Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04393.t1 | XP_003600461.1 | 100 | 313 | 0 | 0 | 1 | 313 | 1 | 313 | 2.10E-186 | 661.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04393.t1 | Q9FKT5 | 85.6 | 313 | 44 | 1 | 2 | 313 | 3 | 315 | 6.9e-162 | 571.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04393.t1 | G7IY98 | 100.0 | 313 | 0 | 0 | 1 | 313 | 1 | 313 | 1.5e-186 | 661.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene04393 | MS.gene71431 | PPI |
| MS.gene95244 | MS.gene04393 | PPI |
| MS.gene97981 | MS.gene04393 | PPI |
| MS.gene68222 | MS.gene04393 | PPI |
| MS.gene064894 | MS.gene04393 | PPI |
| MS.gene33535 | MS.gene04393 | PPI |
| MS.gene04393 | MS.gene063165 | PPI |
| MS.gene056487 | MS.gene04393 | PPI |
| MS.gene04393 | MS.gene008794 | PPI |
| MS.gene066026 | MS.gene04393 | PPI |
| MS.gene008793 | MS.gene04393 | PPI |
| MS.gene04393 | MS.gene014281 | PPI |
| MS.gene52980 | MS.gene04393 | PPI |
| MS.gene04393 | MS.gene93058 | PPI |
| MS.gene042253 | MS.gene04393 | PPI |
| MS.gene004825 | MS.gene04393 | PPI |
| MS.gene04393 | MS.gene012608 | PPI |
| MS.gene046235 | MS.gene04393 | PPI |
| MS.gene056488 | MS.gene04393 | PPI |
| MS.gene04393 | MS.gene97980 | PPI |
| MS.gene93059 | MS.gene04393 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04393.t1 | MTR_3g061450 | 100.000 | 313 | 0 | 0 | 1 | 313 | 1 | 313 | 0.0 | 658 |
| MS.gene04393.t1 | MTR_4g095058 | 28.179 | 291 | 190 | 8 | 11 | 289 | 280 | 563 | 4.33e-21 | 94.0 |
| MS.gene04393.t1 | MTR_4g095058 | 28.962 | 183 | 109 | 5 | 8 | 177 | 402 | 576 | 9.75e-15 | 75.1 |
| MS.gene04393.t1 | MTR_4g095058 | 28.179 | 291 | 190 | 8 | 11 | 289 | 278 | 561 | 4.48e-21 | 94.0 |
| MS.gene04393.t1 | MTR_4g095058 | 28.962 | 183 | 109 | 5 | 8 | 177 | 400 | 574 | 9.73e-15 | 75.1 |
| MS.gene04393.t1 | MTR_5g016350 | 24.625 | 333 | 204 | 9 | 17 | 307 | 167 | 494 | 8.90e-17 | 80.9 |
| MS.gene04393.t1 | MTR_5g016350 | 29.775 | 178 | 104 | 5 | 13 | 177 | 348 | 517 | 3.33e-15 | 76.3 |
| MS.gene04393.t1 | MTR_5g016350 | 25.177 | 282 | 152 | 10 | 20 | 249 | 231 | 505 | 4.57e-13 | 69.7 |
| MS.gene04393.t1 | MTR_5g016350 | 24.625 | 333 | 204 | 9 | 17 | 307 | 231 | 558 | 9.40e-17 | 81.3 |
| MS.gene04393.t1 | MTR_5g016350 | 29.775 | 178 | 104 | 5 | 13 | 177 | 412 | 581 | 2.08e-15 | 77.0 |
| MS.gene04393.t1 | MTR_5g016350 | 25.177 | 282 | 152 | 10 | 20 | 249 | 295 | 569 | 4.55e-13 | 70.1 |
| MS.gene04393.t1 | MTR_5g016370 | 24.625 | 333 | 202 | 11 | 17 | 307 | 245 | 570 | 4.85e-16 | 79.0 |
| MS.gene04393.t1 | MTR_5g016370 | 28.409 | 176 | 109 | 4 | 13 | 177 | 424 | 593 | 6.64e-16 | 78.6 |
| MS.gene04393.t1 | MTR_5g016370 | 23.759 | 282 | 156 | 7 | 20 | 249 | 307 | 581 | 1.09e-11 | 65.9 |
| MS.gene04393.t1 | MTR_4g125710 | 24.254 | 268 | 177 | 6 | 13 | 258 | 54 | 317 | 9.59e-16 | 77.0 |
| MS.gene04393.t1 | MTR_3g106080 | 25.882 | 255 | 124 | 6 | 15 | 251 | 457 | 664 | 1.30e-15 | 77.8 |
| MS.gene04393.t1 | MTR_1g097140 | 26.154 | 260 | 180 | 7 | 12 | 267 | 116 | 367 | 2.71e-15 | 76.3 |
| MS.gene04393.t1 | MTR_1g072140 | 23.051 | 295 | 213 | 8 | 11 | 297 | 19 | 307 | 3.22e-15 | 75.5 |
| MS.gene04393.t1 | MTR_4g119620 | 24.797 | 246 | 172 | 6 | 13 | 251 | 72 | 311 | 1.47e-14 | 73.6 |
| MS.gene04393.t1 | MTR_3g449990 | 25.000 | 288 | 202 | 9 | 17 | 295 | 268 | 550 | 5.56e-14 | 72.8 |
| MS.gene04393.t1 | MTR_7g086360 | 21.141 | 298 | 218 | 7 | 16 | 301 | 52 | 344 | 1.61e-13 | 70.5 |
| MS.gene04393.t1 | MTR_5g090420 | 24.739 | 287 | 201 | 9 | 18 | 295 | 263 | 543 | 2.33e-13 | 70.9 |
| MS.gene04393.t1 | MTR_6g004040 | 27.160 | 243 | 158 | 8 | 13 | 244 | 5 | 239 | 1.41e-12 | 68.6 |
| MS.gene04393.t1 | MTR_5g022690 | 25.238 | 210 | 145 | 6 | 13 | 217 | 273 | 475 | 4.18e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04393.t1 | AT5G56130 | 85.714 | 315 | 43 | 2 | 1 | 313 | 1 | 315 | 0.0 | 570 |
| MS.gene04393.t1 | AT5G67320 | 28.669 | 293 | 186 | 11 | 11 | 289 | 315 | 598 | 7.55e-22 | 96.3 |
| MS.gene04393.t1 | AT5G67320 | 29.714 | 175 | 110 | 4 | 12 | 177 | 441 | 611 | 6.47e-15 | 75.5 |
| MS.gene04393.t1 | AT2G43770 | 23.729 | 295 | 208 | 8 | 16 | 298 | 50 | 339 | 1.08e-16 | 80.1 |
| MS.gene04393.t1 | AT5G08560 | 25.260 | 289 | 196 | 9 | 31 | 311 | 286 | 562 | 1.05e-14 | 75.1 |
| MS.gene04393.t1 | AT5G08560 | 25.260 | 289 | 196 | 9 | 31 | 311 | 286 | 562 | 1.05e-14 | 75.1 |
| MS.gene04393.t1 | AT5G08560 | 25.260 | 289 | 196 | 9 | 31 | 311 | 286 | 562 | 1.05e-14 | 75.1 |
| MS.gene04393.t1 | AT5G25150 | 25.292 | 257 | 123 | 7 | 15 | 251 | 456 | 663 | 1.17e-14 | 75.1 |
| MS.gene04393.t1 | AT4G02730 | 27.358 | 212 | 140 | 8 | 14 | 217 | 80 | 285 | 9.88e-14 | 71.2 |
| MS.gene04393.t1 | AT3G49660 | 25.000 | 304 | 199 | 12 | 10 | 295 | 15 | 307 | 1.05e-13 | 70.9 |
| MS.gene04393.t1 | AT3G49660 | 23.715 | 253 | 180 | 6 | 13 | 258 | 65 | 311 | 1.78e-13 | 70.5 |
| MS.gene04393.t1 | AT3G49660 | 26.442 | 208 | 140 | 6 | 17 | 217 | 111 | 312 | 5.07e-11 | 63.2 |
| MS.gene04393.t1 | AT5G25150 | 27.143 | 210 | 100 | 6 | 15 | 220 | 456 | 616 | 1.80e-13 | 71.2 |
Find 74 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGATTCCCTTGTCAGCTTA+TGG | 0.152266 | 3.4:-57283879 | MS.gene04393:CDS |
| TATGCAAGTACATTGTATTT+AGG | 0.212077 | 3.4:+57283121 | None:intergenic |
| TTGATCAAACTGCTCGAATT+TGG | 0.221602 | 3.4:-57290209 | MS.gene04393:CDS |
| ACAATTAGTTTCAATCATAC+TGG | 0.283022 | 3.4:-57283691 | MS.gene04393:CDS |
| TGCAATAAGATCGGGATGTT+TGG | 0.289165 | 3.4:+57289191 | None:intergenic |
| TGATTCCCTTGTCAGCTTAT+GGG | 0.291294 | 3.4:-57283878 | MS.gene04393:CDS |
| GCAATAAGATCGGGATGTTT+GGG | 0.297004 | 3.4:+57289192 | None:intergenic |
| GTGACAAGACTGTTCGTCTA+TGG | 0.297252 | 3.4:-57289158 | MS.gene04393:CDS |
| CGATCTTATTGCAACTGCTT+CGG | 0.300112 | 3.4:-57289181 | MS.gene04393:CDS |
| CTCATGTAGCTGTAGGTAAT+AGG | 0.308212 | 3.4:-57288876 | MS.gene04393:CDS |
| GATCTTATTGCAACTGCTTC+GGG | 0.329060 | 3.4:-57289180 | MS.gene04393:CDS |
| TGTCAGCTTATGGGTTATTT+CGG | 0.355336 | 3.4:-57283869 | MS.gene04393:CDS |
| TTCTTTCTAACAACAGGAAA+TGG | 0.391405 | 3.4:-57284295 | MS.gene04393:intron |
| CAGTACATCACATTCCTTGT+AGG | 0.411919 | 3.4:-57283171 | MS.gene04393:CDS |
| CTTCACAGCAGAGAGTATTC+TGG | 0.419755 | 3.4:-57290450 | MS.gene04393:CDS |
| TGCATTGCAATTGACCCAAC+AGG | 0.430132 | 3.4:-57284076 | MS.gene04393:CDS |
| AAAAGGTGCATTCAGTGGCT+TGG | 0.441542 | 3.4:-57290266 | MS.gene04393:intron |
| CCTAGGTAAATGAGATTGCT+TGG | 0.445389 | 3.4:-57284336 | MS.gene04393:intron |
| ATGAGTCCCATCAGGTTTGT+AGG | 0.446382 | 3.4:+57288894 | None:intergenic |
| GGTCAAAGATATTGAATTGA+AGG | 0.446383 | 3.4:-57289250 | MS.gene04393:CDS |
| AGTTAACAATACTTGATGTT+CGG | 0.447811 | 3.4:-57288630 | MS.gene04393:CDS |
| TCATGTAGCTGTAGGTAATA+GGG | 0.452448 | 3.4:-57288875 | MS.gene04393:intron |
| GAGATGTTCTTTCTAACAAC+AGG | 0.457171 | 3.4:-57284301 | MS.gene04393:CDS |
| ATTGGCACAAAACTTGCTTC+TGG | 0.457664 | 3.4:-57290237 | MS.gene04393:CDS |
| CAATTAGTTTCAATCATACT+GGG | 0.469646 | 3.4:-57283690 | MS.gene04393:CDS |
| TATGATTGAAACTAATTGTC+CGG | 0.470565 | 3.4:+57283695 | None:intergenic |
| TACAATGTACTTGCATATGC+TGG | 0.472545 | 3.4:-57283115 | MS.gene04393:CDS |
| TGTTTCTCAGATGGCCTGTC+CGG | 0.480041 | 3.4:-57283714 | MS.gene04393:intron |
| AGGTGGAAAATGCTCACAAC+AGG | 0.493748 | 3.4:-57288947 | MS.gene04393:CDS |
| CGAAGCAGTTGCAATAAGAT+CGG | 0.502914 | 3.4:+57289182 | None:intergenic |
| CACAACAGGCAGAACTTAGT+GGG | 0.503581 | 3.4:-57288933 | MS.gene04393:CDS |
| CTATTCATTGCAGCCCTACA+AGG | 0.504824 | 3.4:+57283157 | None:intergenic |
| GTATCAAGAGGTCGAAGAGA+TGG | 0.505442 | 3.4:+57284127 | None:intergenic |
| TTGAACTTGCGCCTATGCAT+TGG | 0.510346 | 3.4:+57288598 | None:intergenic |
| TAGTGTGGATCAGCTATGTT+GGG | 0.512894 | 3.4:-57289217 | MS.gene04393:CDS |
| TGACAAGACTGTTCGTCTAT+GGG | 0.513043 | 3.4:-57289157 | MS.gene04393:CDS |
| AACTTACTCGAGCTTTGTAA+AGG | 0.513452 | 3.4:+57283828 | None:intergenic |
| AAGAAGCATACCTTCCTGTT+GGG | 0.521567 | 3.4:+57284062 | None:intergenic |
| TCAGTGGCTTGGAATTGCAT+TGG | 0.521845 | 3.4:-57290255 | MS.gene04393:CDS |
| AAATGAGAATTGTTCCTCCA+TGG | 0.522481 | 3.4:+57290479 | None:intergenic |
| TCACAACAGGCAGAACTTAG+TGG | 0.530916 | 3.4:-57288934 | MS.gene04393:CDS |
| GAAGCAGTTGCAATAAGATC+GGG | 0.535149 | 3.4:+57289183 | None:intergenic |
| AACATCACCTACAAACCTGA+TGG | 0.546702 | 3.4:-57288901 | MS.gene04393:CDS |
| CAAGAAGCATACCTTCCTGT+TGG | 0.550714 | 3.4:+57284061 | None:intergenic |
| TACTCTCATGGCTCATACAG+CGG | 0.555050 | 3.4:-57284107 | MS.gene04393:CDS |
| ACTCTCATGGCTCATACAGC+GGG | 0.555763 | 3.4:-57284106 | MS.gene04393:CDS |
| ATAGTGTGGATCAGCTATGT+TGG | 0.561550 | 3.4:-57289218 | MS.gene04393:CDS |
| TCGACCTCTTGATACTCTCA+TGG | 0.565472 | 3.4:-57284119 | MS.gene04393:CDS |
| ACAGCTACATGAGTCCCATC+AGG | 0.567301 | 3.4:+57288886 | None:intergenic |
| AGTACATCACATTCCTTGTA+GGG | 0.574588 | 3.4:-57283170 | MS.gene04393:CDS |
| GTCAAAGATATTGAATTGAA+GGG | 0.579788 | 3.4:-57289249 | MS.gene04393:CDS |
| GCTCACCTGCTCAGGGACTG+TGG | 0.582588 | 3.4:-57284164 | MS.gene04393:intron |
| GGAAATTCAAACCAATGCAT+AGG | 0.586683 | 3.4:-57288609 | MS.gene04393:CDS |
| TTGAAACTAATTGTCCGGAC+AGG | 0.589710 | 3.4:+57283700 | None:intergenic |
| AAGGTTACTACATGGGCATG+TGG | 0.595342 | 3.4:+57290177 | None:intergenic |
| TAGGCGCAAGTTCAACTATG+AGG | 0.599831 | 3.4:-57288590 | MS.gene04393:intron |
| TTTCGGAGATGCTTTGTGTG+CGG | 0.599990 | 3.4:-57283852 | MS.gene04393:CDS |
| GAGATTGCTTGGAACATGAC+TGG | 0.603670 | 3.4:-57284325 | MS.gene04393:CDS |
| GAAGGGTCACACCGATAGTG+TGG | 0.604201 | 3.4:-57289232 | MS.gene04393:CDS |
| GATGGGACTCATGTAGCTGT+AGG | 0.614418 | 3.4:-57288883 | MS.gene04393:CDS |
| AAATAACCCATAAGCTGACA+AGG | 0.614518 | 3.4:+57283872 | None:intergenic |
| AGGTTACTACATGGGCATGT+GGG | 0.614543 | 3.4:+57290178 | None:intergenic |
| CAATAAGATCGGGATGTTTG+GGG | 0.614895 | 3.4:+57289193 | None:intergenic |
| ACATAGCTGATCCACACTAT+CGG | 0.628331 | 3.4:+57289221 | None:intergenic |
| AACAAGTATCAGGCAGACGA+AGG | 0.633197 | 3.4:-57283082 | MS.gene04393:intron |
| ACATCACCTACAAACCTGAT+GGG | 0.634892 | 3.4:-57288900 | MS.gene04393:CDS |
| GTTTGAAAAGGTGCATTCAG+TGG | 0.648774 | 3.4:-57290271 | MS.gene04393:intron |
| CTGCAATGAATAGTGTTGAG+TGG | 0.656405 | 3.4:-57283147 | MS.gene04393:CDS |
| TTGCAATTGACCCAACAGGA+AGG | 0.660962 | 3.4:-57284072 | MS.gene04393:intron |
| TAACAGTCCAATGTTCACAC+CGG | 0.676215 | 3.4:-57283199 | MS.gene04393:intron |
| AATAACCCATAAGCTGACAA+GGG | 0.694844 | 3.4:+57283873 | None:intergenic |
| AATTAGTTTCAATCATACTG+GGG | 0.695286 | 3.4:-57283689 | MS.gene04393:CDS |
| TGAGCCATGAGAGTATCAAG+AGG | 0.696186 | 3.4:+57284115 | None:intergenic |
| ACAACAGGCAGAACTTAGTG+GGG | 0.714393 | 3.4:-57288932 | MS.gene04393:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 57282961 | 57290499 | 57282961 | ID=MS.gene04393 |
| chr3.4 | mRNA | 57282961 | 57290499 | 57282961 | ID=MS.gene04393.t1;Parent=MS.gene04393 |
| chr3.4 | exon | 57290437 | 57290499 | 57290437 | ID=MS.gene04393.t1.exon1;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57290437 | 57290499 | 57290437 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| chr3.4 | exon | 57290188 | 57290283 | 57290188 | ID=MS.gene04393.t1.exon2;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57290188 | 57290283 | 57290188 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| chr3.4 | exon | 57289148 | 57289277 | 57289148 | ID=MS.gene04393.t1.exon3;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57289148 | 57289277 | 57289148 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| chr3.4 | exon | 57288876 | 57288967 | 57288876 | ID=MS.gene04393.t1.exon4;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57288876 | 57288967 | 57288876 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| chr3.4 | exon | 57288591 | 57288659 | 57288591 | ID=MS.gene04393.t1.exon5;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57288591 | 57288659 | 57288591 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| chr3.4 | exon | 57284296 | 57284353 | 57284296 | ID=MS.gene04393.t1.exon6;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57284296 | 57284353 | 57284296 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| chr3.4 | exon | 57284073 | 57284172 | 57284073 | ID=MS.gene04393.t1.exon7;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57284073 | 57284172 | 57284073 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| chr3.4 | exon | 57283835 | 57283921 | 57283835 | ID=MS.gene04393.t1.exon8;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57283835 | 57283921 | 57283835 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| chr3.4 | exon | 57283648 | 57283726 | 57283648 | ID=MS.gene04393.t1.exon9;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57283648 | 57283726 | 57283648 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| chr3.4 | exon | 57283083 | 57283215 | 57283083 | ID=MS.gene04393.t1.exon10;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57283083 | 57283215 | 57283083 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| chr3.4 | exon | 57282961 | 57282995 | 57282961 | ID=MS.gene04393.t1.exon11;Parent=MS.gene04393.t1 |
| chr3.4 | CDS | 57282961 | 57282995 | 57282961 | ID=cds.MS.gene04393.t1;Parent=MS.gene04393.t1 |
| Gene Sequence |
| Protein sequence |
