AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31324

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31324.t1 MTR_2g093160 87.636 550 61 3 1 544 1 549 0.0 953
MS.gene31324.t1 MTR_2g093180 84.909 550 77 2 1 544 1 550 0.0 897
MS.gene31324.t1 MTR_2g093640 81.967 549 94 1 1 544 1 549 0.0 891
MS.gene31324.t1 MTR_4g107530 35.612 556 318 9 19 543 36 582 2.86e-106 331
MS.gene31324.t1 MTR_5g038060 35.072 556 335 8 10 542 25 577 8.35e-105 327
MS.gene31324.t1 MTR_4g107580 35.060 579 330 14 1 542 4 573 2.10e-104 326
MS.gene31324.t1 MTR_4g107510 34.768 581 329 14 2 544 18 586 5.52e-103 322
MS.gene31324.t1 MTR_8g469480 34.489 548 335 8 19 544 41 586 6.76e-101 317
MS.gene31324.t1 MTR_3g112460 32.731 553 347 7 2 540 9 550 2.47e-96 304
MS.gene31324.t1 MTR_2g101590 33.573 557 330 12 11 539 21 565 1.47e-95 304
MS.gene31324.t1 MTR_4g011280 32.981 567 337 12 4 539 15 569 3.16e-95 302
MS.gene31324.t1 MTR_2g101610 32.916 559 335 12 9 539 20 566 1.05e-94 302
MS.gene31324.t1 MTR_4g101380 34.574 564 323 12 13 543 24 574 1.21e-94 300
MS.gene31324.t1 MTR_4g107610 32.724 547 343 7 6 532 21 562 6.64e-94 299
MS.gene31324.t1 MTR_7g067010 31.439 563 357 8 2 544 10 563 1.22e-93 297
MS.gene31324.t1 MTR_5g055000 32.491 554 344 8 14 544 16 562 1.48e-93 297
MS.gene31324.t1 MTR_7g088825 31.560 564 355 11 2 542 10 565 2.91e-93 297
MS.gene31324.t1 MTR_3g112470 32.188 553 350 7 2 540 9 550 4.51e-93 295
MS.gene31324.t1 MTR_3g462710 31.681 565 353 10 2 543 10 564 7.20e-93 295
MS.gene31324.t1 MTR_2g101650 34.225 561 331 13 11 544 19 568 8.23e-93 295
MS.gene31324.t1 MTR_8g469470 32.105 570 354 11 2 543 18 582 1.97e-91 292
MS.gene31324.t1 MTR_4g015080 30.919 566 356 10 2 544 20 573 3.15e-90 288
MS.gene31324.t1 MTR_4g114340 34.124 548 330 9 13 540 21 557 4.47e-90 288
MS.gene31324.t1 MTR_2g101640 32.810 573 340 13 1 539 1 562 5.40e-90 289
MS.gene31324.t1 MTR_5g012290 33.155 561 338 12 10 543 21 571 5.73e-90 288
MS.gene31324.t1 MTR_2g101560 33.214 560 332 11 9 539 20 566 9.30e-90 289
MS.gene31324.t1 MTR_5g012270 32.021 559 341 10 13 543 26 573 3.52e-89 286
MS.gene31324.t1 MTR_5g038190 30.035 566 366 10 1 542 9 568 9.70e-89 285
MS.gene31324.t1 MTR_1g057460 33.452 562 327 14 3 539 25 564 1.42e-87 281
MS.gene31324.t1 MTR_3g069500 32.613 555 349 11 2 544 21 562 3.51e-87 280
MS.gene31324.t1 MTR_8g103233 32.558 559 338 12 8 539 7 553 6.74e-87 280
MS.gene31324.t1 MTR_7g088790 31.150 565 360 10 1 542 14 572 7.54e-86 278
MS.gene31324.t1 MTR_7g088820 30.824 558 357 10 8 542 17 568 1.36e-85 277
MS.gene31324.t1 MTR_1g026740 32.437 558 325 18 6 529 4 543 3.42e-85 275
MS.gene31324.t1 MTR_3g112450 32.044 543 344 9 13 540 20 552 6.80e-83 269
MS.gene31324.t1 MTR_4g033325 33.627 568 319 14 1 543 16 550 1.15e-82 268
MS.gene31324.t1 MTR_1g042310 29.307 563 366 9 2 542 12 564 5.05e-82 267
MS.gene31324.t1 MTR_7g098180 29.453 567 365 9 2 544 17 572 5.94e-82 267
MS.gene31324.t1 MTR_7g098220 30.212 566 361 8 2 544 16 570 7.72e-82 267
MS.gene31324.t1 MTR_3g069440 31.383 564 354 8 2 544 19 570 8.14e-82 267
MS.gene31324.t1 MTR_7g098160 28.497 572 359 10 2 542 19 571 8.19e-82 267
MS.gene31324.t1 MTR_1g026750 32.428 552 334 12 14 539 16 554 1.02e-81 266
MS.gene31324.t1 MTR_4g019580 32.000 550 343 10 14 544 34 571 1.17e-81 266
MS.gene31324.t1 MTR_7g098040 27.482 564 375 8 2 542 20 572 1.31e-81 267
MS.gene31324.t1 MTR_1g057460 33.204 515 303 13 44 539 1 493 1.52e-81 263
MS.gene31324.t1 MTR_1g009200 32.310 554 333 11 14 539 25 564 2.68e-81 265
MS.gene31324.t1 MTR_7g064780 31.868 546 350 8 8 533 18 561 4.68e-81 265
MS.gene31324.t1 MTR_7g098090 28.647 569 367 9 2 542 19 576 1.02e-80 264
MS.gene31324.t1 MTR_7g065080 31.009 545 349 10 14 544 29 560 2.94e-80 262
MS.gene31324.t1 MTR_7g098200 29.123 570 360 9 2 543 15 568 3.67e-80 263
MS.gene31324.t1 MTR_7g098200 29.204 565 356 9 2 543 15 558 1.30e-79 261
MS.gene31324.t1 MTR_0294s0020 32.804 567 325 11 1 543 16 550 1.80e-79 259
MS.gene31324.t1 MTR_3g014790 30.458 568 331 11 10 543 11 548 4.01e-79 259
MS.gene31324.t1 MTR_1g075440 28.947 570 359 9 2 542 14 566 9.29e-79 259
MS.gene31324.t1 MTR_4g033315 31.393 567 335 11 1 543 16 552 1.62e-78 257
MS.gene31324.t1 MTR_4g033310 31.393 567 335 11 1 543 16 552 1.62e-78 257
MS.gene31324.t1 MTR_1g026770 31.216 551 339 15 15 539 14 550 1.70e-78 257
MS.gene31324.t1 MTR_5g014150 30.619 565 360 10 1 540 29 586 8.27e-78 256
MS.gene31324.t1 MTR_1g042310 29.658 526 338 9 39 542 5 520 1.53e-77 254
MS.gene31324.t1 MTR_3g072300 30.511 567 355 12 2 543 10 562 1.57e-77 255
MS.gene31324.t1 MTR_1g042310 29.658 526 338 9 39 542 12 527 1.66e-77 254
MS.gene31324.t1 MTR_4g033330 32.294 545 315 11 1 521 16 530 2.23e-77 253
MS.gene31324.t1 MTR_7g098220 30.624 529 333 8 39 544 9 526 3.92e-77 253
MS.gene31324.t1 MTR_7g088820 31.028 506 322 9 59 542 6 506 5.25e-77 253
MS.gene31324.t1 MTR_3g462710 32.613 463 279 10 104 543 3 455 5.72e-77 250
MS.gene31324.t1 MTR_7g005930 29.779 544 358 8 13 540 32 567 6.44e-77 254
MS.gene31324.t1 MTR_1g041875 28.119 537 361 8 2 523 15 541 7.03e-76 251
MS.gene31324.t1 MTR_0236s0050 32.039 515 309 13 44 539 1 493 9.76e-76 248
MS.gene31324.t1 MTR_1g041815 28.873 568 361 9 2 542 13 564 1.38e-75 250
MS.gene31324.t1 MTR_4g033375 32.877 511 292 10 1 491 17 496 2.66e-75 247
MS.gene31324.t1 MTR_2g093650 68.984 187 16 2 334 520 34 178 5.84e-75 237
MS.gene31324.t1 MTR_7g065080 31.340 485 306 9 74 544 2 473 4.19e-74 244
MS.gene31324.t1 MTR_1g041835 27.817 568 367 9 2 542 13 564 5.14e-74 246
MS.gene31324.t1 MTR_5g085850 29.834 543 355 11 13 542 27 556 7.43e-74 246
MS.gene31324.t1 MTR_4g033395 32.554 513 293 11 1 492 17 497 9.71e-74 243
MS.gene31324.t1 MTR_3g069420 29.966 584 356 11 1 544 1 571 1.07e-73 245
MS.gene31324.t1 MTR_4g098800 29.877 569 367 12 1 543 21 583 1.15e-73 245
MS.gene31324.t1 MTR_7g098230 28.330 533 338 9 39 543 49 565 1.36e-73 245
MS.gene31324.t1 MTR_7g064800 30.490 551 357 8 12 536 22 572 2.71e-73 244
MS.gene31324.t1 MTR_4g098800 29.877 569 367 12 1 543 86 648 4.04e-73 245
MS.gene31324.t1 MTR_7g013270 29.391 558 362 9 1 540 81 624 3.09e-72 243
MS.gene31324.t1 MTR_8g038880 30.481 561 332 11 15 544 19 552 6.47e-72 240
MS.gene31324.t1 MTR_3g005010 29.350 569 339 11 1 544 15 545 1.16e-71 239
MS.gene31324.t1 MTR_7g010820 29.720 572 359 9 1 541 11 570 3.26e-71 239
MS.gene31324.t1 MTR_1g116930 29.369 555 364 10 8 544 15 559 1.12e-70 237
MS.gene31324.t1 MTR_1g041885 28.287 502 335 8 39 525 5 496 7.62e-70 234
MS.gene31324.t1 MTR_2g005130 28.597 556 366 8 1 542 25 563 9.27e-70 235
MS.gene31324.t1 MTR_7g088820 31.155 459 287 10 107 542 6 458 1.99e-69 231
MS.gene31324.t1 MTR_3g100980 29.002 531 327 12 43 544 43 552 1.86e-68 231
MS.gene31324.t1 MTR_5g038380 27.556 577 366 13 2 542 14 574 3.42e-68 230
MS.gene31324.t1 MTR_4g033325 33.857 446 247 13 123 543 4 426 3.03e-67 224
MS.gene31324.t1 MTR_4g033325 33.547 468 253 14 101 543 18 452 5.23e-67 224
MS.gene31324.t1 MTR_7g098150 28.959 442 294 6 2 434 19 449 7.78e-67 224
MS.gene31324.t1 MTR_8g469310 27.788 565 365 13 9 542 19 571 7.97e-67 227
MS.gene31324.t1 MTR_2g017750 28.214 560 364 11 16 542 34 588 2.80e-66 226
MS.gene31324.t1 MTR_7g098200 29.701 468 285 9 104 543 3 454 2.69e-65 220
MS.gene31324.t1 MTR_7g111260 28.169 568 355 14 7 543 2 547 6.30e-64 219
MS.gene31324.t1 MTR_4g107670 26.712 584 365 14 2 543 10 572 2.61e-63 217
MS.gene31324.t1 MTR_3g100980 28.086 559 324 14 43 544 43 580 1.27e-62 216
MS.gene31324.t1 MTR_4g123537 27.257 565 373 11 2 543 22 571 1.49e-62 216
MS.gene31324.t1 MTR_4g033385 32.432 444 251 11 127 543 32 453 3.12e-62 211
MS.gene31324.t1 MTR_8g087780 26.620 571 388 9 1 542 6 574 6.15e-62 214
MS.gene31324.t1 MTR_8g087810 28.147 572 379 10 1 542 6 575 1.77e-61 213
MS.gene31324.t1 MTR_1g085090 27.444 532 348 11 33 543 33 547 1.07e-59 208
MS.gene31324.t1 MTR_7g006540 27.273 561 369 12 13 542 60 612 7.12e-58 204
MS.gene31324.t1 MTR_8g091265 70.992 131 33 2 285 410 3 133 2.05e-54 180
MS.gene31324.t1 MTR_4g024930 26.690 562 359 14 2 540 22 553 4.12e-48 176
MS.gene31324.t1 MTR_7g098210 26.718 524 280 11 39 543 5 443 3.78e-47 171
MS.gene31324.t1 MTR_7g051880 24.908 542 360 11 10 539 19 525 2.13e-45 167
MS.gene31324.t1 MTR_6g008690 24.214 541 336 12 39 540 30 535 2.43e-44 165
MS.gene31324.t1 MTR_7g051830 24.170 542 364 10 10 539 19 525 3.29e-44 164
MS.gene31324.t1 MTR_7g052600 24.364 550 369 11 2 539 11 525 1.13e-42 160
MS.gene31324.t1 MTR_1g009170 26.866 402 224 11 144 525 9 360 4.49e-34 133
MS.gene31324.t1 MTR_4g033310 28.707 317 191 7 244 543 190 488 7.67e-34 134
MS.gene31324.t1 MTR_4g033310 34.392 189 107 4 1 184 16 192 4.33e-19 90.9
MS.gene31324.t1 MTR_8g091275 62.500 72 26 1 192 262 1 72 2.68e-25 100
MS.gene31324.t1 MTR_8g091480 66.667 57 14 1 228 284 38 89 9.82e-16 80.9
MS.gene31324.t1 MTR_3g065350 32.258 155 96 4 326 474 1 152 1.40e-12 66.2
MS.gene31324.t1 MTR_7g064160 32.258 93 63 0 8 100 21 113 4.62e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31324.t1 AT3G45650 50.998 551 249 5 2 544 8 545 0.0 565
MS.gene31324.t1 AT3G45660 50.457 547 250 5 6 544 32 565 0.0 559
MS.gene31324.t1 AT3G45660 50.457 547 250 5 6 544 11 544 0.0 558
MS.gene31324.t1 AT3G45660 50.457 547 250 5 6 544 11 544 0.0 558
MS.gene31324.t1 AT3G45650 48.700 577 249 6 2 544 8 571 0.0 550
MS.gene31324.t1 AT3G45680 49.623 530 263 2 15 544 19 544 0.0 536
MS.gene31324.t1 AT3G45710 47.243 544 281 4 2 544 8 546 0.0 533
MS.gene31324.t1 AT3G45700 48.491 530 263 3 15 544 15 534 0.0 524
MS.gene31324.t1 AT3G45720 45.065 537 290 2 8 544 11 542 1.62e-171 497
MS.gene31324.t1 AT3G45690 45.269 539 248 9 7 544 9 501 3.30e-158 461
MS.gene31324.t1 AT1G69870 36.166 553 328 8 11 542 46 594 8.49e-113 348
MS.gene31324.t1 AT1G18880 36.049 577 320 11 4 544 5 568 1.91e-108 336
MS.gene31324.t1 AT3G47960 35.420 559 312 12 19 542 64 608 6.27e-103 323
MS.gene31324.t1 AT1G27080 33.151 549 347 7 8 542 6 548 2.00e-98 310
MS.gene31324.t1 AT5G62680 34.234 555 326 10 19 542 46 592 6.44e-98 310
MS.gene31324.t1 AT2G26690 34.973 549 329 10 10 540 18 556 7.55e-98 308
MS.gene31324.t1 AT1G62200 33.333 552 342 9 2 535 38 581 8.05e-96 303
MS.gene31324.t1 AT5G01180 31.616 563 357 9 2 544 11 565 4.00e-95 301
MS.gene31324.t1 AT5G01180 31.616 563 357 9 2 544 11 565 4.00e-95 301
MS.gene31324.t1 AT5G01180 31.616 563 357 9 2 544 11 565 4.00e-95 301
MS.gene31324.t1 AT3G16180 32.613 555 339 11 12 539 19 565 1.29e-94 300
MS.gene31324.t1 AT1G52190 32.389 565 342 12 7 539 10 566 2.73e-94 300
MS.gene31324.t1 AT3G54140 31.373 561 358 9 2 542 10 563 7.10e-94 298
MS.gene31324.t1 AT3G54140 31.373 561 358 9 2 542 10 563 7.10e-94 298
MS.gene31324.t1 AT2G26690 34.840 531 318 10 28 540 3 523 1.61e-91 291
MS.gene31324.t1 AT1G62200 32.241 580 340 11 2 535 38 610 3.11e-91 292
MS.gene31324.t1 AT1G62200 32.241 580 340 11 2 535 52 624 3.36e-91 293
MS.gene31324.t1 AT1G27080 33.008 512 323 7 45 542 1 506 7.44e-91 289
MS.gene31324.t1 AT2G26690 35.000 520 310 10 39 540 2 511 9.91e-91 288
MS.gene31324.t1 AT1G68570 34.120 551 336 8 17 544 24 570 4.27e-90 288
MS.gene31324.t1 AT1G68570 34.405 529 320 8 39 544 16 540 8.91e-88 281
MS.gene31324.t1 AT3G54450 28.697 568 353 8 5 544 1 544 8.72e-85 273
MS.gene31324.t1 AT5G28470 30.991 555 358 11 8 543 9 557 4.51e-84 272
MS.gene31324.t1 AT2G02040 29.787 564 368 8 2 544 27 583 6.14e-84 272
MS.gene31324.t1 AT5G28470 30.199 553 363 9 8 543 9 555 1.31e-82 268
MS.gene31324.t1 AT5G46040 30.018 543 349 7 2 523 13 545 2.28e-80 263
MS.gene31324.t1 AT1G69860 30.769 559 362 9 1 543 1 550 1.51e-79 260
MS.gene31324.t1 AT1G12110 31.655 556 352 9 10 543 21 570 3.79e-79 259
MS.gene31324.t1 AT2G02020 29.859 566 326 10 2 544 28 545 4.72e-79 258
MS.gene31324.t1 AT3G21670 31.801 544 346 12 13 542 26 558 3.24e-78 257
MS.gene31324.t1 AT3G53960 29.837 553 354 11 14 544 35 575 6.48e-76 251
MS.gene31324.t1 AT2G37900 29.443 557 369 9 1 544 23 568 2.37e-75 249
MS.gene31324.t1 AT1G32450 30.195 563 366 10 2 540 23 582 3.35e-74 247
MS.gene31324.t1 AT2G40460 30.123 571 346 13 2 543 10 556 3.64e-74 246
MS.gene31324.t1 AT1G27040 29.159 559 352 11 9 541 16 556 1.18e-72 242
MS.gene31324.t1 AT1G27040 28.829 555 359 9 9 541 20 560 1.26e-72 242
MS.gene31324.t1 AT5G46050 28.571 539 352 9 2 518 13 540 1.59e-72 242
MS.gene31324.t1 AT1G27040 30.038 526 324 11 42 541 21 528 1.27e-71 238
MS.gene31324.t1 AT4G21680 29.754 568 362 13 2 541 14 572 8.11e-71 238
MS.gene31324.t1 AT1G33440 30.515 544 352 9 15 542 37 570 1.18e-70 238
MS.gene31324.t1 AT1G69850 30.688 567 349 13 14 544 23 581 8.02e-70 235
MS.gene31324.t1 AT5G19640 27.818 550 374 8 2 540 45 582 1.43e-69 235
MS.gene31324.t1 AT1G72125 30.838 561 329 13 14 544 27 558 3.65e-68 230
MS.gene31324.t1 AT2G37900 29.600 500 328 9 58 544 1 489 4.43e-68 228
MS.gene31324.t1 AT1G22540 31.418 557 326 11 9 539 23 549 4.91e-68 229
MS.gene31324.t1 AT1G22550 29.623 557 343 9 14 544 28 561 7.64e-67 226
MS.gene31324.t1 AT1G59740 28.650 548 359 10 10 536 35 571 8.88e-67 227
MS.gene31324.t1 AT1G22570 30.540 537 317 12 14 520 28 538 1.25e-66 226
MS.gene31324.t1 AT1G72130 30.364 550 327 11 11 541 20 532 4.29e-66 224
MS.gene31324.t1 AT1G72140 27.758 562 334 11 14 543 32 553 1.03e-64 221
MS.gene31324.t1 AT5G46050 29.977 437 275 7 103 518 2 428 1.08e-63 216
MS.gene31324.t1 AT1G22570 29.962 524 311 12 27 520 4 501 1.23e-62 214
MS.gene31324.t1 AT1G72120 29.696 559 334 10 14 544 27 554 2.49e-62 214
MS.gene31324.t1 AT3G25260 27.485 513 311 7 43 539 48 515 2.07e-56 197
MS.gene31324.t1 AT5G62730 26.415 583 350 11 2 543 42 586 9.22e-56 197
MS.gene31324.t1 AT1G72130 30.485 433 256 9 128 541 8 414 3.32e-55 192
MS.gene31324.t1 AT1G72130 30.485 433 256 9 128 541 8 414 3.32e-55 192
MS.gene31324.t1 AT5G13400 26.476 559 374 11 15 544 63 613 5.17e-55 196
MS.gene31324.t1 AT5G62730 27.306 542 315 11 43 543 24 527 7.06e-55 194
MS.gene31324.t1 AT5G14940 26.408 568 344 13 14 540 5 539 6.36e-53 189
MS.gene31324.t1 AT5G11570 27.612 536 292 13 22 539 19 476 5.36e-49 176
MS.gene31324.t1 AT3G25280 25.098 510 333 9 43 539 48 521 2.60e-45 167
MS.gene31324.t1 AT3G01350 24.727 550 327 9 39 535 30 545 1.56e-43 163
MS.gene31324.t1 AT2G02020 30.682 264 175 2 2 260 28 288 2.46e-35 139
MS.gene31324.t1 AT2G02020 33.486 218 122 7 345 544 312 524 2.05e-25 110
MS.gene31324.t1 AT2G38100 25.524 525 322 15 44 541 2 484 2.88e-30 124
MS.gene31324.t1 AT2G38100 25.524 525 322 15 44 541 18 500 4.96e-30 124
MS.gene31324.t1 AT2G38100 25.524 525 322 15 44 541 24 506 7.26e-30 123

Find 103 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAATTTGGGTTGGGCTTT+GGG 0.135390 2.4:+8365427 MS.gene31324:CDS
CGGAGGAATTGTGGGAAATT+TGG 0.175787 2.4:+8364817 MS.gene31324:CDS
TTGGGTTGGGCTTTGGGGTT+TGG 0.178493 2.4:+8365433 MS.gene31324:CDS
ATCGTTGCTGATTCTTTCTT+TGG 0.186929 2.4:+8364941 MS.gene31324:CDS
TTCACATTCTATGTTGCTTC+TGG 0.199343 2.4:+8365373 MS.gene31324:CDS
GGATAATTTGGGTTGGGCTT+TGG 0.200907 2.4:+8365426 MS.gene31324:CDS
TTCTTCTTGTGTTGCTTTGC+TGG 0.209226 2.4:+8364985 MS.gene31324:CDS
TTCTTCTGGTCATGATGTTT+TGG 0.260860 2.4:+8365630 MS.gene31324:CDS
CCAGGTTGGCATAAGATTAC+TGG 0.280111 2.4:+8366237 MS.gene31324:CDS
ATAAGTTTCCCTTTCTTCAT+TGG 0.280778 2.4:+8364328 MS.gene31324:CDS
GCTACAAGGAAGTGCAGTTT+TGG 0.285342 2.4:+8365528 MS.gene31324:CDS
GTCCTAATATCAATTGGTTT+TGG 0.302104 2.4:+8365265 MS.gene31324:CDS
CAATGATTTCAATGATTATA+GGG 0.302871 2.4:+8366502 MS.gene31324:CDS
GATTACTATGGTAGTTCTTC+TGG 0.306973 2.4:+8365616 MS.gene31324:CDS
GCAATGATTTCAATGATTAT+AGG 0.313595 2.4:+8366501 MS.gene31324:CDS
GTCAAGAAATGTGAGGAAAA+TGG 0.324649 2.4:-8366202 None:intergenic
CAATTAGTGTTGGTTGGTAT+TGG 0.327167 2.4:+8366402 MS.gene31324:CDS
TTAGTCATGGATCGTTCTTT+AGG 0.329015 2.4:+8366123 MS.gene31324:CDS
CACCAAAACCAATTGATATT+AGG 0.344369 2.4:-8365267 None:intergenic
TACTGGATGTTAGTAGTGTT+TGG 0.345072 2.4:+8366621 MS.gene31324:CDS
ATGTTTCTATGTCTGTGTTA+TGG 0.348978 2.4:+8366370 MS.gene31324:CDS
CATTGAGGATAATTTGGGTT+GGG 0.354468 2.4:+8365420 MS.gene31324:CDS
TTGCCATGATTTATTTCATC+AGG 0.374654 2.4:-8366585 None:intergenic
CAGATTGTAAACATTGTTAT+CGG 0.381247 2.4:+8364887 MS.gene31324:CDS
TAACACCGGGAAAAGGATTA+AGG 0.383763 2.4:+8365671 MS.gene31324:CDS
GAAGTACAGCCAAGCTACTT+TGG 0.393641 2.4:-8366095 None:intergenic
GGACATGTGTTGACTGTTCT+TGG 0.399868 2.4:+8366294 MS.gene31324:CDS
GGATTGCTATAGGTGTTGAT+AGG 0.405717 2.4:-8366074 None:intergenic
AAGCTACTTTGGATTGCTAT+AGG 0.417851 2.4:-8366084 None:intergenic
AGCATAGTCCTAATATCAAT+TGG 0.421222 2.4:+8365259 MS.gene31324:CDS
CTTGACAGGGTGCTATTGCC+AGG 0.421420 2.4:+8366219 MS.gene31324:CDS
ATAATTTGGGTTGGGCTTTG+GGG 0.424970 2.4:+8365428 MS.gene31324:CDS
GCTTCTGGAGCTGCATTCAC+TGG 0.428108 2.4:+8365388 MS.gene31324:CDS
TAATCAGAATCCTACCACTT+TGG 0.441145 2.4:+8366037 MS.gene31324:CDS
CCTATGCCGCTTCAACGAAT+AGG 0.444488 2.4:+8366267 MS.gene31324:CDS
GAGAGAGACCAATGAAGAAA+GGG 0.452539 2.4:-8364336 None:intergenic
TAATAGAATGTAACTTGTCC+TGG 0.454723 2.4:-8366441 None:intergenic
GGTTAAATAGGTATAGTAGC+AGG 0.465969 2.4:+8364779 MS.gene31324:intron
AGAGAGAGACCAATGAAGAA+AGG 0.466641 2.4:-8364337 None:intergenic
TCCAGAGTAGAGGATTACTA+TGG 0.467175 2.4:+8365604 MS.gene31324:CDS
TTATATATAGAACTGTGTAT+TGG 0.469265 2.4:-8365237 None:intergenic
CCTATTCGTTGAAGCGGCAT+AGG 0.471332 2.4:-8366267 None:intergenic
ATTACATGCAAGCCACCTAC+TGG 0.472106 2.4:+8365211 MS.gene31324:CDS
AATCAGTACTCCTACGAACT+TGG 0.476238 2.4:-8366557 None:intergenic
TTTCCACAATTAGTGTTGGT+TGG 0.482453 2.4:+8366396 MS.gene31324:CDS
CCAGTAATCTTATGCCAACC+TGG 0.485407 2.4:-8366237 None:intergenic
CCTCCGCTAACACCGGGAAA+AGG 0.486201 2.4:+8365664 MS.gene31324:CDS
CGTTTCACTGCAGCATCACT+AGG 0.490573 2.4:+8365295 MS.gene31324:CDS
ACAGGGTGCTATTGCCAGGT+TGG 0.495314 2.4:+8366223 MS.gene31324:CDS
CTTGGAAGCGGAGGAATTGT+GGG 0.495498 2.4:+8364809 MS.gene31324:CDS
TCAAGAAATGTGAGGAAAAT+GGG 0.497572 2.4:-8366201 None:intergenic
ATGACAGAAACAGAACTAGC+TGG 0.499461 2.4:-8366162 None:intergenic
ACTTGGAAGCGGAGGAATTG+TGG 0.504054 2.4:+8364808 MS.gene31324:CDS
TACACTCAGATGGCTCAGTT+AGG 0.509635 2.4:+8365962 MS.gene31324:CDS
AACAATTTCCACAATCACTT+AGG 0.511803 2.4:+8366466 MS.gene31324:CDS
TTGTTGCATCTGTTCGTAAG+TGG 0.518784 2.4:+8365566 MS.gene31324:CDS
TGTGTTGACTGTTCTTGGCA+TGG 0.519665 2.4:+8366299 MS.gene31324:CDS
TTACCTGATGAAATAAATCA+TGG 0.531630 2.4:+8366582 MS.gene31324:CDS
GCAAGTTTGATAATGTGTAC+TGG 0.531871 2.4:+8366604 MS.gene31324:CDS
AAGCATCAATCTATTTCCAC+TGG 0.532384 2.4:+8364910 MS.gene31324:CDS
GTTGTTTCCACAATTAGTGT+TGG 0.534629 2.4:+8366392 MS.gene31324:CDS
ATACCAACCAACACTAATTG+TGG 0.537554 2.4:-8366399 None:intergenic
TCATTGAGGATAATTTGGGT+TGG 0.538543 2.4:+8365419 MS.gene31324:CDS
ATAGTAGCAGGGTCGTCACT+TGG 0.545714 2.4:+8364791 MS.gene31324:CDS
TATCGGGCTGAAAAGCTACA+AGG 0.546688 2.4:+8365514 MS.gene31324:CDS
TTTCCTCACATTTCTTGACA+GGG 0.548202 2.4:+8366206 MS.gene31324:CDS
TATAGCAATCCAAAGTAGCT+TGG 0.554810 2.4:+8366086 MS.gene31324:CDS
AAGTTCGTAGGAGTACTGAT+TGG 0.558833 2.4:+8366559 MS.gene31324:CDS
ACCATAGTAATCCTCTACTC+TGG 0.561531 2.4:-8365605 None:intergenic
GAAACAGATGAGAGCAAGAG+TGG 0.566534 2.4:+8364298 MS.gene31324:CDS
AGTGCTGCCTCCGCTAACAC+CGG 0.566712 2.4:+8365657 MS.gene31324:CDS
TGGATGTTAGTAGTGTTTGG+AGG 0.575496 2.4:+8366624 MS.gene31324:CDS
GCAGGGTCGTCACTTGGAAG+CGG 0.575760 2.4:+8364797 MS.gene31324:CDS
GAATCATTGCTACATGGATG+AGG 0.577520 2.4:-8365184 None:intergenic
GTGCTGCCTCCGCTAACACC+GGG 0.585063 2.4:+8365658 MS.gene31324:CDS
TAACTGAGCCATCTGAGTGT+AGG 0.589021 2.4:-8365960 None:intergenic
TGACAGAAACAGAACTAGCT+GGG 0.591074 2.4:-8366161 None:intergenic
ATGAGAGCAAGAGTGGCGGT+TGG 0.591364 2.4:+8364305 MS.gene31324:CDS
TGCAAGTGTGCAACAATGGA+TGG 0.601310 2.4:+8364253 None:intergenic
GTTAAATAGGTATAGTAGCA+GGG 0.602320 2.4:+8364780 MS.gene31324:intron
CTTTAATCGACCAAGTTCGT+AGG 0.606772 2.4:+8366547 MS.gene31324:CDS
CCGACTCCTATTCGTTGAAG+CGG 0.608007 2.4:-8366273 None:intergenic
TAAGCTCTCATCCAGAGTAG+AGG 0.611738 2.4:+8365594 MS.gene31324:CDS
TTTGACAAACTAGAACATCA+AGG 0.611836 2.4:+8365328 MS.gene31324:CDS
TGTACTTCAAGCTTTAGTCA+TGG 0.614823 2.4:+8366110 MS.gene31324:CDS
GCAACGATTGCAGCAACCAG+TGG 0.621100 2.4:-8364926 None:intergenic
GGGTCGTCACTTGGAAGCGG+AGG 0.621705 2.4:+8364800 MS.gene31324:CDS
GCTGTGCACACTTCAACAAG+TGG 0.625265 2.4:+8365999 MS.gene31324:CDS
GATGCAGACCTACACTCAGA+TGG 0.626629 2.4:+8365952 MS.gene31324:CDS
GCTCAGTTAGGAAATCATCA+TGG 0.632715 2.4:+8365974 MS.gene31324:CDS
ATGAATGAATCATTGCTACA+TGG 0.633109 2.4:-8365190 None:intergenic
GTGTATTGGATTCCAGTAGG+TGG 0.634548 2.4:-8365223 None:intergenic
ACTGTGTATTGGATTCCAGT+AGG 0.641176 2.4:-8365226 None:intergenic
GGGGTTTGGAATTTGTGTCG+TGG 0.643071 2.4:+8365447 MS.gene31324:CDS
TACTTGAAGTCCAAAGTGGT+AGG 0.647026 2.4:-8366047 None:intergenic
AATATACTTGAAGTCCAAAG+TGG 0.648493 2.4:-8366051 None:intergenic
CCGCTTCAACGAATAGGAGT+CGG 0.650057 2.4:+8366273 MS.gene31324:CDS
ACAGATGAGAGCAAGAGTGG+CGG 0.658907 2.4:+8364301 MS.gene31324:CDS
AAGAGACTCAAATTAGCACA+TGG 0.673591 2.4:+8366345 MS.gene31324:CDS
GATGTGCTCCTAAGTGATTG+TGG 0.690361 2.4:-8366474 None:intergenic
CATCAGTTACTAGAGCTGCA+CGG 0.693655 2.4:-8365933 None:intergenic
GCACCCTGTCAAGAAATGTG+AGG 0.706666 2.4:-8366209 None:intergenic
CAGTTAGGAAATCATCATGG+AGG 0.743788 2.4:+8365977 MS.gene31324:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGTAATTTTTAAAAAAAG+TGG - chr2.4:8365710-8365729 None:intergenic 10.0%
!!! ATGATCATATTTTATATTGT+TGG + chr2.4:8364758-8364777 MS.gene31324:intron 15.0%
!!! TTTTATATTGTTGGTTAAAT+AGG + chr2.4:8364767-8364786 MS.gene31324:intron 15.0%
!! AACTTCAAATTACTATATAG+AGG - chr2.4:8364414-8364433 None:intergenic 20.0%
!! ATAGAAAAGTTTGATATGTT+GGG + chr2.4:8364642-8364661 MS.gene31324:intron 20.0%
!! ATCTATATATATGCAAATTC+AGG + chr2.4:8365902-8365921 MS.gene31324:intron 20.0%
!! CAATGATTTCAATGATTATA+GGG + chr2.4:8366502-8366521 MS.gene31324:CDS 20.0%
!! TATAGAAAAGTTTGATATGT+TGG + chr2.4:8364641-8364660 MS.gene31324:intron 20.0%
!! TTATATATAGAACTGTGTAT+TGG - chr2.4:8365240-8365259 None:intergenic 20.0%
!!! AATATGACTAACATAACTTT+TGG - chr2.4:8364462-8364481 None:intergenic 20.0%
!!! ATTTTCATTGAGGATAATTT+GGG + chr2.4:8365415-8365434 MS.gene31324:CDS 20.0%
!!! TATTTTCATTGAGGATAATT+TGG + chr2.4:8365414-8365433 MS.gene31324:CDS 20.0%
! ATGTTAGTCATATTCGAATT+TGG + chr2.4:8364468-8364487 MS.gene31324:intron 25.0%
! CAGATTGTAAACATTGTTAT+CGG + chr2.4:8364887-8364906 MS.gene31324:CDS 25.0%
! GCAATGATTTCAATGATTAT+AGG + chr2.4:8366501-8366520 MS.gene31324:CDS 25.0%
! TAAAAAAAGTGGTTAGTACT+TGG - chr2.4:8365699-8365718 None:intergenic 25.0%
! TAGAAAAGTTTGATATGTTG+GGG + chr2.4:8364643-8364662 MS.gene31324:intron 25.0%
! TATATTAAGTTATTGAGCTC+AGG - chr2.4:8364562-8364581 None:intergenic 25.0%
! TGTTAGTCATATTCGAATTT+GGG + chr2.4:8364469-8364488 MS.gene31324:intron 25.0%
! TTACCTGATGAAATAAATCA+TGG + chr2.4:8366582-8366601 MS.gene31324:CDS 25.0%
! TTGATGAGTTAAAAGATGAA+AGG - chr2.4:8364369-8364388 None:intergenic 25.0%
! TTTATCACATCAATCAAAGT+AGG + chr2.4:8365052-8365071 MS.gene31324:intron 25.0%
!! CTCATCAAAATCATTTTGTT+TGG + chr2.4:8364381-8364400 MS.gene31324:intron 25.0%
!!! ATCAAGGAATATTTTTCAAC+TGG + chr2.4:8365344-8365363 MS.gene31324:CDS 25.0%
!!! TTATTTTGTGAAATCCATGT+GGG + chr2.4:8365731-8365750 MS.gene31324:intron 25.0%
AACAATTTCCACAATCACTT+AGG + chr2.4:8366466-8366485 MS.gene31324:CDS 30.0%
AATATACTTGAAGTCCAAAG+TGG - chr2.4:8366054-8366073 None:intergenic 30.0%
ACCATCTCATATGTACTTAT+GGG - chr2.4:8364614-8364633 None:intergenic 30.0%
AGAAAAGTTTGATATGTTGG+GGG + chr2.4:8364644-8364663 MS.gene31324:intron 30.0%
AGCATAGTCCTAATATCAAT+TGG + chr2.4:8365259-8365278 MS.gene31324:CDS 30.0%
ATAAGTTTCCCTTTCTTCAT+TGG + chr2.4:8364328-8364347 MS.gene31324:CDS 30.0%
ATGAATGAATCATTGCTACA+TGG - chr2.4:8365193-8365212 None:intergenic 30.0%
CACCAAAACCAATTGATATT+AGG - chr2.4:8365270-8365289 None:intergenic 30.0%
CAGACGAATAAGTGTATATT+CGG - chr2.4:8365079-8365098 None:intergenic 30.0%
CGGTAAATGACAAATTGTTT+GGG - chr2.4:8364499-8364518 None:intergenic 30.0%
CTTAAGTAATTGATGAACTC+CGG - chr2.4:8364519-8364538 None:intergenic 30.0%
GTGAATCTCATTACTAAGTA+GGG - chr2.4:8365779-8365798 None:intergenic 30.0%
GTTAAATAGGTATAGTAGCA+GGG + chr2.4:8364780-8364799 MS.gene31324:intron 30.0%
GTTAGTCATATTCGAATTTG+GGG + chr2.4:8364470-8364489 MS.gene31324:intron 30.0%
TACCATCTCATATGTACTTA+TGG - chr2.4:8364615-8364634 None:intergenic 30.0%
TCAAGAAATGTGAGGAAAAT+GGG - chr2.4:8366204-8366223 None:intergenic 30.0%
TGTGAATCTCATTACTAAGT+AGG - chr2.4:8365780-8365799 None:intergenic 30.0%
TTGCCATGATTTATTTCATC+AGG - chr2.4:8366588-8366607 None:intergenic 30.0%
TTTGACAAACTAGAACATCA+AGG + chr2.4:8365328-8365347 MS.gene31324:CDS 30.0%
! ATGTTTCTATGTCTGTGTTA+TGG + chr2.4:8366370-8366389 MS.gene31324:CDS 30.0%
! CACTGGAATTATTTTCATTG+AGG + chr2.4:8365405-8365424 MS.gene31324:CDS 30.0%
! CCAAACAATTTGTCATTTAC+CGG + chr2.4:8364497-8364516 MS.gene31324:intron 30.0%
! TAATAGAATGTAACTTGTCC+TGG - chr2.4:8366444-8366463 None:intergenic 30.0%
! TCAACGCTTACTCTTTTATT+TGG - chr2.4:8365812-8365831 None:intergenic 30.0%
!! AACTAGCTGGGATTTTAAAA+TGG - chr2.4:8366152-8366171 None:intergenic 30.0%
!! GTCCTAATATCAATTGGTTT+TGG + chr2.4:8365265-8365284 MS.gene31324:CDS 30.0%
!! GTTATTTTGTGAAATCCATG+TGG + chr2.4:8365730-8365749 MS.gene31324:intron 30.0%
!!! ACTAGCTGGGATTTTAAAAT+GGG - chr2.4:8366151-8366170 None:intergenic 30.0%
!!! CTAATATCAATTGGTTTTGG+TGG + chr2.4:8365268-8365287 MS.gene31324:CDS 30.0%
!!! TGTTGGGTTATCGATTTTAT+CGG + chr2.4:8365497-8365516 MS.gene31324:CDS 30.0%
AAGAGACTCAAATTAGCACA+TGG + chr2.4:8366345-8366364 MS.gene31324:CDS 35.0%
AAGCATCAATCTATTTCCAC+TGG + chr2.4:8364910-8364929 MS.gene31324:CDS 35.0%
ATACCAACCAACACTAATTG+TGG - chr2.4:8366402-8366421 None:intergenic 35.0%
CATATTCTCCTTTCAATGCT+TGG - chr2.4:8365847-8365866 None:intergenic 35.0%
CCGGTAAATGACAAATTGTT+TGG - chr2.4:8364500-8364519 None:intergenic 35.0%
CGAATATACACTTATTCGTC+TGG + chr2.4:8365077-8365096 MS.gene31324:intron 35.0%
GATTACTATGGTAGTTCTTC+TGG + chr2.4:8365616-8365635 MS.gene31324:CDS 35.0%
GGTTAAATAGGTATAGTAGC+AGG + chr2.4:8364779-8364798 MS.gene31324:intron 35.0%
GTCAAGAAATGTGAGGAAAA+TGG - chr2.4:8366205-8366224 None:intergenic 35.0%
TAATCAGAATCCTACCACTT+TGG + chr2.4:8366037-8366056 MS.gene31324:CDS 35.0%
TACTGGATGTTAGTAGTGTT+TGG + chr2.4:8366621-8366640 MS.gene31324:CDS 35.0%
TATAGCAATCCAAAGTAGCT+TGG + chr2.4:8366086-8366105 MS.gene31324:CDS 35.0%
TCATTGAGGATAATTTGGGT+TGG + chr2.4:8365419-8365438 MS.gene31324:CDS 35.0%
TCCCATAAGTACATATGAGA+TGG + chr2.4:8364610-8364629 MS.gene31324:intron 35.0%
TTAGTCATGGATCGTTCTTT+AGG + chr2.4:8366123-8366142 MS.gene31324:CDS 35.0%
TTTACGCACTAAATGTTGAG+AGG - chr2.4:8364701-8364720 None:intergenic 35.0%
TTTCCTCACATTTCTTGACA+GGG + chr2.4:8366206-8366225 MS.gene31324:CDS 35.0%
! AAGCTACTTTGGATTGCTAT+AGG - chr2.4:8366087-8366106 None:intergenic 35.0%
! CATTGAGGATAATTTGGGTT+GGG + chr2.4:8365420-8365439 MS.gene31324:CDS 35.0%
! GTTGTTTCCACAATTAGTGT+TGG + chr2.4:8366392-8366411 MS.gene31324:CDS 35.0%
! TGTACTTCAAGCTTTAGTCA+TGG + chr2.4:8366110-8366129 MS.gene31324:CDS 35.0%
! TTCACATTCTATGTTGCTTC+TGG + chr2.4:8365373-8365392 MS.gene31324:CDS 35.0%
! TTCTTCTGGTCATGATGTTT+TGG + chr2.4:8365630-8365649 MS.gene31324:CDS 35.0%
! TTTTAACCTTTGCACATGCA+GGG + chr2.4:8365118-8365137 MS.gene31324:intron 35.0%
! TTTTCCTCACATTTCTTGAC+AGG + chr2.4:8366205-8366224 MS.gene31324:CDS 35.0%
!! ATCGTTGCTGATTCTTTCTT+TGG + chr2.4:8364941-8364960 MS.gene31324:CDS 35.0%
!! ATTTTAACCTTTGCACATGC+AGG + chr2.4:8365117-8365136 MS.gene31324:intron 35.0%
!! CAATTAGTGTTGGTTGGTAT+TGG + chr2.4:8366402-8366421 MS.gene31324:CDS 35.0%
!! GAAAGTTACCAAGCATTGAA+AGG + chr2.4:8365836-8365855 MS.gene31324:intron 35.0%
!! GCAAGTTTGATAATGTGTAC+TGG + chr2.4:8366604-8366623 MS.gene31324:CDS 35.0%
!! GGTGTGGTTATTTTCTTGTT+GGG + chr2.4:8365481-8365500 MS.gene31324:CDS 35.0%
!! TGGTGTGGTTATTTTCTTGT+TGG + chr2.4:8365480-8365499 MS.gene31324:CDS 35.0%
!! TTTCCACAATTAGTGTTGGT+TGG + chr2.4:8366396-8366415 MS.gene31324:CDS 35.0%
!!! CTAGCTGGGATTTTAAAATG+GGG - chr2.4:8366150-8366169 None:intergenic 35.0%
!!! GTTGGGTTATCGATTTTATC+GGG + chr2.4:8365498-8365517 MS.gene31324:CDS 35.0%
AATCAGTACTCCTACGAACT+TGG - chr2.4:8366560-8366579 None:intergenic 40.0%
ACCATAGTAATCCTCTACTC+TGG - chr2.4:8365608-8365627 None:intergenic 40.0%
ACTGTGTATTGGATTCCAGT+AGG - chr2.4:8365229-8365248 None:intergenic 40.0%
AGAGAGAGACCAATGAAGAA+AGG - chr2.4:8364340-8364359 None:intergenic 40.0%
ATGACAGAAACAGAACTAGC+TGG - chr2.4:8366165-8366184 None:intergenic 40.0%
CAGTTAGGAAATCATCATGG+AGG + chr2.4:8365977-8365996 MS.gene31324:CDS 40.0%
CTTTAATCGACCAAGTTCGT+AGG + chr2.4:8366547-8366566 MS.gene31324:CDS 40.0%
GAATCATTGCTACATGGATG+AGG - chr2.4:8365187-8365206 None:intergenic 40.0%
GAGAGAGACCAATGAAGAAA+GGG - chr2.4:8364339-8364358 None:intergenic 40.0%
GCTCAGTTAGGAAATCATCA+TGG + chr2.4:8365974-8365993 MS.gene31324:CDS 40.0%
TAACACCGGGAAAAGGATTA+AGG + chr2.4:8365671-8365690 MS.gene31324:CDS 40.0%
TACTTGAAGTCCAAAGTGGT+AGG - chr2.4:8366050-8366069 None:intergenic 40.0%
TCCAGAGTAGAGGATTACTA+TGG + chr2.4:8365604-8365623 MS.gene31324:CDS 40.0%
TGACAGAAACAGAACTAGCT+GGG - chr2.4:8366164-8366183 None:intergenic 40.0%
TGGATGTTAGTAGTGTTTGG+AGG + chr2.4:8366624-8366643 MS.gene31324:CDS 40.0%
TTGTTGCATCTGTTCGTAAG+TGG + chr2.4:8365566-8365585 MS.gene31324:CDS 40.0%
! GGAGAAGCTTTTCATTTTCC+AGG + chr2.4:8366423-8366442 MS.gene31324:CDS 40.0%
! TGGTACCTTAATCCTTTTCC+CGG - chr2.4:8365679-8365698 None:intergenic 40.0%
!! AAGTTCGTAGGAGTACTGAT+TGG + chr2.4:8366559-8366578 MS.gene31324:CDS 40.0%
!! ATAATTTGGGTTGGGCTTTG+GGG + chr2.4:8365428-8365447 MS.gene31324:CDS 40.0%
!! GATAATTTGGGTTGGGCTTT+GGG + chr2.4:8365427-8365446 MS.gene31324:CDS 40.0%
!! GCTCTCATCTGTTTCTTTTG+AGG - chr2.4:8364293-8364312 None:intergenic 40.0%
!! GGATTGCTATAGGTGTTGAT+AGG - chr2.4:8366077-8366096 None:intergenic 40.0%
!! TTCTTCTTGTGTTGCTTTGC+TGG + chr2.4:8364985-8365004 MS.gene31324:CDS 40.0%
!!! AGGAAGTGCAGTTTTGGATT+TGG + chr2.4:8365534-8365553 MS.gene31324:CDS 40.0%
!!! GGAAGTGCAGTTTTGGATTT+GGG + chr2.4:8365535-8365554 MS.gene31324:CDS 40.0%
ACGCACTAAATGTTGAGAGG+CGG - chr2.4:8364698-8364717 None:intergenic 45.0%
ATTACATGCAAGCCACCTAC+TGG + chr2.4:8365211-8365230 MS.gene31324:CDS 45.0%
ATTGTTCCCTGCATGTGCAA+AGG - chr2.4:8365127-8365146 None:intergenic 45.0%
CATCAGTTACTAGAGCTGCA+CGG - chr2.4:8365936-8365955 None:intergenic 45.0%
CCAGTAATCTTATGCCAACC+TGG - chr2.4:8366240-8366259 None:intergenic 45.0%
CGGAGGAATTGTGGGAAATT+TGG + chr2.4:8364817-8364836 MS.gene31324:CDS 45.0%
GAAACAGATGAGAGCAAGAG+TGG + chr2.4:8364298-8364317 MS.gene31324:CDS 45.0%
GAAGTACAGCCAAGCTACTT+TGG - chr2.4:8366098-8366117 None:intergenic 45.0%
GATGTGCTCCTAAGTGATTG+TGG - chr2.4:8366477-8366496 None:intergenic 45.0%
GTGTATTGGATTCCAGTAGG+TGG - chr2.4:8365226-8365245 None:intergenic 45.0%
TAACTGAGCCATCTGAGTGT+AGG - chr2.4:8365963-8365982 None:intergenic 45.0%
TAAGCTCTCATCCAGAGTAG+AGG + chr2.4:8365594-8365613 MS.gene31324:CDS 45.0%
TAATGCGATGTGAACCCACA+TGG - chr2.4:8365748-8365767 None:intergenic 45.0%
TACACTCAGATGGCTCAGTT+AGG + chr2.4:8365962-8365981 MS.gene31324:CDS 45.0%
TATCGGGCTGAAAAGCTACA+AGG + chr2.4:8365514-8365533 MS.gene31324:CDS 45.0%
! CCAGGTTGGCATAAGATTAC+TGG + chr2.4:8366237-8366256 MS.gene31324:CDS 45.0%
!! AATCCTTTTCCCGGTGTTAG+CGG - chr2.4:8365670-8365689 None:intergenic 45.0%
!! GCTACAAGGAAGTGCAGTTT+TGG + chr2.4:8365528-8365547 MS.gene31324:CDS 45.0%
!! GGACATGTGTTGACTGTTCT+TGG + chr2.4:8366294-8366313 MS.gene31324:CDS 45.0%
!! GGATAATTTGGGTTGGGCTT+TGG + chr2.4:8365426-8365445 MS.gene31324:CDS 45.0%
!! TGTGTTGACTGTTCTTGGCA+TGG + chr2.4:8366299-8366318 MS.gene31324:CDS 45.0%
!!! TGTGTCGTGGCTACTTTTGT+TGG + chr2.4:8365460-8365479 MS.gene31324:CDS 45.0%
ACAGATGAGAGCAAGAGTGG+CGG + chr2.4:8364301-8364320 MS.gene31324:CDS 50.0%
ACTTGGAAGCGGAGGAATTG+TGG + chr2.4:8364808-8364827 MS.gene31324:CDS 50.0%
ATAGTAGCAGGGTCGTCACT+TGG + chr2.4:8364791-8364810 MS.gene31324:CDS 50.0%
CCGACTCCTATTCGTTGAAG+CGG - chr2.4:8366276-8366295 None:intergenic 50.0%
CCGCTTCAACGAATAGGAGT+CGG + chr2.4:8366273-8366292 MS.gene31324:CDS 50.0%
CCTATTCGTTGAAGCGGCAT+AGG - chr2.4:8366270-8366289 None:intergenic 50.0%
CGCACTAAATGTTGAGAGGC+GGG - chr2.4:8364697-8364716 None:intergenic 50.0%
CGTTTCACTGCAGCATCACT+AGG + chr2.4:8365295-8365314 MS.gene31324:CDS 50.0%
CTTGGAAGCGGAGGAATTGT+GGG + chr2.4:8364809-8364828 MS.gene31324:CDS 50.0%
GATGCAGACCTACACTCAGA+TGG + chr2.4:8365952-8365971 MS.gene31324:CDS 50.0%
GCACCCTGTCAAGAAATGTG+AGG - chr2.4:8366212-8366231 None:intergenic 50.0%
GCACTAAATGTTGAGAGGCG+GGG - chr2.4:8364696-8364715 None:intergenic 50.0%
GCTGTGCACACTTCAACAAG+TGG + chr2.4:8365999-8366018 MS.gene31324:CDS 50.0%
! CCTATGCCGCTTCAACGAAT+AGG + chr2.4:8366267-8366286 MS.gene31324:CDS 50.0%
! GGGGTTTGGAATTTGTGTCG+TGG + chr2.4:8365447-8365466 MS.gene31324:CDS 50.0%
!!! CGTGGCTACTTTTGTTGGTG+TGG + chr2.4:8365465-8365484 MS.gene31324:CDS 50.0%
ATGAGAGCAAGAGTGGCGGT+TGG + chr2.4:8364305-8364324 MS.gene31324:CDS 55.0%
CTAAATGTTGAGAGGCGGGG+AGG - chr2.4:8364693-8364712 None:intergenic 55.0%
GCAACGATTGCAGCAACCAG+TGG - chr2.4:8364929-8364948 None:intergenic 55.0%
!! ACAGGGTGCTATTGCCAGGT+TGG + chr2.4:8366223-8366242 MS.gene31324:CDS 55.0%
!! CTTGACAGGGTGCTATTGCC+AGG + chr2.4:8366219-8366238 MS.gene31324:CDS 55.0%
!! GCTTCTGGAGCTGCATTCAC+TGG + chr2.4:8365388-8365407 MS.gene31324:CDS 55.0%
!! TTGGGTTGGGCTTTGGGGTT+TGG + chr2.4:8365433-8365452 MS.gene31324:CDS 55.0%
AGTGCTGCCTCCGCTAACAC+CGG + chr2.4:8365657-8365676 MS.gene31324:CDS 60.0%
CCTCCGCTAACACCGGGAAA+AGG + chr2.4:8365664-8365683 MS.gene31324:CDS 60.0%
GCAGGGTCGTCACTTGGAAG+CGG + chr2.4:8364797-8364816 MS.gene31324:CDS 60.0%
!! CCTTTTCCCGGTGTTAGCGG+AGG - chr2.4:8365667-8365686 None:intergenic 60.0%
GGGTCGTCACTTGGAAGCGG+AGG + chr2.4:8364800-8364819 MS.gene31324:CDS 65.0%
GTGCTGCCTCCGCTAACACC+GGG + chr2.4:8365658-8365677 MS.gene31324:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 8364268 8366704 8364268 ID=MS.gene31324
chr2.4 mRNA 8364268 8366704 8364268 ID=MS.gene31324.t1;Parent=MS.gene31324
chr2.4 exon 8364268 8364349 8364268 ID=MS.gene31324.t1.exon1;Parent=MS.gene31324.t1
chr2.4 CDS 8364268 8364349 8364268 ID=cds.MS.gene31324.t1;Parent=MS.gene31324.t1
chr2.4 exon 8364789 8365006 8364789 ID=MS.gene31324.t1.exon2;Parent=MS.gene31324.t1
chr2.4 CDS 8364789 8365006 8364789 ID=cds.MS.gene31324.t1;Parent=MS.gene31324.t1
chr2.4 exon 8365139 8365692 8365139 ID=MS.gene31324.t1.exon3;Parent=MS.gene31324.t1
chr2.4 CDS 8365139 8365692 8365139 ID=cds.MS.gene31324.t1;Parent=MS.gene31324.t1
chr2.4 exon 8365924 8366704 8365924 ID=MS.gene31324.t1.exon4;Parent=MS.gene31324.t1
chr2.4 CDS 8365924 8366704 8365924 ID=cds.MS.gene31324.t1;Parent=MS.gene31324.t1
Gene Sequence

>MS.gene31324

ATGGATGGTAAGCTAGCAGCATCCTCAAAAGAAACAGATGAGAGCAAGAGTGGCGGTTGGATAAGTTTCCCTTTCTTCATTGGTCTCTCTCTCTTTTTCCTTTCATCTTTTAACTCATCAAAATCATTTTGTTTGGTTTTATTCCTCTATATAGTAATTTGAAGTTCAGACGACATGTAAGTAAAGTATGACCAAAAGTTATGTTAGTCATATTCGAATTTGGGGTTTCCCAAACAATTTGTCATTTACCGGAGTTCATCAATTACTTAAGCTTATAAAAGAAACTAATTACCTGAGCTCAATAACTTAATATACACACACGTAAAAAGCATGATCATATTGTCCCATAAGTACATATGAGATGGTAAAAAACTATAGAAAAGTTTGATATGTTGGGGGCGTGTGTTCAAATCTGCAACTTTCCTCCCCGCCTCTCAACATTTAGTGCGTAAATTTCATTGCTAGTCTCATTGTAACAGAAAAAGAAAGCATGATCATATTTTATATTGTTGGTTAAATAGGTATAGTAGCAGGGTCGTCACTTGGAAGCGGAGGAATTGTGGGAAATTTGGTTGTATATCTTATACAAGAATTCAATATAAAGAGCATCATTGCTGCTCAGATTGTAAACATTGTTATCGGAAGCATCAATCTATTTCCACTGGTTGCTGCAATCGTTGCTGATTCTTTCTTTGGCTCTTTCTCTGTTGCATTTGCTTCTTCTTGTGTTGCTTTGCTGGTAAGCTTCAACACTAAGCGAGAATTTTTTAATCATATTTCATATTTTATCACATCAATCAAAGTAGGACCGAATATACACTTATTCGTCTGGTAACAATTCTCTTTTAAATTTTAACCTTTGCACATGCAGGGAACAATTATTTTGTTTTTGACAGCAACGATCGATTCTTTAAAGCCTCATCCATGTAGCAATGATTCATTCATTACATGCAAGCCACCTACTGGAATCCAATACACAGTTCTATATATAAGCATAGTCCTAATATCAATTGGTTTTGGTGGTTCACGTTTCACTGCAGCATCACTAGGAGCAAATCAATTTGACAAACTAGAACATCAAGGAATATTTTTCAACTGGTACTTTTTCACATTCTATGTTGCTTCTGGAGCTGCATTCACTGGAATTATTTTCATTGAGGATAATTTGGGTTGGGCTTTGGGGTTTGGAATTTGTGTCGTGGCTACTTTTGTTGGTGTGGTTATTTTCTTGTTGGGTTATCGATTTTATCGGGCTGAAAAGCTACAAGGAAGTGCAGTTTTGGATTTGGGACGTGTTTTTGTTGCATCTGTTCGTAAGTGGAAGTGTAAGCTCTCATCCAGAGTAGAGGATTACTATGGTAGTTCTTCTGGTCATGATGTTTTGGTTCAAGTGCTGCCTCCGCTAACACCGGGAAAAGGATTAAGGTACCAAGTACTAACCACTTTTTTTAAAAATTACTTAGTTATTTTGTGAAATCCATGTGGGTTCACATCGCATTATAAATTTACCCTACTTAGTAATGAGATTCACATGATTTTTACCAAATAAAAGAGTAAGCGTTGAAAATGAAAGTTACCAAGCATTGAAAGGAGAATATGCTTGTAAATTTCTGCTATATAGTAACAATGTGTTGATCTATATATATGCAAATTCAGGTTCTTCAACCGTGCAGCTCTAGTAACTGATGCAGACCTACACTCAGATGGCTCAGTTAGGAAATCATCATGGAGGCTGTGCACACTTCAACAAGTGGAAGATTTTAAAAAAATAATCAGAATCCTACCACTTTGGACTTCAAGTATATTCCTATCAACACCTATAGCAATCCAAAGTAGCTTGGCTGTACTTCAAGCTTTAGTCATGGATCGTTCTTTAGGACCCCATTTTAAAATCCCAGCTAGTTCTGTTTCTGTCATAGTCTTAATATCAGCACCCATTTTCCTCACATTTCTTGACAGGGTGCTATTGCCAGGTTGGCATAAGATTACTGGAAAAACGCCTATGCCGCTTCAACGAATAGGAGTCGGACATGTGTTGACTGTTCTTGGCATGGTTGTCTCTGCTCTTGTTGAATCAAAGAGACTCAAATTAGCACATGGACATGTTTCTATGTCTGTGTTATGGTTGTTTCCACAATTAGTGTTGGTTGGTATTGGAGAAGCTTTTCATTTTCCAGGACAAGTTACATTCTATTATCAACAATTTCCACAATCACTTAGGAGCACATCAACAGCAATGATTTCAATGATTATAGGGATTGCTTTTTACTTGAGTGCTGCTTTAATCGACCAAGTTCGTAGGAGTACTGATTGGTTACCTGATGAAATAAATCATGGCAAGTTTGATAATGTGTACTGGATGTTAGTAGTGTTTGGAGGTATCAACTTTGTGTATTACTTACTGTGCTCTATTTCTTACAAGTATGAAAATGTTTAA
Protein sequence

>MS.gene31324.t1

MDGKLAASSKETDESKSGGWISFPFFIGIVAGSSLGSGGIVGNLVVYLIQEFNIKSIIAAQIVNIVIGSINLFPLVAAIVADSFFGSFSVAFASSCVALLGTIILFLTATIDSLKPHPCSNDSFITCKPPTGIQYTVLYISIVLISIGFGGSRFTAASLGANQFDKLEHQGIFFNWYFFTFYVASGAAFTGIIFIEDNLGWALGFGICVVATFVGVVIFLLGYRFYRAEKLQGSAVLDLGRVFVASVRKWKCKLSSRVEDYYGSSSGHDVLVQVLPPLTPGKGLRFFNRAALVTDADLHSDGSVRKSSWRLCTLQQVEDFKKIIRILPLWTSSIFLSTPIAIQSSLAVLQALVMDRSLGPHFKIPASSVSVIVLISAPIFLTFLDRVLLPGWHKITGKTPMPLQRIGVGHVLTVLGMVVSALVESKRLKLAHGHVSMSVLWLFPQLVLVGIGEAFHFPGQVTFYYQQFPQSLRSTSTAMISMIIGIAFYLSAALIDQVRRSTDWLPDEINHGKFDNVYWMLVVFGGINFVYYLLCSISYKYENV