AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033758

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033758.t1 MTR_7g084750 96.246 293 11 0 1 293 1 293 0.0 590
MS.gene033758.t1 MTR_7g084770 84.983 293 44 0 1 293 1 293 0.0 538
MS.gene033758.t1 MTR_7g084760 83.959 293 47 0 1 293 1 293 0.0 532
MS.gene033758.t1 MTR_6g033055 78.498 293 63 0 1 293 1 293 1.19e-179 497
MS.gene033758.t1 MTR_6g033085 77.816 293 65 0 1 293 1 293 1.36e-179 496
MS.gene033758.t1 MTR_4g128410 52.577 291 125 4 1 288 1 281 8.75e-103 301
MS.gene033758.t1 MTR_2g089140 53.383 266 114 3 32 289 25 288 3.02e-102 301
MS.gene033758.t1 MTR_4g045853 49.310 290 131 4 6 287 7 288 2.23e-101 298
MS.gene033758.t1 MTR_3g084620 50.342 292 133 3 1 291 1 281 1.48e-100 296
MS.gene033758.t1 MTR_4g097700 52.529 257 120 2 32 287 69 324 1.87e-99 295
MS.gene033758.t1 MTR_4g097640 52.529 257 120 2 32 287 32 287 2.37e-99 293
MS.gene033758.t1 MTR_4g097670 52.529 257 120 2 32 287 32 287 2.37e-99 293
MS.gene033758.t1 MTR_3g084630 54.023 261 112 2 32 291 28 281 2.52e-99 293
MS.gene033758.t1 MTR_8g081040 54.864 257 109 3 32 287 31 281 3.81e-98 290
MS.gene033758.t1 MTR_7g093530 49.828 291 130 4 1 287 1 279 1.33e-96 286
MS.gene033758.t1 MTR_4g128580 53.030 264 112 3 32 287 26 285 7.52e-95 282
MS.gene033758.t1 MTR_4g128570 54.475 257 101 4 32 287 31 272 1.35e-93 278
MS.gene033758.t1 MTR_1g105900 51.938 258 122 2 32 287 34 291 2.33e-93 278
MS.gene033758.t1 MTR_1g105495 48.443 289 145 2 3 287 6 294 2.35e-93 278
MS.gene033758.t1 MTR_4g126920 51.341 261 118 3 32 287 25 281 3.67e-92 275
MS.gene033758.t1 MTR_6g088320 48.106 264 131 4 28 287 22 283 4.41e-92 275
MS.gene033758.t1 MTR_8g102220 51.341 261 107 3 32 291 29 270 1.40e-91 273
MS.gene033758.t1 MTR_4g128400 53.200 250 115 2 39 287 35 283 8.35e-91 271
MS.gene033758.t1 MTR_2g038860 50.566 265 118 3 32 287 26 286 1.60e-89 268
MS.gene033758.t1 MTR_2g038705 50.566 265 118 3 32 287 26 286 1.60e-89 268
MS.gene033758.t1 MTR_8g102910 46.429 280 144 4 12 287 2 279 2.45e-89 268
MS.gene033758.t1 MTR_2g089140 54.667 225 94 2 73 289 1 225 5.56e-89 265
MS.gene033758.t1 MTR_8g013055 47.710 262 136 1 26 287 25 285 6.27e-88 264
MS.gene033758.t1 MTR_4g128590 49.421 259 104 4 39 289 38 277 1.09e-83 253
MS.gene033758.t1 MTR_3g089540 48.594 249 115 3 50 293 48 288 1.49e-82 251
MS.gene033758.t1 MTR_5g091730 49.167 240 115 3 51 289 47 280 1.45e-79 243
MS.gene033758.t1 MTR_7g056090 44.170 283 141 6 7 288 16 282 1.51e-77 238
MS.gene033758.t1 MTR_2g049030 40.925 281 155 7 9 288 7 277 1.62e-67 212
MS.gene033758.t1 MTR_2g049130 43.825 251 134 7 29 277 26 271 6.07e-65 205
MS.gene033758.t1 MTR_5g029100 38.290 269 152 6 30 287 40 305 4.91e-62 200
MS.gene033758.t1 MTR_8g064180 38.189 254 146 5 45 288 59 311 3.23e-61 197
MS.gene033758.t1 MTR_1g033420 36.986 292 168 7 10 290 8 294 2.48e-59 192
MS.gene033758.t1 MTR_7g102260 37.500 264 148 7 32 287 37 291 9.57e-57 184
MS.gene033758.t1 MTR_4g057450 34.680 297 166 8 10 287 17 304 1.40e-56 184
MS.gene033758.t1 MTR_8g090100 36.059 269 155 6 30 287 19 281 1.53e-56 185
MS.gene033758.t1 MTR_1g063020 38.290 269 145 8 29 287 37 294 3.02e-56 183
MS.gene033758.t1 MTR_2g095800 36.552 290 161 10 12 287 29 309 3.49e-55 181
MS.gene033758.t1 MTR_5g029100 38.983 236 130 6 63 287 42 274 4.23e-54 178
MS.gene033758.t1 MTR_8g479340 33.333 186 63 3 103 287 10 135 8.99e-26 99.8
MS.gene033758.t1 MTR_8g032640 33.333 174 108 4 54 225 33 200 4.47e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033758.t1 AT5G13870 77.816 293 65 0 1 293 1 293 1.36e-179 497
MS.gene033758.t1 AT5G13870 77.816 293 65 0 1 293 1 293 1.36e-179 497
MS.gene033758.t1 AT5G13870 77.816 293 65 0 1 293 1 293 1.36e-179 497
MS.gene033758.t1 AT2G06850 75.254 295 70 1 1 292 1 295 4.37e-173 480
MS.gene033758.t1 AT2G06850 80.952 231 44 0 62 292 5 235 1.84e-146 410
MS.gene033758.t1 AT3G23730 52.431 288 123 4 10 291 11 290 1.81e-105 309
MS.gene033758.t1 AT5G65730 51.071 280 133 3 10 288 15 291 1.38e-104 306
MS.gene033758.t1 AT4G03210 53.409 264 119 2 32 293 29 290 5.40e-102 300
MS.gene033758.t1 AT4G14130 55.253 257 108 3 39 291 35 288 7.40e-102 300
MS.gene033758.t1 AT4G37800 50.362 276 133 3 13 287 17 289 3.64e-100 295
MS.gene033758.t1 AT5G57550 50.342 292 134 4 1 289 1 284 4.39e-97 287
MS.gene033758.t1 AT5G57530 55.078 256 107 5 39 290 34 285 3.63e-95 282
MS.gene033758.t1 AT4G13090 50.178 281 133 4 11 288 13 289 4.47e-95 283
MS.gene033758.t1 AT1G11545 50.896 279 127 4 15 287 25 299 4.77e-95 283
MS.gene033758.t1 AT5G57560 49.310 290 128 5 5 287 4 281 5.38e-95 282
MS.gene033758.t1 AT5G57540 52.852 263 116 4 32 290 26 284 1.02e-93 279
MS.gene033758.t1 AT4G30270 51.273 275 113 4 14 287 11 265 1.36e-93 278
MS.gene033758.t1 AT4G03210 51.172 256 120 2 40 293 10 262 1.86e-93 277
MS.gene033758.t1 AT4G25810 50.000 282 119 6 12 287 18 283 2.17e-91 273
MS.gene033758.t1 AT4G28850 45.455 286 149 4 11 291 8 291 4.26e-91 272
MS.gene033758.t1 AT3G25050 46.552 290 143 6 3 287 7 289 2.77e-90 270
MS.gene033758.t1 AT5G48070 53.333 240 106 3 49 287 47 281 1.79e-89 268
MS.gene033758.t1 AT2G14620 47.059 289 143 4 1 287 14 294 5.34e-89 267
MS.gene033758.t1 AT2G18800 45.791 297 140 5 9 287 3 296 1.08e-88 267
MS.gene033758.t1 AT4G25820 51.575 254 115 3 39 288 37 286 8.53e-88 264
MS.gene033758.t1 AT4G13080 44.599 287 154 4 2 288 11 292 2.81e-87 263
MS.gene033758.t1 AT4G30290 52.083 240 109 3 49 287 42 276 5.92e-83 251
MS.gene033758.t1 AT2G14620 52.423 227 106 2 63 287 18 244 3.44e-82 248
MS.gene033758.t1 AT1G65310 53.540 226 99 3 63 287 83 303 6.89e-82 249
MS.gene033758.t1 AT1G65310 53.540 226 99 3 63 287 61 281 7.52e-82 248
MS.gene033758.t1 AT4G30280 52.655 226 101 3 63 287 61 281 1.51e-81 248
MS.gene033758.t1 AT2G14620 52.968 219 101 2 71 287 15 233 2.16e-80 243
MS.gene033758.t1 AT3G44990 39.073 302 160 7 1 287 1 293 1.17e-63 202
MS.gene033758.t1 AT1G32170 35.640 289 174 5 6 287 10 293 9.64e-60 194
MS.gene033758.t1 AT1G14720 34.247 292 185 4 1 287 1 290 2.45e-57 187
MS.gene033758.t1 AT3G48580 38.372 258 143 5 30 287 32 273 5.14e-56 182
MS.gene033758.t1 AT1G10550 37.154 253 140 6 51 287 60 309 1.64e-55 182
MS.gene033758.t1 AT2G36870 39.431 246 134 5 52 289 61 299 1.29e-54 179
MS.gene033758.t1 AT2G01850 34.386 285 178 5 10 287 8 290 3.70e-54 179
MS.gene033758.t1 AT4G18990 36.508 252 141 4 54 287 62 312 1.64e-53 178
MS.gene033758.t1 AT3G48580 38.496 226 124 4 62 287 4 214 1.79e-49 163
MS.gene033758.t1 AT4G18990 32.328 232 130 5 81 287 9 238 1.02e-40 143
MS.gene033758.t1 AT3G48580 32.946 258 137 6 30 287 32 253 1.99e-40 141
MS.gene033758.t1 AT2G36870 44.068 118 58 2 52 161 61 178 7.26e-28 106

Find 68 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCATCATGTTCCGCATTT+TGG 0.142294 7.4:+22379635 None:intergenic
TGAAGGTATACAACAGTTTA+TGG 0.206946 7.4:-22378782 MS.gene033758:CDS
ACATTTGTTTGTAAAATATA+AGG 0.260741 7.4:+22379577 None:intergenic
TTCTTTGTTCTCTATTACCT+TGG 0.292434 7.4:+22379542 None:intergenic
ATCAGTACAGTAATTATAAA+TGG 0.326064 7.4:+22378539 None:intergenic
GTACATCTACCGGCCTTTGC+GGG 0.345454 7.4:+22380169 None:intergenic
GTGCAAAACAAGGTAGAAAA+TGG 0.352762 7.4:-22378635 MS.gene033758:CDS
ACCATAGATACTCAGTTCTA+TGG 0.355438 7.4:-22379489 MS.gene033758:CDS
TTGTGGACAATATTCCAATC+AGG 0.363427 7.4:-22378857 MS.gene033758:CDS
TACAAAAGTTTCCACATTGA+TGG 0.366093 7.4:-22378687 MS.gene033758:CDS
AAACTGTTGTATACCTTCAT+TGG 0.366710 7.4:+22378786 None:intergenic
ACCTTCATTGGTTGATCAAA+TGG 0.372148 7.4:+22378798 None:intergenic
TCTAAAGGATCATACTTGTT+TGG 0.386128 7.4:-22379896 MS.gene033758:CDS
CTCAATGGAAAAGGATAAAG+TGG 0.398385 7.4:-22378578 MS.gene033758:CDS
GTTTATGGAATGCAGATGAT+TGG 0.401396 7.4:-22378767 MS.gene033758:CDS
ACAAGTATGATCCTTTAGAT+TGG 0.403137 7.4:+22379900 None:intergenic
TCCATAGAACTGAGTATCTA+TGG 0.413646 7.4:+22379488 None:intergenic
AGGCCGGTAGATGTACCTTT+TGG 0.417023 7.4:-22380162 MS.gene033758:CDS
TCCATTTGATCAACCAATGA+AGG 0.419877 7.4:-22378799 MS.gene033758:CDS
GAGAACAAAGAATATTTCTC+TGG 0.421708 7.4:-22379531 MS.gene033758:CDS
TCACGGTCTCTTCTACATTC+AGG 0.438875 7.4:+22378492 None:intergenic
GGTACATCTACCGGCCTTTG+CGG 0.443496 7.4:+22380168 None:intergenic
CACATCAAATACTTTAATGA+AGG 0.454409 7.4:-22380108 MS.gene033758:CDS
ATGAACATAAAGATGGTTCC+TGG 0.454798 7.4:-22379863 MS.gene033758:CDS
TTACAAACAAATGTGTTCAC+TGG 0.460974 7.4:-22379568 MS.gene033758:CDS
GTGACCTTGATGCTGCTCAA+TGG 0.469367 7.4:-22378593 MS.gene033758:CDS
TTTATGGAATGCAGATGATT+GGG 0.473254 7.4:-22378766 MS.gene033758:CDS
TTGATGCTGCTCAATGGAAA+AGG 0.491805 7.4:-22378587 MS.gene033758:CDS
CAGCGCCACCCCGCAAAGGC+CGG 0.496418 7.4:-22380178 MS.gene033758:CDS
GGTACTGGCTTCCAATCTAA+AGG 0.501003 7.4:-22379911 MS.gene033758:CDS
ATGTATGTGTAGGTTCTTTG+TGG 0.502520 7.4:-22378874 MS.gene033758:intron
ATGGTTCCTGGTGATTCAGC+TGG 0.504587 7.4:-22379851 MS.gene033758:CDS
GGTACATAGTTTCGACCAAA+AGG 0.510742 7.4:+22380147 None:intergenic
CTTTCAGCGCCACCCCGCAA+AGG 0.527079 7.4:-22380182 MS.gene033758:CDS
GTCGAAACTATGTACCCACT+TGG 0.530233 7.4:-22380140 MS.gene033758:CDS
TCTTTATGTTCATGCTGAAG+TGG 0.533369 7.4:+22379873 None:intergenic
CTACATTCAGGAGGTATCTG+AGG 0.537443 7.4:+22378504 None:intergenic
CGACCAAAAGGTACATCTAC+CGG 0.539669 7.4:+22380159 None:intergenic
TGCAGATGATTGGGCAACAA+GGG 0.548054 7.4:-22378757 MS.gene033758:CDS
ATGCAGATGATTGGGCAACA+AGG 0.548692 7.4:-22378758 MS.gene033758:CDS
AATGTGTTCACTGGAGGCCA+AGG 0.551160 7.4:-22379559 MS.gene033758:CDS
TGTGGACAATATTCCAATCA+GGG 0.551213 7.4:-22378856 MS.gene033758:CDS
TCAAAAGCACCATTCATAGC+AGG 0.555389 7.4:-22378711 MS.gene033758:CDS
CTTCAGCATGAACATAAAGA+TGG 0.562772 7.4:-22379870 MS.gene033758:CDS
GTGGATTAGAGAAAACTGAT+TGG 0.570924 7.4:-22378734 MS.gene033758:CDS
CGGGGTGGCGCTGAAAGCCG+CGG 0.571142 7.4:+22380188 None:intergenic
GATGATTGGGCAACAAGGGG+TGG 0.574149 7.4:-22378753 MS.gene033758:CDS
TACATCTACCGGCCTTTGCG+GGG 0.574701 7.4:+22380170 None:intergenic
GCATCAAGGTCACGAAACTC+AGG 0.577476 7.4:+22378603 None:intergenic
CTTCTTCTTGATAAGTACAC+TGG 0.587312 7.4:-22380072 MS.gene033758:intron
ATCTACCGGCCTTTGCGGGG+TGG 0.599027 7.4:+22380173 None:intergenic
TAGAACTGAGTATCTATGGT+AGG 0.601747 7.4:+22379492 None:intergenic
GCAAAGTACTGTGCAAAACA+AGG 0.603844 7.4:-22378645 MS.gene033758:CDS
TACATTCAGGAGGTATCTGA+GGG 0.605395 7.4:+22378505 None:intergenic
TCGAAACTATGTACCCACTT+GGG 0.605536 7.4:-22380139 MS.gene033758:CDS
GCAGTTATCTTCCCAAAATG+CGG 0.624739 7.4:-22379647 MS.gene033758:intron
CAAAACAAGGTAGAAAATGG+TGG 0.627129 7.4:-22378632 MS.gene033758:CDS
CAAACAAATGTGTTCACTGG+AGG 0.629079 7.4:-22379565 MS.gene033758:CDS
TTTGTTGTGTCTAACGTCCG+CGG 0.640803 7.4:-22380205 MS.gene033758:CDS
CAAGGTCACGAAACTCAGGT+TGG 0.649125 7.4:+22378607 None:intergenic
GCAGATGATTGGGCAACAAG+GGG 0.654510 7.4:-22378756 MS.gene033758:CDS
ACTGTTCCAGCTGAATCACC+AGG 0.657325 7.4:+22379845 None:intergenic
ACAGTACTTTGCATTAACTG+AGG 0.671350 7.4:+22378656 None:intergenic
CGGTCTCTTCTACATTCAGG+AGG 0.676223 7.4:+22378495 None:intergenic
ATGTGATCAAAAGCCCAAGT+GGG 0.680372 7.4:+22380126 None:intergenic
TCAATGGAAAAGGATAAAGT+GGG 0.694713 7.4:-22378577 MS.gene033758:CDS
GATGTGATCAAAAGCCCAAG+TGG 0.698619 7.4:+22380125 None:intergenic
AGGTTTCACAACCATCAATG+TGG 0.700769 7.4:+22378676 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAATATATGTCTTAT+TGG - chr7.4:22378722-22378741 MS.gene033758:CDS 10.0%
!!! TATTTATACAATTTTTGAAT+AGG - chr7.4:22378790-22378809 MS.gene033758:CDS 10.0%
!! ACATTTGTTTGTAAAATATA+AGG + chr7.4:22379148-22379167 None:intergenic 15.0%
!! ATCCCTAAATAAATAAATTT+CGG + chr7.4:22379537-22379556 None:intergenic 15.0%
!! GTTAATTAAGATTCTATATA+CGG + chr7.4:22379476-22379495 None:intergenic 15.0%
!! TTAATTAAGATTCTATATAC+GGG + chr7.4:22379475-22379494 None:intergenic 15.0%
!! TTCTACATAGAAATTAATTA+AGG + chr7.4:22378692-22378711 None:intergenic 15.0%
!! AAATTAATCATCTTTCATGA+AGG + chr7.4:22379679-22379698 None:intergenic 20.0%
!! AATTAATCATCTTTCATGAA+GGG + chr7.4:22379678-22379697 None:intergenic 20.0%
!! AGACCGAAATTTATTTATTT+AGG - chr7.4:22379531-22379550 MS.gene033758:CDS 20.0%
!! ATCAAAACGTCATATAAATT+AGG + chr7.4:22379756-22379775 None:intergenic 20.0%
!! ATCAGTACAGTAATTATAAA+TGG + chr7.4:22380186-22380205 None:intergenic 20.0%
!! GACCGAAATTTATTTATTTA+GGG - chr7.4:22379532-22379551 MS.gene033758:CDS 20.0%
!!! CTATCATAGTAATTTTTTAC+TGG - chr7.4:22379708-22379727 MS.gene033758:intron 20.0%
!!! GAAATAGATTTTGAGTTTTT+GGG - chr7.4:22379106-22379125 MS.gene033758:intron 20.0%
!!! TGAAATAGATTTTGAGTTTT+TGG - chr7.4:22379105-22379124 MS.gene033758:intron 20.0%
! AAACGTCATATAAATTAGGA+CGG + chr7.4:22379752-22379771 None:intergenic 25.0%
! AAATTCACATATGTATGTGT+AGG - chr7.4:22379838-22379857 MS.gene033758:CDS 25.0%
! CACATCAAATACTTTAATGA+AGG - chr7.4:22378614-22378633 MS.gene033758:CDS 25.0%
! TAGATAGAAAGATACTAACA+CGG - chr7.4:22379365-22379384 MS.gene033758:intron 25.0%
!! AATTGATCCAATTTTGTAGT+GGG - chr7.4:22378978-22378997 MS.gene033758:intron 25.0%
!! TACAATTTTTGAATAGGTAC+TGG - chr7.4:22378796-22378815 MS.gene033758:CDS 25.0%
!!! GTTTTGAAGTCTTTCTTAAT+AGG - chr7.4:22379406-22379425 MS.gene033758:intron 25.0%
AAACTGTTGTATACCTTCAT+TGG + chr7.4:22379939-22379958 None:intergenic 30.0%
AATTAACCCCACTACAAAAT+TGG + chr7.4:22378988-22379007 None:intergenic 30.0%
ACAAGTATGATCCTTTAGAT+TGG + chr7.4:22378825-22378844 None:intergenic 30.0%
CAAATCAGACAAAACTTATG+GGG + chr7.4:22379287-22379306 None:intergenic 30.0%
CCAAATCAGACAAAACTTAT+GGG + chr7.4:22379288-22379307 None:intergenic 30.0%
GAGAACAAAGAATATTTCTC+TGG - chr7.4:22379191-22379210 MS.gene033758:intron 30.0%
TACAAAAGTTTCCACATTGA+TGG - chr7.4:22380035-22380054 MS.gene033758:intron 30.0%
TCAATGGAAAAGGATAAAGT+GGG - chr7.4:22380145-22380164 MS.gene033758:CDS 30.0%
TCTAAAGGATCATACTTGTT+TGG - chr7.4:22378826-22378845 MS.gene033758:CDS 30.0%
TTACAAACAAATGTGTTCAC+TGG - chr7.4:22379154-22379173 MS.gene033758:intron 30.0%
TTCAAAAACAGCAAGAAGTT+AGG - chr7.4:22379891-22379910 MS.gene033758:CDS 30.0%
TTCTTTGTTCTCTATTACCT+TGG + chr7.4:22379183-22379202 None:intergenic 30.0%
TTTATGGAATGCAGATGATT+GGG - chr7.4:22379956-22379975 MS.gene033758:intron 30.0%
! ATTGATCCAATTTTGTAGTG+GGG - chr7.4:22378979-22378998 MS.gene033758:intron 30.0%
! GAATTGATCCAATTTTGTAG+TGG - chr7.4:22378977-22378996 MS.gene033758:intron 30.0%
! TGAAGGTATACAACAGTTTA+TGG - chr7.4:22379940-22379959 MS.gene033758:intron 30.0%
! TTTTGTGCATACATAGAAAG+CGG + chr7.4:22378905-22378924 None:intergenic 30.0%
!! CCATAAGTTTTGTCTGATTT+GGG - chr7.4:22379286-22379305 MS.gene033758:intron 30.0%
ACAGTACTTTGCATTAACTG+AGG + chr7.4:22380069-22380088 None:intergenic 35.0%
ACCATAGATACTCAGTTCTA+TGG - chr7.4:22379233-22379252 MS.gene033758:intron 35.0%
ATAGGATGACTAAAATCGCA+TGG - chr7.4:22379424-22379443 MS.gene033758:intron 35.0%
ATGAACATAAAGATGGTTCC+TGG - chr7.4:22378859-22378878 MS.gene033758:CDS 35.0%
ATGTATGTGTAGGTTCTTTG+TGG - chr7.4:22379848-22379867 MS.gene033758:CDS 35.0%
CAAAACAAGGTAGAAAATGG+TGG - chr7.4:22380090-22380109 MS.gene033758:CDS 35.0%
CCCAAATCAGACAAAACTTA+TGG + chr7.4:22379289-22379308 None:intergenic 35.0%
CTCAATGGAAAAGGATAAAG+TGG - chr7.4:22380144-22380163 MS.gene033758:CDS 35.0%
CTTCAGCATGAACATAAAGA+TGG - chr7.4:22378852-22378871 MS.gene033758:CDS 35.0%
CTTCTTCTTGATAAGTACAC+TGG - chr7.4:22378650-22378669 MS.gene033758:CDS 35.0%
GTGCAAAACAAGGTAGAAAA+TGG - chr7.4:22380087-22380106 MS.gene033758:CDS 35.0%
GTTTATGGAATGCAGATGAT+TGG - chr7.4:22379955-22379974 MS.gene033758:intron 35.0%
TAGAACTGAGTATCTATGGT+AGG + chr7.4:22379233-22379252 None:intergenic 35.0%
TCCATAGAACTGAGTATCTA+TGG + chr7.4:22379237-22379256 None:intergenic 35.0%
TCCATTTGATCAACCAATGA+AGG - chr7.4:22379923-22379942 MS.gene033758:intron 35.0%
TCTTTATGTTCATGCTGAAG+TGG + chr7.4:22378852-22378871 None:intergenic 35.0%
TGTGGACAATATTCCAATCA+GGG - chr7.4:22379866-22379885 MS.gene033758:CDS 35.0%
TTGTGGACAATATTCCAATC+AGG - chr7.4:22379865-22379884 MS.gene033758:CDS 35.0%
! ACCTTCATTGGTTGATCAAA+TGG + chr7.4:22379927-22379946 None:intergenic 35.0%
! CTTTTGTAACCTGCTATGAA+TGG + chr7.4:22380023-22380042 None:intergenic 35.0%
! TATCTATGGTAGGCTTTTGT+AGG + chr7.4:22379223-22379242 None:intergenic 35.0%
! TTCATCATGTTCCGCATTTT+GGG + chr7.4:22379089-22379108 None:intergenic 35.0%
! TTTCATCATGTTCCGCATTT+TGG + chr7.4:22379090-22379109 None:intergenic 35.0%
!! CCCATAAGTTTTGTCTGATT+TGG - chr7.4:22379285-22379304 MS.gene033758:intron 35.0%
!! GTGGATTAGAGAAAACTGAT+TGG - chr7.4:22379988-22380007 MS.gene033758:intron 35.0%
AGGTTTCACAACCATCAATG+TGG + chr7.4:22380049-22380068 None:intergenic 40.0%
ATGTGATCAAAAGCCCAAGT+GGG + chr7.4:22378599-22378618 None:intergenic 40.0%
CAAAACTCCAAGTCAAAGCA+TGG + chr7.4:22379392-22379411 None:intergenic 40.0%
CAAACAAATGTGTTCACTGG+AGG - chr7.4:22379157-22379176 MS.gene033758:intron 40.0%
GCAGTTATCTTCCCAAAATG+CGG - chr7.4:22379075-22379094 MS.gene033758:intron 40.0%
GGTACATAGTTTCGACCAAA+AGG + chr7.4:22378578-22378597 None:intergenic 40.0%
TCGAAACTATGTACCCACTT+GGG - chr7.4:22378583-22378602 MS.gene033758:CDS 40.0%
TTGATGCTGCTCAATGGAAA+AGG - chr7.4:22380135-22380154 MS.gene033758:CDS 40.0%
! GATAAGTTGAAACGTGAGTC+AGG - chr7.4:22379574-22379593 MS.gene033758:CDS 40.0%
! TTTTCCATTGAGCAGCATCA+AGG + chr7.4:22380136-22380155 None:intergenic 40.0%
!! GCAAAGTACTGTGCAAAACA+AGG - chr7.4:22380077-22380096 MS.gene033758:CDS 40.0%
!! TACATTCAGGAGGTATCTGA+GGG + chr7.4:22380220-22380239 None:intergenic 40.0%
!! TCAAAAGCACCATTCATAGC+AGG - chr7.4:22380011-22380030 MS.gene033758:intron 40.0%
!!! CTGTTTTTGAACACCCTGAT+TGG + chr7.4:22379882-22379901 None:intergenic 40.0%
!!! GAGTTTTTGGGTAACAGAAC+AGG - chr7.4:22379118-22379137 MS.gene033758:intron 40.0%
ATGCAGATGATTGGGCAACA+AGG - chr7.4:22379964-22379983 MS.gene033758:intron 45.0%
GATGTGATCAAAAGCCCAAG+TGG + chr7.4:22378600-22378619 None:intergenic 45.0%
GTCGAAACTATGTACCCACT+TGG - chr7.4:22378582-22378601 MS.gene033758:CDS 45.0%
TCACGGTCTCTTCTACATTC+AGG + chr7.4:22380233-22380252 None:intergenic 45.0%
TGCAGATGATTGGGCAACAA+GGG - chr7.4:22379965-22379984 MS.gene033758:intron 45.0%
! CGACCAAAAGGTACATCTAC+CGG + chr7.4:22378566-22378585 None:intergenic 45.0%
! TTTGTTGTGTCTAACGTCCG+CGG - chr7.4:22378517-22378536 MS.gene033758:CDS 45.0%
!! CTACATTCAGGAGGTATCTG+AGG + chr7.4:22380221-22380240 None:intergenic 45.0%
!! GGACGGAGAAAGTACTATGA+TGG + chr7.4:22379735-22379754 None:intergenic 45.0%
!! GGTACTGGCTTCCAATCTAA+AGG - chr7.4:22378811-22378830 MS.gene033758:CDS 45.0%
AATGTGTTCACTGGAGGCCA+AGG - chr7.4:22379163-22379182 MS.gene033758:intron 50.0%
ACACGGTCCATGCTTTGACT+TGG - chr7.4:22379382-22379401 MS.gene033758:intron 50.0%
ACTGTTCCAGCTGAATCACC+AGG + chr7.4:22378880-22378899 None:intergenic 50.0%
ATGGTTCCTGGTGATTCAGC+TGG - chr7.4:22378871-22378890 MS.gene033758:intron 50.0%
CAAGGTCACGAAACTCAGGT+TGG + chr7.4:22380118-22380137 None:intergenic 50.0%
CGGTCTCTTCTACATTCAGG+AGG + chr7.4:22380230-22380249 None:intergenic 50.0%
GCAGATGATTGGGCAACAAG+GGG - chr7.4:22379966-22379985 MS.gene033758:intron 50.0%
GCATCAAGGTCACGAAACTC+AGG + chr7.4:22380122-22380141 None:intergenic 50.0%
GGGATATCACGTGCACAACA+TGG - chr7.4:22379552-22379571 MS.gene033758:CDS 50.0%
GTGACCTTGATGCTGCTCAA+TGG - chr7.4:22380129-22380148 MS.gene033758:CDS 50.0%
! AGGCCGGTAGATGTACCTTT+TGG - chr7.4:22378560-22378579 MS.gene033758:CDS 50.0%
! TGGGGTTTTCGTCGTGGATT+TGG - chr7.4:22378486-22378505 MS.gene033758:CDS 50.0%
GATGATTGGGCAACAAGGGG+TGG - chr7.4:22379969-22379988 MS.gene033758:intron 55.0%
GGTACATCTACCGGCCTTTG+CGG + chr7.4:22378557-22378576 None:intergenic 55.0%
GTACATCTACCGGCCTTTGC+GGG + chr7.4:22378556-22378575 None:intergenic 55.0%
TACATCTACCGGCCTTTGCG+GGG + chr7.4:22378555-22378574 None:intergenic 55.0%
ATCTACCGGCCTTTGCGGGG+TGG + chr7.4:22378552-22378571 None:intergenic 65.0%
CTTTCAGCGCCACCCCGCAA+AGG - chr7.4:22378540-22378559 MS.gene033758:CDS 65.0%
CAGCGCCACCCCGCAAAGGC+CGG - chr7.4:22378544-22378563 MS.gene033758:CDS 75.0%
! CGGGGTGGCGCTGAAAGCCG+CGG + chr7.4:22378537-22378556 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 22378485 22380259 22378485 ID=MS.gene033758
chr7.4 mRNA 22378485 22380259 22378485 ID=MS.gene033758.t1;Parent=MS.gene033758
chr7.4 exon 22380073 22380259 22380073 ID=MS.gene033758.t1.exon1;Parent=MS.gene033758.t1
chr7.4 CDS 22380073 22380259 22380073 ID=cds.MS.gene033758.t1;Parent=MS.gene033758.t1
chr7.4 exon 22379832 22379932 22379832 ID=MS.gene033758.t1.exon2;Parent=MS.gene033758.t1
chr7.4 CDS 22379832 22379932 22379832 ID=cds.MS.gene033758.t1;Parent=MS.gene033758.t1
chr7.4 exon 22379472 22379665 22379472 ID=MS.gene033758.t1.exon3;Parent=MS.gene033758.t1
chr7.4 CDS 22379472 22379665 22379472 ID=cds.MS.gene033758.t1;Parent=MS.gene033758.t1
chr7.4 exon 22378485 22378884 22378485 ID=MS.gene033758.t1.exon4;Parent=MS.gene033758.t1
chr7.4 CDS 22378485 22378884 22378485 ID=cds.MS.gene033758.t1;Parent=MS.gene033758.t1
Gene Sequence

>MS.gene033758

ATGGGGTTTTCGTCGTGGATTTGGTCTCTAACTTTGTTGTGTCTAACGTCCGCGGCTTTCAGCGCCACCCCGCAAAGGCCGGTAGATGTACCTTTTGGTCGAAACTATGTACCCACTTGGGCTTTTGATCACATCAAATACTTTAATGAAGGTTCTGAGATTGAACTTCTTCTTGATAAGTACACTGGTGAGATACTACATGTTCCTTAATTAATTTCTATGTAGAACAAAGTTCAATTAATTAATATATGTCTTATTGGTTAATTTAATTGCAAACTTGATATTATTATTGTTTATGTTTAATTTATTTATACAATTTTTGAATAGGTACTGGCTTCCAATCTAAAGGATCATACTTGTTTGGCCACTTCAGCATGAACATAAAGATGGTTCCTGGTGATTCAGCTGGAACAGTCACCGCTTTCTATGTATGCACAAAAATTATTTTATGAAAAATATATTTTATTTAATTTAATTTTAAATTTTATGTTTGAATTGATCCAATTTTGTAGTGGGGTTAATTCTATAGTTTATGTGTTTGTAACTTGTATTGAATGTAACATTTGCTAATTATATATGAATTAAATGTTGCAGTTATCTTCCCAAAATGCGGAACATGATGAAATAGATTTTGAGTTTTTGGGTAACAGAACAGGACAACCTTATATTTTACAAACAAATGTGTTCACTGGAGGCCAAGGTAATAGAGAACAAAGAATATTTCTCTGGTTTGATCCTACAAAAGCCTACCATAGATACTCAGTTCTATGGAACACGTATCAAATTGTGTAAGTTACTACCCCATAAGTTTTGTCTGATTTGGGAATATATATTAAAATAAAGTAAAAATACAAGTTTTCATAAATGTTCTTTGTTGTTGTAGATAGAAAGATACTAACACGGTCCATGCTTTGACTTGGAGTTTTGAAGTCTTTCTTAATAGGATGACTAAAATCGCATGGCTATGTGAATATTGAATTATTGTGACCCGTATATAGAATCTTAATTAACTAACTAGTTGAGAGTATACTCAACACTTATATATAAGACCGAAATTTATTTATTTAGGGATATCACGTGCACAACATGGATAAGTTGAAACGTGAGTCAGGTTCAATTGTGTTGACTAAATCATGATAATTAAAAAAAATTGATACTAATATTAAATTTGTTAGTAATTTACATTGTTTCCCTTCATGAAAGATGATTAATTTATCTTCTTTCTATCATAGTAATTTTTTACTGGTCCATCATAGTACTTTCTCCGTCCTAATTTATATGACGTTTTGATCATTTCACACGTATTAAGAAACTTAATATATATGATAAGTAAACTAAATATATCAATAACAAAAATTCACATATGTATGTGTAGGTTCTTTGTGGACAATATTCCAATCAGGGTGTTCAAAAACAGCAAGAAGTTAGGAGTGAAATTTCCATTTGATCAACCAATGAAGGTATACAACAGTTTATGGAATGCAGATGATTGGGCAACAAGGGGTGGATTAGAGAAAACTGATTGGTCAAAAGCACCATTCATAGCAGGTTACAAAAGTTTCCACATTGATGGTTGTGAAACCTCAGTTAATGCAAAGTACTGTGCAAAACAAGGTAGAAAATGGTGGAACCAACCTGAGTTTCGTGACCTTGATGCTGCTCAATGGAAAAGGATAAAGTGGGTTCGTAAGAAATTCACCATTTATAATTACTGTACTGATAGAACACGTTTCCCTCAGATACCTCCTGAATGTAGAAGAGACCGTGACATTTAA
Protein sequence

>MS.gene033758.t1

MGFSSWIWSLTLLCLTSAAFSATPQRPVDVPFGRNYVPTWAFDHIKYFNEGSEIELLLDKYTGTGFQSKGSYLFGHFSMNIKMVPGDSAGTVTAFYLSSQNAEHDEIDFEFLGNRTGQPYILQTNVFTGGQGNREQRIFLWFDPTKAYHRYSVLWNTYQIVFFVDNIPIRVFKNSKKLGVKFPFDQPMKVYNSLWNADDWATRGGLEKTDWSKAPFIAGYKSFHIDGCETSVNAKYCAKQGRKWWNQPEFRDLDAAQWKRIKWVRKKFTIYNYCTDRTRFPQIPPECRRDRDI