AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71284


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71284.t1 MTR_3g083150 51.452 241 83 9 1 238 3 212 5.59e-55 176
MS.gene71284.t1 MTR_3g083030 38.683 243 129 11 2 239 4 231 1.21e-37 132
MS.gene71284.t1 MTR_3g083050 37.295 244 112 11 1 239 1 208 8.54e-34 121
MS.gene71284.t1 MTR_3g083100 52.632 133 49 5 117 238 45 174 1.38e-32 117
MS.gene71284.t1 MTR_3g083080 43.182 176 89 6 63 237 40 205 2.62e-29 109
MS.gene71284.t1 MTR_3g082340 37.295 244 124 12 1 237 1 222 6.87e-29 108
MS.gene71284.t1 MTR_3g082420 37.557 221 117 12 24 237 15 221 8.08e-26 100
MS.gene71284.t1 MTR_3g080680 33.005 203 113 7 45 237 9 198 1.67e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTGTTGTAGCAGCTTTC+CGG 0.155757 3.4:+78169301 None:intergenic
TTGGGTTATGGGAAAGGGTC+TGG 0.210252 3.4:+78168836 None:intergenic
AGGGATACGTTGTTGGGTTA+TGG 0.235089 3.4:+78168824 None:intergenic
TCCGAATCCGAGTCAGATTC+TGG 0.250389 3.4:+78169247 None:intergenic
CTCTGTTGTAGCAGCTTTCC+GGG 0.261650 3.4:+78169302 None:intergenic
GTATTTGCTGCGCGTGGAAT+AGG 0.277573 3.4:+78168876 None:intergenic
GGGATACGTTGTTGGGTTAT+GGG 0.293781 3.4:+78168825 None:intergenic
TTATTCTCATAAACAAATTT+CGG 0.296115 3.4:+78169073 None:intergenic
AAGCTGGACAAACTCATATT+TGG 0.298094 3.4:+78168656 None:intergenic
TCTCGTTCCGCTTCCCGTTT+CGG 0.333023 3.4:+78169271 None:intergenic
TACCATGGTGCGTTAATTAG+AGG 0.349376 3.4:+78168458 None:intergenic
ACGCCTCTCCAAACTTGCTT+TGG 0.354815 3.4:-78168793 MS.gene71284:CDS
TGGAGAGGCGTTTGAGAAGA+GGG 0.361494 3.4:+78168805 None:intergenic
GGTTCAACAACTTGAGGTAT+TGG 0.363055 3.4:+78168570 None:intergenic
AAAATACAATGTTGACAATC+AGG 0.364241 3.4:-78168769 MS.gene71284:intron
ATCGAAAGTGAAAGTGGAAT+TGG 0.367090 3.4:-78169194 MS.gene71284:CDS
TCGACCGAGACTCTCGTGTT+TGG 0.383497 3.4:-78169343 MS.gene71284:CDS
TGGGTTATGGGAAAGGGTCT+GGG 0.394715 3.4:+78168837 None:intergenic
GAGAAGAGGGATACGTTGTT+GGG 0.411423 3.4:+78168818 None:intergenic
TAATAAGTACCAAGAGGAAT+AGG 0.423275 3.4:+78168615 None:intergenic
CTCGGATTCGGAATCGGAAT+CGG 0.425215 3.4:-78169236 MS.gene71284:CDS
ATCTGACTCGGATTCGGAAT+CGG 0.428389 3.4:-78169242 MS.gene71284:CDS
ACCAGAATCTGACTCGGATT+CGG 0.435178 3.4:-78169248 MS.gene71284:CDS
CAGAGGGAAGTGGCCGAAAC+GGG 0.441096 3.4:-78169284 MS.gene71284:CDS
TGAGAAGAGGGATACGTTGT+TGG 0.443257 3.4:+78168817 None:intergenic
CTCTCCTTCAGATCTTCTAA+TGG 0.445268 3.4:+78169148 None:intergenic
GCCTGAAAAGTTGTTGTAGC+AGG 0.449700 3.4:+78168537 None:intergenic
ACCCGTTCTCCTATTCCTCT+TGG 0.450300 3.4:-78168624 MS.gene71284:CDS
CATGTCTGGAACAAGATATC+TGG 0.451078 3.4:-78168513 MS.gene71284:CDS
AGTTGTTGTAGCAGGGGTGT+TGG 0.460802 3.4:+78168545 None:intergenic
TATTTGCTGCGCGTGGAATA+GGG 0.467085 3.4:+78168877 None:intergenic
TTGGAGAGGCGTTTGAGAAG+AGG 0.471987 3.4:+78168804 None:intergenic
AACGGGTTGCCTTGACAAGC+TGG 0.476282 3.4:+78168640 None:intergenic
AAGTGGAATTGGACCCGTTA+TGG 0.482799 3.4:-78169183 MS.gene71284:CDS
AAAATGCTGTCGAAAAGTAT+TGG 0.492089 3.4:-78169021 MS.gene71284:CDS
GTATCCGTTCCCACCATAAC+GGG 0.494522 3.4:+78169170 None:intergenic
ACCAAGAGGAATAGGAGAAC+GGG 0.497967 3.4:+78168623 None:intergenic
CAAGATATCTGGAACAACTA+AGG 0.499344 3.4:-78168502 MS.gene71284:CDS
CAGATATCTTGTTCCAGACA+TGG 0.500861 3.4:+78168514 None:intergenic
ACGTTGTTGGGTTATGGGAA+AGG 0.502825 3.4:+78168830 None:intergenic
AGCCGCCATCCAACCTCCCC+CGG 0.506131 3.4:-78169320 MS.gene71284:CDS
ACAGAGGGAAGTGGCCGAAA+CGG 0.508391 3.4:-78169285 MS.gene71284:CDS
ATCGGAATCGGAATCAGAAT+CGG 0.511451 3.4:-78169224 MS.gene71284:CDS
GTTGTAGCAGGGGTGTTGGA+AGG 0.514159 3.4:+78168549 None:intergenic
CGTTGTTGGGTTATGGGAAA+GGG 0.515504 3.4:+78168831 None:intergenic
GAAGTGGCCGAAACGGGAAG+CGG 0.529767 3.4:-78169278 MS.gene71284:CDS
GGTATCCGTTCCCACCATAA+CGG 0.532487 3.4:+78169169 None:intergenic
AGTAGAATCGAAAGTGAAAG+TGG 0.543261 3.4:-78169200 MS.gene71284:CDS
TGGAATAGGGATGGCATCGA+AGG 0.543328 3.4:+78168890 None:intergenic
AGATATCTTGTTCCAGACAT+GGG 0.545976 3.4:+78168515 None:intergenic
GAAAAGGATCATCTTCATAA+CGG 0.546737 3.4:+78169113 None:intergenic
GGCTCCAAACACGAGAGTCT+CGG 0.559644 3.4:+78169339 None:intergenic
TGGAATTGGACCCGTTATGG+TGG 0.566427 3.4:-78169180 MS.gene71284:CDS
TCTGTTGTAGCAGCTTTCCG+GGG 0.568607 3.4:+78169303 None:intergenic
TACCAAGAGGAATAGGAGAA+CGG 0.570966 3.4:+78168622 None:intergenic
TGCTGTCGAAAAGTATTGGA+AGG 0.572056 3.4:-78169017 MS.gene71284:CDS
GATACCATTAGAAGATCTGA+AGG 0.573494 3.4:-78169152 MS.gene71284:CDS
TTGGAAGGTTCAACAACTTG+AGG 0.575752 3.4:+78168564 None:intergenic
TGGACCCGTTATGGTGGGAA+CGG 0.578043 3.4:-78169174 MS.gene71284:CDS
CGGAAAGCTGCTACAACAGA+GGG 0.584202 3.4:-78169300 MS.gene71284:CDS
TGAGTTTGTCCAGCTTGTCA+AGG 0.586521 3.4:-78168649 MS.gene71284:CDS
CCGGAAAGCTGCTACAACAG+AGG 0.589302 3.4:-78169301 MS.gene71284:CDS
CCTGAAAAGTTGTTGTAGCA+GGG 0.590814 3.4:+78168538 None:intergenic
CTGAAAAGTTGTTGTAGCAG+GGG 0.591211 3.4:+78168539 None:intergenic
GCTGCTACAACAGAGGGAAG+TGG 0.596216 3.4:-78169294 MS.gene71284:CDS
CCACATGTATTTGCTGCGCG+TGG 0.600455 3.4:+78168870 None:intergenic
TTTCCAAAGCAAGTTTGGAG+AGG 0.618640 3.4:+78168790 None:intergenic
TGCTGCGCGTGGAATAGGGA+TGG 0.619194 3.4:+78168881 None:intergenic
AGCTATGAGAGATAAAGAAG+AGG 0.620914 3.4:-78169050 MS.gene71284:CDS
GGAATAGGGATGGCATCGAA+GGG 0.630858 3.4:+78168891 None:intergenic
GGAATTGGACCCGTTATGGT+GGG 0.637797 3.4:-78169179 MS.gene71284:CDS
CTGTTGTAGCAGCTTTCCGG+GGG 0.645513 3.4:+78169304 None:intergenic
ACGAGAACCAGAATCTGACT+CGG 0.655126 3.4:-78169254 MS.gene71284:CDS
GTAATGTAATAAGTACCAAG+AGG 0.663480 3.4:+78168609 None:intergenic
CCACGCGCAGCAAATACATG+TGG 0.685685 3.4:-78168870 MS.gene71284:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAACAAAAATTAAGAG+AGG + chr3.4:78168854-78168873 None:intergenic 15.0%
!! GATATAAACAAAAACAATAT+TGG + chr3.4:78169091-78169110 None:intergenic 15.0%
!! TTAAAACAAAAATTAAGAGA+GGG + chr3.4:78168853-78168872 None:intergenic 15.0%
!! TTATTCTCATAAACAAATTT+CGG + chr3.4:78168746-78168765 None:intergenic 15.0%
!!! TACTTATTACATTACTTTTA+AGG - chr3.4:78169215-78169234 MS.gene71284:CDS 15.0%
! AAAATACAATGTTGACAATC+AGG - chr3.4:78169047-78169066 MS.gene71284:CDS 25.0%
ATTTGGCCTAAATAAGAGAA+AGG + chr3.4:78169146-78169165 None:intergenic 30.0%
CACGTAAAAAAGTAAGGAAA+AGG + chr3.4:78168722-78168741 None:intergenic 30.0%
GAAAAGGATCATCTTCATAA+CGG + chr3.4:78168706-78168725 None:intergenic 30.0%
GTAATGTAATAAGTACCAAG+AGG + chr3.4:78169210-78169229 None:intergenic 30.0%
TAATAAGTACCAAGAGGAAT+AGG + chr3.4:78169204-78169223 None:intergenic 30.0%
! AAAATGCTGTCGAAAAGTAT+TGG - chr3.4:78168795-78168814 MS.gene71284:CDS 30.0%
! GTATTTTTCCAAAGCAAGTT+TGG + chr3.4:78169034-78169053 None:intergenic 30.0%
! GTTCTTCCTTTCTCTTATTT+AGG - chr3.4:78169137-78169156 MS.gene71284:CDS 30.0%
AAGCTGGACAAACTCATATT+TGG + chr3.4:78169163-78169182 None:intergenic 35.0%
AGAAAGGAAGAACAATGAAG+CGG + chr3.4:78169130-78169149 None:intergenic 35.0%
AGATATCTTGTTCCAGACAT+GGG + chr3.4:78169304-78169323 None:intergenic 35.0%
AGCTATGAGAGATAAAGAAG+AGG - chr3.4:78168766-78168785 MS.gene71284:intron 35.0%
AGTAGAATCGAAAGTGAAAG+TGG - chr3.4:78168616-78168635 MS.gene71284:CDS 35.0%
ATCGAAAGTGAAAGTGGAAT+TGG - chr3.4:78168622-78168641 MS.gene71284:CDS 35.0%
GATACCATTAGAAGATCTGA+AGG - chr3.4:78168664-78168683 MS.gene71284:intron 35.0%
TTCGGACACGTAAAAAAGTA+AGG + chr3.4:78168728-78168747 None:intergenic 35.0%
! CAAGATATCTGGAACAACTA+AGG - chr3.4:78169314-78169333 MS.gene71284:CDS 35.0%
ATCGGAATCGGAATCAGAAT+CGG - chr3.4:78168592-78168611 MS.gene71284:CDS 40.0%
CAGATATCTTGTTCCAGACA+TGG + chr3.4:78169305-78169324 None:intergenic 40.0%
CCTGAAAAGTTGTTGTAGCA+GGG + chr3.4:78169281-78169300 None:intergenic 40.0%
CTCTCCTTCAGATCTTCTAA+TGG + chr3.4:78168671-78168690 None:intergenic 40.0%
CTGAAAAGTTGTTGTAGCAG+GGG + chr3.4:78169280-78169299 None:intergenic 40.0%
GGTTCAACAACTTGAGGTAT+TGG + chr3.4:78169249-78169268 None:intergenic 40.0%
TACCAAGAGGAATAGGAGAA+CGG + chr3.4:78169197-78169216 None:intergenic 40.0%
TTGGAAGGTTCAACAACTTG+AGG + chr3.4:78169255-78169274 None:intergenic 40.0%
TTTCCAAAGCAAGTTTGGAG+AGG + chr3.4:78169029-78169048 None:intergenic 40.0%
! CATGTCTGGAACAAGATATC+TGG - chr3.4:78169303-78169322 MS.gene71284:CDS 40.0%
!! TGCTGTCGAAAAGTATTGGA+AGG - chr3.4:78168799-78168818 MS.gene71284:CDS 40.0%
AAGTGGAATTGGACCCGTTA+TGG - chr3.4:78168633-78168652 MS.gene71284:CDS 45.0%
ACCAAGAGGAATAGGAGAAC+GGG + chr3.4:78169196-78169215 None:intergenic 45.0%
ACGAGAACCAGAATCTGACT+CGG - chr3.4:78168562-78168581 MS.gene71284:CDS 45.0%
ACGTTGTTGGGTTATGGGAA+AGG + chr3.4:78168989-78169008 None:intergenic 45.0%
AGGGATACGTTGTTGGGTTA+TGG + chr3.4:78168995-78169014 None:intergenic 45.0%
CGTTGTTGGGTTATGGGAAA+GGG + chr3.4:78168988-78169007 None:intergenic 45.0%
GAGAAGAGGGATACGTTGTT+GGG + chr3.4:78169001-78169020 None:intergenic 45.0%
GCCTGAAAAGTTGTTGTAGC+AGG + chr3.4:78169282-78169301 None:intergenic 45.0%
GGGATACGTTGTTGGGTTAT+GGG + chr3.4:78168994-78169013 None:intergenic 45.0%
TGAGAAGAGGGATACGTTGT+TGG + chr3.4:78169002-78169021 None:intergenic 45.0%
TGAGTTTGTCCAGCTTGTCA+AGG - chr3.4:78169167-78169186 MS.gene71284:CDS 45.0%
! CCCTGCTACAACAACTTTTC+AGG - chr3.4:78169278-78169297 MS.gene71284:CDS 45.0%
! TATTTGCTGCGCGTGGAATA+GGG + chr3.4:78168942-78168961 None:intergenic 45.0%
!! ACCAGAATCTGACTCGGATT+CGG - chr3.4:78168568-78168587 MS.gene71284:CDS 45.0%
!! ATCTGACTCGGATTCGGAAT+CGG - chr3.4:78168574-78168593 MS.gene71284:CDS 45.0%
ACCCGTTCTCCTATTCCTCT+TGG - chr3.4:78169192-78169211 MS.gene71284:CDS 50.0%
CCTCTGTTGTAGCAGCTTTC+CGG + chr3.4:78168518-78168537 None:intergenic 50.0%
CGGAAAGCTGCTACAACAGA+GGG - chr3.4:78168516-78168535 MS.gene71284:CDS 50.0%
CTCGGATTCGGAATCGGAAT+CGG - chr3.4:78168580-78168599 MS.gene71284:CDS 50.0%
CTCTGTTGTAGCAGCTTTCC+GGG + chr3.4:78168517-78168536 None:intergenic 50.0%
GGAATTGGACCCGTTATGGT+GGG - chr3.4:78168637-78168656 MS.gene71284:CDS 50.0%
GGTATCCGTTCCCACCATAA+CGG + chr3.4:78168650-78168669 None:intergenic 50.0%
GTATCCGTTCCCACCATAAC+GGG + chr3.4:78168649-78168668 None:intergenic 50.0%
TCCGAATCCGAGTCAGATTC+TGG + chr3.4:78168572-78168591 None:intergenic 50.0%
TCTGTTGTAGCAGCTTTCCG+GGG + chr3.4:78168516-78168535 None:intergenic 50.0%
TGGAATTGGACCCGTTATGG+TGG - chr3.4:78168636-78168655 MS.gene71284:CDS 50.0%
TGGGTTATGGGAAAGGGTCT+GGG + chr3.4:78168982-78169001 None:intergenic 50.0%
TTGGGTTATGGGAAAGGGTC+TGG + chr3.4:78168983-78169002 None:intergenic 50.0%
! ACGCCTCTCCAAACTTGCTT+TGG - chr3.4:78169023-78169042 MS.gene71284:CDS 50.0%
! AGTTGTTGTAGCAGGGGTGT+TGG + chr3.4:78169274-78169293 None:intergenic 50.0%
! GGAATAGGGATGGCATCGAA+GGG + chr3.4:78168928-78168947 None:intergenic 50.0%
! GTATTTGCTGCGCGTGGAAT+AGG + chr3.4:78168943-78168962 None:intergenic 50.0%
! TGGAATAGGGATGGCATCGA+AGG + chr3.4:78168929-78168948 None:intergenic 50.0%
!! CAACTTTTCAGGCCCATGTC+TGG - chr3.4:78169289-78169308 MS.gene71284:CDS 50.0%
!! TGGAGAGGCGTTTGAGAAGA+GGG + chr3.4:78169014-78169033 None:intergenic 50.0%
!! TTGGAGAGGCGTTTGAGAAG+AGG + chr3.4:78169015-78169034 None:intergenic 50.0%
ACAGAGGGAAGTGGCCGAAA+CGG - chr3.4:78168531-78168550 MS.gene71284:CDS 55.0%
CCACGCGCAGCAAATACATG+TGG - chr3.4:78168946-78168965 MS.gene71284:intron 55.0%
CCGGAAAGCTGCTACAACAG+AGG - chr3.4:78168515-78168534 MS.gene71284:CDS 55.0%
CTGTTGTAGCAGCTTTCCGG+GGG + chr3.4:78168515-78168534 None:intergenic 55.0%
GCTGCTACAACAGAGGGAAG+TGG - chr3.4:78168522-78168541 MS.gene71284:CDS 55.0%
GGCTCCAAACACGAGAGTCT+CGG + chr3.4:78168480-78168499 None:intergenic 55.0%
TCGACCGAGACTCTCGTGTT+TGG - chr3.4:78168473-78168492 MS.gene71284:CDS 55.0%
TCTCGTTCCGCTTCCCGTTT+CGG + chr3.4:78168548-78168567 None:intergenic 55.0%
! CCACATGTATTTGCTGCGCG+TGG + chr3.4:78168949-78168968 None:intergenic 55.0%
! TGGACCCGTTATGGTGGGAA+CGG - chr3.4:78168642-78168661 MS.gene71284:CDS 55.0%
!! AACGGGTTGCCTTGACAAGC+TGG + chr3.4:78169179-78169198 None:intergenic 55.0%
!! GTTGTAGCAGGGGTGTTGGA+AGG + chr3.4:78169270-78169289 None:intergenic 55.0%
CAGAGGGAAGTGGCCGAAAC+GGG - chr3.4:78168532-78168551 MS.gene71284:CDS 60.0%
GAAGTGGCCGAAACGGGAAG+CGG - chr3.4:78168538-78168557 MS.gene71284:CDS 60.0%
TGCTGCGCGTGGAATAGGGA+TGG + chr3.4:78168938-78168957 None:intergenic 60.0%
TTGTAGCAGCTTTCCGGGGG+AGG + chr3.4:78168512-78168531 None:intergenic 60.0%
AGCAGCTTTCCGGGGGAGGT+TGG + chr3.4:78168508-78168527 None:intergenic 65.0%
GCTTTCCGGGGGAGGTTGGA+TGG + chr3.4:78168504-78168523 None:intergenic 65.0%
TTCCGGGGGAGGTTGGATGG+CGG + chr3.4:78168501-78168520 None:intergenic 65.0%
AGCCGCCATCCAACCTCCCC+CGG - chr3.4:78168496-78168515 MS.gene71284:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 78168470 78169368 78168470 ID=MS.gene71284
chr3.4 mRNA 78168470 78169368 78168470 ID=MS.gene71284.t1;Parent=MS.gene71284
chr3.4 exon 78169000 78169368 78169000 ID=MS.gene71284.t1.exon1;Parent=MS.gene71284.t1
chr3.4 CDS 78169000 78169368 78169000 ID=cds.MS.gene71284.t1;Parent=MS.gene71284.t1
chr3.4 exon 78168770 78168913 78168770 ID=MS.gene71284.t1.exon2;Parent=MS.gene71284.t1
chr3.4 CDS 78168770 78168913 78168770 ID=cds.MS.gene71284.t1;Parent=MS.gene71284.t1
chr3.4 exon 78168470 78168679 78168470 ID=MS.gene71284.t1.exon3;Parent=MS.gene71284.t1
chr3.4 CDS 78168470 78168679 78168470 ID=cds.MS.gene71284.t1;Parent=MS.gene71284.t1
Gene Sequence

>MS.gene71284

ATGTCGACCGAGACTCTCGTGTTTGGAGCCGCCATCCAACCTCCCCCGGAAAGCTGCTACAACAGAGGGAAGTGGCCGAAACGGGAAGCGGAACGAGAACCAGAATCTGACTCGGATTCGGAATCGGAATCGGAATCAGAATCGGAAGTAGAATCGAAAGTGAAAGTGGAATTGGACCCGTTATGGTGGGAACGGATACCATTAGAAGATCTGAAGGAGAGTGATTTCGCTAGCCGTTATGAAGATGATCCTTTTCCTTACTTTTTTACGTGTCCGAAATTTGTTTATGAGAATAAAGCTATGAGAGATAAAGAAGAGGCTAGAAAAAATGCTGTCGAAAAGTATTGGAAGGAAGCTGAGAACATAAGTGTATGTTGTTTCCCTCTCTTAATTTTTGTTTTAATTTTGTATTTAACTCTTTTAATCTGTTTCTGTTTCTGTACTCTGCTGCACAGCCCTTCGATGCCATCCCTATTCCACGCGCAGCAAATACATGTGGCAATAACTATCCCAGACCCTTTCCCATAACCCAACAACGTATCCCTCTTCTCAAACGCCTCTCCAAACTTGCTTTGGAAAAATACAATGTTGACAATCAGGTTTTAAACTACTACTTCACCAATATTGTTTTTGTTTATATCTTATTCTTTAGTTCATCCGCTTCATTGTTCTTCCTTTCTCTTATTTAGGCCAAATATGAGTTTGTCCAGCTTGTCAAGGCAACCCGTTCTCCTATTCCTCTTGGTACTTATTACATTACTTTTAAGGCAAAACAACCAATACCTCAAGTTGTTGAACCTTCCAACACCCCTGCTACAACAACTTTTCAGGCCCATGTCTGGAACAAGATATCTGGAACAACTAAGGTCAAGTCGTGTTCCATTAAAACCTCTAATTAA

Protein sequence

>MS.gene71284.t1

MSTETLVFGAAIQPPPESCYNRGKWPKREAEREPESDSDSESESESESEVESKVKVELDPLWWERIPLEDLKESDFASRYEDDPFPYFFTCPKFVYENKAMRDKEEARKNAVEKYWKEAENISPFDAIPIPRAANTCGNNYPRPFPITQQRIPLLKRLSKLALEKYNVDNQAKYEFVQLVKATRSPIPLGTYYITFKAKQPIPQVVEPSNTPATTTFQAHVWNKISGTTKVKSCSIKTSN