AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044361


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044361.t1 MTR_3g083150 51.452 241 83 9 1 238 3 212 5.59e-55 176
MS.gene044361.t1 MTR_3g083030 38.683 243 129 11 2 239 4 231 1.21e-37 132
MS.gene044361.t1 MTR_3g083050 37.295 244 112 11 1 239 1 208 8.54e-34 121
MS.gene044361.t1 MTR_3g083100 52.632 133 49 5 117 238 45 174 1.38e-32 117
MS.gene044361.t1 MTR_3g083080 43.182 176 89 6 63 237 40 205 2.62e-29 109
MS.gene044361.t1 MTR_3g082340 37.295 244 124 12 1 237 1 222 6.87e-29 108
MS.gene044361.t1 MTR_3g082420 37.557 221 117 12 24 237 15 221 8.08e-26 100
MS.gene044361.t1 MTR_3g080680 33.005 203 113 7 45 237 9 198 1.67e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTGTTGTAGCAGCTTTC+CGG 0.155757 3.3:+69738960 None:intergenic
TTGGGTTATGGGAAAGGGTC+TGG 0.210252 3.3:+69738495 None:intergenic
AGGGATACGTTGTTGGGTTA+TGG 0.235089 3.3:+69738483 None:intergenic
TCCGAATCCGAGTCAGATTC+TGG 0.250389 3.3:+69738906 None:intergenic
CTCTGTTGTAGCAGCTTTCC+GGG 0.261650 3.3:+69738961 None:intergenic
GTATTTGCTGCGCGTGGAAT+AGG 0.277573 3.3:+69738535 None:intergenic
GGGATACGTTGTTGGGTTAT+GGG 0.293781 3.3:+69738484 None:intergenic
TTATTCTCATAAACAAATTT+CGG 0.296115 3.3:+69738732 None:intergenic
AAGCTGGACAAACTCATATT+TGG 0.298094 3.3:+69738315 None:intergenic
TCTCGTTCCGCTTCCCGTTT+CGG 0.333023 3.3:+69738930 None:intergenic
TACCATGGTGCGTTAATTAG+AGG 0.349376 3.3:+69738117 None:intergenic
ACGCCTCTCCAAACTTGCTT+TGG 0.354815 3.3:-69738452 MS.gene044361:CDS
TGGAGAGGCGTTTGAGAAGA+GGG 0.361494 3.3:+69738464 None:intergenic
GGTTCAACAACTTGAGGTAT+TGG 0.363055 3.3:+69738229 None:intergenic
AAAATACAATGTTGACAATC+AGG 0.364241 3.3:-69738428 MS.gene044361:intron
ATCGAAAGTGAAAGTGGAAT+TGG 0.367090 3.3:-69738853 MS.gene044361:CDS
TCGACCGAGACTCTCGTGTT+TGG 0.383497 3.3:-69739002 MS.gene044361:CDS
TGGGTTATGGGAAAGGGTCT+GGG 0.394715 3.3:+69738496 None:intergenic
GAGAAGAGGGATACGTTGTT+GGG 0.411423 3.3:+69738477 None:intergenic
TAATAAGTACCAAGAGGAAT+AGG 0.423275 3.3:+69738274 None:intergenic
CTCGGATTCGGAATCGGAAT+CGG 0.425215 3.3:-69738895 MS.gene044361:CDS
ATCTGACTCGGATTCGGAAT+CGG 0.428389 3.3:-69738901 MS.gene044361:CDS
ACCAGAATCTGACTCGGATT+CGG 0.435178 3.3:-69738907 MS.gene044361:CDS
CAGAGGGAAGTGGCCGAAAC+GGG 0.441096 3.3:-69738943 MS.gene044361:CDS
TGAGAAGAGGGATACGTTGT+TGG 0.443257 3.3:+69738476 None:intergenic
CTCTCCTTCAGATCTTCTAA+TGG 0.445268 3.3:+69738807 None:intergenic
GCCTGAAAAGTTGTTGTAGC+AGG 0.449700 3.3:+69738196 None:intergenic
ACCCGTTCTCCTATTCCTCT+TGG 0.450300 3.3:-69738283 MS.gene044361:CDS
CATGTCTGGAACAAGATATC+TGG 0.451078 3.3:-69738172 MS.gene044361:CDS
AGTTGTTGTAGCAGGGGTGT+TGG 0.460802 3.3:+69738204 None:intergenic
TATTTGCTGCGCGTGGAATA+GGG 0.467085 3.3:+69738536 None:intergenic
TTGGAGAGGCGTTTGAGAAG+AGG 0.471987 3.3:+69738463 None:intergenic
AACGGGTTGCCTTGACAAGC+TGG 0.476282 3.3:+69738299 None:intergenic
AAGTGGAATTGGACCCGTTA+TGG 0.482799 3.3:-69738842 MS.gene044361:CDS
AAAATGCTGTCGAAAAGTAT+TGG 0.492089 3.3:-69738680 MS.gene044361:CDS
GTATCCGTTCCCACCATAAC+GGG 0.494522 3.3:+69738829 None:intergenic
ACCAAGAGGAATAGGAGAAC+GGG 0.497967 3.3:+69738282 None:intergenic
CAAGATATCTGGAACAACTA+AGG 0.499344 3.3:-69738161 MS.gene044361:CDS
CAGATATCTTGTTCCAGACA+TGG 0.500861 3.3:+69738173 None:intergenic
ACGTTGTTGGGTTATGGGAA+AGG 0.502825 3.3:+69738489 None:intergenic
AGCCGCCATCCAACCTCCCC+CGG 0.506131 3.3:-69738979 MS.gene044361:CDS
ACAGAGGGAAGTGGCCGAAA+CGG 0.508391 3.3:-69738944 MS.gene044361:CDS
ATCGGAATCGGAATCAGAAT+CGG 0.511451 3.3:-69738883 MS.gene044361:CDS
GTTGTAGCAGGGGTGTTGGA+AGG 0.514159 3.3:+69738208 None:intergenic
CGTTGTTGGGTTATGGGAAA+GGG 0.515504 3.3:+69738490 None:intergenic
GAAGTGGCCGAAACGGGAAG+CGG 0.529767 3.3:-69738937 MS.gene044361:CDS
GGTATCCGTTCCCACCATAA+CGG 0.532487 3.3:+69738828 None:intergenic
AGTAGAATCGAAAGTGAAAG+TGG 0.543261 3.3:-69738859 MS.gene044361:CDS
TGGAATAGGGATGGCATCGA+AGG 0.543328 3.3:+69738549 None:intergenic
AGATATCTTGTTCCAGACAT+GGG 0.545976 3.3:+69738174 None:intergenic
GAAAAGGATCATCTTCATAA+CGG 0.546737 3.3:+69738772 None:intergenic
GGCTCCAAACACGAGAGTCT+CGG 0.559644 3.3:+69738998 None:intergenic
TGGAATTGGACCCGTTATGG+TGG 0.566427 3.3:-69738839 MS.gene044361:CDS
TCTGTTGTAGCAGCTTTCCG+GGG 0.568607 3.3:+69738962 None:intergenic
TACCAAGAGGAATAGGAGAA+CGG 0.570966 3.3:+69738281 None:intergenic
TGCTGTCGAAAAGTATTGGA+AGG 0.572056 3.3:-69738676 MS.gene044361:CDS
GATACCATTAGAAGATCTGA+AGG 0.573494 3.3:-69738811 MS.gene044361:CDS
TTGGAAGGTTCAACAACTTG+AGG 0.575752 3.3:+69738223 None:intergenic
TGGACCCGTTATGGTGGGAA+CGG 0.578043 3.3:-69738833 MS.gene044361:CDS
CGGAAAGCTGCTACAACAGA+GGG 0.584202 3.3:-69738959 MS.gene044361:CDS
TGAGTTTGTCCAGCTTGTCA+AGG 0.586521 3.3:-69738308 MS.gene044361:CDS
CCGGAAAGCTGCTACAACAG+AGG 0.589302 3.3:-69738960 MS.gene044361:CDS
CCTGAAAAGTTGTTGTAGCA+GGG 0.590814 3.3:+69738197 None:intergenic
CTGAAAAGTTGTTGTAGCAG+GGG 0.591211 3.3:+69738198 None:intergenic
GCTGCTACAACAGAGGGAAG+TGG 0.596216 3.3:-69738953 MS.gene044361:CDS
CCACATGTATTTGCTGCGCG+TGG 0.600455 3.3:+69738529 None:intergenic
TTTCCAAAGCAAGTTTGGAG+AGG 0.618640 3.3:+69738449 None:intergenic
TGCTGCGCGTGGAATAGGGA+TGG 0.619194 3.3:+69738540 None:intergenic
AGCTATGAGAGATAAAGAAG+AGG 0.620914 3.3:-69738709 MS.gene044361:CDS
GGAATAGGGATGGCATCGAA+GGG 0.630858 3.3:+69738550 None:intergenic
GGAATTGGACCCGTTATGGT+GGG 0.637797 3.3:-69738838 MS.gene044361:CDS
CTGTTGTAGCAGCTTTCCGG+GGG 0.645513 3.3:+69738963 None:intergenic
ACGAGAACCAGAATCTGACT+CGG 0.655126 3.3:-69738913 MS.gene044361:CDS
GTAATGTAATAAGTACCAAG+AGG 0.663480 3.3:+69738268 None:intergenic
CCACGCGCAGCAAATACATG+TGG 0.685685 3.3:-69738529 MS.gene044361:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAACTAAAATTAAGAG+AGG + chr3.3:69738513-69738532 None:intergenic 15.0%
!! GATATAAACAAAAACAATAT+TGG + chr3.3:69738750-69738769 None:intergenic 15.0%
!! TTAAAACTAAAATTAAGAGA+GGG + chr3.3:69738512-69738531 None:intergenic 15.0%
!! TTATTCTCATAAACAAATTT+CGG + chr3.3:69738405-69738424 None:intergenic 15.0%
!!! TACTTATTACATTACTTTTA+AGG - chr3.3:69738874-69738893 MS.gene044361:CDS 15.0%
! AAAATACAATGTTGACAATC+AGG - chr3.3:69738706-69738725 MS.gene044361:CDS 25.0%
ATTTGGCCTAAATAAGAGAA+AGG + chr3.3:69738805-69738824 None:intergenic 30.0%
CACGTAAAAAAGTAAGGAAA+AGG + chr3.3:69738381-69738400 None:intergenic 30.0%
GAAAAGGATCATCTTCATAA+CGG + chr3.3:69738365-69738384 None:intergenic 30.0%
GTAATGTAATAAGTACCAAG+AGG + chr3.3:69738869-69738888 None:intergenic 30.0%
TAATAAGTACCAAGAGGAAT+AGG + chr3.3:69738863-69738882 None:intergenic 30.0%
! AAAATGCTGTCGAAAAGTAT+TGG - chr3.3:69738454-69738473 MS.gene044361:CDS 30.0%
! GTATTTTTCCAAAGCAAGTT+TGG + chr3.3:69738693-69738712 None:intergenic 30.0%
! GTTCTTCCTTTCTCTTATTT+AGG - chr3.3:69738796-69738815 MS.gene044361:CDS 30.0%
AAGCTGGACAAACTCATATT+TGG + chr3.3:69738822-69738841 None:intergenic 35.0%
AGAAAGGAAGAACAATGAAG+CGG + chr3.3:69738789-69738808 None:intergenic 35.0%
AGATATCTTGTTCCAGACAT+GGG + chr3.3:69738963-69738982 None:intergenic 35.0%
AGCTATGAGAGATAAAGAAG+AGG - chr3.3:69738425-69738444 MS.gene044361:intron 35.0%
AGTAGAATCGAAAGTGAAAG+TGG - chr3.3:69738275-69738294 MS.gene044361:CDS 35.0%
ATCGAAAGTGAAAGTGGAAT+TGG - chr3.3:69738281-69738300 MS.gene044361:CDS 35.0%
GATACCATTAGAAGATCTGA+AGG - chr3.3:69738323-69738342 MS.gene044361:intron 35.0%
TTCGGACACGTAAAAAAGTA+AGG + chr3.3:69738387-69738406 None:intergenic 35.0%
! CAAGATATCTGGAACAACTA+AGG - chr3.3:69738973-69738992 MS.gene044361:CDS 35.0%
ATCGGAATCGGAATCAGAAT+CGG - chr3.3:69738251-69738270 MS.gene044361:CDS 40.0%
CAGATATCTTGTTCCAGACA+TGG + chr3.3:69738964-69738983 None:intergenic 40.0%
CCTGAAAAGTTGTTGTAGCA+GGG + chr3.3:69738940-69738959 None:intergenic 40.0%
CTCTCCTTCAGATCTTCTAA+TGG + chr3.3:69738330-69738349 None:intergenic 40.0%
CTGAAAAGTTGTTGTAGCAG+GGG + chr3.3:69738939-69738958 None:intergenic 40.0%
GGTTCAACAACTTGAGGTAT+TGG + chr3.3:69738908-69738927 None:intergenic 40.0%
TACCAAGAGGAATAGGAGAA+CGG + chr3.3:69738856-69738875 None:intergenic 40.0%
TTGGAAGGTTCAACAACTTG+AGG + chr3.3:69738914-69738933 None:intergenic 40.0%
TTTCCAAAGCAAGTTTGGAG+AGG + chr3.3:69738688-69738707 None:intergenic 40.0%
! CATGTCTGGAACAAGATATC+TGG - chr3.3:69738962-69738981 MS.gene044361:CDS 40.0%
!! TGCTGTCGAAAAGTATTGGA+AGG - chr3.3:69738458-69738477 MS.gene044361:CDS 40.0%
AAGTGGAATTGGACCCGTTA+TGG - chr3.3:69738292-69738311 MS.gene044361:CDS 45.0%
ACCAAGAGGAATAGGAGAAC+GGG + chr3.3:69738855-69738874 None:intergenic 45.0%
ACGAGAACCAGAATCTGACT+CGG - chr3.3:69738221-69738240 MS.gene044361:CDS 45.0%
ACGTTGTTGGGTTATGGGAA+AGG + chr3.3:69738648-69738667 None:intergenic 45.0%
AGGGATACGTTGTTGGGTTA+TGG + chr3.3:69738654-69738673 None:intergenic 45.0%
CGTTGTTGGGTTATGGGAAA+GGG + chr3.3:69738647-69738666 None:intergenic 45.0%
GAGAAGAGGGATACGTTGTT+GGG + chr3.3:69738660-69738679 None:intergenic 45.0%
GCCTGAAAAGTTGTTGTAGC+AGG + chr3.3:69738941-69738960 None:intergenic 45.0%
GGGATACGTTGTTGGGTTAT+GGG + chr3.3:69738653-69738672 None:intergenic 45.0%
TGAGAAGAGGGATACGTTGT+TGG + chr3.3:69738661-69738680 None:intergenic 45.0%
TGAGTTTGTCCAGCTTGTCA+AGG - chr3.3:69738826-69738845 MS.gene044361:CDS 45.0%
! CCCTGCTACAACAACTTTTC+AGG - chr3.3:69738937-69738956 MS.gene044361:CDS 45.0%
! TATTTGCTGCGCGTGGAATA+GGG + chr3.3:69738601-69738620 None:intergenic 45.0%
!! ACCAGAATCTGACTCGGATT+CGG - chr3.3:69738227-69738246 MS.gene044361:CDS 45.0%
!! ATCTGACTCGGATTCGGAAT+CGG - chr3.3:69738233-69738252 MS.gene044361:CDS 45.0%
ACCCGTTCTCCTATTCCTCT+TGG - chr3.3:69738851-69738870 MS.gene044361:CDS 50.0%
CCTCTGTTGTAGCAGCTTTC+CGG + chr3.3:69738177-69738196 None:intergenic 50.0%
CGGAAAGCTGCTACAACAGA+GGG - chr3.3:69738175-69738194 MS.gene044361:CDS 50.0%
CTCGGATTCGGAATCGGAAT+CGG - chr3.3:69738239-69738258 MS.gene044361:CDS 50.0%
CTCTGTTGTAGCAGCTTTCC+GGG + chr3.3:69738176-69738195 None:intergenic 50.0%
GGAATTGGACCCGTTATGGT+GGG - chr3.3:69738296-69738315 MS.gene044361:CDS 50.0%
GGTATCCGTTCCCACCATAA+CGG + chr3.3:69738309-69738328 None:intergenic 50.0%
GTATCCGTTCCCACCATAAC+GGG + chr3.3:69738308-69738327 None:intergenic 50.0%
TCCGAATCCGAGTCAGATTC+TGG + chr3.3:69738231-69738250 None:intergenic 50.0%
TCTGTTGTAGCAGCTTTCCG+GGG + chr3.3:69738175-69738194 None:intergenic 50.0%
TGGAATTGGACCCGTTATGG+TGG - chr3.3:69738295-69738314 MS.gene044361:CDS 50.0%
TGGGTTATGGGAAAGGGTCT+GGG + chr3.3:69738641-69738660 None:intergenic 50.0%
TTGGGTTATGGGAAAGGGTC+TGG + chr3.3:69738642-69738661 None:intergenic 50.0%
! ACGCCTCTCCAAACTTGCTT+TGG - chr3.3:69738682-69738701 MS.gene044361:CDS 50.0%
! AGTTGTTGTAGCAGGGGTGT+TGG + chr3.3:69738933-69738952 None:intergenic 50.0%
! GGAATAGGGATGGCATCGAA+GGG + chr3.3:69738587-69738606 None:intergenic 50.0%
! GTATTTGCTGCGCGTGGAAT+AGG + chr3.3:69738602-69738621 None:intergenic 50.0%
! TGGAATAGGGATGGCATCGA+AGG + chr3.3:69738588-69738607 None:intergenic 50.0%
!! CAACTTTTCAGGCCCATGTC+TGG - chr3.3:69738948-69738967 MS.gene044361:CDS 50.0%
!! TGGAGAGGCGTTTGAGAAGA+GGG + chr3.3:69738673-69738692 None:intergenic 50.0%
!! TTGGAGAGGCGTTTGAGAAG+AGG + chr3.3:69738674-69738693 None:intergenic 50.0%
ACAGAGGGAAGTGGCCGAAA+CGG - chr3.3:69738190-69738209 MS.gene044361:CDS 55.0%
CCACGCGCAGCAAATACATG+TGG - chr3.3:69738605-69738624 MS.gene044361:intron 55.0%
CCGGAAAGCTGCTACAACAG+AGG - chr3.3:69738174-69738193 MS.gene044361:CDS 55.0%
CTGTTGTAGCAGCTTTCCGG+GGG + chr3.3:69738174-69738193 None:intergenic 55.0%
GCTGCTACAACAGAGGGAAG+TGG - chr3.3:69738181-69738200 MS.gene044361:CDS 55.0%
GGCTCCAAACACGAGAGTCT+CGG + chr3.3:69738139-69738158 None:intergenic 55.0%
TCGACCGAGACTCTCGTGTT+TGG - chr3.3:69738132-69738151 MS.gene044361:CDS 55.0%
TCTCGTTCCGCTTCCCGTTT+CGG + chr3.3:69738207-69738226 None:intergenic 55.0%
! CCACATGTATTTGCTGCGCG+TGG + chr3.3:69738608-69738627 None:intergenic 55.0%
! TGGACCCGTTATGGTGGGAA+CGG - chr3.3:69738301-69738320 MS.gene044361:CDS 55.0%
!! AACGGGTTGCCTTGACAAGC+TGG + chr3.3:69738838-69738857 None:intergenic 55.0%
!! GTTGTAGCAGGGGTGTTGGA+AGG + chr3.3:69738929-69738948 None:intergenic 55.0%
CAGAGGGAAGTGGCCGAAAC+GGG - chr3.3:69738191-69738210 MS.gene044361:CDS 60.0%
GAAGTGGCCGAAACGGGAAG+CGG - chr3.3:69738197-69738216 MS.gene044361:CDS 60.0%
TGCTGCGCGTGGAATAGGGA+TGG + chr3.3:69738597-69738616 None:intergenic 60.0%
TTGTAGCAGCTTTCCGGGGG+AGG + chr3.3:69738171-69738190 None:intergenic 60.0%
AGCAGCTTTCCGGGGGAGGT+TGG + chr3.3:69738167-69738186 None:intergenic 65.0%
GCTTTCCGGGGGAGGTTGGA+TGG + chr3.3:69738163-69738182 None:intergenic 65.0%
TTCCGGGGGAGGTTGGATGG+CGG + chr3.3:69738160-69738179 None:intergenic 65.0%
AGCCGCCATCCAACCTCCCC+CGG - chr3.3:69738155-69738174 MS.gene044361:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 69738129 69739027 69738129 ID=MS.gene044361
chr3.3 mRNA 69738129 69739027 69738129 ID=MS.gene044361.t1;Parent=MS.gene044361
chr3.3 exon 69738659 69739027 69738659 ID=MS.gene044361.t1.exon1;Parent=MS.gene044361.t1
chr3.3 CDS 69738659 69739027 69738659 ID=cds.MS.gene044361.t1;Parent=MS.gene044361.t1
chr3.3 exon 69738429 69738572 69738429 ID=MS.gene044361.t1.exon2;Parent=MS.gene044361.t1
chr3.3 CDS 69738429 69738572 69738429 ID=cds.MS.gene044361.t1;Parent=MS.gene044361.t1
chr3.3 exon 69738129 69738338 69738129 ID=MS.gene044361.t1.exon3;Parent=MS.gene044361.t1
chr3.3 CDS 69738129 69738338 69738129 ID=cds.MS.gene044361.t1;Parent=MS.gene044361.t1
Gene Sequence

>MS.gene044361

ATGTCGACCGAGACTCTCGTGTTTGGAGCCGCCATCCAACCTCCCCCGGAAAGCTGCTACAACAGAGGGAAGTGGCCGAAACGGGAAGCGGAACGAGAACCAGAATCTGACTCGGATTCGGAATCGGAATCGGAATCAGAATCGGAAGTAGAATCGAAAGTGAAAGTGGAATTGGACCCGTTATGGTGGGAACGGATACCATTAGAAGATCTGAAGGAGAGTGATTTCGCTAGCCGTTATGAAGATGATCCTTTTCCTTACTTTTTTACGTGTCCGAAATTTGTTTATGAGAATAAAGCTATGAGAGATAAAGAAGAGGCTAGAAAAAATGCTGTCGAAAAGTATTGGAAGGAAGCTGAGAACATAAGTGTATGTTGTTTCCCTCTCTTAATTTTAGTTTTAATTTTGTATTTAACTCTTTTAATCTGTTTCTGTTTCTGTACTCTGCTGCACAGCCCTTCGATGCCATCCCTATTCCACGCGCAGCAAATACATGTGGCAATAACTATCCCAGACCCTTTCCCATAACCCAACAACGTATCCCTCTTCTCAAACGCCTCTCCAAACTTGCTTTGGAAAAATACAATGTTGACAATCAGGTTTTAAACTACTACTTCACCAATATTGTTTTTGTTTATATCTTATTCTTTAGTTCATCCGCTTCATTGTTCTTCCTTTCTCTTATTTAGGCCAAATATGAGTTTGTCCAGCTTGTCAAGGCAACCCGTTCTCCTATTCCTCTTGGTACTTATTACATTACTTTTAAGGCAAAACAACCAATACCTCAAGTTGTTGAACCTTCCAACACCCCTGCTACAACAACTTTTCAGGCCCATGTCTGGAACAAGATATCTGGAACAACTAAGGTCAAGTCGTGTTCCATTAAAACCTCTAATTAA

Protein sequence

>MS.gene044361.t1

MSTETLVFGAAIQPPPESCYNRGKWPKREAEREPESDSDSESESESESEVESKVKVELDPLWWERIPLEDLKESDFASRYEDDPFPYFFTCPKFVYENKAMRDKEEARKNAVEKYWKEAENISPFDAIPIPRAANTCGNNYPRPFPITQQRIPLLKRLSKLALEKYNVDNQAKYEFVQLVKATRSPIPLGTYYITFKAKQPIPQVVEPSNTPATTTFQAHVWNKISGTTKVKSCSIKTSN