Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044215.t1 | GAU51471.1 | 44.5 | 128 | 66 | 2 | 22 | 149 | 90 | 212 | 1.10E-16 | 97.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044215.t1 | A0A2Z6P4W7 | 44.5 | 128 | 66 | 2 | 22 | 149 | 90 | 212 | 7.9e-17 | 97.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene71282 | MS.gene044215 | PPI |
| MS.gene70424 | MS.gene044215 | PPI |
| MS.gene70427 | MS.gene044215 | PPI |
| MS.gene71528 | MS.gene044215 | PPI |
| MS.gene044376 | MS.gene044215 | PPI |
| MS.gene044363 | MS.gene044215 | PPI |
| MS.gene044215 | MS.gene044326 | PPI |
| MS.gene77464 | MS.gene044215 | PPI |
| MS.gene71284 | MS.gene044215 | PPI |
| MS.gene77472 | MS.gene044215 | PPI |
| MS.gene70418 | MS.gene044215 | PPI |
| MS.gene044215 | MS.gene049263 | PPI |
| MS.gene77468 | MS.gene044215 | PPI |
| MS.gene045214 | MS.gene044215 | PPI |
| MS.gene044215 | MS.gene044323 | PPI |
| MS.gene71531 | MS.gene044215 | PPI |
| MS.gene044361 | MS.gene044215 | PPI |
| MS.gene77466 | MS.gene044215 | PPI |
| MS.gene044370 | MS.gene044215 | PPI |
| MS.gene70476 | MS.gene044215 | PPI |
| MS.gene25704 | MS.gene044215 | PPI |
| MS.gene70443 | MS.gene044215 | PPI |
| MS.gene79115 | MS.gene044215 | PPI |
| MS.gene70430 | MS.gene044215 | PPI |
| MS.gene044215 | MS.gene71524 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044215.t1 | MTR_3g083080 | 43.478 | 138 | 66 | 4 | 5 | 136 | 57 | 188 | 4.73e-24 | 95.1 |
| MS.gene044215.t1 | MTR_3g080680 | 44.079 | 152 | 62 | 6 | 5 | 136 | 33 | 181 | 2.06e-22 | 90.5 |
| MS.gene044215.t1 | MTR_3g083050 | 41.611 | 149 | 62 | 5 | 12 | 143 | 55 | 195 | 3.67e-20 | 84.7 |
| MS.gene044215.t1 | MTR_3g083030 | 41.611 | 149 | 62 | 5 | 12 | 143 | 78 | 218 | 6.84e-20 | 84.7 |
| MS.gene044215.t1 | MTR_3g082420 | 42.593 | 108 | 58 | 3 | 33 | 136 | 97 | 204 | 9.89e-19 | 81.3 |
| MS.gene044215.t1 | MTR_3g082340 | 42.593 | 108 | 58 | 3 | 33 | 136 | 98 | 205 | 1.59e-18 | 80.9 |
| MS.gene044215.t1 | MTR_8g461300 | 44.355 | 124 | 53 | 6 | 22 | 134 | 61 | 179 | 4.00e-15 | 71.2 |
| MS.gene044215.t1 | MTR_4g055800 | 40.367 | 109 | 54 | 3 | 28 | 134 | 171 | 270 | 3.09e-14 | 70.5 |
| MS.gene044215.t1 | MTR_3g083150 | 38.028 | 142 | 74 | 6 | 4 | 135 | 57 | 194 | 2.78e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTCTGAGCTTGCTTTGA+AGG | 0.280067 | 3.3:+67910623 | MS.gene044215:CDS |
| GTCAGCAATTAGTTCTTCTA+TGG | 0.315331 | 3.3:-67910376 | None:intergenic |
| GATTTAAAATTTAAGCAAAT+CGG | 0.374445 | 3.3:+67911120 | MS.gene044215:CDS |
| GAGAGTAGGGCGAAGATCTT+CGG | 0.406860 | 3.3:-67910595 | None:intergenic |
| CCATGCTATAACAACTTTCC+AGG | 0.412488 | 3.3:+67910876 | MS.gene044215:CDS |
| TTTGTTTGTTGGAAATACTT+TGG | 0.419780 | 3.3:+67911312 | MS.gene044215:CDS |
| ATTTAAAATTTAAGCAAATC+GGG | 0.423979 | 3.3:+67911121 | MS.gene044215:CDS |
| GTTCGACGATGACGACTTTG+CGG | 0.439069 | 3.3:+67910341 | MS.gene044215:CDS |
| GTCAAGTGTACCACTGCTAT+TGG | 0.447420 | 3.3:+67910787 | MS.gene044215:CDS |
| GGATTACAATCAAAAGAATC+AGG | 0.463360 | 3.3:+67910644 | MS.gene044215:CDS |
| GTCGTCATCGTCATCGTCCA+TGG | 0.476185 | 3.3:-67910298 | None:intergenic |
| ACTGCTATTGGTTGTGGTGG+TGG | 0.480634 | 3.3:+67910799 | MS.gene044215:CDS |
| CAGAGAGAGTAACGAGAGTA+GGG | 0.497560 | 3.3:-67910608 | None:intergenic |
| TCAGAGAGAGTAACGAGAGT+AGG | 0.497887 | 3.3:-67910609 | None:intergenic |
| AGTTGTTATAGCATGGGTGT+TGG | 0.503404 | 3.3:-67910869 | None:intergenic |
| CCACATAACTCTAGAACGGC+TGG | 0.510258 | 3.3:-67910549 | None:intergenic |
| TGTACCACTGCTATTGGTTG+TGG | 0.522647 | 3.3:+67910793 | MS.gene044215:CDS |
| TTTGTTGGAAATACTTTGGA+TGG | 0.526260 | 3.3:+67911316 | MS.gene044215:CDS |
| AAGATCTTCGGTTATGTCAA+AGG | 0.527327 | 3.3:-67910583 | None:intergenic |
| CCTGGAAAGTTGTTATAGCA+TGG | 0.530741 | 3.3:-67910876 | None:intergenic |
| GCAGCGTAAGGTGGAAAGCA+AGG | 0.537631 | 3.3:-67911150 | None:intergenic |
| CGACTTTGCGGAAGATAAAG+AGG | 0.546436 | 3.3:+67910353 | MS.gene044215:CDS |
| AGATCTTCGGTTATGTCAAA+GGG | 0.549668 | 3.3:-67910582 | None:intergenic |
| GTTATAGCATGGGTGTTGGA+AGG | 0.561860 | 3.3:-67910865 | None:intergenic |
| CAGCGTAAGGTGGAAAGCAA+GGG | 0.568224 | 3.3:-67911149 | None:intergenic |
| CATCATCCCATACTTCAGCC+TGG | 0.568718 | 3.3:-67910894 | None:intergenic |
| GCAGTGGTTAACATTTGCTG+TGG | 0.571386 | 3.3:+67911098 | MS.gene044215:intron |
| CTGGAAAGTTGTTATAGCAT+GGG | 0.572374 | 3.3:-67910875 | None:intergenic |
| ACCACTGCTATTGGTTGTGG+TGG | 0.575309 | 3.3:+67910796 | MS.gene044215:CDS |
| CCAGCCGTTCTAGAGTTATG+TGG | 0.626689 | 3.3:+67910549 | MS.gene044215:CDS |
| ACACACAAGAGATTCAGCCT+TGG | 0.631252 | 3.3:-67911217 | None:intergenic |
| ACAGCCACATAACTCTAGAA+CGG | 0.642024 | 3.3:-67910553 | None:intergenic |
| TAGCAGTGGTACACTTGACA+AGG | 0.661404 | 3.3:-67910783 | None:intergenic |
| GGATGATAGATAGCATCGAA+CGG | 0.667704 | 3.3:-67910528 | None:intergenic |
| ACCACCACAACCAATAGCAG+TGG | 0.671651 | 3.3:-67910797 | None:intergenic |
| AAAGTCGTCATCGTCGAACG+TGG | 0.686780 | 3.3:-67910337 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATCTCAAAGAAAATATTA+AGG | - | chr3.3:67910996-67911015 | None:intergenic | 15.0% |
| !!! | ATTTAAAATTTAAGCAAATC+GGG | + | chr3.3:67911121-67911140 | MS.gene044215:CDS | 15.0% |
| !!! | GATTTAAAATTTAAGCAAAT+CGG | + | chr3.3:67911120-67911139 | MS.gene044215:CDS | 15.0% |
| !!! | TTTTAAATGGTTATAGTTTA+TGG | + | chr3.3:67911014-67911033 | MS.gene044215:intron | 15.0% |
| !!! | TTTTCTTTGAGATTTTTAAA+TGG | + | chr3.3:67911001-67911020 | MS.gene044215:intron | 15.0% |
| !! | AATATATGTAGCAAAATCAA+AGG | - | chr3.3:67911042-67911061 | None:intergenic | 20.0% |
| !! | GGAATTAATTATTATTACCA+AGG | - | chr3.3:67910971-67910990 | None:intergenic | 20.0% |
| !! | GTTCTTCTTCTTAATTATTA+GGG | + | chr3.3:67910739-67910758 | MS.gene044215:intron | 20.0% |
| !! | TGAAACATGATAAAATTGAA+TGG | - | chr3.3:67910475-67910494 | None:intergenic | 20.0% |
| !! | TGTTCTTCTTCTTAATTATT+AGG | + | chr3.3:67910738-67910757 | MS.gene044215:intron | 20.0% |
| !!! | TCAAATTAGATTTTGTTTGT+TGG | + | chr3.3:67911301-67911320 | MS.gene044215:CDS | 20.0% |
| ! | AATTATTATTACCAAGGTAG+TGG | - | chr3.3:67910965-67910984 | None:intergenic | 25.0% |
| ! | ATAGAAAAAAGATAGAACAG+TGG | - | chr3.3:67911191-67911210 | None:intergenic | 25.0% |
| ! | CAATTATTCATCAACTAATC+TGG | + | chr3.3:67910705-67910724 | MS.gene044215:intron | 25.0% |
| ! | CTCAAAGAAAATATTAAGGA+TGG | - | chr3.3:67910992-67911011 | None:intergenic | 25.0% |
| !! | TTTGTTTGTTGGAAATACTT+TGG | + | chr3.3:67911312-67911331 | MS.gene044215:CDS | 25.0% |
| AAAGATAGATTCGTACACTT+AGG | - | chr3.3:67910422-67910441 | None:intergenic | 30.0% | |
| GGATTACAATCAAAAGAATC+AGG | + | chr3.3:67910644-67910663 | MS.gene044215:CDS | 30.0% | |
| TAAAATCTAATCCACTACCT+TGG | + | chr3.3:67910951-67910970 | MS.gene044215:intron | 30.0% | |
| TTTGTTGGAAATACTTTGGA+TGG | + | chr3.3:67911316-67911335 | MS.gene044215:CDS | 30.0% | |
| !!! | GGTAGTGGATTAGATTTTAA+TGG | - | chr3.3:67910950-67910969 | None:intergenic | 30.0% |
| AAGATCTTCGGTTATGTCAA+AGG | - | chr3.3:67910586-67910605 | None:intergenic | 35.0% | |
| AGATCTTCGGTTATGTCAAA+GGG | - | chr3.3:67910585-67910604 | None:intergenic | 35.0% | |
| CTGGAAAGTTGTTATAGCAT+GGG | - | chr3.3:67910878-67910897 | None:intergenic | 35.0% | |
| ! | ATATTTGTTTGTTGCTGCAG+TGG | + | chr3.3:67911082-67911101 | MS.gene044215:intron | 35.0% |
| ! | GTCAGCAATTAGTTCTTCTA+TGG | - | chr3.3:67910379-67910398 | None:intergenic | 35.0% |
| !! | TTCTTCTGCTTTTGCTTGAA+AGG | - | chr3.3:67910842-67910861 | None:intergenic | 35.0% |
| !!! | AACATTGTTTTTGCAGCGTA+AGG | - | chr3.3:67911165-67911184 | None:intergenic | 35.0% |
| !!! | CTTTTTTCTATTTGACGCCA+AGG | + | chr3.3:67911200-67911219 | MS.gene044215:CDS | 35.0% |
| AACTTTCCAGGCTGAAGTAT+GGG | + | chr3.3:67910888-67910907 | MS.gene044215:intron | 40.0% | |
| AGTTGTTATAGCATGGGTGT+TGG | - | chr3.3:67910872-67910891 | None:intergenic | 40.0% | |
| CCATGCTATAACAACTTTCC+AGG | + | chr3.3:67910876-67910895 | MS.gene044215:CDS | 40.0% | |
| CCTGGAAAGTTGTTATAGCA+TGG | - | chr3.3:67910879-67910898 | None:intergenic | 40.0% | |
| ! | ACAGCCACATAACTCTAGAA+CGG | - | chr3.3:67910556-67910575 | None:intergenic | 40.0% |
| ! | GGATGATAGATAGCATCGAA+CGG | - | chr3.3:67910531-67910550 | None:intergenic | 40.0% |
| !!! | ATTGTTTTTGCAGCGTAAGG+TGG | - | chr3.3:67911162-67911181 | None:intergenic | 40.0% |
| ACACACAAGAGATTCAGCCT+TGG | - | chr3.3:67911220-67911239 | None:intergenic | 45.0% | |
| CAACTTTCCAGGCTGAAGTA+TGG | + | chr3.3:67910887-67910906 | MS.gene044215:intron | 45.0% | |
| CAGAGAGAGTAACGAGAGTA+GGG | - | chr3.3:67910611-67910630 | None:intergenic | 45.0% | |
| CGACTTTGCGGAAGATAAAG+AGG | + | chr3.3:67910353-67910372 | MS.gene044215:CDS | 45.0% | |
| GTCAAGTGTACCACTGCTAT+TGG | + | chr3.3:67910787-67910806 | MS.gene044215:CDS | 45.0% | |
| TAGCAGTGGTACACTTGACA+AGG | - | chr3.3:67910786-67910805 | None:intergenic | 45.0% | |
| TCAGAGAGAGTAACGAGAGT+AGG | - | chr3.3:67910612-67910631 | None:intergenic | 45.0% | |
| ! | GCAGTGGTTAACATTTGCTG+TGG | + | chr3.3:67911098-67911117 | MS.gene044215:intron | 45.0% |
| ! | TCTCTCTGAGCTTGCTTTGA+AGG | + | chr3.3:67910623-67910642 | MS.gene044215:CDS | 45.0% |
| ! | TGTACCACTGCTATTGGTTG+TGG | + | chr3.3:67910793-67910812 | MS.gene044215:CDS | 45.0% |
| !! | GTTATAGCATGGGTGTTGGA+AGG | - | chr3.3:67910868-67910887 | None:intergenic | 45.0% |
| AAAGTCGTCATCGTCGAACG+TGG | - | chr3.3:67910340-67910359 | None:intergenic | 50.0% | |
| ACCACCACAACCAATAGCAG+TGG | - | chr3.3:67910800-67910819 | None:intergenic | 50.0% | |
| CAGCGTAAGGTGGAAAGCAA+GGG | - | chr3.3:67911152-67911171 | None:intergenic | 50.0% | |
| CATCATCCCATACTTCAGCC+TGG | - | chr3.3:67910897-67910916 | None:intergenic | 50.0% | |
| GAGAGTAGGGCGAAGATCTT+CGG | - | chr3.3:67910598-67910617 | None:intergenic | 50.0% | |
| GTTCGACGATGACGACTTTG+CGG | + | chr3.3:67910341-67910360 | MS.gene044215:CDS | 50.0% | |
| ! | ACCACTGCTATTGGTTGTGG+TGG | + | chr3.3:67910796-67910815 | MS.gene044215:CDS | 50.0% |
| ! | ACTGCTATTGGTTGTGGTGG+TGG | + | chr3.3:67910799-67910818 | MS.gene044215:CDS | 50.0% |
| !! | CCACATAACTCTAGAACGGC+TGG | - | chr3.3:67910552-67910571 | None:intergenic | 50.0% |
| !! | CCAGCCGTTCTAGAGTTATG+TGG | + | chr3.3:67910549-67910568 | MS.gene044215:CDS | 50.0% |
| GCAGCGTAAGGTGGAAAGCA+AGG | - | chr3.3:67911153-67911172 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 67910300 | 67911341 | 67910300 | ID=MS.gene044215 |
| chr3.3 | mRNA | 67910300 | 67911341 | 67910300 | ID=MS.gene044215.t1;Parent=MS.gene044215 |
| chr3.3 | exon | 67910300 | 67910425 | 67910300 | ID=MS.gene044215.t1.exon1;Parent=MS.gene044215.t1 |
| chr3.3 | CDS | 67910300 | 67910425 | 67910300 | ID=cds.MS.gene044215.t1;Parent=MS.gene044215.t1 |
| chr3.3 | exon | 67910528 | 67910665 | 67910528 | ID=MS.gene044215.t1.exon2;Parent=MS.gene044215.t1 |
| chr3.3 | CDS | 67910528 | 67910665 | 67910528 | ID=cds.MS.gene044215.t1;Parent=MS.gene044215.t1 |
| chr3.3 | exon | 67910760 | 67910903 | 67910760 | ID=MS.gene044215.t1.exon3;Parent=MS.gene044215.t1 |
| chr3.3 | CDS | 67910760 | 67910903 | 67910760 | ID=cds.MS.gene044215.t1;Parent=MS.gene044215.t1 |
| chr3.3 | exon | 67911102 | 67911341 | 67911102 | ID=MS.gene044215.t1.exon4;Parent=MS.gene044215.t1 |
| chr3.3 | CDS | 67911102 | 67911341 | 67911102 | ID=cds.MS.gene044215.t1;Parent=MS.gene044215.t1 |
| Gene Sequence |
| Protein sequence |
