AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31221

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31221.t1 MTR_2g095800 95.890 292 12 0 1 292 18 309 0.0 592
MS.gene31221.t1 MTR_4g057450 77.816 293 63 2 1 292 13 304 7.69e-172 478
MS.gene31221.t1 MTR_1g063020 67.474 289 92 2 4 292 8 294 4.26e-146 412
MS.gene31221.t1 MTR_7g102260 67.010 291 94 2 3 292 2 291 1.87e-145 410
MS.gene31221.t1 MTR_1g033420 41.137 299 155 7 1 292 7 291 2.62e-69 218
MS.gene31221.t1 MTR_8g064180 40.066 302 164 8 5 292 12 310 2.29e-64 204
MS.gene31221.t1 MTR_6g088320 39.179 268 143 7 36 292 25 283 3.36e-61 196
MS.gene31221.t1 MTR_4g126920 39.850 266 143 6 36 292 24 281 3.24e-59 191
MS.gene31221.t1 MTR_4g045853 36.422 313 153 8 1 292 1 288 6.94e-59 190
MS.gene31221.t1 MTR_5g029100 37.132 272 157 5 32 292 37 305 1.12e-58 191
MS.gene31221.t1 MTR_3g089540 41.418 268 132 8 36 292 29 282 1.49e-58 189
MS.gene31221.t1 MTR_5g091730 39.326 267 137 8 36 292 27 278 7.71e-58 187
MS.gene31221.t1 MTR_4g128410 37.500 296 159 8 5 292 3 280 8.45e-58 187
MS.gene31221.t1 MTR_8g090100 37.943 282 148 7 27 292 11 281 1.26e-57 187
MS.gene31221.t1 MTR_1g105495 36.486 296 170 7 8 292 6 294 9.20e-57 185
MS.gene31221.t1 MTR_7g084770 36.552 290 164 7 13 292 8 287 1.73e-56 184
MS.gene31221.t1 MTR_2g089140 36.364 275 153 5 32 292 20 286 3.67e-56 183
MS.gene31221.t1 MTR_7g093530 37.838 296 157 7 5 292 3 279 4.24e-56 182
MS.gene31221.t1 MTR_3g084630 38.971 272 146 7 29 292 18 277 1.30e-55 181
MS.gene31221.t1 MTR_3g084620 38.971 272 146 7 29 292 18 277 1.31e-55 181
MS.gene31221.t1 MTR_7g084750 36.364 297 168 9 5 292 3 287 2.04e-55 181
MS.gene31221.t1 MTR_5g029100 38.983 236 132 4 66 292 42 274 5.76e-55 181
MS.gene31221.t1 MTR_7g084760 37.687 268 147 7 35 292 30 287 8.07e-55 179
MS.gene31221.t1 MTR_8g013055 39.552 268 141 7 35 292 29 285 1.17e-54 179
MS.gene31221.t1 MTR_4g128570 39.924 263 132 8 36 292 30 272 1.37e-54 178
MS.gene31221.t1 MTR_8g081040 40.074 272 144 7 29 292 21 281 1.49e-54 179
MS.gene31221.t1 MTR_2g038860 38.519 270 145 6 36 292 25 286 7.20e-54 177
MS.gene31221.t1 MTR_2g038705 38.519 270 145 6 36 292 25 286 7.20e-54 177
MS.gene31221.t1 MTR_4g097640 35.452 299 157 11 7 292 12 287 8.22e-54 177
MS.gene31221.t1 MTR_4g097670 35.452 299 157 11 7 292 12 287 8.22e-54 177
MS.gene31221.t1 MTR_4g128580 38.662 269 145 6 36 292 25 285 1.75e-53 176
MS.gene31221.t1 MTR_8g102910 37.079 267 150 6 36 292 21 279 2.28e-53 176
MS.gene31221.t1 MTR_4g097700 37.313 268 146 9 36 292 68 324 5.02e-53 176
MS.gene31221.t1 MTR_6g033085 36.940 268 149 8 35 292 30 287 6.69e-53 174
MS.gene31221.t1 MTR_1g105900 35.766 274 158 8 29 292 26 291 6.01e-52 172
MS.gene31221.t1 MTR_6g033055 36.194 268 151 8 35 292 30 287 2.33e-51 171
MS.gene31221.t1 MTR_4g128400 37.891 256 143 6 45 292 36 283 3.29e-51 170
MS.gene31221.t1 MTR_7g056090 36.395 294 153 11 6 292 15 281 5.54e-50 167
MS.gene31221.t1 MTR_8g102220 37.500 272 138 9 29 292 19 266 4.66e-49 164
MS.gene31221.t1 MTR_2g089140 37.991 229 120 5 78 292 3 223 2.64e-48 160
MS.gene31221.t1 MTR_4g128590 36.531 271 133 9 36 292 30 275 1.46e-47 160
MS.gene31221.t1 MTR_2g049130 33.465 254 150 8 34 279 26 268 3.94e-44 152
MS.gene31221.t1 MTR_2g049030 33.333 270 153 12 32 292 25 276 3.76e-42 146
MS.gene31221.t1 MTR_8g032640 33.333 180 107 5 56 233 32 200 4.18e-18 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31221.t1 AT2G36870 66.327 294 96 2 1 292 5 297 7.38e-144 406
MS.gene31221.t1 AT3G44990 64.384 292 99 2 5 292 3 293 4.89e-138 392
MS.gene31221.t1 AT2G36870 66.092 174 57 1 1 172 5 178 3.81e-79 238
MS.gene31221.t1 AT1G14720 39.927 273 154 4 27 292 21 290 8.24e-69 217
MS.gene31221.t1 AT1G32170 40.074 272 151 4 30 292 25 293 2.30e-65 208
MS.gene31221.t1 AT2G01850 40.977 266 143 5 36 292 30 290 1.03e-64 206
MS.gene31221.t1 AT1G10550 35.971 278 161 3 29 292 35 309 1.71e-62 200
MS.gene31221.t1 AT4G18990 38.652 282 148 5 32 292 35 312 5.98e-62 200
MS.gene31221.t1 AT3G25050 37.736 265 150 4 35 292 33 289 2.30e-61 196
MS.gene31221.t1 AT3G23730 40.892 269 139 8 36 292 26 286 2.74e-60 194
MS.gene31221.t1 AT4G14130 41.791 268 135 8 36 292 27 284 7.69e-60 192
MS.gene31221.t1 AT1G11545 37.908 306 156 10 1 292 14 299 8.79e-60 193
MS.gene31221.t1 AT4G03210 35.644 303 157 7 4 292 6 284 1.26e-59 192
MS.gene31221.t1 AT5G57550 40.784 255 135 7 36 284 29 273 2.18e-59 191
MS.gene31221.t1 AT5G57560 39.259 270 145 8 34 292 20 281 7.75e-58 187
MS.gene31221.t1 AT4G25810 38.538 301 146 11 4 292 10 283 1.04e-57 187
MS.gene31221.t1 AT5G57530 40.809 272 139 9 32 292 22 282 8.53e-57 184
MS.gene31221.t1 AT4G13090 39.925 268 144 6 34 292 29 288 1.17e-56 184
MS.gene31221.t1 AT4G37800 36.149 296 164 7 6 292 10 289 4.78e-55 180
MS.gene31221.t1 AT4G13080 38.577 267 147 8 33 292 35 291 8.93e-55 179
MS.gene31221.t1 AT4G30270 37.984 258 135 7 32 284 19 256 1.67e-54 178
MS.gene31221.t1 AT5G57540 41.045 268 136 9 36 292 25 281 1.75e-54 178
MS.gene31221.t1 AT5G13870 35.811 296 166 9 6 292 7 287 1.77e-54 179
MS.gene31221.t1 AT5G13870 35.811 296 166 9 6 292 7 287 1.77e-54 179
MS.gene31221.t1 AT5G13870 35.811 296 166 9 6 292 7 287 1.77e-54 179
MS.gene31221.t1 AT5G65730 38.258 264 149 6 36 292 34 290 2.46e-54 178
MS.gene31221.t1 AT5G48070 38.603 272 146 10 30 292 22 281 6.77e-54 177
MS.gene31221.t1 AT2G06850 36.861 274 155 7 28 292 26 290 3.62e-53 176
MS.gene31221.t1 AT4G28850 35.075 268 153 8 37 292 29 287 2.53e-52 173
MS.gene31221.t1 AT1G65310 40.000 265 140 8 36 292 28 281 2.59e-52 173
MS.gene31221.t1 AT1G65310 40.000 265 140 8 36 292 50 303 4.87e-52 173
MS.gene31221.t1 AT4G03210 38.333 240 120 6 67 292 31 256 9.00e-52 171
MS.gene31221.t1 AT2G18800 36.462 277 147 9 37 292 28 296 9.42e-52 172
MS.gene31221.t1 AT4G25820 39.852 271 141 9 33 292 26 285 2.08e-51 171
MS.gene31221.t1 AT4G30290 40.693 231 123 6 66 292 56 276 2.79e-51 170
MS.gene31221.t1 AT2G14620 34.228 298 167 9 5 292 16 294 2.18e-50 168
MS.gene31221.t1 AT4G30280 39.216 255 139 5 36 284 28 272 4.29e-50 167
MS.gene31221.t1 AT2G06850 38.723 235 128 6 65 292 5 230 8.24e-50 165
MS.gene31221.t1 AT4G18990 39.631 217 108 4 95 292 26 238 1.92e-48 163
MS.gene31221.t1 AT2G14620 36.596 235 133 7 66 292 18 244 1.60e-45 154
MS.gene31221.t1 AT2G14620 37.220 223 124 7 78 292 19 233 3.64e-43 148
MS.gene31221.t1 AT3G48580 33.206 262 151 8 35 292 32 273 2.22e-40 142
MS.gene31221.t1 AT3G48580 34.199 231 129 7 65 292 4 214 3.06e-37 132
MS.gene31221.t1 AT3G48580 29.389 262 141 9 35 292 32 253 7.50e-25 100

Find 73 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTACAAATCTGGATATTT+TGG 0.149428 2.4:-7015961 MS.gene31221:CDS
CATAGACCAAGTAATTATTT+TGG 0.207651 2.4:+7014516 None:intergenic
TTCCTAGGATACCTCCTTAT+TGG 0.217615 2.4:+7014788 None:intergenic
ATCAAGAATTTAGAAATCTA+TGG 0.228439 2.4:-7016484 MS.gene31221:CDS
GAGAGATGAGATTTCATCTT+TGG 0.246832 2.4:-7015664 MS.gene31221:CDS
TTCACAACTATGCCATTCTA+TGG 0.276482 2.4:-7015622 MS.gene31221:CDS
CGAACTAAACCTTAGCATTC+TGG 0.321947 2.4:+7014455 None:intergenic
AGTGGAGATGGGAAGATCAT+AGG 0.350619 2.4:-7015689 MS.gene31221:CDS
TATACTTACCAACCCTTCAT+TGG 0.351915 2.4:-7014668 MS.gene31221:CDS
GTAAGTGCATGGTCTCTCTT+AGG 0.376321 2.4:+7014479 None:intergenic
GCAATACATATGGCTTATCT+TGG 0.380400 2.4:+7015735 None:intergenic
TCATATCACTCTTACAAATC+TGG 0.381593 2.4:-7015970 MS.gene31221:CDS
ATGATTTGTATTAGTAATGC+TGG 0.382697 2.4:-7016563 MS.gene31221:CDS
TAGTGGATGATGTTCCAATA+AGG 0.386035 2.4:-7014802 MS.gene31221:CDS
GCTCAGCTTGCCGCCATAGC+CGG 0.389428 2.4:+7014568 None:intergenic
CAGACAAATGTGTACATTAG+AGG 0.390267 2.4:-7015713 MS.gene31221:CDS
ACATTTGTCTGCAATACATA+TGG 0.397108 2.4:+7015725 None:intergenic
CTCAGCTTGCCGCCATAGCC+GGG 0.408183 2.4:+7014569 None:intergenic
ACCATGAATCATTATCAATC+TGG 0.433994 2.4:-7016439 MS.gene31221:CDS
GCCAGATTGATAATGATTCA+TGG 0.436574 2.4:+7016438 None:intergenic
ATACTTACCAACCCTTCATT+GGG 0.448591 2.4:-7014667 MS.gene31221:CDS
CTCACTAGGTTTCCATAGAA+TGG 0.448681 2.4:+7015610 None:intergenic
TGATTTGTATTAGTAATGCT+GGG 0.449518 2.4:-7016562 MS.gene31221:CDS
TCTGTCTCACCTCCCGGCTA+TGG 0.450760 2.4:-7014581 MS.gene31221:CDS
TCAAGACCAATGTATGTGTA+TGG 0.458346 2.4:-7014746 MS.gene31221:CDS
ACATTAGAGGAAGTGGAGAT+GGG 0.459120 2.4:-7015700 MS.gene31221:CDS
AGCAAAATGTTGCAATGCAA+TGG 0.460888 2.4:-7014541 MS.gene31221:CDS
TACACATACATTGGTCTTGA+AGG 0.461246 2.4:+7014749 None:intergenic
TCTGCATGGGCTACAGAGAA+TGG 0.467528 2.4:-7014707 MS.gene31221:CDS
TCAAGAATTTAGAAATCTAT+GGG 0.469409 2.4:-7016483 MS.gene31221:CDS
TACATTAGAGGAAGTGGAGA+TGG 0.475103 2.4:-7015701 MS.gene31221:CDS
AATGTTGCATCACTCTTCCT+AGG 0.480500 2.4:+7014773 None:intergenic
AATGTGTACATTAGAGGAAG+TGG 0.491430 2.4:-7015707 MS.gene31221:CDS
AGGCGGACGGCATGAGGAGG+TGG 0.495521 2.4:+7014604 None:intergenic
GAAATAGACATTGAGTTCCT+TGG 0.499774 2.4:-7015764 MS.gene31221:CDS
ATCTGGCTTGACTCTACCTC+AGG 0.501094 2.4:-7016422 MS.gene31221:intron
ACTCAATGTCTATTTCATCA+TGG 0.503835 2.4:+7015771 None:intergenic
GCAAAATGTTGCAATGCAAT+GGG 0.506732 2.4:-7014540 MS.gene31221:CDS
CAATACATATGGCTTATCTT+GGG 0.512542 2.4:+7015736 None:intergenic
TTCCAATAAGGAGGTATCCT+AGG 0.523994 2.4:-7014790 MS.gene31221:CDS
CCGCCTTCTGTCTCACCTCC+CGG 0.526126 2.4:-7014587 MS.gene31221:CDS
TGTATCTCCCAATGAAGGGT+TGG 0.527457 2.4:+7014660 None:intergenic
CAATCTGGGATGCATCTGCA+TGG 0.536057 2.4:-7014721 MS.gene31221:CDS
ATGGGTCCAAAATAATTACT+TGG 0.539014 2.4:-7014522 MS.gene31221:CDS
TGTATGTGTATGGATCAATC+TGG 0.542899 2.4:-7014736 MS.gene31221:CDS
CTTCAACCAGGTTATACTGC+AGG 0.542998 2.4:-7015925 MS.gene31221:CDS
GAGAAGAAAAGAGAGAAGAG+AGG 0.561549 2.4:+7016590 None:intergenic
ACACATACATTGGTCTTGAA+GGG 0.561915 2.4:+7014750 None:intergenic
AAATATTACATGATCTCACT+AGG 0.565995 2.4:+7015596 None:intergenic
GCACTTACTCCAGAATGCTA+AGG 0.580492 2.4:-7014464 None:intergenic
GTCTCACCTCCCGGCTATGG+CGG 0.581515 2.4:-7014578 MS.gene31221:CDS
GACAGAAGGCGGACGGCATG+AGG 0.589748 2.4:+7014598 None:intergenic
CTAGGATAGTACCCTGGTGA+AGG 0.596166 2.4:+7016533 None:intergenic
AGAAGGCGGACGGCATGAGG+AGG 0.599752 2.4:+7014601 None:intergenic
ATTGATCCATACACATACAT+TGG 0.600084 2.4:+7014740 None:intergenic
TTGACTCCTGCAGTATAACC+TGG 0.602424 2.4:+7015919 None:intergenic
TAGTTGTGAAAATCTTGTGT+TGG 0.603177 2.4:+7015635 None:intergenic
TAGCCGGGAGGTGAGACAGA+AGG 0.606757 2.4:+7014584 None:intergenic
AATCTGGGATGCATCTGCAT+GGG 0.616394 2.4:-7014720 MS.gene31221:CDS
GCATGAGGAGGTGGAATCAG+TGG 0.618556 2.4:+7014613 None:intergenic
AGCATTCTGGAGTAAGTGCA+TGG 0.624643 2.4:+7014468 None:intergenic
AGCTTGCCGCCATAGCCGGG+AGG 0.627847 2.4:+7014572 None:intergenic
GATTTGTATTAGTAATGCTG+GGG 0.636926 2.4:-7016561 MS.gene31221:CDS
GTATGTGTATGGATCAATCT+GGG 0.642889 2.4:-7014735 MS.gene31221:CDS
TGGATGATGTTCCAATAAGG+AGG 0.647078 2.4:-7014799 MS.gene31221:CDS
GAGGTGAGACAGAAGGCGGA+CGG 0.648616 2.4:+7014591 None:intergenic
ACTGCGGTTAAGCTTCAACC+AGG 0.650106 2.4:-7015937 MS.gene31221:CDS
GCTTATCTTGGGTAGTACCA+AGG 0.651953 2.4:+7015747 None:intergenic
CCGGGAGGTGAGACAGAAGG+CGG 0.677362 2.4:+7014587 None:intergenic
TTAGAACTAGGATAGTACCC+TGG 0.696164 2.4:+7016527 None:intergenic
CAAGAATTTAGAAATCTATG+GGG 0.710687 2.4:-7016482 MS.gene31221:CDS
TCAAACATCGAAGATCACAA+AGG 0.711833 2.4:-7015797 MS.gene31221:CDS
ATTTGTATTAGTAATGCTGG+GGG 0.723446 2.4:-7016560 MS.gene31221:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACATTAATTCAAATTATA+AGG + chr2.4:7015192-7015211 None:intergenic 10.0%
!! ATGAGTATATATATTATAAA+AGG + chr2.4:7016144-7016163 None:intergenic 10.0%
!! TGAGTATATATATTATAAAA+GGG + chr2.4:7016143-7016162 None:intergenic 10.0%
!!! AATCTATTATTATTTTAGTA+TGG - chr2.4:7015019-7015038 MS.gene31221:intron 10.0%
!!! ATGTTTTAAAATAGTAAAAT+TGG + chr2.4:7015721-7015740 None:intergenic 10.0%
!!! ATTTTTAATAAAAACTGTTT+TGG + chr2.4:7015632-7015651 None:intergenic 10.0%
!!! TGTTGATATTAATTTAATTT+GGG - chr2.4:7015231-7015250 MS.gene31221:intron 10.0%
!! AATAAATAAAGAATCACATT+TGG + chr2.4:7014859-7014878 None:intergenic 15.0%
!! TTTCTCAAATTATATTAGAT+AGG + chr2.4:7014954-7014973 None:intergenic 15.0%
!!! ATAAAAATGATCTTTTTGAT+AGG + chr2.4:7016102-7016121 None:intergenic 15.0%
!!! GTGTTGATATTAATTTAATT+TGG - chr2.4:7015230-7015249 MS.gene31221:intron 15.0%
!!! TTATATATACAGATTTTTAG+TGG - chr2.4:7016243-7016262 MS.gene31221:intron 15.0%
!!! TTGATCTTTTTATTTCTATT+CGG + chr2.4:7015565-7015584 None:intergenic 15.0%
!!! TTTAGAATATATTATTTCTG+AGG + chr2.4:7015890-7015909 None:intergenic 15.0%
!! ATCAAGAATTTAGAAATCTA+TGG - chr2.4:7014578-7014597 MS.gene31221:CDS 20.0%
!! TCAAGAATTTAGAAATCTAT+GGG - chr2.4:7014579-7014598 MS.gene31221:CDS 20.0%
!! TCAATACAAATTTATATGTG+AGG - chr2.4:7015162-7015181 MS.gene31221:intron 20.0%
!! TCAATATTCTATGTTTAGTA+AGG - chr2.4:7015937-7015956 MS.gene31221:CDS 20.0%
!!! GTTAAATATTTTATTCCAAG+AGG - chr2.4:7016013-7016032 MS.gene31221:intron 20.0%
! AAATATTACATGATCTCACT+AGG + chr2.4:7015469-7015488 None:intergenic 25.0%
! ATCTAAACTAAAATCTCAAG+CGG - chr2.4:7014890-7014909 MS.gene31221:intron 25.0%
! ATTTCTCTATACCTATTAGT+AGG + chr2.4:7016062-7016081 None:intergenic 25.0%
! CAAGAATTTAGAAATCTATG+GGG - chr2.4:7014580-7014599 MS.gene31221:CDS 25.0%
! TACAAAAACTTCAAACTACA+AGG - chr2.4:7016421-7016440 MS.gene31221:intron 25.0%
! TTATGTATGACACTTCATTT+AGG + chr2.4:7014705-7014724 None:intergenic 25.0%
! TTTCTCTATACCTATTAGTA+GGG + chr2.4:7016061-7016080 None:intergenic 25.0%
!! ATGATTTGTATTAGTAATGC+TGG - chr2.4:7014499-7014518 MS.gene31221:CDS 25.0%
!! CATAGACCAAGTAATTATTT+TGG + chr2.4:7016549-7016568 None:intergenic 25.0%
!! TAGTTCAATTTGTTCAACTA+CGG - chr2.4:7015534-7015553 MS.gene31221:intron 25.0%
!! TCTTACAAATCTGGATATTT+TGG - chr2.4:7015101-7015120 MS.gene31221:intron 25.0%
!! TGATTTGTATTAGTAATGCT+GGG - chr2.4:7014500-7014519 MS.gene31221:CDS 25.0%
!! TTACTAATTTTCAGTTCGAT+TGG - chr2.4:7015805-7015824 MS.gene31221:intron 25.0%
!!! ATAGTGCTTATTTTAGAACT+AGG + chr2.4:7014550-7014569 None:intergenic 25.0%
!!! TATATTATTTCTGAGGATCT+TGG + chr2.4:7015883-7015902 None:intergenic 25.0%
!!! TTTTATTTAAAAGCCACATG+TGG - chr2.4:7016116-7016135 MS.gene31221:intron 25.0%
ACATTTGTCTGCAATACATA+TGG + chr2.4:7015340-7015359 None:intergenic 30.0%
ACCATGAATCATTATCAATC+TGG - chr2.4:7014623-7014642 MS.gene31221:CDS 30.0%
ACTCAATGTCTATTTCATCA+TGG + chr2.4:7015294-7015313 None:intergenic 30.0%
ATGGGTCCAAAATAATTACT+TGG - chr2.4:7016540-7016559 MS.gene31221:CDS 30.0%
ATTGATCCATACACATACAT+TGG + chr2.4:7016325-7016344 None:intergenic 30.0%
ATTTGTATTAGTAATGCTGG+GGG - chr2.4:7014502-7014521 MS.gene31221:CDS 30.0%
CAAATTGAACTATGATCTGT+TGG + chr2.4:7015526-7015545 None:intergenic 30.0%
CAATACATATGGCTTATCTT+GGG + chr2.4:7015329-7015348 None:intergenic 30.0%
CTAATAGGTATAGAGAAATG+AGG - chr2.4:7016063-7016082 MS.gene31221:intron 30.0%
GATTTGTATTAGTAATGCTG+GGG - chr2.4:7014501-7014520 MS.gene31221:CDS 30.0%
GCAAAAGTAAACAAAGAACT+AGG - chr2.4:7015763-7015782 MS.gene31221:CDS 30.0%
TAGTTGTGAAAATCTTGTGT+TGG + chr2.4:7015430-7015449 None:intergenic 30.0%
TCATATCACTCTTACAAATC+TGG - chr2.4:7015092-7015111 MS.gene31221:intron 30.0%
! ATTTTCCATACCCTACTAAT+AGG - chr2.4:7016048-7016067 MS.gene31221:intron 30.0%
! CATTTGATTTCATATACCTG+AGG + chr2.4:7014659-7014678 None:intergenic 30.0%
! GTGATTGATTGATAGCTATA+TGG + chr2.4:7015987-7016006 None:intergenic 30.0%
! TTTTTGTATCTCCCAATGAA+GGG + chr2.4:7016409-7016428 None:intergenic 30.0%
AAAACTAGACTGAGTCTGAA+AGG + chr2.4:7015845-7015864 None:intergenic 35.0%
AAGCATTTCTTTGCAACACA+GGG - chr2.4:7015057-7015076 MS.gene31221:intron 35.0%
AATGTGTACATTAGAGGAAG+TGG - chr2.4:7015355-7015374 MS.gene31221:intron 35.0%
AATTAAACACTCTTGCCTCT+TGG + chr2.4:7016031-7016050 None:intergenic 35.0%
ACACTTCATTTAGGTGAACT+TGG + chr2.4:7014696-7014715 None:intergenic 35.0%
AGCAAAATGTTGCAATGCAA+TGG - chr2.4:7016521-7016540 MS.gene31221:CDS 35.0%
ATACTTACCAACCCTTCATT+GGG - chr2.4:7016395-7016414 MS.gene31221:intron 35.0%
ATTATAAAAGGGACCACATG+TGG + chr2.4:7016132-7016151 None:intergenic 35.0%
CACTTCATTTAGGTGAACTT+GGG + chr2.4:7014695-7014714 None:intergenic 35.0%
CAGACAAATGTGTACATTAG+AGG - chr2.4:7015349-7015368 MS.gene31221:intron 35.0%
CTATACCTATTAGTAGGGTA+TGG + chr2.4:7016056-7016075 None:intergenic 35.0%
GAAATAGACATTGAGTTCCT+TGG - chr2.4:7015298-7015317 MS.gene31221:intron 35.0%
GAGAGATGAGATTTCATCTT+TGG - chr2.4:7015398-7015417 MS.gene31221:intron 35.0%
GCAAAATGTTGCAATGCAAT+GGG - chr2.4:7016522-7016541 MS.gene31221:CDS 35.0%
GCAATACATATGGCTTATCT+TGG + chr2.4:7015330-7015349 None:intergenic 35.0%
GCTATCAATCAATCACTGTT+TGG - chr2.4:7015991-7016010 MS.gene31221:intron 35.0%
GTATGTGTATGGATCAATCT+GGG - chr2.4:7016327-7016346 MS.gene31221:intron 35.0%
TAAGCATTTCTTTGCAACAC+AGG - chr2.4:7015056-7015075 MS.gene31221:intron 35.0%
TATACTTACCAACCCTTCAT+TGG - chr2.4:7016394-7016413 MS.gene31221:intron 35.0%
TCAAACATCGAAGATCACAA+AGG - chr2.4:7015265-7015284 MS.gene31221:intron 35.0%
TCAAGACCAATGTATGTGTA+TGG - chr2.4:7016316-7016335 MS.gene31221:intron 35.0%
TGTATGTGTATGGATCAATC+TGG - chr2.4:7016326-7016345 MS.gene31221:intron 35.0%
TTCACAACTATGCCATTCTA+TGG - chr2.4:7015440-7015459 MS.gene31221:intron 35.0%
! GTTTTTGTATCTCCCAATGA+AGG + chr2.4:7016410-7016429 None:intergenic 35.0%
! TAGTGGATGATGTTCCAATA+AGG - chr2.4:7016260-7016279 MS.gene31221:intron 35.0%
!! ACACATACATTGGTCTTGAA+GGG + chr2.4:7016315-7016334 None:intergenic 35.0%
!! GCCAGATTGATAATGATTCA+TGG + chr2.4:7014627-7014646 None:intergenic 35.0%
!! TACACATACATTGGTCTTGA+AGG + chr2.4:7016316-7016335 None:intergenic 35.0%
!!! ATCTGGATATTTTGGTACTG+CGG - chr2.4:7015109-7015128 MS.gene31221:intron 35.0%
AATGTTGCATCACTCTTCCT+AGG + chr2.4:7016292-7016311 None:intergenic 40.0%
ACAAACCAGTTAAACCAACC+TGG - chr2.4:7015652-7015671 MS.gene31221:CDS 40.0%
ACATTAGAGGAAGTGGAGAT+GGG - chr2.4:7015362-7015381 MS.gene31221:intron 40.0%
CATCAGGCTACATTAGAACT+AGG - chr2.4:7015688-7015707 MS.gene31221:CDS 40.0%
CTCACTAGGTTTCCATAGAA+TGG + chr2.4:7015455-7015474 None:intergenic 40.0%
GAGAAGAAAAGAGAGAAGAG+AGG + chr2.4:7014475-7014494 None:intergenic 40.0%
TAAACCAACCTGGTTGAGAA+TGG - chr2.4:7015662-7015681 MS.gene31221:CDS 40.0%
TACATTAGAGGAAGTGGAGA+TGG - chr2.4:7015361-7015380 MS.gene31221:intron 40.0%
TAGCGTGCTGAATTGCTTTA+TGG - chr2.4:7016219-7016238 MS.gene31221:intron 40.0%
TGGATGATGTTCCAATAAGG+AGG - chr2.4:7016263-7016282 MS.gene31221:intron 40.0%
TTAGAACTAGGATAGTACCC+TGG + chr2.4:7014538-7014557 None:intergenic 40.0%
TTCCTAGGATACCTCCTTAT+TGG + chr2.4:7016277-7016296 None:intergenic 40.0%
! GCATTGCAACATTTTGCTGA+CGG + chr2.4:7016519-7016538 None:intergenic 40.0%
!! CTAGTTCTAATGTAGCCTGA+TGG + chr2.4:7015690-7015709 None:intergenic 40.0%
!! TTCCAATAAGGAGGTATCCT+AGG - chr2.4:7016272-7016291 MS.gene31221:intron 40.0%
AGTGGAGATGGGAAGATCAT+AGG - chr2.4:7015373-7015392 MS.gene31221:intron 45.0%
CTGAGTCTGAAAGGATCGAT+CGG + chr2.4:7015836-7015855 None:intergenic 45.0%
CTTCAACCAGGTTATACTGC+AGG - chr2.4:7015137-7015156 MS.gene31221:intron 45.0%
GCTTATCTTGGGTAGTACCA+AGG + chr2.4:7015318-7015337 None:intergenic 45.0%
GTAAGTGCATGGTCTCTCTT+AGG + chr2.4:7016586-7016605 None:intergenic 45.0%
TGATGGAACCATTCTCAACC+AGG + chr2.4:7015673-7015692 None:intergenic 45.0%
TGGTTGAGAATGGTTCCATC+AGG - chr2.4:7015672-7015691 MS.gene31221:CDS 45.0%
TGTATCTCCCAATGAAGGGT+TGG + chr2.4:7016405-7016424 None:intergenic 45.0%
TTGACTCCTGCAGTATAACC+TGG + chr2.4:7015146-7015165 None:intergenic 45.0%
TTTCTTTGCAACACAGGGAG+TGG - chr2.4:7015062-7015081 MS.gene31221:intron 45.0%
! AATCTGGGATGCATCTGCAT+GGG - chr2.4:7016342-7016361 MS.gene31221:intron 45.0%
!! AGCATTCTGGAGTAAGTGCA+TGG + chr2.4:7016597-7016616 None:intergenic 45.0%
!! CTCAACCAGGTTGGTTTAAC+TGG + chr2.4:7015660-7015679 None:intergenic 45.0%
ACTGCGGTTAAGCTTCAACC+AGG - chr2.4:7015125-7015144 MS.gene31221:intron 50.0%
CTAGGATAGTACCCTGGTGA+AGG + chr2.4:7014532-7014551 None:intergenic 50.0%
GGAACCATTCTCAACCAGGT+TGG + chr2.4:7015669-7015688 None:intergenic 50.0%
! ATCTGGCTTGACTCTACCTC+AGG - chr2.4:7014640-7014659 MS.gene31221:CDS 50.0%
! CAATCTGGGATGCATCTGCA+TGG - chr2.4:7016341-7016360 MS.gene31221:intron 50.0%
! TCTGCATGGGCTACAGAGAA+TGG - chr2.4:7016355-7016374 MS.gene31221:intron 50.0%
GCATGAGGAGGTGGAATCAG+TGG + chr2.4:7016452-7016471 None:intergenic 55.0%
GAGGTGAGACAGAAGGCGGA+CGG + chr2.4:7016474-7016493 None:intergenic 60.0%
TAGCCGGGAGGTGAGACAGA+AGG + chr2.4:7016481-7016500 None:intergenic 60.0%
TCTGTCTCACCTCCCGGCTA+TGG - chr2.4:7016481-7016500 MS.gene31221:CDS 60.0%
AGAAGGCGGACGGCATGAGG+AGG + chr2.4:7016464-7016483 None:intergenic 65.0%
CCGCCTTCTGTCTCACCTCC+CGG - chr2.4:7016475-7016494 MS.gene31221:CDS 65.0%
CCGGGAGGTGAGACAGAAGG+CGG + chr2.4:7016478-7016497 None:intergenic 65.0%
CTCAGCTTGCCGCCATAGCC+GGG + chr2.4:7016496-7016515 None:intergenic 65.0%
GACAGAAGGCGGACGGCATG+AGG + chr2.4:7016467-7016486 None:intergenic 65.0%
GCTCAGCTTGCCGCCATAGC+CGG + chr2.4:7016497-7016516 None:intergenic 65.0%
GTCTCACCTCCCGGCTATGG+CGG - chr2.4:7016484-7016503 MS.gene31221:CDS 65.0%
! CTGGGGGTGCACCTTCACCA+GGG - chr2.4:7014518-7014537 MS.gene31221:CDS 65.0%
AGCTTGCCGCCATAGCCGGG+AGG + chr2.4:7016493-7016512 None:intergenic 70.0%
AGGCGGACGGCATGAGGAGG+TGG + chr2.4:7016461-7016480 None:intergenic 70.0%
! GCTGGGGGTGCACCTTCACC+AGG - chr2.4:7014517-7014536 MS.gene31221:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 7014466 7016618 7014466 ID=MS.gene31221
chr2.4 mRNA 7014466 7016618 7014466 ID=MS.gene31221.t1;Parent=MS.gene31221
chr2.4 exon 7016423 7016618 7016423 ID=MS.gene31221.t1.exon1;Parent=MS.gene31221.t1
chr2.4 CDS 7016423 7016618 7016423 ID=cds.MS.gene31221.t1;Parent=MS.gene31221.t1
chr2.4 exon 7015906 7016006 7015906 ID=MS.gene31221.t1.exon2;Parent=MS.gene31221.t1
chr2.4 CDS 7015906 7016006 7015906 ID=cds.MS.gene31221.t1;Parent=MS.gene31221.t1
chr2.4 exon 7015605 7015822 7015605 ID=MS.gene31221.t1.exon3;Parent=MS.gene31221.t1
chr2.4 CDS 7015605 7015822 7015605 ID=cds.MS.gene31221.t1;Parent=MS.gene31221.t1
chr2.4 exon 7014466 7014829 7014466 ID=MS.gene31221.t1.exon4;Parent=MS.gene31221.t1
chr2.4 CDS 7014466 7014829 7014466 ID=cds.MS.gene31221.t1;Parent=MS.gene31221.t1
Gene Sequence

>MS.gene31221

ATGGCTCCTCTCTTCTCTCTTTTCTTCTCATTCATGATTTGTATTAGTAATGCTGGGGGTGCACCTTCACCAGGGTACTATCCTAGTTCTAAAATAAGCACTATTAGCTTTGATCAAGAATTTAGAAATCTATGGGGAGCTCAGCATCAGAAACTAGACCATGAATCATTATCAATCTGGCTTGACTCTACCTCAGGTATATGAAATCAAATGTCTCAAATCATCTCCCAAGTTCACCTAAATGAAGTGTCATACATAATCATTAACTCGAAAAATAACTCTTGAAATTTCAAGCATTTCATAATTTGTTGCATGTGTACATAAATTTTGTTACAATTTTAATTTATTTCATCTTTGTACTATATAATACTCTTCTCAATTTTAGATGAGCCAAATGTGATTCTTTATTTATTTATTATTTGTAATCTAAACTAAAATCTCAAGCGGTTAAAACTCTTGATGAAGTACTTTGTCTTACACGTGTTCCTATCTAATATAATTTGAGAAATTAGTCTCTGCGACTACGTACATAGTACATAAAATATAATGCAAAAATCTATTATTATTTTAGTATGGATTCTTATTACTTCTAAGCATTTCTTTGCAACACAGGGAGTGGATTCAAGTCATATCACTCTTACAAATCTGGATATTTTGGTACTGCGGTTAAGCTTCAACCAGGTTATACTGCAGGAGTCAATACAAATTTATATGTGAGGATTACCTTATAATTTGAATTAATGTTTAAAATTGACTGTATATTTGTGTTGATATTAATTTAATTTGGGAATTGTAGCTTTCAAACATCGAAGATCACAAAGGAAACCATGATGAAATAGACATTGAGTTCCTTGGTACTACCCAAGATAAGCCATATGTATTGCAGACAAATGTGTACATTAGAGGAAGTGGAGATGGGAAGATCATAGGAAGAGAGATGAGATTTCATCTTTGGTTCGATCCAACACAAGATTTTCACAACTATGCCATTCTATGGAAACCTAGTGAGATCATGTAATATTTTCATACACTATTCTACAATGTTTTAAAATCTAGACCAACAGATCATAGTTCAATTTGTTCAACTACGGTTAAACCGAATAGAAATAAAAAGATCAAAAGTTTTTTTTTTTTGACAAAAGATCAAAAGTATTTAAAAAGTACCAAAACAGTTTTTATTAAAAATACAAACCAGTTAAACCAACCTGGTTGAGAATGGTTCCATCAGGCTACATTAGAACTAGGATTAAATCCAATTTTACTATTTTAAAACATATTTGAGTTTTTATATTCTAAAGCAAAAGTAAACAAAGAACTAGGATTAATTCGATCAATGAAGTTACTAATTTTCAGTTCGATTGGTTGAACCGATCGATCCTTTCAGACTCAGTCTAGTTTTTAAAACATTGTTATACCAAGATCCTCAGAAATAATATATTCTAAATATAAATATGACTTTATTTTGTTTGTGTCAATATTCTATGTTTAGTAAGGATCAACAAGTCAACAATCATTTAGCCATATAGCTATCAATCAATCACTGTTTGGTTAAATATTTTATTCCAAGAGGCAAGAGTGTTTAATTTTCCATACCCTACTAATAGGTATAGAGAAATGAGGTCTAGAAAATATTCCTATCAAAAAGATCATTTTTATTTAAAAGCCACATGTGGTCCCTTTTATAATATATATACTCATTAACATTATAATGTCATGTTTATTAAAAAACAAAGCATATAGCTATTTTTTTTGTTAGCGTGCTGAATTGCTTTATGGTTTATATATACAGATTTTTAGTGGATGATGTTCCAATAAGGAGGTATCCTAGGAAGAGTGATGCAACATTCCCTTCAAGACCAATGTATGTGTATGGATCAATCTGGGATGCATCTGCATGGGCTACAGAGAATGGAAAATACAAAGCAAATTATACTTACCAACCCTTCATTGGGAGATACAAAAACTTCAAACTACAAGGTTGTACCACTGATTCCACCTCCTCATGCCGTCCGCCTTCTGTCTCACCTCCCGGCTATGGCGGCAAGCTGAGCCGTCAGCAAAATGTTGCAATGCAATGGGTCCAAAATAATTACTTGGTCTATGATTATTGTCATGATCCTAAGAGAGACCATGCACTTACTCCAGAATGCTAA
Protein sequence

>MS.gene31221.t1

MAPLFSLFFSFMICISNAGGAPSPGYYPSSKISTISFDQEFRNLWGAQHQKLDHESLSIWLDSTSGSGFKSYHSYKSGYFGTAVKLQPGYTAGVNTNLYLSNIEDHKGNHDEIDIEFLGTTQDKPYVLQTNVYIRGSGDGKIIGREMRFHLWFDPTQDFHNYAILWKPSEIIFLVDDVPIRRYPRKSDATFPSRPMYVYGSIWDASAWATENGKYKANYTYQPFIGRYKNFKLQGCTTDSTSSCRPPSVSPPGYGGKLSRQQNVAMQWVQNNYLVYDYCHDPKRDHALTPEC