AlfalfaGEDB | MS.gene045214

M. sativa cultivar XinJiangDaYe / MS.gene045214

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00031.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045214.t1	AES72093.2	62.5	168	37	4	1	168	1	142	1.70E-42	182.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045214.t1	G7J2I6	62.5	168	37	4	1	168	1	142	1.2e-42	182.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene71532	MS.gene045214	PPI
MS.gene044361	MS.gene045214	PPI
MS.gene70440	MS.gene045214	PPI
MS.gene79115	MS.gene045214	PPI
MS.gene044370	MS.gene045214	PPI
MS.gene70516	MS.gene045214	PPI
MS.gene70476	MS.gene045214	PPI
MS.gene045214	MS.gene25749	PPI
MS.gene044377	MS.gene045214	PPI
MS.gene044211	MS.gene045214	PPI
MS.gene045214	MS.gene70439	PPI
MS.gene69757	MS.gene045214	PPI
MS.gene045214	MS.gene71524	PPI
MS.gene70443	MS.gene045214	PPI
MS.gene045214	MS.gene70424	PPI
MS.gene71282	MS.gene045214	PPI
MS.gene70425	MS.gene045214	PPI
MS.gene044284	MS.gene045214	PPI
MS.gene25756	MS.gene045214	PPI
MS.gene71528	MS.gene045214	PPI
MS.gene71527	MS.gene045214	PPI
MS.gene044363	MS.gene045214	PPI
MS.gene045214	MS.gene77464	PPI
MS.gene25703	MS.gene045214	PPI
MS.gene69758	MS.gene045214	PPI
MS.gene70430	MS.gene045214	PPI
MS.gene044364	MS.gene045214	PPI
MS.gene045214	MS.gene044359	PPI
MS.gene045214	MS.gene049263	PPI
MS.gene045214	MS.gene044326	PPI
MS.gene70433	MS.gene045214	PPI
MS.gene064006	MS.gene045214	PPI
MS.gene71529	MS.gene045214	PPI
MS.gene044282	MS.gene045214	PPI
MS.gene70949	MS.gene045214	PPI
MS.gene045214	MS.gene70427	PPI
MS.gene06352	MS.gene045214	PPI
MS.gene73915	MS.gene045214	PPI
MS.gene044375	MS.gene045214	PPI
MS.gene045214	MS.gene71286	PPI
MS.gene045214	MS.gene044215	PPI
MS.gene044376	MS.gene045214	PPI
MS.gene77469	MS.gene045214	PPI
MS.gene073908	MS.gene045214	PPI
MS.gene044369	MS.gene045214	PPI
MS.gene77464	MS.gene045214	PPI
MS.gene71284	MS.gene045214	PPI
MS.gene71285	MS.gene045214	PPI
MS.gene70426	MS.gene045214	PPI
MS.gene77472	MS.gene045214	PPI
MS.gene70431	MS.gene045214	PPI
MS.gene70418	MS.gene045214	PPI
MS.gene70511	MS.gene045214	PPI
MS.gene045214	MS.gene70441	PPI
MS.gene77461	MS.gene045214	PPI
MS.gene77466	MS.gene045214	PPI
MS.gene045214	MS.gene71531	PPI
MS.gene045214	MS.gene77462	PPI
MS.gene045214	MS.gene044323	PPI
MS.gene70436	MS.gene045214	PPI
MS.gene77468	MS.gene045214	PPI
MS.gene25704	MS.gene045214	PPI
MS.gene70434	MS.gene045214	PPI
MS.gene77467	MS.gene045214	PPI
MS.gene045214	MS.gene71282	PPI
MS.gene70428	MS.gene045214	PPI
MS.gene71530	MS.gene045214	PPI
MS.gene70445	MS.gene045214	PPI
MS.gene045214	MS.gene71528	PPI
MS.gene044243	MS.gene045214	PPI
MS.gene045214	MS.gene067686	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045214.t1	MTR_3g086030	62.500	168	37	4	1	168	1	142	9.93e-59	181
MS.gene045214.t1	MTR_3g083080	46.860	207	88	5	1	187	1	205	1.11e-52	167
MS.gene045214.t1	MTR_3g083050	46.445	211	86	6	1	188	1	207	7.88e-52	165
MS.gene045214.t1	MTR_3g083030	50.811	185	76	5	15	188	50	230	8.38e-51	163
MS.gene045214.t1	MTR_3g083150	41.204	216	95	8	1	188	1	212	5.63e-30	109
MS.gene045214.t1	MTR_3g082420	42.012	169	85	7	28	187	57	221	5.73e-30	110
MS.gene045214.t1	MTR_3g080680	41.243	177	90	6	21	187	26	198	1.15e-29	108
MS.gene045214.t1	MTR_3g082340	41.566	166	84	7	31	187	61	222	6.94e-29	107
MS.gene045214.t1	MTR_3g083100	46.825	126	51	3	79	188	49	174	8.88e-25	95.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 44 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGACCTCCTCCGCTGAAAT+TGG	0.233701	3.1:+68873174	MS.gene045214:CDS
TGTCAAGTCAACTCGAAAAT+TGG	0.257704	3.1:+68873633	MS.gene045214:CDS
TTGCCTCTCCAAACTTGCTT+TGG	0.332332	3.1:+68873468	MS.gene045214:CDS
TTGCAGAATGTCGATGGAAT+TGG	0.342181	3.1:+68873129	None:intergenic
CAGACCTCTTGTTCCAGACT+TGG	0.349258	3.1:-68873738	None:intergenic
CCAGCTAAACCTGCTATTGC+AGG	0.361502	3.1:-68873400	None:intergenic
TCTTCAACAGGTCTCCATTT+CGG	0.369598	3.1:-68873199	None:intergenic
TGGGCCTGGAAAATTGTATC+AGG	0.383119	3.1:-68873718	None:intergenic
CGCTGAAATTGGAACCGAAA+TGG	0.389800	3.1:+68873185	MS.gene045214:CDS
GTAGTCGTCCAAAGCAAGTT+TGG	0.392482	3.1:-68873476	None:intergenic
TCAACTCGAAAATTGGTTAC+TGG	0.397529	3.1:+68873640	MS.gene045214:CDS
TCGCTTTGAGTATAAGAATA+AGG	0.404801	3.1:+68873285	MS.gene045214:CDS
TTCAACTCAACATCTTCAAC+AGG	0.405309	3.1:-68873211	None:intergenic
GAATTGGATTCCCGCTTGTT+CGG	0.412872	3.1:+68873145	MS.gene045214:CDS
CGACTACAATCATAAGAATC+AGG	0.442857	3.1:+68873492	MS.gene045214:CDS
GGTTCCAATTTCAGCGGAGG+AGG	0.445623	3.1:-68873178	None:intergenic
CCTGCAATAGCAGGTTTAGC+TGG	0.471208	3.1:+68873400	MS.gene045214:CDS
TCTTGTTCCAGACTTGGGCC+TGG	0.477668	3.1:-68873732	None:intergenic
AGACCTCTTGTTCCAGACTT+GGG	0.479027	3.1:-68873737	None:intergenic
CGATCACACTTAATCACAAA+AGG	0.481304	3.1:-68873265	None:intergenic
ATGGCACACGACTTGACCTC+AGG	0.506486	3.1:-68873763	None:intergenic
ATCGCCCCTCCTGCAATAGC+AGG	0.508072	3.1:+68873391	MS.gene045214:CDS
CGATAGCATCAAAGGGACTT+AGG	0.513793	3.1:-68873372	None:intergenic
CTGGAAAATTGTATCAGGAT+TGG	0.515830	3.1:-68873713	None:intergenic
GAACAAGAGGTCTGCACCTG+AGG	0.544263	3.1:+68873747	MS.gene045214:CDS
AGGAGGGGCGATAGCATCAA+AGG	0.546475	3.1:-68873380	None:intergenic
TTGCAATTGCAGAATGTCGA+TGG	0.566979	3.1:+68873123	None:intergenic
GCGCTTGTCGTACTCCGCCA+TGG	0.567075	3.1:-68873341	None:intergenic
CATTTCGGTTCCAATTTCAG+CGG	0.570500	3.1:-68873184	None:intergenic
GTCGAGTAACGCCGAACAAG+CGG	0.580577	3.1:-68873156	None:intergenic
ACTCGAAAATTGGTTACTGG+TGG	0.583640	3.1:+68873643	MS.gene045214:CDS
TTCGGTTCCAATTTCAGCGG+AGG	0.584740	3.1:-68873181	None:intergenic
TAAACCTGCTATTGCAGGAG+GGG	0.586552	3.1:-68873395	None:intergenic
TCGAGTAACGCCGAACAAGC+GGG	0.589918	3.1:-68873155	None:intergenic
GCTAAACCTGCTATTGCAGG+AGG	0.591208	3.1:-68873397	None:intergenic
GGCTCAGATTAAACTAGATG+AGG	0.594266	3.1:+68873306	MS.gene045214:CDS
TGAGGAGATTGAAGCAGCCA+TGG	0.603332	3.1:+68873324	MS.gene045214:CDS
AGGCCCAAGTCTGGAACAAG+AGG	0.603727	3.1:+68873734	MS.gene045214:CDS
GGAGGGGCGATAGCATCAAA+GGG	0.607813	3.1:-68873379	None:intergenic
CAAAGGGACTTAGGTTGCGA+CGG	0.608411	3.1:-68873363	None:intergenic
CTAAACCTGCTATTGCAGGA+GGG	0.624497	3.1:-68873396	None:intergenic
CGTCCAAAGCAAGTTTGGAG+AGG	0.664103	3.1:-68873471	None:intergenic
GCAATAGCAGGTTTAGCTGG+CGG	0.670820	3.1:+68873403	MS.gene045214:CDS
GGAGATTGAAGCAGCCATGG+CGG	0.715841	3.1:+68873327	MS.gene045214:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCTTATTTATTTATTTATCC+AGG	+	chr3.1:68873585-68873604	MS.gene045214:intron	15.0%
!	GAATTAATTAAGGAAGTAAC+TGG	-	chr3.1:68873533-68873552	None:intergenic	25.0%
!!!	ATCATTTGCTTTTGCTTTAA+AGG	-	chr3.1:68873683-68873702	None:intergenic	25.0%
!!!	CGATTTTTTGTTATGTCAAT+GGG	-	chr3.1:68873436-68873455	None:intergenic	25.0%
!	TCGCTTTGAGTATAAGAATA+AGG	+	chr3.1:68873285-68873304	MS.gene045214:CDS	30.0%
!!!	GCGATTTTTTGTTATGTCAA+TGG	-	chr3.1:68873437-68873456	None:intergenic	30.0%
	CAAACTCATAATTCGAATCC+TGG	-	chr3.1:68873606-68873625	None:intergenic	35.0%
	CGACTACAATCATAAGAATC+AGG	+	chr3.1:68873492-68873511	MS.gene045214:CDS	35.0%
	CGATCACACTTAATCACAAA+AGG	-	chr3.1:68873268-68873287	None:intergenic	35.0%
	CTGGAAAATTGTATCAGGAT+TGG	-	chr3.1:68873716-68873735	None:intergenic	35.0%
	TCAACTCGAAAATTGGTTAC+TGG	+	chr3.1:68873640-68873659	MS.gene045214:CDS	35.0%
	TGTCAAGTCAACTCGAAAAT+TGG	+	chr3.1:68873633-68873652	MS.gene045214:CDS	35.0%
	TTCAACTCAACATCTTCAAC+AGG	-	chr3.1:68873214-68873233	None:intergenic	35.0%
!	CAATCCTGATACAATTTTCC+AGG	+	chr3.1:68873714-68873733	MS.gene045214:CDS	35.0%
!!!	ATTTTCGAGTTGACTTGACA+AGG	-	chr3.1:68873633-68873652	None:intergenic	35.0%
	ACTCGAAAATTGGTTACTGG+TGG	+	chr3.1:68873643-68873662	MS.gene045214:CDS	40.0%
	CATTTCGGTTCCAATTTCAG+CGG	-	chr3.1:68873187-68873206	None:intergenic	40.0%
	GGCTCAGATTAAACTAGATG+AGG	+	chr3.1:68873306-68873325	MS.gene045214:CDS	40.0%
	TCTTCAACAGGTCTCCATTT+CGG	-	chr3.1:68873202-68873221	None:intergenic	40.0%
	AGACCTCTTGTTCCAGACTT+GGG	-	chr3.1:68873740-68873759	None:intergenic	45.0%
	CGATAGCATCAAAGGGACTT+AGG	-	chr3.1:68873375-68873394	None:intergenic	45.0%
	CGCTGAAATTGGAACCGAAA+TGG	+	chr3.1:68873185-68873204	MS.gene045214:CDS	45.0%
	CTAAACCTGCTATTGCAGGA+GGG	-	chr3.1:68873399-68873418	None:intergenic	45.0%
	GAATTGGATTCCCGCTTGTT+CGG	+	chr3.1:68873145-68873164	MS.gene045214:CDS	45.0%
	GTAGTCGTCCAAAGCAAGTT+TGG	-	chr3.1:68873479-68873498	None:intergenic	45.0%
	TAAACCTGCTATTGCAGGAG+GGG	-	chr3.1:68873398-68873417	None:intergenic	45.0%
	TGGGCCTGGAAAATTGTATC+AGG	-	chr3.1:68873721-68873740	None:intergenic	45.0%
!	TTGCCTCTCCAAACTTGCTT+TGG	+	chr3.1:68873468-68873487	MS.gene045214:CDS	45.0%
!!	TAATAATAATGAATTAATTA+AGG	-	chr3.1:68873543-68873562	None:intergenic	5.0%
	CAGACCTCTTGTTCCAGACT+TGG	-	chr3.1:68873741-68873760	None:intergenic	50.0%
	CCAGCTAAACCTGCTATTGC+AGG	-	chr3.1:68873403-68873422	None:intergenic	50.0%
	CCTGCAATAGCAGGTTTAGC+TGG	+	chr3.1:68873400-68873419	MS.gene045214:CDS	50.0%
	CGTCCAAAGCAAGTTTGGAG+AGG	-	chr3.1:68873474-68873493	None:intergenic	50.0%
	GCAATAGCAGGTTTAGCTGG+CGG	+	chr3.1:68873403-68873422	MS.gene045214:CDS	50.0%
	GCTAAACCTGCTATTGCAGG+AGG	-	chr3.1:68873400-68873419	None:intergenic	50.0%
	TGAGGAGATTGAAGCAGCCA+TGG	+	chr3.1:68873324-68873343	MS.gene045214:CDS	50.0%
	TTCGGTTCCAATTTCAGCGG+AGG	-	chr3.1:68873184-68873203	None:intergenic	50.0%
!	CAAAGGGACTTAGGTTGCGA+CGG	-	chr3.1:68873366-68873385	None:intergenic	50.0%
!	CAATTTTCCAGGCCCAAGTC+TGG	+	chr3.1:68873725-68873744	MS.gene045214:CDS	50.0%
	AGGAGGGGCGATAGCATCAA+AGG	-	chr3.1:68873383-68873402	None:intergenic	55.0%
	ATGGCACACGACTTGACCTC+AGG	-	chr3.1:68873766-68873785	None:intergenic	55.0%
	GAACAAGAGGTCTGCACCTG+AGG	+	chr3.1:68873747-68873766	MS.gene045214:CDS	55.0%
	GGAGATTGAAGCAGCCATGG+CGG	+	chr3.1:68873327-68873346	MS.gene045214:CDS	55.0%
	GGAGGGGCGATAGCATCAAA+GGG	-	chr3.1:68873382-68873401	None:intergenic	55.0%
	GGTTCCAATTTCAGCGGAGG+AGG	-	chr3.1:68873181-68873200	None:intergenic	55.0%
	GTCGAGTAACGCCGAACAAG+CGG	-	chr3.1:68873159-68873178	None:intergenic	55.0%
	TCGACCTCCTCCGCTGAAAT+TGG	+	chr3.1:68873174-68873193	MS.gene045214:CDS	55.0%
	TCGAGTAACGCCGAACAAGC+GGG	-	chr3.1:68873158-68873177	None:intergenic	55.0%
	TCTTGTTCCAGACTTGGGCC+TGG	-	chr3.1:68873735-68873754	None:intergenic	55.0%
!	AGGCCCAAGTCTGGAACAAG+AGG	+	chr3.1:68873734-68873753	MS.gene045214:CDS	55.0%
	ATCGCCCCTCCTGCAATAGC+AGG	+	chr3.1:68873391-68873410	MS.gene045214:CDS	60.0%
	GCGCTTGTCGTACTCCGCCA+TGG	-	chr3.1:68873344-68873363	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	68873136	68873795	68873136	ID=MS.gene045214
chr3.1	mRNA	68873136	68873795	68873136	ID=MS.gene045214.t1;Parent=MS.gene045214
chr3.1	exon	68873136	68873513	68873136	ID=MS.gene045214.t1.exon1;Parent=MS.gene045214.t1
chr3.1	CDS	68873136	68873513	68873136	ID=cds.MS.gene045214.t1;Parent=MS.gene045214.t1
chr3.1	exon	68873607	68873795	68873607	ID=MS.gene045214.t1.exon2;Parent=MS.gene045214.t1
chr3.1	CDS	68873607	68873795	68873607	ID=cds.MS.gene045214.t1;Parent=MS.gene045214.t1

Gene Sequence

>MS.gene045214

ATGTCGATGGAATTGGATTCCCGCTTGTTCGGCGTTACTCGACCTCCTCCGCTGAAATTGGAACCGAAATGGAGACCTGTTGAAGATGTTGAGTTGAAAGACTTCTCTATATATGATGATGACGTTGAGCCTTTTGTGATTAAGTGTGATCGCTTTGAGTATAAGAATAAGGCTCAGATTAAACTAGATGAGGAGATTGAAGCAGCCATGGCGGAGTACGACAAGCGCCGTCGCAACCTAAGTCCCTTTGATGCTATCGCCCCTCCTGCAATAGCAGGTTTAGCTGGCGGTATCAGACCCATTGACATAACAAAAAATCGCACACTTCTCACTTGCCTCTCCAAACTTGCTTTGGACGACTACAATCATAAGAATCAGGTACTAAACTTAATCACCAGTTACTTCCTTAATTAATTCATTATTATTATTATTATTCTAGTAATTCTTTTTCTTATTTATTTATTTATCCAGGATTCGAATTATGAGTTTGTTGACCTTGTCAAGTCAACTCGAAAATTGGTTACTGGTGGAATGTATTTCATTACCTTTAAAGCAAAAGCAAATGATGATGCTACTACCAATCCTGATACAATTTTCCAGGCCCAAGTCTGGAACAAGAGGTCTGCACCTGAGGTCAAGTCGTGTGCCATCAAAACCTAA

Protein sequence

>MS.gene045214.t1

MSMELDSRLFGVTRPPPLKLEPKWRPVEDVELKDFSIYDDDVEPFVIKCDRFEYKNKAQIKLDEEIEAAMAEYDKRRRNLSPFDAIAPPAIAGLAGGIRPIDITKNRTLLTCLSKLALDDYNHKNQDSNYEFVDLVKSTRKLVTGGMYFITFKAKANDDATTNPDTIFQAQVWNKRSAPEVKSCAIKT