Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044211.t1 | GAU51471.1 | 46.2 | 106 | 53 | 1 | 22 | 127 | 90 | 191 | 4.80E-15 | 90.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044211.t1 | A0A2Z6P4W7 | 46.2 | 106 | 53 | 1 | 22 | 127 | 90 | 191 | 3.5e-15 | 90.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044211 | MS.gene044323 | PPI |
| MS.gene70443 | MS.gene044211 | PPI |
| MS.gene044211 | MS.gene71284 | PPI |
| MS.gene044211 | MS.gene044376 | PPI |
| MS.gene044211 | MS.gene70430 | PPI |
| MS.gene044211 | MS.gene045214 | PPI |
| MS.gene044211 | MS.gene77466 | PPI |
| MS.gene044211 | MS.gene044361 | PPI |
| MS.gene044211 | MS.gene71282 | PPI |
| MS.gene044211 | MS.gene71524 | PPI |
| MS.gene044211 | MS.gene71528 | PPI |
| MS.gene044211 | MS.gene70424 | PPI |
| MS.gene044363 | MS.gene044211 | PPI |
| MS.gene044211 | MS.gene77464 | PPI |
| MS.gene77472 | MS.gene044211 | PPI |
| MS.gene70418 | MS.gene044211 | PPI |
| MS.gene044211 | MS.gene044326 | PPI |
| MS.gene044211 | MS.gene79115 | PPI |
| MS.gene044211 | MS.gene70427 | PPI |
| MS.gene044211 | MS.gene049263 | PPI |
| MS.gene044211 | MS.gene044370 | PPI |
| MS.gene044211 | MS.gene70476 | PPI |
| MS.gene044211 | MS.gene25704 | PPI |
| MS.gene044211 | MS.gene77468 | PPI |
| MS.gene044211 | MS.gene71531 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044211.t1 | MTR_3g083080 | 43.182 | 132 | 64 | 3 | 5 | 130 | 57 | 183 | 1.06e-24 | 94.0 |
| MS.gene044211.t1 | MTR_3g080680 | 42.568 | 148 | 62 | 6 | 5 | 132 | 33 | 177 | 6.63e-21 | 84.0 |
| MS.gene044211.t1 | MTR_3g083050 | 43.902 | 123 | 61 | 3 | 2 | 121 | 59 | 176 | 5.35e-20 | 81.6 |
| MS.gene044211.t1 | MTR_3g083030 | 43.902 | 123 | 61 | 3 | 2 | 121 | 82 | 199 | 6.91e-20 | 82.0 |
| MS.gene044211.t1 | MTR_3g082420 | 40.777 | 103 | 58 | 2 | 33 | 132 | 97 | 199 | 7.64e-18 | 76.3 |
| MS.gene044211.t1 | MTR_3g082340 | 40.777 | 103 | 58 | 2 | 33 | 132 | 98 | 200 | 2.27e-17 | 75.1 |
| MS.gene044211.t1 | MTR_4g055800 | 38.532 | 109 | 56 | 3 | 28 | 130 | 171 | 274 | 2.45e-14 | 68.2 |
| MS.gene044211.t1 | MTR_3g083150 | 36.232 | 138 | 75 | 5 | 4 | 131 | 57 | 191 | 2.21e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTCTGAGCTTGCTTTGA+AGG | 0.280067 | 3.3:+67852376 | MS.gene044211:CDS |
| GTCAGCAATTAGTTCTTCTA+TGG | 0.315331 | 3.3:-67852129 | None:intergenic |
| GAGAGTAGGGCGAAGATCTT+CGG | 0.406860 | 3.3:-67852348 | None:intergenic |
| GTTGTTAAGTTGTTATAGCA+TGG | 0.435461 | 3.3:-67852629 | None:intergenic |
| GTTCGACGATGACGACTTTG+CGG | 0.439069 | 3.3:+67852094 | MS.gene044211:CDS |
| GTCAAGTGTACCACTGCTAT+TGG | 0.447420 | 3.3:+67852540 | MS.gene044211:CDS |
| GGATTACAATCAAAAGAATC+AGG | 0.463360 | 3.3:+67852397 | MS.gene044211:CDS |
| GTCGTCATCGTCATCGTCCA+TGG | 0.476185 | 3.3:-67852051 | None:intergenic |
| ACTGCTATTGGTTGTGGTGG+TGG | 0.480634 | 3.3:+67852552 | MS.gene044211:CDS |
| CAGAGAGAGTAACGAGAGTA+GGG | 0.497560 | 3.3:-67852361 | None:intergenic |
| TCAGAGAGAGTAACGAGAGT+AGG | 0.497887 | 3.3:-67852362 | None:intergenic |
| CCACATAACTCTAGAACGGC+TGG | 0.510258 | 3.3:-67852302 | None:intergenic |
| TTGTTAAGTTGTTATAGCAT+GGG | 0.514294 | 3.3:-67852628 | None:intergenic |
| TGTACCACTGCTATTGGTTG+TGG | 0.522647 | 3.3:+67852546 | MS.gene044211:CDS |
| AAGATCTTCGGTTATGTCAA+AGG | 0.527327 | 3.3:-67852336 | None:intergenic |
| AGTTGTTATAGCATGGGTGT+TGG | 0.535950 | 3.3:-67852622 | None:intergenic |
| CGACTTTGCGGAAGATAAAG+AGG | 0.546436 | 3.3:+67852106 | MS.gene044211:CDS |
| AGATCTTCGGTTATGTCAAA+GGG | 0.549668 | 3.3:-67852335 | None:intergenic |
| GTTATAGCATGGGTGTTGGA+AGG | 0.561860 | 3.3:-67852618 | None:intergenic |
| ACCACTGCTATTGGTTGTGG+TGG | 0.575309 | 3.3:+67852549 | MS.gene044211:CDS |
| CCAGCCGTTCTAGAGTTATG+TGG | 0.626689 | 3.3:+67852302 | MS.gene044211:CDS |
| ACAGCCACATAACTCTAGAA+CGG | 0.642024 | 3.3:-67852306 | None:intergenic |
| TAGCAGTGGTACACTTGACA+AGG | 0.661404 | 3.3:-67852536 | None:intergenic |
| GGATGATAGATAGCATCGAA+CGG | 0.667704 | 3.3:-67852281 | None:intergenic |
| ACCACCACAACCAATAGCAG+TGG | 0.671651 | 3.3:-67852550 | None:intergenic |
| AAAGTCGTCATCGTCGAACG+TGG | 0.686780 | 3.3:-67852090 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTCTTCTTCTTAATTATTA+GGG | + | chr3.3:67852492-67852511 | MS.gene044211:intron | 20.0% |
| !! | TGAAACATGATAAAATTGAA+TGG | - | chr3.3:67852228-67852247 | None:intergenic | 20.0% |
| !! | TGTTCTTCTTCTTAATTATT+AGG | + | chr3.3:67852491-67852510 | MS.gene044211:intron | 20.0% |
| ! | CAATTATTCATCAACTAATC+TGG | + | chr3.3:67852458-67852477 | MS.gene044211:intron | 25.0% |
| AAAGATAGATTCGTACACTT+AGG | - | chr3.3:67852175-67852194 | None:intergenic | 30.0% | |
| GGATTACAATCAAAAGAATC+AGG | + | chr3.3:67852397-67852416 | MS.gene044211:CDS | 30.0% | |
| AAGATCTTCGGTTATGTCAA+AGG | - | chr3.3:67852339-67852358 | None:intergenic | 35.0% | |
| AGATCTTCGGTTATGTCAAA+GGG | - | chr3.3:67852338-67852357 | None:intergenic | 35.0% | |
| ! | GTCAGCAATTAGTTCTTCTA+TGG | - | chr3.3:67852132-67852151 | None:intergenic | 35.0% |
| !! | TTCTTCTGCTTTTGCTTGAA+AGG | - | chr3.3:67852595-67852614 | None:intergenic | 35.0% |
| AGTTGTTATAGCATGGGTGT+TGG | - | chr3.3:67852625-67852644 | None:intergenic | 40.0% | |
| ! | ACAGCCACATAACTCTAGAA+CGG | - | chr3.3:67852309-67852328 | None:intergenic | 40.0% |
| ! | GGATGATAGATAGCATCGAA+CGG | - | chr3.3:67852284-67852303 | None:intergenic | 40.0% |
| CAGAGAGAGTAACGAGAGTA+GGG | - | chr3.3:67852364-67852383 | None:intergenic | 45.0% | |
| CGACTTTGCGGAAGATAAAG+AGG | + | chr3.3:67852106-67852125 | MS.gene044211:CDS | 45.0% | |
| GTCAAGTGTACCACTGCTAT+TGG | + | chr3.3:67852540-67852559 | MS.gene044211:CDS | 45.0% | |
| TAGCAGTGGTACACTTGACA+AGG | - | chr3.3:67852539-67852558 | None:intergenic | 45.0% | |
| TCAGAGAGAGTAACGAGAGT+AGG | - | chr3.3:67852365-67852384 | None:intergenic | 45.0% | |
| ! | TCTCTCTGAGCTTGCTTTGA+AGG | + | chr3.3:67852376-67852395 | MS.gene044211:CDS | 45.0% |
| ! | TGTACCACTGCTATTGGTTG+TGG | + | chr3.3:67852546-67852565 | MS.gene044211:CDS | 45.0% |
| !! | GTTATAGCATGGGTGTTGGA+AGG | - | chr3.3:67852621-67852640 | None:intergenic | 45.0% |
| AAAGTCGTCATCGTCGAACG+TGG | - | chr3.3:67852093-67852112 | None:intergenic | 50.0% | |
| ACCACCACAACCAATAGCAG+TGG | - | chr3.3:67852553-67852572 | None:intergenic | 50.0% | |
| GAGAGTAGGGCGAAGATCTT+CGG | - | chr3.3:67852351-67852370 | None:intergenic | 50.0% | |
| GTTCGACGATGACGACTTTG+CGG | + | chr3.3:67852094-67852113 | MS.gene044211:CDS | 50.0% | |
| ! | ACCACTGCTATTGGTTGTGG+TGG | + | chr3.3:67852549-67852568 | MS.gene044211:CDS | 50.0% |
| ! | ACTGCTATTGGTTGTGGTGG+TGG | + | chr3.3:67852552-67852571 | MS.gene044211:CDS | 50.0% |
| !! | CCACATAACTCTAGAACGGC+TGG | - | chr3.3:67852305-67852324 | None:intergenic | 50.0% |
| !! | CCAGCCGTTCTAGAGTTATG+TGG | + | chr3.3:67852302-67852321 | MS.gene044211:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 67852053 | 67852647 | 67852053 | ID=MS.gene044211 |
| chr3.3 | mRNA | 67852053 | 67852647 | 67852053 | ID=MS.gene044211.t1;Parent=MS.gene044211 |
| chr3.3 | exon | 67852053 | 67852178 | 67852053 | ID=MS.gene044211.t1.exon1;Parent=MS.gene044211.t1 |
| chr3.3 | CDS | 67852053 | 67852178 | 67852053 | ID=cds.MS.gene044211.t1;Parent=MS.gene044211.t1 |
| chr3.3 | exon | 67852281 | 67852418 | 67852281 | ID=MS.gene044211.t1.exon2;Parent=MS.gene044211.t1 |
| chr3.3 | CDS | 67852281 | 67852418 | 67852281 | ID=cds.MS.gene044211.t1;Parent=MS.gene044211.t1 |
| chr3.3 | exon | 67852513 | 67852647 | 67852513 | ID=MS.gene044211.t1.exon3;Parent=MS.gene044211.t1 |
| chr3.3 | CDS | 67852513 | 67852647 | 67852513 | ID=cds.MS.gene044211.t1;Parent=MS.gene044211.t1 |
| Gene Sequence |
| Protein sequence |
