AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70443


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70443.t1 MTR_3g083150 45.693 267 85 10 1 264 3 212 5.18e-48 159
MS.gene70443.t1 MTR_3g083030 34.572 269 130 12 2 265 4 231 4.02e-31 115
MS.gene70443.t1 MTR_3g083100 53.968 126 44 5 150 264 52 174 1.48e-30 112
MS.gene70443.t1 MTR_3g083050 33.333 270 113 12 1 265 1 208 2.65e-27 105
MS.gene70443.t1 MTR_3g082340 32.963 270 126 11 1 263 1 222 5.88e-23 94.0
MS.gene70443.t1 MTR_3g083080 37.129 202 90 7 63 263 40 205 1.21e-22 92.8
MS.gene70443.t1 MTR_3g082420 33.603 247 117 12 24 263 15 221 3.45e-20 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTGTTGTAGCAGCTTTC+CGG 0.155757 3.1:-66388203 None:intergenic
TTGGGTTATGGGAAAGGGTC+TGG 0.210252 3.1:-66388660 None:intergenic
AGGGATACGTTGTTGGGTTA+TGG 0.235089 3.1:-66388672 None:intergenic
TCCGAATCCGAGTCAGATTC+TGG 0.250389 3.1:-66388257 None:intergenic
CTCTGTTGTAGCAGCTTTCC+GGG 0.261650 3.1:-66388202 None:intergenic
GGGATACGTTGTTGGGTTAT+GGG 0.293781 3.1:-66388671 None:intergenic
TTATTCTCATAAACAAATTT+CGG 0.296115 3.1:-66388431 None:intergenic
AAGCTGGACAAACTCATATT+TGG 0.298094 3.1:-66388846 None:intergenic
TCTCGTTCCGCTTCCCGTTT+CGG 0.333023 3.1:-66388233 None:intergenic
GTATTTGCTGCGCGTGGAAT+CGG 0.335426 3.1:-66388620 None:intergenic
TACCATGGTGCGTTAATTAG+AGG 0.349376 3.1:-66389044 None:intergenic
ACGCCTCTCCAAACTTGCTT+TGG 0.354815 3.1:+66388703 MS.gene70443:CDS
TGGAGAGGCGTTTGAGAAGA+GGG 0.361494 3.1:-66388691 None:intergenic
GGTTCAACAACTTGAGGTAT+TGG 0.363055 3.1:-66388932 None:intergenic
AAAATACAATGTTGACAATC+AGG 0.364241 3.1:+66388727 MS.gene70443:CDS
ATCGAAAGTGAAAGTGGAAT+TGG 0.367090 3.1:+66388310 MS.gene70443:CDS
TCGACCGAGACTCTCGTGTT+TGG 0.383497 3.1:+66388161 MS.gene70443:CDS
TGGGTTATGGGAAAGGGTCT+GGG 0.394715 3.1:-66388659 None:intergenic
GAGAAGAGGGATACGTTGTT+GGG 0.411423 3.1:-66388678 None:intergenic
TAATAAGTACCAAGAGGAAT+AGG 0.423275 3.1:-66388887 None:intergenic
CTCGGATTCGGAATCGGAAT+CGG 0.425215 3.1:+66388268 MS.gene70443:CDS
ATCTGACTCGGATTCGGAAT+CGG 0.428389 3.1:+66388262 MS.gene70443:CDS
ACCAGAATCTGACTCGGATT+CGG 0.435178 3.1:+66388256 MS.gene70443:CDS
CAGAGGGAAGTGGCCGAAAC+GGG 0.441096 3.1:+66388220 MS.gene70443:CDS
TGAGAAGAGGGATACGTTGT+TGG 0.443257 3.1:-66388679 None:intergenic
CTCTCCTTCAGATCTTCTAA+TGG 0.445268 3.1:-66388356 None:intergenic
TATTTGCTGCGCGTGGAATC+GGG 0.448022 3.1:-66388619 None:intergenic
GCCTGAAAAGTTGTTGTAGC+AGG 0.449700 3.1:-66388965 None:intergenic
ACCCGTTCTCCTATTCCTCT+TGG 0.450300 3.1:+66388878 MS.gene70443:CDS
CATGTCTGGAACAAGATATC+TGG 0.451078 3.1:+66388989 MS.gene70443:CDS
AGTTGTTGTAGCAGGGGTGT+TGG 0.460802 3.1:-66388957 None:intergenic
TTGGAGAGGCGTTTGAGAAG+AGG 0.471987 3.1:-66388692 None:intergenic
AACGGGTTGCCTTGACAAGC+TGG 0.476282 3.1:-66388862 None:intergenic
AAGTGGAATTGGACCCGTTA+TGG 0.482799 3.1:+66388321 MS.gene70443:CDS
AAAATGCTGTCGAAAAGTAT+TGG 0.492089 3.1:+66388483 MS.gene70443:CDS
GTATCCGTTCCCACCATAAC+GGG 0.494522 3.1:-66388334 None:intergenic
ACCAAGAGGAATAGGAGAAC+GGG 0.497967 3.1:-66388879 None:intergenic
CAAGATATCTGGAACAACTA+AGG 0.499344 3.1:+66389000 MS.gene70443:CDS
CAGATATCTTGTTCCAGACA+TGG 0.500861 3.1:-66388988 None:intergenic
ACGTTGTTGGGTTATGGGAA+AGG 0.502825 3.1:-66388666 None:intergenic
AGCCGCCATCCAACCTCCCC+CGG 0.506131 3.1:+66388184 MS.gene70443:CDS
ACAGAGGGAAGTGGCCGAAA+CGG 0.508391 3.1:+66388219 MS.gene70443:CDS
ATCGGAATCGGAATCAGAAT+CGG 0.511451 3.1:+66388280 MS.gene70443:CDS
GTTGTAGCAGGGGTGTTGGA+AGG 0.514159 3.1:-66388953 None:intergenic
CGTTGTTGGGTTATGGGAAA+GGG 0.515504 3.1:-66388665 None:intergenic
GAAGTGGCCGAAACGGGAAG+CGG 0.529767 3.1:+66388226 MS.gene70443:CDS
GGTATCCGTTCCCACCATAA+CGG 0.532487 3.1:-66388335 None:intergenic
TGGAATCGGGATGGCATCGA+AGG 0.539991 3.1:-66388606 None:intergenic
AGTAGAATCGAAAGTGAAAG+TGG 0.543261 3.1:+66388304 MS.gene70443:CDS
AGATATCTTGTTCCAGACAT+GGG 0.545976 3.1:-66388987 None:intergenic
GAAAAGGATCATCTTCATAA+CGG 0.546737 3.1:-66388391 None:intergenic
GGCTCCAAACACGAGAGTCT+CGG 0.559644 3.1:-66388165 None:intergenic
TGGAATTGGACCCGTTATGG+TGG 0.566427 3.1:+66388324 MS.gene70443:CDS
TCTGTTGTAGCAGCTTTCCG+GGG 0.568607 3.1:-66388201 None:intergenic
TACCAAGAGGAATAGGAGAA+CGG 0.570966 3.1:-66388880 None:intergenic
TGCTGTCGAAAAGTATTGGA+AGG 0.572056 3.1:+66388487 MS.gene70443:CDS
GATACCATTAGAAGATCTGA+AGG 0.573494 3.1:+66388352 MS.gene70443:CDS
TTGGAAGGTTCAACAACTTG+AGG 0.575752 3.1:-66388938 None:intergenic
TGGACCCGTTATGGTGGGAA+CGG 0.578043 3.1:+66388330 MS.gene70443:CDS
CGGAAAGCTGCTACAACAGA+GGG 0.584202 3.1:+66388204 MS.gene70443:CDS
TGAGTTTGTCCAGCTTGTCA+AGG 0.586521 3.1:+66388853 MS.gene70443:CDS
CCGGAAAGCTGCTACAACAG+AGG 0.589302 3.1:+66388203 MS.gene70443:CDS
CCTGAAAAGTTGTTGTAGCA+GGG 0.590814 3.1:-66388964 None:intergenic
CTGAAAAGTTGTTGTAGCAG+GGG 0.591211 3.1:-66388963 None:intergenic
GCTGCTACAACAGAGGGAAG+TGG 0.596216 3.1:+66388210 MS.gene70443:CDS
CCACATGTATTTGCTGCGCG+TGG 0.600455 3.1:-66388626 None:intergenic
TTTCCAAAGCAAGTTTGGAG+AGG 0.618640 3.1:-66388706 None:intergenic
GGAATCGGGATGGCATCGAA+GGG 0.620758 3.1:-66388605 None:intergenic
AGCTATGAGAGATAAAGAAG+AGG 0.620914 3.1:+66388454 MS.gene70443:CDS
TGCTGCGCGTGGAATCGGGA+TGG 0.624872 3.1:-66388615 None:intergenic
GGAATTGGACCCGTTATGGT+GGG 0.637797 3.1:+66388325 MS.gene70443:CDS
CTGTTGTAGCAGCTTTCCGG+GGG 0.645513 3.1:-66388200 None:intergenic
ACGAGAACCAGAATCTGACT+CGG 0.655126 3.1:+66388250 MS.gene70443:CDS
GTAATGTAATAAGTACCAAG+AGG 0.663480 3.1:-66388893 None:intergenic
CCACGCGCAGCAAATACATG+TGG 0.685685 3.1:+66388626 MS.gene70443:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAGATATAAACAA+TGG - chr3.1:66388780-66388799 None:intergenic 10.0%
!! ATTAAAACAAAAATTAAGAG+AGG - chr3.1:66388534-66388553 None:intergenic 15.0%
!! TTAAAACAAAAATTAAGAGA+GGG - chr3.1:66388533-66388552 None:intergenic 15.0%
!! TTATTCTCATAAACAAATTT+CGG - chr3.1:66388434-66388453 None:intergenic 15.0%
!!! TACTTATTACATTACTTTTA+AGG + chr3.1:66388901-66388920 MS.gene70443:CDS 15.0%
!! AAATAAGATATAAACAATGG+TGG - chr3.1:66388777-66388796 None:intergenic 20.0%
! AAAATACAATGTTGACAATC+AGG + chr3.1:66388727-66388746 MS.gene70443:CDS 25.0%
!! TCTTTCTGTTTCTCTTATTT+AGG + chr3.1:66388823-66388842 MS.gene70443:intron 25.0%
AGAAACAGAAAGAACAATGA+AGG - chr3.1:66388816-66388835 None:intergenic 30.0%
CACGTAAAAAAGTAAGGAAA+AGG - chr3.1:66388410-66388429 None:intergenic 30.0%
GAAAAGGATCATCTTCATAA+CGG - chr3.1:66388394-66388413 None:intergenic 30.0%
GTAATGTAATAAGTACCAAG+AGG - chr3.1:66388896-66388915 None:intergenic 30.0%
TAATAAGTACCAAGAGGAAT+AGG - chr3.1:66388890-66388909 None:intergenic 30.0%
! AAAATGCTGTCGAAAAGTAT+TGG + chr3.1:66388483-66388502 MS.gene70443:CDS 30.0%
! GTATTTTTCCAAAGCAAGTT+TGG - chr3.1:66388714-66388733 None:intergenic 30.0%
AAGCTGGACAAACTCATATT+TGG - chr3.1:66388849-66388868 None:intergenic 35.0%
AGATATCTTGTTCCAGACAT+GGG - chr3.1:66388990-66389009 None:intergenic 35.0%
AGCTATGAGAGATAAAGAAG+AGG + chr3.1:66388454-66388473 MS.gene70443:CDS 35.0%
AGTAGAATCGAAAGTGAAAG+TGG + chr3.1:66388304-66388323 MS.gene70443:CDS 35.0%
ATCGAAAGTGAAAGTGGAAT+TGG + chr3.1:66388310-66388329 MS.gene70443:CDS 35.0%
GATACCATTAGAAGATCTGA+AGG + chr3.1:66388352-66388371 MS.gene70443:CDS 35.0%
TTCGGACACGTAAAAAAGTA+AGG - chr3.1:66388416-66388435 None:intergenic 35.0%
! CAAGATATCTGGAACAACTA+AGG + chr3.1:66389000-66389019 MS.gene70443:CDS 35.0%
ATCGGAATCGGAATCAGAAT+CGG + chr3.1:66388280-66388299 MS.gene70443:CDS 40.0%
CAGATATCTTGTTCCAGACA+TGG - chr3.1:66388991-66389010 None:intergenic 40.0%
CCTGAAAAGTTGTTGTAGCA+GGG - chr3.1:66388967-66388986 None:intergenic 40.0%
CTCTCCTTCAGATCTTCTAA+TGG - chr3.1:66388359-66388378 None:intergenic 40.0%
CTGAAAAGTTGTTGTAGCAG+GGG - chr3.1:66388966-66388985 None:intergenic 40.0%
GGTTCAACAACTTGAGGTAT+TGG - chr3.1:66388935-66388954 None:intergenic 40.0%
TACCAAGAGGAATAGGAGAA+CGG - chr3.1:66388883-66388902 None:intergenic 40.0%
TTGGAAGGTTCAACAACTTG+AGG - chr3.1:66388941-66388960 None:intergenic 40.0%
TTTCCAAAGCAAGTTTGGAG+AGG - chr3.1:66388709-66388728 None:intergenic 40.0%
! CATGTCTGGAACAAGATATC+TGG + chr3.1:66388989-66389008 MS.gene70443:CDS 40.0%
!! TGCTGTCGAAAAGTATTGGA+AGG + chr3.1:66388487-66388506 MS.gene70443:CDS 40.0%
AAGTGGAATTGGACCCGTTA+TGG + chr3.1:66388321-66388340 MS.gene70443:CDS 45.0%
ACCAAGAGGAATAGGAGAAC+GGG - chr3.1:66388882-66388901 None:intergenic 45.0%
ACGAGAACCAGAATCTGACT+CGG + chr3.1:66388250-66388269 MS.gene70443:CDS 45.0%
ACGTTGTTGGGTTATGGGAA+AGG - chr3.1:66388669-66388688 None:intergenic 45.0%
AGGGATACGTTGTTGGGTTA+TGG - chr3.1:66388675-66388694 None:intergenic 45.0%
CGTTGTTGGGTTATGGGAAA+GGG - chr3.1:66388668-66388687 None:intergenic 45.0%
GAGAAGAGGGATACGTTGTT+GGG - chr3.1:66388681-66388700 None:intergenic 45.0%
GCCTGAAAAGTTGTTGTAGC+AGG - chr3.1:66388968-66388987 None:intergenic 45.0%
GGGATACGTTGTTGGGTTAT+GGG - chr3.1:66388674-66388693 None:intergenic 45.0%
TGAGAAGAGGGATACGTTGT+TGG - chr3.1:66388682-66388701 None:intergenic 45.0%
TGAGTTTGTCCAGCTTGTCA+AGG + chr3.1:66388853-66388872 MS.gene70443:CDS 45.0%
! CCCTGCTACAACAACTTTTC+AGG + chr3.1:66388964-66388983 MS.gene70443:CDS 45.0%
!! ACCAGAATCTGACTCGGATT+CGG + chr3.1:66388256-66388275 MS.gene70443:CDS 45.0%
!! ATCTGACTCGGATTCGGAAT+CGG + chr3.1:66388262-66388281 MS.gene70443:CDS 45.0%
ACCCGTTCTCCTATTCCTCT+TGG + chr3.1:66388878-66388897 MS.gene70443:CDS 50.0%
CCTCTGTTGTAGCAGCTTTC+CGG - chr3.1:66388206-66388225 None:intergenic 50.0%
CGGAAAGCTGCTACAACAGA+GGG + chr3.1:66388204-66388223 MS.gene70443:CDS 50.0%
CTCGGATTCGGAATCGGAAT+CGG + chr3.1:66388268-66388287 MS.gene70443:CDS 50.0%
CTCTGTTGTAGCAGCTTTCC+GGG - chr3.1:66388205-66388224 None:intergenic 50.0%
GGAATTGGACCCGTTATGGT+GGG + chr3.1:66388325-66388344 MS.gene70443:CDS 50.0%
GGTATCCGTTCCCACCATAA+CGG - chr3.1:66388338-66388357 None:intergenic 50.0%
GTATCCGTTCCCACCATAAC+GGG - chr3.1:66388337-66388356 None:intergenic 50.0%
TCCGAATCCGAGTCAGATTC+TGG - chr3.1:66388260-66388279 None:intergenic 50.0%
TCTGTTGTAGCAGCTTTCCG+GGG - chr3.1:66388204-66388223 None:intergenic 50.0%
TGGAATTGGACCCGTTATGG+TGG + chr3.1:66388324-66388343 MS.gene70443:CDS 50.0%
TGGGTTATGGGAAAGGGTCT+GGG - chr3.1:66388662-66388681 None:intergenic 50.0%
TTGGGTTATGGGAAAGGGTC+TGG - chr3.1:66388663-66388682 None:intergenic 50.0%
! ACGCCTCTCCAAACTTGCTT+TGG + chr3.1:66388703-66388722 MS.gene70443:CDS 50.0%
! AGTTGTTGTAGCAGGGGTGT+TGG - chr3.1:66388960-66388979 None:intergenic 50.0%
! GTATTTGCTGCGCGTGGAAT+CGG - chr3.1:66388623-66388642 None:intergenic 50.0%
! TATTTGCTGCGCGTGGAATC+GGG - chr3.1:66388622-66388641 None:intergenic 50.0%
!! CAACTTTTCAGGCCCATGTC+TGG + chr3.1:66388975-66388994 MS.gene70443:CDS 50.0%
!! TGGAGAGGCGTTTGAGAAGA+GGG - chr3.1:66388694-66388713 None:intergenic 50.0%
!! TTGGAGAGGCGTTTGAGAAG+AGG - chr3.1:66388695-66388714 None:intergenic 50.0%
ACAGAGGGAAGTGGCCGAAA+CGG + chr3.1:66388219-66388238 MS.gene70443:CDS 55.0%
CCACGCGCAGCAAATACATG+TGG + chr3.1:66388626-66388645 MS.gene70443:CDS 55.0%
CCGGAAAGCTGCTACAACAG+AGG + chr3.1:66388203-66388222 MS.gene70443:CDS 55.0%
CTGTTGTAGCAGCTTTCCGG+GGG - chr3.1:66388203-66388222 None:intergenic 55.0%
GCTGCTACAACAGAGGGAAG+TGG + chr3.1:66388210-66388229 MS.gene70443:CDS 55.0%
GGCTCCAAACACGAGAGTCT+CGG - chr3.1:66388168-66388187 None:intergenic 55.0%
TCGACCGAGACTCTCGTGTT+TGG + chr3.1:66388161-66388180 MS.gene70443:CDS 55.0%
TCTCGTTCCGCTTCCCGTTT+CGG - chr3.1:66388236-66388255 None:intergenic 55.0%
! CCACATGTATTTGCTGCGCG+TGG - chr3.1:66388629-66388648 None:intergenic 55.0%
! GGAATCGGGATGGCATCGAA+GGG - chr3.1:66388608-66388627 None:intergenic 55.0%
! TGGAATCGGGATGGCATCGA+AGG - chr3.1:66388609-66388628 None:intergenic 55.0%
! TGGACCCGTTATGGTGGGAA+CGG + chr3.1:66388330-66388349 MS.gene70443:CDS 55.0%
!! AACGGGTTGCCTTGACAAGC+TGG - chr3.1:66388865-66388884 None:intergenic 55.0%
!! GTTGTAGCAGGGGTGTTGGA+AGG - chr3.1:66388956-66388975 None:intergenic 55.0%
CAGAGGGAAGTGGCCGAAAC+GGG + chr3.1:66388220-66388239 MS.gene70443:CDS 60.0%
GAAGTGGCCGAAACGGGAAG+CGG + chr3.1:66388226-66388245 MS.gene70443:CDS 60.0%
TTGTAGCAGCTTTCCGGGGG+AGG - chr3.1:66388200-66388219 None:intergenic 60.0%
AGCAGCTTTCCGGGGGAGGT+TGG - chr3.1:66388196-66388215 None:intergenic 65.0%
GCTTTCCGGGGGAGGTTGGA+TGG - chr3.1:66388192-66388211 None:intergenic 65.0%
TGCTGCGCGTGGAATCGGGA+TGG - chr3.1:66388618-66388637 None:intergenic 65.0%
TTCCGGGGGAGGTTGGATGG+CGG - chr3.1:66388189-66388208 None:intergenic 65.0%
AGCCGCCATCCAACCTCCCC+CGG + chr3.1:66388184-66388203 MS.gene70443:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 66388158 66389054 66388158 ID=MS.gene70443
chr3.1 mRNA 66388158 66389054 66388158 ID=MS.gene70443.t1;Parent=MS.gene70443
chr3.1 exon 66388158 66388748 66388158 ID=MS.gene70443.t1.exon1;Parent=MS.gene70443.t1
chr3.1 CDS 66388158 66388748 66388158 ID=cds.MS.gene70443.t1;Parent=MS.gene70443.t1
chr3.1 exon 66388845 66389054 66388845 ID=MS.gene70443.t1.exon2;Parent=MS.gene70443.t1
chr3.1 CDS 66388845 66389054 66388845 ID=cds.MS.gene70443.t1;Parent=MS.gene70443.t1
Gene Sequence

>MS.gene70443

ATGTCGACCGAGACTCTCGTGTTTGGAGCCGCCATCCAACCTCCCCCGGAAAGCTGCTACAACAGAGGGAAGTGGCCGAAACGGGAAGCGGAACGAGAACCAGAATCTGACTCGGATTCGGAATCGGAATCGGAATCAGAATCGGAAGTAGAATCGAAAGTGAAAGTGGAATTGGACCCGTTATGGTGGGAACGGATACCATTAGAAGATCTGAAGGAGAGTGATTTCGCTAGCCGTTATGAAGATGATCCTTTTCCTTACTTTTTTACGTGTCCGAAATTTGTTTATGAGAATAAAGCTATGAGAGATAAAGAAGAGGCTAGAAAAAATGCTGTCGAAAAGTATTGGAAGGAAGCTGAGAACATGTTGTTTCCCTCTCTTAATTTTTGTTTTAATTTTGTATTTAACTCTTTTAATCTGTTTCTGTTTCTGTACTCTGCTGCACAGCCCTTCGATGCCATCCCGATTCCACGCGCAGCAAATACATGTGGCAATAACTATCCCAGACCCTTTCCCATAACCCAACAACGTATCCCTCTTCTCAAACGCCTCTCCAAACTTGCTTTGGAAAAATACAATGTTGACAATCAGGTTTTAAACTAACTACTTCAGTTCACCACCATTGTTTATATCTTATTTATTCTTTTGTTCATCACCTTCATTGTTCTTTCTGTTTCTCTTATTTAGGCCAAATATGAGTTTGTCCAGCTTGTCAAGGCAACCCGTTCTCCTATTCCTCTTGGTACTTATTACATTACTTTTAAGGCAAAACAACCAATACCTCAAGTTGTTGAACCTTCCAACACCCCTGCTACAACAACTTTTCAGGCCCATGTCTGGAACAAGATATCTGGAACAACTAAGGTCAAGTCGTGTTCCATTAAAACCTCTAATTAA

Protein sequence

>MS.gene70443.t1

MSTETLVFGAAIQPPPESCYNRGKWPKREAEREPESDSDSESESESESEVESKVKVELDPLWWERIPLEDLKESDFASRYEDDPFPYFFTCPKFVYENKAMRDKEEARKNAVEKYWKEAENMLFPSLNFCFNFVFNSFNLFLFLYSAAQPFDAIPIPRAANTCGNNYPRPFPITQQRIPLLKRLSKLALEKYNVDNQAKYEFVQLVKATRSPIPLGTYYITFKAKQPIPQVVEPSNTPATTTFQAHVWNKISGTTKVKSCSIKTSN