Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70430.t1 | XP_004502163.1 | 54.9 | 182 | 72 | 6 | 70 | 249 | 89 | 262 | 1.50E-38 | 169.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70430.t1 | A0A1S2YB06 | 54.9 | 182 | 72 | 6 | 70 | 249 | 89 | 262 | 1.1e-38 | 169.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70430.t1 | MTR_3g083150 | 45.817 | 251 | 93 | 9 | 1 | 249 | 3 | 212 | 2.75e-52 | 169 |
| MS.gene70430.t1 | MTR_3g083030 | 40.081 | 247 | 123 | 7 | 5 | 249 | 7 | 230 | 1.24e-48 | 160 |
| MS.gene70430.t1 | MTR_3g083050 | 36.800 | 250 | 110 | 7 | 2 | 249 | 4 | 207 | 1.53e-38 | 134 |
| MS.gene70430.t1 | MTR_3g083100 | 53.957 | 139 | 44 | 7 | 123 | 249 | 44 | 174 | 3.85e-33 | 119 |
| MS.gene70430.t1 | MTR_3g083080 | 43.333 | 180 | 87 | 7 | 70 | 248 | 40 | 205 | 9.88e-31 | 113 |
| MS.gene70430.t1 | MTR_3g082340 | 35.968 | 253 | 126 | 10 | 1 | 248 | 1 | 222 | 1.93e-27 | 105 |
| MS.gene70430.t1 | MTR_3g082420 | 37.662 | 231 | 114 | 10 | 25 | 248 | 14 | 221 | 1.21e-26 | 103 |
| MS.gene70430.t1 | MTR_3g080680 | 35.749 | 207 | 106 | 9 | 54 | 248 | 7 | 198 | 3.36e-15 | 72.4 |
| MS.gene70430.t1 | MTR_3g086030 | 43.299 | 97 | 49 | 3 | 58 | 154 | 4 | 94 | 3.84e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 61 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAAAATCAAAATTGAAAT+TGG | 0.218152 | 3.1:-66218207 | MS.gene70430:CDS |
| TCAGGTTCCGGTTCCTGTTC+TGG | 0.268602 | 3.1:+66218296 | None:intergenic |
| ACATTTGCTTCGCGTGGAAT+AGG | 0.285666 | 3.1:+66217898 | None:intergenic |
| TGAACTCTCAAAACTTGCTT+TGG | 0.329305 | 3.1:-66217818 | MS.gene70430:CDS |
| ATCATCAATGTTAATGGGTC+TGG | 0.331844 | 3.1:+66217858 | None:intergenic |
| GCGACGATCATCAATGTTAA+TGG | 0.336654 | 3.1:+66217852 | None:intergenic |
| CACAACTTGACCTCAGCTTT+AGG | 0.366584 | 3.1:+66217512 | None:intergenic |
| CATTTGCTTCGCGTGGAATA+GGG | 0.382126 | 3.1:+66217899 | None:intergenic |
| TCCGAATCTGAGTCAGGTTC+CGG | 0.387483 | 3.1:+66218284 | None:intergenic |
| TCGATGGAGACTTCCGTGTT+TGG | 0.409673 | 3.1:-66218377 | MS.gene70430:CDS |
| ACGTACGCTTATGTTCTCTT+TGG | 0.417221 | 3.1:+66218007 | None:intergenic |
| TGGCGCTATCCGACCTCCCT+CGG | 0.430048 | 3.1:-66218357 | MS.gene70430:CDS |
| TCTGCTTCCGAATCTGAGTC+AGG | 0.441479 | 3.1:+66218278 | None:intergenic |
| AGAGGAATCGGAAGCAGAAT+CGG | 0.441498 | 3.1:-66218255 | MS.gene70430:CDS |
| TTGAGAGTTCAATGAAAAGT+TGG | 0.442717 | 3.1:+66217830 | None:intergenic |
| TTCGGAAGCAGAAGAGGAAT+CGG | 0.445225 | 3.1:-66218267 | MS.gene70430:CDS |
| CGACGATCATCAATGTTAAT+GGG | 0.446899 | 3.1:+66217853 | None:intergenic |
| TAAGTACAATCGCGACAATC+AGG | 0.448286 | 3.1:-66217794 | MS.gene70430:intron |
| GCTGAAACGTGAACCAGAAC+AGG | 0.451816 | 3.1:-66218309 | MS.gene70430:CDS |
| TGCCGTAGCTGCGTATTTGA+AGG | 0.453844 | 3.1:-66218030 | MS.gene70430:CDS |
| CTCCTCTTCAGCTCTTTCAC+TGG | 0.460728 | 3.1:+66218161 | None:intergenic |
| AAGGAACTCATACTTGATAT+CGG | 0.464780 | 3.1:+66217688 | None:intergenic |
| TCATCAATGTTAATGGGTCT+GGG | 0.481203 | 3.1:+66217859 | None:intergenic |
| ACGTGAACCAGAACAGGAAC+CGG | 0.488454 | 3.1:-66218303 | MS.gene70430:CDS |
| GCCTGAAAAGTTGTTGGAGC+AGG | 0.490523 | 3.1:+66217554 | None:intergenic |
| GGCGCTATCCGACCTCCCTC+GGG | 0.503278 | 3.1:-66218356 | MS.gene70430:CDS |
| TCGGGAAGCTGCGACGATAG+AGG | 0.503732 | 3.1:-66218338 | MS.gene70430:CDS |
| ACCGGAACCTGACTCAGATT+CGG | 0.504213 | 3.1:-66218285 | MS.gene70430:CDS |
| GGCCTTCAAATACGCAGCTA+CGG | 0.508382 | 3.1:+66218028 | None:intergenic |
| CGGGAAGCTGCGACGATAGA+GGG | 0.509892 | 3.1:-66218337 | MS.gene70430:CDS |
| CTCAGATTCGGAAGCAGAAG+AGG | 0.513582 | 3.1:-66218273 | MS.gene70430:CDS |
| AATTGAAATTGGATCTGTCT+TGG | 0.516569 | 3.1:-66218196 | MS.gene70430:CDS |
| TACCAGTGAAAGAGCTGAAG+AGG | 0.516791 | 3.1:-66218163 | MS.gene70430:CDS |
| ACGTGTGCCTGAAAAGTTGT+TGG | 0.530725 | 3.1:+66217548 | None:intergenic |
| AGCTAAGATAAAACAAGAAG+AGG | 0.538294 | 3.1:-66218063 | MS.gene70430:CDS |
| TTATTCTTGTACACAAATTG+CGG | 0.539548 | 3.1:+66218086 | None:intergenic |
| CGCAGCTTCCCGAGGGAGGT+CGG | 0.540112 | 3.1:+66218348 | None:intergenic |
| CACGCAAAATGAAAAGTACA+AGG | 0.542670 | 3.1:+66218110 | None:intergenic |
| TGGAATAGGGATGGCATCGA+AGG | 0.543328 | 3.1:+66217912 | None:intergenic |
| TCCTTCCTGTCTAATGTCGA+TGG | 0.544147 | 3.1:-66218393 | None:intergenic |
| ATCGGAAGCAGAATCGGAAT+CGG | 0.547771 | 3.1:-66218249 | MS.gene70430:CDS |
| CCACATACATTTGCTTCGCG+TGG | 0.552295 | 3.1:+66217892 | None:intergenic |
| TCTATCGTCGCAGCTTCCCG+AGG | 0.562694 | 3.1:+66218340 | None:intergenic |
| ATCGGAATCGGAAGAAGAAG+AGG | 0.567237 | 3.1:-66218237 | MS.gene70430:CDS |
| TACAAGGGTGATCATCATAC+CGG | 0.577686 | 3.1:+66218126 | None:intergenic |
| GTTGGAGCAGGGCAGTCTGA+AGG | 0.582271 | 3.1:+66217566 | None:intergenic |
| TGCTTCGCGTGGAATAGGGA+TGG | 0.588632 | 3.1:+66217903 | None:intergenic |
| CCACGCGAAGCAAATGTATG+TGG | 0.589475 | 3.1:-66217892 | MS.gene70430:CDS |
| TGAAATTGGATCTGTCTTGG+TGG | 0.593345 | 3.1:-66218193 | MS.gene70430:CDS |
| TCGTCGCAGCTTCCCGAGGG+AGG | 0.595927 | 3.1:+66218344 | None:intergenic |
| GAAATTGGATCTGTCTTGGT+GGG | 0.596722 | 3.1:-66218192 | MS.gene70430:CDS |
| AGAGGAGTGATTTCGCAAGC+CGG | 0.602949 | 3.1:-66218145 | MS.gene70430:CDS |
| CTATCGTCGCAGCTTCCCGA+GGG | 0.612642 | 3.1:+66218341 | None:intergenic |
| TGAGTTCCTTGAGCTTGTCA+AGG | 0.613030 | 3.1:-66217675 | MS.gene70430:CDS |
| GCTGCGACGATAGAGGGAAG+TGG | 0.614516 | 3.1:-66218331 | MS.gene70430:CDS |
| GGAATAGGGATGGCATCGAA+GGG | 0.630858 | 3.1:+66217913 | None:intergenic |
| AGGTCGGATAGCGCCAAACA+CGG | 0.633277 | 3.1:+66218364 | None:intergenic |
| CCTGAAAAGTTGTTGGAGCA+GGG | 0.635748 | 3.1:+66217555 | None:intergenic |
| GGGTTGCCTTGACAAGCTCA+AGG | 0.655608 | 3.1:+66217669 | None:intergenic |
| GAACTCGAAGCCTAAAGCTG+AGG | 0.667865 | 3.1:-66217522 | MS.gene70430:CDS |
| ACGCAAAATGAAAAGTACAA+GGG | 0.752956 | 3.1:+66218111 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTTTATCTTATTAATTTA+GGG | - | chr3.1:66218163-66218182 | MS.gene70430:CDS | 10.0% |
| !! | TTTGTTTATCTTATTAATTT+AGG | - | chr3.1:66218162-66218181 | MS.gene70430:CDS | 10.0% |
| !! | ATCAAAATCAAAATTGAAAT+TGG | - | chr3.1:66217669-66217688 | MS.gene70430:CDS | 15.0% |
| !! | AGAATTAAAAGCAAGATTAA+AGG | + | chr3.1:66217901-66217920 | None:intergenic | 20.0% |
| !! | ATGTAGTAATTACCAAAAAT+AGG | + | chr3.1:66218244-66218263 | None:intergenic | 20.0% |
| !! | TAATTACCAAAAATAGGAAT+AGG | + | chr3.1:66218238-66218257 | None:intergenic | 20.0% |
| ! | TTATTCTTGTACACAAATTG+CGG | + | chr3.1:66217793-66217812 | None:intergenic | 25.0% |
| AAGGAACTCATACTTGATAT+CGG | + | chr3.1:66218191-66218210 | None:intergenic | 30.0% | |
| AATTGAAATTGGATCTGTCT+TGG | - | chr3.1:66217680-66217699 | MS.gene70430:CDS | 30.0% | |
| AGCTAAGATAAAACAAGAAG+AGG | - | chr3.1:66217813-66217832 | MS.gene70430:CDS | 30.0% | |
| TTGAGAGTTCAATGAAAAGT+TGG | + | chr3.1:66218049-66218068 | None:intergenic | 30.0% | |
| ! | ACGCAAAATGAAAAGTACAA+GGG | + | chr3.1:66217768-66217787 | None:intergenic | 30.0% |
| !!! | TGTTTTTGGTTTTGCTTGAA+AGG | + | chr3.1:66218269-66218288 | None:intergenic | 30.0% |
| AAAAATAGGAATAGGACGAC+GGG | + | chr3.1:66218230-66218249 | None:intergenic | 35.0% | |
| CAAAAATAGGAATAGGACGA+CGG | + | chr3.1:66218231-66218250 | None:intergenic | 35.0% | |
| CGACGATCATCAATGTTAAT+GGG | + | chr3.1:66218026-66218045 | None:intergenic | 35.0% | |
| ! | ATCATCAATGTTAATGGGTC+TGG | + | chr3.1:66218021-66218040 | None:intergenic | 35.0% |
| ! | CACGCAAAATGAAAAGTACA+AGG | + | chr3.1:66217769-66217788 | None:intergenic | 35.0% |
| ! | TCAGTAGCATCTTCTGTTTT+TGG | + | chr3.1:66218283-66218302 | None:intergenic | 35.0% |
| ! | TCATCAATGTTAATGGGTCT+GGG | + | chr3.1:66218020-66218039 | None:intergenic | 35.0% |
| ! | TGAACTCTCAAAACTTGCTT+TGG | - | chr3.1:66218058-66218077 | MS.gene70430:CDS | 35.0% |
| ACGTACGCTTATGTTCTCTT+TGG | + | chr3.1:66217872-66217891 | None:intergenic | 40.0% | |
| GAAATTGGATCTGTCTTGGT+GGG | - | chr3.1:66217684-66217703 | MS.gene70430:CDS | 40.0% | |
| GCGACGATCATCAATGTTAA+TGG | + | chr3.1:66218027-66218046 | None:intergenic | 40.0% | |
| TAAGTACAATCGCGACAATC+AGG | - | chr3.1:66218082-66218101 | MS.gene70430:CDS | 40.0% | |
| TACAAGGGTGATCATCATAC+CGG | + | chr3.1:66217753-66217772 | None:intergenic | 40.0% | |
| TGAAATTGGATCTGTCTTGG+TGG | - | chr3.1:66217683-66217702 | MS.gene70430:CDS | 40.0% | |
| ! | CGTCGTCCTATTCCTATTTT+TGG | - | chr3.1:66218229-66218248 | MS.gene70430:CDS | 40.0% |
| ACATTTGCTTCGCGTGGAAT+AGG | + | chr3.1:66217981-66218000 | None:intergenic | 45.0% | |
| ACGTGTGCCTGAAAAGTTGT+TGG | + | chr3.1:66218331-66218350 | None:intergenic | 45.0% | |
| AGAGGAATCGGAAGCAGAAT+CGG | - | chr3.1:66217621-66217640 | MS.gene70430:CDS | 45.0% | |
| ATCGGAAGCAGAATCGGAAT+CGG | - | chr3.1:66217627-66217646 | MS.gene70430:CDS | 45.0% | |
| ATCGGAATCGGAAGAAGAAG+AGG | - | chr3.1:66217639-66217658 | MS.gene70430:CDS | 45.0% | |
| CACAACTTGACCTCAGCTTT+AGG | + | chr3.1:66218367-66218386 | None:intergenic | 45.0% | |
| CATTTGCTTCGCGTGGAATA+GGG | + | chr3.1:66217980-66217999 | None:intergenic | 45.0% | |
| CCTGAAAAGTTGTTGGAGCA+GGG | + | chr3.1:66218324-66218343 | None:intergenic | 45.0% | |
| TACCAGTGAAAGAGCTGAAG+AGG | - | chr3.1:66217713-66217732 | MS.gene70430:intron | 45.0% | |
| TGAGTTCCTTGAGCTTGTCA+AGG | - | chr3.1:66218201-66218220 | MS.gene70430:CDS | 45.0% | |
| TTCGGAAGCAGAAGAGGAAT+CGG | - | chr3.1:66217609-66217628 | MS.gene70430:CDS | 45.0% | |
| ACCGGAACCTGACTCAGATT+CGG | - | chr3.1:66217591-66217610 | MS.gene70430:CDS | 50.0% | |
| ACGTGAACCAGAACAGGAAC+CGG | - | chr3.1:66217573-66217592 | MS.gene70430:CDS | 50.0% | |
| AGAGGAGTGATTTCGCAAGC+CGG | - | chr3.1:66217731-66217750 | MS.gene70430:intron | 50.0% | |
| CCACATACATTTGCTTCGCG+TGG | + | chr3.1:66217987-66218006 | None:intergenic | 50.0% | |
| CCACGCGAAGCAAATGTATG+TGG | - | chr3.1:66217984-66218003 | MS.gene70430:intron | 50.0% | |
| CTCAGATTCGGAAGCAGAAG+AGG | - | chr3.1:66217603-66217622 | MS.gene70430:CDS | 50.0% | |
| CTCCTCTTCAGCTCTTTCAC+TGG | + | chr3.1:66217718-66217737 | None:intergenic | 50.0% | |
| GAACTCGAAGCCTAAAGCTG+AGG | - | chr3.1:66218354-66218373 | MS.gene70430:CDS | 50.0% | |
| GCCTGAAAAGTTGTTGGAGC+AGG | + | chr3.1:66218325-66218344 | None:intergenic | 50.0% | |
| GCTGAAACGTGAACCAGAAC+AGG | - | chr3.1:66217567-66217586 | MS.gene70430:CDS | 50.0% | |
| GGCCTTCAAATACGCAGCTA+CGG | + | chr3.1:66217851-66217870 | None:intergenic | 50.0% | |
| TCCGAATCTGAGTCAGGTTC+CGG | + | chr3.1:66217595-66217614 | None:intergenic | 50.0% | |
| TCGATGGAGACTTCCGTGTT+TGG | - | chr3.1:66217499-66217518 | MS.gene70430:CDS | 50.0% | |
| TCTGCTTCCGAATCTGAGTC+AGG | + | chr3.1:66217601-66217620 | None:intergenic | 50.0% | |
| TGCCGTAGCTGCGTATTTGA+AGG | - | chr3.1:66217846-66217865 | MS.gene70430:CDS | 50.0% | |
| ! | CCCTGCTCCAACAACTTTTC+AGG | - | chr3.1:66218321-66218340 | MS.gene70430:CDS | 50.0% |
| ! | GGAATAGGGATGGCATCGAA+GGG | + | chr3.1:66217966-66217985 | None:intergenic | 50.0% |
| ! | TGGAATAGGGATGGCATCGA+AGG | + | chr3.1:66217967-66217986 | None:intergenic | 50.0% |
| !! | CAACTTTTCAGGCACACGTC+TGG | - | chr3.1:66218332-66218351 | MS.gene70430:CDS | 50.0% |
| AGGTCGGATAGCGCCAAACA+CGG | + | chr3.1:66217515-66217534 | None:intergenic | 55.0% | |
| GGGTTGCCTTGACAAGCTCA+AGG | + | chr3.1:66218210-66218229 | None:intergenic | 55.0% | |
| TCAGGTTCCGGTTCCTGTTC+TGG | + | chr3.1:66217583-66217602 | None:intergenic | 55.0% | |
| TGCTTCGCGTGGAATAGGGA+TGG | + | chr3.1:66217976-66217995 | None:intergenic | 55.0% | |
| CGGGAAGCTGCGACGATAGA+GGG | - | chr3.1:66217539-66217558 | MS.gene70430:CDS | 60.0% | |
| CTATCGTCGCAGCTTCCCGA+GGG | + | chr3.1:66217538-66217557 | None:intergenic | 60.0% | |
| GCTGCGACGATAGAGGGAAG+TGG | - | chr3.1:66217545-66217564 | MS.gene70430:CDS | 60.0% | |
| GTTGGAGCAGGGCAGTCTGA+AGG | + | chr3.1:66218313-66218332 | None:intergenic | 60.0% | |
| TCGGGAAGCTGCGACGATAG+AGG | - | chr3.1:66217538-66217557 | MS.gene70430:CDS | 60.0% | |
| TCTATCGTCGCAGCTTCCCG+AGG | + | chr3.1:66217539-66217558 | None:intergenic | 60.0% | |
| TGGCGCTATCCGACCTCCCT+CGG | - | chr3.1:66217519-66217538 | MS.gene70430:CDS | 65.0% | |
| CGCAGCTTCCCGAGGGAGGT+CGG | + | chr3.1:66217531-66217550 | None:intergenic | 70.0% | |
| GGCGCTATCCGACCTCCCTC+GGG | - | chr3.1:66217520-66217539 | MS.gene70430:CDS | 70.0% | |
| TCGTCGCAGCTTCCCGAGGG+AGG | + | chr3.1:66217535-66217554 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 66217496 | 66218402 | 66217496 | ID=MS.gene70430 |
| chr3.1 | mRNA | 66217496 | 66218402 | 66217496 | ID=MS.gene70430.t1;Parent=MS.gene70430 |
| chr3.1 | exon | 66218013 | 66218402 | 66218013 | ID=MS.gene70430.t1.exon1;Parent=MS.gene70430.t1 |
| chr3.1 | CDS | 66218013 | 66218402 | 66218013 | ID=cds.MS.gene70430.t1;Parent=MS.gene70430.t1 |
| chr3.1 | exon | 66217795 | 66217935 | 66217795 | ID=MS.gene70430.t1.exon2;Parent=MS.gene70430.t1 |
| chr3.1 | CDS | 66217795 | 66217935 | 66217795 | ID=cds.MS.gene70430.t1;Parent=MS.gene70430.t1 |
| chr3.1 | exon | 66217496 | 66217714 | 66217496 | ID=MS.gene70430.t1.exon3;Parent=MS.gene70430.t1 |
| chr3.1 | CDS | 66217496 | 66217714 | 66217496 | ID=cds.MS.gene70430.t1;Parent=MS.gene70430.t1 |
| Gene Sequence |
| Protein sequence |
