AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43303

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43303.t1 MTR_2g095800 95.730 281 12 0 1 281 29 309 0.0 570
MS.gene43303.t1 MTR_4g057450 80.505 277 53 1 5 281 29 304 1.26e-169 471
MS.gene43303.t1 MTR_1g063020 67.972 281 89 1 1 281 15 294 7.91e-145 408
MS.gene43303.t1 MTR_7g102260 68.327 281 88 1 1 281 12 291 3.19e-144 407
MS.gene43303.t1 MTR_1g033420 42.642 265 141 5 24 281 31 291 1.01e-67 213
MS.gene43303.t1 MTR_8g064180 42.222 270 141 7 24 281 44 310 6.42e-64 203
MS.gene43303.t1 MTR_6g088320 39.179 268 143 7 25 281 25 283 4.59e-61 195
MS.gene43303.t1 MTR_4g126920 39.850 266 143 6 25 281 24 281 3.21e-59 190
MS.gene43303.t1 MTR_5g029100 37.132 272 157 5 21 281 37 305 6.91e-59 191
MS.gene43303.t1 MTR_3g089540 41.418 268 132 8 25 281 29 282 1.15e-58 189
MS.gene43303.t1 MTR_5g091730 39.326 267 137 8 25 281 27 278 6.94e-58 187
MS.gene43303.t1 MTR_8g090100 37.943 282 148 7 16 281 11 281 7.24e-58 188
MS.gene43303.t1 MTR_4g045853 37.895 285 142 7 18 281 18 288 3.79e-57 185
MS.gene43303.t1 MTR_4g128410 40.377 265 142 8 25 281 24 280 8.34e-57 184
MS.gene43303.t1 MTR_7g084770 36.552 290 164 7 2 281 8 287 1.07e-56 184
MS.gene43303.t1 MTR_2g089140 36.364 275 153 5 21 281 20 286 3.04e-56 183
MS.gene43303.t1 MTR_1g105495 37.591 274 153 7 19 281 28 294 3.29e-56 183
MS.gene43303.t1 MTR_7g093530 39.636 275 144 7 21 281 13 279 3.51e-56 182
MS.gene43303.t1 MTR_3g084620 38.971 272 146 7 18 281 18 277 9.27e-56 181
MS.gene43303.t1 MTR_3g084630 38.971 272 146 7 18 281 18 277 9.78e-56 181
MS.gene43303.t1 MTR_5g029100 38.983 236 132 4 55 281 42 274 5.26e-55 180
MS.gene43303.t1 MTR_7g084760 37.687 268 147 7 24 281 30 287 6.56e-55 179
MS.gene43303.t1 MTR_8g013055 39.552 268 141 7 24 281 29 285 1.06e-54 179
MS.gene43303.t1 MTR_8g081040 40.074 272 144 7 18 281 21 281 1.17e-54 178
MS.gene43303.t1 MTR_4g128570 39.924 263 132 8 25 281 30 272 1.19e-54 178
MS.gene43303.t1 MTR_7g084750 35.517 290 164 9 1 281 12 287 1.45e-54 179
MS.gene43303.t1 MTR_2g038860 38.519 270 145 6 25 281 25 286 6.74e-54 177
MS.gene43303.t1 MTR_2g038705 38.519 270 145 6 25 281 25 286 6.74e-54 177
MS.gene43303.t1 MTR_8g102910 37.079 267 150 6 25 281 21 279 1.74e-53 176
MS.gene43303.t1 MTR_4g128580 38.662 269 145 6 25 281 25 285 1.87e-53 176
MS.gene43303.t1 MTR_4g097640 35.739 291 153 10 2 281 20 287 2.21e-53 175
MS.gene43303.t1 MTR_4g097670 35.739 291 153 10 2 281 20 287 2.21e-53 175
MS.gene43303.t1 MTR_4g097700 37.313 268 146 9 25 281 68 324 4.09e-53 176
MS.gene43303.t1 MTR_6g033085 36.567 268 150 8 24 281 30 287 4.64e-53 174
MS.gene43303.t1 MTR_1g105900 35.766 274 158 8 18 281 26 291 4.86e-52 172
MS.gene43303.t1 MTR_6g033055 35.821 268 152 8 24 281 30 287 2.29e-51 170
MS.gene43303.t1 MTR_4g128400 37.891 256 143 6 34 281 36 283 3.13e-51 170
MS.gene43303.t1 MTR_8g102220 37.500 272 138 9 18 281 19 266 2.67e-49 164
MS.gene43303.t1 MTR_7g056090 38.148 270 147 10 19 281 25 281 4.58e-49 164
MS.gene43303.t1 MTR_2g089140 37.991 229 120 5 67 281 3 223 2.25e-48 160
MS.gene43303.t1 MTR_4g128590 36.531 271 133 9 25 281 30 275 1.41e-47 160
MS.gene43303.t1 MTR_2g049130 33.465 254 150 8 23 268 26 268 3.18e-44 151
MS.gene43303.t1 MTR_2g049030 33.333 270 153 12 21 281 25 276 2.96e-42 146
MS.gene43303.t1 MTR_8g032640 33.333 180 107 5 45 222 32 200 3.50e-18 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43303.t1 AT2G36870 69.203 276 84 1 6 281 23 297 2.18e-143 405
MS.gene43303.t1 AT3G44990 67.647 272 87 1 10 281 23 293 5.56e-137 388
MS.gene43303.t1 AT2G36870 71.154 156 45 0 6 161 23 178 8.26e-79 236
MS.gene43303.t1 AT1G14720 39.927 273 154 4 16 281 21 290 5.32e-69 217
MS.gene43303.t1 AT1G32170 40.074 272 151 4 19 281 25 293 1.10e-65 209
MS.gene43303.t1 AT2G01850 40.977 266 143 5 25 281 30 290 9.03e-65 206
MS.gene43303.t1 AT1G10550 35.971 278 161 3 18 281 35 309 1.06e-62 200
MS.gene43303.t1 AT4G18990 38.652 282 148 5 21 281 35 312 4.16e-62 200
MS.gene43303.t1 AT3G25050 37.736 265 150 4 24 281 33 289 2.24e-61 196
MS.gene43303.t1 AT3G23730 40.892 269 139 8 25 281 26 286 2.11e-60 193
MS.gene43303.t1 AT4G14130 41.791 268 135 8 25 281 27 284 3.81e-60 193
MS.gene43303.t1 AT5G57550 40.784 255 135 7 25 273 29 273 1.68e-59 191
MS.gene43303.t1 AT1G11545 39.927 273 142 8 23 281 35 299 4.38e-59 191
MS.gene43303.t1 AT4G03210 37.455 275 144 6 21 281 24 284 1.56e-58 189
MS.gene43303.t1 AT5G57560 39.552 268 143 8 25 281 22 281 6.33e-58 187
MS.gene43303.t1 AT5G57530 40.809 272 139 9 21 281 22 282 6.27e-57 184
MS.gene43303.t1 AT4G13090 39.925 268 144 6 23 281 29 288 9.80e-57 184
MS.gene43303.t1 AT4G25810 40.221 271 140 10 23 281 23 283 1.65e-56 183
MS.gene43303.t1 AT4G13080 38.577 267 147 8 22 281 35 291 7.11e-55 179
MS.gene43303.t1 AT4G37800 38.868 265 144 6 26 281 34 289 8.16e-55 179
MS.gene43303.t1 AT5G57540 41.045 268 136 9 25 281 25 281 1.12e-54 179
MS.gene43303.t1 AT4G30270 37.984 258 135 7 21 273 19 256 1.68e-54 177
MS.gene43303.t1 AT5G65730 38.258 264 149 6 25 281 34 290 2.18e-54 178
MS.gene43303.t1 AT5G48070 39.474 266 140 10 25 281 28 281 5.88e-54 177
MS.gene43303.t1 AT5G13870 35.294 289 169 7 2 281 8 287 2.24e-53 176
MS.gene43303.t1 AT5G13870 35.294 289 169 7 2 281 8 287 2.24e-53 176
MS.gene43303.t1 AT5G13870 35.294 289 169 7 2 281 8 287 2.24e-53 176
MS.gene43303.t1 AT2G06850 36.861 274 155 7 17 281 26 290 3.59e-53 175
MS.gene43303.t1 AT4G28850 35.075 268 153 8 26 281 29 287 2.02e-52 173
MS.gene43303.t1 AT1G65310 40.000 265 140 8 25 281 28 281 2.09e-52 172
MS.gene43303.t1 AT1G65310 40.000 265 140 8 25 281 50 303 3.39e-52 173
MS.gene43303.t1 AT4G03210 38.333 240 120 6 56 281 31 256 6.48e-52 171
MS.gene43303.t1 AT2G18800 36.462 277 147 9 26 281 28 296 9.35e-52 172
MS.gene43303.t1 AT4G25820 39.852 271 141 9 22 281 26 285 1.54e-51 171
MS.gene43303.t1 AT4G30290 40.693 231 123 6 55 281 56 276 2.49e-51 170
MS.gene43303.t1 AT4G30280 39.216 255 139 5 25 273 28 272 4.28e-50 167
MS.gene43303.t1 AT2G06850 38.723 235 128 6 54 281 5 230 6.16e-50 165
MS.gene43303.t1 AT2G14620 35.401 274 159 8 18 281 29 294 1.03e-49 166
MS.gene43303.t1 AT4G18990 39.631 217 108 4 84 281 26 238 1.35e-48 163
MS.gene43303.t1 AT2G14620 36.596 235 133 7 55 281 18 244 2.05e-45 154
MS.gene43303.t1 AT2G14620 36.564 227 128 7 63 281 15 233 3.99e-43 147
MS.gene43303.t1 AT3G48580 33.206 262 151 8 24 281 32 273 1.92e-40 142
MS.gene43303.t1 AT3G48580 34.199 231 129 7 54 281 4 214 2.76e-37 132
MS.gene43303.t1 AT3G48580 29.389 262 141 9 24 281 32 253 5.70e-25 100

Find 72 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTACAAATCTGGATATTT+TGG 0.149428 2.1:-7852556 MS.gene43303:CDS
CATAGACCAAGTAATTATTT+TGG 0.207651 2.1:+7851067 None:intergenic
TTCCTAGGATACCTCCTTAT+TGG 0.217615 2.1:+7851339 None:intergenic
ATCAAGAATTTAGAAATCTA+TGG 0.228439 2.1:-7853082 MS.gene43303:CDS
GAGAGATGAGATTTCATCTT+TGG 0.246832 2.1:-7852251 MS.gene43303:CDS
TTCACAACTATGCCATTCTA+TGG 0.276482 2.1:-7852209 MS.gene43303:CDS
CGAACTAAACCTTAGCATTC+TGG 0.321947 2.1:+7851006 None:intergenic
AGTGGAGATGGGAAGATCAT+AGG 0.350619 2.1:-7852276 MS.gene43303:CDS
TATACTTACCAACCCTTCAT+TGG 0.351915 2.1:-7851219 MS.gene43303:CDS
GTAAGTGCATGGTCTCTCTT+AGG 0.376321 2.1:+7851030 None:intergenic
GCAATACATATGGCTTATCT+TGG 0.380400 2.1:+7852322 None:intergenic
TCATATCACTCTTACAAATC+TGG 0.381593 2.1:-7852565 MS.gene43303:CDS
ATGATTTGTATTAGTAATGC+TGG 0.382697 2.1:-7853161 MS.gene43303:CDS
TAGTGGATGATGTTCCAATA+AGG 0.386035 2.1:-7851353 MS.gene43303:CDS
GCTCAGCTTGCCGCCATAGC+CGG 0.389428 2.1:+7851119 None:intergenic
CAGACAAATGTGTACATTAG+AGG 0.390267 2.1:-7852300 MS.gene43303:CDS
ACATTTGTCTGCAATACATA+TGG 0.397108 2.1:+7852312 None:intergenic
CTCAGCTTGCCGCCATAGCC+GGG 0.408183 2.1:+7851120 None:intergenic
ACCATGAATCATTATCAATC+TGG 0.433994 2.1:-7853037 MS.gene43303:CDS
GCCAGATTGATAATGATTCA+TGG 0.436574 2.1:+7853036 None:intergenic
ATACTTACCAACCCTTCATT+GGG 0.448591 2.1:-7851218 MS.gene43303:CDS
CTCACTAGGTTTCCATAGAA+TGG 0.448681 2.1:+7852197 None:intergenic
TGATTTGTATTAGTAATGCT+GGG 0.449518 2.1:-7853160 MS.gene43303:CDS
TCTGTCTCACCTCCCGGCTA+TGG 0.450760 2.1:-7851132 MS.gene43303:CDS
TCAAGACCAATGTATGTGTA+TGG 0.458346 2.1:-7851297 MS.gene43303:CDS
ACATTAGAGGAAGTGGAGAT+GGG 0.459120 2.1:-7852287 MS.gene43303:CDS
AGCAAAATGTTGCAATGCAA+TGG 0.460888 2.1:-7851092 MS.gene43303:CDS
TACACATACATTGGTCTTGA+AGG 0.461246 2.1:+7851300 None:intergenic
TCTGCATGGGCTACAGAGAA+TGG 0.467528 2.1:-7851258 MS.gene43303:CDS
TCAAGAATTTAGAAATCTAT+GGG 0.469409 2.1:-7853081 MS.gene43303:CDS
TACATTAGAGGAAGTGGAGA+TGG 0.475103 2.1:-7852288 MS.gene43303:CDS
AATGTTGCATCACTCTTCCT+AGG 0.480500 2.1:+7851324 None:intergenic
AATGTGTACATTAGAGGAAG+TGG 0.491430 2.1:-7852294 MS.gene43303:CDS
AGGCGGACGGCATGAGGAGG+TGG 0.495521 2.1:+7851155 None:intergenic
GAAATAGACATTGAGTTCCT+TGG 0.499774 2.1:-7852351 MS.gene43303:CDS
ATCTGGCTTGACTCTACCTC+AGG 0.501094 2.1:-7853020 MS.gene43303:intron
ACTCAATGTCTATTTCATCA+TGG 0.503835 2.1:+7852358 None:intergenic
GCAAAATGTTGCAATGCAAT+GGG 0.506732 2.1:-7851091 MS.gene43303:CDS
CAATACATATGGCTTATCTT+GGG 0.512542 2.1:+7852323 None:intergenic
TTCCAATAAGGAGGTATCCT+AGG 0.523994 2.1:-7851341 MS.gene43303:CDS
CCGCCTTCTGTCTCACCTCC+CGG 0.526126 2.1:-7851138 MS.gene43303:CDS
TGTATCTCCCAATGAAGGGT+TGG 0.527457 2.1:+7851211 None:intergenic
CAATCTGGGATGCATCTGCA+TGG 0.536057 2.1:-7851272 MS.gene43303:CDS
ATGGGTCCAAAATAATTACT+TGG 0.539014 2.1:-7851073 MS.gene43303:CDS
TGTATGTGTATGGATCAATC+TGG 0.542899 2.1:-7851287 MS.gene43303:CDS
CTTCAACCAGGTTATACTGC+AGG 0.542998 2.1:-7852520 MS.gene43303:CDS
ACACATACATTGGTCTTGAA+GGG 0.561915 2.1:+7851301 None:intergenic
AAATATTACATGATCTCACT+AGG 0.565995 2.1:+7852183 None:intergenic
GCACTTACTCCAGAATGCTA+AGG 0.580492 2.1:-7851015 None:intergenic
GTCTCACCTCCCGGCTATGG+CGG 0.581515 2.1:-7851129 MS.gene43303:CDS
GACAGAAGGCGGACGGCATG+AGG 0.589748 2.1:+7851149 None:intergenic
CTAGGATAGTACCCTGGTGA+AGG 0.596166 2.1:+7853131 None:intergenic
AGAAGGCGGACGGCATGAGG+AGG 0.599752 2.1:+7851152 None:intergenic
ATTGATCCATACACATACAT+TGG 0.600084 2.1:+7851291 None:intergenic
TTGACTCCTGCAGTATAACC+TGG 0.602424 2.1:+7852514 None:intergenic
TAGTTGTGAAAATCTTGTGT+TGG 0.603177 2.1:+7852222 None:intergenic
TAGCCGGGAGGTGAGACAGA+AGG 0.606757 2.1:+7851135 None:intergenic
AATCTGGGATGCATCTGCAT+GGG 0.616394 2.1:-7851271 MS.gene43303:CDS
GCATGAGGAGGTGGAATCAG+TGG 0.618556 2.1:+7851164 None:intergenic
AGCATTCTGGAGTAAGTGCA+TGG 0.624643 2.1:+7851019 None:intergenic
AGCTTGCCGCCATAGCCGGG+AGG 0.627847 2.1:+7851123 None:intergenic
GATTTGTATTAGTAATGCTG+GGG 0.636926 2.1:-7853159 MS.gene43303:CDS
GTATGTGTATGGATCAATCT+GGG 0.642889 2.1:-7851286 MS.gene43303:CDS
TGGATGATGTTCCAATAAGG+AGG 0.647078 2.1:-7851350 MS.gene43303:CDS
GAGGTGAGACAGAAGGCGGA+CGG 0.648616 2.1:+7851142 None:intergenic
ACTGCGGTTAAGCTTCAACC+AGG 0.650106 2.1:-7852532 MS.gene43303:CDS
GCTTATCTTGGGTAGTACCA+AGG 0.651953 2.1:+7852334 None:intergenic
CCGGGAGGTGAGACAGAAGG+CGG 0.677362 2.1:+7851138 None:intergenic
TTAGAACTAGGATAGTACCC+TGG 0.696164 2.1:+7853125 None:intergenic
CAAGAATTTAGAAATCTATG+GGG 0.710687 2.1:-7853080 MS.gene43303:CDS
TCAAACATCGAAGATCACAA+AGG 0.711833 2.1:-7852384 MS.gene43303:CDS
ATTTGTATTAGTAATGCTGG+GGG 0.723446 2.1:-7853158 MS.gene43303:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACATTAATTCAAATTATA+AGG + chr2.1:7851713-7851732 None:intergenic 10.0%
!! ATGAGTATATATATTATAAA+AGG + chr2.1:7852708-7852727 None:intergenic 10.0%
!! TGAGTATATATATTATAAAA+GGG + chr2.1:7852707-7852726 None:intergenic 10.0%
!!! AATCTATTATTATTTTAGTA+TGG - chr2.1:7851540-7851559 MS.gene43303:intron 10.0%
!!! ATGTTTTAAAATAGTAAAAT+TGG + chr2.1:7852248-7852267 None:intergenic 10.0%
!!! ATTTTTAATAAAAACTGTTT+TGG + chr2.1:7852159-7852178 None:intergenic 10.0%
!!! TTTAAAATATATTATTTCTG+AGG + chr2.1:7852416-7852435 None:intergenic 10.0%
!! AATAAATAAAGAATCACATT+TGG + chr2.1:7851377-7851396 None:intergenic 15.0%
!! TGTTGATATTAATTTCATTT+GGG - chr2.1:7851760-7851779 MS.gene43303:intron 15.0%
!! TTTCTCAAATTATATTAGAT+AGG + chr2.1:7851475-7851494 None:intergenic 15.0%
!!! ATAAAAATGATCTTTTTGAT+AGG + chr2.1:7852666-7852685 None:intergenic 15.0%
!!! TTATATATACAGATTTTTAG+TGG - chr2.1:7852808-7852827 MS.gene43303:intron 15.0%
!!! TTGATCTTTTTATTTGTATT+CGG + chr2.1:7852094-7852113 None:intergenic 15.0%
!!! TTTAGAATATATTATTACTG+AGG + chr2.1:7852457-7852476 None:intergenic 15.0%
!! AAATAAACTAAAATCTCAAG+CGG - chr2.1:7851411-7851430 MS.gene43303:intron 20.0%
!! ATCAAGAATTTAGAAATCTA+TGG - chr2.1:7851096-7851115 MS.gene43303:CDS 20.0%
!! TCAAGAATTTAGAAATCTAT+GGG - chr2.1:7851097-7851116 MS.gene43303:CDS 20.0%
!! TCAATACAAATTTATATGTG+AGG - chr2.1:7851683-7851702 MS.gene43303:intron 20.0%
!! TCAATATTCTATGTTTAGTA+AGG - chr2.1:7852500-7852519 MS.gene43303:intron 20.0%
!!! GTGTTGATATTAATTTCATT+TGG - chr2.1:7851759-7851778 MS.gene43303:intron 20.0%
!!! GTTAAATATTTTATTCCAAG+AGG - chr2.1:7852576-7852595 MS.gene43303:CDS 20.0%
! AAATATTACATGATCTCACT+AGG + chr2.1:7851998-7852017 None:intergenic 25.0%
! ATTTCTCTATACCTATTAGT+AGG + chr2.1:7852625-7852644 None:intergenic 25.0%
! CAAGAATTTAGAAATCTATG+GGG - chr2.1:7851098-7851117 MS.gene43303:CDS 25.0%
! TACAAAAACTTCAAACTACA+AGG - chr2.1:7852986-7853005 MS.gene43303:intron 25.0%
! TATATTATTACTGAGGATCT+TGG + chr2.1:7852450-7852469 None:intergenic 25.0%
! TTATGTATGACACTTCATTT+AGG + chr2.1:7851223-7851242 None:intergenic 25.0%
! TTTCTCTATACCTATTAGTA+GGG + chr2.1:7852624-7852643 None:intergenic 25.0%
!! ATGATTTGTATTAGTAATGC+TGG - chr2.1:7851017-7851036 MS.gene43303:CDS 25.0%
!! CATAGACCAAGTAATTATTT+TGG + chr2.1:7853114-7853133 None:intergenic 25.0%
!! TCTTACAAATCTGGATATTT+TGG - chr2.1:7851622-7851641 MS.gene43303:intron 25.0%
!! TGATTTGTATTAGTAATGCT+GGG - chr2.1:7851018-7851037 MS.gene43303:CDS 25.0%
!! TTACTAATTTTCAGTTCGAT+TGG - chr2.1:7852331-7852350 MS.gene43303:CDS 25.0%
!!! ATAGTGCTTATTTTAGAACT+AGG + chr2.1:7851068-7851087 None:intergenic 25.0%
!!! TATATTATTTCTGAGGATCT+TGG + chr2.1:7852409-7852428 None:intergenic 25.0%
!!! TTTTATTTAAAAGCCACATG+TGG - chr2.1:7852680-7852699 MS.gene43303:intron 25.0%
ACATTTGTCTGCAATACATA+TGG + chr2.1:7851869-7851888 None:intergenic 30.0%
ACCATGAATCATTATCAATC+TGG - chr2.1:7851141-7851160 MS.gene43303:CDS 30.0%
ACTCAATGTCTATTTCATCA+TGG + chr2.1:7851823-7851842 None:intergenic 30.0%
ATGGGTCCAAAATAATTACT+TGG - chr2.1:7853105-7853124 MS.gene43303:CDS 30.0%
ATTGATCCATACACATACAT+TGG + chr2.1:7852890-7852909 None:intergenic 30.0%
ATTTGTATTAGTAATGCTGG+GGG - chr2.1:7851020-7851039 MS.gene43303:CDS 30.0%
CAAATTGAACTGTGATATGT+TGG + chr2.1:7852055-7852074 None:intergenic 30.0%
CAATACATATGGCTTATCTT+GGG + chr2.1:7851858-7851877 None:intergenic 30.0%
CTAATAGGTATAGAGAAATG+AGG - chr2.1:7852626-7852645 MS.gene43303:intron 30.0%
GATTTGTATTAGTAATGCTG+GGG - chr2.1:7851019-7851038 MS.gene43303:CDS 30.0%
GCAAAAGTAAACAAAGAACT+AGG - chr2.1:7852290-7852309 MS.gene43303:CDS 30.0%
TAGTTGTGAAAATCTTGTGT+TGG + chr2.1:7851959-7851978 None:intergenic 30.0%
TCATATCACTCTTACAAATC+TGG - chr2.1:7851613-7851632 MS.gene43303:intron 30.0%
! ATTTTCCATACCCTACTAAT+AGG - chr2.1:7852611-7852630 MS.gene43303:intron 30.0%
! CAGTTCAATTTGTTCAACTA+CGG - chr2.1:7852063-7852082 MS.gene43303:intron 30.0%
! CATTTGATTTCATATACCTG+AGG + chr2.1:7851177-7851196 None:intergenic 30.0%
! GTGATTGATTGATAGCTATA+TGG + chr2.1:7852550-7852569 None:intergenic 30.0%
! TTGATAGCTATATGGCTAAA+TGG + chr2.1:7852542-7852561 None:intergenic 30.0%
! TTTTTGTATCTCCCAATGAA+GGG + chr2.1:7852974-7852993 None:intergenic 30.0%
AAAACTAGACTGAGTCTGAA+AGG + chr2.1:7852371-7852390 None:intergenic 35.0%
AAGCATTTCTTTGCAACACA+GGG - chr2.1:7851578-7851597 MS.gene43303:intron 35.0%
AATGTGTACATTAGAGGAAG+TGG - chr2.1:7851884-7851903 MS.gene43303:intron 35.0%
AATTAAACACTCTTGCCTCT+TGG + chr2.1:7852594-7852613 None:intergenic 35.0%
ACACTTCATTTAGGTGAACT+TGG + chr2.1:7851214-7851233 None:intergenic 35.0%
AGCAAAATGTTGCAATGCAA+TGG - chr2.1:7853086-7853105 MS.gene43303:CDS 35.0%
ATACTTACCAACCCTTCATT+GGG - chr2.1:7852960-7852979 MS.gene43303:intron 35.0%
ATTATAAAAGGGACCACATG+TGG + chr2.1:7852696-7852715 None:intergenic 35.0%
CACTTCATTTAGGTGAACTT+GGG + chr2.1:7851213-7851232 None:intergenic 35.0%
CAGACAAATGTGTACATTAG+AGG - chr2.1:7851878-7851897 MS.gene43303:intron 35.0%
CTATACCTATTAGTAGGGTA+TGG + chr2.1:7852619-7852638 None:intergenic 35.0%
GAAATAGACATTGAGTTCCT+TGG - chr2.1:7851827-7851846 MS.gene43303:intron 35.0%
GAGAGATGAGATTTCATCTT+TGG - chr2.1:7851927-7851946 MS.gene43303:intron 35.0%
GCAAAATGTTGCAATGCAAT+GGG - chr2.1:7853087-7853106 MS.gene43303:CDS 35.0%
GCAATACATATGGCTTATCT+TGG + chr2.1:7851859-7851878 None:intergenic 35.0%
GCTATCAATCAATCACTGTT+TGG - chr2.1:7852554-7852573 MS.gene43303:CDS 35.0%
GTATGTGTATGGATCAATCT+GGG - chr2.1:7852892-7852911 MS.gene43303:intron 35.0%
TAAGCATTTCTTTGCAACAC+AGG - chr2.1:7851577-7851596 MS.gene43303:intron 35.0%
TATACTTACCAACCCTTCAT+TGG - chr2.1:7852959-7852978 MS.gene43303:intron 35.0%
TCAAACATCGAAGATCACAA+AGG - chr2.1:7851794-7851813 MS.gene43303:intron 35.0%
TCAAGACCAATGTATGTGTA+TGG - chr2.1:7852881-7852900 MS.gene43303:intron 35.0%
TGTATGTGTATGGATCAATC+TGG - chr2.1:7852891-7852910 MS.gene43303:intron 35.0%
TTCACAACTATGCCATTCTA+TGG - chr2.1:7851969-7851988 MS.gene43303:intron 35.0%
! GTTTTTGTATCTCCCAATGA+AGG + chr2.1:7852975-7852994 None:intergenic 35.0%
! TAGTGGATGATGTTCCAATA+AGG - chr2.1:7852825-7852844 MS.gene43303:intron 35.0%
!! ACACATACATTGGTCTTGAA+GGG + chr2.1:7852880-7852899 None:intergenic 35.0%
!! GCCAGATTGATAATGATTCA+TGG + chr2.1:7851145-7851164 None:intergenic 35.0%
!! TACACATACATTGGTCTTGA+AGG + chr2.1:7852881-7852900 None:intergenic 35.0%
!!! ATCTGGATATTTTGGTACTG+CGG - chr2.1:7851630-7851649 MS.gene43303:intron 35.0%
AATGTTGCATCACTCTTCCT+AGG + chr2.1:7852857-7852876 None:intergenic 40.0%
ACAAACCTGTTAAACCAACC+TGG - chr2.1:7852179-7852198 MS.gene43303:intron 40.0%
ACATTAGAGGAAGTGGAGAT+GGG - chr2.1:7851891-7851910 MS.gene43303:intron 40.0%
CTCACTAGGTTTCCATAGAA+TGG + chr2.1:7851984-7852003 None:intergenic 40.0%
TAAACCAACCTGGTTGAGAA+TGG - chr2.1:7852189-7852208 MS.gene43303:intron 40.0%
TACATTAGAGGAAGTGGAGA+TGG - chr2.1:7851890-7851909 MS.gene43303:intron 40.0%
TAGCGTGCTGAATTGCTTTA+TGG - chr2.1:7852784-7852803 MS.gene43303:intron 40.0%
TGGATGATGTTCCAATAAGG+AGG - chr2.1:7852828-7852847 MS.gene43303:intron 40.0%
TTAGAACTAGGATAGTACCC+TGG + chr2.1:7851056-7851075 None:intergenic 40.0%
TTCCTAGGATACCTCCTTAT+TGG + chr2.1:7852842-7852861 None:intergenic 40.0%
! GCATTGCAACATTTTGCTGA+CGG + chr2.1:7853084-7853103 None:intergenic 40.0%
!! TTCCAATAAGGAGGTATCCT+AGG - chr2.1:7852837-7852856 MS.gene43303:intron 40.0%
AGTGGAGATGGGAAGATCAT+AGG - chr2.1:7851902-7851921 MS.gene43303:intron 45.0%
CATCAGGCTACATTAGAACG+AGG - chr2.1:7852215-7852234 MS.gene43303:CDS 45.0%
CTCGTTCTAATGTAGCCTGA+TGG + chr2.1:7852217-7852236 None:intergenic 45.0%
CTGAGTCTGAAAGGATCGAT+CGG + chr2.1:7852362-7852381 None:intergenic 45.0%
CTTCAACCAGGTTATACTGC+AGG - chr2.1:7851658-7851677 MS.gene43303:intron 45.0%
GCTTATCTTGGGTAGTACCA+AGG + chr2.1:7851847-7851866 None:intergenic 45.0%
GTAAGTGCATGGTCTCTCTT+AGG + chr2.1:7853151-7853170 None:intergenic 45.0%
TGATGGAACCATTCTCAACC+AGG + chr2.1:7852200-7852219 None:intergenic 45.0%
TGGTTGAGAATGGTTCCATC+AGG - chr2.1:7852199-7852218 MS.gene43303:CDS 45.0%
TGTATCTCCCAATGAAGGGT+TGG + chr2.1:7852970-7852989 None:intergenic 45.0%
TTGACTCCTGCAGTATAACC+TGG + chr2.1:7851667-7851686 None:intergenic 45.0%
TTTCTTTGCAACACAGGGAG+TGG - chr2.1:7851583-7851602 MS.gene43303:intron 45.0%
! AATCTGGGATGCATCTGCAT+GGG - chr2.1:7852907-7852926 MS.gene43303:intron 45.0%
!! AGCATTCTGGAGTAAGTGCA+TGG + chr2.1:7853162-7853181 None:intergenic 45.0%
!! CTCAACCAGGTTGGTTTAAC+AGG + chr2.1:7852187-7852206 None:intergenic 45.0%
ACTGCGGTTAAGCTTCAACC+AGG - chr2.1:7851646-7851665 MS.gene43303:intron 50.0%
CTAGGATAGTACCCTGGTGA+AGG + chr2.1:7851050-7851069 None:intergenic 50.0%
GGAACCATTCTCAACCAGGT+TGG + chr2.1:7852196-7852215 None:intergenic 50.0%
! ATCTGGCTTGACTCTACCTC+AGG - chr2.1:7851158-7851177 MS.gene43303:CDS 50.0%
! CAATCTGGGATGCATCTGCA+TGG - chr2.1:7852906-7852925 MS.gene43303:intron 50.0%
! TCTGCATGGGCTACAGAGAA+TGG - chr2.1:7852920-7852939 MS.gene43303:intron 50.0%
GCATGAGGAGGTGGAATCAG+TGG + chr2.1:7853017-7853036 None:intergenic 55.0%
GAGGTGAGACAGAAGGCGGA+CGG + chr2.1:7853039-7853058 None:intergenic 60.0%
TAGCCGGGAGGTGAGACAGA+AGG + chr2.1:7853046-7853065 None:intergenic 60.0%
TCTGTCTCACCTCCCGGCTA+TGG - chr2.1:7853046-7853065 MS.gene43303:CDS 60.0%
AGAAGGCGGACGGCATGAGG+AGG + chr2.1:7853029-7853048 None:intergenic 65.0%
CCGCCTTCTGTCTCACCTCC+CGG - chr2.1:7853040-7853059 MS.gene43303:CDS 65.0%
CCGGGAGGTGAGACAGAAGG+CGG + chr2.1:7853043-7853062 None:intergenic 65.0%
CTCAGCTTGCCGCCATAGCC+GGG + chr2.1:7853061-7853080 None:intergenic 65.0%
GACAGAAGGCGGACGGCATG+AGG + chr2.1:7853032-7853051 None:intergenic 65.0%
GCTCAGCTTGCCGCCATAGC+CGG + chr2.1:7853062-7853081 None:intergenic 65.0%
GTCTCACCTCCCGGCTATGG+CGG - chr2.1:7853049-7853068 MS.gene43303:CDS 65.0%
! CTGGGGGTGCACCTTCACCA+GGG - chr2.1:7851036-7851055 MS.gene43303:CDS 65.0%
AGCTTGCCGCCATAGCCGGG+AGG + chr2.1:7853058-7853077 None:intergenic 70.0%
AGGCGGACGGCATGAGGAGG+TGG + chr2.1:7853026-7853045 None:intergenic 70.0%
! GCTGGGGGTGCACCTTCACC+AGG - chr2.1:7851035-7851054 MS.gene43303:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 7851017 7853183 7851017 ID=MS.gene43303
chr2.1 mRNA 7851017 7853183 7851017 ID=MS.gene43303.t1;Parent=MS.gene43303
chr2.1 exon 7853021 7853183 7853021 ID=MS.gene43303.t1.exon1;Parent=MS.gene43303.t1
chr2.1 CDS 7853021 7853183 7853021 ID=cds.MS.gene43303.t1;Parent=MS.gene43303.t1
chr2.1 exon 7852501 7852601 7852501 ID=MS.gene43303.t1.exon2;Parent=MS.gene43303.t1
chr2.1 CDS 7852501 7852601 7852501 ID=cds.MS.gene43303.t1;Parent=MS.gene43303.t1
chr2.1 exon 7852192 7852409 7852192 ID=MS.gene43303.t1.exon3;Parent=MS.gene43303.t1
chr2.1 CDS 7852192 7852409 7852192 ID=cds.MS.gene43303.t1;Parent=MS.gene43303.t1
chr2.1 exon 7851017 7851380 7851017 ID=MS.gene43303.t1.exon4;Parent=MS.gene43303.t1
chr2.1 CDS 7851017 7851380 7851017 ID=cds.MS.gene43303.t1;Parent=MS.gene43303.t1
Gene Sequence

>MS.gene43303

ATGATTTGTATTAGTAATGCTGGGGGTGCACCTTCACCAGGGTACTATCCTAGTTCTAAAATAAGCACTATTAGCTTTGATCAAGAATTTAGAAATCTATGGGGAGCTCAGCATCAGAAACTAGACCATGAATCATTATCAATCTGGCTTGACTCTACCTCAGGTATATGAAATCAAATGTCTCAAATCATCTCCCAAGTTCACCTAAATGAAGTGTCATACATAATCATTAACTCGAAAAATAACTCTTGAAATTTCAAGCATTTCATAATTTGTTGCATGTGTACATAAATTTTGTTACAATTTTAATTTATTTCATCTTTGTACTATATAATACTCTTCTCAATTTTAGATGAGCCAAATGTGATTCTTTATTTATTTGTTATTGTAATCTAAATAAACTAAAATCTCAAGCGGTTAAAACTCTTGATGAAGTACTTTGTCTTACACGTGTTCCTATCTAATATAATTTGAGAAATTAGTCTCTGCGACTACGTACATAGTACATAAAATATAATGCAAAAATCTATTATTATTTTAGTATGGATTCTTATTACTTCTAAGCATTTCTTTGCAACACAGGGAGTGGATTCAAGTCATATCACTCTTACAAATCTGGATATTTTGGTACTGCGGTTAAGCTTCAACCAGGTTATACTGCAGGAGTCAATACAAATTTATATGTGAGGATTACCTTATAATTTGAATTAATGTTTAAAATTGACTGTATATTACGTTTTTTGTGTTGATATTAATTTCATTTGGGAATTGTAGCTTTCAAACATCGAAGATCACAAAGGAAACCATGATGAAATAGACATTGAGTTCCTTGGTACTACCCAAGATAAGCCATATGTATTGCAGACAAATGTGTACATTAGAGGAAGTGGAGATGGGAAGATCATAGGAAGAGAGATGAGATTTCATCTTTGGTTCGATCCAACACAAGATTTTCACAACTATGCCATTCTATGGAAACCTAGTGAGATCATGTAATATTTTCATACACTATTCTACAATGTTTTAAAATCTAGACCAACATATCACAGTTCAATTTGTTCAACTACGGTTAAACCGAATACAAATAAAAAGATCAAAAGTTTTTTTTTTGACAAAAGATCAAAAGTATTTAAAAAGTACCAAAACAGTTTTTATTAAAAATACAAACCTGTTAAACCAACCTGGTTGAGAATGGTTCCATCAGGCTACATTAGAACGAGGATTAAATCCAATTTTACTATTTTAAAACATATTTGAGTTTTTATATTCTAAAGCAAAAGTAAACAAAGAACTAGGATTAATTCGATCATGAAGTTACTAATTTTCAGTTCGATTGGTTGAACCGATCGATCCTTTCAGACTCAGTCTAGTTTTTAAAACATTGTTATACCAAGATCCTCAGAAATAATATATTTTAAAACATTGTTATACCAAGATCCTCAGTAATAATATATTCTAAATATAAATATGACTTTATTTTGTGTCAATATTCTATGTTTAGTAAGGATCAACAAGTCAACAACCATTTAGCCATATAGCTATCAATCAATCACTGTTTGGTTAAATATTTTATTCCAAGAGGCAAGAGTGTTTAATTTTCCATACCCTACTAATAGGTATAGAGAAATGAGGTGCTAGAAAATATTCCTATCAAAAAGATCATTTTTATTTAAAAGCCACATGTGGTCCCTTTTATAATATATATACTCATTAACATTATAATGTCATGTTTATTAAAAAACAAAGCATATAGCTATTTTTTTTTGTTAGCGTGCTGAATTGCTTTATGGTTTATATATACAGATTTTTAGTGGATGATGTTCCAATAAGGAGGTATCCTAGGAAGAGTGATGCAACATTCCCTTCAAGACCAATGTATGTGTATGGATCAATCTGGGATGCATCTGCATGGGCTACAGAGAATGGAAAATACAAAGCAAATTATACTTACCAACCCTTCATTGGGAGATACAAAAACTTCAAACTACAAGGTTGTACCACTGATTCCACCTCCTCATGCCGTCCGCCTTCTGTCTCACCTCCCGGCTATGGCGGCAAGCTGAGCCGTCAGCAAAATGTTGCAATGCAATGGGTCCAAAATAATTACTTGGTCTATGATTATTGTCATGATCCTAAGAGAGACCATGCACTTACTCCAGAATGCTAA
Protein sequence

>MS.gene43303.t1

MICISNAGGAPSPGYYPSSKISTISFDQEFRNLWGAQHQKLDHESLSIWLDSTSGSGFKSYHSYKSGYFGTAVKLQPGYTAGVNTNLYLSNIEDHKGNHDEIDIEFLGTTQDKPYVLQTNVYIRGSGDGKIIGREMRFHLWFDPTQDFHNYAILWKPSEIIFLVDDVPIRRYPRKSDATFPSRPMYVYGSIWDASAWATENGKYKANYTYQPFIGRYKNFKLQGCTTDSTSSCRPPSVSPPGYGGKLSRQQNVAMQWVQNNYLVYDYCHDPKRDHALTPEC