AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48608

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48608.t1 MTR_4g033315 89.228 557 60 0 1 557 1 557 0.0 1007
MS.gene48608.t1 MTR_4g033310 89.228 557 60 0 1 557 1 557 0.0 1007
MS.gene48608.t1 MTR_4g033330 94.184 533 31 0 1 533 1 533 0.0 998
MS.gene48608.t1 MTR_0294s0020 87.074 557 70 1 1 557 1 555 0.0 975
MS.gene48608.t1 MTR_4g033325 86.022 558 74 2 1 557 1 555 0.0 972
MS.gene48608.t1 MTR_4g033395 84.739 498 74 2 1 496 1 498 0.0 873
MS.gene48608.t1 MTR_4g033375 84.538 498 75 2 1 496 1 498 0.0 867
MS.gene48608.t1 MTR_4g033325 84.876 443 63 2 116 557 18 457 0.0 781
MS.gene48608.t1 MTR_4g033385 83.521 443 72 1 116 557 16 458 0.0 780
MS.gene48608.t1 MTR_4g033325 84.562 434 63 2 125 557 1 431 0.0 764
MS.gene48608.t1 MTR_3g005010 66.606 545 171 5 13 555 12 547 0.0 715
MS.gene48608.t1 MTR_4g033310 87.789 303 37 0 255 557 191 493 0.0 555
MS.gene48608.t1 MTR_4g033310 91.146 192 17 0 1 192 1 192 6.02e-112 342
MS.gene48608.t1 MTR_8g038880 46.225 543 260 6 30 551 19 550 9.06e-160 468
MS.gene48608.t1 MTR_3g014790 42.707 569 280 11 17 557 5 555 1.94e-155 456
MS.gene48608.t1 MTR_7g067010 40.177 565 313 6 8 551 1 561 9.58e-154 452
MS.gene48608.t1 MTR_7g098200 40.917 567 301 8 17 557 15 573 1.08e-152 451
MS.gene48608.t1 MTR_7g098200 40.787 559 303 7 17 557 15 563 3.68e-151 446
MS.gene48608.t1 MTR_3g072300 40.853 563 302 8 17 555 10 565 6.70e-151 446
MS.gene48608.t1 MTR_4g015080 40.248 564 312 6 10 552 13 572 1.37e-150 444
MS.gene48608.t1 MTR_3g069440 40.575 557 306 9 15 551 17 568 1.92e-149 442
MS.gene48608.t1 MTR_7g098180 40.215 557 308 6 17 551 17 570 7.98e-149 441
MS.gene48608.t1 MTR_3g069500 38.968 562 308 9 10 551 14 560 8.12e-148 437
MS.gene48608.t1 MTR_1g042310 40.575 557 305 8 17 551 12 564 5.93e-147 436
MS.gene48608.t1 MTR_3g462710 40.422 569 306 8 10 552 3 564 3.92e-146 433
MS.gene48608.t1 MTR_1g041835 41.261 555 301 8 17 550 13 563 8.76e-145 430
MS.gene48608.t1 MTR_4g019580 38.489 556 315 6 17 552 22 570 1.13e-144 430
MS.gene48608.t1 MTR_3g069420 38.840 569 318 8 3 551 11 569 2.18e-144 429
MS.gene48608.t1 MTR_7g065080 38.061 557 310 8 17 552 17 559 3.89e-141 421
MS.gene48608.t1 MTR_1g041815 40.288 556 307 8 17 551 13 564 7.89e-140 417
MS.gene48608.t1 MTR_1g075440 38.669 556 315 7 17 550 14 565 7.54e-139 415
MS.gene48608.t1 MTR_7g098040 37.950 556 321 6 17 551 20 572 1.81e-138 415
MS.gene48608.t1 MTR_7g098160 38.309 556 319 6 17 551 19 571 4.15e-138 414
MS.gene48608.t1 MTR_1g042310 40.875 526 285 8 48 551 6 527 2.98e-137 409
MS.gene48608.t1 MTR_7g098090 38.503 561 316 7 17 551 19 576 2.04e-136 409
MS.gene48608.t1 MTR_1g042310 40.727 523 284 8 50 550 1 519 1.05e-135 405
MS.gene48608.t1 MTR_1g041875 38.547 537 297 8 17 531 15 540 3.71e-133 400
MS.gene48608.t1 MTR_7g098230 37.338 541 315 6 38 557 33 570 1.33e-131 396
MS.gene48608.t1 MTR_7g098220 36.691 556 328 6 17 551 16 568 1.77e-127 386
MS.gene48608.t1 MTR_1g041885 40.434 507 269 8 50 534 1 496 1.87e-126 381
MS.gene48608.t1 MTR_5g012270 37.610 569 324 8 12 553 10 574 7.83e-124 376
MS.gene48608.t1 MTR_5g055000 37.746 559 311 10 28 554 15 568 1.56e-122 373
MS.gene48608.t1 MTR_5g012290 38.596 570 317 8 12 553 8 572 3.49e-122 372
MS.gene48608.t1 MTR_3g112470 36.964 560 316 10 9 545 1 546 8.22e-122 370
MS.gene48608.t1 MTR_4g101380 37.866 581 317 9 8 553 4 575 2.54e-121 370
MS.gene48608.t1 MTR_3g112460 36.607 560 318 10 9 545 1 546 3.73e-121 369
MS.gene48608.t1 MTR_6g008690 37.848 539 299 10 36 552 12 536 4.86e-117 357
MS.gene48608.t1 MTR_7g088825 35.791 556 324 9 25 553 16 565 9.28e-117 358
MS.gene48608.t1 MTR_7g098220 36.381 525 310 6 48 551 3 524 8.94e-116 354
MS.gene48608.t1 MTR_7g010820 36.778 571 326 11 10 550 5 570 8.65e-115 353
MS.gene48608.t1 MTR_7g065080 37.243 486 270 8 88 552 1 472 2.11e-114 349
MS.gene48608.t1 MTR_7g088820 34.441 572 330 12 1 541 1 558 9.08e-113 348
MS.gene48608.t1 MTR_7g005930 33.631 559 333 10 23 557 27 571 2.86e-111 343
MS.gene48608.t1 MTR_7g098200 38.544 467 253 8 117 557 1 459 3.00e-110 338
MS.gene48608.t1 MTR_4g114340 39.674 552 304 8 16 545 9 553 1.40e-109 339
MS.gene48608.t1 MTR_7g088790 34.261 575 343 9 9 554 7 575 2.58e-109 340
MS.gene48608.t1 MTR_5g014150 34.549 576 341 10 3 548 16 585 3.24e-108 336
MS.gene48608.t1 MTR_3g112450 35.957 559 319 10 15 548 7 551 7.91e-108 334
MS.gene48608.t1 MTR_7g098150 39.101 445 247 6 17 440 19 460 9.13e-108 332
MS.gene48608.t1 MTR_3g462710 38.745 462 250 8 117 552 1 455 3.66e-106 327
MS.gene48608.t1 MTR_4g098800 34.215 567 332 10 10 546 15 570 4.42e-106 330
MS.gene48608.t1 MTR_4g107670 33.913 575 341 10 10 551 3 571 1.72e-105 328
MS.gene48608.t1 MTR_4g098800 34.215 567 332 10 10 546 80 635 2.15e-105 331
MS.gene48608.t1 MTR_5g038060 34.291 557 324 9 29 551 29 577 1.04e-104 327
MS.gene48608.t1 MTR_5g038380 33.851 579 341 11 11 555 8 578 1.67e-103 323
MS.gene48608.t1 MTR_1g116930 33.274 565 334 11 16 548 1 554 8.66e-103 322
MS.gene48608.t1 MTR_8g469310 34.851 571 331 9 18 555 13 575 1.46e-101 318
MS.gene48608.t1 MTR_7g088820 34.337 498 292 9 73 541 5 496 3.21e-101 316
MS.gene48608.t1 MTR_4g107510 35.036 548 324 9 35 552 40 585 4.40e-101 318
MS.gene48608.t1 MTR_2g017750 32.155 594 358 10 2 555 4 592 1.41e-100 317
MS.gene48608.t1 MTR_7g013270 33.452 562 340 10 12 548 71 623 2.95e-99 314
MS.gene48608.t1 MTR_2g005130 34.347 559 336 10 10 551 19 563 8.39e-99 311
MS.gene48608.t1 MTR_8g469480 34.120 551 321 8 35 551 42 584 6.89e-98 310
MS.gene48608.t1 MTR_5g085850 36.201 558 316 9 17 551 16 556 8.56e-98 309
MS.gene48608.t1 MTR_3g100980 34.058 552 328 11 25 551 10 550 2.18e-97 307
MS.gene48608.t1 MTR_8g087780 33.913 575 343 11 15 554 5 577 2.76e-97 307
MS.gene48608.t1 MTR_4g107580 33.216 566 325 11 21 550 24 572 1.29e-96 306
MS.gene48608.t1 MTR_7g006540 32.675 557 332 11 30 550 62 611 4.14e-96 306
MS.gene48608.t1 MTR_8g469470 32.628 567 333 10 23 550 24 580 1.82e-95 303
MS.gene48608.t1 MTR_8g087810 34.715 579 338 12 15 556 5 580 7.58e-95 301
MS.gene48608.t1 MTR_8g103233 33.272 544 336 10 28 548 14 553 4.23e-94 299
MS.gene48608.t1 MTR_1g026750 33.091 550 329 9 28 548 15 554 4.63e-93 296
MS.gene48608.t1 MTR_1g009200 31.993 572 345 11 4 548 10 564 1.45e-91 293
MS.gene48608.t1 MTR_3g100980 32.586 580 327 13 25 551 10 578 2.25e-91 292
MS.gene48608.t1 MTR_4g123537 33.273 556 332 12 21 552 31 571 8.89e-91 291
MS.gene48608.t1 MTR_4g107530 33.105 583 347 12 6 557 17 587 1.23e-90 291
MS.gene48608.t1 MTR_1g026740 32.169 544 332 10 33 548 19 553 1.26e-90 290
MS.gene48608.t1 MTR_5g038190 30.481 561 350 8 23 551 16 568 3.34e-90 289
MS.gene48608.t1 MTR_1g026770 32.234 546 340 8 27 548 11 550 9.14e-90 287
MS.gene48608.t1 MTR_4g107610 32.072 555 342 7 27 553 27 574 1.92e-89 288
MS.gene48608.t1 MTR_7g111260 32.472 542 339 10 29 553 17 548 1.45e-87 282
MS.gene48608.t1 MTR_2g101650 31.413 538 333 10 40 548 33 563 4.92e-87 281
MS.gene48608.t1 MTR_7g088820 32.816 451 268 9 120 541 4 448 1.17e-85 274
MS.gene48608.t1 MTR_2g101590 32.867 572 343 15 10 550 4 565 1.33e-85 278
MS.gene48608.t1 MTR_4g011280 31.760 551 343 11 25 550 27 569 2.58e-85 276
MS.gene48608.t1 MTR_1g085090 31.475 556 352 12 17 553 3 548 2.94e-85 276
MS.gene48608.t1 MTR_2g101610 30.823 571 357 12 10 550 4 566 1.07e-83 273
MS.gene48608.t1 MTR_7g064780 29.558 565 358 9 17 549 12 568 2.88e-83 271
MS.gene48608.t1 MTR_2g101640 30.458 568 357 11 10 548 4 562 1.68e-82 270
MS.gene48608.t1 MTR_7g098210 32.129 526 257 12 50 555 1 446 4.18e-80 258
MS.gene48608.t1 MTR_1g057460 31.144 533 324 14 40 550 53 564 9.02e-79 259
MS.gene48608.t1 MTR_2g101560 30.648 571 358 13 10 550 4 566 6.37e-78 258
MS.gene48608.t1 MTR_1g057460 30.934 514 312 14 59 550 1 493 8.92e-76 249
MS.gene48608.t1 MTR_7g064800 29.803 557 349 8 27 549 22 570 3.90e-75 249
MS.gene48608.t1 MTR_4g024930 30.144 554 342 15 17 548 22 552 5.24e-74 246
MS.gene48608.t1 MTR_0236s0050 29.883 512 318 13 59 548 1 493 2.68e-71 237
MS.gene48608.t1 MTR_2g093640 31.469 572 333 14 16 552 1 548 7.40e-71 237
MS.gene48608.t1 MTR_2g093160 29.947 571 343 13 16 552 1 548 3.90e-70 235
MS.gene48608.t1 MTR_2g093180 30.314 574 338 12 16 552 1 549 4.45e-67 227
MS.gene48608.t1 MTR_7g051880 28.909 550 356 11 9 548 1 525 1.00e-64 220
MS.gene48608.t1 MTR_7g052600 28.942 539 352 9 17 548 11 525 7.46e-62 213
MS.gene48608.t1 MTR_7g051830 28.102 548 363 9 9 548 1 525 3.87e-61 211
MS.gene48608.t1 MTR_4g033390 88.889 90 10 0 466 555 30 119 1.33e-51 172
MS.gene48608.t1 MTR_1g009170 29.032 403 219 10 141 527 3 354 1.99e-45 165
MS.gene48608.t1 MTR_3g065350 38.509 161 91 4 318 478 1 153 2.95e-29 114
MS.gene48608.t1 MTR_7g064160 27.273 99 72 0 17 115 15 113 2.43e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48608.t1 AT1G22540 63.357 554 191 2 10 556 9 557 0.0 684
MS.gene48608.t1 AT1G72125 57.754 561 224 8 1 552 1 557 0.0 622
MS.gene48608.t1 AT1G72120 57.679 560 222 8 1 552 1 553 0.0 612
MS.gene48608.t1 AT1G22550 55.654 566 229 6 1 552 3 560 0.0 602
MS.gene48608.t1 AT1G72140 55.455 550 215 5 16 551 19 552 0.0 601
MS.gene48608.t1 AT1G22570 55.776 554 221 6 16 552 15 561 0.0 582
MS.gene48608.t1 AT1G22570 55.303 528 212 6 42 552 4 524 0.0 548
MS.gene48608.t1 AT1G72130 52.523 535 226 6 29 555 23 537 0.0 531
MS.gene48608.t1 AT3G54450 43.646 543 276 8 29 551 10 542 6.32e-166 483
MS.gene48608.t1 AT5G01180 39.964 558 309 6 17 552 11 564 1.39e-148 439
MS.gene48608.t1 AT5G01180 39.964 558 309 6 17 552 11 564 1.39e-148 439
MS.gene48608.t1 AT5G01180 39.964 558 309 6 17 552 11 564 1.39e-148 439
MS.gene48608.t1 AT2G02040 39.860 572 312 8 3 548 13 578 3.93e-148 439
MS.gene48608.t1 AT2G37900 41.270 567 291 11 10 551 17 566 4.47e-148 438
MS.gene48608.t1 AT3G54140 39.716 564 313 7 10 550 3 562 9.41e-147 434
MS.gene48608.t1 AT3G54140 39.716 564 313 7 10 550 3 562 9.41e-147 434
MS.gene48608.t1 AT3G53960 39.347 582 310 10 3 551 2 573 1.38e-145 432
MS.gene48608.t1 AT1G72130 50.930 430 184 5 133 555 10 419 8.94e-145 424
MS.gene48608.t1 AT1G72130 50.930 430 184 5 133 555 10 419 8.94e-145 424
MS.gene48608.t1 AT1G62200 40.433 554 287 8 17 541 38 577 1.89e-142 424
MS.gene48608.t1 AT2G40460 39.716 564 315 7 11 556 4 560 2.22e-139 416
MS.gene48608.t1 AT1G62200 38.422 583 287 9 17 541 38 606 8.42e-136 408
MS.gene48608.t1 AT2G02020 40.179 560 292 11 3 550 14 542 1.09e-135 405
MS.gene48608.t1 AT1G62200 38.422 583 287 9 17 541 52 620 1.24e-135 408
MS.gene48608.t1 AT5G46040 38.131 535 308 5 17 531 13 544 2.66e-132 398
MS.gene48608.t1 AT5G46050 37.857 560 323 6 17 555 13 568 4.44e-131 395
MS.gene48608.t1 AT2G37900 41.352 503 253 11 74 551 2 487 3.78e-127 382
MS.gene48608.t1 AT5G14940 39.154 544 297 12 29 548 5 538 2.23e-124 376
MS.gene48608.t1 AT2G02020 37.814 558 287 11 3 550 14 521 2.53e-120 365
MS.gene48608.t1 AT3G01350 38.235 544 300 9 33 546 9 546 1.73e-119 364
MS.gene48608.t1 AT1G69870 37.545 554 309 9 28 551 48 594 1.02e-118 364
MS.gene48608.t1 AT1G68570 38.590 539 302 8 40 551 32 568 4.08e-115 354
MS.gene48608.t1 AT1G68570 38.577 534 299 8 45 551 7 538 1.26e-114 352
MS.gene48608.t1 AT1G32450 34.326 571 338 9 10 548 16 581 1.49e-109 340
MS.gene48608.t1 AT2G26690 37.545 554 312 9 16 545 9 552 4.75e-109 338
MS.gene48608.t1 AT4G21680 35.590 576 336 13 5 550 2 572 7.01e-108 335
MS.gene48608.t1 AT1G12110 34.923 587 324 12 3 553 7 571 8.55e-108 335
MS.gene48608.t1 AT3G21670 36.804 557 313 8 19 551 17 558 4.15e-102 320
MS.gene48608.t1 AT1G52190 33.948 542 320 9 40 548 30 566 9.60e-100 315
MS.gene48608.t1 AT2G26690 37.002 527 298 9 43 545 3 519 5.32e-99 311
MS.gene48608.t1 AT1G18880 33.874 555 318 12 35 551 23 566 3.17e-98 310
MS.gene48608.t1 AT1G27040 32.409 577 354 9 6 557 2 567 3.88e-98 309
MS.gene48608.t1 AT1G59740 33.627 568 339 10 17 557 27 583 8.10e-98 309
MS.gene48608.t1 AT1G27040 32.686 566 345 9 17 557 9 563 1.35e-97 308
MS.gene48608.t1 AT3G16180 33.692 558 328 12 25 548 16 565 3.45e-97 307
MS.gene48608.t1 AT1G33440 34.843 574 336 10 7 555 14 574 6.78e-97 307
MS.gene48608.t1 AT1G69850 32.143 588 355 8 9 557 3 585 2.40e-96 305
MS.gene48608.t1 AT5G46050 36.463 458 266 6 119 555 3 456 2.84e-96 301
MS.gene48608.t1 AT2G26690 36.628 516 293 9 54 545 2 507 2.20e-94 298
MS.gene48608.t1 AT5G62730 31.802 566 357 10 1 550 32 584 7.55e-94 298
MS.gene48608.t1 AT1G27080 33.942 548 318 11 33 551 16 548 8.54e-93 295
MS.gene48608.t1 AT5G13400 31.317 562 352 8 20 550 52 610 1.96e-91 293
MS.gene48608.t1 AT1G27040 32.778 540 327 9 43 557 7 535 2.10e-91 291
MS.gene48608.t1 AT5G62680 32.668 551 332 10 35 551 47 592 3.14e-91 293
MS.gene48608.t1 AT5G19640 31.676 543 324 12 32 548 60 581 1.71e-89 288
MS.gene48608.t1 AT5G62730 31.638 531 340 9 37 551 3 526 9.88e-88 281
MS.gene48608.t1 AT3G47960 32.423 549 334 11 35 551 65 608 1.23e-87 284
MS.gene48608.t1 AT1G27080 33.911 519 299 11 62 551 3 506 1.59e-85 275
MS.gene48608.t1 AT1G69860 32.358 547 323 11 35 552 22 550 4.59e-82 267
MS.gene48608.t1 AT5G28470 30.144 554 350 11 28 553 14 558 2.12e-81 265
MS.gene48608.t1 AT5G28470 29.964 554 349 12 28 553 14 556 7.25e-79 258
MS.gene48608.t1 AT3G25280 32.649 536 328 11 21 548 11 521 1.21e-78 257
MS.gene48608.t1 AT3G25260 32.176 547 328 13 12 548 2 515 6.78e-76 249
MS.gene48608.t1 AT3G45650 29.715 562 344 12 17 552 8 544 6.53e-71 238
MS.gene48608.t1 AT3G45680 30.823 571 337 14 11 552 2 543 1.77e-69 234
MS.gene48608.t1 AT3G45710 29.947 571 332 13 17 552 8 545 4.17e-68 230
MS.gene48608.t1 AT3G45660 30.843 522 308 11 58 552 48 543 2.32e-67 228
MS.gene48608.t1 AT3G45660 30.843 522 308 11 58 552 48 543 2.32e-67 228
MS.gene48608.t1 AT3G45660 30.843 522 308 11 58 552 69 564 4.42e-67 228
MS.gene48608.t1 AT3G45700 30.000 550 327 12 30 552 15 533 7.59e-67 226
MS.gene48608.t1 AT3G45650 28.401 588 344 13 17 552 8 570 3.97e-65 223
MS.gene48608.t1 AT3G45720 30.577 520 304 12 40 530 28 519 2.83e-61 212
MS.gene48608.t1 AT5G11570 26.975 519 305 10 40 548 22 476 8.56e-61 209
MS.gene48608.t1 AT3G45690 28.571 539 292 16 30 540 17 490 7.77e-53 188
MS.gene48608.t1 AT2G38100 28.324 519 309 16 59 550 2 484 6.21e-46 169
MS.gene48608.t1 AT2G38100 28.324 519 309 16 59 550 24 506 1.38e-45 168
MS.gene48608.t1 AT2G38100 28.324 519 309 16 59 550 18 500 1.51e-45 168

Find 117 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGCTTCAACTTTGGTTTA+TGG 0.138154 4.1:-66967688 MS.gene48608:CDS
AACCTTGTGTTCAAGCTTTC+GGG 0.147273 4.1:-66968622 MS.gene48608:CDS
CTTCTAGCTCTGTGCTCTTT+AGG 0.180783 4.1:+66968575 None:intergenic
GCTACAAGTTTCATCTTCTT+TGG 0.186389 4.1:+66967759 None:intergenic
TACTAAGGTTGGTTCCAATT+TGG 0.200350 4.1:-66967708 MS.gene48608:CDS
CTAGAAGCTCATTCTTTAAT+TGG 0.206643 4.1:-66968559 MS.gene48608:CDS
GCTTCCCTCCTTCCTCTCTT+AGG 0.216834 4.1:-66969860 MS.gene48608:CDS
GATAACATTAGTTGGGTTCT+TGG 0.227701 4.1:-66968476 MS.gene48608:CDS
CCCATGCTTCTTAGTTCATT+TGG 0.244157 4.1:+66967238 None:intergenic
TGCTTCTCTTATCTATATAT+TGG 0.248624 4.1:-66969789 MS.gene48608:intron
CTTCGCTTCAAACGATAAAA+TGG 0.249694 4.1:-66967576 MS.gene48608:CDS
ATTAGTTGGGTTCTTGGATT+TGG 0.259493 4.1:-66968470 MS.gene48608:CDS
ACTAAGGTTGGTTCCAATTT+GGG 0.262407 4.1:-66967707 MS.gene48608:CDS
TAGCTTATCAACAACGGATT+TGG 0.272927 4.1:-66968303 MS.gene48608:CDS
GACATATAGGTTTAACATTA+AGG 0.274752 4.1:-66968393 MS.gene48608:CDS
AAACCTTGTGTTCAAGCTTT+CGG 0.280299 4.1:-66968623 MS.gene48608:CDS
TACACGACCAATTCTAATAA+AGG 0.293012 4.1:+66968355 None:intergenic
TCCAATTTGGGCTTCAACTT+TGG 0.295489 4.1:-66967695 MS.gene48608:CDS
CCATGCTTCTTAGTTCATTT+GGG 0.297304 4.1:+66967239 None:intergenic
TTAGGTCCGAATCTGGTTAT+TGG 0.299083 4.1:-66970461 MS.gene48608:CDS
CTGCTAAGAATGTGAATATT+TGG 0.301956 4.1:-66969892 MS.gene48608:CDS
AAGAGTCCCTTTATTAGAAT+TGG 0.302187 4.1:-66968362 MS.gene48608:CDS
ACCAAAGTTGAAGCCCAAAT+TGG 0.319490 4.1:+66967694 None:intergenic
CTCATTGGAACAGTTGCATT+TGG 0.332167 4.1:+66967352 None:intergenic
AGGAATATGTGCTCATAGAT+AGG 0.336290 4.1:+66967532 None:intergenic
ATGGAGAGAACAATATTTCC+TGG 0.340438 4.1:-66967616 MS.gene48608:CDS
AACTTGATATCATATCTAAC+AGG 0.343746 4.1:-66969941 MS.gene48608:CDS
CTACAAGTTTCATCTTCTTT+GGG 0.344371 4.1:+66967760 None:intergenic
GCACTCTATTTGAGTATTGT+TGG 0.352342 4.1:-66967211 MS.gene48608:CDS
CTCCATTGAAGTCCCTTGCT+TGG 0.358040 4.1:+66967633 None:intergenic
GCTCAATTAGCTATTGTTGA+TGG 0.367017 4.1:-66970513 MS.gene48608:CDS
GCGGAGAGGGTATCATATTA+TGG 0.374011 4.1:-66969974 MS.gene48608:CDS
TCATCTTCTTTGGGTCCAAT+TGG 0.385147 4.1:+66967769 None:intergenic
ATCACAATGCTTCAACGAAT+TGG 0.388981 4.1:-66967472 MS.gene48608:CDS
CAACTGTTCCAATGAGTATA+TGG 0.398189 4.1:-66967345 MS.gene48608:CDS
AGATCCGCTTGGTTTATCAT+AGG 0.431139 4.1:-66970438 MS.gene48608:intron
CACAAAGGCACTACCACATA+AGG 0.442991 4.1:-66966998 None:intergenic
AAGGCATTGCTAACACCAAT+TGG 0.443061 4.1:-66967784 MS.gene48608:CDS
ACACGACCAATTCTAATAAA+GGG 0.444617 4.1:+66968356 None:intergenic
CCCGCAGTTAGGTCCGAATC+TGG 0.446760 4.1:-66970468 MS.gene48608:CDS
TACTTGGAACAAGGACATAT+AGG 0.448072 4.1:-66968406 MS.gene48608:CDS
ACTCAGGAGAACAATATGTA+AGG 0.468530 4.1:+66968705 None:intergenic
ATATACAAGATAACATTAGT+TGG 0.471533 4.1:-66968484 MS.gene48608:CDS
GAAGCTCATTCTTTAATTGG+TGG 0.474451 4.1:-66968556 MS.gene48608:CDS
ACAGAGGACAGCCCGCAGTT+AGG 0.476911 4.1:-66970479 MS.gene48608:CDS
ACCAACCACCATATACTCAT+TGG 0.479829 4.1:+66967337 None:intergenic
AGCTTCTAACAGCACTTCGT+TGG 0.483350 4.1:+66970533 None:intergenic
TTGCTTCTTCCACCTCACTA+AGG 0.484443 4.1:+66967737 None:intergenic
GGATCTCCAATAACCAGATT+CGG 0.490538 4.1:+66970455 None:intergenic
GTATCATATTATGGAATTCA+AGG 0.491184 4.1:-66969965 MS.gene48608:CDS
AAGAATGTGAATATTTGGTC+TGG 0.492102 4.1:-66969887 MS.gene48608:CDS
TATACAAGATAACATTAGTT+GGG 0.492477 4.1:-66968483 MS.gene48608:CDS
TTTACATTCTTCACCAAGCA+AGG 0.493614 4.1:-66967646 MS.gene48608:CDS
CTTGCTGGATTAAGTGTGGT+GGG 0.506111 4.1:-66967064 MS.gene48608:CDS
TGATAACTCTTGCTAGTGGC+AGG 0.509128 4.1:+66967512 None:intergenic
AATAAGATTACTTGTGACTC+AGG 0.511919 4.1:+66968689 None:intergenic
GCCCCGAAAGCTTGAACACA+AGG 0.524049 4.1:+66968620 None:intergenic
GCAAAGATGATCGTGAAAGT+TGG 0.529983 4.1:-66967135 MS.gene48608:CDS
GTTGCAGTGCTCTGTTTGAG+TGG 0.530822 4.1:+66969917 None:intergenic
TCACTCTATCTAGTTGCCAT+TGG 0.531734 4.1:-66968659 MS.gene48608:CDS
AGCAAAGGCTGTACTAAGGT+TGG 0.534832 4.1:-66967719 MS.gene48608:CDS
AATCATTGGCTATCCTTATG+TGG 0.535072 4.1:+66966985 None:intergenic
AGGCGTGGAAGTGGCGGAGA+GGG 0.539662 4.1:-66969987 MS.gene48608:CDS
CCCAAATGAACTAAGAAGCA+TGG 0.542573 4.1:-66967239 MS.gene48608:CDS
GAGGGAGGTATGTCAAATCC+AGG 0.544553 4.1:+66967598 None:intergenic
TCTTGCTGGATTAAGTGTGG+TGG 0.544756 4.1:-66967065 MS.gene48608:CDS
CTGGTTATTGGAGATCCGCT+TGG 0.544926 4.1:-66970449 MS.gene48608:CDS
TATTTGAGTATTGTTGGTGT+TGG 0.547399 4.1:-66967205 MS.gene48608:CDS
ATGCTTCAACGAATTGGCAC+TGG 0.548210 4.1:-66967466 MS.gene48608:CDS
TCCAATGAGTATATGGTGGT+TGG 0.556157 4.1:-66967338 MS.gene48608:CDS
GGAATATGTGCTCATAGATA+GGG 0.557230 4.1:+66967533 None:intergenic
GGAGAACAATATGTAAGGGA+AGG 0.561671 4.1:+66968710 None:intergenic
GTTATCACCGGAAAACCCTC+AGG 0.564479 4.1:-66967496 MS.gene48608:CDS
TTTATCGTTTGAAGCGAAGA+GGG 0.565404 4.1:+66967580 None:intergenic
TTGTTTAGGCGTGGAAGTGG+CGG 0.569287 4.1:-66969993 MS.gene48608:intron
CACCAAGCAAGGGACTTCAA+TGG 0.574912 4.1:-66967635 MS.gene48608:CDS
GGTGTTGGAAGCTTTCTAAG+TGG 0.575161 4.1:-66967190 MS.gene48608:CDS
TTTATGCCCACCTTGTCCAA+TGG 0.578469 4.1:+66968643 None:intergenic
CCAAATGAACTAAGAAGCAT+GGG 0.579849 4.1:-66967238 MS.gene48608:CDS
ACCTTGTGTTCAAGCTTTCG+GGG 0.582205 4.1:-66968621 MS.gene48608:CDS
TGAAAGCACCTAAGAGAGGA+AGG 0.583782 4.1:+66969852 None:intergenic
CTCAGGAGAACAATATGTAA+GGG 0.583841 4.1:+66968706 None:intergenic
AAGAAGCAAAGGCTGTACTA+AGG 0.584489 4.1:-66967723 MS.gene48608:CDS
TATCTAGTTGCCATTGGACA+AGG 0.587068 4.1:-66968653 MS.gene48608:CDS
AGCACCTAAGAGAGGAAGGA+GGG 0.587650 4.1:+66969856 None:intergenic
TATCATATTATGGAATTCAA+GGG 0.587902 4.1:-66969964 MS.gene48608:CDS
AACTTGTAGCCTTAGTGAGG+TGG 0.589145 4.1:-66967746 MS.gene48608:CDS
CCAGATTCGGACCTAACTGC+GGG 0.591748 4.1:+66970468 None:intergenic
TAATACAATTTACAGGGACT+AGG 0.592138 4.1:-66968758 MS.gene48608:intron
GCAATGATCATTACGACACA+AGG 0.592495 4.1:+66968443 None:intergenic
TAGTGAGGTGGAAGAAGCAA+AGG 0.594771 4.1:-66967734 MS.gene48608:CDS
GCGAAATAGCTTATCAACAA+CGG 0.599644 4.1:-66968309 MS.gene48608:CDS
TGAAGCATTGTGATGCCTGA+GGG 0.600479 4.1:+66967481 None:intergenic
CATTGGTAGCTTCCTCAACA+AGG 0.603095 4.1:-66967803 MS.gene48608:intron
TTACATTCTTCACCAAGCAA+GGG 0.604573 4.1:-66967645 MS.gene48608:CDS
GAGAACAATATGTAAGGGAA+GGG 0.609361 4.1:+66968711 None:intergenic
GTTTCTTGCTGGATTAAGTG+TGG 0.609537 4.1:-66967068 MS.gene48608:CDS
TTGAAGCATTGTGATGCCTG+AGG 0.610945 4.1:+66967480 None:intergenic
GCAATGAAAGCACCTAAGAG+AGG 0.613237 4.1:+66969848 None:intergenic
CCGGTGATAACTCTTGCTAG+TGG 0.615685 4.1:+66967508 None:intergenic
CTAGTTGCCATTGGACAAGG+TGG 0.620501 4.1:-66968650 MS.gene48608:CDS
ACCAGATTCGGACCTAACTG+CGG 0.636959 4.1:+66970467 None:intergenic
CCACTAGCAAGAGTTATCAC+CGG 0.639006 4.1:-66967508 MS.gene48608:CDS
ATCGTTTGAAGCGAAGAGGG+AGG 0.642861 4.1:+66967583 None:intergenic
AAGCACCTAAGAGAGGAAGG+AGG 0.649038 4.1:+66969855 None:intergenic
TGAAACTTGTAGCCTTAGTG+AGG 0.657268 4.1:-66967749 MS.gene48608:CDS
TAGTTGCCATTGGACAAGGT+GGG 0.664534 4.1:-66968649 MS.gene48608:CDS
GTGTTAGCAATGCCTTGTTG+AGG 0.670247 4.1:+66967791 None:intergenic
TAGGCGTGGAAGTGGCGGAG+AGG 0.670501 4.1:-66969988 MS.gene48608:CDS
TCATACATTTATAATCACAA+AGG 0.673451 4.1:-66967013 MS.gene48608:CDS
ACTTGATATCATATCTAACA+GGG 0.676492 4.1:-66969940 MS.gene48608:CDS
GGGCTGTCCTCTGTAATCAA+CGG 0.683414 4.1:+66970488 None:intergenic
CAATGATCATTACGACACAA+GGG 0.688557 4.1:+66968444 None:intergenic
TGTACCTATGATAAACCAAG+CGG 0.704715 4.1:+66970434 None:intergenic
CTGTTCCAATGAGTATATGG+TGG 0.715883 4.1:-66967342 MS.gene48608:CDS
GATTTGGAAGAAGAATGTGA+AGG 0.730655 4.1:-66968287 MS.gene48608:CDS
GATGGTGCCGTTGATTACAG+AGG 0.758127 4.1:-66970495 MS.gene48608:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATTTATTTTTTAAA+TGG - chr4.1:66967497-66967516 MS.gene48608:CDS 0.0%
!!! AAATGATTCTAAAATAATTT+TGG + chr4.1:66968325-66968344 None:intergenic 10.0%
!!! AATTTTTATATCAATATTCA+TGG - chr4.1:66970100-66970119 MS.gene48608:intron 10.0%
!!! TGTTTTTATCATAATAATTA+AGG - chr4.1:66967149-66967168 MS.gene48608:CDS 10.0%
!! AAAGAGATATAAAATTGATT+TGG + chr4.1:66967457-66967476 None:intergenic 15.0%
!! TGTATGATATATTACAAAAA+TGG - chr4.1:66968714-66968733 MS.gene48608:CDS 15.0%
!! TTTATCATAATAATTAAGGT+TGG - chr4.1:66967153-66967172 MS.gene48608:CDS 15.0%
!!! AAAGAATTAATTTTGTCTAA+TGG + chr4.1:66967983-66968002 None:intergenic 15.0%
!!! ACCAAAATAAAATTTTGTAA+CGG + chr4.1:66967932-66967951 None:intergenic 15.0%
!! ATATACAAGATAACATTAGT+TGG - chr4.1:66969059-66969078 MS.gene48608:intron 20.0%
!! TAAAGTAATTAACATTTGGA+AGG + chr4.1:66969430-66969449 None:intergenic 20.0%
!! TATACAAGATAACATTAGTT+GGG - chr4.1:66969060-66969079 MS.gene48608:intron 20.0%
!! TATCATATTATGGAATTCAA+GGG - chr4.1:66967579-66967598 MS.gene48608:CDS 20.0%
!! TCATACATTTATAATCACAA+AGG - chr4.1:66970530-66970549 MS.gene48608:CDS 20.0%
!! TGTTATGATCACAATATAAA+AGG + chr4.1:66969624-66969643 None:intergenic 20.0%
!!! ACCGTTACAAAATTTTATTT+TGG - chr4.1:66967928-66967947 MS.gene48608:intron 20.0%
!!! CACATTTTGATTACTTTTAT+TGG - chr4.1:66970453-66970472 MS.gene48608:CDS 20.0%
!!! CTTTCAGTGTTTTAATTTAT+TGG + chr4.1:66969680-66969699 None:intergenic 20.0%
!!! GAATTATGAATGTTTTGTTT+AGG - chr4.1:66967536-66967555 MS.gene48608:CDS 20.0%
!!! TCTTTGATAGAATATTTTAG+CGG - chr4.1:66970386-66970405 MS.gene48608:intron 20.0%
!!! TTCATTTTAGGAGAAATTTT+TGG - chr4.1:66967878-66967897 MS.gene48608:intron 20.0%
!!! TTTTTACACTCTAAAATGAA+TGG - chr4.1:66967814-66967833 MS.gene48608:intron 20.0%
! AACTTGATATCATATCTAAC+AGG - chr4.1:66967602-66967621 MS.gene48608:CDS 25.0%
! AATTTGATTACAATACGTGT+TGG - chr4.1:66969595-66969614 MS.gene48608:intron 25.0%
! ACTTGATATCATATCTAACA+GGG - chr4.1:66967603-66967622 MS.gene48608:CDS 25.0%
! AGAGAGAAAGATTGATTATT+TGG - chr4.1:66969572-66969591 MS.gene48608:intron 25.0%
! CATTATTAATGTTTCATCCT+AGG - chr4.1:66969526-66969545 MS.gene48608:intron 25.0%
! CTAAAATGAACCATTAACTA+TGG + chr4.1:66967868-66967887 None:intergenic 25.0%
! GACATATAGGTTTAACATTA+AGG - chr4.1:66969150-66969169 MS.gene48608:intron 25.0%
! GTATCATATTATGGAATTCA+AGG - chr4.1:66967578-66967597 MS.gene48608:CDS 25.0%
! GTTAGGATATGTAGAATAAA+GGG + chr4.1:66968646-66968665 None:intergenic 25.0%
! TGCTTCTCTTATCTATATAT+TGG - chr4.1:66967754-66967773 MS.gene48608:CDS 25.0%
! TGTTAGGATATGTAGAATAA+AGG + chr4.1:66968647-66968666 None:intergenic 25.0%
! TGTTATATATGTTAAACGTC+CGG + chr4.1:66968153-66968172 None:intergenic 25.0%
! TTGCAGAAATAAATTCCAAA+TGG + chr4.1:66968550-66968569 None:intergenic 25.0%
! TTGGCTTAATACAATTTACA+GGG - chr4.1:66968779-66968798 MS.gene48608:intron 25.0%
!! AATTTTTACGCCATAGTTAA+TGG - chr4.1:66967855-66967874 MS.gene48608:intron 25.0%
!! ATTGGCTTAATACAATTTAC+AGG - chr4.1:66968778-66968797 MS.gene48608:intron 25.0%
!! CATAGTTAATGGTTCATTTT+AGG - chr4.1:66967866-66967885 MS.gene48608:intron 25.0%
!! TTATTGGAATTAGTTTGAGT+TGG + chr4.1:66969664-66969683 None:intergenic 25.0%
!!! ATATAGAGACACTTTTAGTT+TGG - chr4.1:66968249-66968268 MS.gene48608:intron 25.0%
!!! GAGATATAAAATTGATTTGG+TGG + chr4.1:66967454-66967473 None:intergenic 25.0%
!!! GCTCTAAAGTAATTAACATT+TGG + chr4.1:66969434-66969453 None:intergenic 25.0%
!!! GCTTTGATTGTTTTTTTACT+TGG - chr4.1:66969121-66969140 MS.gene48608:intron 25.0%
!!! TTAGCTTGTAATTTCTACAT+TGG - chr4.1:66969723-66969742 MS.gene48608:intron 25.0%
!!! TTGTTTTTTTACTTGGAACA+AGG - chr4.1:66969128-66969147 MS.gene48608:intron 25.0%
!!! TTTTAAATGGTGATGAGAAT+TGG - chr4.1:66967510-66967529 MS.gene48608:CDS 25.0%
AAGAATGTGAATATTTGGTC+TGG - chr4.1:66967656-66967675 MS.gene48608:CDS 30.0%
AAGAGTCCCTTTATTAGAAT+TGG - chr4.1:66969181-66969200 MS.gene48608:intron 30.0%
AATAAGATTACTTGTGACTC+AGG + chr4.1:66968857-66968876 None:intergenic 30.0%
AATACTGCAAATACTTGAAC+AGG + chr4.1:66968595-66968614 None:intergenic 30.0%
ACACGACCAATTCTAATAAA+GGG + chr4.1:66969190-66969209 None:intergenic 30.0%
AGAAAGATTAGCAAAAACCA+TGG + chr4.1:66968677-66968696 None:intergenic 30.0%
CAGAACTTTCTATGTAATTC+TGG + chr4.1:66968196-66968215 None:intergenic 30.0%
CATAGTCAAACCAAAACTAA+AGG + chr4.1:66967293-66967312 None:intergenic 30.0%
CTACAAGTTTCATCTTCTTT+GGG + chr4.1:66969786-66969805 None:intergenic 30.0%
CTAGAAGCTCATTCTTTAAT+TGG - chr4.1:66968984-66969003 MS.gene48608:intron 30.0%
CTGCTAAGAATGTGAATATT+TGG - chr4.1:66967651-66967670 MS.gene48608:CDS 30.0%
TAATACAATTTACAGGGACT+AGG - chr4.1:66968785-66968804 MS.gene48608:intron 30.0%
TACACGACCAATTCTAATAA+AGG + chr4.1:66969191-66969210 None:intergenic 30.0%
TTCTGACGCTAATAATACTT+TGG + chr4.1:66967335-66967354 None:intergenic 30.0%
! CTAGTTTGCATCTAAACTAA+TGG - chr4.1:66968488-66968507 MS.gene48608:CDS 30.0%
! TAGTCTTTTCATCTCAACAA+AGG + chr4.1:66970138-66970157 None:intergenic 30.0%
! TATTTGAGTATTGTTGGTGT+TGG - chr4.1:66970338-66970357 MS.gene48608:intron 30.0%
! TGGTATTTTACTATGATTGC+AGG - chr4.1:66969007-66969026 MS.gene48608:intron 30.0%
! TTTTCGCAGCTATAAGAAAT+TGG - chr4.1:66969212-66969231 MS.gene48608:intron 30.0%
!! AATCTGAAAGTATCTTGAAG+GGG - chr4.1:66968442-66968461 MS.gene48608:CDS 30.0%
!! ACAATCTGAAAGTATCTTGA+AGG - chr4.1:66968440-66968459 MS.gene48608:CDS 30.0%
!! ACTCCAAACAAAAAGTATTG+AGG + chr4.1:66970233-66970252 None:intergenic 30.0%
!! ACTCTTTGAACCTTTAGTTT+TGG - chr4.1:66967280-66967299 MS.gene48608:CDS 30.0%
!! CAATCTGAAAGTATCTTGAA+GGG - chr4.1:66968441-66968460 MS.gene48608:CDS 30.0%
!! GGCGTAAAAATTTAAGTTGT+TGG + chr4.1:66967847-66967866 None:intergenic 30.0%
!! GTTCCTCAATACTTTTTGTT+TGG - chr4.1:66970227-66970246 MS.gene48608:intron 30.0%
!! TATAAAATTGATTTGGTGGC+CGG + chr4.1:66967450-66967469 None:intergenic 30.0%
!!! AGGAGAAATTTTTGGTATCA+TGG - chr4.1:66967886-66967905 MS.gene48608:intron 30.0%
!!! AGTTTCTGAGGTTTTTACTA+TGG - chr4.1:66970250-66970269 MS.gene48608:intron 30.0%
!!! ATTTTTGGTATCATGGAGTT+TGG - chr4.1:66967893-66967912 MS.gene48608:intron 30.0%
!!! TACTTTTATTGGTTTCTTGC+TGG - chr4.1:66970464-66970483 MS.gene48608:CDS 30.0%
!!! TTCATTGCTGATTCTTTTCT+TGG - chr4.1:66967710-66967729 MS.gene48608:CDS 30.0%
AAACCTTGTGTTCAAGCTTT+CGG - chr4.1:66968920-66968939 MS.gene48608:intron 35.0%
ACTAGTATGAGATCTTGTCT+TGG + chr4.1:66968621-66968640 None:intergenic 35.0%
ACTCAGGAGAACAATATGTA+AGG + chr4.1:66968841-66968860 None:intergenic 35.0%
AGATGCAAACTAGATGCAAA+TGG + chr4.1:66968481-66968500 None:intergenic 35.0%
AGGAATATGTGCTCATAGAT+AGG + chr4.1:66970014-66970033 None:intergenic 35.0%
ATCACAATGCTTCAACGAAT+TGG - chr4.1:66970071-66970090 MS.gene48608:intron 35.0%
ATGGAGAGAACAATATTTCC+TGG - chr4.1:66969927-66969946 MS.gene48608:CDS 35.0%
ATTAGTTGGGTTCTTGGATT+TGG - chr4.1:66969073-66969092 MS.gene48608:intron 35.0%
CAACTGTTCCAATGAGTATA+TGG - chr4.1:66970198-66970217 MS.gene48608:intron 35.0%
CAATGATCATTACGACACAA+GGG + chr4.1:66969102-66969121 None:intergenic 35.0%
CCATGCTTCTTAGTTCATTT+GGG + chr4.1:66970307-66970326 None:intergenic 35.0%
CTAAAAATAGCACAAGAGCA+TGG - chr4.1:66970155-66970174 MS.gene48608:intron 35.0%
CTAGTATGAGATCTTGTCTT+GGG + chr4.1:66968620-66968639 None:intergenic 35.0%
CTCAGGAGAACAATATGTAA+GGG + chr4.1:66968840-66968859 None:intergenic 35.0%
CTTCGCTTCAAACGATAAAA+TGG - chr4.1:66969967-66969986 MS.gene48608:CDS 35.0%
GAAGCTCATTCTTTAATTGG+TGG - chr4.1:66968987-66969006 MS.gene48608:intron 35.0%
GACGATGAAGAATATGATGA+TGG + chr4.1:66968573-66968592 None:intergenic 35.0%
GAGAACAATATGTAAGGGAA+GGG + chr4.1:66968835-66968854 None:intergenic 35.0%
GATAACATTAGTTGGGTTCT+TGG - chr4.1:66969067-66969086 MS.gene48608:intron 35.0%
GATGCAAACTAGATGCAAAT+GGG + chr4.1:66968480-66968499 None:intergenic 35.0%
GATTTGGAAGAAGAATGTGA+AGG - chr4.1:66969256-66969275 MS.gene48608:intron 35.0%
GCGAAATAGCTTATCAACAA+CGG - chr4.1:66969234-66969253 MS.gene48608:intron 35.0%
GCTACAAGTTTCATCTTCTT+TGG + chr4.1:66969787-66969806 None:intergenic 35.0%
GCTCAATTAGCTATTGTTGA+TGG - chr4.1:66967030-66967049 MS.gene48608:CDS 35.0%
GGAATATGTGCTCATAGATA+GGG + chr4.1:66970013-66970032 None:intergenic 35.0%
TACTTGGAACAAGGACATAT+AGG - chr4.1:66969137-66969156 MS.gene48608:intron 35.0%
TGTACCTATGATAAACCAAG+CGG + chr4.1:66967112-66967131 None:intergenic 35.0%
TTACATTCTTCACCAAGCAA+GGG - chr4.1:66969898-66969917 MS.gene48608:CDS 35.0%
TTTACATTCTTCACCAAGCA+AGG - chr4.1:66969897-66969916 MS.gene48608:CDS 35.0%
! ACTAAGGTTGGTTCCAATTT+GGG - chr4.1:66969836-66969855 MS.gene48608:CDS 35.0%
! CCAAATGAACTAAGAAGCAT+GGG - chr4.1:66970305-66970324 MS.gene48608:intron 35.0%
! GCACTCTATTTGAGTATTGT+TGG - chr4.1:66970332-66970351 MS.gene48608:intron 35.0%
! TACTAAGGTTGGTTCCAATT+TGG - chr4.1:66969835-66969854 MS.gene48608:CDS 35.0%
! TAGCTTATCAACAACGGATT+TGG - chr4.1:66969240-66969259 MS.gene48608:intron 35.0%
! TTTAGAGCTCAGTTAGTGTT+TGG - chr4.1:66969446-66969465 MS.gene48608:intron 35.0%
! TTTATCGTTTGAAGCGAAGA+GGG + chr4.1:66969966-66969985 None:intergenic 35.0%
!! ATAAAATTGATTTGGTGGCC+GGG + chr4.1:66967449-66967468 None:intergenic 35.0%
!! CTTTTTGTTTGGAGTTTCTG+AGG - chr4.1:66970238-66970257 MS.gene48608:intron 35.0%
!! GGAATTAGTTTGAGTTGGTT+AGG + chr4.1:66969659-66969678 None:intergenic 35.0%
!! TCTGAGGTTTTTACTATGGT+TGG - chr4.1:66970254-66970273 MS.gene48608:intron 35.0%
!! TTTTATCGTTTGAAGCGAAG+AGG + chr4.1:66969967-66969986 None:intergenic 35.0%
!!! ATGAATGTTTTGTTTAGGCG+TGG - chr4.1:66967541-66967560 MS.gene48608:CDS 35.0%
!!! CATGTTTTGATGTTGGTCAT+TGG - chr4.1:66968760-66968779 MS.gene48608:intron 35.0%
!!! TAGAGACACTTTTAGTTTGG+TGG - chr4.1:66968252-66968271 MS.gene48608:intron 35.0%
!!! TTTTACTATGGTTGGTCTTC+AGG - chr4.1:66970262-66970281 MS.gene48608:intron 35.0%
AAACCATGGAAGACAGTGTT+AGG + chr4.1:66968663-66968682 None:intergenic 40.0%
AACCTTGTGTTCAAGCTTTC+GGG - chr4.1:66968921-66968940 MS.gene48608:intron 40.0%
AAGAAGCAAAGGCTGTACTA+AGG - chr4.1:66969820-66969839 MS.gene48608:CDS 40.0%
ACCAAAGTTGAAGCCCAAAT+TGG + chr4.1:66969852-66969871 None:intergenic 40.0%
ACCAACCACCATATACTCAT+TGG + chr4.1:66970209-66970228 None:intergenic 40.0%
AGATCCGCTTGGTTTATCAT+AGG - chr4.1:66967105-66967124 MS.gene48608:CDS 40.0%
AGTACTAGATTCAAACCCCA+AGG + chr4.1:66968078-66968097 None:intergenic 40.0%
ATCCTAGGTAGAGATCAACA+AGG - chr4.1:66969541-66969560 MS.gene48608:intron 40.0%
CCCAAATGAACTAAGAAGCA+TGG - chr4.1:66970304-66970323 MS.gene48608:intron 40.0%
CCCATGCTTCTTAGTTCATT+TGG + chr4.1:66970308-66970327 None:intergenic 40.0%
CTCATTGGAACAGTTGCATT+TGG + chr4.1:66970194-66970213 None:intergenic 40.0%
CTGTTCCAATGAGTATATGG+TGG - chr4.1:66970201-66970220 MS.gene48608:intron 40.0%
GCAAAGATGATCGTGAAAGT+TGG - chr4.1:66970408-66970427 MS.gene48608:intron 40.0%
GCAATGATCATTACGACACA+AGG + chr4.1:66969103-66969122 None:intergenic 40.0%
GGAGAACAATATGTAAGGGA+AGG + chr4.1:66968836-66968855 None:intergenic 40.0%
GGATCTCCAATAACCAGATT+CGG + chr4.1:66967091-66967110 None:intergenic 40.0%
GTTTCTTGCTGGATTAAGTG+TGG - chr4.1:66970475-66970494 MS.gene48608:CDS 40.0%
TATCCTAACACTGTCTTCCA+TGG - chr4.1:66968657-66968676 MS.gene48608:CDS 40.0%
TATCTAGTTGCCATTGGACA+AGG - chr4.1:66968890-66968909 MS.gene48608:intron 40.0%
TCACTCTATCTAGTTGCCAT+TGG - chr4.1:66968884-66968903 MS.gene48608:intron 40.0%
TCATCTTCTTTGGGTCCAAT+TGG + chr4.1:66969777-66969796 None:intergenic 40.0%
TCCAATGAGTATATGGTGGT+TGG - chr4.1:66970205-66970224 MS.gene48608:intron 40.0%
TCCAATTTGGGCTTCAACTT+TGG - chr4.1:66969848-66969867 MS.gene48608:CDS 40.0%
TTAGGTCCGAATCTGGTTAT+TGG - chr4.1:66967082-66967101 MS.gene48608:CDS 40.0%
TTCCTTGTTGATCTCTACCT+AGG + chr4.1:66969546-66969565 None:intergenic 40.0%
TTGTGTCTTCACACACCATT+TGG - chr4.1:66968532-66968551 MS.gene48608:CDS 40.0%
! TGAAACTTGTAGCCTTAGTG+AGG - chr4.1:66969794-66969813 MS.gene48608:CDS 40.0%
! TGGGCTTCAACTTTGGTTTA+TGG - chr4.1:66969855-66969874 MS.gene48608:CDS 40.0%
!! AAGGCATTGCTAACACCAAT+TGG - chr4.1:66969759-66969778 MS.gene48608:intron 40.0%
!! AGGTTGGTTTGATAGAGAGT+TGG - chr4.1:66967169-66967188 MS.gene48608:CDS 40.0%
!!! GTGTGCTCATGTTTTGATGT+TGG - chr4.1:66968753-66968772 MS.gene48608:intron 40.0%
AAACGTCCGGAATTTGCACT+GGG + chr4.1:66968140-66968159 None:intergenic 45.0%
ACCTTGTGTTCAAGCTTTCG+GGG - chr4.1:66968922-66968941 MS.gene48608:intron 45.0%
AGCAAAGGCTGTACTAAGGT+TGG - chr4.1:66969824-66969843 MS.gene48608:CDS 45.0%
AGCTTCTAACAGCACTTCGT+TGG + chr4.1:66967013-66967032 None:intergenic 45.0%
ATGCTTCAACGAATTGGCAC+TGG - chr4.1:66970077-66970096 MS.gene48608:intron 45.0%
CCACTAGCAAGAGTTATCAC+CGG - chr4.1:66970035-66970054 MS.gene48608:intron 45.0%
CTCCTTAACCTTTCTCATCC+CGG - chr4.1:66967428-66967447 MS.gene48608:CDS 45.0%
CTTGCTGGATTAAGTGTGGT+GGG - chr4.1:66970479-66970498 MS.gene48608:CDS 45.0%
TAAACGTCCGGAATTTGCAC+TGG + chr4.1:66968141-66968160 None:intergenic 45.0%
TAGTGAGGTGGAAGAAGCAA+AGG - chr4.1:66969809-66969828 MS.gene48608:CDS 45.0%
TAGTTGCCATTGGACAAGGT+GGG - chr4.1:66968894-66968913 MS.gene48608:intron 45.0%
TCTTGCTGGATTAAGTGTGG+TGG - chr4.1:66970478-66970497 MS.gene48608:CDS 45.0%
TGATAACTCTTGCTAGTGGC+AGG + chr4.1:66970034-66970053 None:intergenic 45.0%
TTGCTTCTTCCACCTCACTA+AGG + chr4.1:66969809-66969828 None:intergenic 45.0%
TTTATGCCCACCTTGTCCAA+TGG + chr4.1:66968903-66968922 None:intergenic 45.0%
! AACTTGTAGCCTTAGTGAGG+TGG - chr4.1:66969797-66969816 MS.gene48608:CDS 45.0%
! CATTGGTAGCTTCCTCAACA+AGG - chr4.1:66969740-66969759 MS.gene48608:intron 45.0%
! GGTGTTGGAAGCTTTCTAAG+TGG - chr4.1:66970353-66970372 MS.gene48608:intron 45.0%
! GTGTTAGCAATGCCTTGTTG+AGG + chr4.1:66969755-66969774 None:intergenic 45.0%
!! CTTCTAGCTCTGTGCTCTTT+AGG + chr4.1:66968971-66968990 None:intergenic 45.0%
!! GCAATGAAAGCACCTAAGAG+AGG + chr4.1:66967698-66967717 None:intergenic 45.0%
!! GCGGAGAGGGTATCATATTA+TGG - chr4.1:66967569-66967588 MS.gene48608:CDS 45.0%
!! TGAAAGCACCTAAGAGAGGA+AGG + chr4.1:66967694-66967713 None:intergenic 45.0%
!! TGAAGCATTGTGATGCCTGA+GGG + chr4.1:66970065-66970084 None:intergenic 45.0%
!! TTGAAGCATTGTGATGCCTG+AGG + chr4.1:66970066-66970085 None:intergenic 45.0%
!!! GTTTTGTTTAGGCGTGGAAG+TGG - chr4.1:66967547-66967566 MS.gene48608:CDS 45.0%
AAGCACCTAAGAGAGGAAGG+AGG + chr4.1:66967691-66967710 None:intergenic 50.0%
AATCTAGTACTCCGAGTCCG+AGG - chr4.1:66968087-66968106 MS.gene48608:intron 50.0%
ACCAGATTCGGACCTAACTG+CGG + chr4.1:66967079-66967098 None:intergenic 50.0%
AGCACCTAAGAGAGGAAGGA+GGG + chr4.1:66967690-66967709 None:intergenic 50.0%
AGTTGCCAGTTGCATCCTTG+GGG - chr4.1:66968060-66968079 MS.gene48608:intron 50.0%
ATGTCGCCCAGTGCAAATTC+CGG - chr4.1:66968131-66968150 MS.gene48608:intron 50.0%
CACCAAGCAAGGGACTTCAA+TGG - chr4.1:66969908-66969927 MS.gene48608:CDS 50.0%
CAGTTGCCAGTTGCATCCTT+GGG - chr4.1:66968059-66968078 MS.gene48608:intron 50.0%
CCGGTGATAACTCTTGCTAG+TGG + chr4.1:66970038-66970057 None:intergenic 50.0%
CGTTAGCAACGTCTTAGCAC+CGG + chr4.1:66967228-66967247 None:intergenic 50.0%
CTAGTTGCCATTGGACAAGG+TGG - chr4.1:66968893-66968912 MS.gene48608:intron 50.0%
CTCCATTGAAGTCCCTTGCT+TGG + chr4.1:66969913-66969932 None:intergenic 50.0%
CTGGTTATTGGAGATCCGCT+TGG - chr4.1:66967094-66967113 MS.gene48608:CDS 50.0%
GCGGATTCAACTTCTGCTAC+CGG - chr4.1:66967206-66967225 MS.gene48608:CDS 50.0%
GGGCTGTCCTCTGTAATCAA+CGG + chr4.1:66967058-66967077 None:intergenic 50.0%
GTTATCACCGGAAAACCCTC+AGG - chr4.1:66970047-66970066 MS.gene48608:intron 50.0%
GTTGCAGTGCTCTGTTTGAG+TGG + chr4.1:66967629-66967648 None:intergenic 50.0%
TCAAACCCCAAGGATGCAAC+TGG + chr4.1:66968068-66968087 None:intergenic 50.0%
TGTCACCATTACCTCGGACT+CGG + chr4.1:66968101-66968120 None:intergenic 50.0%
TTGTTTAGGCGTGGAAGTGG+CGG - chr4.1:66967550-66967569 MS.gene48608:CDS 50.0%
! ATCGTTTGAAGCGAAGAGGG+AGG + chr4.1:66969963-66969982 None:intergenic 50.0%
! GAGGGAGGTATGTCAAATCC+AGG + chr4.1:66969948-66969967 None:intergenic 50.0%
! TGTGATGCCTGAGGGTTTTC+CGG + chr4.1:66970057-66970076 None:intergenic 50.0%
!! GATGGTGCCGTTGATTACAG+AGG - chr4.1:66967048-66967067 MS.gene48608:CDS 50.0%
!! GTCTGGTGTCACCATTACCT+CGG + chr4.1:66968107-66968126 None:intergenic 50.0%
AGAAGTTGAATCCGCGTGCG+GGG + chr4.1:66967201-66967220 None:intergenic 55.0%
CACTGGGCGACATGTTAGTC+TGG + chr4.1:66968124-66968143 None:intergenic 55.0%
CAGAAGTTGAATCCGCGTGC+GGG + chr4.1:66967202-66967221 None:intergenic 55.0%
CCAGATTCGGACCTAACTGC+GGG + chr4.1:66967078-66967097 None:intergenic 55.0%
GCAGAAGTTGAATCCGCGTG+CGG + chr4.1:66967203-66967222 None:intergenic 55.0%
GCAGTTGCCAGTTGCATCCT+TGG - chr4.1:66968058-66968077 MS.gene48608:intron 55.0%
GCCCCGAAAGCTTGAACACA+AGG + chr4.1:66968926-66968945 None:intergenic 55.0%
GCTTCCCTCCTTCCTCTCTT+AGG - chr4.1:66967683-66967702 MS.gene48608:CDS 55.0%
GGCCGGGATGAGAAAGGTTA+AGG + chr4.1:66967433-66967452 None:intergenic 55.0%
GTACTCCGAGTCCGAGGTAA+TGG - chr4.1:66968093-66968112 MS.gene48608:intron 55.0%
! TTTGGTGGCCGGGATGAGAA+AGG + chr4.1:66967439-66967458 None:intergenic 55.0%
ACAGAGGACAGCCCGCAGTT+AGG - chr4.1:66967064-66967083 MS.gene48608:CDS 60.0%
CCCGCAGTTAGGTCCGAATC+TGG - chr4.1:66967075-66967094 MS.gene48608:CDS 60.0%
GTTGGTCAATACCCCGCACG+CGG - chr4.1:66967187-66967206 MS.gene48608:CDS 60.0%
! AGGCGTGGAAGTGGCGGAGA+GGG - chr4.1:66967556-66967575 MS.gene48608:CDS 65.0%
! TAGGCGTGGAAGTGGCGGAG+AGG - chr4.1:66967555-66967574 MS.gene48608:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 66967000 66970565 66967000 ID=MS.gene48608
chr4.1 mRNA 66967000 66970565 66967000 ID=MS.gene48608.t1;Parent=MS.gene48608
chr4.1 exon 66970439 66970565 66970439 ID=MS.gene48608.t1.exon1;Parent=MS.gene48608.t1
chr4.1 CDS 66970439 66970565 66970439 ID=cds.MS.gene48608.t1;Parent=MS.gene48608.t1
chr4.1 exon 66969790 66970007 66969790 ID=MS.gene48608.t1.exon2;Parent=MS.gene48608.t1
chr4.1 CDS 66969790 66970007 66969790 ID=cds.MS.gene48608.t1;Parent=MS.gene48608.t1
chr4.1 exon 66968254 66968765 66968254 ID=MS.gene48608.t1.exon3;Parent=MS.gene48608.t1
chr4.1 CDS 66968254 66968765 66968254 ID=cds.MS.gene48608.t1;Parent=MS.gene48608.t1
chr4.1 exon 66967000 66967816 66967000 ID=MS.gene48608.t1.exon4;Parent=MS.gene48608.t1
chr4.1 CDS 66967000 66967816 66967000 ID=cds.MS.gene48608.t1;Parent=MS.gene48608.t1
Gene Sequence

>MS.gene48608

ATGGCAGAATCCAACGAAGTGCTGTTAGAAGCTCAATTAGCTATTGTTGATGGTGCCGTTGATTACAGAGGACAGCCCGCAGTTAGGTCCGAATCTGGTTATTGGAGATCCGCTTGGTTTATCATAGGTACACATTTTCAGTACTGATTTGTTTTTATCATAATAATTAAGGTTGGTTTGATAGAGAGTTGGTCAATACCCCGCACGCGGATTCAACTTCTGCTACCGGTGCTAAGACGTTGCTAACGAATGATATGTCGCTCTAAATATTAGTGTCAGCACTCTTTGAACCTTTAGTTTTGGTTTGACTATGATGAACTTTCGAAACTCTACCAAAGTATTATTAGCGTCAGAAGAACATTATACACAAGTATTATTAGTATTAGTTGATAAAATCTTAGATAAACTTTTTACTAAAAAAAGCTCTACTCCTTAACCTTTCTCATCCCGGCCACCAAATCAATTTTATATCTCTTTCTGCAGCTCAATTAATTCATTTTTTTATTTATTTTTTAAATGGTGATGAGAATTGGAATGAATTATGAATGTTTTGTTTAGGCGTGGAAGTGGCGGAGAGGGTATCATATTATGGAATTCAAGGGAACTTGATATCATATCTAACAGGGCCACTCAAACAGAGCACTGCAACAGCTGCTAAGAATGTGAATATTTGGTCTGGAACTGCTTCCCTCCTTCCTCTCTTAGGTGCTTTCATTGCTGATTCTTTTCTTGGACGCTATCGCACAATCATACTTGCTTCTCTTATCTATATATTGGTTAGTTCAGTTCGTCTCTTTTATTTTTGTCTCTTCAATTTTTACACTCTAAAATGAATGGCTTCTCTCCAACAACTTAAATTTTTACGCCATAGTTAATGGTTCATTTTAGGAGAAATTTTTGGTATCATGGAGTTTGGATGAGTATCAGTACCGTTACAAAATTTTATTTTGGTGATTTCTACTCATTTTTTTGTTGTGCTACCATTAGACAAAATTAATTCTTTTATTAATTTAGTTTAAAATAATTACTATAAAAAAGTTACATGACATTATATGACAGCAGTTGCCAGTTGCATCCTTGGGGTTTGAATCTAGTACTCCGAGTCCGAGGTAATGGTGACACCAGACTAACATGTCGCCCAGTGCAAATTCCGGACGTTTAACATATATAACATTTCAATTTATTTCAATTTACCAGAATTACATAGAAAGTTCTGTCTCTTAACTAAATGACACAAGTTTCTTTATACATATAGAGACACTTTTAGTTTGGTGGCTGAAACAAAAGACAAAAATCATGATTTCTTGACTCAAAATTATATGCCAAAATTATTTTAGAATCATTTCTTTTATGCTTCGTGCACGAGATTAATTGAGAGAATTTTTTTTCTGTAAAATTCTATTGATCGATACTATTGATTTGAGTGTGCGATTCAAATCAACAATCTGAAAGTATCTTGAAGGGGATTTGCTAGTTACCCATTTGCATCTAGTTTGCATCTAAACTAATGGAGACAAATCAAACTTAAAGCTTTGTGTCTTCACACACCATTTGGAATTTATTTCTGCAACCATCATCATATTCTTCATCGTCCTGTTCAAGTATTTGCAGTATTCTCCCAAGACAAGATCTCATACTAGTTTCCCTTTATTCTACATATCCTAACACTGTCTTCCATGGTTTTTGCTAATCTTTCTATCTTTCATTATTTTGATGTATGATATATTACAAAAATGGATAGACAGATAGTTATGTGTGCTCATGTTTTGATGTTGGTCATTGGCTTAATACAATTTACAGGGACTAGGCTTGTTAACACTATCAGCTTTGCTCCCTTCCCTTACATATTGTTCTCCTGAGTCACAAGTAATCTTATTCTTCGTCTCACTCTATCTAGTTGCCATTGGACAAGGTGGGCATAAACCTTGTGTTCAAGCTTTCGGGGCTGATCAATTCGACGAAAAGCATCCTAAAGAGCACAGAGCTAGAAGCTCATTCTTTAATTGGTGGTATTTTACTATGATTGCAGGTTGCACAGCAACACTTACGATCTTGAACTATATACAAGATAACATTAGTTGGGTTCTTGGATTTGGAATCCCTTGTGTCGTAATGATCATTGCTTTGATTGTTTTTTTACTTGGAACAAGGACATATAGGTTTAACATTAAGGATAATGACAAGAGTCCCTTTATTAGAATTGGTCGTGTATTTTTCGCAGCTATAAGAAATTGGCGAAATAGCTTATCAACAACGGATTTGGAAGAAGAATGTGAAGGACTACTTCGCTGTCAAAGTTCTGAACAATTCAAGTAAGAATAACAATGCTAACTTATTCTTGTTTTTTAATGTTATGTCTATTAAGTTCAATCTTATTAGTAGCATTCTGAAAGAAGAAAATATGATGAATTACATTATAAGCAACTACCTTCCAAATGTTAATTACTTTAGAGCTCAGTTAGTGTTTGGAATTGAATCTTCTGTTAATATGTATTTTCTTGTATTGATTCATCTACAATTATTGTACATTATTAATGTTTCATCCTAGGTAGAGATCAACAAGGAAAAAAATAGAGAGAAAGATTGATTATTTGGAATTTGATTACAATACGTGTTGGATACCTTTTATATTGTGATCATAACAAAACTAACAAATCCTAACCAACTCAAACTAATTCCAATAAATTAAAACACTGAAAGATTATGACTATATTAATTTTCACTTAGCTTGTAATTTCTACATTGGTAGCTTCCTCAACAAGGCATTGCTAACACCAATTGGACCCAAAGAAGATGAAACTTGTAGCCTTAGTGAGGTGGAAGAAGCAAAGGCTGTACTAAGGTTGGTTCCAATTTGGGCTTCAACTTTGGTTTATGGTATTGTCTTTGCACAAGTTTTTACATTCTTCACCAAGCAAGGGACTTCAATGGAGAGAACAATATTTCCTGGATTTGACATACCTCCCTCTTCGCTTCAAACGATAAAATGGCTTGCAATTGTTCTCTTCTGCCCTATCTATGAGCACATATTCCTGCCACTAGCAAGAGTTATCACCGGAAAACCCTCAGGCATCACAATGCTTCAACGAATTGGCACTGGAATTTTTATATCAATATTCATGGTAGTATTTGCAGCCTTTGTTGAGATGAAAAGACTAAAAATAGCACAAGAGCATGGTCTTGTTGATGATCCAAATGCAACTGTTCCAATGAGTATATGGTGGTTGGTTCCTCAATACTTTTTGTTTGGAGTTTCTGAGGTTTTTACTATGGTTGGTCTTCAGGAATTTTTCTATGATCAAGTCCCAAATGAACTAAGAAGCATGGGACTTGCACTCTATTTGAGTATTGTTGGTGTTGGAAGCTTTCTAAGTGGCTTTCTCATTTCTTTGATAGAATATTTTAGCGGCAAAGATGATCGTGAAAGTTGGTTTTGTGACAATATCAATAAAGCACATTTTGATTACTTTTATTGGTTTCTTGCTGGATTAAGTGTGGTGGGATTTACTTTGTTCATTTACTTTGCAAAATCATACATTTATAATCACAAAGGCACTACCACATAA
Protein sequence

>MS.gene48608.t1

MAESNEVLLEAQLAIVDGAVDYRGQPAVRSESGYWRSAWFIIGVEVAERVSYYGIQGNLISYLTGPLKQSTATAAKNVNIWSGTASLLPLLGAFIADSFLGRYRTIILASLIYILGLGLLTLSALLPSLTYCSPESQVILFFVSLYLVAIGQGGHKPCVQAFGADQFDEKHPKEHRARSSFFNWWYFTMIAGCTATLTILNYIQDNISWVLGFGIPCVVMIIALIVFLLGTRTYRFNIKDNDKSPFIRIGRVFFAAIRNWRNSLSTTDLEEECEGLLRCQSSEQFNFLNKALLTPIGPKEDETCSLSEVEEAKAVLRLVPIWASTLVYGIVFAQVFTFFTKQGTSMERTIFPGFDIPPSSLQTIKWLAIVLFCPIYEHIFLPLARVITGKPSGITMLQRIGTGIFISIFMVVFAAFVEMKRLKIAQEHGLVDDPNATVPMSIWWLVPQYFLFGVSEVFTMVGLQEFFYDQVPNELRSMGLALYLSIVGVGSFLSGFLISLIEYFSGKDDRESWFCDNINKAHFDYFYWFLAGLSVVGFTLFIYFAKSYIYNHKGTTT