AlfalfaGEDB | MS.gene044323

M. sativa cultivar XinJiangDaYe / MS.gene044323

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene044323.t1	PNX84641.1	52.3	172	76	5	23	190	78	247	3.60E-35	158.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene044323.t1	A0A2K3M1H4	52.3	172	76	5	23	190	78	247	2.6e-35	158.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene044211	MS.gene044323	PPI
MS.gene70433	MS.gene044323	PPI
MS.gene064006	MS.gene044323	PPI
MS.gene69757	MS.gene044323	PPI
MS.gene70443	MS.gene044323	PPI
MS.gene70949	MS.gene044323	PPI
MS.gene71282	MS.gene044323	PPI
MS.gene70425	MS.gene044323	PPI
MS.gene25756	MS.gene044323	PPI
MS.gene25749	MS.gene044323	PPI
MS.gene70424	MS.gene044323	PPI
MS.gene70427	MS.gene044323	PPI
MS.gene71528	MS.gene044323	PPI
MS.gene044376	MS.gene044323	PPI
MS.gene044363	MS.gene044323	PPI
MS.gene25703	MS.gene044323	PPI
MS.gene073908	MS.gene044323	PPI
MS.gene71524	MS.gene044323	PPI
MS.gene044369	MS.gene044323	PPI
MS.gene70430	MS.gene044323	PPI
MS.gene044364	MS.gene044323	PPI
MS.gene77464	MS.gene044323	PPI
MS.gene067686	MS.gene044323	PPI
MS.gene71529	MS.gene044323	PPI
MS.gene70418	MS.gene044323	PPI
MS.gene044282	MS.gene044323	PPI
MS.gene06352	MS.gene044323	PPI
MS.gene73915	MS.gene044323	PPI
MS.gene044375	MS.gene044323	PPI
MS.gene77469	MS.gene044323	PPI
MS.gene049263	MS.gene044323	PPI
MS.gene77468	MS.gene044323	PPI
MS.gene70434	MS.gene044323	PPI
MS.gene77462	MS.gene044323	PPI
MS.gene71284	MS.gene044323	PPI
MS.gene044323	MS.gene71524	PPI
MS.gene044326	MS.gene044323	PPI
MS.gene71285	MS.gene044323	PPI
MS.gene71286	MS.gene044323	PPI
MS.gene70426	MS.gene044323	PPI
MS.gene77472	MS.gene044323	PPI
MS.gene70431	MS.gene044323	PPI
MS.gene044215	MS.gene044323	PPI
MS.gene044359	MS.gene044323	PPI
MS.gene70511	MS.gene044323	PPI
MS.gene71531	MS.gene044323	PPI
MS.gene77461	MS.gene044323	PPI
MS.gene77466	MS.gene044323	PPI
MS.gene70441	MS.gene044323	PPI
MS.gene70476	MS.gene044323	PPI
MS.gene70436	MS.gene044323	PPI
MS.gene044323	MS.gene044326	PPI
MS.gene25704	MS.gene044323	PPI
MS.gene044377	MS.gene044323	PPI
MS.gene77467	MS.gene044323	PPI
MS.gene70428	MS.gene044323	PPI
MS.gene71530	MS.gene044323	PPI
MS.gene70445	MS.gene044323	PPI
MS.gene044243	MS.gene044323	PPI
MS.gene044284	MS.gene044323	PPI
MS.gene70439	MS.gene044323	PPI
MS.gene045214	MS.gene044323	PPI
MS.gene71527	MS.gene044323	PPI
MS.gene71532	MS.gene044323	PPI
MS.gene044361	MS.gene044323	PPI
MS.gene70440	MS.gene044323	PPI
MS.gene69758	MS.gene044323	PPI
MS.gene79115	MS.gene044323	PPI
MS.gene044370	MS.gene044323	PPI
MS.gene70516	MS.gene044323	PPI
MS.gene044323	MS.gene049263	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene044323.t1	MTR_3g083150	42.654	211	98	5	1	191	5	212	3.52e-40	136
MS.gene044323.t1	MTR_3g083080	46.429	168	82	6	23	190	46	205	1.31e-34	121
MS.gene044323.t1	MTR_3g083100	51.145	131	55	3	70	191	44	174	9.94e-30	108
MS.gene044323.t1	MTR_3g083050	33.178	214	111	6	1	192	5	208	1.14e-25	98.6
MS.gene044323.t1	MTR_3g083030	36.628	172	98	5	22	192	70	231	1.02e-24	96.7
MS.gene044323.t1	MTR_3g082420	36.757	185	102	8	12	190	46	221	6.86e-20	84.0
MS.gene044323.t1	MTR_3g082340	37.423	163	88	7	34	190	68	222	1.43e-18	80.5
MS.gene044323.t1	MTR_3g080680	31.481	162	99	6	33	190	45	198	8.28e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 54 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCGGTAAGGTCCATGGGTT+TGG	0.280360	3.4:+77779480	None:intergenic
CAAAGCTCGGACACATATAA+TGG	0.365368	3.4:+77779644	None:intergenic
ACATTATTACCGTTAATTAG+AGG	0.371942	3.4:+77779115	None:intergenic
CAAGTCCAGCACAAAATATC+AGG	0.381378	3.4:-77779170	MS.gene044323:CDS
ACGCCTCTCGCAGAGTGCTT+TGG	0.390531	3.4:-77779431	MS.gene044323:CDS
CACATATAATGGAAAGGATA+AGG	0.394916	3.4:+77779655	None:intergenic
CCATTAAAACCTCTAATTAA+CGG	0.395699	3.4:-77779124	None:intergenic
CGCGGCTTCCCGACGGAGGT+TGG	0.395709	3.4:+77779875	None:intergenic
TCGGTAAGGTCCATGGGTTT+GGG	0.408794	3.4:+77779481	None:intergenic
GGTTGGATGGCGCCAAACAC+GGG	0.417929	3.4:+77779892	None:intergenic
AGAGACGGTGGCATCATTAT+CGG	0.428484	3.4:+77779462	None:intergenic
ATCATTATCGGTAAGGTCCA+TGG	0.450685	3.4:+77779474	None:intergenic
TCAACGACAGAAAACGAAAC+AGG	0.463478	3.4:-77779596	MS.gene044323:CDS
TGTCGTTGATTCTTCATAGC+AGG	0.464152	3.4:+77779610	None:intergenic
AGGTTGGATGGCGCCAAACA+CGG	0.468013	3.4:+77779891	None:intergenic
ATGAACATGGTTGGCTTGAA+AGG	0.479169	3.4:+77779232	None:intergenic
GATAGCAGCAGAAGATCTGA+AGG	0.502982	3.4:-77779710	MS.gene044323:CDS
TGGGCTTGAAAAGTTGTAGC+GGG	0.505274	3.4:+77779191	None:intergenic
ATAAGCTTGTCAAGGCAACT+AGG	0.517046	3.4:-77779286	MS.gene044323:CDS
GCTTCCCGACGGAGGTTGGA+TGG	0.517222	3.4:+77779879	None:intergenic
GGCGCCATCCAACCTCCGTC+GGG	0.520169	3.4:-77779883	MS.gene044323:CDS
GTTGTAGCGGGGCAGTTGGA+TGG	0.522207	3.4:+77779203	None:intergenic
AATAAGTAACAAGAGGAATA+CGG	0.522696	3.4:+77779261	None:intergenic
AAAAGTTGTAGCGGGGCAGT+TGG	0.527773	3.4:+77779199	None:intergenic
ACATATAATGGAAAGGATAA+GGG	0.536682	3.4:+77779656	None:intergenic
TGGCGCCATCCAACCTCCGT+CGG	0.537484	3.4:-77779884	MS.gene044323:CDS
CGGTGGCATCATTATCGGTA+AGG	0.537827	3.4:+77779467	None:intergenic
CCACATACATTTGCATCTCG+TGG	0.539812	3.4:+77779514	None:intergenic
TCATTATCGGTAAGGTCCAT+GGG	0.554089	3.4:+77779475	None:intergenic
TGGAACATCAATAGCATCGA+AGG	0.565628	3.4:+77779534	None:intergenic
GATAAGGGTCTTTATCATAA+CGG	0.569387	3.4:+77779671	None:intergenic
GTTGGATGGCGCCAAACACG+GGG	0.576427	3.4:+77779893	None:intergenic
GGATTCATGTACACAAAGCT+CGG	0.583206	3.4:+77779631	None:intergenic
CTCGGACACATATAATGGAA+AGG	0.589760	3.4:+77779649	None:intergenic
ACAGAAAACGAAACAGGCCG+TGG	0.593963	3.4:-77779590	MS.gene044323:CDS
TTATCGTCGCGGCTTCCCGA+CGG	0.595175	3.4:+77779868	None:intergenic
CAAGTACAGTAGCGACAACC+AGG	0.598517	3.4:-77779407	MS.gene044323:intron
TGAGTTTCATAAGCTTGTCA+AGG	0.600500	3.4:-77779294	MS.gene044323:CDS
CAATAACTTTCCCAAACCCA+TGG	0.607239	3.4:-77779491	MS.gene044323:CDS
CCACGAGATGCAAATGTATG+TGG	0.613870	3.4:-77779514	MS.gene044323:CDS
TCGTCGCGGCTTCCCGACGG+AGG	0.615404	3.4:+77779871	None:intergenic
GTAATGTAATAAGTAACAAG+AGG	0.626696	3.4:+77779254	None:intergenic
TGGATCGACATGAACATGGT+TGG	0.632198	3.4:+77779223	None:intergenic
GCGTTTGAGAAGAGAGACGG+TGG	0.640198	3.4:+77779450	None:intergenic
TGTCCAAAGCACTCTGCGAG+AGG	0.641035	3.4:+77779428	None:intergenic
CCAGCACAAAATATCAGGAG+AGG	0.648052	3.4:-77779165	MS.gene044323:CDS
GAGGCGTTTGAGAAGAGAGA+CGG	0.648879	3.4:+77779447	None:intergenic
GGAACATCAATAGCATCGAA+GGG	0.657650	3.4:+77779535	None:intergenic
GATCTTTAAGTAATCAGCCA+CGG	0.658624	3.4:+77779573	None:intergenic
TTGGGCTTGAAAAGTTGTAG+CGG	0.659654	3.4:+77779190	None:intergenic
GGGCTTGAAAAGTTGTAGCG+GGG	0.663765	3.4:+77779192	None:intergenic
TGGATGGATCGACATGAACA+TGG	0.675076	3.4:+77779219	None:intergenic
GTGACTTTACTTTATCGTCG+CGG	0.703285	3.4:+77779857	None:intergenic
TTGGATGGCGCCAAACACGG+GGG	0.727941	3.4:+77779894	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAAGAATAAAATATAAGAA+TGG	+	chr3.4:77779665-77779684	None:intergenic	15.0%
!!	TTTCTCTTCTTATTAATTTA+GGG	-	chr3.4:77779702-77779721	MS.gene044323:CDS	15.0%
!!	AGAATAAAATATAAGAATGG+TGG	+	chr3.4:77779662-77779681	None:intergenic	20.0%
!!	CTTTCTCTTCTTATTAATTT+AGG	-	chr3.4:77779701-77779720	MS.gene044323:CDS	20.0%
!	AATAAGTAACAAGAGGAATA+CGG	+	chr3.4:77779770-77779789	None:intergenic	25.0%
!	GTAATGTAATAAGTAACAAG+AGG	+	chr3.4:77779777-77779796	None:intergenic	25.0%
!!	ACATATAATGGAAAGGATAA+GGG	+	chr3.4:77779375-77779394	None:intergenic	25.0%
	GATAAGGGTCTTTATCATAA+CGG	+	chr3.4:77779360-77779379	None:intergenic	30.0%
	TTCAGAATCAAAAGTGGAAT+CGG	-	chr3.4:77779276-77779295	MS.gene044323:CDS	30.0%
!	CACATATAATGGAAAGGATA+AGG	+	chr3.4:77779376-77779395	None:intergenic	30.0%
!	GCTATCTTTTCAAACCAAAA+CGG	+	chr3.4:77779304-77779323	None:intergenic	30.0%
	GATCTTTAAGTAATCAGCCA+CGG	+	chr3.4:77779458-77779477	None:intergenic	35.0%
	TGAGTTTCATAAGCTTGTCA+AGG	-	chr3.4:77779734-77779753	MS.gene044323:intron	35.0%
!	TGATATTTTGTGCTGGACTT+GGG	+	chr3.4:77779859-77779878	None:intergenic	35.0%
	ATAAGCTTGTCAAGGCAACT+AGG	-	chr3.4:77779742-77779761	MS.gene044323:intron	40.0%
	ATCATTATCGGTAAGGTCCA+TGG	+	chr3.4:77779557-77779576	None:intergenic	40.0%
	CAAAGCTCGGACACATATAA+TGG	+	chr3.4:77779387-77779406	None:intergenic	40.0%
	CAAGTCCAGCACAAAATATC+AGG	-	chr3.4:77779858-77779877	MS.gene044323:intron	40.0%
	CAATAACTTTCCCAAACCCA+TGG	-	chr3.4:77779537-77779556	MS.gene044323:CDS	40.0%
	GCGACGATAAAGTAAAGTCA+CGG	-	chr3.4:77779173-77779192	MS.gene044323:CDS	40.0%
	GGATTCATGTACACAAAGCT+CGG	+	chr3.4:77779400-77779419	None:intergenic	40.0%
	GTCGGATTCAGAATCAAAAG+TGG	-	chr3.4:77779270-77779289	MS.gene044323:CDS	40.0%
	GTGACTTTACTTTATCGTCG+CGG	+	chr3.4:77779174-77779193	None:intergenic	40.0%
	TCAACGACAGAAAACGAAAC+AGG	-	chr3.4:77779432-77779451	MS.gene044323:CDS	40.0%
	TCATTATCGGTAAGGTCCAT+GGG	+	chr3.4:77779556-77779575	None:intergenic	40.0%
	TTGGGCTTGAAAAGTTGTAG+CGG	+	chr3.4:77779841-77779860	None:intergenic	40.0%
!	CTCGGACACATATAATGGAA+AGG	+	chr3.4:77779382-77779401	None:intergenic	40.0%
!	CTGATATTTTGTGCTGGACT+TGG	+	chr3.4:77779860-77779879	None:intergenic	40.0%
!	GGAACATCAATAGCATCGAA+GGG	+	chr3.4:77779496-77779515	None:intergenic	40.0%
!	TGGAACATCAATAGCATCGA+AGG	+	chr3.4:77779497-77779516	None:intergenic	40.0%
!	TGGTGAAGTAGTTTAGAACC+TGG	+	chr3.4:77779642-77779661	None:intergenic	40.0%
!	TTCTGATTCCGGTTCTGATT+CGG	+	chr3.4:77779209-77779228	None:intergenic	40.0%
!!	ATCAGAATCTGACTCGGATT+CGG	-	chr3.4:77779222-77779241	MS.gene044323:CDS	40.0%
!!	ATGAACATGGTTGGCTTGAA+AGG	+	chr3.4:77779799-77779818	None:intergenic	40.0%
!!	TGTCGTTGATTCTTCATAGC+AGG	+	chr3.4:77779421-77779440	None:intergenic	40.0%
	ACCGGAATCAGAATCTGACT+CGG	-	chr3.4:77779216-77779235	MS.gene044323:CDS	45.0%
	AGAGACGGTGGCATCATTAT+CGG	+	chr3.4:77779569-77779588	None:intergenic	45.0%
	CCACATACATTTGCATCTCG+TGG	+	chr3.4:77779517-77779536	None:intergenic	45.0%
	CCACGAGATGCAAATGTATG+TGG	-	chr3.4:77779514-77779533	MS.gene044323:CDS	45.0%
	CCAGCACAAAATATCAGGAG+AGG	-	chr3.4:77779863-77779882	MS.gene044323:intron	45.0%
	GATAGCAGCAGAAGATCTGA+AGG	-	chr3.4:77779318-77779337	MS.gene044323:intron	45.0%
	TCCGACTCTGCTATCAATTC+CGG	+	chr3.4:77779256-77779275	None:intergenic	45.0%
	TCCGAGTCAGATTCTGATTC+CGG	+	chr3.4:77779220-77779239	None:intergenic	45.0%
	TGGATCGACATGAACATGGT+TGG	+	chr3.4:77779808-77779827	None:intergenic	45.0%
	TGGATGGATCGACATGAACA+TGG	+	chr3.4:77779812-77779831	None:intergenic	45.0%
	TGGGCTTGAAAAGTTGTAGC+GGG	+	chr3.4:77779840-77779859	None:intergenic	45.0%
!	AAGTGGAATCGGATCCGTTT+TGG	-	chr3.4:77779287-77779306	MS.gene044323:CDS	45.0%
!	ACCGGAATTGATAGCAGAGT+CGG	-	chr3.4:77779252-77779271	MS.gene044323:CDS	45.0%
!	CCTCTCCTGATATTTTGTGC+TGG	+	chr3.4:77779866-77779885	None:intergenic	45.0%
	ACAGAAAACGAAACAGGCCG+TGG	-	chr3.4:77779438-77779457	MS.gene044323:CDS	50.0%
	ACGTGAAGCCGAATCAGAAC+CGG	-	chr3.4:77779198-77779217	MS.gene044323:CDS	50.0%
	ATCGGTAAGGTCCATGGGTT+TGG	+	chr3.4:77779551-77779570	None:intergenic	50.0%
	CAAGTACAGTAGCGACAACC+AGG	-	chr3.4:77779621-77779640	MS.gene044323:CDS	50.0%
	CGGTGGCATCATTATCGGTA+AGG	+	chr3.4:77779564-77779583	None:intergenic	50.0%
	CTCGGATTCGGAATCTGAAC+CGG	-	chr3.4:77779234-77779253	MS.gene044323:CDS	50.0%
	GGGCTTGAAAAGTTGTAGCG+GGG	+	chr3.4:77779839-77779858	None:intergenic	50.0%
	TCGGTAAGGTCCATGGGTTT+GGG	+	chr3.4:77779550-77779569	None:intergenic	50.0%
!	AAAAGTTGTAGCGGGGCAGT+TGG	+	chr3.4:77779832-77779851	None:intergenic	50.0%
!	GAGGCGTTTGAGAAGAGAGA+CGG	+	chr3.4:77779584-77779603	None:intergenic	50.0%
	AGGTTGGATGGCGCCAAACA+CGG	+	chr3.4:77779140-77779159	None:intergenic	55.0%
	GCGTTTGAGAAGAGAGACGG+TGG	+	chr3.4:77779581-77779600	None:intergenic	55.0%
!	TGTCCAAAGCACTCTGCGAG+AGG	+	chr3.4:77779603-77779622	None:intergenic	55.0%
	GGTTGGATGGCGCCAAACAC+GGG	+	chr3.4:77779139-77779158	None:intergenic	60.0%
	GTTGGATGGCGCCAAACACG+GGG	+	chr3.4:77779138-77779157	None:intergenic	60.0%
	TTATCGTCGCGGCTTCCCGA+CGG	+	chr3.4:77779163-77779182	None:intergenic	60.0%
	TTGGATGGCGCCAAACACGG+GGG	+	chr3.4:77779137-77779156	None:intergenic	60.0%
!	ACGCCTCTCGCAGAGTGCTT+TGG	-	chr3.4:77779597-77779616	MS.gene044323:CDS	60.0%
!	GTTGTAGCGGGGCAGTTGGA+TGG	+	chr3.4:77779828-77779847	None:intergenic	60.0%
	GCTTCCCGACGGAGGTTGGA+TGG	+	chr3.4:77779152-77779171	None:intergenic	65.0%
	TGGCGCCATCCAACCTCCGT+CGG	-	chr3.4:77779144-77779163	MS.gene044323:CDS	65.0%
	GGCGCCATCCAACCTCCGTC+GGG	-	chr3.4:77779145-77779164	MS.gene044323:CDS	70.0%
	CGCGGCTTCCCGACGGAGGT+TGG	+	chr3.4:77779156-77779175	None:intergenic	75.0%
	TCGTCGCGGCTTCCCGACGG+AGG	+	chr3.4:77779160-77779179	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	77779127	77779923	77779127	ID=MS.gene044323
chr3.4	mRNA	77779127	77779923	77779127	ID=MS.gene044323.t1;Parent=MS.gene044323
chr3.4	exon	77779867	77779923	77779867	ID=MS.gene044323.t1.exon1;Parent=MS.gene044323.t1
chr3.4	CDS	77779867	77779923	77779867	ID=cds.MS.gene044323.t1;Parent=MS.gene044323.t1
chr3.4	exon	77779408	77779731	77779408	ID=MS.gene044323.t1.exon2;Parent=MS.gene044323.t1
chr3.4	CDS	77779408	77779731	77779408	ID=cds.MS.gene044323.t1;Parent=MS.gene044323.t1
chr3.4	exon	77779127	77779327	77779127	ID=MS.gene044323.t1.exon3;Parent=MS.gene044323.t1
chr3.4	CDS	77779127	77779327	77779127	ID=cds.MS.gene044323.t1;Parent=MS.gene044323.t1

Gene Sequence

>MS.gene044323

ATGGATACCCCCGTGTTTGGCGCCATCCAACCTCCGTCGGGAAGCCGCGACGATAAAGTAAAGTCACGGAAACGTGAAGCCGAATCAGAACCGGAATCAGAATCTGACTCGGATTCGGAATCTGAACCGGAATTGATAGCAGAGTCGGATTCAGAATCAAAAGTGGAATCGGATCCGTTTTGGTTTGAAAAGATAGCAGCAGAAGATCTGAAGGAGAGTGATTTCGCTGACCGTTATGATAAAGACCCTTATCCTTTCCATTATATGTGTCCGAGCTTTGTGTACATGAATCCTGCTATGAAGAATCAACGACAGAAAACGAAACAGGCCGTGGCTGATTACTTAAAGATCACTGAAGACATAAGTCCCTTCGATGCTATTGATGTTCCACGAGATGCAAATGTATGTGGCAATAACTTTCCCAAACCCATGGACCTTACCGATAATGATGCCACCGTCTCTCTTCTCAAACGCCTCTCGCAGAGTGCTTTGGACAAGTACAGTAGCGACAACCAGGTTCTAAACTACTTCACCACCATTCTTATATTTTATTCTTTCATTCATTATTCTTATTCTTTCTCTTCTTATTAATTTAGGGTATCAAGTATGAGTTTCATAAGCTTGTCAAGGCAACTAGGTGCCGTATTCCTCTTGTTACTTATTACATTACCTTTCAAGCCAACCATGTTCATGTCGATCCATCCAACTGCCCCGCTACAACTTTTCAAGCCCAAGTCCAGCACAAAATATCAGGAGAGGCTCTTGTCAAATCATGTTCCATTAAAACCTCTAATTAA

Protein sequence

>MS.gene044323.t1

MDTPVFGAIQPPSGSRDDKIAAEDLKESDFADRYDKDPYPFHYMCPSFVYMNPAMKNQRQKTKQAVADYLKITEDISPFDAIDVPRDANVCGNNFPKPMDLTDNDATVSLLKRLSQSALDKYSSDNQGIKYEFHKLVKATRCRIPLVTYYITFQANHVHVDPSNCPATTFQAQVQHKISGEALVKSCSIKTSN