Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042579.t1 | RHN50825.1 | 98 | 293 | 6 | 0 | 1 | 293 | 24 | 316 | 3.00E-174 | 620.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042579.t1 | Q41638 | 90.1 | 293 | 28 | 1 | 1 | 293 | 1 | 292 | 2.9e-162 | 572.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042579.t1 | A0A072U942 | 98.0 | 293 | 6 | 0 | 1 | 293 | 1 | 293 | 2.1e-174 | 620.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene45618 | MS.gene042579 | PPI |
| MS.gene43849 | MS.gene042579 | PPI |
| MS.gene049783 | MS.gene042579 | PPI |
| MS.gene028653 | MS.gene042579 | PPI |
| MS.gene042579 | MS.gene011681 | PPI |
| MS.gene44430 | MS.gene042579 | PPI |
| MS.gene011682 | MS.gene042579 | PPI |
| MS.gene45619 | MS.gene042579 | PPI |
| MS.gene31221 | MS.gene042579 | PPI |
| MS.gene042579 | MS.gene09167 | PPI |
| MS.gene44431 | MS.gene042579 | PPI |
| MS.gene60167 | MS.gene042579 | PPI |
| MS.gene43303 | MS.gene042579 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042579.t1 | MTR_6g033055 | 97.952 | 293 | 6 | 0 | 1 | 293 | 1 | 293 | 0.0 | 604 |
| MS.gene042579.t1 | MTR_6g033085 | 95.563 | 293 | 13 | 0 | 1 | 293 | 1 | 293 | 0.0 | 592 |
| MS.gene042579.t1 | MTR_7g084760 | 84.300 | 293 | 46 | 0 | 1 | 293 | 1 | 293 | 0.0 | 530 |
| MS.gene042579.t1 | MTR_7g084770 | 83.618 | 293 | 48 | 0 | 1 | 293 | 1 | 293 | 0.0 | 528 |
| MS.gene042579.t1 | MTR_7g084750 | 80.546 | 293 | 57 | 0 | 1 | 293 | 1 | 293 | 0.0 | 513 |
| MS.gene042579.t1 | MTR_4g128410 | 56.028 | 282 | 110 | 4 | 9 | 287 | 10 | 280 | 1.58e-106 | 311 |
| MS.gene042579.t1 | MTR_4g097640 | 51.773 | 282 | 129 | 3 | 7 | 287 | 12 | 287 | 4.48e-103 | 303 |
| MS.gene042579.t1 | MTR_4g097670 | 51.773 | 282 | 129 | 3 | 7 | 287 | 12 | 287 | 4.48e-103 | 303 |
| MS.gene042579.t1 | MTR_4g045853 | 48.980 | 294 | 129 | 4 | 4 | 289 | 10 | 290 | 1.59e-102 | 301 |
| MS.gene042579.t1 | MTR_4g097700 | 54.475 | 257 | 115 | 2 | 32 | 287 | 69 | 324 | 5.43e-102 | 301 |
| MS.gene042579.t1 | MTR_3g084620 | 52.069 | 290 | 122 | 4 | 3 | 291 | 8 | 281 | 7.50e-101 | 297 |
| MS.gene042579.t1 | MTR_3g084630 | 51.027 | 292 | 131 | 3 | 1 | 291 | 1 | 281 | 1.37e-100 | 296 |
| MS.gene042579.t1 | MTR_2g089140 | 47.766 | 291 | 135 | 5 | 8 | 289 | 6 | 288 | 3.15e-100 | 295 |
| MS.gene042579.t1 | MTR_7g093530 | 53.546 | 282 | 117 | 4 | 9 | 287 | 9 | 279 | 2.41e-99 | 293 |
| MS.gene042579.t1 | MTR_8g081040 | 52.113 | 284 | 120 | 5 | 7 | 289 | 15 | 283 | 4.54e-99 | 292 |
| MS.gene042579.t1 | MTR_4g128570 | 52.650 | 283 | 115 | 4 | 7 | 288 | 9 | 273 | 1.67e-98 | 290 |
| MS.gene042579.t1 | MTR_4g128580 | 55.263 | 266 | 107 | 3 | 32 | 289 | 26 | 287 | 2.31e-98 | 291 |
| MS.gene042579.t1 | MTR_4g128400 | 50.360 | 278 | 128 | 3 | 11 | 287 | 15 | 283 | 3.04e-94 | 280 |
| MS.gene042579.t1 | MTR_1g105495 | 48.239 | 284 | 143 | 2 | 9 | 288 | 12 | 295 | 3.93e-93 | 278 |
| MS.gene042579.t1 | MTR_2g038860 | 49.658 | 292 | 125 | 5 | 9 | 291 | 12 | 290 | 5.67e-92 | 275 |
| MS.gene042579.t1 | MTR_2g038705 | 49.658 | 292 | 125 | 5 | 9 | 291 | 12 | 290 | 5.67e-92 | 275 |
| MS.gene042579.t1 | MTR_4g126920 | 49.821 | 279 | 123 | 4 | 15 | 288 | 16 | 282 | 1.36e-91 | 273 |
| MS.gene042579.t1 | MTR_8g102220 | 48.973 | 292 | 119 | 6 | 1 | 291 | 8 | 270 | 3.77e-91 | 272 |
| MS.gene042579.t1 | MTR_1g105900 | 47.331 | 281 | 143 | 3 | 10 | 288 | 15 | 292 | 1.22e-90 | 271 |
| MS.gene042579.t1 | MTR_6g088320 | 47.015 | 268 | 136 | 4 | 28 | 291 | 22 | 287 | 2.14e-89 | 268 |
| MS.gene042579.t1 | MTR_8g102910 | 43.771 | 297 | 147 | 6 | 1 | 291 | 1 | 283 | 2.94e-89 | 267 |
| MS.gene042579.t1 | MTR_2g089140 | 52.000 | 225 | 100 | 2 | 73 | 289 | 1 | 225 | 9.09e-89 | 264 |
| MS.gene042579.t1 | MTR_8g013055 | 48.263 | 259 | 133 | 1 | 29 | 287 | 28 | 285 | 6.16e-87 | 261 |
| MS.gene042579.t1 | MTR_4g128590 | 46.441 | 295 | 122 | 5 | 2 | 287 | 8 | 275 | 3.93e-84 | 254 |
| MS.gene042579.t1 | MTR_5g091730 | 43.299 | 291 | 146 | 4 | 2 | 289 | 6 | 280 | 1.24e-77 | 238 |
| MS.gene042579.t1 | MTR_3g089540 | 46.721 | 244 | 125 | 2 | 51 | 293 | 49 | 288 | 1.84e-76 | 235 |
| MS.gene042579.t1 | MTR_7g056090 | 42.857 | 280 | 151 | 6 | 9 | 287 | 10 | 281 | 5.01e-73 | 226 |
| MS.gene042579.t1 | MTR_2g049030 | 43.369 | 279 | 145 | 9 | 11 | 287 | 9 | 276 | 1.08e-69 | 217 |
| MS.gene042579.t1 | MTR_2g049130 | 42.391 | 276 | 149 | 8 | 7 | 277 | 1 | 271 | 3.57e-65 | 206 |
| MS.gene042579.t1 | MTR_8g064180 | 35.452 | 299 | 172 | 5 | 9 | 287 | 13 | 310 | 2.27e-60 | 194 |
| MS.gene042579.t1 | MTR_4g057450 | 35.786 | 299 | 159 | 8 | 9 | 287 | 19 | 304 | 5.44e-59 | 191 |
| MS.gene042579.t1 | MTR_1g033420 | 35.593 | 295 | 175 | 6 | 5 | 290 | 6 | 294 | 7.29e-57 | 186 |
| MS.gene042579.t1 | MTR_5g029100 | 37.546 | 269 | 154 | 6 | 30 | 287 | 40 | 305 | 6.55e-56 | 184 |
| MS.gene042579.t1 | MTR_1g063020 | 35.906 | 298 | 163 | 9 | 9 | 287 | 6 | 294 | 1.21e-55 | 182 |
| MS.gene042579.t1 | MTR_7g102260 | 34.551 | 301 | 169 | 9 | 7 | 288 | 1 | 292 | 2.52e-55 | 181 |
| MS.gene042579.t1 | MTR_8g090100 | 36.667 | 270 | 154 | 6 | 30 | 288 | 19 | 282 | 1.58e-54 | 180 |
| MS.gene042579.t1 | MTR_2g095800 | 36.567 | 268 | 150 | 8 | 30 | 287 | 52 | 309 | 2.81e-53 | 176 |
| MS.gene042579.t1 | MTR_5g029100 | 39.024 | 246 | 135 | 7 | 54 | 287 | 32 | 274 | 4.34e-52 | 173 |
| MS.gene042579.t1 | MTR_8g479340 | 34.759 | 187 | 61 | 3 | 103 | 288 | 10 | 136 | 2.77e-27 | 103 |
| MS.gene042579.t1 | MTR_8g032640 | 31.609 | 174 | 111 | 4 | 54 | 225 | 33 | 200 | 3.96e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042579.t1 | AT5G13870 | 76.109 | 293 | 67 | 1 | 1 | 293 | 4 | 293 | 1.81e-175 | 486 |
| MS.gene042579.t1 | AT5G13870 | 76.109 | 293 | 67 | 1 | 1 | 293 | 4 | 293 | 1.81e-175 | 486 |
| MS.gene042579.t1 | AT5G13870 | 76.109 | 293 | 67 | 1 | 1 | 293 | 4 | 293 | 1.81e-175 | 486 |
| MS.gene042579.t1 | AT2G06850 | 72.973 | 296 | 73 | 2 | 1 | 292 | 3 | 295 | 5.64e-167 | 465 |
| MS.gene042579.t1 | AT2G06850 | 78.788 | 231 | 49 | 0 | 62 | 292 | 5 | 235 | 7.87e-144 | 404 |
| MS.gene042579.t1 | AT5G65730 | 50.877 | 285 | 133 | 3 | 4 | 287 | 12 | 290 | 2.49e-105 | 308 |
| MS.gene042579.t1 | AT3G23730 | 52.281 | 285 | 129 | 3 | 11 | 291 | 9 | 290 | 1.85e-104 | 306 |
| MS.gene042579.t1 | AT4G14130 | 51.903 | 289 | 124 | 4 | 7 | 291 | 11 | 288 | 2.41e-103 | 303 |
| MS.gene042579.t1 | AT5G57550 | 51.748 | 286 | 125 | 3 | 3 | 287 | 9 | 282 | 6.06e-102 | 300 |
| MS.gene042579.t1 | AT5G57560 | 51.579 | 285 | 119 | 4 | 10 | 287 | 9 | 281 | 4.27e-100 | 295 |
| MS.gene042579.t1 | AT4G37800 | 49.141 | 291 | 144 | 3 | 1 | 288 | 1 | 290 | 8.07e-100 | 295 |
| MS.gene042579.t1 | AT4G03210 | 48.110 | 291 | 143 | 3 | 5 | 293 | 6 | 290 | 4.32e-99 | 293 |
| MS.gene042579.t1 | AT4G30270 | 51.761 | 284 | 110 | 5 | 9 | 288 | 6 | 266 | 1.67e-97 | 288 |
| MS.gene042579.t1 | AT4G25810 | 50.871 | 287 | 131 | 4 | 7 | 287 | 1 | 283 | 3.17e-97 | 288 |
| MS.gene042579.t1 | AT4G13090 | 49.822 | 281 | 134 | 4 | 12 | 287 | 10 | 288 | 3.37e-95 | 283 |
| MS.gene042579.t1 | AT5G57530 | 54.941 | 253 | 106 | 4 | 39 | 287 | 34 | 282 | 3.32e-94 | 280 |
| MS.gene042579.t1 | AT5G57540 | 53.846 | 260 | 112 | 4 | 32 | 287 | 26 | 281 | 8.85e-94 | 279 |
| MS.gene042579.t1 | AT1G11545 | 48.951 | 286 | 136 | 4 | 9 | 288 | 19 | 300 | 3.53e-93 | 278 |
| MS.gene042579.t1 | AT4G03210 | 49.609 | 256 | 124 | 2 | 40 | 293 | 10 | 262 | 4.75e-92 | 274 |
| MS.gene042579.t1 | AT2G18800 | 50.183 | 273 | 117 | 4 | 32 | 288 | 28 | 297 | 1.19e-91 | 274 |
| MS.gene042579.t1 | AT4G28850 | 45.263 | 285 | 149 | 5 | 12 | 291 | 9 | 291 | 5.36e-89 | 267 |
| MS.gene042579.t1 | AT2G14620 | 45.455 | 286 | 152 | 3 | 5 | 288 | 12 | 295 | 1.64e-88 | 266 |
| MS.gene042579.t1 | AT4G25820 | 52.174 | 253 | 113 | 3 | 39 | 287 | 37 | 285 | 1.38e-87 | 263 |
| MS.gene042579.t1 | AT3G25050 | 49.042 | 261 | 126 | 4 | 30 | 287 | 33 | 289 | 2.91e-87 | 263 |
| MS.gene042579.t1 | AT5G48070 | 46.831 | 284 | 144 | 4 | 7 | 288 | 4 | 282 | 3.46e-86 | 259 |
| MS.gene042579.t1 | AT4G13080 | 43.197 | 294 | 157 | 4 | 1 | 287 | 1 | 291 | 1.29e-85 | 258 |
| MS.gene042579.t1 | AT1G65310 | 52.863 | 227 | 101 | 3 | 63 | 288 | 61 | 282 | 2.63e-81 | 247 |
| MS.gene042579.t1 | AT1G65310 | 52.863 | 227 | 101 | 3 | 63 | 288 | 83 | 304 | 2.71e-81 | 248 |
| MS.gene042579.t1 | AT2G14620 | 50.439 | 228 | 111 | 2 | 63 | 288 | 18 | 245 | 3.17e-81 | 246 |
| MS.gene042579.t1 | AT4G30280 | 51.982 | 227 | 103 | 3 | 63 | 288 | 61 | 282 | 4.24e-81 | 246 |
| MS.gene042579.t1 | AT4G30290 | 51.867 | 241 | 110 | 3 | 49 | 288 | 42 | 277 | 1.22e-80 | 245 |
| MS.gene042579.t1 | AT2G14620 | 50.909 | 220 | 106 | 2 | 71 | 288 | 15 | 234 | 2.15e-79 | 241 |
| MS.gene042579.t1 | AT3G44990 | 39.456 | 294 | 152 | 10 | 9 | 287 | 11 | 293 | 1.55e-60 | 194 |
| MS.gene042579.t1 | AT1G32170 | 35.117 | 299 | 163 | 6 | 5 | 288 | 12 | 294 | 1.10e-56 | 186 |
| MS.gene042579.t1 | AT3G48580 | 39.768 | 259 | 138 | 6 | 30 | 287 | 32 | 273 | 2.69e-56 | 183 |
| MS.gene042579.t1 | AT1G14720 | 34.708 | 291 | 181 | 6 | 3 | 288 | 5 | 291 | 6.60e-56 | 184 |
| MS.gene042579.t1 | AT1G10550 | 38.340 | 253 | 137 | 6 | 51 | 287 | 60 | 309 | 2.93e-55 | 181 |
| MS.gene042579.t1 | AT2G36870 | 40.417 | 240 | 128 | 5 | 58 | 289 | 67 | 299 | 2.09e-54 | 179 |
| MS.gene042579.t1 | AT2G01850 | 35.154 | 293 | 177 | 8 | 3 | 288 | 5 | 291 | 2.19e-53 | 177 |
| MS.gene042579.t1 | AT4G18990 | 37.008 | 254 | 139 | 5 | 54 | 288 | 62 | 313 | 4.22e-51 | 172 |
| MS.gene042579.t1 | AT3G48580 | 40.088 | 227 | 119 | 5 | 62 | 287 | 4 | 214 | 1.77e-49 | 164 |
| MS.gene042579.t1 | AT3G48580 | 34.363 | 259 | 132 | 7 | 30 | 287 | 32 | 253 | 4.43e-41 | 143 |
| MS.gene042579.t1 | AT4G18990 | 34.978 | 223 | 117 | 5 | 92 | 288 | 19 | 239 | 4.80e-41 | 144 |
| MS.gene042579.t1 | AT2G36870 | 45.536 | 112 | 53 | 2 | 58 | 161 | 67 | 178 | 3.34e-27 | 104 |
Find 61 sgRNAs with CRISPR-Local
Find 145 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAATATACAATAGTTTA+TGG | 0.145009 | 6.3:-21219853 | MS.gene042579:CDS |
| TCCAAAGGGTCTTATTTATT+TGG | 0.166110 | 6.3:-21221269 | MS.gene042579:CDS |
| TAATAAGTACTTACACAATT+TGG | 0.181871 | 6.3:+21220708 | None:intergenic |
| ATGTTCTTGAACACCCTTAT+TGG | 0.186258 | 6.3:+21219914 | None:intergenic |
| GATCATATCAAATACTTAAA+TGG | 0.261028 | 6.3:-21222475 | MS.gene042579:CDS |
| TACAAAGGGTTTCATATTGA+TGG | 0.286250 | 6.3:-21219758 | MS.gene042579:CDS |
| GAGAACAAAGAATCTATCTT+TGG | 0.292863 | 6.3:-21220781 | MS.gene042579:CDS |
| TTGTGGATGATATTCCAATA+AGG | 0.311508 | 6.3:-21219928 | MS.gene042579:CDS |
| ACATCAAGGTCATGAAATTC+AGG | 0.340788 | 6.3:+21219674 | None:intergenic |
| GTCGTAATTATGTGCCTACT+TGG | 0.343620 | 6.3:-21222504 | MS.gene042579:CDS |
| AAATCATGAATTCTTCTTTG+TGG | 0.356564 | 6.3:-21222606 | None:intergenic |
| ACCAAATAAATAAGACCCTT+TGG | 0.370193 | 6.3:+21221268 | None:intergenic |
| CTCAATGGCAAAGGCTAAGA+TGG | 0.402835 | 6.3:-21219649 | MS.gene042579:CDS |
| TTCAAGAACATCAATCAGTT+AGG | 0.408800 | 6.3:-21219902 | MS.gene042579:CDS |
| TGTGGATGATATTCCAATAA+GGG | 0.413494 | 6.3:-21219927 | MS.gene042579:CDS |
| ACTCCTGTTGATGTTCCATT+TGG | 0.413889 | 6.3:-21222526 | MS.gene042579:CDS |
| CCCTTTGTAAGAGGCTATGA+AGG | 0.414088 | 6.3:+21219772 | None:intergenic |
| AGAGGCTATGAAGGGTGCTT+TGG | 0.415798 | 6.3:+21219781 | None:intergenic |
| GTTTATGGAATGCTGATGAT+TGG | 0.416209 | 6.3:-21219838 | MS.gene042579:CDS |
| CGACCAAATGGAACATCAAC+AGG | 0.417482 | 6.3:+21222523 | None:intergenic |
| ATAAATAAGACCCTTTGGAT+TGG | 0.459969 | 6.3:+21221273 | None:intergenic |
| AATATGAAACCCTTTGTAAG+AGG | 0.460984 | 6.3:+21219763 | None:intergenic |
| ATCATAGATATTCTGTGTTG+TGG | 0.461108 | 6.3:-21220739 | MS.gene042579:CDS |
| TTGGGCCACAAGGGGTGGAT+TGG | 0.461492 | 6.3:-21219819 | MS.gene042579:CDS |
| ACATTTGTTTGTAAAATGTA+TGG | 0.464247 | 6.3:+21220827 | None:intergenic |
| GGAACATCAACAGGAGTCCT+TGG | 0.467558 | 6.3:+21222532 | None:intergenic |
| GGCACATAATTACGACCAAA+TGG | 0.477157 | 6.3:+21222511 | None:intergenic |
| GAGTTCTTAGGGAACAGAAC+AGG | 0.482915 | 6.3:-21220854 | MS.gene042579:CDS |
| TATGGAATGCAGTTTCTTTG+TGG | 0.485427 | 6.3:-21219945 | MS.gene042579:intron |
| TCAAGAACATCAATCAGTTA+GGG | 0.487977 | 6.3:-21219901 | MS.gene042579:CDS |
| TTACAAACAAATGTGTTCAC+AGG | 0.492984 | 6.3:-21220818 | MS.gene042579:CDS |
| TACACTCTTGAGGAACTTGA+GGG | 0.504511 | 6.3:+21219576 | None:intergenic |
| CCCTTCATAGCCTCTTACAA+AGG | 0.505336 | 6.3:-21219773 | MS.gene042579:CDS |
| CCTTCATAGCCTCTTACAAA+GGG | 0.513581 | 6.3:-21219772 | MS.gene042579:CDS |
| ATGGTACCTGGTGATTCAGC+TGG | 0.518135 | 6.3:-21221224 | MS.gene042579:CDS |
| TTTATGGAATGCTGATGATT+GGG | 0.520881 | 6.3:-21219837 | MS.gene042579:CDS |
| CTGTCTCTTGTACACTCTTG+AGG | 0.521195 | 6.3:+21219566 | None:intergenic |
| CTTAATCTTGATAAATCCAC+TGG | 0.526040 | 6.3:-21222436 | MS.gene042579:intron |
| ATGACCTTGATGTTGCTCAA+TGG | 0.530219 | 6.3:-21219664 | MS.gene042579:CDS |
| TCGTAATTATGTGCCTACTT+GGG | 0.537039 | 6.3:-21222503 | MS.gene042579:CDS |
| TTTGCCATTGAGCAACATCA+AGG | 0.537545 | 6.3:+21219660 | None:intergenic |
| GATGATTGGGCCACAAGGGG+TGG | 0.538500 | 6.3:-21219824 | MS.gene042579:CDS |
| CTTCAGTATGTACATAAGAA+TGG | 0.540587 | 6.3:-21221243 | MS.gene042579:CDS |
| TTTCTCCAATCCACCCCTTG+TGG | 0.544658 | 6.3:+21219814 | None:intergenic |
| GTACACTCTTGAGGAACTTG+AGG | 0.544701 | 6.3:+21219575 | None:intergenic |
| ATGCTGATGATTGGGCCACA+AGG | 0.558889 | 6.3:-21219829 | MS.gene042579:CDS |
| TGCTGATGATTGGGCCACAA+GGG | 0.560025 | 6.3:-21219828 | MS.gene042579:CDS |
| AATGTGTTCACAGGAGGCAA+AGG | 0.571333 | 6.3:-21220809 | MS.gene042579:CDS |
| TTGATGTTGCTCAATGGCAA+AGG | 0.585400 | 6.3:-21219658 | MS.gene042579:CDS |
| GTGGATTGGAGAAAACAGAT+TGG | 0.591719 | 6.3:-21219805 | MS.gene042579:CDS |
| GTACTGGTTTCCAATCCAAA+GGG | 0.594043 | 6.3:-21221283 | MS.gene042579:CDS |
| GGTACTGGTTTCCAATCCAA+AGG | 0.596981 | 6.3:-21221284 | MS.gene042579:CDS |
| CAAACAAATGTGTTCACAGG+AGG | 0.609686 | 6.3:-21220815 | MS.gene042579:CDS |
| CATATCAAATACTTAAATGG+TGG | 0.615790 | 6.3:-21222472 | MS.gene042579:CDS |
| TCAATGGCAAAGGCTAAGAT+GGG | 0.621555 | 6.3:-21219648 | MS.gene042579:CDS |
| GCTGATGATTGGGCCACAAG+GGG | 0.635602 | 6.3:-21219827 | MS.gene042579:CDS |
| ATATGATCATAAGCCCAAGT+AGG | 0.638602 | 6.3:+21222490 | None:intergenic |
| CCTTTGTAAGAGGCTATGAA+GGG | 0.655937 | 6.3:+21219773 | None:intergenic |
| ACTGTTCCAGCTGAATCACC+AGG | 0.670532 | 6.3:+21221218 | None:intergenic |
| ATGTACATAAGAATGGTACC+TGG | 0.672045 | 6.3:-21221236 | MS.gene042579:CDS |
| CAAGAACATCAATCAGTTAG+GGG | 0.677246 | 6.3:-21219900 | MS.gene042579:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGTTCTATAAAATAAAAA+AGG | - | chr6.3:21222033-21222052 | MS.gene042579:intron | 10.0% |
| !! | TTATTTCAAAATAAATTAGA+GGG | - | chr6.3:21220594-21220613 | MS.gene042579:intron | 10.0% |
| !! | TTTATTTCAAAATAAATTAG+AGG | - | chr6.3:21220593-21220612 | MS.gene042579:intron | 10.0% |
| !!! | AACAAAAATGATTTTTTTTT+AGG | + | chr6.3:21220824-21220843 | None:intergenic | 10.0% |
| !!! | ACTTATTTACATTTTTTTTT+TGG | - | chr6.3:21220041-21220060 | MS.gene042579:intron | 10.0% |
| !!! | AGTTTTATTATTATGATTTT+TGG | - | chr6.3:21220631-21220650 | MS.gene042579:intron | 10.0% |
| !!! | GATATAATTATTTTTTACTA+TGG | + | chr6.3:21221027-21221046 | None:intergenic | 10.0% |
| !!! | TAATTTATTTATTTTTTGAG+GGG | + | chr6.3:21221916-21221935 | None:intergenic | 10.0% |
| !!! | TTTACTTATTTACATTTTTT+TGG | - | chr6.3:21219863-21219882 | MS.gene042579:CDS | 10.0% |
| !! | AAAATTTACATTATCTAAGT+GGG | - | chr6.3:21220335-21220354 | MS.gene042579:intron | 15.0% |
| !! | AAATTATAGGATCAATATTA+TGG | + | chr6.3:21220207-21220226 | None:intergenic | 15.0% |
| !! | ATGTAAAGTCAAAATAAATT+AGG | + | chr6.3:21221231-21221250 | None:intergenic | 15.0% |
| !! | TAAAATTTACATTATCTAAG+TGG | - | chr6.3:21220334-21220353 | MS.gene042579:intron | 15.0% |
| !! | TGAAAATATACAATAGTTTA+TGG | - | chr6.3:21222304-21222323 | MS.gene042579:intron | 15.0% |
| !! | TTTATCGCAAATATAAAAAT+AGG | - | chr6.3:21220395-21220414 | MS.gene042579:intron | 15.0% |
| !!! | AAACTATTGTATATTTTCAT+TGG | + | chr6.3:21222303-21222322 | None:intergenic | 15.0% |
| !!! | AATTTATTTATTTTTTGAGG+GGG | + | chr6.3:21221915-21221934 | None:intergenic | 15.0% |
| !!! | AATTTATTTTGACTTTACAT+TGG | - | chr6.3:21221231-21221250 | MS.gene042579:CDS | 15.0% |
| !!! | ATAATAAAACTCTCTATTTT+TGG | + | chr6.3:21220622-21220641 | None:intergenic | 15.0% |
| !!! | ATTTTCTAAATTATCGTTTA+AGG | - | chr6.3:21222007-21222026 | MS.gene042579:intron | 15.0% |
| !!! | TAATAAAACTCTCTATTTTT+GGG | + | chr6.3:21220621-21220640 | None:intergenic | 15.0% |
| !!! | TTACAACAATATTTTTCTAT+AGG | - | chr6.3:21219782-21219801 | MS.gene042579:CDS | 15.0% |
| !!! | TTTGAAATAAAAAAATAGAG+AGG | + | chr6.3:21220584-21220603 | None:intergenic | 15.0% |
| !!! | TTTTCTAAATTATCGTTTAA+GGG | - | chr6.3:21222008-21222027 | MS.gene042579:intron | 15.0% |
| !! | AAAAAAAAATCACATAGTGT+TGG | + | chr6.3:21220020-21220039 | None:intergenic | 20.0% |
| !! | AAAAACCTTATTATCTTTCT+TGG | + | chr6.3:21221646-21221665 | None:intergenic | 20.0% |
| !! | ACAACAAAAATCATGTTAAA+AGG | + | chr6.3:21222137-21222156 | None:intergenic | 20.0% |
| !! | ACATTCATATTCTTAAAACT+GGG | + | chr6.3:21220682-21220701 | None:intergenic | 20.0% |
| !! | ACATTTGTTTGTAAAATGTA+TGG | + | chr6.3:21221333-21221352 | None:intergenic | 20.0% |
| !! | ATTTGGATTTAAAAAAACGT+AGG | + | chr6.3:21221854-21221873 | None:intergenic | 20.0% |
| !! | CTCTTATAAAAACTCAAATA+TGG | - | chr6.3:21222194-21222213 | MS.gene042579:intron | 20.0% |
| !! | GATCATATCAAATACTTAAA+TGG | - | chr6.3:21219682-21219701 | MS.gene042579:CDS | 20.0% |
| !! | TAATAAGTACTTACACAATT+TGG | + | chr6.3:21221452-21221471 | None:intergenic | 20.0% |
| !! | TACATTCATATTCTTAAAAC+TGG | + | chr6.3:21220683-21220702 | None:intergenic | 20.0% |
| !!! | AAACTTTAGAGTTCAATTTT+GGG | - | chr6.3:21221550-21221569 | MS.gene042579:intron | 20.0% |
| !!! | ATAAGGAATGTACTTTTATA+GGG | - | chr6.3:21220509-21220528 | MS.gene042579:intron | 20.0% |
| !!! | ATATTTTAATATCCCTACTT+AGG | - | chr6.3:21220085-21220104 | MS.gene042579:intron | 20.0% |
| !!! | GTTTGATTTTTATATACGAT+CGG | + | chr6.3:21221163-21221182 | None:intergenic | 20.0% |
| !!! | TAAAGTTTGAGGTAAAAATA+TGG | + | chr6.3:21221538-21221557 | None:intergenic | 20.0% |
| !!! | TATAAGGAATGTACTTTTAT+AGG | - | chr6.3:21220508-21220527 | MS.gene042579:intron | 20.0% |
| !!! | TATTGTATATTTTCATTGGT+TGG | + | chr6.3:21222299-21222318 | None:intergenic | 20.0% |
| !!! | TCAGACATTTTGAAAATTAT+AGG | + | chr6.3:21220220-21220239 | None:intergenic | 20.0% |
| ! | AAAAAAAGTCACATAGTGTT+CGG | + | chr6.3:21219842-21219861 | None:intergenic | 25.0% |
| ! | AACTACCAAGAAAGATAATA+AGG | - | chr6.3:21221638-21221657 | MS.gene042579:intron | 25.0% |
| ! | ACTAATAGACAATTAGGAAA+AGG | + | chr6.3:21220797-21220816 | None:intergenic | 25.0% |
| ! | CATATCAAATACTTAAATGG+TGG | - | chr6.3:21219685-21219704 | MS.gene042579:CDS | 25.0% |
| ! | CATTCATATTCTTAAAACTG+GGG | + | chr6.3:21220681-21220700 | None:intergenic | 25.0% |
| ! | GGTATAACTAATAGACAATT+AGG | + | chr6.3:21220803-21220822 | None:intergenic | 25.0% |
| ! | GTAAATTTGTAACCTAAGTA+GGG | + | chr6.3:21220100-21220119 | None:intergenic | 25.0% |
| ! | TGTAAATTTGTAACCTAAGT+AGG | + | chr6.3:21220101-21220120 | None:intergenic | 25.0% |
| ! | TTATGATCATTAGAGTCAAA+TGG | + | chr6.3:21221601-21221620 | None:intergenic | 25.0% |
| ! | TTCTTACATCAGCATTTATA+AGG | - | chr6.3:21220492-21220511 | MS.gene042579:intron | 25.0% |
| !! | ATGCAATTTTTGTACATAAC+AGG | - | chr6.3:21220852-21220871 | MS.gene042579:CDS | 25.0% |
| !! | TATCTATGATAAGCTTTTGT+AGG | + | chr6.3:21221408-21221427 | None:intergenic | 25.0% |
| !!! | AAATTGAACTCTAAAGTTTG+AGG | + | chr6.3:21221549-21221568 | None:intergenic | 25.0% |
| !!! | AGATAGATTTTGAGTTCTTA+GGG | - | chr6.3:21221292-21221311 | MS.gene042579:intron | 25.0% |
| !!! | ATTTTCATTGGTTGGTTAAA+TGG | + | chr6.3:21222291-21222310 | None:intergenic | 25.0% |
| !!! | CAAACTTTAGAGTTCAATTT+TGG | - | chr6.3:21221549-21221568 | MS.gene042579:intron | 25.0% |
| !!! | CTTTTATAGGGTGTTAAATA+TGG | - | chr6.3:21220521-21220540 | MS.gene042579:intron | 25.0% |
| !!! | TTGATGATGTGATATTGTTT+AGG | - | chr6.3:21220280-21220299 | MS.gene042579:intron | 25.0% |
| !!! | TTTTCATTGGTTGGTTAAAT+GGG | + | chr6.3:21222290-21222309 | None:intergenic | 25.0% |
| !!! | TTTTTTTGAAGAGTAGTCTA+CGG | + | chr6.3:21220710-21220729 | None:intergenic | 25.0% |
| AAATGATAATAGCATACCAG+TGG | + | chr6.3:21219740-21219759 | None:intergenic | 30.0% | |
| AATATGAAACCCTTTGTAAG+AGG | + | chr6.3:21222397-21222416 | None:intergenic | 30.0% | |
| AATATGGATGATGAATTGTG+TGG | + | chr6.3:21221522-21221541 | None:intergenic | 30.0% | |
| ACATCTTAATGCTTCTACTT+AGG | - | chr6.3:21219907-21219926 | MS.gene042579:CDS | 30.0% | |
| ACCAAATAAATAAGACCCTT+TGG | + | chr6.3:21220892-21220911 | None:intergenic | 30.0% | |
| ATAAATAAGACCCTTTGGAT+TGG | + | chr6.3:21220887-21220906 | None:intergenic | 30.0% | |
| ATATGGATGATGAATTGTGT+GGG | + | chr6.3:21221521-21221540 | None:intergenic | 30.0% | |
| ATCATAGATATTCTGTGTTG+TGG | - | chr6.3:21221418-21221437 | MS.gene042579:intron | 30.0% | |
| CAAATTTACATTGCCAAACT+CGG | - | chr6.3:21220111-21220130 | MS.gene042579:intron | 30.0% | |
| CTTAATCTTGATAAATCCAC+TGG | - | chr6.3:21219721-21219740 | MS.gene042579:CDS | 30.0% | |
| CTTCAGTATGTACATAAGAA+TGG | - | chr6.3:21220914-21220933 | MS.gene042579:intron | 30.0% | |
| GAGAACAAAGAATCTATCTT+TGG | - | chr6.3:21221376-21221395 | MS.gene042579:intron | 30.0% | |
| TACAAAGGGTTTCATATTGA+TGG | - | chr6.3:21222399-21222418 | MS.gene042579:intron | 30.0% | |
| TCAAGAACATCAATCAGTTA+GGG | - | chr6.3:21222256-21222275 | MS.gene042579:intron | 30.0% | |
| TGTAAATAATAAGCCGAGTT+TGG | + | chr6.3:21220127-21220146 | None:intergenic | 30.0% | |
| TGTGGATGATATTCCAATAA+GGG | - | chr6.3:21222230-21222249 | MS.gene042579:intron | 30.0% | |
| TTACAAACAAATGTGTTCAC+AGG | - | chr6.3:21221339-21221358 | MS.gene042579:intron | 30.0% | |
| TTCAAGAACATCAATCAGTT+AGG | - | chr6.3:21222255-21222274 | MS.gene042579:intron | 30.0% | |
| TTCTATAGGTTTCTATGTTG+AGG | - | chr6.3:21219796-21219815 | MS.gene042579:CDS | 30.0% | |
| TTGTGGATGATATTCCAATA+AGG | - | chr6.3:21222229-21222248 | MS.gene042579:intron | 30.0% | |
| TTTATGGAATGCTGATGATT+GGG | - | chr6.3:21222320-21222339 | MS.gene042579:intron | 30.0% | |
| ! | TTTTTGTACATAACAGGTAC+TGG | - | chr6.3:21220858-21220877 | MS.gene042579:CDS | 30.0% |
| !! | AAGTAGAAGCATTAAGATGT+TGG | + | chr6.3:21219907-21219926 | None:intergenic | 30.0% |
| !! | ATTTTGAGAAGATAACTGCA+TGG | + | chr6.3:21221259-21221278 | None:intergenic | 30.0% |
| !! | TCCAAAGGGTCTTATTTATT+TGG | - | chr6.3:21220888-21220907 | MS.gene042579:CDS | 30.0% |
| !!! | GAGATAGATTTTGAGTTCTT+AGG | - | chr6.3:21221291-21221310 | MS.gene042579:intron | 30.0% |
| AAAATGTCTGATGACCTATG+TGG | - | chr6.3:21220229-21220248 | MS.gene042579:intron | 35.0% | |
| AAGCTTATCACTTACCACAT+AGG | + | chr6.3:21220246-21220265 | None:intergenic | 35.0% | |
| ACATCAAGGTCATGAAATTC+AGG | + | chr6.3:21222486-21222505 | None:intergenic | 35.0% | |
| AGAGTTAACGCATCAACATT+TGG | + | chr6.3:21221871-21221890 | None:intergenic | 35.0% | |
| ATATGATCATAAGCCCAAGT+AGG | + | chr6.3:21219670-21219689 | None:intergenic | 35.0% | |
| ATGTACATAAGAATGGTACC+TGG | - | chr6.3:21220921-21220940 | MS.gene042579:intron | 35.0% | |
| ATGTTCTTGAACACCCTTAT+TGG | + | chr6.3:21222246-21222265 | None:intergenic | 35.0% | |
| CAAGAACATCAATCAGTTAG+GGG | - | chr6.3:21222257-21222276 | MS.gene042579:intron | 35.0% | |
| GTTTATGGAATGCTGATGAT+TGG | - | chr6.3:21222319-21222338 | MS.gene042579:intron | 35.0% | |
| TATGGAATGCAGTTTCTTTG+TGG | - | chr6.3:21222212-21222231 | MS.gene042579:intron | 35.0% | |
| TCATTAGAGTCAAATGGAGA+AGG | + | chr6.3:21221595-21221614 | None:intergenic | 35.0% | |
| TCGTAATTATGTGCCTACTT+GGG | - | chr6.3:21219654-21219673 | MS.gene042579:CDS | 35.0% | |
| TGGTAAGTGATAAGCTTATG+TGG | - | chr6.3:21220249-21220268 | MS.gene042579:intron | 35.0% | |
| ! | AAAAAACGTAGGCTTTTGAG+AGG | + | chr6.3:21221843-21221862 | None:intergenic | 35.0% |
| ! | GCAAAATTTTGTGACACACA+AGG | - | chr6.3:21222441-21222460 | MS.gene042579:CDS | 35.0% |
| !! | AAAAACGTAGGCTTTTGAGA+GGG | + | chr6.3:21221842-21221861 | None:intergenic | 35.0% |
| ACACACAAGGAAAAAGATGG+TGG | - | chr6.3:21222454-21222473 | MS.gene042579:CDS | 40.0% | |
| ACTCCTGTTGATGTTCCATT+TGG | - | chr6.3:21219631-21219650 | MS.gene042579:CDS | 40.0% | |
| ATGACCTTGATGTTGCTCAA+TGG | - | chr6.3:21222493-21222512 | MS.gene042579:CDS | 40.0% | |
| CAAACAAATGTGTTCACAGG+AGG | - | chr6.3:21221342-21221361 | MS.gene042579:intron | 40.0% | |
| CACACAAGGAAAAAGATGGT+GGG | - | chr6.3:21222455-21222474 | MS.gene042579:CDS | 40.0% | |
| CCTTCATAGCCTCTTACAAA+GGG | - | chr6.3:21222385-21222404 | MS.gene042579:intron | 40.0% | |
| CCTTTGTAAGAGGCTATGAA+GGG | + | chr6.3:21222387-21222406 | None:intergenic | 40.0% | |
| GGCACATAATTACGACCAAA+TGG | + | chr6.3:21219649-21219668 | None:intergenic | 40.0% | |
| GTCGTAATTATGTGCCTACT+TGG | - | chr6.3:21219653-21219672 | MS.gene042579:CDS | 40.0% | |
| GTGACACACAAGGAAAAAGA+TGG | - | chr6.3:21222451-21222470 | MS.gene042579:CDS | 40.0% | |
| TACACTCTTGAGGAACTTGA+GGG | + | chr6.3:21222584-21222603 | None:intergenic | 40.0% | |
| TCAATGGCAAAGGCTAAGAT+GGG | - | chr6.3:21222509-21222528 | MS.gene042579:CDS | 40.0% | |
| TTGATGTTGCTCAATGGCAA+AGG | - | chr6.3:21222499-21222518 | MS.gene042579:CDS | 40.0% | |
| TTTGCCATTGAGCAACATCA+AGG | + | chr6.3:21222500-21222519 | None:intergenic | 40.0% | |
| ! | CTTCTTTTGCTGCAAATCCA+AGG | - | chr6.3:21219608-21219627 | MS.gene042579:CDS | 40.0% |
| !! | GTACTGGTTTCCAATCCAAA+GGG | - | chr6.3:21220874-21220893 | MS.gene042579:CDS | 40.0% |
| !! | GTGGATTGGAGAAAACAGAT+TGG | - | chr6.3:21222352-21222371 | MS.gene042579:intron | 40.0% |
| AATGTGTTCACAGGAGGCAA+AGG | - | chr6.3:21221348-21221367 | MS.gene042579:intron | 45.0% | |
| CCCTTCATAGCCTCTTACAA+AGG | - | chr6.3:21222384-21222403 | MS.gene042579:intron | 45.0% | |
| CCCTTTGTAAGAGGCTATGA+AGG | + | chr6.3:21222388-21222407 | None:intergenic | 45.0% | |
| CGACCAAATGGAACATCAAC+AGG | + | chr6.3:21219637-21219656 | None:intergenic | 45.0% | |
| CTCAATGGCAAAGGCTAAGA+TGG | - | chr6.3:21222508-21222527 | MS.gene042579:CDS | 45.0% | |
| CTGTCTCTTGTACACTCTTG+AGG | + | chr6.3:21222594-21222613 | None:intergenic | 45.0% | |
| GAGTTCTTAGGGAACAGAAC+AGG | - | chr6.3:21221303-21221322 | MS.gene042579:intron | 45.0% | |
| GTACACTCTTGAGGAACTTG+AGG | + | chr6.3:21222585-21222604 | None:intergenic | 45.0% | |
| TTCGGTAGCGCCTCAAATAA+GGG | + | chr6.3:21219824-21219843 | None:intergenic | 45.0% | |
| ! | TGTTGAGGTGCCCTTATTTG+AGG | - | chr6.3:21219811-21219830 | MS.gene042579:CDS | 45.0% |
| !! | GGTACTGGTTTCCAATCCAA+AGG | - | chr6.3:21220873-21220892 | MS.gene042579:CDS | 45.0% |
| !!! | ATTAATTTATTTATTTTTTG+AGG | + | chr6.3:21221918-21221937 | None:intergenic | 5.0% |
| !!! | TTAATTTATTTATTTTTTGA+GGG | + | chr6.3:21221917-21221936 | None:intergenic | 5.0% |
| ACTGTTCCAGCTGAATCACC+AGG | + | chr6.3:21220942-21220961 | None:intergenic | 50.0% | |
| ATGCTGATGATTGGGCCACA+AGG | - | chr6.3:21222328-21222347 | MS.gene042579:intron | 50.0% | |
| ATGGTACCTGGTGATTCAGC+TGG | - | chr6.3:21220933-21220952 | MS.gene042579:intron | 50.0% | |
| GGAACATCAACAGGAGTCCT+TGG | + | chr6.3:21219628-21219647 | None:intergenic | 50.0% | |
| GTTCGGTAGCGCCTCAAATA+AGG | + | chr6.3:21219825-21219844 | None:intergenic | 50.0% | |
| TGCTGATGATTGGGCCACAA+GGG | - | chr6.3:21222329-21222348 | MS.gene042579:intron | 50.0% | |
| TTTCTCCAATCCACCCCTTG+TGG | + | chr6.3:21222346-21222365 | None:intergenic | 50.0% | |
| !! | AGAGGCTATGAAGGGTGCTT+TGG | + | chr6.3:21222379-21222398 | None:intergenic | 50.0% |
| GCTGATGATTGGGCCACAAG+GGG | - | chr6.3:21222330-21222349 | MS.gene042579:intron | 55.0% | |
| GATGATTGGGCCACAAGGGG+TGG | - | chr6.3:21222333-21222352 | MS.gene042579:intron | 60.0% | |
| TTGGGCCACAAGGGGTGGAT+TGG | - | chr6.3:21222338-21222357 | MS.gene042579:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 21219556 | 21222623 | 21219556 | ID=MS.gene042579 |
| chr6.3 | mRNA | 21219556 | 21222623 | 21219556 | ID=MS.gene042579.t1;Parent=MS.gene042579 |
| chr6.3 | exon | 21222437 | 21222623 | 21222437 | ID=MS.gene042579.t1.exon1;Parent=MS.gene042579.t1 |
| chr6.3 | CDS | 21222437 | 21222623 | 21222437 | ID=cds.MS.gene042579.t1;Parent=MS.gene042579.t1 |
| chr6.3 | exon | 21221205 | 21221305 | 21221205 | ID=MS.gene042579.t1.exon2;Parent=MS.gene042579.t1 |
| chr6.3 | CDS | 21221205 | 21221305 | 21221205 | ID=cds.MS.gene042579.t1;Parent=MS.gene042579.t1 |
| chr6.3 | exon | 21220722 | 21220915 | 21220722 | ID=MS.gene042579.t1.exon3;Parent=MS.gene042579.t1 |
| chr6.3 | CDS | 21220722 | 21220915 | 21220722 | ID=cds.MS.gene042579.t1;Parent=MS.gene042579.t1 |
| chr6.3 | exon | 21219556 | 21219955 | 21219556 | ID=MS.gene042579.t1.exon4;Parent=MS.gene042579.t1 |
| chr6.3 | CDS | 21219556 | 21219955 | 21219556 | ID=cds.MS.gene042579.t1;Parent=MS.gene042579.t1 |
| Gene Sequence |
| Protein sequence |
