371811-8371830 MS.gene31325:CDS 30.0%
CACCAAATCCAATTGATATT+AGG - chr2.4:8370583-8370602 None:intergenic 30.0%
CTAATATCAATTGGATTTGG+TGG + chr2.4:8370581-8370600 MS.gene31325:CDS 30.0%
GCATTACTATTGGAATTGAT+AGG - chr2.4:8371515-8371534 None:intergenic 30.0%
GTCCTAATATCAATTGGATT+TGG + chr2.4:8370578-8370597 MS.gene31325:CDS 30.0%
TCAAGAAAAGTGAGGAAAAT+GGG - chr2.4:8371642-8371661 None:intergenic 30.0%
TTAACTATAGGTATGATAGC+GGG + chr2.4:8369875-8369894 MS.gene31325:intron 30.0%
TTCTCATAGTTCAAATGTAG+GGG + chr2.4:8371029-8371048 MS.gene31325:intron 30.0%
TTGAACTATGAGAATAAGTG+TGG - chr2.4:8371023-8371042 None:intergenic 30.0%
! TAATAGAATGTAACTTGTCC+TGG - chr2.4:8371885-8371904 None:intergenic 30.0%
! TATACTTCAAGCTTTAGTGA+TGG + chr2.4:8371548-8371567 MS.gene31325:CDS 30.0%
!!! TGTTGGGTTATCGATTTTAT+CGG + chr2.4:8370810-8370829 MS.gene31325:CDS 30.0%
AATAGACTTGAAGTCCATAG+TGG - chr2.4:8371492-8371511 None:intergenic 35.0%
AATGATAGCTGACAGTTTAG+GGG + chr2.4:8371121-8371140 MS.gene31325:intron 35.0%
ACATCGAAGATAATTTGGGT+TGG + chr2.4:8370732-8370751 MS.gene31325:CDS 35.0%
ACTATGCACAATTCAACAAG+TGG + chr2.4:8371437-8371456 MS.gene31325:CDS 35.0%
ACTGTGTATTGGATTCTTGT+TGG - chr2.4:8370542-8370561 None:intergenic 35.0%
ATAACAGAAATAGAACCAGC+TGG - chr2.4:8371603-8371622 None:intergenic 35.0%
ATAAGTTTCCCCTTCTTCAT+TGG + chr2.4:8369727-8369746 MS.gene31325:CDS 35.0%
ATACCAACCAACACTAATTG+TGG - chr2.4:8371843-8371862 None:intergenic 35.0%
CAAATGATAGCTGACAGTTT+AGG + chr2.4:8371119-8371138 MS.gene31325:intron 35.0%
CTACCTGATGACATAAATCA+CGG + chr2.4:8372023-8372042 MS.gene31325:CDS 35.0%
CTTTAATTGACCAGGTTCAT+AGG + chr2.4:8371988-8372007 MS.gene31325:CDS 35.0%
GCACAATTAAGAAATCACCA+TGG + chr2.4:8371412-8371431 MS.gene31325:CDS 35.0%
GTCAAGAAAAGTGAGGAAAA+TGG - chr2.4:8371643-8371662 None:intergenic 35.0%
TAACAGAAATAGAACCAGCT+GGG - chr2.4:8371602-8371621 None:intergenic 35.0%
TACCTGATGACATAAATCAC+GGG + chr2.4:8372024-8372043 MS.gene31325:CDS 35.0%
TTTGACAAACCAGAACATCA+AGG + chr2.4:8370641-8370660 MS.gene31325:CDS 35.0%
! CATCGAAGATAATTTGGGTT+GGG + chr2.4:8370733-8370752 MS.gene31325:CDS 35.0%
! GATTACTACGGTAGTTTTTC+TGG + chr2.4:8370929-8370948 MS.gene31325:CDS 35.0%
! GTTGTTTCCACAATTAGTGT+TGG + chr2.4:8371833-8371852 MS.gene31325:CDS 35.0%
! TAACTATAGGTATGATAGCG+GGG + chr2.4:8369876-8369895 MS.gene31325:intron 35.0%
! TTCACTTTCTATGTTGCTTC+AGG + chr2.4:8370686-8370705 MS.gene31325:CDS 35.0%
!! AACCAGCTGGGATTTTAAAA+TGG - chr2.4:8371590-8371609 None:intergenic 35.0%
!! ATCGTTGCTGATTCTTTCTT+TGG + chr2.4:8370037-8370056 MS.gene31325:CDS 35.0%
!! CAATTAGTGTTGGTTGGTAT+TGG + chr2.4:8371843-8371862 MS.gene31325:CDS 35.0%
!! TTAGTGATGGATCGTTCATT+AGG + chr2.4:8371561-8371580 MS.gene31325:CDS 35.0%
!! TTTCCACAATTAGTGTTGGT+TGG + chr2.4:8371837-8371856 MS.gene31325:CDS 35.0%
!! TTTTCCTCACTTTTCTTGAC+AGG + chr2.4:8371643-8371662 MS.gene31325:CDS 35.0%
!!! ACCAGCTGGGATTTTAAAAT+GGG - chr2.4:8371589-8371608 None:intergenic 35.0%
!!! GGTTTGGTTGTTTTTGTGTT+GGG + chr2.4:8370794-8370813 MS.gene31325:CDS 35.0%
!!! GTTGGGTTATCGATTTTATC+GGG + chr2.4:8370811-8370830 MS.gene31325:CDS 35.0%
!!! TGGTTTGGTTGTTTTTGTGT+TGG + chr2.4:8370793-8370812 MS.gene31325:CDS 35.0%
AAGAGAGAGACCAATGAAGA+AGG - chr2.4:8369740-8369759 None:intergenic 40.0%
AATCAGTACTCCTATGAACC+TGG - chr2.4:8372001-8372020 None:intergenic 40.0%
ACGGTTGAAAAACCTGCATT+TGG - chr2.4:8371355-8371374 None:intergenic 40.0%
ACTTGCAAGCAATGGAATAG+TGG + chr2.4:8369904-8369923 MS.gene31325:CDS 40.0%
AGAGAGAGACCAATGAAGAA+GGG - chr2.4:8369739-8369758 None:intergenic 40.0%
CACCAGTATATTTCCAGTAG+TGG + chr2.4:8370009-8370028 MS.gene31325:CDS 40.0%
CAGCGGTTTCAATGCTTATA+GGG + chr2.4:8371943-8371962 MS.gene31325:CDS 40.0%
CATCACTTATTAGAGCTGCA+CGG - chr2.4:8371374-8371393 None:intergenic 40.0%
CATGTCCTACTCCTATTTGT+TGG - chr2.4:8371719-8371738 None:intergenic 40.0%
CTTGCAAGCAATGGAATAGT+GGG + chr2.4:8369905-8369924 MS.gene31325:CDS 40.0%
GAAAGAATCAGCAACGATTG+CGG - chr2.4:8370035-8370054 None:intergenic 40.0%
GAGCAATGCTTTAATTGACC+AGG + chr2.4:8371980-8371999 MS.gene31325:CDS 40.0%
GATGTGCTCTTTAGTGATTG+TGG - chr2.4:8371918-8371937 None:intergenic 40.0%
GCTGATCTATATCCAAATGC+AGG + chr2.4:8371340-8371359 MS.gene31325:intron 40.0%
GTGTATTGGATTCTTGTTGG+TGG - chr2.4:8370539-8370558 None:intergenic 40.0%
TACTGGATGTTAGTCGTACT+CGG + chr2.4:8372062-8372081 MS.gene31325:CDS 40.0%
TCCAGAGTAGAGGATTACTA+CGG + chr2.4:8370917-8370936 MS.gene31325:CDS 40.0%
TGAATTGTGCATAGTCTCCA+TGG - chr2.4:8371432-8371451 None:intergenic 40.0%
TTCCCGTGATTTATGTCATC+AGG - chr2.4:8372029-8372048 None:intergenic 40.0%
TTTAGGGGTCCAACATTGTT+AGG + chr2.4:8371136-8371155 MS.gene31325:intron 40.0%
! GGAGAAGCTTTTCATTTTCC+AGG + chr2.4:8371864-8371883 MS.gene31325:CDS 40.0%
! TAGTACCTCAATCCTTTTCC+CGG - chr2.4:8370992-8371011 None:intergenic 40.0%
! TTGAGTAATGGAAGAAAGCG+CGG + chr2.4:8369697-8369716 MS.gene31325:CDS 40.0%
!! AGGTTCATAGGAGTACTGAT+TGG + chr2.4:8372000-8372019 MS.gene31325:CDS 40.0%
!! GATAATTTGGGTTGGGCTAT+AGG + chr2.4:8370740-8370759 MS.gene31325:CDS 40.0%
!! GGAAGTGCGATTTTGGATTT+GGG + chr2.4:8370848-8370867 MS.gene31325:CDS 40.0%
!! TGGAAGTGCGATTTTGGATT+TGG + chr2.4:8370847-8370866 MS.gene31325:CDS 40.0%
!!! TCCCATTTTAAAATCCCAGC+TGG + chr2.4:8371585-8371604 MS.gene31325:CDS 40.0%
AAATAGGACATTTGGCACGC+TGG - chr2.4:8370348-8370367 None:intergenic 45.0%
AATAGGACATTTGGCACGCT+GGG - chr2.4:8370347-8370366 None:intergenic 45.0%
ACCGTAGTAATCCTCTACTC+TGG - chr2.4:8370921-8370940 None:intergenic 45.0%
ACTGGATGTTAGTCGTACTC+GGG + chr2.4:8372063-8372082 MS.gene31325:CDS 45.0%
ACTTTCACCTTTGCACTTGC+AGG + chr2.4:8370430-8370449 MS.gene31325:intron 45.0%
ATGGATGAACCATTGCTGCA+TGG - chr2.4:8370506-8370525 None:intergenic 45.0%
CAATACCGGGAAAAGGATTG+AGG + chr2.4:8370984-8371003 MS.gene31325:CDS 45.0%
CCTAAGCTGCTCCAACAAAT+AGG + chr2.4:8371705-8371724 MS.gene31325:CDS 45.0%
CTGCTCCAACAAATAGGAGT+AGG + chr2.4:8371711-8371730 MS.gene31325:CDS 45.0%
CTTTCACCTTTGCACTTGCA+GGG + chr2.4:8370431-8370450 MS.gene31325:intron 45.0%
GAGAGAGACCAATGAAGAAG+GGG - chr2.4:8369738-8369757 None:intergenic 45.0%
GATGCAGATCTACACTCAGA+TGG + chr2.4:8371390-8371409 MS.gene31325:CDS 45.0%
GCAGATGAGAGCTTGAGTAA+TGG + chr2.4:8369685-8369704 MS.gene31325:CDS 45.0%
GCAGCGGTTTCAATGCTTAT+AGG + chr2.4:8371942-8371961 MS.gene31325:CDS 45.0%
GCATCCTGTCAAGAAAAGTG+AGG - chr2.4:8371650-8371669 None:intergenic 45.0%
GGAAGATCGACAATGTGTAC+TGG + chr2.4:8372045-8372064 MS.gene31325:CDS 45.0%
GGACATGTGCTGAATGTTCT+TGG + chr2.4:8371732-8371751 MS.gene31325:CDS 45.0%
GGCCACTACTGGAAATATAC+TGG - chr2.4:8370014-8370033 None:intergenic 45.0%
TAAGCTCTCTTCCAGAGTAG+AGG + chr2.4:8370907-8370926 MS.gene31325:CDS 45.0%
TCCAAAATCGCACTTCCATG+TGG - chr2.4:8370845-8370864 None:intergenic 45.0%
TGTGCTGAATGTTCTTGGCA+TGG + chr2.4:8371737-8371756 MS.gene31325:CDS 45.0%
TTGTTGCATCTGCTCGTAAG+TGG + chr2.4:8370879-8370898 MS.gene31325:CDS 45.0%
! AAAGAGCACATCAACTGCAG+CGG + chr2.4:8371926-8371945 MS.gene31325:CDS 45.0%
! AGGATTTGGTATCTGTGCTG+TGG + chr2.4:8370760-8370779 MS.gene31325:CDS 45.0%
! CCTATTTGTTGGAGCAGCTT+AGG - chr2.4:8371708-8371727 None:intergenic 45.0%
! CTCAATCCTTTTCCCGGTAT+TGG - chr2.4:8370986-8371005 None:intergenic 45.0%
! GCTCTCATCTGCTTCTTTTG+AGG - chr2.4:8369677-8369696 None:intergenic 45.0%
!! AATCCTTTTCCCGGTATTGG+CGG - chr2.4:8370983-8371002 None:intergenic 45.0%
!! TGTTGGTGGTTTGCATGTGA+TGG - chr2.4:8370525-8370544 None:intergenic 45.0%
!! TTGGGTTGGGCTATAGGATT+TGG + chr2.4:8370746-8370765 MS.gene31325:CDS 45.0%
AAGCCTCATCCATGCAGCAA+TGG + chr2.4:8370494-8370513 MS.gene31325:CDS 50.0%
ACAGGATGCTATTGCCAGGT+TGG + chr2.4:8371661-8371680 MS.gene31325:CDS 50.0%
ATTGTGCCCTGCAAGTGCAA+AGG - chr2.4:8370440-8370459 None:intergenic 50.0%
CCAGTAACCTTATGCCAACC+TGG - chr2.4:8371678-8371697 None:intergenic 50.0%
CGTTTCACAGCAGCATCACT+AGG + chr2.4:8370608-8370627 MS.gene31325:CDS 50.0%
CTGGATGTTAGTCGTACTCG+GGG + chr2.4:8372064-8372083 MS.gene31325:CDS 50.0%
CTTGACAGGATGCTATTGCC+AGG + chr2.4:8371657-8371676 MS.gene31325:CDS 50.0%
GAACCATTGCTGCATGGATG+AGG - chr2.4:8370500-8370519 None:intergenic 50.0%
GCTGAATGTTCTTGGCATGG+TGG + chr2.4:8371740-8371759 MS.gene31325:CDS 50.0%
GCTGCATGGATGAGGCTTTA+AGG - chr2.4:8370492-8370511 None:intergenic 50.0%
GCTTCAGGAGCTGCATTAAC+CGG + chr2.4:8370701-8370720 MS.gene31325:CDS 50.0%
TATCGGGCTGAAAAGCCACA+TGG + chr2.4:8370827-8370846 MS.gene31325:CDS 50.0%
TGGATGTTAGTCGTACTCGG+GGG + chr2.4:8372065-8372084 MS.gene31325:CDS 50.0%
TTGCATCTGCTCGTAAGTGG+AGG + chr2.4:8370882-8370901 MS.gene31325:CDS 50.0%
! AGTAATGGAAGAAAGCGCGG+CGG + chr2.4:8369700-8369719 MS.gene31325:CDS 50.0%
! CCAGGTTGGCATAAGGTTAC+TGG + chr2.4:8371675-8371694 MS.gene31325:CDS 50.0%
! GCTATTGCCAGGTTGGCATA+AGG + chr2.4:8371668-8371687 MS.gene31325:CDS 50.0%
! GGGATGTCACTTGCAAGCAA+TGG + chr2.4:8369896-8369915 MS.gene31325:CDS 50.0%
! TGTGGCTGCCTTTGTTGGTT+TGG + chr2.4:8370778-8370797 MS.gene31325:CDS 50.0%
!! GCCACATGGAAGTGCGATTT+TGG + chr2.4:8370841-8370860 MS.gene31325:CDS 50.0%
TGTGCTGTGGCTGCCTTTGT+TGG + chr2.4:8370773-8370792 MS.gene31325:CDS 55.0%
AGTGCTGCCTCCGCCAATAC+CGG + chr2.4:8370970-8370989 MS.gene31325:CDS 60.0%
CCTCCGCCAATACCGGGAAA+AGG + chr2.4:8370977-8370996 MS.gene31325:CDS 60.0%
GCAACGATTGCGGCCACTAC+TGG - chr2.4:8370025-8370044 None:intergenic 60.0%
! ATGGAAGAAAGCGCGGCGGT+TGG + chr2.4:8369704-8369723 MS.gene31325:CDS 60.0%
!!! CCTTTTCCCGGTATTGGCGG+AGG - chr2.4:8370980-8370999 None:intergenic 60.0%
GTGCTGCCTCCGCCAATACC+GGG + chr2.4:8370971-8370990 MS.gene31325:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 8369652 8372145 8369652 ID=MS.gene31325
chr2.4 mRNA 8369652 8372145 8369652 ID=MS.gene31325.t1;Parent=MS.gene31325
chr2.4 exon 8369652 8369748 8369652 ID=MS.gene31325.t1.exon1;Parent=MS.gene31325.t1
chr2.4 CDS 8369652 8369748 8369652 ID=cds.MS.gene31325.t1;Parent=MS.gene31325.t1
chr2.4 exon 8369885 8370102 8369885 ID=MS.gene31325.t1.exon2;Parent=MS.gene31325.t1
chr2.4 CDS 8369885 8370102 8369885 ID=cds.MS.gene31325.t1;Parent=MS.gene31325.t1
chr2.4 exon 8370452 8371005 8370452 ID=MS.gene31325.t1.exon3;Parent=MS.gene31325.t1
chr2.4 CDS 8370452 8371005 8370452 ID=cds.MS.gene31325.t1;Parent=MS.gene31325.t1
chr2.4 exon 8371362 8372145 8371362 ID=MS.gene31325.t1.exon4;Parent=MS.gene31325.t1
chr2.4 CDS 8371362 8372145 8371362 ID=cds.MS.gene31325.t1;Parent=MS.gene31325.t1
Gene Sequence

>MS.gene31325

ATGGATGGTCAGGTAGCAGCATCCTCAAAAGAAGCAGATGAGAGCTTGAGTAATGGAAGAAAGCGCGGCGGTTGGATAAGTTTCCCCTTCTTCATTGGTCTCTCTCTTTTTCCCTTTCATTTTTTAACTCATCAACATTATTAATTCACTCACTTTTACACATCAAAATCATTTTGTTTGAGTCTTATTAGATAAATGTTTATATTTTATTTTATACTGTTGATTAACTATAGGTATGATAGCGGGGATGTCACTTGCAAGCAATGGAATAGTGGGAAACTTGATTGTATATCTTACAAGAGAATTCAATATAAAGAGCATCACTGCAACTCAAATTTATAATGTTGTCGTTGGAAGCACCAGTATATTTCCAGTAGTGGCCGCAATCGTTGCTGATTCTTTCTTTGGTTCTTTCTCTGTTGCCTTTGCTTCTTCTTGTATTTTATTGCTGGTAAACTTTAACACTCAATCCAAATTATATTGCATATATGCTAGCATTTTGTTTTGTCACGATAGACTAATAGCTAGTGTGACTAAGATAAATATTTACATCTAATATGGTTAGTCTATTCCATCATCAGTCATCACATATTTCCTCTATTACGTTCTTTTTTTAGAAGTGGTTTTCGAGTAAGTCATTAGAACGTTTGTTTACTTTTTTCAAGTTGGATATTGCATGTTCGAATTTGTGTCCCAGCGTGCCAAATGTCCTATTTTACAATATGATGTGCATTTTCATAACCAAACATACACTTATTTGTGGTAATATCTCTTTTAAACTTTCACCTTTGCACTTGCAGGGCACAATTCTATTATTCTTGACGGCAACTATCAATTCCTTAAAGCCTCATCCATGCAGCAATGGTTCATCCATCACATGCAAACCACCAACAAGAATCCAATACACAGTTCTATACATAAGCATAGTCCTAATATCAATTGGATTTGGTGGTTCACGTTTCACAGCAGCATCACTAGGTGCAAATCAGTTTGACAAACCAGAACATCAAGGAATATTTTTCAACTGGTACTTCTTCACTTTCTATGTTGCTTCAGGAGCTGCATTAACCGGAATTATTTACATCGAAGATAATTTGGGTTGGGCTATAGGATTTGGTATCTGTGCTGTGGCTGCCTTTGTTGGTTTGGTTGTTTTTGTGTTGGGTTATCGATTTTATCGGGCTGAAAAGCCACATGGAAGTGCGATTTTGGATTTGGGACGTGTTTTTGTTGCATCTGCTCGTAAGTGGAGGTGTAAGCTCTCTTCCAGAGTAGAGGATTACTACGGTAGTTTTTCTGGTCATGATGTTTTGATTCAAGTGCTGCCTCCGCCAATACCGGGAAAAGGATTGAGGTACTAAGTATTAACCACACTTATTCTCATAGTTCAAATGTAGGGGAACGTAGTAAGTAACCTTGAATGCAGATAATCTTATTCTCATATAAAAACAACTTATAGCTTATATACAAATGATAGCTGACAGTTTAGGGGTCCAACATTGTTAGGATGTCTATTCTGTTATGTATTGCTTCTGCACTCTCGTTTATGCTTAAAATGAAACTTATAGCTTATATAAAAACAATTTAATTTGATTTTATCTTTTGATATAACAATAACTTATAAATAAACGTCTCATTAAGCTATTTATCCAAAGAATATGCTTGTAAAGTTTTGCTATATATCATGTGCTGATCTATATCCAAATGCAGGTTTTTCAACCGTGCAGCTCTAATAAGTGATGCAGATCTACACTCAGATGGCACAATTAAGAAATCACCATGGAGACTATGCACAATTCAACAAGTGGAAGATTTCAAAAAAATAATTGGAATATTACCACTATGGACTTCAAGTCTATTCCTATCAATTCCAATAGTAATGCAAATGGACTTCATTATACTTCAAGCTTTAGTGATGGATCGTTCATTAGGATCCCATTTTAAAATCCCAGCTGGTTCTATTTCTGTTATAGTCTTAATATCAGCACCCATTTTCCTCACTTTTCTTGACAGGATGCTATTGCCAGGTTGGCATAAGGTTACTGGAAAATCGCCTAAGCTGCTCCAACAAATAGGAGTAGGACATGTGCTGAATGTTCTTGGCATGGTGGTCTCTGCTCTTGTTGAATCGAAGAGACTCAAATTAGCACGTGTGCATGATGTTTCAATGTCTGTGTTATGGTTGTTTCCACAATTAGTGTTGGTTGGTATTGGAGAAGCTTTTCATTTTCCAGGACAAGTTACATTCTATTATCAACAATTTCCACAATCACTAAAGAGCACATCAACTGCAGCGGTTTCAATGCTTATAGGGATTGCTTTTTACTTGAGCAATGCTTTAATTGACCAGGTTCATAGGAGTACTGATTGGCTACCTGATGACATAAATCACGGGAAGATCGACAATGTGTACTGGATGTTAGTCGTACTCGGGGGTATCAACTTTGTGTATTACTTATTTTGTTCTTATGCTTACAAGTATGAAAATGTTTAA
Protein sequence

>MS.gene31325.t1

MDGQVAASSKEADESLSNGRKRGGWISFPFFIGMIAGMSLASNGIVGNLIVYLTREFNIKSITATQIYNVVVGSTSIFPVVAAIVADSFFGSFSVAFASSCILLLGTILLFLTATINSLKPHPCSNGSSITCKPPTRIQYTVLYISIVLISIGFGGSRFTAASLGANQFDKPEHQGIFFNWYFFTFYVASGAALTGIIYIEDNLGWAIGFGICAVAAFVGLVVFVLGYRFYRAEKPHGSAILDLGRVFVASARKWRCKLSSRVEDYYGSFSGHDVLIQVLPPPIPGKGLRFFNRAALISDADLHSDGTIKKSPWRLCTIQQVEDFKKIIGILPLWTSSLFLSIPIVMQMDFIILQALVMDRSLGSHFKIPAGSISVIVLISAPIFLTFLDRMLLPGWHKVTGKSPKLLQQIGVGHVLNVLGMVVSALVESKRLKLARVHDVSMSVLWLFPQLVLVGIGEAFHFPGQVTFYYQQFPQSLKSTSTAAVSMLIGIAFYLSNALIDQVHRSTDWLPDDINHGKIDNVYWMLVVLGGINFVYYLFCSYAYKYENV